vg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	vg
Entrez Gene ID	36421
Full Name	vestigial
Synonyms	CG3830,Dmel\CG3830,VG,Vg,vg21
General protein information	Preferred Names vestigial Names CG3830 gene product from transcript CG3830-RA CG3830-PA vestgial vestigal vg-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49E1-49E1\|2-67.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078999.4	NP_523723.1	vestigial

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

here we examine the interactions between Msk and the Wg pathway in regulation of the AMP pool size. We find that a myoblast-specific reduction of Msk results in the absence of Vg expression and a complete loss of the Wg pathway readout beta-catenin/Armadillo (Arm). Moreover, msk RNA interference knockdown abolishes expression of the Wg target Ladybird (Lbe) in leg disc myoblasts.
Title: Adult Muscle Formation Requires

Vestigial protein phosphorylation of serine 215 occurs in the nucleus and requires the presence of Scalloped.
Title: The activity of the Drosophila Vestigial protein is modified by Scalloped-dependent phosphorylation.

High hydrostatic pressure-induced wing mutagenesis in Drosophila melanogaster resulted in 285 differentially expressed genes and two point mutations in the vestigial gene.
Title: Molecular Mechanisms for High Hydrostatic Pressure-Induced Wing Mutagenesis in Drosophila melanogaster.

Data show that genes araucan and caupolican as novel muscle identity genes that confer lateral transverse muscle identity, and the acquisition of this fate requires that Araucan/Caupolican repress other muscle identity genes such as slouch and vestigial.
Title: Drosophila araucan and caupolican integrate intrinsic and signalling inputs for the acquisition by muscle progenitors of the lateral transverse fate.

Studies indicate that negative regulation of the Vestigial gradient by Wingless signaling makes vestigial expression precise and robust.
Title: Spatiotemporally modulated Vestigial gradient by Wingless signaling adaptively regulates cell division for precise wing size control.

The following vg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12943	NM_078999.4 Latest version!	Drosophila melanogaster vestigial (vg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vg ( NM_078999.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523723.1 Drosophila melanogaster vestigial (vg), mRNA.

Price & Availability↑

CloneID	OFa12943
Clone ID Related Accession (Same CDS sequence)	NM_078999.4
Accession Version	NM_078999.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vestigial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_078999.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGTGT CCTGCCCCGA AGTTATGTAC GGTGCCTACT ATCCATATCT GTACGGGCGC 
GCTGGAACAA GTCGATCGTT CTACCAGTAT GAGAGGTTCA ACCAGGATTT GTACTCCTCC 
TCGGGCGTCA ACTTGGCCGC CTCATCGAGT GCCTCGGGCA GCTCCCACTC GCCCTGCAGC 
CCCATCCTGC CGCCATCGGT GAGCGCAAAT GCCGCTGCGG CAGTGGCAGC GGCGGCCCAC 
AATAGTGCAG CGGCGGCAGT GGCGGTCGCA GCGAATCAGG CCAGCTCCTC CGGCGGAATC 
GGAGGCGGTG GACTCGGCGG CCTGGGAGGA CTGGGCGGCG GACCGGCGAG CGGACTGCTT 
GGCAGCAATG TGGTGCCCGG GAGCAGTAGT GTCGGGAGCG TGGGGCTGGG AATGAGTCCG 
GTGCTGAGCG GAGCGGCGGG ACACTCGCTG CACAGTTCGC ACAGGACGCA CGCACACTCC 
CTCGCCCACG CACACACGCA CCCGCACTCG CACACACACA CGCATACGCA TCAAACCAAA 
GAAGAGGACC TCATCGTGCC GCGCAGCGAA GCTGAAGCAC GCCTGGTGGG CTCCCAACAA 
CATCAGCATC ATAATGAATC ATCCTGCTCC TCCGGTCCGG ACTCCCCGCG CCACGCCCAC 
TCGCACAGCC ATCCGCTGCA CGGTGGCGGC GGAGCGACGG GCGGTCCATC TTCCGCGGGT 
GGTACGGGCT CGGGGGGCGG CGACGGAGGC GGCACGGGGG CAATACCCAA GAATCTGCCA 
GCACTGGAGA CGCCGATGGG CAGCGGAGGC GGCGGATTGG CCGGCAGTGG CCAGGGACAG 
GCTCAATATC TATCGGCCTC CTGCGTTGTG TTCACCAACT ACTCGGGCGA CACGGCCAGC 
CAGGTGGACG AGCACTTTTC CCGCGCCCTC AACTACAACA ACAAGGATTC TAAAGAGAGC 
AGCAGCCCGA TGTCGAATCG AAATTTCCCA CCGTCGTTCT GGAACAGCAA TTACGTGCAC 
CCCATACCCG CGCCCACACA CCACCAGGTT AGCGACTTGT ATGGCACCGC GACGGACACC 
GGCTACGCCA CCGATCCGTG GGTGCCGCAT GCGGCCCACT ATGGTTCCTA TGCCCACGCA 
GCGCACGCCC ATGCGGCCCA CGCCCACGCC TACCACCACA ACATGGCCCA GTACGGCAGT 
CTCCTCAGGC TGCCCCAGCA GTACGCCAGC CACGGTTCCA GGCTGCACCA CGACCAGCAG 
ACGGCCCACG CCCTGGAGTA CTCCAGCTAT CCCACAATGG CAGGCCTGGA AGCGCAGGTG 
GCGCAAGTAC AGGAATCGTC GAAGGATCTT TACTGGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523723.1
CDS	1457..2818
Translation	MAVSCPEVMYGAYYPYLYGRAGTSRSFYQYERFNQDLYSSSGVNLAASSSASGSSHSPCSPILPPSVSANAAAAVAAAAHNSAAAAVAVAANQASSSGGIGGGGLGGLGGLGGGPASGLLGSNVVPGSSSVGSVGLGMSPVLSGAAGHSLHSSHRTHAHSLAHAHTHPHSHTHTHTHQTKEEDLIVPRSEAEARLVGSQQHQHHNESSCSSGPDSPRHAHSHSHPLHGGGGATGGPSSAGGTGSGGGDGGGTGAIPKNLPALETPMGSGGGGLAGSGQGQAQYLSASCVVFTNYSGDTASQVDEHFSRALNYNNKDSKESSSPMSNRNFPPSFWNSNYVHPIPAPTHHQVSDLYGTATDTGYATDPWVPHAAHYGSYAHAAHAHAAHAHAYHHNMAQYGSLLRLPQQYASHGSRLHHDQQTAHALEYSSYPTMAGLEAQVAQVQESSKDLYWF

Target ORF information:

RefSeq Version	NM_078999.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vestigial (vg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078999.4

ORF Insert Sequence:

ATGGCAGTGT CCTGCCCCGA AGTTATGTAC GGTGCCTACT ATCCATATCT GTACGGGCGC 
GCTGGAACAA GTCGATCGTT CTACCAGTAT GAGAGGTTCA ACCAGGATTT GTACTCCTCC 
TCGGGCGTCA ACTTGGCCGC CTCATCGAGT GCCTCGGGCA GCTCCCACTC GCCCTGCAGC 
CCCATCCTGC CGCCATCGGT GAGCGCAAAT GCCGCTGCGG CAGTGGCAGC GGCGGCCCAC 
AATAGTGCAG CGGCGGCAGT GGCGGTCGCA GCGAATCAGG CCAGCTCCTC CGGCGGAATC 
GGAGGCGGTG GACTCGGCGG CCTGGGAGGA CTGGGCGGCG GACCGGCGAG CGGACTGCTT 
GGCAGCAATG TGGTGCCCGG GAGCAGTAGT GTCGGGAGCG TGGGGCTGGG AATGAGTCCG 
GTGCTGAGCG GAGCGGCGGG ACACTCGCTG CACAGTTCGC ACAGGACGCA CGCACACTCC 
CTCGCCCACG CACACACGCA CCCGCACTCG CACACACACA CGCATACGCA TCAAACCAAA 
GAAGAGGACC TCATCGTGCC GCGCAGCGAA GCTGAAGCAC GCCTGGTGGG CTCCCAACAA 
CATCAGCATC ATAATGAATC ATCCTGCTCC TCCGGTCCGG ACTCCCCGCG CCACGCCCAC 
TCGCACAGCC ATCCGCTGCA CGGTGGCGGC GGAGCGACGG GCGGTCCATC TTCCGCGGGT 
GGTACGGGCT CGGGGGGCGG CGACGGAGGC GGCACGGGGG CAATACCCAA GAATCTGCCA 
GCACTGGAGA CGCCGATGGG CAGCGGAGGC GGCGGATTGG CCGGCAGTGG CCAGGGACAG 
GCTCAATATC TATCGGCCTC CTGCGTTGTG TTCACCAACT ACTCGGGCGA CACGGCCAGC 
CAGGTGGACG AGCACTTTTC CCGCGCCCTC AACTACAACA ACAAGGATTC TAAAGAGAGC 
AGCAGCCCGA TGTCGAATCG AAATTTCCCA CCGTCGTTCT GGAACAGCAA TTACGTGCAC 
CCCATACCCG CGCCCACACA CCACCAGGTT AGCGACTTGT ATGGCACCGC GACGGACACC 
GGCTACGCCA CCGATCCGTG GGTGCCGCAT GCGGCCCACT ATGGTTCCTA TGCCCACGCA 
GCGCACGCCC ATGCGGCCCA CGCCCACGCC TACCACCACA ACATGGCCCA GTACGGCAGT 
CTCCTCAGGC TGCCCCAGCA GTACGCCAGC CACGGTTCCA GGCTGCACCA CGACCAGCAG 
ACGGCCCACG CCCTGGAGTA CTCCAGCTAT CCCACAATGG CAGGCCTGGA AGCGCAGGTG 
GCGCAAGTAC AGGAATCGTC GAAGGATCTT TACTGGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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vg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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