sca cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sca
Entrez Gene ID	36411
Full Name	scabrous
Synonyms	AAA28880,AAF58455,CG17579,Dmel\CG17579,FBpp0086968,FBpp0086969,Sca,Sca1b,anon-EST:Liang-38,clone 38,mAb sca1
General protein information	Preferred Names scabrous Names CG17579 gene product from transcript CG17579-RB CG17579-PA CG17579-PB Fibrinogen-related protein sca-PA sca-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49C3-49D3\|2-66.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_165951.2	NP_725230.2	scabrous, isoform B
NM_057362.3	NP_476710.2	scabrous, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mfap4	NP_083844.1
Canis lupus familiaris (dog)	MFAP4	XP_546650.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007031	XP_565171.3
Xenopus tropicalis (tropical clawed frog)	LOC100488644	XP_002943442.2
Xenopus tropicalis (tropical clawed frog)	LOC100488329	XP_002943440.2
Bos taurus (cattle)	MFAP4	NP_001073686.1
Rattus norvegicus (Norway rat)	Mfap4	NP_001029296.1
Drosophila melanogaster (fruit fly)	sca	NP_725230.2
Homo sapiens (human)	MFAP4	NP_001185624.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Scabrous is an activator that can overcome the sensitivity of template-based lateral inhibition to spatial variations in biochemical parameters and cell sizes in eye development.
Title: Periodic patterning of the Drosophila eye is stabilized by the diffusible activator Scabrous.

These and other results obtained with a reporter line of Notch activity indicate that sca overexpression antagonizes Notch signaling in the Drosophila eye, and are inconsistent with Sca being an ommatidial rotation-specific factor.
Title: Scabrous overexpression in the eye affects R3/R4 cell fate specification and inhibits notch signaling.

Notch, Delta, and Scabrous might function in complex combinatorial or mutually exclusive interactions during Drosophila development.
Title: Delta activity independent of its activity as a ligand of Notch.

Data suggest that endosomal Scabrous and Gp150 promote Notch activation in response to Delta, by regulating acquisition of insensitivity to Delta in a subset of cells.
Title: Scabrous and Gp150 are endosomal proteins that regulate Notch activity.

Scabrous controls the stop of ommatidial rotation by suppressing nemo activity in the second 45 degrees rotation. The morphogenetic furrow regulates precise ommatidial rotation by transporting Scabrous through actin-based cellular extensions.
Title: Scabrous controls ommatidial rotation in the Drosophila compound eye.

The following sca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09817	NM_057362.3 Latest version!	Drosophila melanogaster scabrous (sca), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09817	NM_165951.2 Latest version!	Drosophila melanogaster scabrous (sca), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sca ( NM_165951.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725230.2 Drosophila melanogaster scabrous (sca), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09817
Clone ID Related Accession (Same CDS sequence)	NM_165951.2 , NM_057362.3
Accession Version	NM_165951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scabrous, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165951.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAGATT GGCAAACATT CCCGGACCTC CAAAAAAAGA AAGTTTCGCG TGACCATTTA 
AATTGCCCTG CAACAATGGC AGGTTCAAAC GTTTTGTGGC CAATACTCCT GGCCGTGGTG 
CTGCTCCAAA TATCCGTGGC ATTCGTGAGT GGAGCGGCCA GTGGTGGGGT AGTCCTTAGC 
GACGTGAACA ACATGTTGCG CGATGCCAAG GTGGTGACCT CGGAGAAACC CGTTGTGCAC 
TCAAAACAGG AAACGGAAGC GCCGGAATCC AGCGTGGAGC TGCTCCGCTT CGTCGATGAT 
GACGAGGATA GCGAGGACAT CAGCTCCATT GAACGGCAGG ATGGCAGGAC AATGGAGAGC 
AAAAAAATGG CCGATCAGGT GCGCCTGTTG ACCAAGCAGC TCAACGCCCT GATGCTGCGG 
CGCCGCGAGG ATTACGAGAT GCTGGAGCAC AATCTGCGCA AATCCCTGCG GCTCACCACG 
AATGCGAACA GCGTGGACGC CGACATGCGC AGCGAACTGA ACCAACTCAG GGAGGAGCTG 
GCCGCGCTGC GCTCCTCGCA GAGTGGCAAC AAGGAGCGCT TGACCGTCGA GTGGCTGCAG 
CAGACGATCT CCGAGATCCG CAAACAGCTG GTGGATCTGC AAAGGACGGC CAGCAACGTG 
GCCCAGGATG TCCAGCAGCG CAGCTCCACC TTTGAGGATC TGGCCACCAT TCGCAGTGAC 
TATCAGCAGC TTAAGCTAGA TCTGGCCGCT CAGCGCGAGC GCCAGCAGCA GACGGAGGTC 
TACGTCCAGG AACTGCGCGA GGAGATGCTC CAGCAGGAGC AGGACTTCCA GCATGCCCTC 
GTCAAGCTGC AGCAGAGGAC TCGCAAGGAC GGCTCATCCG CCAGTGTGGA GGAGGAGAGC 
GGTAGCCAGG AAGCCAACCA GGAGCAAACC GGACTTGAAA CCACTGCTGA TCACAAGCGA 
CGCCATTGCC GTTTTCAGAG CGAACAGATC CACCAGCTGC AACTGGCCCA GAGGAACCTG 
CGCCGACAGG TGAACGGATT GCGCTTCCAC CACATCGACG AGCGGGTTCG CAGCATCGAG 
GTGGAGCAGC ACAGGATTGC CAATGCCAAC TTTAATTTGA GCAGCCAGAT CGCTTCGCTG 
GACAAGCTGC ATACCTCGAT GCTGGAGCTG CTGGAAGATG TGGAGGGCCT CCAGACCAAG 
ATGGACAAGA GCATACCGGA GCTGCGGCAC GAGATCTCCA AGCTGGAGTT CGCCAATGCT 
CAGATCACCT CGGAGCAGAG TCTGATCAGG GAGGAGGGCA CTAATGCGGC ACGATCCCTG 
CAAGCCATGG CTGTAAGCGT CAGTGTCCTG CAGGAGGAGC GCGAAGGTAT GCGGAAGCTG 
TCCGCCAATG TGGATCAGCT GAGAACCAAT GTGGATCGAT TGCAGTCGCT GGTCAATGAT 
GAAATGAAGA ATAAGCTCAC CCACCTGAAC AAGCCGCACA AGCGACCACA TCATCAGAAT 
GTCCAGGCGC AGATGCCGCA GGATGATTCG CCCATTGACT CCGTCCTGGC CGAAACTCTG 
GTCAGCGAGC TTGAGAACGT GGAGACCCAG TACGAGGCCA TTATCAACAA ACTGCCGCAC 
GACTGCAGCG AGGTGCACAC TCAAACAGAC GGACTGCATC TGATTGCGCC CGCCGGCCAA 
CGGCATCCGC TGATGACGCA CTGCACCGCC GATGGATGGA CGACGGTGCA AAGGCGGTTC 
GATGGCAGTG CAGACTTCAA CCGCTCGTGG GCGGATTATG CCCAAGGATT TGGGGCGCCA 
GGCGGTGAAT TCTGGATTGG CAACGAGCAG CTGCATCACC TGACCCTGGA CAACTGCAGT 
CGGCTGCAGG TGCAAATGCA GGACATCTAC GACAACGTTT GGGTGGCCGA GTACAAGCGA 
TTCTACATAT CCTCGCGAGC CGATGGCTAT CGGCTGCACA TTGCCGAGTA CTCCGGCAAC 
GCTTCGGATG CACTGAACTA CCAACAGGGT ATGCAGTTCT CGGCCATCGA TGACGATCGG 
GACATCTCGC AGACGCACTG TGCTGCTAAC TATGAGGGTG GCTGGTGGTT CTCTCATTGC 
CAGCACGCCA ATCTCAATGG GCGATACAAT CTGGGCCTGA CTTGGTTCGA TGCCGCTCGC 
AATGAATGGA TAGCGGTCAA GTCAAGCCGA ATGCTGGTCA AGCGCCTGCC CGCCGTCGAG 
TGCCAGGCGA ATGCCAGTGC CAGTGGCGCT TTTGTTTCCG TTTCCGGTTC GGCTGCTGAT 
GCTGCGCCGT CGAGCGGTGC AACAACAACA ACAACAACAG CAACAGCAGC GCCGGCGACG 
GTAACGACGC CGAAAACCAA CAACAGTGTG GTCCAGTTCG TGGCCGCCGG GCAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725230.2
CDS	323..2722
Translation	MRDWQTFPDLQKKKVSRDHLNCPATMAGSNVLWPILLAVVLLQISVAFVSGAASGGVVLSDVNNMLRDAKVVTSEKPVVHSKQETEAPESSVELLRFVDDDEDSEDISSIERQDGRTMESKKMADQVRLLTKQLNALMLRRREDYEMLEHNLRKSLRLTTNANSVDADMRSELNQLREELAALRSSQSGNKERLTVEWLQQTISEIRKQLVDLQRTASNVAQDVQQRSSTFEDLATIRSDYQQLKLDLAAQRERQQQTEVYVQELREEMLQQEQDFQHALVKLQQRTRKDGSSASVEEESGSQEANQEQTGLETTADHKRRHCRFQSEQIHQLQLAQRNLRRQVNGLRFHHIDERVRSIEVEQHRIANANFNLSSQIASLDKLHTSMLELLEDVEGLQTKMDKSIPELRHEISKLEFANAQITSEQSLIREEGTNAARSLQAMAVSVSVLQEEREGMRKLSANVDQLRTNVDRLQSLVNDEMKNKLTHLNKPHKRPHHQNVQAQMPQDDSPIDSVLAETLVSELENVETQYEAIINKLPHDCSEVHTQTDGLHLIAPAGQRHPLMTHCTADGWTTVQRRFDGSADFNRSWADYAQGFGAPGGEFWIGNEQLHHLTLDNCSRLQVQMQDIYDNVWVAEYKRFYISSRADGYRLHIAEYSGNASDALNYQQGMQFSAIDDDRDISQTHCAANYEGGWWFSHCQHANLNGRYNLGLTWFDAARNEWIAVKSSRMLVKRLPAVECQANASASGAFVSVSGSAADAAPSSGATTTTTTATAAPATVTTPKTNNSVVQFVAAGQA

Target ORF information:

RefSeq Version	NM_165951.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scabrous (sca), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165951.2

ORF Insert Sequence:

ATGAGAGATT GGCAAACATT CCCGGACCTC CAAAAAAAGA AAGTTTCGCG TGACCATTTA 
AATTGCCCTG CAACAATGGC AGGTTCAAAC GTTTTGTGGC CAATACTCCT GGCCGTGGTG 
CTGCTCCAAA TATCCGTGGC ATTCGTGAGT GGAGCGGCCA GTGGTGGGGT AGTCCTTAGC 
GACGTGAACA ACATGTTGCG CGATGCCAAG GTGGTGACCT CGGAGAAACC CGTTGTGCAC 
TCAAAACAGG AAACGGAAGC GCCGGAATCC AGCGTGGAGC TGCTCCGCTT CGTCGATGAT 
GACGAGGATA GCGAGGACAT CAGCTCCATT GAACGGCAGG ATGGCAGGAC AATGGAGAGC 
AAAAAAATGG CCGATCAGGT GCGCCTGTTG ACCAAGCAGC TCAACGCCCT GATGCTGCGG 
CGCCGCGAGG ATTACGAGAT GCTGGAGCAC AATCTGCGCA AATCCCTGCG GCTCACCACG 
AATGCGAACA GCGTGGACGC CGACATGCGC AGCGAACTGA ACCAACTCAG GGAGGAGCTG 
GCCGCGCTGC GCTCCTCGCA GAGTGGCAAC AAGGAGCGCT TGACCGTCGA GTGGCTGCAG 
CAGACGATCT CCGAGATCCG CAAACAGCTG GTGGATCTGC AAAGGACGGC CAGCAACGTG 
GCCCAGGATG TCCAGCAGCG CAGCTCCACC TTTGAGGATC TGGCCACCAT TCGCAGTGAC 
TATCAGCAGC TTAAGCTAGA TCTGGCCGCT CAGCGCGAGC GCCAGCAGCA GACGGAGGTC 
TACGTCCAGG AACTGCGCGA GGAGATGCTC CAGCAGGAGC AGGACTTCCA GCATGCCCTC 
GTCAAGCTGC AGCAGAGGAC TCGCAAGGAC GGCTCATCCG CCAGTGTGGA GGAGGAGAGC 
GGTAGCCAGG AAGCCAACCA GGAGCAAACC GGACTTGAAA CCACTGCTGA TCACAAGCGA 
CGCCATTGCC GTTTTCAGAG CGAACAGATC CACCAGCTGC AACTGGCCCA GAGGAACCTG 
CGCCGACAGG TGAACGGATT GCGCTTCCAC CACATCGACG AGCGGGTTCG CAGCATCGAG 
GTGGAGCAGC ACAGGATTGC CAATGCCAAC TTTAATTTGA GCAGCCAGAT CGCTTCGCTG 
GACAAGCTGC ATACCTCGAT GCTGGAGCTG CTGGAAGATG TGGAGGGCCT CCAGACCAAG 
ATGGACAAGA GCATACCGGA GCTGCGGCAC GAGATCTCCA AGCTGGAGTT CGCCAATGCT 
CAGATCACCT CGGAGCAGAG TCTGATCAGG GAGGAGGGCA CTAATGCGGC ACGATCCCTG 
CAAGCCATGG CTGTAAGCGT CAGTGTCCTG CAGGAGGAGC GCGAAGGTAT GCGGAAGCTG 
TCCGCCAATG TGGATCAGCT GAGAACCAAT GTGGATCGAT TGCAGTCGCT GGTCAATGAT 
GAAATGAAGA ATAAGCTCAC CCACCTGAAC AAGCCGCACA AGCGACCACA TCATCAGAAT 
GTCCAGGCGC AGATGCCGCA GGATGATTCG CCCATTGACT CCGTCCTGGC CGAAACTCTG 
GTCAGCGAGC TTGAGAACGT GGAGACCCAG TACGAGGCCA TTATCAACAA ACTGCCGCAC 
GACTGCAGCG AGGTGCACAC TCAAACAGAC GGACTGCATC TGATTGCGCC CGCCGGCCAA 
CGGCATCCGC TGATGACGCA CTGCACCGCC GATGGATGGA CGACGGTGCA AAGGCGGTTC 
GATGGCAGTG CAGACTTCAA CCGCTCGTGG GCGGATTATG CCCAAGGATT TGGGGCGCCA 
GGCGGTGAAT TCTGGATTGG CAACGAGCAG CTGCATCACC TGACCCTGGA CAACTGCAGT 
CGGCTGCAGG TGCAAATGCA GGACATCTAC GACAACGTTT GGGTGGCCGA GTACAAGCGA 
TTCTACATAT CCTCGCGAGC CGATGGCTAT CGGCTGCACA TTGCCGAGTA CTCCGGCAAC 
GCTTCGGATG CACTGAACTA CCAACAGGGT ATGCAGTTCT CGGCCATCGA TGACGATCGG 
GACATCTCGC AGACGCACTG TGCTGCTAAC TATGAGGGTG GCTGGTGGTT CTCTCATTGC 
CAGCACGCCA ATCTCAATGG GCGATACAAT CTGGGCCTGA CTTGGTTCGA TGCCGCTCGC 
AATGAATGGA TAGCGGTCAA GTCAAGCCGA ATGCTGGTCA AGCGCCTGCC CGCCGTCGAG 
TGCCAGGCGA ATGCCAGTGC CAGTGGCGCT TTTGTTTCCG TTTCCGGTTC GGCTGCTGAT 
GCTGCGCCGT CGAGCGGTGC AACAACAACA ACAACAACAG CAACAGCAGC GCCGGCGACG 
GTAACGACGC CGAAAACCAA CAACAGTGTG GTCCAGTTCG TGGCCGCCGG GCAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sca ( NM_057362.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476710.2 Drosophila melanogaster scabrous (sca), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09817
Clone ID Related Accession (Same CDS sequence)	NM_165951.2 , NM_057362.3
Accession Version	NM_057362.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scabrous, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057362.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAGATT GGCAAACATT CCCGGACCTC CAAAAAAAGA AAGTTTCGCG TGACCATTTA 
AATTGCCCTG CAACAATGGC AGGTTCAAAC GTTTTGTGGC CAATACTCCT GGCCGTGGTG 
CTGCTCCAAA TATCCGTGGC ATTCGTGAGT GGAGCGGCCA GTGGTGGGGT AGTCCTTAGC 
GACGTGAACA ACATGTTGCG CGATGCCAAG GTGGTGACCT CGGAGAAACC CGTTGTGCAC 
TCAAAACAGG AAACGGAAGC GCCGGAATCC AGCGTGGAGC TGCTCCGCTT CGTCGATGAT 
GACGAGGATA GCGAGGACAT CAGCTCCATT GAACGGCAGG ATGGCAGGAC AATGGAGAGC 
AAAAAAATGG CCGATCAGGT GCGCCTGTTG ACCAAGCAGC TCAACGCCCT GATGCTGCGG 
CGCCGCGAGG ATTACGAGAT GCTGGAGCAC AATCTGCGCA AATCCCTGCG GCTCACCACG 
AATGCGAACA GCGTGGACGC CGACATGCGC AGCGAACTGA ACCAACTCAG GGAGGAGCTG 
GCCGCGCTGC GCTCCTCGCA GAGTGGCAAC AAGGAGCGCT TGACCGTCGA GTGGCTGCAG 
CAGACGATCT CCGAGATCCG CAAACAGCTG GTGGATCTGC AAAGGACGGC CAGCAACGTG 
GCCCAGGATG TCCAGCAGCG CAGCTCCACC TTTGAGGATC TGGCCACCAT TCGCAGTGAC 
TATCAGCAGC TTAAGCTAGA TCTGGCCGCT CAGCGCGAGC GCCAGCAGCA GACGGAGGTC 
TACGTCCAGG AACTGCGCGA GGAGATGCTC CAGCAGGAGC AGGACTTCCA GCATGCCCTC 
GTCAAGCTGC AGCAGAGGAC TCGCAAGGAC GGCTCATCCG CCAGTGTGGA GGAGGAGAGC 
GGTAGCCAGG AAGCCAACCA GGAGCAAACC GGACTTGAAA CCACTGCTGA TCACAAGCGA 
CGCCATTGCC GTTTTCAGAG CGAACAGATC CACCAGCTGC AACTGGCCCA GAGGAACCTG 
CGCCGACAGG TGAACGGATT GCGCTTCCAC CACATCGACG AGCGGGTTCG CAGCATCGAG 
GTGGAGCAGC ACAGGATTGC CAATGCCAAC TTTAATTTGA GCAGCCAGAT CGCTTCGCTG 
GACAAGCTGC ATACCTCGAT GCTGGAGCTG CTGGAAGATG TGGAGGGCCT CCAGACCAAG 
ATGGACAAGA GCATACCGGA GCTGCGGCAC GAGATCTCCA AGCTGGAGTT CGCCAATGCT 
CAGATCACCT CGGAGCAGAG TCTGATCAGG GAGGAGGGCA CTAATGCGGC ACGATCCCTG 
CAAGCCATGG CTGTAAGCGT CAGTGTCCTG CAGGAGGAGC GCGAAGGTAT GCGGAAGCTG 
TCCGCCAATG TGGATCAGCT GAGAACCAAT GTGGATCGAT TGCAGTCGCT GGTCAATGAT 
GAAATGAAGA ATAAGCTCAC CCACCTGAAC AAGCCGCACA AGCGACCACA TCATCAGAAT 
GTCCAGGCGC AGATGCCGCA GGATGATTCG CCCATTGACT CCGTCCTGGC CGAAACTCTG 
GTCAGCGAGC TTGAGAACGT GGAGACCCAG TACGAGGCCA TTATCAACAA ACTGCCGCAC 
GACTGCAGCG AGGTGCACAC TCAAACAGAC GGACTGCATC TGATTGCGCC CGCCGGCCAA 
CGGCATCCGC TGATGACGCA CTGCACCGCC GATGGATGGA CGACGGTGCA AAGGCGGTTC 
GATGGCAGTG CAGACTTCAA CCGCTCGTGG GCGGATTATG CCCAAGGATT TGGGGCGCCA 
GGCGGTGAAT TCTGGATTGG CAACGAGCAG CTGCATCACC TGACCCTGGA CAACTGCAGT 
CGGCTGCAGG TGCAAATGCA GGACATCTAC GACAACGTTT GGGTGGCCGA GTACAAGCGA 
TTCTACATAT CCTCGCGAGC CGATGGCTAT CGGCTGCACA TTGCCGAGTA CTCCGGCAAC 
GCTTCGGATG CACTGAACTA CCAACAGGGT ATGCAGTTCT CGGCCATCGA TGACGATCGG 
GACATCTCGC AGACGCACTG TGCTGCTAAC TATGAGGGTG GCTGGTGGTT CTCTCATTGC 
CAGCACGCCA ATCTCAATGG GCGATACAAT CTGGGCCTGA CTTGGTTCGA TGCCGCTCGC 
AATGAATGGA TAGCGGTCAA GTCAAGCCGA ATGCTGGTCA AGCGCCTGCC CGCCGTCGAG 
TGCCAGGCGA ATGCCAGTGC CAGTGGCGCT TTTGTTTCCG TTTCCGGTTC GGCTGCTGAT 
GCTGCGCCGT CGAGCGGTGC AACAACAACA ACAACAACAG CAACAGCAGC GCCGGCGACG 
GTAACGACGC CGAAAACCAA CAACAGTGTG GTCCAGTTCG TGGCCGCCGG GCAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476710.2
CDS	323..2722
Translation	MRDWQTFPDLQKKKVSRDHLNCPATMAGSNVLWPILLAVVLLQISVAFVSGAASGGVVLSDVNNMLRDAKVVTSEKPVVHSKQETEAPESSVELLRFVDDDEDSEDISSIERQDGRTMESKKMADQVRLLTKQLNALMLRRREDYEMLEHNLRKSLRLTTNANSVDADMRSELNQLREELAALRSSQSGNKERLTVEWLQQTISEIRKQLVDLQRTASNVAQDVQQRSSTFEDLATIRSDYQQLKLDLAAQRERQQQTEVYVQELREEMLQQEQDFQHALVKLQQRTRKDGSSASVEEESGSQEANQEQTGLETTADHKRRHCRFQSEQIHQLQLAQRNLRRQVNGLRFHHIDERVRSIEVEQHRIANANFNLSSQIASLDKLHTSMLELLEDVEGLQTKMDKSIPELRHEISKLEFANAQITSEQSLIREEGTNAARSLQAMAVSVSVLQEEREGMRKLSANVDQLRTNVDRLQSLVNDEMKNKLTHLNKPHKRPHHQNVQAQMPQDDSPIDSVLAETLVSELENVETQYEAIINKLPHDCSEVHTQTDGLHLIAPAGQRHPLMTHCTADGWTTVQRRFDGSADFNRSWADYAQGFGAPGGEFWIGNEQLHHLTLDNCSRLQVQMQDIYDNVWVAEYKRFYISSRADGYRLHIAEYSGNASDALNYQQGMQFSAIDDDRDISQTHCAANYEGGWWFSHCQHANLNGRYNLGLTWFDAARNEWIAVKSSRMLVKRLPAVECQANASASGAFVSVSGSAADAAPSSGATTTTTTATAAPATVTTPKTNNSVVQFVAAGQA

Target ORF information:

RefSeq Version	NM_057362.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scabrous (sca), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057362.3

ORF Insert Sequence:

ATGAGAGATT GGCAAACATT CCCGGACCTC CAAAAAAAGA AAGTTTCGCG TGACCATTTA 
AATTGCCCTG CAACAATGGC AGGTTCAAAC GTTTTGTGGC CAATACTCCT GGCCGTGGTG 
CTGCTCCAAA TATCCGTGGC ATTCGTGAGT GGAGCGGCCA GTGGTGGGGT AGTCCTTAGC 
GACGTGAACA ACATGTTGCG CGATGCCAAG GTGGTGACCT CGGAGAAACC CGTTGTGCAC 
TCAAAACAGG AAACGGAAGC GCCGGAATCC AGCGTGGAGC TGCTCCGCTT CGTCGATGAT 
GACGAGGATA GCGAGGACAT CAGCTCCATT GAACGGCAGG ATGGCAGGAC AATGGAGAGC 
AAAAAAATGG CCGATCAGGT GCGCCTGTTG ACCAAGCAGC TCAACGCCCT GATGCTGCGG 
CGCCGCGAGG ATTACGAGAT GCTGGAGCAC AATCTGCGCA AATCCCTGCG GCTCACCACG 
AATGCGAACA GCGTGGACGC CGACATGCGC AGCGAACTGA ACCAACTCAG GGAGGAGCTG 
GCCGCGCTGC GCTCCTCGCA GAGTGGCAAC AAGGAGCGCT TGACCGTCGA GTGGCTGCAG 
CAGACGATCT CCGAGATCCG CAAACAGCTG GTGGATCTGC AAAGGACGGC CAGCAACGTG 
GCCCAGGATG TCCAGCAGCG CAGCTCCACC TTTGAGGATC TGGCCACCAT TCGCAGTGAC 
TATCAGCAGC TTAAGCTAGA TCTGGCCGCT CAGCGCGAGC GCCAGCAGCA GACGGAGGTC 
TACGTCCAGG AACTGCGCGA GGAGATGCTC CAGCAGGAGC AGGACTTCCA GCATGCCCTC 
GTCAAGCTGC AGCAGAGGAC TCGCAAGGAC GGCTCATCCG CCAGTGTGGA GGAGGAGAGC 
GGTAGCCAGG AAGCCAACCA GGAGCAAACC GGACTTGAAA CCACTGCTGA TCACAAGCGA 
CGCCATTGCC GTTTTCAGAG CGAACAGATC CACCAGCTGC AACTGGCCCA GAGGAACCTG 
CGCCGACAGG TGAACGGATT GCGCTTCCAC CACATCGACG AGCGGGTTCG CAGCATCGAG 
GTGGAGCAGC ACAGGATTGC CAATGCCAAC TTTAATTTGA GCAGCCAGAT CGCTTCGCTG 
GACAAGCTGC ATACCTCGAT GCTGGAGCTG CTGGAAGATG TGGAGGGCCT CCAGACCAAG 
ATGGACAAGA GCATACCGGA GCTGCGGCAC GAGATCTCCA AGCTGGAGTT CGCCAATGCT 
CAGATCACCT CGGAGCAGAG TCTGATCAGG GAGGAGGGCA CTAATGCGGC ACGATCCCTG 
CAAGCCATGG CTGTAAGCGT CAGTGTCCTG CAGGAGGAGC GCGAAGGTAT GCGGAAGCTG 
TCCGCCAATG TGGATCAGCT GAGAACCAAT GTGGATCGAT TGCAGTCGCT GGTCAATGAT 
GAAATGAAGA ATAAGCTCAC CCACCTGAAC AAGCCGCACA AGCGACCACA TCATCAGAAT 
GTCCAGGCGC AGATGCCGCA GGATGATTCG CCCATTGACT CCGTCCTGGC CGAAACTCTG 
GTCAGCGAGC TTGAGAACGT GGAGACCCAG TACGAGGCCA TTATCAACAA ACTGCCGCAC 
GACTGCAGCG AGGTGCACAC TCAAACAGAC GGACTGCATC TGATTGCGCC CGCCGGCCAA 
CGGCATCCGC TGATGACGCA CTGCACCGCC GATGGATGGA CGACGGTGCA AAGGCGGTTC 
GATGGCAGTG CAGACTTCAA CCGCTCGTGG GCGGATTATG CCCAAGGATT TGGGGCGCCA 
GGCGGTGAAT TCTGGATTGG CAACGAGCAG CTGCATCACC TGACCCTGGA CAACTGCAGT 
CGGCTGCAGG TGCAAATGCA GGACATCTAC GACAACGTTT GGGTGGCCGA GTACAAGCGA 
TTCTACATAT CCTCGCGAGC CGATGGCTAT CGGCTGCACA TTGCCGAGTA CTCCGGCAAC 
GCTTCGGATG CACTGAACTA CCAACAGGGT ATGCAGTTCT CGGCCATCGA TGACGATCGG 
GACATCTCGC AGACGCACTG TGCTGCTAAC TATGAGGGTG GCTGGTGGTT CTCTCATTGC 
CAGCACGCCA ATCTCAATGG GCGATACAAT CTGGGCCTGA CTTGGTTCGA TGCCGCTCGC 
AATGAATGGA TAGCGGTCAA GTCAAGCCGA ATGCTGGTCA AGCGCCTGCC CGCCGTCGAG 
TGCCAGGCGA ATGCCAGTGC CAGTGGCGCT TTTGTTTCCG TTTCCGGTTC GGCTGCTGAT 
GCTGCGCCGT CGAGCGGTGC AACAACAACA ACAACAACAG CAACAGCAGC GCCGGCGACG 
GTAACGACGC CGAAAACCAA CAACAGTGTG GTCCAGTTCG TGGCCGCCGG GCAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sca cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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