pyr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pyr
Entrez Gene ID	36255
Full Name	pyramus
Synonyms	CG13194,DFGF8-2,DmPyr,Dmel\CG13194,FGF8-like2,Pyr,fgf8-like
General protein information	Preferred Names pyramus Names CG13194 gene product from transcript CG13194-RB CG13194-PB pyr-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48C2-48C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103805.2	NP_001097275.1	pyramus

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that Pyramus (Pyr) and Thisbe (Ths) signals play an obligatory role for the survival of the migrating cells.
Title: The FGF8-related signals Pyramus and Thisbe promote pathfinding, substrate adhesion, and survival of migrating longitudinal gut muscle founder cells.

Data show that FGF signaling regulates directional movement of caudal visceral mesoderm cells and that presentation of both FGF ligands, Pyramus and Thisbe, together is most effective at attracting cells.
Title: Synchronous and symmetric migration of Drosophila caudal visceral mesoderm cells requires dual input by two FGF ligands.

Ths and Pyr proteins are secreted from S2 cells as N-terminal fragments as a result of intracellular proteolytic cleavage; the FGF-domain is contained within the secreted ligand portion, and is functions in the embryo when ectopically expressed.
Title: Analysis of Thisbe and Pyramus functional domains reveals evidence for cleavage of Drosophila FGFs.

pyr and ths single mutants display defects in gastrulation and mesoderm differentiation.
Title: Differential and overlapping functions of two closely related Drosophila FGF8-like growth factors in mesoderm development.

The following pyr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pyr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09639	NM_001103805.2 Latest version!	Drosophila melanogaster pyramus (pyr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pyr ( NM_001103805.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097275.1 Drosophila melanogaster pyramus (pyr), mRNA.

Price & Availability↑

CloneID	OFa09639
Clone ID Related Accession (Same CDS sequence)	NM_001103805.2
Accession Version	NM_001103805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyramus
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103805.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCACA AGTTCATGCC CAATGTCAGG ACTTATATTA TACTGATGAG TTTTGTGGCT 
GCTATTGCGT GGTCAGCAAG CGCCGCGAAA AATGTTTTAA CATTGAGCAA ACACAGCGAA 
CTTGCGCTGC ACATCAATTC GCACGGCAAA GTGACCGCCG AGAACATACT GCGGACATAT 
CAATACTTCA ATATGGAAGC TGTCAACGAT GTGTTCAGCC TGGATTTCAA AATCACCATC 
TATTCGGCGG ATGTAGACTA CTATTTGTGC TTCCATCACG GCAGACTGGT TGGAAAGAGA 
AACGCCACAA AGGAGTGCCA CTTTAAGGAG GGAATTTTCA TGGGCAATCA GAACTTTAGT 
AGCGCCTACA ATCCAGTGCT GCGTGTGGGT TTCAAGGGAA ATTTCAAGCC CATTGGCTTG 
AATGATTTTT CCAAACCGAA AGTGATGAAG AAGGCCATAT TGTTTTTCTT TCCTTTGAAT 
GAGGAGAAAT TCAATTTACA TTTGGCTGAA GTGCTGAGCA GTAGCACCAC TACCACCACG 
ACTACAACAA CAACTACATC AACAACAACA ACAACGACAA CACCACCACC CAGTACCACC 
ACAACAACAA CAACCACAAC AACCACACCA GCTAGCCCAG TGGCGGTCAC AAAACGGACG 
CGTTCCAAAT CGCGTCGCCC AACAAGTATT AATAGCAATA GTAGCAACAG CAATATCCCT 
GACAAAATCA GCAGACACAA CAGTAATAGC CTTAAGTCCT ACAACCAAGC CAATAGCAAC 
AACAACAACA ACAACAACAA CAACAACAGC AGTAACAATA ATGAGTTGGA TCTGCAGCAA 
CAACAGGTGA TCCTGCAGAG AAATCGCAAA CTCCATCGCC GCCACAGGTT GCAACAAAAG 
AAGCGGCAGC TGCAACTGCA GCAGCAACAA CGCCGTCGCC AGCGGCAGTA CGGCACAATG 
GGAGTGATGG CCCAACCGCA GCCCAAAAAC CTGGTGGTGC GTCATCATCA CAACAATGCC 
ACCATTATGG AACGGCGTAG ACGTCGCCGC TTGGAGCGGC AGCAGCACAA GTTGCAGCGC 
GAGTTGTGGG AGCGCGAGCA GCGCGAGGCG GGCGACAAGG AGCGGGAACG CGAGCGGGTG 
GAGCATCTGC CCAAGGCCAC CTCCTCGGCC TCGTCCTCCT CCTCCTCCTC GTCGACGGCC 
ACCTCGACCG CTCTGACCGC CACGGCCGCC TCGTTGGCAC CCAGCGCCTT CAATTTGGTG 
GCCATCATGG CGGCCAGTCG TCGCAAGCGG GACAGGCGAA AACGCTCGGC GGGAGCAACA 
GGTGCTGGTG CGGGCGCAAC AGGTGCGGGG GCAACAAGGG GCGGCGGCAG CAGCAGCAGT 
CCGCCCGATG CTGATATGCA ACTGGTTGAG CTGCCCTCGC AGCGATTGCA GCAGCAGGAT 
GAGCAGCACC CACAAAACGA ATACTCAAAA CCCTATGTTA ATAGTAACTT AAACTTAGAT 
CTAGACCTAA ACGTAAACGT AAAGCAACTA ATTGATAGTA GTAGCGTTAG TGTTAATCAT 
GCAATACCTG CCTTGCCAAA AAACTACAAC TACTTGTACA GTAACGAGCA TTATAATTTG 
AATACTAAAA GTAGCACGAC TGATTCTAGT AGTTTAGACG ATAGCACTAA GTTCGATAGT 
CCAAATAGCC ATAGCCATAG CCACAGCGCG TTCAATCAGA ATATTGACAA TAATCTTAAG 
CAATTAACCG ATCGAATTGA ACTTGCCTCA GCTGAAAGCC AAGTTGAGAC AACAGCGAAC 
CCCGAAGGTG AGACAGAGAC AGTTACTACC GAAACTGTAA TGCAGAATCG CAATCATATT 
GATGCTAATA ATATAATCGA CGATAGCTAT CCGAACGACG AGGAGCACGA AGAGCTGATT 
CTAAAGAAAC TTCTTCGTGG CCTCAGGTTG CAACAGCAAC ACCAACAGCA GCAACTGCAA 
CAGCAGCAGC AACAACCTAA TATTGAAAAC CCTAACGAAA ATATTAATGT TGTTAACAAT 
AATCAGAATC ATAACGATGG TGGTCTGCTG ACAAACAATA ACCATAATAG TAATTACAAT 
GAGCAATTTG CGAATGACGA TGAAACGATA CGAATTCAAA ATAATAAATA TGAAACACAT 
ATAGTACAAT ACAAACATGC GGTATTTATT TGGAAAATAT TGGGGGTATC TAGGAGATAT 
GATAATCCTA TAATCATTGC TTGGCAATTT GCATGTCACT GTGGTAAGTA TTTTGTTAGC 
TTGTATTATA TAGATTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097275.1
CDS	867..3167
Translation	MFHKFMPNVRTYIILMSFVAAIAWSASAAKNVLTLSKHSELALHINSHGKVTAENILRTYQYFNMEAVNDVFSLDFKITIYSADVDYYLCFHHGRLVGKRNATKECHFKEGIFMGNQNFSSAYNPVLRVGFKGNFKPIGLNDFSKPKVMKKAILFFFPLNEEKFNLHLAEVLSSSTTTTTTTTTTTSTTTTTTTPPPSTTTTTTTTTTTPASPVAVTKRTRSKSRRPTSINSNSSNSNIPDKISRHNSNSLKSYNQANSNNNNNNNNNNSSNNNELDLQQQQVILQRNRKLHRRHRLQQKKRQLQLQQQQRRRQRQYGTMGVMAQPQPKNLVVRHHHNNATIMERRRRRRLERQQHKLQRELWEREQREAGDKERERERVEHLPKATSSASSSSSSSSTATSTALTATAASLAPSAFNLVAIMAASRRKRDRRKRSAGATGAGAGATGAGATRGGGSSSSPPDADMQLVELPSQRLQQQDEQHPQNEYSKPYVNSNLNLDLDLNVNVKQLIDSSSVSVNHAIPALPKNYNYLYSNEHYNLNTKSSTTDSSSLDDSTKFDSPNSHSHSHSAFNQNIDNNLKQLTDRIELASAESQVETTANPEGETETVTTETVMQNRNHIDANNIIDDSYPNDEEHEELILKKLLRGLRLQQQHQQQQLQQQQQQPNIENPNENINVVNNNQNHNDGGLLTNNNHNSNYNEQFANDDETIRIQNNKYETHIVQYKHAVFIWKILGVSRRYDNPIIIAWQFACHCGKYFVSLYYIDL

Target ORF information:

RefSeq Version	NM_001103805.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyramus (pyr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103805.2

ORF Insert Sequence:

ATGTTCCACA AGTTCATGCC CAATGTCAGG ACTTATATTA TACTGATGAG TTTTGTGGCT 
GCTATTGCGT GGTCAGCAAG CGCCGCGAAA AATGTTTTAA CATTGAGCAA ACACAGCGAA 
CTTGCGCTGC ACATCAATTC GCACGGCAAA GTGACCGCCG AGAACATACT GCGGACATAT 
CAATACTTCA ATATGGAAGC TGTCAACGAT GTGTTCAGCC TGGATTTCAA AATCACCATC 
TATTCGGCGG ATGTAGACTA CTATTTGTGC TTCCATCACG GCAGACTGGT TGGAAAGAGA 
AACGCCACAA AGGAGTGCCA CTTTAAGGAG GGAATTTTCA TGGGCAATCA GAACTTTAGT 
AGCGCCTACA ATCCAGTGCT GCGTGTGGGT TTCAAGGGAA ATTTCAAGCC CATTGGCTTG 
AATGATTTTT CCAAACCGAA AGTGATGAAG AAGGCCATAT TGTTTTTCTT TCCTTTGAAT 
GAGGAGAAAT TCAATTTACA TTTGGCTGAA GTGCTGAGCA GTAGCACCAC TACCACCACG 
ACTACAACAA CAACTACATC AACAACAACA ACAACGACAA CACCACCACC CAGTACCACC 
ACAACAACAA CAACCACAAC AACCACACCA GCTAGCCCAG TGGCGGTCAC AAAACGGACG 
CGTTCCAAAT CGCGTCGCCC AACAAGTATT AATAGCAATA GTAGCAACAG CAATATCCCT 
GACAAAATCA GCAGACACAA CAGTAATAGC CTTAAGTCCT ACAACCAAGC CAATAGCAAC 
AACAACAACA ACAACAACAA CAACAACAGC AGTAACAATA ATGAGTTGGA TCTGCAGCAA 
CAACAGGTGA TCCTGCAGAG AAATCGCAAA CTCCATCGCC GCCACAGGTT GCAACAAAAG 
AAGCGGCAGC TGCAACTGCA GCAGCAACAA CGCCGTCGCC AGCGGCAGTA CGGCACAATG 
GGAGTGATGG CCCAACCGCA GCCCAAAAAC CTGGTGGTGC GTCATCATCA CAACAATGCC 
ACCATTATGG AACGGCGTAG ACGTCGCCGC TTGGAGCGGC AGCAGCACAA GTTGCAGCGC 
GAGTTGTGGG AGCGCGAGCA GCGCGAGGCG GGCGACAAGG AGCGGGAACG CGAGCGGGTG 
GAGCATCTGC CCAAGGCCAC CTCCTCGGCC TCGTCCTCCT CCTCCTCCTC GTCGACGGCC 
ACCTCGACCG CTCTGACCGC CACGGCCGCC TCGTTGGCAC CCAGCGCCTT CAATTTGGTG 
GCCATCATGG CGGCCAGTCG TCGCAAGCGG GACAGGCGAA AACGCTCGGC GGGAGCAACA 
GGTGCTGGTG CGGGCGCAAC AGGTGCGGGG GCAACAAGGG GCGGCGGCAG CAGCAGCAGT 
CCGCCCGATG CTGATATGCA ACTGGTTGAG CTGCCCTCGC AGCGATTGCA GCAGCAGGAT 
GAGCAGCACC CACAAAACGA ATACTCAAAA CCCTATGTTA ATAGTAACTT AAACTTAGAT 
CTAGACCTAA ACGTAAACGT AAAGCAACTA ATTGATAGTA GTAGCGTTAG TGTTAATCAT 
GCAATACCTG CCTTGCCAAA AAACTACAAC TACTTGTACA GTAACGAGCA TTATAATTTG 
AATACTAAAA GTAGCACGAC TGATTCTAGT AGTTTAGACG ATAGCACTAA GTTCGATAGT 
CCAAATAGCC ATAGCCATAG CCACAGCGCG TTCAATCAGA ATATTGACAA TAATCTTAAG 
CAATTAACCG ATCGAATTGA ACTTGCCTCA GCTGAAAGCC AAGTTGAGAC AACAGCGAAC 
CCCGAAGGTG AGACAGAGAC AGTTACTACC GAAACTGTAA TGCAGAATCG CAATCATATT 
GATGCTAATA ATATAATCGA CGATAGCTAT CCGAACGACG AGGAGCACGA AGAGCTGATT 
CTAAAGAAAC TTCTTCGTGG CCTCAGGTTG CAACAGCAAC ACCAACAGCA GCAACTGCAA 
CAGCAGCAGC AACAACCTAA TATTGAAAAC CCTAACGAAA ATATTAATGT TGTTAACAAT 
AATCAGAATC ATAACGATGG TGGTCTGCTG ACAAACAATA ACCATAATAG TAATTACAAT 
GAGCAATTTG CGAATGACGA TGAAACGATA CGAATTCAAA ATAATAAATA TGAAACACAT 
ATAGTACAAT ACAAACATGC GGTATTTATT TGGAAAATAT TGGGGGTATC TAGGAGATAT 
GATAATCCTA TAATCATTGC TTGGCAATTT GCATGTCACT GTGGTAAGTA TTTTGTTAGC 
TTGTATTATA TAGATTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pyr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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