cuff cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cuff
Entrez Gene ID	36269
Full Name	cutoff
Synonyms	CG13190,Dmel\CG13190
General protein information	Preferred Names cutoff Names CG13190 gene product from transcript CG13190-RA CG13190-PA cuff-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48C5-48C5\|2-61 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_136865.3	NP_610709.1	cutoff

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results confirm that Cutoff is involved in the transcriptional regulation of piRNA clusters and in the repression of transposable elements in germ cells. Surprisingly, however, Cutoff is found enriched at and affects the expression of other noncoding RNAs, including spliceosomal RNAs and small nucleolar RNAs. Finally, mutations in Cutoff result in the deregulation of hundreds of protein-coding genes in germ cells.
Title: Integrative analysis unveils new functions for the

Cutoff suppresses RNA polymerase II termination to ensure expression of piRNA precursors.
Title: Cutoff Suppresses RNA Polymerase II Termination to Ensure Expression of piRNA Precursors.

Analysis of piRNA clusters in the Drosophila ovary shows that hallmarks of RNA Polymerase II (Pol II) transcription are fundamentally different at uni-strand versus dual-strand clusters. Genome-wide, piRNA production from dual-strand clusters requires the RDC complex consisting of Rhi, Deadlock (Del), and Cuff.
Title: The rhino-deadlock-cutoff complex licenses noncanonical transcription of dual-strand piRNA clusters in Drosophila.

results unveil a link between Cuff activity, heterochromatin assembly and piRNA cluster expression, which is critical for stem-cell and germ-cell development in Drosophila
Title: The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline.

The following cuff gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cuff cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09651	NM_136865.3 Latest version!	Drosophila melanogaster cutoff (cuff), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cuff ( NM_136865.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610709.1 Drosophila melanogaster cutoff (cuff), mRNA.

Price & Availability↑

CloneID	OFa09651
Clone ID Related Accession (Same CDS sequence)	NM_136865.3
Accession Version	NM_136865.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cutoff
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_136865.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTCTA ATTACACAAT ATTAAACATC CGGGCTCACT CCTGGGCCGA GAAGGACAAT 
TTGGTATTTC CAACTATTAC CAAGCCCGTT TCGAATGGCT GGTTCGCCTG GTCGCCAATC 
GGGCAATTCG AACCAAATTC TCAAAAAGTG CCCTACGTCG TGGTGCCGCC AAGCATCAAA 
TTTCCCATTT CTCTTCGGTA CAAAGATTCA CCGCCGAAGC TGGAAAAGAA GCCCAACATT 
TTTCTGGACA ACATGCTCCA GTATATAGAA TCTTCGTCGT ACATGTACGT TATGGTAAGA 
AACAACCAGC AGGCACAGCT AAATGCCAAT ATAGTGTGCA CCAGCGAAGT CCTGGAGCTG 
ATGATGTGTG CTCCCTACGA AAAGAAGACT GGATGGTCAT TGGGTGTCAC CCGGTATCGC 
AACACAATGT ACATTTGCCG CATTGATGTT GAACAACCCG ACCCCATTGA TCAGGACAAT 
CTGAAGAGGG CCATGCAAGA ATTTTGGCTT AGGAATCTTC GAACCCATTG TGTTTTTGAA 
AACGGCATTA AGATGCACCA GCACAATCAA TCTTCTGAAG AACATTTACG CTTTCATGGT 
GTATTTTCCT TTGATTTGAA TGGTAATCGA GTTCTATTTG ATTCTCCAGT ACTAGCCGAG 
ATGCCAAGCA CAACATTGAA TGGGTCTGCT CTAAGCTGGG TAGACCTGCA GATACGCCCT 
ATGTTCATGA GTCGACTGGA TTGGCCAGAG CACAATCGCA CAGAGGCCCT TAAGTGGTGG 
GTCAAGTGTT TTCTGTTGGG AATCGAAAGC CTCTATATAG CCCGCCGCGA TGAAAACGCC 
CATGTGCACA ACATTGAAAA GACTTTGGTG CGGGATTTAT GGAAGTCTTG CGAAAAAGAT 
TGGTCGCCCA CAGTGTGCGC CAACTTCATG ATTTACCTAC TCAATTGTAT TTCCCAAGTT 
ATGGCGCCGA TCGACTGCCC CAGCACCGTT TACCTTTTTC AGTTCGACGC GAGCCAAGGA 
ACAGTATCAT ACAAGGGCTT GCGAGGGCGC AATCAGTACA CGTTCGTTTC GGACTGGTTC 
CGGATGATGC TGGACGATCA CACGAACGAT ATGTGTAAGA CACCGAATTT GCAAACCATG 
TCTTCTATAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610709.1
CDS	45..1199
Translation	MNSNYTILNIRAHSWAEKDNLVFPTITKPVSNGWFAWSPIGQFEPNSQKVPYVVVPPSIKFPISLRYKDSPPKLEKKPNIFLDNMLQYIESSSYMYVMVRNNQQAQLNANIVCTSEVLELMMCAPYEKKTGWSLGVTRYRNTMYICRIDVEQPDPIDQDNLKRAMQEFWLRNLRTHCVFENGIKMHQHNQSSEEHLRFHGVFSFDLNGNRVLFDSPVLAEMPSTTLNGSALSWVDLQIRPMFMSRLDWPEHNRTEALKWWVKCFLLGIESLYIARRDENAHVHNIEKTLVRDLWKSCEKDWSPTVCANFMIYLLNCISQVMAPIDCPSTVYLFQFDASQGTVSYKGLRGRNQYTFVSDWFRMMLDDHTNDMCKTPNLQTMSSIV

Target ORF information:

RefSeq Version	NM_136865.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cutoff (cuff), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136865.3

ORF Insert Sequence:

ATGAATTCTA ATTACACAAT ATTAAACATC CGGGCTCACT CCTGGGCCGA GAAGGACAAT 
TTGGTATTTC CAACTATTAC CAAGCCCGTT TCGAATGGCT GGTTCGCCTG GTCGCCAATC 
GGGCAATTCG AACCAAATTC TCAAAAAGTG CCCTACGTCG TGGTGCCGCC AAGCATCAAA 
TTTCCCATTT CTCTTCGGTA CAAAGATTCA CCGCCGAAGC TGGAAAAGAA GCCCAACATT 
TTTCTGGACA ACATGCTCCA GTATATAGAA TCTTCGTCGT ACATGTACGT TATGGTAAGA 
AACAACCAGC AGGCACAGCT AAATGCCAAT ATAGTGTGCA CCAGCGAAGT CCTGGAGCTG 
ATGATGTGTG CTCCCTACGA AAAGAAGACT GGATGGTCAT TGGGTGTCAC CCGGTATCGC 
AACACAATGT ACATTTGCCG CATTGATGTT GAACAACCCG ACCCCATTGA TCAGGACAAT 
CTGAAGAGGG CCATGCAAGA ATTTTGGCTT AGGAATCTTC GAACCCATTG TGTTTTTGAA 
AACGGCATTA AGATGCACCA GCACAATCAA TCTTCTGAAG AACATTTACG CTTTCATGGT 
GTATTTTCCT TTGATTTGAA TGGTAATCGA GTTCTATTTG ATTCTCCAGT ACTAGCCGAG 
ATGCCAAGCA CAACATTGAA TGGGTCTGCT CTAAGCTGGG TAGACCTGCA GATACGCCCT 
ATGTTCATGA GTCGACTGGA TTGGCCAGAG CACAATCGCA CAGAGGCCCT TAAGTGGTGG 
GTCAAGTGTT TTCTGTTGGG AATCGAAAGC CTCTATATAG CCCGCCGCGA TGAAAACGCC 
CATGTGCACA ACATTGAAAA GACTTTGGTG CGGGATTTAT GGAAGTCTTG CGAAAAAGAT 
TGGTCGCCCA CAGTGTGCGC CAACTTCATG ATTTACCTAC TCAATTGTAT TTCCCAAGTT 
ATGGCGCCGA TCGACTGCCC CAGCACCGTT TACCTTTTTC AGTTCGACGC GAGCCAAGGA 
ACAGTATCAT ACAAGGGCTT GCGAGGGCGC AATCAGTACA CGTTCGTTTC GGACTGGTTC 
CGGATGATGC TGGACGATCA CACGAACGAT ATGTGTAAGA CACCGAATTT GCAAACCATG 
TCTTCTATAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

cuff cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報