Shrm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shrm
Entrez Gene ID	36592
Full Name	CG34379 gene product from transcript CG34379-RH
Synonyms	CG13942,CG13944,CG34379,CG8603,Dmel\CG34379,Dmel_CG13942,Dmel_CG8603,Shrm,Shrooom,dShrm,dmShroom
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50F1-50F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103837.2	NP_001097307.1	shroom, isoform D
NM_001299488.1	NP_001286417.1	shroom, isoform H
NM_137108.3	NP_610952.1	shroom, isoform F
NM_166047.2	NP_725378.2	shroom, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The structure of Shrm domain 2 (SD2), which mediates the interaction with Rock and is required for Shrm function, is reported.
Title: Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction.

The data show that the differential subcellular distribution of naturally occurring isoforms of Shroom proteins can define both the position and organization of actomyosin networks in vivo.
Title: Specific isoforms of drosophila shroom define spatial requirements for the induction of apical constriction.

The following Shrm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shrm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09968	NM_166047.2 Latest version!	Drosophila melanogaster shroom, transcript variant G (Shrm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09969	NM_137108.3 Latest version!	Drosophila melanogaster shroom, transcript variant F (Shrm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09967	NM_001299488.1 Latest version!	Drosophila melanogaster shroom, transcript variant H (Shrm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa09967	NM_001103837.2 Latest version!	Drosophila melanogaster shroom, transcript variant D (Shrm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shrm ( NM_166047.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725378.2 Drosophila melanogaster shroom, transcript variant G (Shrm), mRNA.

Price & Availability↑

CloneID	OFa09968
Clone ID Related Accession (Same CDS sequence)	NM_166047.2
Accession Version	NM_166047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shroom, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166047.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTTCG GCGGCAAGTT TCGAATCAGA AGATCGAATT CCAAGGCCTC ATACTTGCCG 
CGTCAGAGTC TGGAGAAGTT GAACAACACT GATCCCGACC ATGGCATATA CAAGCTCACC 
CTGACCTCCA ACGAGGACTT GGTGGCCCAC ACGAAGCCCA GCTATGGGGT CACAGGAAAG 
CTGCCCAACA ATCTGCCGGA TGTCCTGCCG CTGGGCGTTA AGCTCCACCA GCAGCCAAAG 
TTGCAGCCAG GATCGCCGAA CGGCGATGCG AATGTGACCC TGCGCTATGG CTCCAACAAC 
AATCTGACTG GGAATTCCCC GACGGTTGCC CCGCCCCCCT ACTATGGGGG CGGCCAGCGG 
TATTCAACTC CTGTGCTGGG TCAAGGTTAC GGCAAAAGTT CGAAGCCCGT GACCCCGCAA 
CAATATACGA GATCTCAGTC GTACGATGTG AAGCACACTA GTGCGGTGAC TATGCCGACA 
ATGTCCCAGT CCCACGTGGA TCTCAAGCAG GCCGCCCATG ACCTAGAGAC GACGCTGGAG 
GAGGTGCTGC CCACTGCCAC GCCCACGCCG ACGCCAACAC CGACGCCCAC ACCGCCACGC 
CTCTCGCCGG CTTCCTCGCA CTCGGACTGC AGTCTGAGCA CCAGTTCCTT GGAGTGCACA 
ATCAATCCTA TAGCGACACC GATTCCTAAG CCTGAGGCGC ACATCTTTCG CGCCGAGGTG 
ATTAGCACCA CCCTGAACAC AAATCCGTTG ACAACACCGC CCAAGCCCGC GATGAACCGC 
CAGGAATCCC TGAGGGAGAA CATCGAAAAG ATCACCCAAC TACAGTCGGT GCTGATGTCG 
GCGCACCTGT GTGATGCGAG TCTACTAGGT GGTTACACCA CTCCACTGAT AACCAGTCCC 
ACTGCCAGTT TCGCTAACGA ACCACTAATG ACACCACCAC TGCCGCCCAG TCCGCCACCG 
CCACTAGAAC CGGAGGAGGA GGAGGAGCAG GAGGAGAACG ATGTGCACGA CAAGCAGCCA 
GAGATCGAGG AACTGCAGCT GATGCAGCGC AGCGAATTGG TCCTAATGGT GAATCCCAAG 
CCGAGCACAA CGGATATGGC CTGCCAAACG GACGAGCTGG AGGACAGGGA CACGGACCTC 
GAAGCGGCAC GCGAGGAGCA CCAGACTAGA ACGACTCTGC AGCCGCGACA GCGCCAGCCC 
ATCGAGCTGG ACTACGAGCA GATGAGCCGG GAGCTGGTTA AGCTCCTACC GCCTGGTGAC 
AAGATCGCCG ACATCCTCAC ACCAAAGATC TGCAAGCCCA CCTCGCAATA CGTTAGCAAT 
CTGTACAATC CGGATGTGCC ACTGCGCTTG GCCAAGCGCG ATGTTGGCAC CTCTACGTTG 
ATGCGAATGA AGTCCATCAC GTCGTCTGCC GAGATCCGAG TGGTCAGTGT GGAGCTGCAG 
CTGGCAGAGC CGAGCGAGGA GCCGACGAAT TTAATCAAGC AAAAGATGGA TGAGCTCATC 
AAGCATTTGA ACCAAAAAAT TGTCTCCCTG AAACGCGAGC AGCAGACGAT CAGCGAGGAG 
TGCTCGGCCA ATGACAGACT GGGCCAGGAT CTATTCGCCA AGCTAGCGGA GAAGGTTCGA 
CCCAGCGAAG CCTCCAAGTT CCGTACCCAT GTCGACGCCG TGGGCAACAT AACCAGTTTA 
CTTCTGTCGC TTTCCGAGCG TTTGGCCCAA ACCGAAAGCA GCCTGGAAAC GCGCCAGCAG 
GAAAGGGGCG CGCTGGAATC AAAGCGGGAT CTGCTGTACG AGCAGATGGA GGAGGCGCAG 
CGTCTCAAAT CGGACATAGA ACGACGTGGA GTCAGCATCG CCGGATTACT GGCCAAGAAC 
CTCAGCGCGG ACATGTGCGC CGACTACGAC TACTTCATCA ACATGAAGGC CAAGCTGATC 
GCCGATGCAC GCGACCTGGC CGTAAGGATC AAGGGCAGCG AGGAGCAGCT TAGCTCCCTC 
AGCGATGCGC TAGTCCAAAG CGATTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725378.2
CDS	506..2515
Translation	MVFGGKFRIRRSNSKASYLPRQSLEKLNNTDPDHGIYKLTLTSNEDLVAHTKPSYGVTGKLPNNLPDVLPLGVKLHQQPKLQPGSPNGDANVTLRYGSNNNLTGNSPTVAPPPYYGGGQRYSTPVLGQGYGKSSKPVTPQQYTRSQSYDVKHTSAVTMPTMSQSHVDLKQAAHDLETTLEEVLPTATPTPTPTPTPTPPRLSPASSHSDCSLSTSSLECTINPIATPIPKPEAHIFRAEVISTTLNTNPLTTPPKPAMNRQESLRENIEKITQLQSVLMSAHLCDASLLGGYTTPLITSPTASFANEPLMTPPLPPSPPPPLEPEEEEEQEENDVHDKQPEIEELQLMQRSELVLMVNPKPSTTDMACQTDELEDRDTDLEAAREEHQTRTTLQPRQRQPIELDYEQMSRELVKLLPPGDKIADILTPKICKPTSQYVSNLYNPDVPLRLAKRDVGTSTLMRMKSITSSAEIRVVSVELQLAEPSEEPTNLIKQKMDELIKHLNQKIVSLKREQQTISEECSANDRLGQDLFAKLAEKVRPSEASKFRTHVDAVGNITSLLLSLSERLAQTESSLETRQQERGALESKRDLLYEQMEEAQRLKSDIERRGVSIAGLLAKNLSADMCADYDYFINMKAKLIADARDLAVRIKGSEEQLSSLSDALVQSDC

Target ORF information:

RefSeq Version	NM_166047.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shroom, transcript variant G (Shrm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166047.2

ORF Insert Sequence:

ATGGTGTTCG GCGGCAAGTT TCGAATCAGA AGATCGAATT CCAAGGCCTC ATACTTGCCG 
CGTCAGAGTC TGGAGAAGTT GAACAACACT GATCCCGACC ATGGCATATA CAAGCTCACC 
CTGACCTCCA ACGAGGACTT GGTGGCCCAC ACGAAGCCCA GCTATGGGGT CACAGGAAAG 
CTGCCCAACA ATCTGCCGGA TGTCCTGCCG CTGGGCGTTA AGCTCCACCA GCAGCCAAAG 
TTGCAGCCAG GATCGCCGAA CGGCGATGCG AATGTGACCC TGCGCTATGG CTCCAACAAC 
AATCTGACTG GGAATTCCCC GACGGTTGCC CCGCCCCCCT ACTATGGGGG CGGCCAGCGG 
TATTCAACTC CTGTGCTGGG TCAAGGTTAC GGCAAAAGTT CGAAGCCCGT GACCCCGCAA 
CAATATACGA GATCTCAGTC GTACGATGTG AAGCACACTA GTGCGGTGAC TATGCCGACA 
ATGTCCCAGT CCCACGTGGA TCTCAAGCAG GCCGCCCATG ACCTAGAGAC GACGCTGGAG 
GAGGTGCTGC CCACTGCCAC GCCCACGCCG ACGCCAACAC CGACGCCCAC ACCGCCACGC 
CTCTCGCCGG CTTCCTCGCA CTCGGACTGC AGTCTGAGCA CCAGTTCCTT GGAGTGCACA 
ATCAATCCTA TAGCGACACC GATTCCTAAG CCTGAGGCGC ACATCTTTCG CGCCGAGGTG 
ATTAGCACCA CCCTGAACAC AAATCCGTTG ACAACACCGC CCAAGCCCGC GATGAACCGC 
CAGGAATCCC TGAGGGAGAA CATCGAAAAG ATCACCCAAC TACAGTCGGT GCTGATGTCG 
GCGCACCTGT GTGATGCGAG TCTACTAGGT GGTTACACCA CTCCACTGAT AACCAGTCCC 
ACTGCCAGTT TCGCTAACGA ACCACTAATG ACACCACCAC TGCCGCCCAG TCCGCCACCG 
CCACTAGAAC CGGAGGAGGA GGAGGAGCAG GAGGAGAACG ATGTGCACGA CAAGCAGCCA 
GAGATCGAGG AACTGCAGCT GATGCAGCGC AGCGAATTGG TCCTAATGGT GAATCCCAAG 
CCGAGCACAA CGGATATGGC CTGCCAAACG GACGAGCTGG AGGACAGGGA CACGGACCTC 
GAAGCGGCAC GCGAGGAGCA CCAGACTAGA ACGACTCTGC AGCCGCGACA GCGCCAGCCC 
ATCGAGCTGG ACTACGAGCA GATGAGCCGG GAGCTGGTTA AGCTCCTACC GCCTGGTGAC 
AAGATCGCCG ACATCCTCAC ACCAAAGATC TGCAAGCCCA CCTCGCAATA CGTTAGCAAT 
CTGTACAATC CGGATGTGCC ACTGCGCTTG GCCAAGCGCG ATGTTGGCAC CTCTACGTTG 
ATGCGAATGA AGTCCATCAC GTCGTCTGCC GAGATCCGAG TGGTCAGTGT GGAGCTGCAG 
CTGGCAGAGC CGAGCGAGGA GCCGACGAAT TTAATCAAGC AAAAGATGGA TGAGCTCATC 
AAGCATTTGA ACCAAAAAAT TGTCTCCCTG AAACGCGAGC AGCAGACGAT CAGCGAGGAG 
TGCTCGGCCA ATGACAGACT GGGCCAGGAT CTATTCGCCA AGCTAGCGGA GAAGGTTCGA 
CCCAGCGAAG CCTCCAAGTT CCGTACCCAT GTCGACGCCG TGGGCAACAT AACCAGTTTA 
CTTCTGTCGC TTTCCGAGCG TTTGGCCCAA ACCGAAAGCA GCCTGGAAAC GCGCCAGCAG 
GAAAGGGGCG CGCTGGAATC AAAGCGGGAT CTGCTGTACG AGCAGATGGA GGAGGCGCAG 
CGTCTCAAAT CGGACATAGA ACGACGTGGA GTCAGCATCG CCGGATTACT GGCCAAGAAC 
CTCAGCGCGG ACATGTGCGC CGACTACGAC TACTTCATCA ACATGAAGGC CAAGCTGATC 
GCCGATGCAC GCGACCTGGC CGTAAGGATC AAGGGCAGCG AGGAGCAGCT TAGCTCCCTC 
AGCGATGCGC TAGTCCAAAG CGATTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shrm ( NM_137108.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610952.1 Drosophila melanogaster shroom, transcript variant F (Shrm), mRNA.

Price & Availability↑

CloneID	OFa09969
Clone ID Related Accession (Same CDS sequence)	NM_137108.3
Accession Version	NM_137108.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shroom, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137108.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGT GGTTTCAATG GCTCAACTTT CGATCGAATT CCAAGGCCTC ATACTTGCCG 
CGTCAGAGTC TGGAGAAGTT GAACAACACT GATCCCGACC ATGGCATATA CAAGCTCACC 
CTGACCTCCA ACGAGGACTT GGTGGCCCAC ACGAAGCCCA GCTATGGGGT CACAGGAAAG 
CTGCCCAACA ATCTGCCGGA TGTCCTGCCG CTGGGCGTTA AGCTCCACCA GCAGCCAAAG 
TTGCAGCCAG GATCGCCGAA CGGCGATGCG AATGTGACCC TGCGCTATGG CTCCAACAAC 
AATCTGACTG GGAATTCCCC GACGGTTGCC CCGCCCCCCT ACTATGGGGG CGGCCAGCGG 
TATTCAACTC CTGTGCTGGG TCAAGGTTAC GGCAAAAGTT CGAAGCCCGT GACCCCGCAA 
CAATATACGA GATCTCAGTC GTACGATGTG AAGCACACTA GTGCGGTGAC TATGCCGACA 
ATGTCCCAGT CCCACGTGGA TCTCAAGCAG GCCGCCCATG ACCTAGAGAC GACGCTGGAG 
GAGGTGCTGC CCACTGCCAC GCCCACGCCG ACGCCAACAC CGACGCCCAC ACCGCCACGC 
CTCTCGCCGG CTTCCTCGCA CTCGGACTGC AGTCTGAGCA CCAGTTCCTT GGAGTGCACA 
ATCAATCCTA TAGCGACACC GATTCCTAAG CCTGAGGCGC ACATCTTTCG CGCCGAGGTG 
ATTAGCACCA CCCTGAACAC AAATCCGTTG ACAACACCGC CCAAGCCCGC GATGAACCGC 
CAGGAATCCC TGAGGGAGAA CATCGAAAAG ATCACCCAAC TACAGTCGGT GCTGATGTCG 
GCGCACCTGT GTGATGCGAG TCTACTAGGT GGTTACACCA CTCCACTGAT AACCAGTCCC 
ACTGCCAGTT TCGCTAACGA ACCACTAATG ACACCACCAC TGCCGCCCAG TCCGCCACCG 
CCACTAGAAC CGGAGGAGGA GGAGGAGCAG GAGGAGAACG ATGTGCACGA CAAGCAGCCA 
GAGATCGAGG AACTGCAGCT GATGCAGCGC AGCGAATTGG TCCTAATGGT GAATCCCAAG 
CCGAGCACAA CGGATATGGC CTGCCAAACG GACGAGCTGG AGGACAGGGA CACGGACCTC 
GAAGCGGCAC GCGAGGAGCA CCAGACTAGA ACGACTCTGC AGCCGCGACA GCGCCAGCCC 
ATCGAGCTGG ACTACGAGCA GATGAGCCGG GAGCTGGTTA AGCTCCTACC GCCTGGTGAC 
AAGATCGCCG ACATCCTCAC ACCAAAGATC TGCAAGCCCA CCTCGCAATA CGTTAGCAAT 
CTGTACAATC CGGATGTGCC ACTGCGCTTG GCCAAGCGCG ATGTTGGCAC CTCTACGTTG 
ATGCGAATGA AGTCCATCAC GTCGTCTGCC GAGATCCGAG TGGTCAGTGT GGAGCTGCAG 
CTGGCAGAGC CGAGCGAGGA GCCGACGAAT TTAATCAAGC AAAAGATGGA TGAGCTCATC 
AAGCATTTGA ACCAAAAAAT TGTCTCCCTG AAACGCGAGC AGCAGACGAT CAGCGAGGAG 
TGCTCGGCCA ATGACAGACT GGGCCAGGAT CTATTCGCCA AGCTAGCGGA GAAGGTTCGA 
CCCAGCGAAG CCTCCAAGTT CCGTACCCAT GTCGACGCCG TGGGCAACAT AACCAGTTTA 
CTTCTGTCGC TTTCCGAGCG TTTGGCCCAA ACCGAAAGCA GCCTGGAAAC GCGCCAGCAG 
GAAAGGGGCG CGCTGGAATC AAAGCGGGAT CTGCTGTACG AGCAGATGGA GGAGGCGCAG 
CGTCTCAAAT CGGACATAGA ACGACGTGGA GTCAGCATCG CCGGATTACT GGCCAAGAAC 
CTCAGCGCGG ACATGTGCGC CGACTACGAC TACTTCATCA ACATGAAGGC CAAGCTGATC 
GCCGATGCAC GCGACCTGGC CGTAAGGATC AAGGGCAGCG AGGAGCAGCT TAGCTCCCTC 
AGCGATGCGC TAGTCCAAAG CGATTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610952.1
CDS	164..2173
Translation	MAQWFQWLNFRSNSKASYLPRQSLEKLNNTDPDHGIYKLTLTSNEDLVAHTKPSYGVTGKLPNNLPDVLPLGVKLHQQPKLQPGSPNGDANVTLRYGSNNNLTGNSPTVAPPPYYGGGQRYSTPVLGQGYGKSSKPVTPQQYTRSQSYDVKHTSAVTMPTMSQSHVDLKQAAHDLETTLEEVLPTATPTPTPTPTPTPPRLSPASSHSDCSLSTSSLECTINPIATPIPKPEAHIFRAEVISTTLNTNPLTTPPKPAMNRQESLRENIEKITQLQSVLMSAHLCDASLLGGYTTPLITSPTASFANEPLMTPPLPPSPPPPLEPEEEEEQEENDVHDKQPEIEELQLMQRSELVLMVNPKPSTTDMACQTDELEDRDTDLEAAREEHQTRTTLQPRQRQPIELDYEQMSRELVKLLPPGDKIADILTPKICKPTSQYVSNLYNPDVPLRLAKRDVGTSTLMRMKSITSSAEIRVVSVELQLAEPSEEPTNLIKQKMDELIKHLNQKIVSLKREQQTISEECSANDRLGQDLFAKLAEKVRPSEASKFRTHVDAVGNITSLLLSLSERLAQTESSLETRQQERGALESKRDLLYEQMEEAQRLKSDIERRGVSIAGLLAKNLSADMCADYDYFINMKAKLIADARDLAVRIKGSEEQLSSLSDALVQSDC

Target ORF information:

RefSeq Version	NM_137108.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shroom, transcript variant F (Shrm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137108.3

ORF Insert Sequence:

ATGGCTCAGT GGTTTCAATG GCTCAACTTT CGATCGAATT CCAAGGCCTC ATACTTGCCG 
CGTCAGAGTC TGGAGAAGTT GAACAACACT GATCCCGACC ATGGCATATA CAAGCTCACC 
CTGACCTCCA ACGAGGACTT GGTGGCCCAC ACGAAGCCCA GCTATGGGGT CACAGGAAAG 
CTGCCCAACA ATCTGCCGGA TGTCCTGCCG CTGGGCGTTA AGCTCCACCA GCAGCCAAAG 
TTGCAGCCAG GATCGCCGAA CGGCGATGCG AATGTGACCC TGCGCTATGG CTCCAACAAC 
AATCTGACTG GGAATTCCCC GACGGTTGCC CCGCCCCCCT ACTATGGGGG CGGCCAGCGG 
TATTCAACTC CTGTGCTGGG TCAAGGTTAC GGCAAAAGTT CGAAGCCCGT GACCCCGCAA 
CAATATACGA GATCTCAGTC GTACGATGTG AAGCACACTA GTGCGGTGAC TATGCCGACA 
ATGTCCCAGT CCCACGTGGA TCTCAAGCAG GCCGCCCATG ACCTAGAGAC GACGCTGGAG 
GAGGTGCTGC CCACTGCCAC GCCCACGCCG ACGCCAACAC CGACGCCCAC ACCGCCACGC 
CTCTCGCCGG CTTCCTCGCA CTCGGACTGC AGTCTGAGCA CCAGTTCCTT GGAGTGCACA 
ATCAATCCTA TAGCGACACC GATTCCTAAG CCTGAGGCGC ACATCTTTCG CGCCGAGGTG 
ATTAGCACCA CCCTGAACAC AAATCCGTTG ACAACACCGC CCAAGCCCGC GATGAACCGC 
CAGGAATCCC TGAGGGAGAA CATCGAAAAG ATCACCCAAC TACAGTCGGT GCTGATGTCG 
GCGCACCTGT GTGATGCGAG TCTACTAGGT GGTTACACCA CTCCACTGAT AACCAGTCCC 
ACTGCCAGTT TCGCTAACGA ACCACTAATG ACACCACCAC TGCCGCCCAG TCCGCCACCG 
CCACTAGAAC CGGAGGAGGA GGAGGAGCAG GAGGAGAACG ATGTGCACGA CAAGCAGCCA 
GAGATCGAGG AACTGCAGCT GATGCAGCGC AGCGAATTGG TCCTAATGGT GAATCCCAAG 
CCGAGCACAA CGGATATGGC CTGCCAAACG GACGAGCTGG AGGACAGGGA CACGGACCTC 
GAAGCGGCAC GCGAGGAGCA CCAGACTAGA ACGACTCTGC AGCCGCGACA GCGCCAGCCC 
ATCGAGCTGG ACTACGAGCA GATGAGCCGG GAGCTGGTTA AGCTCCTACC GCCTGGTGAC 
AAGATCGCCG ACATCCTCAC ACCAAAGATC TGCAAGCCCA CCTCGCAATA CGTTAGCAAT 
CTGTACAATC CGGATGTGCC ACTGCGCTTG GCCAAGCGCG ATGTTGGCAC CTCTACGTTG 
ATGCGAATGA AGTCCATCAC GTCGTCTGCC GAGATCCGAG TGGTCAGTGT GGAGCTGCAG 
CTGGCAGAGC CGAGCGAGGA GCCGACGAAT TTAATCAAGC AAAAGATGGA TGAGCTCATC 
AAGCATTTGA ACCAAAAAAT TGTCTCCCTG AAACGCGAGC AGCAGACGAT CAGCGAGGAG 
TGCTCGGCCA ATGACAGACT GGGCCAGGAT CTATTCGCCA AGCTAGCGGA GAAGGTTCGA 
CCCAGCGAAG CCTCCAAGTT CCGTACCCAT GTCGACGCCG TGGGCAACAT AACCAGTTTA 
CTTCTGTCGC TTTCCGAGCG TTTGGCCCAA ACCGAAAGCA GCCTGGAAAC GCGCCAGCAG 
GAAAGGGGCG CGCTGGAATC AAAGCGGGAT CTGCTGTACG AGCAGATGGA GGAGGCGCAG 
CGTCTCAAAT CGGACATAGA ACGACGTGGA GTCAGCATCG CCGGATTACT GGCCAAGAAC 
CTCAGCGCGG ACATGTGCGC CGACTACGAC TACTTCATCA ACATGAAGGC CAAGCTGATC 
GCCGATGCAC GCGACCTGGC CGTAAGGATC AAGGGCAGCG AGGAGCAGCT TAGCTCCCTC 
AGCGATGCGC TAGTCCAAAG CGATTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shrm ( NM_001103837.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097307.1 Drosophila melanogaster shroom, transcript variant D (Shrm), mRNA.

Price & Availability↑

CloneID	OFa09967
Clone ID Related Accession (Same CDS sequence)	NM_001103837.2 , NM_001299488.1
Accession Version	NM_001103837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shroom, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103837.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGC GCAATCACAA GGAGAACGGA AACGGCTCCG AAATGGGCGA GTCGACGAAG 
AGCTTGGCCA AAATGGAGCC GGAGAACAAC AATAAGATTT CGGTGGTGTC CGTATCGAAG 
CTCCTGCTCA AGGATAGCAA TGGGGCTAAC AGCCGGAGTA GCAACAGCAA TGCCAGCTTC 
AGTTCCGCAT CGGTCGCCGG TTCTGTACAG GATGACCTGC CGCACCACAA CAGCAGCAGC 
AGCCAGCTGG GCCAGCAACA CGGCTCATCG TTGGACCAGT GCGGATTGAC TCAGGCCGGC 
CTCGAGGAGT ACAATAATAG ATCGTCCTCG TACTACGACC AGACGGCCTT CCATCACCAG 
AAGCAGCCAT CCTATGCCCA ATCCGAGGGC TACCACAGCT ATGTGTCAAG TTCGGATTCC 
ACATCGGCCA CGCCATTTCT GGATAAATTA CGTCAGGAGA GCGATCTGCT GTCGCGCCAA 
TCGCATCATT GGTCGGAGAA CGATCTGTCC TCCGTTTGCA GCAACTCTGT GGCGCCTTCG 
CCCATTCCGC TGTTGGCCCG TCAGTCTCAC TCCCACTCTC ATTCTCACGC GCATTCCCAT 
TCGAACTCCC ATGGCCATTC CCACGGTCAC GCCCACTCAG CCTCCTCATC CTCATCCAGC 
AACAACAATA GCAACGGCAG CGCCACCAAC AACAACAACA ACAACAGCTC GGAAAGCACT 
TCCTCCACGG AAACCCTCAA GTGGCTGGGC TCCATGAGCG ATATATCCGA AGCCAGTCAT 
GCAACCGGCT ACAGCGCCAT CTCCGAATCG GTTTCCTCCT CGCAGCGCAT TGTCCACAGT 
TCCCGGGTGC CGACACCCAA GCGTCATCAT AGCGAGAGCG TGCTGTATCT GCACAACAAC 
GAGGAGCAAG GCGACAGCTC GCCCACTGCG AGCAACTCCT CGCAGATGAT GATCTCCGAG 
GAGGCGAATG GCGAGGAATC GCCGCCGTCG GTGCAGCCAC TTCGCATCCA GCACCGTCAC 
AGTCCCAGCT ATCCGCCCGT GCACACCTCG ATGGTGCTGC ACCACTTTCA GCAGCAGCAG 
CAGCAGCAGC AGGATTACCA GCACCCGAGT CGCCACCACA CCAACCAGTC CACGTTGAGC 
ACACAAAGTT CCCTGCTGGA GCTGGCCTCG CCCACGGAGA AACCTCGCTC CCTCATGGGA 
CAATCCCACT CCATGGGCGA CCTGCAGCAA AAGAATCCGC ATCAGAATCC GATGTTGGGA 
CGATCGGCTG GTCAGCAGCA CAAGTCCAGC ATTTCCGTGA CCATTTCCAG CAGCGAGGCC 
GTGGTCACCA TTGCACCACA ACCGCCAGCT GGTAAGCCCA GCAAGCTGCA GTTGTCCCTG 
GGAAAGTCGG AGGCCCTCAG TTGCAGTACA CCCAATATGG GGGAGCAGAG TCCCACGAAC 
AGCATCGATT CCTACCGCAG CAACCATCGC CTGTTCCCGG TGAGCACCTA CACGGAGCCG 
GTGCACAGCA ACACCTCGCA GTACGTGCAG CATCCCAAGC CGCAGTTCAG CTCCGGGCTG 
CACAAGTCCG CCAAACTTCC TGTGATAACG CCAGCGGGGG CCACAGTGCA GCCCACCTGG 
CACTCGGTGG CCGAGAGGAT TAACGACTTT GAGCGCAGTC AGTTGGGGGA GCCACCGAAG 
TTTGCCTACC TGGAGCCCAC CAAGACGCAC CGCCTCTCGA ATCCGGCTCT AAAGGCTCTC 
CAGAAGAACG CAGTGCAGTC CTATGTGGAA CGACAGCAGC AGCAGCAGAA GGAGGAACAG 
CAGCTACTAC GTCCGCACTC GCAATCCTAC CAAGCGTGTC ATGTGGAGCG CAAATCACTG 
CCGAACAACT TGAGTCCCAT AATGGTGGGT CTGCCCACTG GGAGTAACTC CGCATCGACT 
CGGGACTGCA GTTCACCCAC TCCACCACCA CCGCCACGAC GTTCGGGGAG TCTGCTGCCC 
AATCTGCTAA GGCGCTCCAG TTCGGCCTCG GACTACGCGG AGTTCAGGGA GCTGCATCAG 
GCACAGGGTC AGGTCAAGGG ACCGAGCATT AGGAACATAA GCAATGCCGA GAAAATCTCC 
TTCAATGACT GCGGAATGCC ACCTCCGCCG CCGCCACCAC GAGGACGTTT GGCCGTGCCG 
ACCAGACGCA CATCCTCGGC AACGGAATAC GCACCCATGC GGGACAAACT GCTGTTGCAG 
CAGGCCGCCG CCTTGGCCCA CCAGCAGCAC CACCCGCAGC AGCATCGCCA TGCCCAACCG 
CCCCATGTGC CGCCCGAGCG TCCGCCCAAG CATCCCAATC TTCGGGTGCC GTCGCCTGAG 
CTGCCACCGC CGCCGCAGAG TGAACTTGAC ATCAGTTATA CCTTCGATGA GCCATTGCCG 
CCGCCACCGC CGCCGGAAGT GCTCCAGCCA CGCCCACCGC CCTCGCCCAA CCGGCGGAAT 
TGCTTCGCCG GAGCATCCAC ACGTCGCACC ACCTACGAAG CACCACCGCC CACCGCAATT 
GTCGCCGCCA AGGTGCCACC GCTGGTGCCC AAGAAGCCAA CGAGCTTGCA GCACAAGCAT 
CTCGCCAACG GAGGAGGCGG CAGTCGCAAG CGCCCGCACC ACGCGACTCC ACAGCCCATC 
CTCGAAAATG TGGCCAGTCC CGTGGCGCCA CCGCCGCCCC TGTTGCCGCG TGCCAGATCC 
ACCGCCCATG ACAATGTGAT TGCCAGCAAT CTGGAGAGCA ACCAGCAGAA ACGATCGAAT 
TCCAAGGCCT CATACTTGCC GCGTCAGAGT CTGGAGAAGT TGAACAACAC TGATCCCGAC 
CATGGCATAT ACAAGCTCAC CCTGACCTCC AACGAGGACT TGGTGGCCCA CACGAAGCCC 
AGCTATGGGG TCACAGGAAA GCTGCCCAAC AATCTGCCGG ATGTCCTGCC GCTGGGCGTT 
AAGCTCCACC AGCAGCCAAA GTTGCAGCCA GGATCGCCGA ACGGCGATGC GAATGTGACC 
CTGCGCTATG GCTCCAACAA CAATCTGACT GGGAATTCCC CGACGGTTGC CCCGCCCCCC 
TACTATGGGG GCGGCCAGCG GTATTCAACT CCTGTGCTGG GTCAAGGTTA CGGCAAAAGT 
TCGAAGCCCG TGACCCCGCA ACAATATACG AGATCTCAGT CGTACGATGT GAAGCACACT 
AGTGCGGTGA CTATGCCGAC AATGTCCCAG TCCCACGTGG ATCTCAAGCA GGCCGCCCAT 
GACCTAGAGA CGACGCTGGA GGAGGTGCTG CCCACTGCCA CGCCCACGCC GACGCCAACA 
CCGACGCCCA CACCGCCACG CCTCTCGCCG GCTTCCTCGC ACTCGGACTG CAGTCTGAGC 
ACCAGTTCCT TGGAGTGCAC AATCAATCCT ATAGCGACAC CGATTCCTAA GCCTGAGGCG 
CACATCTTTC GCGCCGAGGT GATTAGCACC ACCCTGAACA CAAATCCGTT GACAACACCG 
CCCAAGCCCG CGATGAACCG CCAGGAATCC CTGAGGGAGA ACATCGAAAA GATCACCCAA 
CTACAGTCGG TGCTGATGTC GGCGCACCTG TGTGATGCGA GTCTACTAGG TGGTTACACC 
ACTCCACTGA TAACCAGTCC CACTGCCAGT TTCGCTAACG AACCACTAAT GACACCACCA 
CTGCCGCCCA GTCCGCCACC GCCACTAGAA CCGGAGGAGG AGGAGGAGCA GGAGGAGAAC 
GATGTGCACG ACAAGCAGCC AGAGATCGAG GAACTGCAGC TGATGCAGCG CAGCGAATTG 
GTCCTAATGG TGAATCCCAA GCCGAGCACA ACGGATATGG CCTGCCAAAC GGACGAGCTG 
GAGGACAGGG ACACGGACCT CGAAGCGGCA CGCGAGGAGC ACCAGACTAG AACGACTCTG 
CAGCCGCGAC AGCGCCAGCC CATCGAGCTG GACTACGAGC AGATGAGCCG GGAGCTGGTT 
AAGCTCCTAC CGCCTGGTGA CAAGATCGCC GACATCCTCA CACCAAAGAT CTGCAAGCCC 
ACCTCGCAAT ACGTTAGCAA TCTGTACAAT CCGGATGTGC CACTGCGCTT GGCCAAGCGC 
GATGTTGGCA CCTCTACGTT GATGCGAATG AAGTCCATCA CGTCGTCTGC CGAGATCCGA 
GTGGTCAGTG TGGAGCTGCA GCTGGCAGAG CCGAGCGAGG AGCCGACGAA TTTAATCAAG 
CAAAAGATGG ATGAGCTCAT CAAGCATTTG AACCAAAAAA TTGTCTCCCT GAAACGCGAG 
CAGCAGACGA TCAGCGAGGA GTGCTCGGCC AATGACAGAC TGGGCCAGGA TCTATTCGCC 
AAGCTAGCGG AGAAGGTTCG ACCCAGCGAA GCCTCCAAGT TCCGTACCCA TGTCGACGCC 
GTGGGCAACA TAACCAGTTT ACTTCTGTCG CTTTCCGAGC GTTTGGCCCA AACCGAAAGC 
AGCCTGGAAA CGCGCCAGCA GGAAAGGGGC GCGCTGGAAT CAAAGCGGGA TCTGCTGTAC 
GAGCAGATGG AGGAGGCGCA GCGTCTCAAA TCGGACATAG AACGACGTGG AGTCAGCATC 
GCCGGATTAC TGGCCAAGAA CCTCAGCGCG GACATGTGCG CCGACTACGA CTACTTCATC 
AACATGAAGG CCAAGCTGAT CGCCGATGCA CGCGACCTGG CCGTAAGGAT CAAGGGCAGC 
GAGGAGCAGC TTAGCTCCCT CAGCGATGCG CTAGTCCAAA GCGATTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097307.1
CDS	240..4970
Translation	MKMRNHKENGNGSEMGESTKSLAKMEPENNNKISVVSVSKLLLKDSNGANSRSSNSNASFSSASVAGSVQDDLPHHNSSSSQLGQQHGSSLDQCGLTQAGLEEYNNRSSSYYDQTAFHHQKQPSYAQSEGYHSYVSSSDSTSATPFLDKLRQESDLLSRQSHHWSENDLSSVCSNSVAPSPIPLLARQSHSHSHSHAHSHSNSHGHSHGHAHSASSSSSSNNNSNGSATNNNNNNSSESTSSTETLKWLGSMSDISEASHATGYSAISESVSSSQRIVHSSRVPTPKRHHSESVLYLHNNEEQGDSSPTASNSSQMMISEEANGEESPPSVQPLRIQHRHSPSYPPVHTSMVLHHFQQQQQQQQDYQHPSRHHTNQSTLSTQSSLLELASPTEKPRSLMGQSHSMGDLQQKNPHQNPMLGRSAGQQHKSSISVTISSSEAVVTIAPQPPAGKPSKLQLSLGKSEALSCSTPNMGEQSPTNSIDSYRSNHRLFPVSTYTEPVHSNTSQYVQHPKPQFSSGLHKSAKLPVITPAGATVQPTWHSVAERINDFERSQLGEPPKFAYLEPTKTHRLSNPALKALQKNAVQSYVERQQQQQKEEQQLLRPHSQSYQACHVERKSLPNNLSPIMVGLPTGSNSASTRDCSSPTPPPPPRRSGSLLPNLLRRSSSASDYAEFRELHQAQGQVKGPSIRNISNAEKISFNDCGMPPPPPPPRGRLAVPTRRTSSATEYAPMRDKLLLQQAAALAHQQHHPQQHRHAQPPHVPPERPPKHPNLRVPSPELPPPPQSELDISYTFDEPLPPPPPPEVLQPRPPPSPNRRNCFAGASTRRTTYEAPPPTAIVAAKVPPLVPKKPTSLQHKHLANGGGGSRKRPHHATPQPILENVASPVAPPPPLLPRARSTAHDNVIASNLESNQQKRSNSKASYLPRQSLEKLNNTDPDHGIYKLTLTSNEDLVAHTKPSYGVTGKLPNNLPDVLPLGVKLHQQPKLQPGSPNGDANVTLRYGSNNNLTGNSPTVAPPPYYGGGQRYSTPVLGQGYGKSSKPVTPQQYTRSQSYDVKHTSAVTMPTMSQSHVDLKQAAHDLETTLEEVLPTATPTPTPTPTPTPPRLSPASSHSDCSLSTSSLECTINPIATPIPKPEAHIFRAEVISTTLNTNPLTTPPKPAMNRQESLRENIEKITQLQSVLMSAHLCDASLLGGYTTPLITSPTASFANEPLMTPPLPPSPPPPLEPEEEEEQEENDVHDKQPEIEELQLMQRSELVLMVNPKPSTTDMACQTDELEDRDTDLEAAREEHQTRTTLQPRQRQPIELDYEQMSRELVKLLPPGDKIADILTPKICKPTSQYVSNLYNPDVPLRLAKRDVGTSTLMRMKSITSSAEIRVVSVELQLAEPSEEPTNLIKQKMDELIKHLNQKIVSLKREQQTISEECSANDRLGQDLFAKLAEKVRPSEASKFRTHVDAVGNITSLLLSLSERLAQTESSLETRQQERGALESKRDLLYEQMEEAQRLKSDIERRGVSIAGLLAKNLSADMCADYDYFINMKAKLIADARDLAVRIKGSEEQLSSLSDALVQSDC

Target ORF information:

RefSeq Version	NM_001103837.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shroom, transcript variant D (Shrm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103837.2

ORF Insert Sequence:

ATGAAAATGC GCAATCACAA GGAGAACGGA AACGGCTCCG AAATGGGCGA GTCGACGAAG 
AGCTTGGCCA AAATGGAGCC GGAGAACAAC AATAAGATTT CGGTGGTGTC CGTATCGAAG 
CTCCTGCTCA AGGATAGCAA TGGGGCTAAC AGCCGGAGTA GCAACAGCAA TGCCAGCTTC 
AGTTCCGCAT CGGTCGCCGG TTCTGTACAG GATGACCTGC CGCACCACAA CAGCAGCAGC 
AGCCAGCTGG GCCAGCAACA CGGCTCATCG TTGGACCAGT GCGGATTGAC TCAGGCCGGC 
CTCGAGGAGT ACAATAATAG ATCGTCCTCG TACTACGACC AGACGGCCTT CCATCACCAG 
AAGCAGCCAT CCTATGCCCA ATCCGAGGGC TACCACAGCT ATGTGTCAAG TTCGGATTCC 
ACATCGGCCA CGCCATTTCT GGATAAATTA CGTCAGGAGA GCGATCTGCT GTCGCGCCAA 
TCGCATCATT GGTCGGAGAA CGATCTGTCC TCCGTTTGCA GCAACTCTGT GGCGCCTTCG 
CCCATTCCGC TGTTGGCCCG TCAGTCTCAC TCCCACTCTC ATTCTCACGC GCATTCCCAT 
TCGAACTCCC ATGGCCATTC CCACGGTCAC GCCCACTCAG CCTCCTCATC CTCATCCAGC 
AACAACAATA GCAACGGCAG CGCCACCAAC AACAACAACA ACAACAGCTC GGAAAGCACT 
TCCTCCACGG AAACCCTCAA GTGGCTGGGC TCCATGAGCG ATATATCCGA AGCCAGTCAT 
GCAACCGGCT ACAGCGCCAT CTCCGAATCG GTTTCCTCCT CGCAGCGCAT TGTCCACAGT 
TCCCGGGTGC CGACACCCAA GCGTCATCAT AGCGAGAGCG TGCTGTATCT GCACAACAAC 
GAGGAGCAAG GCGACAGCTC GCCCACTGCG AGCAACTCCT CGCAGATGAT GATCTCCGAG 
GAGGCGAATG GCGAGGAATC GCCGCCGTCG GTGCAGCCAC TTCGCATCCA GCACCGTCAC 
AGTCCCAGCT ATCCGCCCGT GCACACCTCG ATGGTGCTGC ACCACTTTCA GCAGCAGCAG 
CAGCAGCAGC AGGATTACCA GCACCCGAGT CGCCACCACA CCAACCAGTC CACGTTGAGC 
ACACAAAGTT CCCTGCTGGA GCTGGCCTCG CCCACGGAGA AACCTCGCTC CCTCATGGGA 
CAATCCCACT CCATGGGCGA CCTGCAGCAA AAGAATCCGC ATCAGAATCC GATGTTGGGA 
CGATCGGCTG GTCAGCAGCA CAAGTCCAGC ATTTCCGTGA CCATTTCCAG CAGCGAGGCC 
GTGGTCACCA TTGCACCACA ACCGCCAGCT GGTAAGCCCA GCAAGCTGCA GTTGTCCCTG 
GGAAAGTCGG AGGCCCTCAG TTGCAGTACA CCCAATATGG GGGAGCAGAG TCCCACGAAC 
AGCATCGATT CCTACCGCAG CAACCATCGC CTGTTCCCGG TGAGCACCTA CACGGAGCCG 
GTGCACAGCA ACACCTCGCA GTACGTGCAG CATCCCAAGC CGCAGTTCAG CTCCGGGCTG 
CACAAGTCCG CCAAACTTCC TGTGATAACG CCAGCGGGGG CCACAGTGCA GCCCACCTGG 
CACTCGGTGG CCGAGAGGAT TAACGACTTT GAGCGCAGTC AGTTGGGGGA GCCACCGAAG 
TTTGCCTACC TGGAGCCCAC CAAGACGCAC CGCCTCTCGA ATCCGGCTCT AAAGGCTCTC 
CAGAAGAACG CAGTGCAGTC CTATGTGGAA CGACAGCAGC AGCAGCAGAA GGAGGAACAG 
CAGCTACTAC GTCCGCACTC GCAATCCTAC CAAGCGTGTC ATGTGGAGCG CAAATCACTG 
CCGAACAACT TGAGTCCCAT AATGGTGGGT CTGCCCACTG GGAGTAACTC CGCATCGACT 
CGGGACTGCA GTTCACCCAC TCCACCACCA CCGCCACGAC GTTCGGGGAG TCTGCTGCCC 
AATCTGCTAA GGCGCTCCAG TTCGGCCTCG GACTACGCGG AGTTCAGGGA GCTGCATCAG 
GCACAGGGTC AGGTCAAGGG ACCGAGCATT AGGAACATAA GCAATGCCGA GAAAATCTCC 
TTCAATGACT GCGGAATGCC ACCTCCGCCG CCGCCACCAC GAGGACGTTT GGCCGTGCCG 
ACCAGACGCA CATCCTCGGC AACGGAATAC GCACCCATGC GGGACAAACT GCTGTTGCAG 
CAGGCCGCCG CCTTGGCCCA CCAGCAGCAC CACCCGCAGC AGCATCGCCA TGCCCAACCG 
CCCCATGTGC CGCCCGAGCG TCCGCCCAAG CATCCCAATC TTCGGGTGCC GTCGCCTGAG 
CTGCCACCGC CGCCGCAGAG TGAACTTGAC ATCAGTTATA CCTTCGATGA GCCATTGCCG 
CCGCCACCGC CGCCGGAAGT GCTCCAGCCA CGCCCACCGC CCTCGCCCAA CCGGCGGAAT 
TGCTTCGCCG GAGCATCCAC ACGTCGCACC ACCTACGAAG CACCACCGCC CACCGCAATT 
GTCGCCGCCA AGGTGCCACC GCTGGTGCCC AAGAAGCCAA CGAGCTTGCA GCACAAGCAT 
CTCGCCAACG GAGGAGGCGG CAGTCGCAAG CGCCCGCACC ACGCGACTCC ACAGCCCATC 
CTCGAAAATG TGGCCAGTCC CGTGGCGCCA CCGCCGCCCC TGTTGCCGCG TGCCAGATCC 
ACCGCCCATG ACAATGTGAT TGCCAGCAAT CTGGAGAGCA ACCAGCAGAA ACGATCGAAT 
TCCAAGGCCT CATACTTGCC GCGTCAGAGT CTGGAGAAGT TGAACAACAC TGATCCCGAC 
CATGGCATAT ACAAGCTCAC CCTGACCTCC AACGAGGACT TGGTGGCCCA CACGAAGCCC 
AGCTATGGGG TCACAGGAAA GCTGCCCAAC AATCTGCCGG ATGTCCTGCC GCTGGGCGTT 
AAGCTCCACC AGCAGCCAAA GTTGCAGCCA GGATCGCCGA ACGGCGATGC GAATGTGACC 
CTGCGCTATG GCTCCAACAA CAATCTGACT GGGAATTCCC CGACGGTTGC CCCGCCCCCC 
TACTATGGGG GCGGCCAGCG GTATTCAACT CCTGTGCTGG GTCAAGGTTA CGGCAAAAGT 
TCGAAGCCCG TGACCCCGCA ACAATATACG AGATCTCAGT CGTACGATGT GAAGCACACT 
AGTGCGGTGA CTATGCCGAC AATGTCCCAG TCCCACGTGG ATCTCAAGCA GGCCGCCCAT 
GACCTAGAGA CGACGCTGGA GGAGGTGCTG CCCACTGCCA CGCCCACGCC GACGCCAACA 
CCGACGCCCA CACCGCCACG CCTCTCGCCG GCTTCCTCGC ACTCGGACTG CAGTCTGAGC 
ACCAGTTCCT TGGAGTGCAC AATCAATCCT ATAGCGACAC CGATTCCTAA GCCTGAGGCG 
CACATCTTTC GCGCCGAGGT GATTAGCACC ACCCTGAACA CAAATCCGTT GACAACACCG 
CCCAAGCCCG CGATGAACCG CCAGGAATCC CTGAGGGAGA ACATCGAAAA GATCACCCAA 
CTACAGTCGG TGCTGATGTC GGCGCACCTG TGTGATGCGA GTCTACTAGG TGGTTACACC 
ACTCCACTGA TAACCAGTCC CACTGCCAGT TTCGCTAACG AACCACTAAT GACACCACCA 
CTGCCGCCCA GTCCGCCACC GCCACTAGAA CCGGAGGAGG AGGAGGAGCA GGAGGAGAAC 
GATGTGCACG ACAAGCAGCC AGAGATCGAG GAACTGCAGC TGATGCAGCG CAGCGAATTG 
GTCCTAATGG TGAATCCCAA GCCGAGCACA ACGGATATGG CCTGCCAAAC GGACGAGCTG 
GAGGACAGGG ACACGGACCT CGAAGCGGCA CGCGAGGAGC ACCAGACTAG AACGACTCTG 
CAGCCGCGAC AGCGCCAGCC CATCGAGCTG GACTACGAGC AGATGAGCCG GGAGCTGGTT 
AAGCTCCTAC CGCCTGGTGA CAAGATCGCC GACATCCTCA CACCAAAGAT CTGCAAGCCC 
ACCTCGCAAT ACGTTAGCAA TCTGTACAAT CCGGATGTGC CACTGCGCTT GGCCAAGCGC 
GATGTTGGCA CCTCTACGTT GATGCGAATG AAGTCCATCA CGTCGTCTGC CGAGATCCGA 
GTGGTCAGTG TGGAGCTGCA GCTGGCAGAG CCGAGCGAGG AGCCGACGAA TTTAATCAAG 
CAAAAGATGG ATGAGCTCAT CAAGCATTTG AACCAAAAAA TTGTCTCCCT GAAACGCGAG 
CAGCAGACGA TCAGCGAGGA GTGCTCGGCC AATGACAGAC TGGGCCAGGA TCTATTCGCC 
AAGCTAGCGG AGAAGGTTCG ACCCAGCGAA GCCTCCAAGT TCCGTACCCA TGTCGACGCC 
GTGGGCAACA TAACCAGTTT ACTTCTGTCG CTTTCCGAGC GTTTGGCCCA AACCGAAAGC 
AGCCTGGAAA CGCGCCAGCA GGAAAGGGGC GCGCTGGAAT CAAAGCGGGA TCTGCTGTAC 
GAGCAGATGG AGGAGGCGCA GCGTCTCAAA TCGGACATAG AACGACGTGG AGTCAGCATC 
GCCGGATTAC TGGCCAAGAA CCTCAGCGCG GACATGTGCG CCGACTACGA CTACTTCATC 
AACATGAAGG CCAAGCTGAT CGCCGATGCA CGCGACCTGG CCGTAAGGAT CAAGGGCAGC 
GAGGAGCAGC TTAGCTCCCT CAGCGATGCG CTAGTCCAAA GCGATTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shrm ( NM_001299488.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286417.1 Drosophila melanogaster shroom, transcript variant H (Shrm), mRNA.

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CloneID	OFa09967
Clone ID Related Accession (Same CDS sequence)	NM_001103837.2 , NM_001299488.1
Accession Version	NM_001299488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shroom, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGC GCAATCACAA GGAGAACGGA AACGGCTCCG AAATGGGCGA GTCGACGAAG 
AGCTTGGCCA AAATGGAGCC GGAGAACAAC AATAAGATTT CGGTGGTGTC CGTATCGAAG 
CTCCTGCTCA AGGATAGCAA TGGGGCTAAC AGCCGGAGTA GCAACAGCAA TGCCAGCTTC 
AGTTCCGCAT CGGTCGCCGG TTCTGTACAG GATGACCTGC CGCACCACAA CAGCAGCAGC 
AGCCAGCTGG GCCAGCAACA CGGCTCATCG TTGGACCAGT GCGGATTGAC TCAGGCCGGC 
CTCGAGGAGT ACAATAATAG ATCGTCCTCG TACTACGACC AGACGGCCTT CCATCACCAG 
AAGCAGCCAT CCTATGCCCA ATCCGAGGGC TACCACAGCT ATGTGTCAAG TTCGGATTCC 
ACATCGGCCA CGCCATTTCT GGATAAATTA CGTCAGGAGA GCGATCTGCT GTCGCGCCAA 
TCGCATCATT GGTCGGAGAA CGATCTGTCC TCCGTTTGCA GCAACTCTGT GGCGCCTTCG 
CCCATTCCGC TGTTGGCCCG TCAGTCTCAC TCCCACTCTC ATTCTCACGC GCATTCCCAT 
TCGAACTCCC ATGGCCATTC CCACGGTCAC GCCCACTCAG CCTCCTCATC CTCATCCAGC 
AACAACAATA GCAACGGCAG CGCCACCAAC AACAACAACA ACAACAGCTC GGAAAGCACT 
TCCTCCACGG AAACCCTCAA GTGGCTGGGC TCCATGAGCG ATATATCCGA AGCCAGTCAT 
GCAACCGGCT ACAGCGCCAT CTCCGAATCG GTTTCCTCCT CGCAGCGCAT TGTCCACAGT 
TCCCGGGTGC CGACACCCAA GCGTCATCAT AGCGAGAGCG TGCTGTATCT GCACAACAAC 
GAGGAGCAAG GCGACAGCTC GCCCACTGCG AGCAACTCCT CGCAGATGAT GATCTCCGAG 
GAGGCGAATG GCGAGGAATC GCCGCCGTCG GTGCAGCCAC TTCGCATCCA GCACCGTCAC 
AGTCCCAGCT ATCCGCCCGT GCACACCTCG ATGGTGCTGC ACCACTTTCA GCAGCAGCAG 
CAGCAGCAGC AGGATTACCA GCACCCGAGT CGCCACCACA CCAACCAGTC CACGTTGAGC 
ACACAAAGTT CCCTGCTGGA GCTGGCCTCG CCCACGGAGA AACCTCGCTC CCTCATGGGA 
CAATCCCACT CCATGGGCGA CCTGCAGCAA AAGAATCCGC ATCAGAATCC GATGTTGGGA 
CGATCGGCTG GTCAGCAGCA CAAGTCCAGC ATTTCCGTGA CCATTTCCAG CAGCGAGGCC 
GTGGTCACCA TTGCACCACA ACCGCCAGCT GGTAAGCCCA GCAAGCTGCA GTTGTCCCTG 
GGAAAGTCGG AGGCCCTCAG TTGCAGTACA CCCAATATGG GGGAGCAGAG TCCCACGAAC 
AGCATCGATT CCTACCGCAG CAACCATCGC CTGTTCCCGG TGAGCACCTA CACGGAGCCG 
GTGCACAGCA ACACCTCGCA GTACGTGCAG CATCCCAAGC CGCAGTTCAG CTCCGGGCTG 
CACAAGTCCG CCAAACTTCC TGTGATAACG CCAGCGGGGG CCACAGTGCA GCCCACCTGG 
CACTCGGTGG CCGAGAGGAT TAACGACTTT GAGCGCAGTC AGTTGGGGGA GCCACCGAAG 
TTTGCCTACC TGGAGCCCAC CAAGACGCAC CGCCTCTCGA ATCCGGCTCT AAAGGCTCTC 
CAGAAGAACG CAGTGCAGTC CTATGTGGAA CGACAGCAGC AGCAGCAGAA GGAGGAACAG 
CAGCTACTAC GTCCGCACTC GCAATCCTAC CAAGCGTGTC ATGTGGAGCG CAAATCACTG 
CCGAACAACT TGAGTCCCAT AATGGTGGGT CTGCCCACTG GGAGTAACTC CGCATCGACT 
CGGGACTGCA GTTCACCCAC TCCACCACCA CCGCCACGAC GTTCGGGGAG TCTGCTGCCC 
AATCTGCTAA GGCGCTCCAG TTCGGCCTCG GACTACGCGG AGTTCAGGGA GCTGCATCAG 
GCACAGGGTC AGGTCAAGGG ACCGAGCATT AGGAACATAA GCAATGCCGA GAAAATCTCC 
TTCAATGACT GCGGAATGCC ACCTCCGCCG CCGCCACCAC GAGGACGTTT GGCCGTGCCG 
ACCAGACGCA CATCCTCGGC AACGGAATAC GCACCCATGC GGGACAAACT GCTGTTGCAG 
CAGGCCGCCG CCTTGGCCCA CCAGCAGCAC CACCCGCAGC AGCATCGCCA TGCCCAACCG 
CCCCATGTGC CGCCCGAGCG TCCGCCCAAG CATCCCAATC TTCGGGTGCC GTCGCCTGAG 
CTGCCACCGC CGCCGCAGAG TGAACTTGAC ATCAGTTATA CCTTCGATGA GCCATTGCCG 
CCGCCACCGC CGCCGGAAGT GCTCCAGCCA CGCCCACCGC CCTCGCCCAA CCGGCGGAAT 
TGCTTCGCCG GAGCATCCAC ACGTCGCACC ACCTACGAAG CACCACCGCC CACCGCAATT 
GTCGCCGCCA AGGTGCCACC GCTGGTGCCC AAGAAGCCAA CGAGCTTGCA GCACAAGCAT 
CTCGCCAACG GAGGAGGCGG CAGTCGCAAG CGCCCGCACC ACGCGACTCC ACAGCCCATC 
CTCGAAAATG TGGCCAGTCC CGTGGCGCCA CCGCCGCCCC TGTTGCCGCG TGCCAGATCC 
ACCGCCCATG ACAATGTGAT TGCCAGCAAT CTGGAGAGCA ACCAGCAGAA ACGATCGAAT 
TCCAAGGCCT CATACTTGCC GCGTCAGAGT CTGGAGAAGT TGAACAACAC TGATCCCGAC 
CATGGCATAT ACAAGCTCAC CCTGACCTCC AACGAGGACT TGGTGGCCCA CACGAAGCCC 
AGCTATGGGG TCACAGGAAA GCTGCCCAAC AATCTGCCGG ATGTCCTGCC GCTGGGCGTT 
AAGCTCCACC AGCAGCCAAA GTTGCAGCCA GGATCGCCGA ACGGCGATGC GAATGTGACC 
CTGCGCTATG GCTCCAACAA CAATCTGACT GGGAATTCCC CGACGGTTGC CCCGCCCCCC 
TACTATGGGG GCGGCCAGCG GTATTCAACT CCTGTGCTGG GTCAAGGTTA CGGCAAAAGT 
TCGAAGCCCG TGACCCCGCA ACAATATACG AGATCTCAGT CGTACGATGT GAAGCACACT 
AGTGCGGTGA CTATGCCGAC AATGTCCCAG TCCCACGTGG ATCTCAAGCA GGCCGCCCAT 
GACCTAGAGA CGACGCTGGA GGAGGTGCTG CCCACTGCCA CGCCCACGCC GACGCCAACA 
CCGACGCCCA CACCGCCACG CCTCTCGCCG GCTTCCTCGC ACTCGGACTG CAGTCTGAGC 
ACCAGTTCCT TGGAGTGCAC AATCAATCCT ATAGCGACAC CGATTCCTAA GCCTGAGGCG 
CACATCTTTC GCGCCGAGGT GATTAGCACC ACCCTGAACA CAAATCCGTT GACAACACCG 
CCCAAGCCCG CGATGAACCG CCAGGAATCC CTGAGGGAGA ACATCGAAAA GATCACCCAA 
CTACAGTCGG TGCTGATGTC GGCGCACCTG TGTGATGCGA GTCTACTAGG TGGTTACACC 
ACTCCACTGA TAACCAGTCC CACTGCCAGT TTCGCTAACG AACCACTAAT GACACCACCA 
CTGCCGCCCA GTCCGCCACC GCCACTAGAA CCGGAGGAGG AGGAGGAGCA GGAGGAGAAC 
GATGTGCACG ACAAGCAGCC AGAGATCGAG GAACTGCAGC TGATGCAGCG CAGCGAATTG 
GTCCTAATGG TGAATCCCAA GCCGAGCACA ACGGATATGG CCTGCCAAAC GGACGAGCTG 
GAGGACAGGG ACACGGACCT CGAAGCGGCA CGCGAGGAGC ACCAGACTAG AACGACTCTG 
CAGCCGCGAC AGCGCCAGCC CATCGAGCTG GACTACGAGC AGATGAGCCG GGAGCTGGTT 
AAGCTCCTAC CGCCTGGTGA CAAGATCGCC GACATCCTCA CACCAAAGAT CTGCAAGCCC 
ACCTCGCAAT ACGTTAGCAA TCTGTACAAT CCGGATGTGC CACTGCGCTT GGCCAAGCGC 
GATGTTGGCA CCTCTACGTT GATGCGAATG AAGTCCATCA CGTCGTCTGC CGAGATCCGA 
GTGGTCAGTG TGGAGCTGCA GCTGGCAGAG CCGAGCGAGG AGCCGACGAA TTTAATCAAG 
CAAAAGATGG ATGAGCTCAT CAAGCATTTG AACCAAAAAA TTGTCTCCCT GAAACGCGAG 
CAGCAGACGA TCAGCGAGGA GTGCTCGGCC AATGACAGAC TGGGCCAGGA TCTATTCGCC 
AAGCTAGCGG AGAAGGTTCG ACCCAGCGAA GCCTCCAAGT TCCGTACCCA TGTCGACGCC 
GTGGGCAACA TAACCAGTTT ACTTCTGTCG CTTTCCGAGC GTTTGGCCCA AACCGAAAGC 
AGCCTGGAAA CGCGCCAGCA GGAAAGGGGC GCGCTGGAAT CAAAGCGGGA TCTGCTGTAC 
GAGCAGATGG AGGAGGCGCA GCGTCTCAAA TCGGACATAG AACGACGTGG AGTCAGCATC 
GCCGGATTAC TGGCCAAGAA CCTCAGCGCG GACATGTGCG CCGACTACGA CTACTTCATC 
AACATGAAGG CCAAGCTGAT CGCCGATGCA CGCGACCTGG CCGTAAGGAT CAAGGGCAGC 
GAGGAGCAGC TTAGCTCCCT CAGCGATGCG CTAGTCCAAA GCGATTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286417.1
CDS	443..5173
Translation	MKMRNHKENGNGSEMGESTKSLAKMEPENNNKISVVSVSKLLLKDSNGANSRSSNSNASFSSASVAGSVQDDLPHHNSSSSQLGQQHGSSLDQCGLTQAGLEEYNNRSSSYYDQTAFHHQKQPSYAQSEGYHSYVSSSDSTSATPFLDKLRQESDLLSRQSHHWSENDLSSVCSNSVAPSPIPLLARQSHSHSHSHAHSHSNSHGHSHGHAHSASSSSSSNNNSNGSATNNNNNNSSESTSSTETLKWLGSMSDISEASHATGYSAISESVSSSQRIVHSSRVPTPKRHHSESVLYLHNNEEQGDSSPTASNSSQMMISEEANGEESPPSVQPLRIQHRHSPSYPPVHTSMVLHHFQQQQQQQQDYQHPSRHHTNQSTLSTQSSLLELASPTEKPRSLMGQSHSMGDLQQKNPHQNPMLGRSAGQQHKSSISVTISSSEAVVTIAPQPPAGKPSKLQLSLGKSEALSCSTPNMGEQSPTNSIDSYRSNHRLFPVSTYTEPVHSNTSQYVQHPKPQFSSGLHKSAKLPVITPAGATVQPTWHSVAERINDFERSQLGEPPKFAYLEPTKTHRLSNPALKALQKNAVQSYVERQQQQQKEEQQLLRPHSQSYQACHVERKSLPNNLSPIMVGLPTGSNSASTRDCSSPTPPPPPRRSGSLLPNLLRRSSSASDYAEFRELHQAQGQVKGPSIRNISNAEKISFNDCGMPPPPPPPRGRLAVPTRRTSSATEYAPMRDKLLLQQAAALAHQQHHPQQHRHAQPPHVPPERPPKHPNLRVPSPELPPPPQSELDISYTFDEPLPPPPPPEVLQPRPPPSPNRRNCFAGASTRRTTYEAPPPTAIVAAKVPPLVPKKPTSLQHKHLANGGGGSRKRPHHATPQPILENVASPVAPPPPLLPRARSTAHDNVIASNLESNQQKRSNSKASYLPRQSLEKLNNTDPDHGIYKLTLTSNEDLVAHTKPSYGVTGKLPNNLPDVLPLGVKLHQQPKLQPGSPNGDANVTLRYGSNNNLTGNSPTVAPPPYYGGGQRYSTPVLGQGYGKSSKPVTPQQYTRSQSYDVKHTSAVTMPTMSQSHVDLKQAAHDLETTLEEVLPTATPTPTPTPTPTPPRLSPASSHSDCSLSTSSLECTINPIATPIPKPEAHIFRAEVISTTLNTNPLTTPPKPAMNRQESLRENIEKITQLQSVLMSAHLCDASLLGGYTTPLITSPTASFANEPLMTPPLPPSPPPPLEPEEEEEQEENDVHDKQPEIEELQLMQRSELVLMVNPKPSTTDMACQTDELEDRDTDLEAAREEHQTRTTLQPRQRQPIELDYEQMSRELVKLLPPGDKIADILTPKICKPTSQYVSNLYNPDVPLRLAKRDVGTSTLMRMKSITSSAEIRVVSVELQLAEPSEEPTNLIKQKMDELIKHLNQKIVSLKREQQTISEECSANDRLGQDLFAKLAEKVRPSEASKFRTHVDAVGNITSLLLSLSERLAQTESSLETRQQERGALESKRDLLYEQMEEAQRLKSDIERRGVSIAGLLAKNLSADMCADYDYFINMKAKLIADARDLAVRIKGSEEQLSSLSDALVQSDC

Target ORF information:

RefSeq Version	NM_001299488.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shroom, transcript variant H (Shrm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299488.1

ORF Insert Sequence:

ATGAAAATGC GCAATCACAA GGAGAACGGA AACGGCTCCG AAATGGGCGA GTCGACGAAG 
AGCTTGGCCA AAATGGAGCC GGAGAACAAC AATAAGATTT CGGTGGTGTC CGTATCGAAG 
CTCCTGCTCA AGGATAGCAA TGGGGCTAAC AGCCGGAGTA GCAACAGCAA TGCCAGCTTC 
AGTTCCGCAT CGGTCGCCGG TTCTGTACAG GATGACCTGC CGCACCACAA CAGCAGCAGC 
AGCCAGCTGG GCCAGCAACA CGGCTCATCG TTGGACCAGT GCGGATTGAC TCAGGCCGGC 
CTCGAGGAGT ACAATAATAG ATCGTCCTCG TACTACGACC AGACGGCCTT CCATCACCAG 
AAGCAGCCAT CCTATGCCCA ATCCGAGGGC TACCACAGCT ATGTGTCAAG TTCGGATTCC 
ACATCGGCCA CGCCATTTCT GGATAAATTA CGTCAGGAGA GCGATCTGCT GTCGCGCCAA 
TCGCATCATT GGTCGGAGAA CGATCTGTCC TCCGTTTGCA GCAACTCTGT GGCGCCTTCG 
CCCATTCCGC TGTTGGCCCG TCAGTCTCAC TCCCACTCTC ATTCTCACGC GCATTCCCAT 
TCGAACTCCC ATGGCCATTC CCACGGTCAC GCCCACTCAG CCTCCTCATC CTCATCCAGC 
AACAACAATA GCAACGGCAG CGCCACCAAC AACAACAACA ACAACAGCTC GGAAAGCACT 
TCCTCCACGG AAACCCTCAA GTGGCTGGGC TCCATGAGCG ATATATCCGA AGCCAGTCAT 
GCAACCGGCT ACAGCGCCAT CTCCGAATCG GTTTCCTCCT CGCAGCGCAT TGTCCACAGT 
TCCCGGGTGC CGACACCCAA GCGTCATCAT AGCGAGAGCG TGCTGTATCT GCACAACAAC 
GAGGAGCAAG GCGACAGCTC GCCCACTGCG AGCAACTCCT CGCAGATGAT GATCTCCGAG 
GAGGCGAATG GCGAGGAATC GCCGCCGTCG GTGCAGCCAC TTCGCATCCA GCACCGTCAC 
AGTCCCAGCT ATCCGCCCGT GCACACCTCG ATGGTGCTGC ACCACTTTCA GCAGCAGCAG 
CAGCAGCAGC AGGATTACCA GCACCCGAGT CGCCACCACA CCAACCAGTC CACGTTGAGC 
ACACAAAGTT CCCTGCTGGA GCTGGCCTCG CCCACGGAGA AACCTCGCTC CCTCATGGGA 
CAATCCCACT CCATGGGCGA CCTGCAGCAA AAGAATCCGC ATCAGAATCC GATGTTGGGA 
CGATCGGCTG GTCAGCAGCA CAAGTCCAGC ATTTCCGTGA CCATTTCCAG CAGCGAGGCC 
GTGGTCACCA TTGCACCACA ACCGCCAGCT GGTAAGCCCA GCAAGCTGCA GTTGTCCCTG 
GGAAAGTCGG AGGCCCTCAG TTGCAGTACA CCCAATATGG GGGAGCAGAG TCCCACGAAC 
AGCATCGATT CCTACCGCAG CAACCATCGC CTGTTCCCGG TGAGCACCTA CACGGAGCCG 
GTGCACAGCA ACACCTCGCA GTACGTGCAG CATCCCAAGC CGCAGTTCAG CTCCGGGCTG 
CACAAGTCCG CCAAACTTCC TGTGATAACG CCAGCGGGGG CCACAGTGCA GCCCACCTGG 
CACTCGGTGG CCGAGAGGAT TAACGACTTT GAGCGCAGTC AGTTGGGGGA GCCACCGAAG 
TTTGCCTACC TGGAGCCCAC CAAGACGCAC CGCCTCTCGA ATCCGGCTCT AAAGGCTCTC 
CAGAAGAACG CAGTGCAGTC CTATGTGGAA CGACAGCAGC AGCAGCAGAA GGAGGAACAG 
CAGCTACTAC GTCCGCACTC GCAATCCTAC CAAGCGTGTC ATGTGGAGCG CAAATCACTG 
CCGAACAACT TGAGTCCCAT AATGGTGGGT CTGCCCACTG GGAGTAACTC CGCATCGACT 
CGGGACTGCA GTTCACCCAC TCCACCACCA CCGCCACGAC GTTCGGGGAG TCTGCTGCCC 
AATCTGCTAA GGCGCTCCAG TTCGGCCTCG GACTACGCGG AGTTCAGGGA GCTGCATCAG 
GCACAGGGTC AGGTCAAGGG ACCGAGCATT AGGAACATAA GCAATGCCGA GAAAATCTCC 
TTCAATGACT GCGGAATGCC ACCTCCGCCG CCGCCACCAC GAGGACGTTT GGCCGTGCCG 
ACCAGACGCA CATCCTCGGC AACGGAATAC GCACCCATGC GGGACAAACT GCTGTTGCAG 
CAGGCCGCCG CCTTGGCCCA CCAGCAGCAC CACCCGCAGC AGCATCGCCA TGCCCAACCG 
CCCCATGTGC CGCCCGAGCG TCCGCCCAAG CATCCCAATC TTCGGGTGCC GTCGCCTGAG 
CTGCCACCGC CGCCGCAGAG TGAACTTGAC ATCAGTTATA CCTTCGATGA GCCATTGCCG 
CCGCCACCGC CGCCGGAAGT GCTCCAGCCA CGCCCACCGC CCTCGCCCAA CCGGCGGAAT 
TGCTTCGCCG GAGCATCCAC ACGTCGCACC ACCTACGAAG CACCACCGCC CACCGCAATT 
GTCGCCGCCA AGGTGCCACC GCTGGTGCCC AAGAAGCCAA CGAGCTTGCA GCACAAGCAT 
CTCGCCAACG GAGGAGGCGG CAGTCGCAAG CGCCCGCACC ACGCGACTCC ACAGCCCATC 
CTCGAAAATG TGGCCAGTCC CGTGGCGCCA CCGCCGCCCC TGTTGCCGCG TGCCAGATCC 
ACCGCCCATG ACAATGTGAT TGCCAGCAAT CTGGAGAGCA ACCAGCAGAA ACGATCGAAT 
TCCAAGGCCT CATACTTGCC GCGTCAGAGT CTGGAGAAGT TGAACAACAC TGATCCCGAC 
CATGGCATAT ACAAGCTCAC CCTGACCTCC AACGAGGACT TGGTGGCCCA CACGAAGCCC 
AGCTATGGGG TCACAGGAAA GCTGCCCAAC AATCTGCCGG ATGTCCTGCC GCTGGGCGTT 
AAGCTCCACC AGCAGCCAAA GTTGCAGCCA GGATCGCCGA ACGGCGATGC GAATGTGACC 
CTGCGCTATG GCTCCAACAA CAATCTGACT GGGAATTCCC CGACGGTTGC CCCGCCCCCC 
TACTATGGGG GCGGCCAGCG GTATTCAACT CCTGTGCTGG GTCAAGGTTA CGGCAAAAGT 
TCGAAGCCCG TGACCCCGCA ACAATATACG AGATCTCAGT CGTACGATGT GAAGCACACT 
AGTGCGGTGA CTATGCCGAC AATGTCCCAG TCCCACGTGG ATCTCAAGCA GGCCGCCCAT 
GACCTAGAGA CGACGCTGGA GGAGGTGCTG CCCACTGCCA CGCCCACGCC GACGCCAACA 
CCGACGCCCA CACCGCCACG CCTCTCGCCG GCTTCCTCGC ACTCGGACTG CAGTCTGAGC 
ACCAGTTCCT TGGAGTGCAC AATCAATCCT ATAGCGACAC CGATTCCTAA GCCTGAGGCG 
CACATCTTTC GCGCCGAGGT GATTAGCACC ACCCTGAACA CAAATCCGTT GACAACACCG 
CCCAAGCCCG CGATGAACCG CCAGGAATCC CTGAGGGAGA ACATCGAAAA GATCACCCAA 
CTACAGTCGG TGCTGATGTC GGCGCACCTG TGTGATGCGA GTCTACTAGG TGGTTACACC 
ACTCCACTGA TAACCAGTCC CACTGCCAGT TTCGCTAACG AACCACTAAT GACACCACCA 
CTGCCGCCCA GTCCGCCACC GCCACTAGAA CCGGAGGAGG AGGAGGAGCA GGAGGAGAAC 
GATGTGCACG ACAAGCAGCC AGAGATCGAG GAACTGCAGC TGATGCAGCG CAGCGAATTG 
GTCCTAATGG TGAATCCCAA GCCGAGCACA ACGGATATGG CCTGCCAAAC GGACGAGCTG 
GAGGACAGGG ACACGGACCT CGAAGCGGCA CGCGAGGAGC ACCAGACTAG AACGACTCTG 
CAGCCGCGAC AGCGCCAGCC CATCGAGCTG GACTACGAGC AGATGAGCCG GGAGCTGGTT 
AAGCTCCTAC CGCCTGGTGA CAAGATCGCC GACATCCTCA CACCAAAGAT CTGCAAGCCC 
ACCTCGCAAT ACGTTAGCAA TCTGTACAAT CCGGATGTGC CACTGCGCTT GGCCAAGCGC 
GATGTTGGCA CCTCTACGTT GATGCGAATG AAGTCCATCA CGTCGTCTGC CGAGATCCGA 
GTGGTCAGTG TGGAGCTGCA GCTGGCAGAG CCGAGCGAGG AGCCGACGAA TTTAATCAAG 
CAAAAGATGG ATGAGCTCAT CAAGCATTTG AACCAAAAAA TTGTCTCCCT GAAACGCGAG 
CAGCAGACGA TCAGCGAGGA GTGCTCGGCC AATGACAGAC TGGGCCAGGA TCTATTCGCC 
AAGCTAGCGG AGAAGGTTCG ACCCAGCGAA GCCTCCAAGT TCCGTACCCA TGTCGACGCC 
GTGGGCAACA TAACCAGTTT ACTTCTGTCG CTTTCCGAGC GTTTGGCCCA AACCGAAAGC 
AGCCTGGAAA CGCGCCAGCA GGAAAGGGGC GCGCTGGAAT CAAAGCGGGA TCTGCTGTAC 
GAGCAGATGG AGGAGGCGCA GCGTCTCAAA TCGGACATAG AACGACGTGG AGTCAGCATC 
GCCGGATTAC TGGCCAAGAA CCTCAGCGCG GACATGTGCG CCGACTACGA CTACTTCATC 
AACATGAAGG CCAAGCTGAT CGCCGATGCA CGCGACCTGG CCGTAAGGAT CAAGGGCAGC 
GAGGAGCAGC TTAGCTCCCT CAGCGATGCG CTAGTCCAAA GCGATTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Shrm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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