E(Pc) cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	E(Pc)
Entrez Gene ID	36238
Full Name	Enhancer of Polycomb
Synonyms	CG7776,Dmel\CG7776,E(PC),GH05739,GH14582,III,anon-48Ac,e(Pc),e(pc),l(2)28-28-12
General protein information	Preferred Names Enhancer of Polycomb Names CG7776 gene product from transcript CG7776-RE CG7776-PA CG7776-PB CG7776-PC CG7776-PD CG7776-PE E(Pc)-PA E(Pc)-PB E(Pc)-PC E(Pc)-PD E(Pc)-PE enhancer of polycomb transcript group III
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47F13-47F14\|2-61.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273966.2	NP_001260895.1	enhancer of polycomb, isoform C
NM_001273967.1	NP_001260896.1	enhancer of polycomb, isoform D
NM_001273968.1	NP_001260897.1	enhancer of polycomb, isoform E
NM_078974.3	NP_523698.2	enhancer of polycomb, isoform A
NM_165836.3	NP_725054.1	enhancer of polycomb, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

esc and E(Pc) mutants enhance homologous recombination by modulating the histone acetylation status of histone H4 at the double-strand break
Title: Mutations in the extra sex combs and Enhancer of Polycomb genes increase homologous recombination in somatic cells of Drosophila melanogaster.

The following E(Pc) gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E(Pc) cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09616	NM_165836.3 Latest version!	Drosophila melanogaster enhancer of polycomb, transcript variant B (E(Pc)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa09616	NM_078974.3 Latest version!	Drosophila melanogaster enhancer of polycomb, transcript variant A (E(Pc)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa12167	NM_001273968.1 Latest version!	Drosophila melanogaster enhancer of polycomb, transcript variant E (E(Pc)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa12168	NM_001273967.1 Latest version!	Drosophila melanogaster enhancer of polycomb, transcript variant D (E(Pc)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa12169	NM_001273966.2 Latest version!	Drosophila melanogaster enhancer of polycomb, transcript variant C (E(Pc)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E(Pc) ( NM_078974.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523698.2 Drosophila melanogaster enhancer of polycomb, transcript variant A (E(Pc)), mRNA.

Price & Availability↑

CloneID	OFa09616
Clone ID Related Accession (Same CDS sequence)	NM_078974.3 , NM_165836.3
Accession Version	NM_078974.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of polycomb, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_078974.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GACCCGCGTA TAATCCATTG TATACGAATC AATACTCACA AGGGGCGGCG 
GCGGTGGGAA CGGCTGGTGC CATTCTAGCC GCCCTGCCCA CGGGTCTGCT GCCGGGCGGT 
GCTGGAGCGG CAGGCAATGC AAATGTCTTC GGCTCAGCGT CCCAGTACCT GAACTCGTCG 
AATCTGACAA TGGATGCCAT CAGTGGCAGT GGGAATGGAA ACAGCAGTCG CAAGGAGAAG 
CGTCCATACA AGAAGCGCAA GCACAAATTG CCCCGCGACA AGCAGCAGCA CCAGCAGCAG 
CAAACGCAGC AGCACCAGCA ACAACAGTAT TTGCCGGCGT CTGGTGGCGC CGGAGTCTCG 
CCCGCGCACC ACCTGCCCCA CCATCTGCAC CACCTCAGCA GGCAGCAGAG TGCCTCGCCG 
GCAGCCAACG ACTCGATTGC GGACAGCGAG GAGGAGGACT ACTTTGGCGC CGGTGCCCAG 
AATGGAAACA AACTGGGCTC CGAGAGCGAG GAGGAGACGC CGTTTGCATT TAGGCGCAGA 
TCAAGCTGTG TTTACCTACC GACTCGACAA CGAGATGGCC GCTATCCGTG GGACTCAGCC 
GACGAGGATA TGGCGCCCAG TGGTGCTTGT TCGGATGCTA AATACCGTTA CACACTGACC 
TCCCTCAATT ATCCCAGACC ACGCTGCATT GGCTTCGCAC GTCGTCGATT GGGTCGTGGC 
GGTCGCATCC TGCTGGACCG GGCCACAACG AATTTCGACG ACTTTTGGTC GCAGCTTGAC 
TACACGGTGA TGGAAAGCGT GACGGTCAAC AAATGTAGGG ATAAAATCAA GCAACGGGAT 
CCCGATCAGC TGTGCAAGCC CAGCTCACCG CCCACGGTGG TAGATCTGGT TCCCGCTGGA 
AAGCCCGTCG ATGGTGAGCA TCCTGTTCCT GAACTGATAA AGGAGGAGCC GTCAAGCGAT 
ACAGAAAAGC CTGTTGCTGC GACGGAATCT GGGCGGGAGA GCAAAGAACG GGAGCTTGAG 
GAGAAATTTG AGAACGATGT AGATGATGAA TTCGTAGCCA GTGACGATGA GAATGTGTCA 
CGGCTTGGAA TCTACAGTTC AGCGGTTACG TTACCCCAAA GTTGTGCTGA TCTTCGCCAG 
AAACGTCGCC GCCTGCGTCG CAAGAAGCAA CAGTTGCGAG AACTTAATGC CGCAAAGCGT 
CTAAAGAGGT CTTCGGAAGC GGTGGCAACC GCTGAAGATG TTCAACTGGA GGACGAGGAT 
ACTAAACCAG ATCTGCGTCC ATTACCCAGG GCTTACCTTC AGCGTTTGGC ATTGATTCTG 
GGACAAAAAC TAAAGGTGGA AGAGGATGAT AACACGGAAG TTTCCGAGAA TGTGGCGCCA 
GAGTTGCTGG AAATGAATGA TCCGGAGCAG CTGCAGCCCC CGCGAGCCAA TCATCAGCAA 
CACCACCGTT CCAACCACAA CAACAACAAC AATACCGTGT TGAACAACAA CAATAGTAGT 
AGTAGTAACG TTATGAATAA GGATGTTAGC ATTAGCGAAA AAATCAATGT GATAAAGCAG 
GAGGCGGAGG AGCTCGTCGG CGGCAAATGC GAGGATCAAC CGGTAGCTTC CACCTCGGCG 
GCAGCGGCCG CCGCTCGTGC CGCTGAAGCA GCCGCTGCAG CGGAGGCAGC CTCATCGACA 
ACCACTCTTG CGGCACCGAA TCCGGGTCCA TCGCTGGAGG AGGTGGCGAA GACGATAAAG 
CGCGAGATGA TCGATGCCGA CGACTCCAAC GAGCCCCTGA GCAGCATTCG TACAGCAGCT 
GTCATGCTGT CCACGCAGAC CTCTGTCTCC GAACTCATGG ACGATGAAGC TGAGGACGAT 
GTCAATCTCG CCCAGCTGAG CAGTATCATC CGGCATACGG CGGTCAAGAA GGAACTGGAG 
GCCCAGCAGC ACCAGCAACA AGTACAGCAT GAACAGATGC AGGCCGCCAG GAAGATGGAA 
GAGGATGAAG CCGCTCCCTG TCTGAACCAG CTGCCTGACG TGATTCGCTT GCGAAATATC 
CGCAACAGCC AAGTGCATGC GAGGCCGAAG GACCTGTTTG TACAAGCTCC TGTTGATGAA 
GAATTCAATG CAGACTACAT GGGTGGACTG TCGCCCACTT CCAGCCAGCG GCTAGACATC 
TGCAATGAGC TGCTTTCGGA GATCCGGCGC GATTGGCTAC ACTTCCGGCC CAAAACTCCC 
ACAGATGAGC TGTCCGATAG CCAAGATTGC GAAGTGGGTA AGACGTGTGA TAGTCACGCT 
GTCGACTGGA CGCAGGACAC GCCCATAAGT GTGGAACTCA ATCGCTTAGG AAAGCGAGAA 
GCAGAGGAAA GCGATTCTAG CTCGTACTTT TTGAGCAGTG GCTTTAAATA CAGGGATCTC 
GAGTCAGATG CCCAATTGCT GCAAACGGTC CAAGCTCAGG ACTACGCTCG AGGCAGCAGC 
AAGAGTCCAA ACTGCTCTGG GTCAGGCTCA GGATCTGCGC TAGAGTTTAA TCTCAGTGGT 
GGTGACTCCC TAAACGATAT CAACAACTTG TTGGGCTGTG ACGACGAGGA CGACAACCAC 
GAGCATATGC TAGACAACAT CCTGCAGGAG TGCGCCATGG ATGATCCCAA GGCATTGAAC 
CAAGCTACTA ATTTTTGGAA CGGTATCCTA GATGGCGAGG CAGCCGTGGA CGAAGTGGAA 
ATGGCCGATC AGCTGTTGGA TTGTATAGAC GAGAAGAAAT CTAAGGTGGG TGCAGCCGAT 
ACATCGAAGC GCGCGGGTCG CAACCGCAAG GCAAGATTGA TCCAAGCTCC AGTGGGTAGC 
TCCGCATTTA CTGTGTGTCC CACGGCGGAG ACTCTGAAGG AACGTGAACT TTTCTTTAGT 
CAAGCTCCGC CTGTTGAAGT CCCGCCCAAA GAGAAAACGC CAGCAGAAGT TGAACTGCCG 
GAAGTTGTGG TCAAGGAAAC AGCGAATTTG CCTGTGAAGG TTGAGGCGGT TGAGAAACCC 
CAGTCGGAGG TTCTTCCCGT TGCCCCACAG CCGCAACAGC TTCAGCCGCA GGCTATTACT 
TTGCCCACGA CGCAGCTGAT CAACATACCC GCCCAGATAA GCACGCAAAA GCAGCATCAG 
CAGCCACAGG TGACGCAATT GCTCAATCAG TTTGCCAACG CTGGTCCGCA GATCATCGCC 
CGCACAGAAT ACGGCGGAGG AGCAGCTGTG GGCGATATAG TGACCATAAC CCCGCACACG 
GGGAGCAGTC CACTCCCCAC ACTCACCGCC CAGCAACAAC TGCAGCACCA ATTGGCACAG 
GCTCAACTCC GAAATGTGAC CGTCCAGCAG CGACAGGCGA CACCTCAAAA CCCCAATCAA 
AATCAGAATC CCCAGCAGCA GTTACTCTTC CAAATGCAGC GGGATCCCTC GCGCTCCCTG 
ACGCCCCTAC AAGCTGCCGG CGCTGGCGCC ACTGGCAACC ATTTGATCTA CACAACAAGC 
TGCGGCGAGA TTGTCACAGC AGGAGCTGCA GCTTCTGCGT CTCAGAAGGT CACATATGCC 
ATCCAGAAAG CCGGTGTCTC AAGTGGAGGA GCAACCACCG CCTCAATTGG ACCCAACACA 
GTAATCACGC TGTCCAATGT AAAGGTGCAG AACGATGATA TCTCCTCTGG TGGTAGTGGC 
AACTCAGGAT CCAGTGTGAA CATCATCAAC ACGGCCAGTG GCCAGCAGTT AGCCGTGCAC 
AGCATTGCCG GTATGCAACA GCAGGGCCAG CAACAGGTGC AACAGGTGAC CACCAGTACG 
CCTTTGCTTG TCGCCACACC CACTCGCAAC AATGTGCAGC AGCAACAGCA GCAGTTGGTG 
CAGCAGCAAC CGATTGTTTG GCGGCAGGTG AGCGGAACGA ACACGGCGGT GGCCACCACA 
GCAGTGCTCT CCACCACGGT GGACTCCTCG CCGAAGGCGG TAACCAACCA GATCCTCTGG 
ACCAGCCGGG CCACGCAAAA AAGGCAGCTC AACGGGCCAG AGAACACAGA CATCAACAAA 
CTTCTTCTCA ATCGGAAGTT GCCGCGCAAG CAGCAAATAC AACAACAGCA CCAACAGATT 
CAACTGACAA AGCAGCAGCT TCAACAATTG GTGCAGCATG AGGAGTTGGA TGACTTGGTG 
GCCACATCGA CGGGCAACAG TGGCGATCAG ATGGAAACCG GCGACGGCCT GCAACAACAA 
CAGCAACAGC AGCAACAACA GCAGCAGCTG CAGAAGCTCA GCAAGGTGAT CAAGATGTCG 
CAGTTTCCAC TGCTCGTGTC GGATGTAAGT CTGCAGCAGC AGCAGTCGGT GTCTGGCCAA 
GCTCAGCAGC AACAATTAAA GCCCAACTCT GCGGCGGCGA TTGCAGCTAA TCACAACTAC 
AGCCTGCACC CTGCCTCAAC GGCGATCATC ACCGCCCAGG CGACACCCCA GCAGGCCAAC 
AAGATCTTCT TGACCAGCAG CAACAGTGGT GGCGGCGGAT CCGCTGGTAA CTTTACCATC 
GCCACTGCCA GCGAACTGCA GGCGGCGGCA GCCGGCCAAA AGCTTCACTA CAAGGAGCTG 
CCCAACTCCA ACCTCAAGCT GAACTTTGTG AGCAGTGCAG GGACGGGTGA GCAAACGCAG 
CAGGTGGGCG CCACAACAGT GCTGCTGAAC AAATCCCAAC AGCAGCAGCA ACAACAGCAG 
CATCAAAAGC TGAAAACGTT GGCCACTACA GGAAATTCTC AAACTGGCCA GGGCGATAAG 
CGAGTGCTGT ACACCAGTCT GCTGAATGTG AAACCGCTGC AGAAGAACAA CAGTGGTCAG 
ATGCAGATGC AACTGGGACC GGGAGGATTG CAACAGGTGC TCCGTGGACG ACTCAACAAT 
TCAGCGATCA TTACTAGAAC GCTGCCGCTG GGCACAACTG TTAACAGTAC CGGCGGTGCT 
GTAGGTCCTA CCACCACGAC TATTAGGCAG TTGGTTACCA CGAATGCGAA CAACGTGAGC 
AGCCAAGATG GCAACCTACT TAGTGCAAAG GAAGTGGGGA AAGCCCTGAC AGTGGAGCAG 
GTGATAGCCA GCGCTAACAA CGGAGGAGTT GTGCTGCCCA CGAGCAACAA CAATAATAAC 
AACAATGGCA GTGGAGCTGG CGGAAATGGA GGACCTTTGA ACAGCGGTGT CAATGCCACA 
GGAGCTGGCG GAGGAGGATC TACAGGCGCG GGTGTTGGGG GAGCTGGTGT GACGTCAACC 
ATCAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523698.2
CDS	1120..7191
Translation	MSKLSFRARHLDPSKQMPIYLAEELPDLPEYSAINRAVPQMPSGMEKEEESEHHLQRAICTGLIIPTPEVLQTDQPFYDAYYPPDYKMPRQMIHMQPLGLDTEVPDYDMDSADEDWLSQQQRLELTELKFEQMMDRLEKSSGQTVVTLNEAKSLLNQDDETSISVYDYWLNKRLKMQHPLILTVKTESRPGASSNNPYLAFRRRTEKMQTRKNRKNDEASYEKMLKLRRDLQRATTILEMVRRREETKRDHLKMTVNIFEKRVEMRDFNGAVYSELNSQYKNTRPAYNPLYTNQYSQGAAAVGTAGAILAALPTGLLPGGAGAAGNANVFGSASQYLNSSNLTMDAISGSGNGNSSRKEKRPYKKRKHKLPRDKQQHQQQQTQQHQQQQYLPASGGAGVSPAHHLPHHLHHLSRQQSASPAANDSIADSEEEDYFGAGAQNGNKLGSESEEETPFAFRRRSSCVYLPTRQRDGRYPWDSADEDMAPSGACSDAKYRYTLTSLNYPRPRCIGFARRRLGRGGRILLDRATTNFDDFWSQLDYTVMESVTVNKCRDKIKQRDPDQLCKPSSPPTVVDLVPAGKPVDGEHPVPELIKEEPSSDTEKPVAATESGRESKERELEEKFENDVDDEFVASDDENVSRLGIYSSAVTLPQSCADLRQKRRRLRRKKQQLRELNAAKRLKRSSEAVATAEDVQLEDEDTKPDLRPLPRAYLQRLALILGQKLKVEEDDNTEVSENVAPELLEMNDPEQLQPPRANHQQHHRSNHNNNNNTVLNNNNSSSSNVMNKDVSISEKINVIKQEAEELVGGKCEDQPVASTSAAAAAARAAEAAAAAEAASSTTTLAAPNPGPSLEEVAKTIKREMIDADDSNEPLSSIRTAAVMLSTQTSVSELMDDEAEDDVNLAQLSSIIRHTAVKKELEAQQHQQQVQHEQMQAARKMEEDEAAPCLNQLPDVIRLRNIRNSQVHARPKDLFVQAPVDEEFNADYMGGLSPTSSQRLDICNELLSEIRRDWLHFRPKTPTDELSDSQDCEVGKTCDSHAVDWTQDTPISVELNRLGKREAEESDSSSYFLSSGFKYRDLESDAQLLQTVQAQDYARGSSKSPNCSGSGSGSALEFNLSGGDSLNDINNLLGCDDEDDNHEHMLDNILQECAMDDPKALNQATNFWNGILDGEAAVDEVEMADQLLDCIDEKKSKVGAADTSKRAGRNRKARLIQAPVGSSAFTVCPTAETLKERELFFSQAPPVEVPPKEKTPAEVELPEVVVKETANLPVKVEAVEKPQSEVLPVAPQPQQLQPQAITLPTTQLINIPAQISTQKQHQQPQVTQLLNQFANAGPQIIARTEYGGGAAVGDIVTITPHTGSSPLPTLTAQQQLQHQLAQAQLRNVTVQQRQATPQNPNQNQNPQQQLLFQMQRDPSRSLTPLQAAGAGATGNHLIYTTSCGEIVTAGAAASASQKVTYAIQKAGVSSGGATTASIGPNTVITLSNVKVQNDDISSGGSGNSGSSVNIINTASGQQLAVHSIAGMQQQGQQQVQQVTTSTPLLVATPTRNNVQQQQQQLVQQQPIVWRQVSGTNTAVATTAVLSTTVDSSPKAVTNQILWTSRATQKRQLNGPENTDINKLLLNRKLPRKQQIQQQHQQIQLTKQQLQQLVQHEELDDLVATSTGNSGDQMETGDGLQQQQQQQQQQQQLQKLSKVIKMSQFPLLVSDVSLQQQQSVSGQAQQQQLKPNSAAAIAANHNYSLHPASTAIITAQATPQQANKIFLTSSNSGGGGSAGNFTIATASELQAAAAGQKLHYKELPNSNLKLNFVSSAGTGEQTQQVGATTVLLNKSQQQQQQQQHQKLKTLATTGNSQTGQGDKRVLYTSLLNVKPLQKNNSGQMQMQLGPGGLQQVLRGRLNNSAIITRTLPLGTTVNSTGGAVGPTTTTIRQLVTTNANNVSSQDGNLLSAKEVGKALTVEQVIASANNGGVVLPTSNNNNNNNGSGAGGNGGPLNSGVNATGAGGGGSTGAGVGGAGVTSTINR

Target ORF information:

RefSeq Version	NM_078974.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of polycomb, transcript variant A (E(Pc)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078974.3

ORF Insert Sequence:

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GACCCGCGTA TAATCCATTG TATACGAATC AATACTCACA AGGGGCGGCG 
GCGGTGGGAA CGGCTGGTGC CATTCTAGCC GCCCTGCCCA CGGGTCTGCT GCCGGGCGGT 
GCTGGAGCGG CAGGCAATGC AAATGTCTTC GGCTCAGCGT CCCAGTACCT GAACTCGTCG 
AATCTGACAA TGGATGCCAT CAGTGGCAGT GGGAATGGAA ACAGCAGTCG CAAGGAGAAG 
CGTCCATACA AGAAGCGCAA GCACAAATTG CCCCGCGACA AGCAGCAGCA CCAGCAGCAG 
CAAACGCAGC AGCACCAGCA ACAACAGTAT TTGCCGGCGT CTGGTGGCGC CGGAGTCTCG 
CCCGCGCACC ACCTGCCCCA CCATCTGCAC CACCTCAGCA GGCAGCAGAG TGCCTCGCCG 
GCAGCCAACG ACTCGATTGC GGACAGCGAG GAGGAGGACT ACTTTGGCGC CGGTGCCCAG 
AATGGAAACA AACTGGGCTC CGAGAGCGAG GAGGAGACGC CGTTTGCATT TAGGCGCAGA 
TCAAGCTGTG TTTACCTACC GACTCGACAA CGAGATGGCC GCTATCCGTG GGACTCAGCC 
GACGAGGATA TGGCGCCCAG TGGTGCTTGT TCGGATGCTA AATACCGTTA CACACTGACC 
TCCCTCAATT ATCCCAGACC ACGCTGCATT GGCTTCGCAC GTCGTCGATT GGGTCGTGGC 
GGTCGCATCC TGCTGGACCG GGCCACAACG AATTTCGACG ACTTTTGGTC GCAGCTTGAC 
TACACGGTGA TGGAAAGCGT GACGGTCAAC AAATGTAGGG ATAAAATCAA GCAACGGGAT 
CCCGATCAGC TGTGCAAGCC CAGCTCACCG CCCACGGTGG TAGATCTGGT TCCCGCTGGA 
AAGCCCGTCG ATGGTGAGCA TCCTGTTCCT GAACTGATAA AGGAGGAGCC GTCAAGCGAT 
ACAGAAAAGC CTGTTGCTGC GACGGAATCT GGGCGGGAGA GCAAAGAACG GGAGCTTGAG 
GAGAAATTTG AGAACGATGT AGATGATGAA TTCGTAGCCA GTGACGATGA GAATGTGTCA 
CGGCTTGGAA TCTACAGTTC AGCGGTTACG TTACCCCAAA GTTGTGCTGA TCTTCGCCAG 
AAACGTCGCC GCCTGCGTCG CAAGAAGCAA CAGTTGCGAG AACTTAATGC CGCAAAGCGT 
CTAAAGAGGT CTTCGGAAGC GGTGGCAACC GCTGAAGATG TTCAACTGGA GGACGAGGAT 
ACTAAACCAG ATCTGCGTCC ATTACCCAGG GCTTACCTTC AGCGTTTGGC ATTGATTCTG 
GGACAAAAAC TAAAGGTGGA AGAGGATGAT AACACGGAAG TTTCCGAGAA TGTGGCGCCA 
GAGTTGCTGG AAATGAATGA TCCGGAGCAG CTGCAGCCCC CGCGAGCCAA TCATCAGCAA 
CACCACCGTT CCAACCACAA CAACAACAAC AATACCGTGT TGAACAACAA CAATAGTAGT 
AGTAGTAACG TTATGAATAA GGATGTTAGC ATTAGCGAAA AAATCAATGT GATAAAGCAG 
GAGGCGGAGG AGCTCGTCGG CGGCAAATGC GAGGATCAAC CGGTAGCTTC CACCTCGGCG 
GCAGCGGCCG CCGCTCGTGC CGCTGAAGCA GCCGCTGCAG CGGAGGCAGC CTCATCGACA 
ACCACTCTTG CGGCACCGAA TCCGGGTCCA TCGCTGGAGG AGGTGGCGAA GACGATAAAG 
CGCGAGATGA TCGATGCCGA CGACTCCAAC GAGCCCCTGA GCAGCATTCG TACAGCAGCT 
GTCATGCTGT CCACGCAGAC CTCTGTCTCC GAACTCATGG ACGATGAAGC TGAGGACGAT 
GTCAATCTCG CCCAGCTGAG CAGTATCATC CGGCATACGG CGGTCAAGAA GGAACTGGAG 
GCCCAGCAGC ACCAGCAACA AGTACAGCAT GAACAGATGC AGGCCGCCAG GAAGATGGAA 
GAGGATGAAG CCGCTCCCTG TCTGAACCAG CTGCCTGACG TGATTCGCTT GCGAAATATC 
CGCAACAGCC AAGTGCATGC GAGGCCGAAG GACCTGTTTG TACAAGCTCC TGTTGATGAA 
GAATTCAATG CAGACTACAT GGGTGGACTG TCGCCCACTT CCAGCCAGCG GCTAGACATC 
TGCAATGAGC TGCTTTCGGA GATCCGGCGC GATTGGCTAC ACTTCCGGCC CAAAACTCCC 
ACAGATGAGC TGTCCGATAG CCAAGATTGC GAAGTGGGTA AGACGTGTGA TAGTCACGCT 
GTCGACTGGA CGCAGGACAC GCCCATAAGT GTGGAACTCA ATCGCTTAGG AAAGCGAGAA 
GCAGAGGAAA GCGATTCTAG CTCGTACTTT TTGAGCAGTG GCTTTAAATA CAGGGATCTC 
GAGTCAGATG CCCAATTGCT GCAAACGGTC CAAGCTCAGG ACTACGCTCG AGGCAGCAGC 
AAGAGTCCAA ACTGCTCTGG GTCAGGCTCA GGATCTGCGC TAGAGTTTAA TCTCAGTGGT 
GGTGACTCCC TAAACGATAT CAACAACTTG TTGGGCTGTG ACGACGAGGA CGACAACCAC 
GAGCATATGC TAGACAACAT CCTGCAGGAG TGCGCCATGG ATGATCCCAA GGCATTGAAC 
CAAGCTACTA ATTTTTGGAA CGGTATCCTA GATGGCGAGG CAGCCGTGGA CGAAGTGGAA 
ATGGCCGATC AGCTGTTGGA TTGTATAGAC GAGAAGAAAT CTAAGGTGGG TGCAGCCGAT 
ACATCGAAGC GCGCGGGTCG CAACCGCAAG GCAAGATTGA TCCAAGCTCC AGTGGGTAGC 
TCCGCATTTA CTGTGTGTCC CACGGCGGAG ACTCTGAAGG AACGTGAACT TTTCTTTAGT 
CAAGCTCCGC CTGTTGAAGT CCCGCCCAAA GAGAAAACGC CAGCAGAAGT TGAACTGCCG 
GAAGTTGTGG TCAAGGAAAC AGCGAATTTG CCTGTGAAGG TTGAGGCGGT TGAGAAACCC 
CAGTCGGAGG TTCTTCCCGT TGCCCCACAG CCGCAACAGC TTCAGCCGCA GGCTATTACT 
TTGCCCACGA CGCAGCTGAT CAACATACCC GCCCAGATAA GCACGCAAAA GCAGCATCAG 
CAGCCACAGG TGACGCAATT GCTCAATCAG TTTGCCAACG CTGGTCCGCA GATCATCGCC 
CGCACAGAAT ACGGCGGAGG AGCAGCTGTG GGCGATATAG TGACCATAAC CCCGCACACG 
GGGAGCAGTC CACTCCCCAC ACTCACCGCC CAGCAACAAC TGCAGCACCA ATTGGCACAG 
GCTCAACTCC GAAATGTGAC CGTCCAGCAG CGACAGGCGA CACCTCAAAA CCCCAATCAA 
AATCAGAATC CCCAGCAGCA GTTACTCTTC CAAATGCAGC GGGATCCCTC GCGCTCCCTG 
ACGCCCCTAC AAGCTGCCGG CGCTGGCGCC ACTGGCAACC ATTTGATCTA CACAACAAGC 
TGCGGCGAGA TTGTCACAGC AGGAGCTGCA GCTTCTGCGT CTCAGAAGGT CACATATGCC 
ATCCAGAAAG CCGGTGTCTC AAGTGGAGGA GCAACCACCG CCTCAATTGG ACCCAACACA 
GTAATCACGC TGTCCAATGT AAAGGTGCAG AACGATGATA TCTCCTCTGG TGGTAGTGGC 
AACTCAGGAT CCAGTGTGAA CATCATCAAC ACGGCCAGTG GCCAGCAGTT AGCCGTGCAC 
AGCATTGCCG GTATGCAACA GCAGGGCCAG CAACAGGTGC AACAGGTGAC CACCAGTACG 
CCTTTGCTTG TCGCCACACC CACTCGCAAC AATGTGCAGC AGCAACAGCA GCAGTTGGTG 
CAGCAGCAAC CGATTGTTTG GCGGCAGGTG AGCGGAACGA ACACGGCGGT GGCCACCACA 
GCAGTGCTCT CCACCACGGT GGACTCCTCG CCGAAGGCGG TAACCAACCA GATCCTCTGG 
ACCAGCCGGG CCACGCAAAA AAGGCAGCTC AACGGGCCAG AGAACACAGA CATCAACAAA 
CTTCTTCTCA ATCGGAAGTT GCCGCGCAAG CAGCAAATAC AACAACAGCA CCAACAGATT 
CAACTGACAA AGCAGCAGCT TCAACAATTG GTGCAGCATG AGGAGTTGGA TGACTTGGTG 
GCCACATCGA CGGGCAACAG TGGCGATCAG ATGGAAACCG GCGACGGCCT GCAACAACAA 
CAGCAACAGC AGCAACAACA GCAGCAGCTG CAGAAGCTCA GCAAGGTGAT CAAGATGTCG 
CAGTTTCCAC TGCTCGTGTC GGATGTAAGT CTGCAGCAGC AGCAGTCGGT GTCTGGCCAA 
GCTCAGCAGC AACAATTAAA GCCCAACTCT GCGGCGGCGA TTGCAGCTAA TCACAACTAC 
AGCCTGCACC CTGCCTCAAC GGCGATCATC ACCGCCCAGG CGACACCCCA GCAGGCCAAC 
AAGATCTTCT TGACCAGCAG CAACAGTGGT GGCGGCGGAT CCGCTGGTAA CTTTACCATC 
GCCACTGCCA GCGAACTGCA GGCGGCGGCA GCCGGCCAAA AGCTTCACTA CAAGGAGCTG 
CCCAACTCCA ACCTCAAGCT GAACTTTGTG AGCAGTGCAG GGACGGGTGA GCAAACGCAG 
CAGGTGGGCG CCACAACAGT GCTGCTGAAC AAATCCCAAC AGCAGCAGCA ACAACAGCAG 
CATCAAAAGC TGAAAACGTT GGCCACTACA GGAAATTCTC AAACTGGCCA GGGCGATAAG 
CGAGTGCTGT ACACCAGTCT GCTGAATGTG AAACCGCTGC AGAAGAACAA CAGTGGTCAG 
ATGCAGATGC AACTGGGACC GGGAGGATTG CAACAGGTGC TCCGTGGACG ACTCAACAAT 
TCAGCGATCA TTACTAGAAC GCTGCCGCTG GGCACAACTG TTAACAGTAC CGGCGGTGCT 
GTAGGTCCTA CCACCACGAC TATTAGGCAG TTGGTTACCA CGAATGCGAA CAACGTGAGC 
AGCCAAGATG GCAACCTACT TAGTGCAAAG GAAGTGGGGA AAGCCCTGAC AGTGGAGCAG 
GTGATAGCCA GCGCTAACAA CGGAGGAGTT GTGCTGCCCA CGAGCAACAA CAATAATAAC 
AACAATGGCA GTGGAGCTGG CGGAAATGGA GGACCTTTGA ACAGCGGTGT CAATGCCACA 
GGAGCTGGCG GAGGAGGATC TACAGGCGCG GGTGTTGGGG GAGCTGGTGT GACGTCAACC 
ATCAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(Pc) ( NM_165836.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725054.1 Drosophila melanogaster enhancer of polycomb, transcript variant B (E(Pc)), mRNA.

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CloneID	OFa09616
Clone ID Related Accession (Same CDS sequence)	NM_078974.3 , NM_165836.3
Accession Version	NM_165836.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of polycomb, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165836.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GACCCGCGTA TAATCCATTG TATACGAATC AATACTCACA AGGGGCGGCG 
GCGGTGGGAA CGGCTGGTGC CATTCTAGCC GCCCTGCCCA CGGGTCTGCT GCCGGGCGGT 
GCTGGAGCGG CAGGCAATGC AAATGTCTTC GGCTCAGCGT CCCAGTACCT GAACTCGTCG 
AATCTGACAA TGGATGCCAT CAGTGGCAGT GGGAATGGAA ACAGCAGTCG CAAGGAGAAG 
CGTCCATACA AGAAGCGCAA GCACAAATTG CCCCGCGACA AGCAGCAGCA CCAGCAGCAG 
CAAACGCAGC AGCACCAGCA ACAACAGTAT TTGCCGGCGT CTGGTGGCGC CGGAGTCTCG 
CCCGCGCACC ACCTGCCCCA CCATCTGCAC CACCTCAGCA GGCAGCAGAG TGCCTCGCCG 
GCAGCCAACG ACTCGATTGC GGACAGCGAG GAGGAGGACT ACTTTGGCGC CGGTGCCCAG 
AATGGAAACA AACTGGGCTC CGAGAGCGAG GAGGAGACGC CGTTTGCATT TAGGCGCAGA 
TCAAGCTGTG TTTACCTACC GACTCGACAA CGAGATGGCC GCTATCCGTG GGACTCAGCC 
GACGAGGATA TGGCGCCCAG TGGTGCTTGT TCGGATGCTA AATACCGTTA CACACTGACC 
TCCCTCAATT ATCCCAGACC ACGCTGCATT GGCTTCGCAC GTCGTCGATT GGGTCGTGGC 
GGTCGCATCC TGCTGGACCG GGCCACAACG AATTTCGACG ACTTTTGGTC GCAGCTTGAC 
TACACGGTGA TGGAAAGCGT GACGGTCAAC AAATGTAGGG ATAAAATCAA GCAACGGGAT 
CCCGATCAGC TGTGCAAGCC CAGCTCACCG CCCACGGTGG TAGATCTGGT TCCCGCTGGA 
AAGCCCGTCG ATGGTGAGCA TCCTGTTCCT GAACTGATAA AGGAGGAGCC GTCAAGCGAT 
ACAGAAAAGC CTGTTGCTGC GACGGAATCT GGGCGGGAGA GCAAAGAACG GGAGCTTGAG 
GAGAAATTTG AGAACGATGT AGATGATGAA TTCGTAGCCA GTGACGATGA GAATGTGTCA 
CGGCTTGGAA TCTACAGTTC AGCGGTTACG TTACCCCAAA GTTGTGCTGA TCTTCGCCAG 
AAACGTCGCC GCCTGCGTCG CAAGAAGCAA CAGTTGCGAG AACTTAATGC CGCAAAGCGT 
CTAAAGAGGT CTTCGGAAGC GGTGGCAACC GCTGAAGATG TTCAACTGGA GGACGAGGAT 
ACTAAACCAG ATCTGCGTCC ATTACCCAGG GCTTACCTTC AGCGTTTGGC ATTGATTCTG 
GGACAAAAAC TAAAGGTGGA AGAGGATGAT AACACGGAAG TTTCCGAGAA TGTGGCGCCA 
GAGTTGCTGG AAATGAATGA TCCGGAGCAG CTGCAGCCCC CGCGAGCCAA TCATCAGCAA 
CACCACCGTT CCAACCACAA CAACAACAAC AATACCGTGT TGAACAACAA CAATAGTAGT 
AGTAGTAACG TTATGAATAA GGATGTTAGC ATTAGCGAAA AAATCAATGT GATAAAGCAG 
GAGGCGGAGG AGCTCGTCGG CGGCAAATGC GAGGATCAAC CGGTAGCTTC CACCTCGGCG 
GCAGCGGCCG CCGCTCGTGC CGCTGAAGCA GCCGCTGCAG CGGAGGCAGC CTCATCGACA 
ACCACTCTTG CGGCACCGAA TCCGGGTCCA TCGCTGGAGG AGGTGGCGAA GACGATAAAG 
CGCGAGATGA TCGATGCCGA CGACTCCAAC GAGCCCCTGA GCAGCATTCG TACAGCAGCT 
GTCATGCTGT CCACGCAGAC CTCTGTCTCC GAACTCATGG ACGATGAAGC TGAGGACGAT 
GTCAATCTCG CCCAGCTGAG CAGTATCATC CGGCATACGG CGGTCAAGAA GGAACTGGAG 
GCCCAGCAGC ACCAGCAACA AGTACAGCAT GAACAGATGC AGGCCGCCAG GAAGATGGAA 
GAGGATGAAG CCGCTCCCTG TCTGAACCAG CTGCCTGACG TGATTCGCTT GCGAAATATC 
CGCAACAGCC AAGTGCATGC GAGGCCGAAG GACCTGTTTG TACAAGCTCC TGTTGATGAA 
GAATTCAATG CAGACTACAT GGGTGGACTG TCGCCCACTT CCAGCCAGCG GCTAGACATC 
TGCAATGAGC TGCTTTCGGA GATCCGGCGC GATTGGCTAC ACTTCCGGCC CAAAACTCCC 
ACAGATGAGC TGTCCGATAG CCAAGATTGC GAAGTGGGTA AGACGTGTGA TAGTCACGCT 
GTCGACTGGA CGCAGGACAC GCCCATAAGT GTGGAACTCA ATCGCTTAGG AAAGCGAGAA 
GCAGAGGAAA GCGATTCTAG CTCGTACTTT TTGAGCAGTG GCTTTAAATA CAGGGATCTC 
GAGTCAGATG CCCAATTGCT GCAAACGGTC CAAGCTCAGG ACTACGCTCG AGGCAGCAGC 
AAGAGTCCAA ACTGCTCTGG GTCAGGCTCA GGATCTGCGC TAGAGTTTAA TCTCAGTGGT 
GGTGACTCCC TAAACGATAT CAACAACTTG TTGGGCTGTG ACGACGAGGA CGACAACCAC 
GAGCATATGC TAGACAACAT CCTGCAGGAG TGCGCCATGG ATGATCCCAA GGCATTGAAC 
CAAGCTACTA ATTTTTGGAA CGGTATCCTA GATGGCGAGG CAGCCGTGGA CGAAGTGGAA 
ATGGCCGATC AGCTGTTGGA TTGTATAGAC GAGAAGAAAT CTAAGGTGGG TGCAGCCGAT 
ACATCGAAGC GCGCGGGTCG CAACCGCAAG GCAAGATTGA TCCAAGCTCC AGTGGGTAGC 
TCCGCATTTA CTGTGTGTCC CACGGCGGAG ACTCTGAAGG AACGTGAACT TTTCTTTAGT 
CAAGCTCCGC CTGTTGAAGT CCCGCCCAAA GAGAAAACGC CAGCAGAAGT TGAACTGCCG 
GAAGTTGTGG TCAAGGAAAC AGCGAATTTG CCTGTGAAGG TTGAGGCGGT TGAGAAACCC 
CAGTCGGAGG TTCTTCCCGT TGCCCCACAG CCGCAACAGC TTCAGCCGCA GGCTATTACT 
TTGCCCACGA CGCAGCTGAT CAACATACCC GCCCAGATAA GCACGCAAAA GCAGCATCAG 
CAGCCACAGG TGACGCAATT GCTCAATCAG TTTGCCAACG CTGGTCCGCA GATCATCGCC 
CGCACAGAAT ACGGCGGAGG AGCAGCTGTG GGCGATATAG TGACCATAAC CCCGCACACG 
GGGAGCAGTC CACTCCCCAC ACTCACCGCC CAGCAACAAC TGCAGCACCA ATTGGCACAG 
GCTCAACTCC GAAATGTGAC CGTCCAGCAG CGACAGGCGA CACCTCAAAA CCCCAATCAA 
AATCAGAATC CCCAGCAGCA GTTACTCTTC CAAATGCAGC GGGATCCCTC GCGCTCCCTG 
ACGCCCCTAC AAGCTGCCGG CGCTGGCGCC ACTGGCAACC ATTTGATCTA CACAACAAGC 
TGCGGCGAGA TTGTCACAGC AGGAGCTGCA GCTTCTGCGT CTCAGAAGGT CACATATGCC 
ATCCAGAAAG CCGGTGTCTC AAGTGGAGGA GCAACCACCG CCTCAATTGG ACCCAACACA 
GTAATCACGC TGTCCAATGT AAAGGTGCAG AACGATGATA TCTCCTCTGG TGGTAGTGGC 
AACTCAGGAT CCAGTGTGAA CATCATCAAC ACGGCCAGTG GCCAGCAGTT AGCCGTGCAC 
AGCATTGCCG GTATGCAACA GCAGGGCCAG CAACAGGTGC AACAGGTGAC CACCAGTACG 
CCTTTGCTTG TCGCCACACC CACTCGCAAC AATGTGCAGC AGCAACAGCA GCAGTTGGTG 
CAGCAGCAAC CGATTGTTTG GCGGCAGGTG AGCGGAACGA ACACGGCGGT GGCCACCACA 
GCAGTGCTCT CCACCACGGT GGACTCCTCG CCGAAGGCGG TAACCAACCA GATCCTCTGG 
ACCAGCCGGG CCACGCAAAA AAGGCAGCTC AACGGGCCAG AGAACACAGA CATCAACAAA 
CTTCTTCTCA ATCGGAAGTT GCCGCGCAAG CAGCAAATAC AACAACAGCA CCAACAGATT 
CAACTGACAA AGCAGCAGCT TCAACAATTG GTGCAGCATG AGGAGTTGGA TGACTTGGTG 
GCCACATCGA CGGGCAACAG TGGCGATCAG ATGGAAACCG GCGACGGCCT GCAACAACAA 
CAGCAACAGC AGCAACAACA GCAGCAGCTG CAGAAGCTCA GCAAGGTGAT CAAGATGTCG 
CAGTTTCCAC TGCTCGTGTC GGATGTAAGT CTGCAGCAGC AGCAGTCGGT GTCTGGCCAA 
GCTCAGCAGC AACAATTAAA GCCCAACTCT GCGGCGGCGA TTGCAGCTAA TCACAACTAC 
AGCCTGCACC CTGCCTCAAC GGCGATCATC ACCGCCCAGG CGACACCCCA GCAGGCCAAC 
AAGATCTTCT TGACCAGCAG CAACAGTGGT GGCGGCGGAT CCGCTGGTAA CTTTACCATC 
GCCACTGCCA GCGAACTGCA GGCGGCGGCA GCCGGCCAAA AGCTTCACTA CAAGGAGCTG 
CCCAACTCCA ACCTCAAGCT GAACTTTGTG AGCAGTGCAG GGACGGGTGA GCAAACGCAG 
CAGGTGGGCG CCACAACAGT GCTGCTGAAC AAATCCCAAC AGCAGCAGCA ACAACAGCAG 
CATCAAAAGC TGAAAACGTT GGCCACTACA GGAAATTCTC AAACTGGCCA GGGCGATAAG 
CGAGTGCTGT ACACCAGTCT GCTGAATGTG AAACCGCTGC AGAAGAACAA CAGTGGTCAG 
ATGCAGATGC AACTGGGACC GGGAGGATTG CAACAGGTGC TCCGTGGACG ACTCAACAAT 
TCAGCGATCA TTACTAGAAC GCTGCCGCTG GGCACAACTG TTAACAGTAC CGGCGGTGCT 
GTAGGTCCTA CCACCACGAC TATTAGGCAG TTGGTTACCA CGAATGCGAA CAACGTGAGC 
AGCCAAGATG GCAACCTACT TAGTGCAAAG GAAGTGGGGA AAGCCCTGAC AGTGGAGCAG 
GTGATAGCCA GCGCTAACAA CGGAGGAGTT GTGCTGCCCA CGAGCAACAA CAATAATAAC 
AACAATGGCA GTGGAGCTGG CGGAAATGGA GGACCTTTGA ACAGCGGTGT CAATGCCACA 
GGAGCTGGCG GAGGAGGATC TACAGGCGCG GGTGTTGGGG GAGCTGGTGT GACGTCAACC 
ATCAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725054.1
CDS	1037..7108
Translation	MSKLSFRARHLDPSKQMPIYLAEELPDLPEYSAINRAVPQMPSGMEKEEESEHHLQRAICTGLIIPTPEVLQTDQPFYDAYYPPDYKMPRQMIHMQPLGLDTEVPDYDMDSADEDWLSQQQRLELTELKFEQMMDRLEKSSGQTVVTLNEAKSLLNQDDETSISVYDYWLNKRLKMQHPLILTVKTESRPGASSNNPYLAFRRRTEKMQTRKNRKNDEASYEKMLKLRRDLQRATTILEMVRRREETKRDHLKMTVNIFEKRVEMRDFNGAVYSELNSQYKNTRPAYNPLYTNQYSQGAAAVGTAGAILAALPTGLLPGGAGAAGNANVFGSASQYLNSSNLTMDAISGSGNGNSSRKEKRPYKKRKHKLPRDKQQHQQQQTQQHQQQQYLPASGGAGVSPAHHLPHHLHHLSRQQSASPAANDSIADSEEEDYFGAGAQNGNKLGSESEEETPFAFRRRSSCVYLPTRQRDGRYPWDSADEDMAPSGACSDAKYRYTLTSLNYPRPRCIGFARRRLGRGGRILLDRATTNFDDFWSQLDYTVMESVTVNKCRDKIKQRDPDQLCKPSSPPTVVDLVPAGKPVDGEHPVPELIKEEPSSDTEKPVAATESGRESKERELEEKFENDVDDEFVASDDENVSRLGIYSSAVTLPQSCADLRQKRRRLRRKKQQLRELNAAKRLKRSSEAVATAEDVQLEDEDTKPDLRPLPRAYLQRLALILGQKLKVEEDDNTEVSENVAPELLEMNDPEQLQPPRANHQQHHRSNHNNNNNTVLNNNNSSSSNVMNKDVSISEKINVIKQEAEELVGGKCEDQPVASTSAAAAAARAAEAAAAAEAASSTTTLAAPNPGPSLEEVAKTIKREMIDADDSNEPLSSIRTAAVMLSTQTSVSELMDDEAEDDVNLAQLSSIIRHTAVKKELEAQQHQQQVQHEQMQAARKMEEDEAAPCLNQLPDVIRLRNIRNSQVHARPKDLFVQAPVDEEFNADYMGGLSPTSSQRLDICNELLSEIRRDWLHFRPKTPTDELSDSQDCEVGKTCDSHAVDWTQDTPISVELNRLGKREAEESDSSSYFLSSGFKYRDLESDAQLLQTVQAQDYARGSSKSPNCSGSGSGSALEFNLSGGDSLNDINNLLGCDDEDDNHEHMLDNILQECAMDDPKALNQATNFWNGILDGEAAVDEVEMADQLLDCIDEKKSKVGAADTSKRAGRNRKARLIQAPVGSSAFTVCPTAETLKERELFFSQAPPVEVPPKEKTPAEVELPEVVVKETANLPVKVEAVEKPQSEVLPVAPQPQQLQPQAITLPTTQLINIPAQISTQKQHQQPQVTQLLNQFANAGPQIIARTEYGGGAAVGDIVTITPHTGSSPLPTLTAQQQLQHQLAQAQLRNVTVQQRQATPQNPNQNQNPQQQLLFQMQRDPSRSLTPLQAAGAGATGNHLIYTTSCGEIVTAGAAASASQKVTYAIQKAGVSSGGATTASIGPNTVITLSNVKVQNDDISSGGSGNSGSSVNIINTASGQQLAVHSIAGMQQQGQQQVQQVTTSTPLLVATPTRNNVQQQQQQLVQQQPIVWRQVSGTNTAVATTAVLSTTVDSSPKAVTNQILWTSRATQKRQLNGPENTDINKLLLNRKLPRKQQIQQQHQQIQLTKQQLQQLVQHEELDDLVATSTGNSGDQMETGDGLQQQQQQQQQQQQLQKLSKVIKMSQFPLLVSDVSLQQQQSVSGQAQQQQLKPNSAAAIAANHNYSLHPASTAIITAQATPQQANKIFLTSSNSGGGGSAGNFTIATASELQAAAAGQKLHYKELPNSNLKLNFVSSAGTGEQTQQVGATTVLLNKSQQQQQQQQHQKLKTLATTGNSQTGQGDKRVLYTSLLNVKPLQKNNSGQMQMQLGPGGLQQVLRGRLNNSAIITRTLPLGTTVNSTGGAVGPTTTTIRQLVTTNANNVSSQDGNLLSAKEVGKALTVEQVIASANNGGVVLPTSNNNNNNNGSGAGGNGGPLNSGVNATGAGGGGSTGAGVGGAGVTSTINR

Target ORF information:

RefSeq Version	NM_165836.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of polycomb, transcript variant B (E(Pc)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165836.3

ORF Insert Sequence:

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GACCCGCGTA TAATCCATTG TATACGAATC AATACTCACA AGGGGCGGCG 
GCGGTGGGAA CGGCTGGTGC CATTCTAGCC GCCCTGCCCA CGGGTCTGCT GCCGGGCGGT 
GCTGGAGCGG CAGGCAATGC AAATGTCTTC GGCTCAGCGT CCCAGTACCT GAACTCGTCG 
AATCTGACAA TGGATGCCAT CAGTGGCAGT GGGAATGGAA ACAGCAGTCG CAAGGAGAAG 
CGTCCATACA AGAAGCGCAA GCACAAATTG CCCCGCGACA AGCAGCAGCA CCAGCAGCAG 
CAAACGCAGC AGCACCAGCA ACAACAGTAT TTGCCGGCGT CTGGTGGCGC CGGAGTCTCG 
CCCGCGCACC ACCTGCCCCA CCATCTGCAC CACCTCAGCA GGCAGCAGAG TGCCTCGCCG 
GCAGCCAACG ACTCGATTGC GGACAGCGAG GAGGAGGACT ACTTTGGCGC CGGTGCCCAG 
AATGGAAACA AACTGGGCTC CGAGAGCGAG GAGGAGACGC CGTTTGCATT TAGGCGCAGA 
TCAAGCTGTG TTTACCTACC GACTCGACAA CGAGATGGCC GCTATCCGTG GGACTCAGCC 
GACGAGGATA TGGCGCCCAG TGGTGCTTGT TCGGATGCTA AATACCGTTA CACACTGACC 
TCCCTCAATT ATCCCAGACC ACGCTGCATT GGCTTCGCAC GTCGTCGATT GGGTCGTGGC 
GGTCGCATCC TGCTGGACCG GGCCACAACG AATTTCGACG ACTTTTGGTC GCAGCTTGAC 
TACACGGTGA TGGAAAGCGT GACGGTCAAC AAATGTAGGG ATAAAATCAA GCAACGGGAT 
CCCGATCAGC TGTGCAAGCC CAGCTCACCG CCCACGGTGG TAGATCTGGT TCCCGCTGGA 
AAGCCCGTCG ATGGTGAGCA TCCTGTTCCT GAACTGATAA AGGAGGAGCC GTCAAGCGAT 
ACAGAAAAGC CTGTTGCTGC GACGGAATCT GGGCGGGAGA GCAAAGAACG GGAGCTTGAG 
GAGAAATTTG AGAACGATGT AGATGATGAA TTCGTAGCCA GTGACGATGA GAATGTGTCA 
CGGCTTGGAA TCTACAGTTC AGCGGTTACG TTACCCCAAA GTTGTGCTGA TCTTCGCCAG 
AAACGTCGCC GCCTGCGTCG CAAGAAGCAA CAGTTGCGAG AACTTAATGC CGCAAAGCGT 
CTAAAGAGGT CTTCGGAAGC GGTGGCAACC GCTGAAGATG TTCAACTGGA GGACGAGGAT 
ACTAAACCAG ATCTGCGTCC ATTACCCAGG GCTTACCTTC AGCGTTTGGC ATTGATTCTG 
GGACAAAAAC TAAAGGTGGA AGAGGATGAT AACACGGAAG TTTCCGAGAA TGTGGCGCCA 
GAGTTGCTGG AAATGAATGA TCCGGAGCAG CTGCAGCCCC CGCGAGCCAA TCATCAGCAA 
CACCACCGTT CCAACCACAA CAACAACAAC AATACCGTGT TGAACAACAA CAATAGTAGT 
AGTAGTAACG TTATGAATAA GGATGTTAGC ATTAGCGAAA AAATCAATGT GATAAAGCAG 
GAGGCGGAGG AGCTCGTCGG CGGCAAATGC GAGGATCAAC CGGTAGCTTC CACCTCGGCG 
GCAGCGGCCG CCGCTCGTGC CGCTGAAGCA GCCGCTGCAG CGGAGGCAGC CTCATCGACA 
ACCACTCTTG CGGCACCGAA TCCGGGTCCA TCGCTGGAGG AGGTGGCGAA GACGATAAAG 
CGCGAGATGA TCGATGCCGA CGACTCCAAC GAGCCCCTGA GCAGCATTCG TACAGCAGCT 
GTCATGCTGT CCACGCAGAC CTCTGTCTCC GAACTCATGG ACGATGAAGC TGAGGACGAT 
GTCAATCTCG CCCAGCTGAG CAGTATCATC CGGCATACGG CGGTCAAGAA GGAACTGGAG 
GCCCAGCAGC ACCAGCAACA AGTACAGCAT GAACAGATGC AGGCCGCCAG GAAGATGGAA 
GAGGATGAAG CCGCTCCCTG TCTGAACCAG CTGCCTGACG TGATTCGCTT GCGAAATATC 
CGCAACAGCC AAGTGCATGC GAGGCCGAAG GACCTGTTTG TACAAGCTCC TGTTGATGAA 
GAATTCAATG CAGACTACAT GGGTGGACTG TCGCCCACTT CCAGCCAGCG GCTAGACATC 
TGCAATGAGC TGCTTTCGGA GATCCGGCGC GATTGGCTAC ACTTCCGGCC CAAAACTCCC 
ACAGATGAGC TGTCCGATAG CCAAGATTGC GAAGTGGGTA AGACGTGTGA TAGTCACGCT 
GTCGACTGGA CGCAGGACAC GCCCATAAGT GTGGAACTCA ATCGCTTAGG AAAGCGAGAA 
GCAGAGGAAA GCGATTCTAG CTCGTACTTT TTGAGCAGTG GCTTTAAATA CAGGGATCTC 
GAGTCAGATG CCCAATTGCT GCAAACGGTC CAAGCTCAGG ACTACGCTCG AGGCAGCAGC 
AAGAGTCCAA ACTGCTCTGG GTCAGGCTCA GGATCTGCGC TAGAGTTTAA TCTCAGTGGT 
GGTGACTCCC TAAACGATAT CAACAACTTG TTGGGCTGTG ACGACGAGGA CGACAACCAC 
GAGCATATGC TAGACAACAT CCTGCAGGAG TGCGCCATGG ATGATCCCAA GGCATTGAAC 
CAAGCTACTA ATTTTTGGAA CGGTATCCTA GATGGCGAGG CAGCCGTGGA CGAAGTGGAA 
ATGGCCGATC AGCTGTTGGA TTGTATAGAC GAGAAGAAAT CTAAGGTGGG TGCAGCCGAT 
ACATCGAAGC GCGCGGGTCG CAACCGCAAG GCAAGATTGA TCCAAGCTCC AGTGGGTAGC 
TCCGCATTTA CTGTGTGTCC CACGGCGGAG ACTCTGAAGG AACGTGAACT TTTCTTTAGT 
CAAGCTCCGC CTGTTGAAGT CCCGCCCAAA GAGAAAACGC CAGCAGAAGT TGAACTGCCG 
GAAGTTGTGG TCAAGGAAAC AGCGAATTTG CCTGTGAAGG TTGAGGCGGT TGAGAAACCC 
CAGTCGGAGG TTCTTCCCGT TGCCCCACAG CCGCAACAGC TTCAGCCGCA GGCTATTACT 
TTGCCCACGA CGCAGCTGAT CAACATACCC GCCCAGATAA GCACGCAAAA GCAGCATCAG 
CAGCCACAGG TGACGCAATT GCTCAATCAG TTTGCCAACG CTGGTCCGCA GATCATCGCC 
CGCACAGAAT ACGGCGGAGG AGCAGCTGTG GGCGATATAG TGACCATAAC CCCGCACACG 
GGGAGCAGTC CACTCCCCAC ACTCACCGCC CAGCAACAAC TGCAGCACCA ATTGGCACAG 
GCTCAACTCC GAAATGTGAC CGTCCAGCAG CGACAGGCGA CACCTCAAAA CCCCAATCAA 
AATCAGAATC CCCAGCAGCA GTTACTCTTC CAAATGCAGC GGGATCCCTC GCGCTCCCTG 
ACGCCCCTAC AAGCTGCCGG CGCTGGCGCC ACTGGCAACC ATTTGATCTA CACAACAAGC 
TGCGGCGAGA TTGTCACAGC AGGAGCTGCA GCTTCTGCGT CTCAGAAGGT CACATATGCC 
ATCCAGAAAG CCGGTGTCTC AAGTGGAGGA GCAACCACCG CCTCAATTGG ACCCAACACA 
GTAATCACGC TGTCCAATGT AAAGGTGCAG AACGATGATA TCTCCTCTGG TGGTAGTGGC 
AACTCAGGAT CCAGTGTGAA CATCATCAAC ACGGCCAGTG GCCAGCAGTT AGCCGTGCAC 
AGCATTGCCG GTATGCAACA GCAGGGCCAG CAACAGGTGC AACAGGTGAC CACCAGTACG 
CCTTTGCTTG TCGCCACACC CACTCGCAAC AATGTGCAGC AGCAACAGCA GCAGTTGGTG 
CAGCAGCAAC CGATTGTTTG GCGGCAGGTG AGCGGAACGA ACACGGCGGT GGCCACCACA 
GCAGTGCTCT CCACCACGGT GGACTCCTCG CCGAAGGCGG TAACCAACCA GATCCTCTGG 
ACCAGCCGGG CCACGCAAAA AAGGCAGCTC AACGGGCCAG AGAACACAGA CATCAACAAA 
CTTCTTCTCA ATCGGAAGTT GCCGCGCAAG CAGCAAATAC AACAACAGCA CCAACAGATT 
CAACTGACAA AGCAGCAGCT TCAACAATTG GTGCAGCATG AGGAGTTGGA TGACTTGGTG 
GCCACATCGA CGGGCAACAG TGGCGATCAG ATGGAAACCG GCGACGGCCT GCAACAACAA 
CAGCAACAGC AGCAACAACA GCAGCAGCTG CAGAAGCTCA GCAAGGTGAT CAAGATGTCG 
CAGTTTCCAC TGCTCGTGTC GGATGTAAGT CTGCAGCAGC AGCAGTCGGT GTCTGGCCAA 
GCTCAGCAGC AACAATTAAA GCCCAACTCT GCGGCGGCGA TTGCAGCTAA TCACAACTAC 
AGCCTGCACC CTGCCTCAAC GGCGATCATC ACCGCCCAGG CGACACCCCA GCAGGCCAAC 
AAGATCTTCT TGACCAGCAG CAACAGTGGT GGCGGCGGAT CCGCTGGTAA CTTTACCATC 
GCCACTGCCA GCGAACTGCA GGCGGCGGCA GCCGGCCAAA AGCTTCACTA CAAGGAGCTG 
CCCAACTCCA ACCTCAAGCT GAACTTTGTG AGCAGTGCAG GGACGGGTGA GCAAACGCAG 
CAGGTGGGCG CCACAACAGT GCTGCTGAAC AAATCCCAAC AGCAGCAGCA ACAACAGCAG 
CATCAAAAGC TGAAAACGTT GGCCACTACA GGAAATTCTC AAACTGGCCA GGGCGATAAG 
CGAGTGCTGT ACACCAGTCT GCTGAATGTG AAACCGCTGC AGAAGAACAA CAGTGGTCAG 
ATGCAGATGC AACTGGGACC GGGAGGATTG CAACAGGTGC TCCGTGGACG ACTCAACAAT 
TCAGCGATCA TTACTAGAAC GCTGCCGCTG GGCACAACTG TTAACAGTAC CGGCGGTGCT 
GTAGGTCCTA CCACCACGAC TATTAGGCAG TTGGTTACCA CGAATGCGAA CAACGTGAGC 
AGCCAAGATG GCAACCTACT TAGTGCAAAG GAAGTGGGGA AAGCCCTGAC AGTGGAGCAG 
GTGATAGCCA GCGCTAACAA CGGAGGAGTT GTGCTGCCCA CGAGCAACAA CAATAATAAC 
AACAATGGCA GTGGAGCTGG CGGAAATGGA GGACCTTTGA ACAGCGGTGT CAATGCCACA 
GGAGCTGGCG GAGGAGGATC TACAGGCGCG GGTGTTGGGG GAGCTGGTGT GACGTCAACC 
ATCAACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(Pc) ( NM_001273968.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260897.1 Drosophila melanogaster enhancer of polycomb, transcript variant E (E(Pc)), mRNA.

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CloneID	OFa12167
Clone ID Related Accession (Same CDS sequence)	NM_001273968.1
Accession Version	NM_001273968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of polycomb, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GACCCGCGTA TAATCCATTG TATACGAATC AATACTCACA AGGGGCGGCG 
GCGGTGGGAA CGGCTGGTGC CATTCTAGCC GCCCTGCCCA CGGGTCTGCT GCCGGGCGGT 
GCTGGAGCGG CAGGCAATGC AAATGTCTTC GGCTCAGCGT CCCAGTACCT GAACTCGTCG 
AATCTGACAA TGGATGCCAT CAGTGGCAGT GGGAATGGAA ACAGCAGTCG CAAGGAGAAG 
CGTCCATACA AGAAGCGCAA GCACAAATTG CCCCGCGACA AGCAGCAGCA CCAGCAGCAG 
CAAACGCAGC AGCACCAGCA ACAACAGTAT TTGCCGGCGT CTGGTGGCGC CGGAGTCTCG 
CCCGCGCACC ACCTGCCCCA CCATCTGCAC CACCTCAGCA GGCAGCAGAG TGCCTCGCCG 
GCAGCCAACG ACTCGATTGC GGACAGCGAG GAGGAGGACT ACTTTGGCGC CGGTGCCCAG 
AATGGAAACA AACTGGGCTC CGAGAGCGAG GAGGAGACGC CGTTTGCATT TAGGCGCAGA 
TCAAGCTGTG TTTACCTACC GACTCGACAA CGAGATGGCC GCTATCCGTG GGACTCAGCC 
GACGAGGATA TGGCGCCCAG TGGTGCTTGT TCGGATGCTA AATACCGTTA CACACTGACC 
TCCCTCAATT ATCCCAGACC ACGCTGCATT GGCTTCGCAC GTCGTCGATT GGGTCGTGGC 
GGTCGCATCC TGCTGGACCG GGCCACAACG AATTTCGACG ACTTTTGGTC GCAGCTTGAC 
TACACGGTGA TGGAAAGCGT GACGGTCAAC AAATGTAGGG ATAAAATCAA GCAACGGGAT 
CCCGATCAGC TGTGCAAGCC CAGCTCACCG CCCACGGTGG TAGATCTGGT TCCCGCTGGA 
AAGCCCGTCG ATGGTGAGCA TCCTGTTCCT GAACTGATAA AGGAGGAGCC GTCAAGCGAT 
ACAGAAAAGC CTGTTGCTGC GACGGAATCT GGGCGGGAGA GCAAAGAACG GGAGCTTGAG 
GAGAAATTTG AGAACGATGT AGATGATGAA TTCGTAGCCA GTGACGATGA GAATGTGTCA 
CGGCTTGGAA TCTACAGTTC AGCGGTTACG TTACCCCAAA GTTGTGCTGA TCTTCGCCAG 
AAACGTCGCC GCCTGCGTCG CAAGAAGCAA CAGTTGCGAG AACTTAATGC CGCAAAGCGT 
CTAAAGAGGT CTTCGGAAGC GGTGGCAACC GCTGAAGATG TTCAACTGGA GGACGAGGAT 
ACTAAACCAG ATCTGCGTCC ATTACCCAGG GCTTACCTTC AGCGTTTGGC ATTGATTCTG 
GGACAAAAAC TAAAGGTGGA AGAGGATGAT AACACGGAAG TTTCCGAGAA TGTGGCGCCA 
GAGTTGCTGG AAATGAATGA TCCGGAGCAG CTGCAGCCCC CGCGAGCCAA TCATCAGCAA 
CACCACCGTT CCAACCACAA CAACAACAAC AATACCGTGT TGAACAACAA CAATAGTAGT 
AGTAGTAACG TTATGAATAA GGATGTTAGC ATTAGCGAAA AAATCAATGT GATAAAGCAG 
GAGGCGGAGG AGCTCGTCGG CGGCAAATGC GAGGATCAAC CGGTAGCTTC CACCTCGGCG 
GCAGCGGCCG CCGCTCGTGC CGCTGAAGCA GCCGCTGCAG CGGAGGCAGC CTCATCGACA 
ACCACTCTTG CGGCACCGAA TCCGGGTCCA TCGCTGGAGG AGGTGGCGAA GACGATAAAG 
CGCGAGATGA TCGATGCCGA CGACTCCAAC GAGCCCCTGA GCAGCATTCG TACAGCAGCT 
GTCATGCTGT CCACGCAGAC CTCTGTCTCC GAACTCATGG ACGATGAAGC TGAGGACGAT 
GTCAATCTCG CCCAGCTGAG CAGTATCATC CGGCATACGG CGGTCAAGAA GGAACTGGAG 
GCCCAGCAGC ACCAGCAACA AGTACAGCAT GAACAGATGC AGGCCGCCAG GAAGATGGAA 
GAGGATGAAG CCGCTCCCTG TCTGAACCAG CTGCCTGACG TGATTCGCTT GCGAAATATC 
CGCAACAGCC AAGTGCATGC GAGGCCGAAG GACCTGTTTG TACAAGCTCC TGTTGATGAA 
GAATTCAATG CAGACTACAT GGGTGGACTG TCGCCCACTT CCAGCCAGCG GCTAGACATC 
TGCAATGAGC TGCTTTCGGA GATCCGGCGC GATTGGCTAC ACTTCCGGCC CAAAACTCCC 
ACAGATGAGC TGTCCGATAG CCAAGATTGC GAAGTGGGTA AGACGTGTGA TAGTCACGCT 
GTCGACTGGA CGCAGGACAC GCCCATAAGT GTGGAACTCA ATCGCTTAGG AAAGCGAGAA 
GCAGAGGAAA GCGATTCTAG CTCGTACTTT TTGAGCAGTG GCTTTAAATA CAGGGATCTC 
GAGTCAGATG CCCAATTGCT GCAAACGGTC CAAGCTCAGG ACTACGCTCG AGGCAGCAGC 
AAGAGTCCAA ACTGCTCTGG GTCAGGCTCA GGATCTGCGC TAGAGTTTAA TCTCAGTGGT 
GGTGACTCCC TAAACGATAT CAACAACTTG TTGGGCTGTG ACGACGAGGA CGACAACCAC 
GAGCATATGC TAGACAACAT CCTGCAGGAG TGCGCCATGG ATGATCCCAA GGCATTGAAC 
CAAGCTACTA ATTTTTGGAA CGGTATCCTA GATGGCGAGG CAGCCGTGGA CGAAGTGGAA 
ATGGCCGATC AGCTGTTGGA TTGTATAGAC GAGAAGAAAT CTAAGGTGGG TGCAGCCGAT 
ACATCGAAGC GCGCGGGTCG CAACCGCAAG GCAAGATTGA TCCAAGCTCC AGTGGGTAGC 
TCCGCATTTA CTGTGTGTCC CACGGCGGAG ACTCTGAAGG AACGTGAACT TTTCTTTAGT 
CAAGCTCCGC CTGTTGAAGT CCCGCCCAAA GAGAAAACGC CAGCAGAAGT TGAACTGCCG 
GAAGTTGTGG TCAAGGAAAC AGCGAATTTG CCTGTGAAGG TTGAGGCGGT TGAGAAACCC 
CAGTCGGAGG TTCTTCCCGT TGCCCCACAG CCGCAACAGC TTCAGCCGCA GGCTATTACT 
TTGCCCACGA CGCAGCTGAT CAACATACCC GCCCAGATAA GCACGCAAAA GCAGCATCAG 
CAGCCACAGG TGACGCAATT GCTCAATCAG TTTGCCAACG CTGGTCCGCA GATCATCGCC 
CGCACAGAAT ACGGCGGAGG AGCAGCTGTG GGCGATATAG TGACCATAAC CCCGCACACG 
GGGAGCAGTC CACTCCCCAC ACTCACCGCC CAGCAACAAC TGCAGCACCA ATTGGCACAG 
GCTCAACTCC GAAATGTGAC CGTCCAGCAG CGACAGGCGA CACCTCAAAA CCCCAATCAA 
AATCAGAATC CCCAGCAGCA GTTACTCTTC CAAATGCAGC GGGATCCCTC GCGCTCCCTG 
ACGCCCCTAC AAGCTGCCGG CGCTGGCGCC ACTGGCAACC ATTTGATCTA CACAACAAGC 
TGCGGCGAGA TTGTCACAGC AGGAGCTGCA GCTTCTGCGT CTCAGAAGGT CACATATGCC 
ATCCAGAAAG CCGGTGTCTC AAGTGGAGGA GCAACCACCG CCTCAATTGG ACCCAACACA 
GTAATCACGC TGTCCAATGT AAAGGTGCAG AACGATGATA TCTCCTCTGG TGGTAGTGGC 
AACTCAGGAT CCAGTGTGAA CATCATCAAC ACGGCCAGTG GCCAGCAGTT AGCCGTGCAC 
AGCATTGCCG GTATGCAACA GCAGGGCCAG CAACAGGTGC AACAGGTGAC CACCAGTACG 
CCTTTGCTTG TCGCCACACC CACTCGCAAC AATGTGCAGC AGCAACAGCA GCAGTTGGTG 
CAGCAGCAAC CGATTGTTTG GCGGCAGGTG AGCGGAACGA ACACGGCGGT GGCCACCACA 
GCAGTGCTCT CCACCACGGT GGACTCCTCG CCGAAGGCGG TAACCAACCA GATCCTCTGG 
ACCAGCCGGG CCACGCAAAA AAGGCAGCTC AACGGGCCAG AGAACACAGA CATCAACAAA 
CTTCTTCTCA ATCGGAAGTT GCCGCGCAAG CAGCAAATAC AACAACAGCA CCAACAGATT 
CAACTGACAA AGCAGCAGCT TCAACAATTG GTGCAGCATG AGGAGTTGGA TGACTTGGTG 
GCCACATCGA CGGGCAACAG TGGCGATCAG ATGGAAACCG GCGACGGCCT GCAACAACAA 
CAGCAACAGC AGCAACAACA GCAGCAGCTG CAGAAGCTCA GCAAGGTGAT CAAGATGTCG 
CAGTTTCCAC TGCTCGTGTC GGATGTAAGT CTGCAGCAGC AGCAGTCGGT GTCTGGCCAA 
GCTCAGCAGC AACAATTAAA GCCCAACTCT GCGGCGGCGA TTGCAGCTAA TCACAACTAC 
AGCCTGCACC CTGCCTCAAC GGCGATCATC ACCGCCCAGG CGACACCCCA GCAGGCCAAC 
AAGATCTTCT TGACCAGCAG CAACAGTGGT GGCGGCGGAT CCGCTGGTAA CTTTACCATC 
GCCACTGCCA GCGAACTGCA GGCGGCGGCA GCCGGCCAAA AGCTTCACTA CAAGGAGCTG 
CCCAACTCCA ACCTCAAGCT GAACTTTGTG AGCAGTGCAG GGACGGGTGA GCAAACGCAG 
CAGGTGGGCG CCACAACAGT GCTGCTGAAC AAATCCCAAC AGCAGCAGCA ACAACAGCAG 
CATCAAAAGC TGAAAACGTT GGCCACTACA GGAAATTCTC AAACTGGCCA GGGCGATAAG 
CGAGTGCTGT ACACCAGTCT GCTGAATGTG AAACCGCTGC AGAAGAACAA CAGTGGTCAG 
ATGCAGATGC AACTGGGACC GGGAGGATTG CAACAGGTGC TCCGTGGACG ACTCAACAAT 
TCAGCGATCA TTACTAGAAC GCTGCCGCTG GGCACAACTG TTAACAGTAC CGGCGGTGCT 
GTAGGTCCTA CCACCACGAC TATTAGGCAG TTGGTTACCA CGAATGCGAA CAACGTGAGC 
AGCCAAGATG GCAACCTACT TAGTGCAAAG GAAGTGGGGA AAGCCCTGAC AGTGGAGCAG 
GTGATAGCCA GCGCTAACAA CGGAGGAGTT GTGCTGCCCA CGAGCAACAA CAATAATAAC 
AACAATGGCA GTGGAGCTGG CGGAAATGGA GGACCTTTGA ACAGCGGTGT CAATGCCACA 
GGAGCTGGCG GAGGAGGATC TACAGGCGCG GGTGTTGGGG GAGCTGGTGT GACGTCAACC 
ATCAACAGAT GAGACGCGGA TGGCAGCGGC AGCAACAGCT AACATCGAGC ACAACCCTTG 
CAAGTGCCCA ACGGCCCTCT GCTCAGTTTG GCATCAACGC AAACGGAGGC GGAAACCCAG 
GCGGTGGCCA TATCCATGCC TTTGGCGGAC GCCCACGTGG CCTTGGGCCA GCTCCTGCAG 
CAGCATCACT GCAGCTGCAC CGTTTGTTTG GTGAACAAGA GCGAGCTGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260897.1
CDS	1266..7559
Translation	MSKLSFRARHLDPSKQMPIYLAEELPDLPEYSAINRAVPQMPSGMEKEEESEHHLQRAICTGLIIPTPEVLQTDQPFYDAYYPPDYKMPRQMIHMQPLGLDTEVPDYDMDSADEDWLSQQQRLELTELKFEQMMDRLEKSSGQTVVTLNEAKSLLNQDDETSISVYDYWLNKRLKMQHPLILTVKTESRPGASSNNPYLAFRRRTEKMQTRKNRKNDEASYEKMLKLRRDLQRATTILEMVRRREETKRDHLKMTVNIFEKRVEMRDFNGAVYSELNSQYKNTRPAYNPLYTNQYSQGAAAVGTAGAILAALPTGLLPGGAGAAGNANVFGSASQYLNSSNLTMDAISGSGNGNSSRKEKRPYKKRKHKLPRDKQQHQQQQTQQHQQQQYLPASGGAGVSPAHHLPHHLHHLSRQQSASPAANDSIADSEEEDYFGAGAQNGNKLGSESEEETPFAFRRRSSCVYLPTRQRDGRYPWDSADEDMAPSGACSDAKYRYTLTSLNYPRPRCIGFARRRLGRGGRILLDRATTNFDDFWSQLDYTVMESVTVNKCRDKIKQRDPDQLCKPSSPPTVVDLVPAGKPVDGEHPVPELIKEEPSSDTEKPVAATESGRESKERELEEKFENDVDDEFVASDDENVSRLGIYSSAVTLPQSCADLRQKRRRLRRKKQQLRELNAAKRLKRSSEAVATAEDVQLEDEDTKPDLRPLPRAYLQRLALILGQKLKVEEDDNTEVSENVAPELLEMNDPEQLQPPRANHQQHHRSNHNNNNNTVLNNNNSSSSNVMNKDVSISEKINVIKQEAEELVGGKCEDQPVASTSAAAAAARAAEAAAAAEAASSTTTLAAPNPGPSLEEVAKTIKREMIDADDSNEPLSSIRTAAVMLSTQTSVSELMDDEAEDDVNLAQLSSIIRHTAVKKELEAQQHQQQVQHEQMQAARKMEEDEAAPCLNQLPDVIRLRNIRNSQVHARPKDLFVQAPVDEEFNADYMGGLSPTSSQRLDICNELLSEIRRDWLHFRPKTPTDELSDSQDCEVGKTCDSHAVDWTQDTPISVELNRLGKREAEESDSSSYFLSSGFKYRDLESDAQLLQTVQAQDYARGSSKSPNCSGSGSGSALEFNLSGGDSLNDINNLLGCDDEDDNHEHMLDNILQECAMDDPKALNQATNFWNGILDGEAAVDEVEMADQLLDCIDEKKSKVGAADTSKRAGRNRKARLIQAPVGSSAFTVCPTAETLKERELFFSQAPPVEVPPKEKTPAEVELPEVVVKETANLPVKVEAVEKPQSEVLPVAPQPQQLQPQAITLPTTQLINIPAQISTQKQHQQPQVTQLLNQFANAGPQIIARTEYGGGAAVGDIVTITPHTGSSPLPTLTAQQQLQHQLAQAQLRNVTVQQRQATPQNPNQNQNPQQQLLFQMQRDPSRSLTPLQAAGAGATGNHLIYTTSCGEIVTAGAAASASQKVTYAIQKAGVSSGGATTASIGPNTVITLSNVKVQNDDISSGGSGNSGSSVNIINTASGQQLAVHSIAGMQQQGQQQVQQVTTSTPLLVATPTRNNVQQQQQQLVQQQPIVWRQVSGTNTAVATTAVLSTTVDSSPKAVTNQILWTSRATQKRQLNGPENTDINKLLLNRKLPRKQQIQQQHQQIQLTKQQLQQLVQHEELDDLVATSTGNSGDQMETGDGLQQQQQQQQQQQQLQKLSKVIKMSQFPLLVSDVSLQQQQSVSGQAQQQQLKPNSAAAIAANHNYSLHPASTAIITAQATPQQANKIFLTSSNSGGGGSAGNFTIATASELQAAAAGQKLHYKELPNSNLKLNFVSSAGTGEQTQQVGATTVLLNKSQQQQQQQQHQKLKTLATTGNSQTGQGDKRVLYTSLLNVKPLQKNNSGQMQMQLGPGGLQQVLRGRLNNSAIITRTLPLGTTVNSTGGAVGPTTTTIRQLVTTNANNVSSQDGNLLSAKEVGKALTVEQVIASANNGGVVLPTSNNNNNNNGSGAGGNGGPLNSGVNATGAGGGGSTGAGVGGAGVTSTINRDADGSGSNSHRAQPLQVPNGPLLSLASTQTEAETQAVAISMPLADAHVALGQLLQQHHCSCTVCLVNKSELV

Target ORF information:

RefSeq Version	NM_001273968.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of polycomb, transcript variant E (E(Pc)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273968.1

ORF Insert Sequence:

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GACCCGCGTA TAATCCATTG TATACGAATC AATACTCACA AGGGGCGGCG 
GCGGTGGGAA CGGCTGGTGC CATTCTAGCC GCCCTGCCCA CGGGTCTGCT GCCGGGCGGT 
GCTGGAGCGG CAGGCAATGC AAATGTCTTC GGCTCAGCGT CCCAGTACCT GAACTCGTCG 
AATCTGACAA TGGATGCCAT CAGTGGCAGT GGGAATGGAA ACAGCAGTCG CAAGGAGAAG 
CGTCCATACA AGAAGCGCAA GCACAAATTG CCCCGCGACA AGCAGCAGCA CCAGCAGCAG 
CAAACGCAGC AGCACCAGCA ACAACAGTAT TTGCCGGCGT CTGGTGGCGC CGGAGTCTCG 
CCCGCGCACC ACCTGCCCCA CCATCTGCAC CACCTCAGCA GGCAGCAGAG TGCCTCGCCG 
GCAGCCAACG ACTCGATTGC GGACAGCGAG GAGGAGGACT ACTTTGGCGC CGGTGCCCAG 
AATGGAAACA AACTGGGCTC CGAGAGCGAG GAGGAGACGC CGTTTGCATT TAGGCGCAGA 
TCAAGCTGTG TTTACCTACC GACTCGACAA CGAGATGGCC GCTATCCGTG GGACTCAGCC 
GACGAGGATA TGGCGCCCAG TGGTGCTTGT TCGGATGCTA AATACCGTTA CACACTGACC 
TCCCTCAATT ATCCCAGACC ACGCTGCATT GGCTTCGCAC GTCGTCGATT GGGTCGTGGC 
GGTCGCATCC TGCTGGACCG GGCCACAACG AATTTCGACG ACTTTTGGTC GCAGCTTGAC 
TACACGGTGA TGGAAAGCGT GACGGTCAAC AAATGTAGGG ATAAAATCAA GCAACGGGAT 
CCCGATCAGC TGTGCAAGCC CAGCTCACCG CCCACGGTGG TAGATCTGGT TCCCGCTGGA 
AAGCCCGTCG ATGGTGAGCA TCCTGTTCCT GAACTGATAA AGGAGGAGCC GTCAAGCGAT 
ACAGAAAAGC CTGTTGCTGC GACGGAATCT GGGCGGGAGA GCAAAGAACG GGAGCTTGAG 
GAGAAATTTG AGAACGATGT AGATGATGAA TTCGTAGCCA GTGACGATGA GAATGTGTCA 
CGGCTTGGAA TCTACAGTTC AGCGGTTACG TTACCCCAAA GTTGTGCTGA TCTTCGCCAG 
AAACGTCGCC GCCTGCGTCG CAAGAAGCAA CAGTTGCGAG AACTTAATGC CGCAAAGCGT 
CTAAAGAGGT CTTCGGAAGC GGTGGCAACC GCTGAAGATG TTCAACTGGA GGACGAGGAT 
ACTAAACCAG ATCTGCGTCC ATTACCCAGG GCTTACCTTC AGCGTTTGGC ATTGATTCTG 
GGACAAAAAC TAAAGGTGGA AGAGGATGAT AACACGGAAG TTTCCGAGAA TGTGGCGCCA 
GAGTTGCTGG AAATGAATGA TCCGGAGCAG CTGCAGCCCC CGCGAGCCAA TCATCAGCAA 
CACCACCGTT CCAACCACAA CAACAACAAC AATACCGTGT TGAACAACAA CAATAGTAGT 
AGTAGTAACG TTATGAATAA GGATGTTAGC ATTAGCGAAA AAATCAATGT GATAAAGCAG 
GAGGCGGAGG AGCTCGTCGG CGGCAAATGC GAGGATCAAC CGGTAGCTTC CACCTCGGCG 
GCAGCGGCCG CCGCTCGTGC CGCTGAAGCA GCCGCTGCAG CGGAGGCAGC CTCATCGACA 
ACCACTCTTG CGGCACCGAA TCCGGGTCCA TCGCTGGAGG AGGTGGCGAA GACGATAAAG 
CGCGAGATGA TCGATGCCGA CGACTCCAAC GAGCCCCTGA GCAGCATTCG TACAGCAGCT 
GTCATGCTGT CCACGCAGAC CTCTGTCTCC GAACTCATGG ACGATGAAGC TGAGGACGAT 
GTCAATCTCG CCCAGCTGAG CAGTATCATC CGGCATACGG CGGTCAAGAA GGAACTGGAG 
GCCCAGCAGC ACCAGCAACA AGTACAGCAT GAACAGATGC AGGCCGCCAG GAAGATGGAA 
GAGGATGAAG CCGCTCCCTG TCTGAACCAG CTGCCTGACG TGATTCGCTT GCGAAATATC 
CGCAACAGCC AAGTGCATGC GAGGCCGAAG GACCTGTTTG TACAAGCTCC TGTTGATGAA 
GAATTCAATG CAGACTACAT GGGTGGACTG TCGCCCACTT CCAGCCAGCG GCTAGACATC 
TGCAATGAGC TGCTTTCGGA GATCCGGCGC GATTGGCTAC ACTTCCGGCC CAAAACTCCC 
ACAGATGAGC TGTCCGATAG CCAAGATTGC GAAGTGGGTA AGACGTGTGA TAGTCACGCT 
GTCGACTGGA CGCAGGACAC GCCCATAAGT GTGGAACTCA ATCGCTTAGG AAAGCGAGAA 
GCAGAGGAAA GCGATTCTAG CTCGTACTTT TTGAGCAGTG GCTTTAAATA CAGGGATCTC 
GAGTCAGATG CCCAATTGCT GCAAACGGTC CAAGCTCAGG ACTACGCTCG AGGCAGCAGC 
AAGAGTCCAA ACTGCTCTGG GTCAGGCTCA GGATCTGCGC TAGAGTTTAA TCTCAGTGGT 
GGTGACTCCC TAAACGATAT CAACAACTTG TTGGGCTGTG ACGACGAGGA CGACAACCAC 
GAGCATATGC TAGACAACAT CCTGCAGGAG TGCGCCATGG ATGATCCCAA GGCATTGAAC 
CAAGCTACTA ATTTTTGGAA CGGTATCCTA GATGGCGAGG CAGCCGTGGA CGAAGTGGAA 
ATGGCCGATC AGCTGTTGGA TTGTATAGAC GAGAAGAAAT CTAAGGTGGG TGCAGCCGAT 
ACATCGAAGC GCGCGGGTCG CAACCGCAAG GCAAGATTGA TCCAAGCTCC AGTGGGTAGC 
TCCGCATTTA CTGTGTGTCC CACGGCGGAG ACTCTGAAGG AACGTGAACT TTTCTTTAGT 
CAAGCTCCGC CTGTTGAAGT CCCGCCCAAA GAGAAAACGC CAGCAGAAGT TGAACTGCCG 
GAAGTTGTGG TCAAGGAAAC AGCGAATTTG CCTGTGAAGG TTGAGGCGGT TGAGAAACCC 
CAGTCGGAGG TTCTTCCCGT TGCCCCACAG CCGCAACAGC TTCAGCCGCA GGCTATTACT 
TTGCCCACGA CGCAGCTGAT CAACATACCC GCCCAGATAA GCACGCAAAA GCAGCATCAG 
CAGCCACAGG TGACGCAATT GCTCAATCAG TTTGCCAACG CTGGTCCGCA GATCATCGCC 
CGCACAGAAT ACGGCGGAGG AGCAGCTGTG GGCGATATAG TGACCATAAC CCCGCACACG 
GGGAGCAGTC CACTCCCCAC ACTCACCGCC CAGCAACAAC TGCAGCACCA ATTGGCACAG 
GCTCAACTCC GAAATGTGAC CGTCCAGCAG CGACAGGCGA CACCTCAAAA CCCCAATCAA 
AATCAGAATC CCCAGCAGCA GTTACTCTTC CAAATGCAGC GGGATCCCTC GCGCTCCCTG 
ACGCCCCTAC AAGCTGCCGG CGCTGGCGCC ACTGGCAACC ATTTGATCTA CACAACAAGC 
TGCGGCGAGA TTGTCACAGC AGGAGCTGCA GCTTCTGCGT CTCAGAAGGT CACATATGCC 
ATCCAGAAAG CCGGTGTCTC AAGTGGAGGA GCAACCACCG CCTCAATTGG ACCCAACACA 
GTAATCACGC TGTCCAATGT AAAGGTGCAG AACGATGATA TCTCCTCTGG TGGTAGTGGC 
AACTCAGGAT CCAGTGTGAA CATCATCAAC ACGGCCAGTG GCCAGCAGTT AGCCGTGCAC 
AGCATTGCCG GTATGCAACA GCAGGGCCAG CAACAGGTGC AACAGGTGAC CACCAGTACG 
CCTTTGCTTG TCGCCACACC CACTCGCAAC AATGTGCAGC AGCAACAGCA GCAGTTGGTG 
CAGCAGCAAC CGATTGTTTG GCGGCAGGTG AGCGGAACGA ACACGGCGGT GGCCACCACA 
GCAGTGCTCT CCACCACGGT GGACTCCTCG CCGAAGGCGG TAACCAACCA GATCCTCTGG 
ACCAGCCGGG CCACGCAAAA AAGGCAGCTC AACGGGCCAG AGAACACAGA CATCAACAAA 
CTTCTTCTCA ATCGGAAGTT GCCGCGCAAG CAGCAAATAC AACAACAGCA CCAACAGATT 
CAACTGACAA AGCAGCAGCT TCAACAATTG GTGCAGCATG AGGAGTTGGA TGACTTGGTG 
GCCACATCGA CGGGCAACAG TGGCGATCAG ATGGAAACCG GCGACGGCCT GCAACAACAA 
CAGCAACAGC AGCAACAACA GCAGCAGCTG CAGAAGCTCA GCAAGGTGAT CAAGATGTCG 
CAGTTTCCAC TGCTCGTGTC GGATGTAAGT CTGCAGCAGC AGCAGTCGGT GTCTGGCCAA 
GCTCAGCAGC AACAATTAAA GCCCAACTCT GCGGCGGCGA TTGCAGCTAA TCACAACTAC 
AGCCTGCACC CTGCCTCAAC GGCGATCATC ACCGCCCAGG CGACACCCCA GCAGGCCAAC 
AAGATCTTCT TGACCAGCAG CAACAGTGGT GGCGGCGGAT CCGCTGGTAA CTTTACCATC 
GCCACTGCCA GCGAACTGCA GGCGGCGGCA GCCGGCCAAA AGCTTCACTA CAAGGAGCTG 
CCCAACTCCA ACCTCAAGCT GAACTTTGTG AGCAGTGCAG GGACGGGTGA GCAAACGCAG 
CAGGTGGGCG CCACAACAGT GCTGCTGAAC AAATCCCAAC AGCAGCAGCA ACAACAGCAG 
CATCAAAAGC TGAAAACGTT GGCCACTACA GGAAATTCTC AAACTGGCCA GGGCGATAAG 
CGAGTGCTGT ACACCAGTCT GCTGAATGTG AAACCGCTGC AGAAGAACAA CAGTGGTCAG 
ATGCAGATGC AACTGGGACC GGGAGGATTG CAACAGGTGC TCCGTGGACG ACTCAACAAT 
TCAGCGATCA TTACTAGAAC GCTGCCGCTG GGCACAACTG TTAACAGTAC CGGCGGTGCT 
GTAGGTCCTA CCACCACGAC TATTAGGCAG TTGGTTACCA CGAATGCGAA CAACGTGAGC 
AGCCAAGATG GCAACCTACT TAGTGCAAAG GAAGTGGGGA AAGCCCTGAC AGTGGAGCAG 
GTGATAGCCA GCGCTAACAA CGGAGGAGTT GTGCTGCCCA CGAGCAACAA CAATAATAAC 
AACAATGGCA GTGGAGCTGG CGGAAATGGA GGACCTTTGA ACAGCGGTGT CAATGCCACA 
GGAGCTGGCG GAGGAGGATC TACAGGCGCG GGTGTTGGGG GAGCTGGTGT GACGTCAACC 
ATCAACAGAT GAGACGCGGA TGGCAGCGGC AGCAACAGCT AACATCGAGC ACAACCCTTG 
CAAGTGCCCA ACGGCCCTCT GCTCAGTTTG GCATCAACGC AAACGGAGGC GGAAACCCAG 
GCGGTGGCCA TATCCATGCC TTTGGCGGAC GCCCACGTGG CCTTGGGCCA GCTCCTGCAG 
CAGCATCACT GCAGCTGCAC CGTTTGTTTG GTGAACAAGA GCGAGCTGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(Pc) ( NM_001273967.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260896.1 Drosophila melanogaster enhancer of polycomb, transcript variant D (E(Pc)), mRNA.

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CloneID	OFa12168
Clone ID Related Accession (Same CDS sequence)	NM_001273967.1
Accession Version	NM_001273967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of polycomb, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GACCCGCGTA TAATCCATTG TATACGAATC AATACTCACA AGGGGCGGCG 
GCGGTGGGAA CGGCTGGTGC CATTCTAGCC GCCCTGCCCA CGGGTCTGCT GCCGGGCGGT 
GCTGGAGCGG CAGGCAATGC AAATGTCTTC GGCTCAGCGT CCCAGTACCT GAACTCGTCG 
AATCTGACAA TGGATGCCAT CAGTGGCAGT GGGAATGGAA ACAGCAGTCG CAAGGAGAAG 
CGTCCATACA AGAAGCGCAA GCACAAATTG CCCCGCGACA AGCAGCAGCA CCAGCAGCAG 
CAAACGCAGC AGCACCAGCA ACAACAGTAT TTGCCGGCGT CTGGTGGCGC CGGAGTCTCG 
CCCGCGCACC ACCTGCCCCA CCATCTGCAC CACCTCAGCA GGCAGCAGAG TGCCTCGCCG 
GCAGCCAACG ACTCGATTGC GGACAGCGAG GAGGAGGACT ACTTTGGCGC CGGTGCCCAG 
AATGGAAACA AACTGGGCTC CGAGAGCGAG GAGGAGACGC CGTTTGCATT TAGGCGCAGA 
TCAAGCTGTG TTTACCTACC GACTCGACAA CGAGATGGCC GCTATCCGTG GGACTCAGCC 
GACGAGGATA TGGCGCCCAG TGGTGCTTGT TCGGATGCTA AATACCGTTA CACACTGACC 
TCCCTCAATT ATCCCAGACC ACGCTGCATT GGCTTCGCAC GTCGTCGATT GGGTCGTGGC 
GGTCGCATCC TGCTGGACCG GGCCACAACG AATTTCGACG ACTTTTGGTC GCAGCTTGAC 
TACACGGTGA TGGAAAGCGT GACGGTCAAC AAATGTAGGG ATAAAATCAA GCAACGGGAT 
CCCGATCAGC TGTGCAAGCC CAGCTCACCG CCCACGGTGG TAGATCTGGT TCCCGCTGGA 
AAGCCCGTCG ATGGTGAGCA TCCTGTTCCT GAACTGATAA AGGAGGAGCC GTCAAGCGAT 
ACAGAAAAGC CTGTTGCTGC GACGGAATCT GGGCGGGAGA GCAAAGAACG GGAGCTTGAG 
GAGAAATTTG AGAACGATGT AGATGATGAA TTCGTAGCCA GTGACGATGA GAATGTGTCA 
CGGCTTGGAA TCTACAGTTC AGCGGTTACG TTACCCCAAA GTTGTGCTGA TCTTCGCCAG 
AAACGTCGCC GCCTGCGTCG CAAGAAGCAA CAGTTGCGAG AACTTAATGC CGCAAAGCGT 
CTAAAGAGGT CTTCGGAAGC GGTGGCAACC GCTGAAGATG TTCAACTGGA GGACGAGGAT 
ACTAAACCAG ATCTGCGTCC ATTACCCAGG GCTTACCTTC AGCGTTTGGC ATTGATTCTG 
GGACAAAAAC TAAAGGTGGA AGAGGATGAT AACACGGAAG TTTCCGAGAA TGTGGCGCCA 
GAGTTGCTGG AAATGAATGA TCCGGAGCAG CTGCAGCCCC CGCGAGCCAA TCATCAGCAA 
CACCACCGTT CCAACCACAA CAACAACAAC AATACCGTGT TGAACAACAA CAATAGTAGT 
AGTAGTAACG TTATGAATAA GGATGTTAGC ATTAGCGAAA AAATCAATGT GATAAAGCAG 
GAGGCGGAGG AGCTCGTCGG CGGCAAATGC GAGGATCAAC CGGTAGCTTC CACCTCGGCG 
GCAGCGGCCG CCGCTCGTGC CGCTGAAGCA GCCGCTGCAG CGGAGGCAGC CTCATCGACA 
ACCACTCTTG CGGCACCGAA TCCGGGTCCA TCGCTGGAGG AGGTGGCGAA GACGATAAAG 
CGCGAGATGA TCGATGCCGA CGACTCCAAC GAGCCCCTGA GCAGCATTCG TACAGCAGCT 
GTCATGCTGT CCACGCAGAC CTCTGTCTCC GAACTCATGG ACGATGAAGC TGAGGACGAT 
GTCAATCTCG CCCAGCTGAG CAGTATCATC CGGCATACGG CGGTCAAGAA GGAACTGGAG 
GCCCAGCAGC ACCAGCAACA AGTACAGCAT GAACAGATGC AGGCCGCCAG GAAGATGGAA 
GAGGATGAAG CCGCTCCCTG TCTGAACCAG CTGCCTGACG TGATTCGCTT GCGAAATATC 
CGCAACAGCC AAGTGCATGC GAGGCCGAAG GACCTGTTTG TACAAGCTCC TGTTGATGAA 
GAATTCAATG CAGACTACAT GGGTGGACTG TCGCCCACTT CCAGCCAGCG GCTAGACATC 
TGCAATGAGC TGCTTTCGGA GATCCGGCGC GATTGGCTAC ACTTCCGGCC CAAAACTCCC 
ACAGATGAGC TGTCCGATAG CCAAGATTGC GAAGTGGGTA AGACGTGTGA TAGTCACGCT 
GTCGACTGGA CGCAGGACAC GCCCATAAGT GTGGAACTCA ATCGCTTAGG AAAGCGAGAA 
GCAGAGGAAA GCGATTCTAG CTCGTACTTT TTGAGCAGTG GCTTTAAATA CAGGGATCTC 
GAGTCAGATG CCCAATTGCT GCAAACGGTC CAAGCTCAGG ACTACGCTCG AGGCAGCAGC 
AAGAGTCCAA ACTGCTCTGG GTCAGGCTCA GGATCTGCGC TAGAGTTTAA TCTCAGTGGT 
GGTGACTCCC TAAACGATAT CAACAACTTG TTGGGCTGTG ACGACGAGGA CGACAACCAC 
GAGCATATGC TAGACAACAT CCTGCAGGAG TGCGCCATGG ATGATCCCAA GGCATTGAAC 
CAAGCTACTA ATTTTTGGAA CGGTATCCTA GATGGCGAGG CAGCCGTGGA CGAAGTGGAA 
ATGGCCGATC AGCTGTTGGA TTGTATAGAC GAGAAGAAAT CTAAGGTGGG TGCAGCCGAT 
ACATCGAAGC GCGCGGGTCG CAACCGCAAG GCAAGATTGA TCCAAGCTCC AGTGGGTAGC 
TCCGCATTTA CTGTGTGTCC CACGGCGGAG ACTCTGAAGG AACGTGAACT TTTCTTTAGT 
CAAGCTCCGC CTGTTGAAGT CCCGCCCAAA GAGAAAACGC CAGCAGAAGT TGAACTGCCG 
GAAGTTGTGG TCAAGGAAAC AGCGAATTTG CCTGTGAAGG TTGAGGCGGT TGAGAAACCC 
CAGTCGGAGG TTCTTCCCGT TGCCCCACAG CCGCAACAGC TTCAGCCGCA GGCTATTACT 
TTGCCCACGA CGCAGCTGAT CAACATACCC GCCCAGATAA GCACGCAAAA GCAGCATCAG 
CAGCCACAGG TGACGCAATT GCTCAATCAG TTTGCCAACG CTGGTCCGCA GATCATCGCC 
CGCACAGAAT ACGGCGGAGG AGCAGCTGTG GGCGATATAG TGACCATAAC CCCGCACACG 
GGGAGCAGTC CACTCCCCAC ACTCACCGCC CAGCAACAAC TGCAGCACCA ATTGGCACAG 
GCTCAACTCC GAAATGTGAC CGTCCAGCAG CGACAGGCGA CACCTCAAAA CCCCAATCAA 
AATCAGAATC CCCAGCAGCA GTTACTCTTC CAAATGCAGC GGGATCCCTC GCGCTCCCTG 
ACGCCCCTAC AAGCTGCCGG CGCTGGCGCC ACTGGCAACC ATTTGATCTA CACAACAAGC 
TGCGGCGAGA TTGTCACAGC AGGAGCTGCA GCTTCTGCGT CTCAGAAGGT CACATATGCC 
ATCCAGAAAG CCGGTGTCTC AAGTGGAGGA GCAACCACCG CCTCAATTGG ACCCAACACA 
GTAATCACGC TGTCCAATGT AAAGGTGCAG AACGATGATA TCTCCTCTGG TGGTAGTGGC 
AACTCAGGAT CCAGTGTGAA CATCATCAAC ACGGCCAGTG GCCAGCAGTT AGCCGTGCAC 
AGCATTGCCG GTATGCAACA GCAGGGCCAG CAACAGGTGC AACAGGTGAC CACCAGTACG 
CCTTTGCTTG TCGCCACACC CACTCGCAAC AATGTGCAGC AGCAACAGCA GCAGTTGGTG 
CAGCAGCAAC CGATTGTTTG GCGGCAGGTG AGCGGAACGA ACACGGCGGT GGCCACCACA 
GCAGTGCTCT CCACCACGGT GGACTCCTCG CCGAAGGCGG TAACCAACCA GATCCTCTGG 
ACCAGCCGGG CCACGCAAAA AAGGCAGCTC AACGGGCCAG AGAACACAGA CATCAACAAA 
CTTCTTCTCA ATCGGAAGTT GCCGCGCAAG CAGCAAATAC AACAACAGCA CCAACAGATT 
CAACTGACAA AGCAGCAGCT TCAACAATTG GTGCAGCATG AGGAGTTGGA TGACTTGGTG 
GCCACATCGA CGGGCAACAG TGGCGATCAG ATGGAAACCG GCGACGGCCT GCAACAACAA 
CAGCAACAGC AGCAACAACA GCAGCAGCTG CAGAAGCTCA GCAAGGTGAT CAAGATGTCG 
CAGTTTCCAC TGCTCGTGTC GGATGTAAGT CTGCAGCAGC AGCAGTCGGT GTCTGGCCAA 
GCTCAGCAGC AACAATTAAA GCCCAACTCT GCGGCGGCGA TTGCAGCTAA TCACAACTAC 
AGCCTGCACC CTGCCTCAAC GGCGATCATC ACCGCCCAGG CGACACCCCA GCAGGCCAAC 
AAGATCTTCT TGACCAGCAG CAACAGTGGT GGCGGCGGAT CCGCTGGTAA CTTTACCATC 
GCCACTGCCA GCGAACTGCA GGCGGCGGCA GCCGGCCAAA AGCTTCACTA CAAGGAGCTG 
CCCAACTCCA ACCTCAAGCT GAACTTTGTG AGCAGTGCAG GGACGGGTGA GCAAACGCAG 
CAGGTGGGCG CCACAACAGT GCTGCTGAAC AAATCCCAAC AGCAGCAGCA ACAACAGCAG 
CATCAAAAGC TGAAAACGTT GGCCACTACA GGAAATTCTC AAACTGGCCA GGGCGATAAG 
CGAGTGCTGT ACACCAGTCT GCTGAATGTG AAACCGCTGC AGAAGAACAA CAGTGGTCAG 
ATGCAGATGC AACTGGGACC GGGAGGATTG CAACAGGTGC TCCGTGGACG ACTCAACAAT 
TCAGCGATCA TTACTAGAAC GCTGCCGCTG GGCACAACTG TTAACAGTAC CGGCGGTGCT 
GTAGGTCCTA CCACCACGAC TATTAGGCAG TTGGTTACCA CGAATGCGAA CAACGTGAGC 
AGCCAAGATG GCAACCTACT TAGTGCAAAG GAAGTGGGGA AAGCCCTGAC AGTGGAGCAG 
GTGATAGCCA GCGCTAACAA CGGAGGAGTT GTGCTGCCCA CGAGCAACAA CAATAATAAC 
AACAATGGCA GTGGAGCTGG CGGAAATGGA GGACCTTTGA ACAGCGGTGT CAATGCCACA 
GGAGCTGGCG GAGGAGGATC TACAGGCGCG GGTGTTGGGG GAGCTGGTGT GACGTCAACC 
ATCAACAGAT GAGACGCGGA TGGCAGCGGC AGCAACAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260896.1
CDS	1266..7367
Translation	MSKLSFRARHLDPSKQMPIYLAEELPDLPEYSAINRAVPQMPSGMEKEEESEHHLQRAICTGLIIPTPEVLQTDQPFYDAYYPPDYKMPRQMIHMQPLGLDTEVPDYDMDSADEDWLSQQQRLELTELKFEQMMDRLEKSSGQTVVTLNEAKSLLNQDDETSISVYDYWLNKRLKMQHPLILTVKTESRPGASSNNPYLAFRRRTEKMQTRKNRKNDEASYEKMLKLRRDLQRATTILEMVRRREETKRDHLKMTVNIFEKRVEMRDFNGAVYSELNSQYKNTRPAYNPLYTNQYSQGAAAVGTAGAILAALPTGLLPGGAGAAGNANVFGSASQYLNSSNLTMDAISGSGNGNSSRKEKRPYKKRKHKLPRDKQQHQQQQTQQHQQQQYLPASGGAGVSPAHHLPHHLHHLSRQQSASPAANDSIADSEEEDYFGAGAQNGNKLGSESEEETPFAFRRRSSCVYLPTRQRDGRYPWDSADEDMAPSGACSDAKYRYTLTSLNYPRPRCIGFARRRLGRGGRILLDRATTNFDDFWSQLDYTVMESVTVNKCRDKIKQRDPDQLCKPSSPPTVVDLVPAGKPVDGEHPVPELIKEEPSSDTEKPVAATESGRESKERELEEKFENDVDDEFVASDDENVSRLGIYSSAVTLPQSCADLRQKRRRLRRKKQQLRELNAAKRLKRSSEAVATAEDVQLEDEDTKPDLRPLPRAYLQRLALILGQKLKVEEDDNTEVSENVAPELLEMNDPEQLQPPRANHQQHHRSNHNNNNNTVLNNNNSSSSNVMNKDVSISEKINVIKQEAEELVGGKCEDQPVASTSAAAAAARAAEAAAAAEAASSTTTLAAPNPGPSLEEVAKTIKREMIDADDSNEPLSSIRTAAVMLSTQTSVSELMDDEAEDDVNLAQLSSIIRHTAVKKELEAQQHQQQVQHEQMQAARKMEEDEAAPCLNQLPDVIRLRNIRNSQVHARPKDLFVQAPVDEEFNADYMGGLSPTSSQRLDICNELLSEIRRDWLHFRPKTPTDELSDSQDCEVGKTCDSHAVDWTQDTPISVELNRLGKREAEESDSSSYFLSSGFKYRDLESDAQLLQTVQAQDYARGSSKSPNCSGSGSGSALEFNLSGGDSLNDINNLLGCDDEDDNHEHMLDNILQECAMDDPKALNQATNFWNGILDGEAAVDEVEMADQLLDCIDEKKSKVGAADTSKRAGRNRKARLIQAPVGSSAFTVCPTAETLKERELFFSQAPPVEVPPKEKTPAEVELPEVVVKETANLPVKVEAVEKPQSEVLPVAPQPQQLQPQAITLPTTQLINIPAQISTQKQHQQPQVTQLLNQFANAGPQIIARTEYGGGAAVGDIVTITPHTGSSPLPTLTAQQQLQHQLAQAQLRNVTVQQRQATPQNPNQNQNPQQQLLFQMQRDPSRSLTPLQAAGAGATGNHLIYTTSCGEIVTAGAAASASQKVTYAIQKAGVSSGGATTASIGPNTVITLSNVKVQNDDISSGGSGNSGSSVNIINTASGQQLAVHSIAGMQQQGQQQVQQVTTSTPLLVATPTRNNVQQQQQQLVQQQPIVWRQVSGTNTAVATTAVLSTTVDSSPKAVTNQILWTSRATQKRQLNGPENTDINKLLLNRKLPRKQQIQQQHQQIQLTKQQLQQLVQHEELDDLVATSTGNSGDQMETGDGLQQQQQQQQQQQQLQKLSKVIKMSQFPLLVSDVSLQQQQSVSGQAQQQQLKPNSAAAIAANHNYSLHPASTAIITAQATPQQANKIFLTSSNSGGGGSAGNFTIATASELQAAAAGQKLHYKELPNSNLKLNFVSSAGTGEQTQQVGATTVLLNKSQQQQQQQQHQKLKTLATTGNSQTGQGDKRVLYTSLLNVKPLQKNNSGQMQMQLGPGGLQQVLRGRLNNSAIITRTLPLGTTVNSTGGAVGPTTTTIRQLVTTNANNVSSQDGNLLSAKEVGKALTVEQVIASANNGGVVLPTSNNNNNNNGSGAGGNGGPLNSGVNATGAGGGGSTGAGVGGAGVTSTINR*DADGSGSNS

Target ORF information:

RefSeq Version	NM_001273967.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of polycomb, transcript variant D (E(Pc)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273967.1

ORF Insert Sequence:

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GACCCGCGTA TAATCCATTG TATACGAATC AATACTCACA AGGGGCGGCG 
GCGGTGGGAA CGGCTGGTGC CATTCTAGCC GCCCTGCCCA CGGGTCTGCT GCCGGGCGGT 
GCTGGAGCGG CAGGCAATGC AAATGTCTTC GGCTCAGCGT CCCAGTACCT GAACTCGTCG 
AATCTGACAA TGGATGCCAT CAGTGGCAGT GGGAATGGAA ACAGCAGTCG CAAGGAGAAG 
CGTCCATACA AGAAGCGCAA GCACAAATTG CCCCGCGACA AGCAGCAGCA CCAGCAGCAG 
CAAACGCAGC AGCACCAGCA ACAACAGTAT TTGCCGGCGT CTGGTGGCGC CGGAGTCTCG 
CCCGCGCACC ACCTGCCCCA CCATCTGCAC CACCTCAGCA GGCAGCAGAG TGCCTCGCCG 
GCAGCCAACG ACTCGATTGC GGACAGCGAG GAGGAGGACT ACTTTGGCGC CGGTGCCCAG 
AATGGAAACA AACTGGGCTC CGAGAGCGAG GAGGAGACGC CGTTTGCATT TAGGCGCAGA 
TCAAGCTGTG TTTACCTACC GACTCGACAA CGAGATGGCC GCTATCCGTG GGACTCAGCC 
GACGAGGATA TGGCGCCCAG TGGTGCTTGT TCGGATGCTA AATACCGTTA CACACTGACC 
TCCCTCAATT ATCCCAGACC ACGCTGCATT GGCTTCGCAC GTCGTCGATT GGGTCGTGGC 
GGTCGCATCC TGCTGGACCG GGCCACAACG AATTTCGACG ACTTTTGGTC GCAGCTTGAC 
TACACGGTGA TGGAAAGCGT GACGGTCAAC AAATGTAGGG ATAAAATCAA GCAACGGGAT 
CCCGATCAGC TGTGCAAGCC CAGCTCACCG CCCACGGTGG TAGATCTGGT TCCCGCTGGA 
AAGCCCGTCG ATGGTGAGCA TCCTGTTCCT GAACTGATAA AGGAGGAGCC GTCAAGCGAT 
ACAGAAAAGC CTGTTGCTGC GACGGAATCT GGGCGGGAGA GCAAAGAACG GGAGCTTGAG 
GAGAAATTTG AGAACGATGT AGATGATGAA TTCGTAGCCA GTGACGATGA GAATGTGTCA 
CGGCTTGGAA TCTACAGTTC AGCGGTTACG TTACCCCAAA GTTGTGCTGA TCTTCGCCAG 
AAACGTCGCC GCCTGCGTCG CAAGAAGCAA CAGTTGCGAG AACTTAATGC CGCAAAGCGT 
CTAAAGAGGT CTTCGGAAGC GGTGGCAACC GCTGAAGATG TTCAACTGGA GGACGAGGAT 
ACTAAACCAG ATCTGCGTCC ATTACCCAGG GCTTACCTTC AGCGTTTGGC ATTGATTCTG 
GGACAAAAAC TAAAGGTGGA AGAGGATGAT AACACGGAAG TTTCCGAGAA TGTGGCGCCA 
GAGTTGCTGG AAATGAATGA TCCGGAGCAG CTGCAGCCCC CGCGAGCCAA TCATCAGCAA 
CACCACCGTT CCAACCACAA CAACAACAAC AATACCGTGT TGAACAACAA CAATAGTAGT 
AGTAGTAACG TTATGAATAA GGATGTTAGC ATTAGCGAAA AAATCAATGT GATAAAGCAG 
GAGGCGGAGG AGCTCGTCGG CGGCAAATGC GAGGATCAAC CGGTAGCTTC CACCTCGGCG 
GCAGCGGCCG CCGCTCGTGC CGCTGAAGCA GCCGCTGCAG CGGAGGCAGC CTCATCGACA 
ACCACTCTTG CGGCACCGAA TCCGGGTCCA TCGCTGGAGG AGGTGGCGAA GACGATAAAG 
CGCGAGATGA TCGATGCCGA CGACTCCAAC GAGCCCCTGA GCAGCATTCG TACAGCAGCT 
GTCATGCTGT CCACGCAGAC CTCTGTCTCC GAACTCATGG ACGATGAAGC TGAGGACGAT 
GTCAATCTCG CCCAGCTGAG CAGTATCATC CGGCATACGG CGGTCAAGAA GGAACTGGAG 
GCCCAGCAGC ACCAGCAACA AGTACAGCAT GAACAGATGC AGGCCGCCAG GAAGATGGAA 
GAGGATGAAG CCGCTCCCTG TCTGAACCAG CTGCCTGACG TGATTCGCTT GCGAAATATC 
CGCAACAGCC AAGTGCATGC GAGGCCGAAG GACCTGTTTG TACAAGCTCC TGTTGATGAA 
GAATTCAATG CAGACTACAT GGGTGGACTG TCGCCCACTT CCAGCCAGCG GCTAGACATC 
TGCAATGAGC TGCTTTCGGA GATCCGGCGC GATTGGCTAC ACTTCCGGCC CAAAACTCCC 
ACAGATGAGC TGTCCGATAG CCAAGATTGC GAAGTGGGTA AGACGTGTGA TAGTCACGCT 
GTCGACTGGA CGCAGGACAC GCCCATAAGT GTGGAACTCA ATCGCTTAGG AAAGCGAGAA 
GCAGAGGAAA GCGATTCTAG CTCGTACTTT TTGAGCAGTG GCTTTAAATA CAGGGATCTC 
GAGTCAGATG CCCAATTGCT GCAAACGGTC CAAGCTCAGG ACTACGCTCG AGGCAGCAGC 
AAGAGTCCAA ACTGCTCTGG GTCAGGCTCA GGATCTGCGC TAGAGTTTAA TCTCAGTGGT 
GGTGACTCCC TAAACGATAT CAACAACTTG TTGGGCTGTG ACGACGAGGA CGACAACCAC 
GAGCATATGC TAGACAACAT CCTGCAGGAG TGCGCCATGG ATGATCCCAA GGCATTGAAC 
CAAGCTACTA ATTTTTGGAA CGGTATCCTA GATGGCGAGG CAGCCGTGGA CGAAGTGGAA 
ATGGCCGATC AGCTGTTGGA TTGTATAGAC GAGAAGAAAT CTAAGGTGGG TGCAGCCGAT 
ACATCGAAGC GCGCGGGTCG CAACCGCAAG GCAAGATTGA TCCAAGCTCC AGTGGGTAGC 
TCCGCATTTA CTGTGTGTCC CACGGCGGAG ACTCTGAAGG AACGTGAACT TTTCTTTAGT 
CAAGCTCCGC CTGTTGAAGT CCCGCCCAAA GAGAAAACGC CAGCAGAAGT TGAACTGCCG 
GAAGTTGTGG TCAAGGAAAC AGCGAATTTG CCTGTGAAGG TTGAGGCGGT TGAGAAACCC 
CAGTCGGAGG TTCTTCCCGT TGCCCCACAG CCGCAACAGC TTCAGCCGCA GGCTATTACT 
TTGCCCACGA CGCAGCTGAT CAACATACCC GCCCAGATAA GCACGCAAAA GCAGCATCAG 
CAGCCACAGG TGACGCAATT GCTCAATCAG TTTGCCAACG CTGGTCCGCA GATCATCGCC 
CGCACAGAAT ACGGCGGAGG AGCAGCTGTG GGCGATATAG TGACCATAAC CCCGCACACG 
GGGAGCAGTC CACTCCCCAC ACTCACCGCC CAGCAACAAC TGCAGCACCA ATTGGCACAG 
GCTCAACTCC GAAATGTGAC CGTCCAGCAG CGACAGGCGA CACCTCAAAA CCCCAATCAA 
AATCAGAATC CCCAGCAGCA GTTACTCTTC CAAATGCAGC GGGATCCCTC GCGCTCCCTG 
ACGCCCCTAC AAGCTGCCGG CGCTGGCGCC ACTGGCAACC ATTTGATCTA CACAACAAGC 
TGCGGCGAGA TTGTCACAGC AGGAGCTGCA GCTTCTGCGT CTCAGAAGGT CACATATGCC 
ATCCAGAAAG CCGGTGTCTC AAGTGGAGGA GCAACCACCG CCTCAATTGG ACCCAACACA 
GTAATCACGC TGTCCAATGT AAAGGTGCAG AACGATGATA TCTCCTCTGG TGGTAGTGGC 
AACTCAGGAT CCAGTGTGAA CATCATCAAC ACGGCCAGTG GCCAGCAGTT AGCCGTGCAC 
AGCATTGCCG GTATGCAACA GCAGGGCCAG CAACAGGTGC AACAGGTGAC CACCAGTACG 
CCTTTGCTTG TCGCCACACC CACTCGCAAC AATGTGCAGC AGCAACAGCA GCAGTTGGTG 
CAGCAGCAAC CGATTGTTTG GCGGCAGGTG AGCGGAACGA ACACGGCGGT GGCCACCACA 
GCAGTGCTCT CCACCACGGT GGACTCCTCG CCGAAGGCGG TAACCAACCA GATCCTCTGG 
ACCAGCCGGG CCACGCAAAA AAGGCAGCTC AACGGGCCAG AGAACACAGA CATCAACAAA 
CTTCTTCTCA ATCGGAAGTT GCCGCGCAAG CAGCAAATAC AACAACAGCA CCAACAGATT 
CAACTGACAA AGCAGCAGCT TCAACAATTG GTGCAGCATG AGGAGTTGGA TGACTTGGTG 
GCCACATCGA CGGGCAACAG TGGCGATCAG ATGGAAACCG GCGACGGCCT GCAACAACAA 
CAGCAACAGC AGCAACAACA GCAGCAGCTG CAGAAGCTCA GCAAGGTGAT CAAGATGTCG 
CAGTTTCCAC TGCTCGTGTC GGATGTAAGT CTGCAGCAGC AGCAGTCGGT GTCTGGCCAA 
GCTCAGCAGC AACAATTAAA GCCCAACTCT GCGGCGGCGA TTGCAGCTAA TCACAACTAC 
AGCCTGCACC CTGCCTCAAC GGCGATCATC ACCGCCCAGG CGACACCCCA GCAGGCCAAC 
AAGATCTTCT TGACCAGCAG CAACAGTGGT GGCGGCGGAT CCGCTGGTAA CTTTACCATC 
GCCACTGCCA GCGAACTGCA GGCGGCGGCA GCCGGCCAAA AGCTTCACTA CAAGGAGCTG 
CCCAACTCCA ACCTCAAGCT GAACTTTGTG AGCAGTGCAG GGACGGGTGA GCAAACGCAG 
CAGGTGGGCG CCACAACAGT GCTGCTGAAC AAATCCCAAC AGCAGCAGCA ACAACAGCAG 
CATCAAAAGC TGAAAACGTT GGCCACTACA GGAAATTCTC AAACTGGCCA GGGCGATAAG 
CGAGTGCTGT ACACCAGTCT GCTGAATGTG AAACCGCTGC AGAAGAACAA CAGTGGTCAG 
ATGCAGATGC AACTGGGACC GGGAGGATTG CAACAGGTGC TCCGTGGACG ACTCAACAAT 
TCAGCGATCA TTACTAGAAC GCTGCCGCTG GGCACAACTG TTAACAGTAC CGGCGGTGCT 
GTAGGTCCTA CCACCACGAC TATTAGGCAG TTGGTTACCA CGAATGCGAA CAACGTGAGC 
AGCCAAGATG GCAACCTACT TAGTGCAAAG GAAGTGGGGA AAGCCCTGAC AGTGGAGCAG 
GTGATAGCCA GCGCTAACAA CGGAGGAGTT GTGCTGCCCA CGAGCAACAA CAATAATAAC 
AACAATGGCA GTGGAGCTGG CGGAAATGGA GGACCTTTGA ACAGCGGTGT CAATGCCACA 
GGAGCTGGCG GAGGAGGATC TACAGGCGCG GGTGTTGGGG GAGCTGGTGT GACGTCAACC 
ATCAACAGAT GAGACGCGGA TGGCAGCGGC AGCAACAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(Pc) ( NM_001273966.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260895.1 Drosophila melanogaster enhancer of polycomb, transcript variant C (E(Pc)), mRNA.

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CloneID	OFa12169
Clone ID Related Accession (Same CDS sequence)	NM_001273966.2
Accession Version	NM_001273966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of polycomb, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001273966.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GGTGCGTTTT CGGACACCTT TTACTTATGA AGAAAGATGC CATCAGTGGC 
AGTGGGAATG GAAACAGCAG TCGCAAGGAG AAGCGTCCAT ACAAGAAGCG CAAGCACAAA 
TTGCCCCGCG ACAAGCAGCA GCACCAGCAG CAGCAAACGC AGCAGCACCA GCAACAACAG 
TATTTGCCGG CGTCTGGTGG CGCCGGAGTC TCGCCCGCGC ACCACCTGCC CCACCATCTG 
CACCACCTCA GCAGGCAGCA GAGTGCCTCG CCGGCAGCCA ACGACTCGAT TGCGGACAGC 
GAGGAGGAGG ACTACTTTGG CGCCGGTGCC CAGAATGGAA ACAAACTGGG CTCCGAGAGC 
GAGGAGGAGA CGCCGTTTGC ATTTAGGCGC AGATCAAGCT GTGTTTACCT ACCGACTCGA 
CAACGAGATG GCCGCTATCC GTGGGACTCA GCCGACGAGG ATATGGCGCC CAGTGGTGCT 
TGTTCGGATG CTAAATACCG TTACACACTG ACCTCCCTCA ATTATCCCAG ACCACGCTGC 
ATTGGCTTCG CACGTCGTCG ATTGGGTCGT GGCGGTCGCA TCCTGCTGGA CCGGGCCACA 
ACGAATTTCG ACGACTTTTG GTCGCAGCTT GACTACACGG TGATGGAAAG CGTGACGGTC 
AACAAATGTA GGGATAAAAT CAAGCAACGG GATCCCGATC AGCTGTGCAA GCCCAGCTCA 
CCGCCCACGG TGGTAGATCT GGTTCCCGCT GGAAAGCCCG TCGATGGTGA GCATCCTGTT 
CCTGAACTGA TAAAGGAGGA GCCGTCAAGC GATACAGAAA AGCCTGTTGC TGCGACGGAA 
TCTGGGCGGG AGAGCAAAGA ACGGGAGCTT GAGGAGAAAT TTGAGAACGA TGTAGATGAT 
GAATTCGTAG CCAGTGACGA TGAGAATGTG TCACGGCTTG GAATCTACAG TTCAGCGGTT 
ACGTTACCCC AAAGTTGTGC TGATCTTCGC CAGAAACGTC GCCGCCTGCG TCGCAAGAAG 
CAACAGTTGC GAGAACTTAA TGCCGCAAAG CGTCTAAAGA GGTCTTCGGA AGCGGTGGCA 
ACCGCTGAAG ATGTTCAACT GGAGGACGAG GATACTAAAC CAGATCTGCG TCCATTACCC 
AGGGCTTACC TTCAGCGTTT GGCATTGATT CTGGGACAAA AACTAAAGGT GGAAGAGGAT 
GATAACACGG AAGTTTCCGA GAATGTGGCG CCAGAGTTGC TGGAAATGAA TGATCCGGAG 
CAGCTGCAGC CCCCGCGAGC CAATCATCAG CAACACCACC GTTCCAACCA CAACAACAAC 
AACAATACCG TGTTGAACAA CAACAATAGT AGTAGTAGTA ACGTTATGAA TAAGGATGTT 
AGCATTAGCG AAAAAATCAA TGTGATAAAG CAGGAGGCGG AGGAGCTCGT CGGCGGCAAA 
TGCGAGGATC AACCGGTAGC TTCCACCTCG GCGGCAGCGG CCGCCGCTCG TGCCGCTGAA 
GCAGCCGCTG CAGCGGAGGC AGCCTCATCG ACAACCACTC TTGCGGCACC GAATCCGGGT 
CCATCGCTGG AGGAGGTGGC GAAGACGATA AAGCGCGAGA TGATCGATGC CGACGACTCC 
AACGAGCCCC TGAGCAGCAT TCGTACAGCA GCTGTCATGC TGTCCACGCA GACCTCTGTC 
TCCGAACTCA TGGACGATGA AGCTGAGGAC GATGTCAATC TCGCCCAGCT GAGCAGTATC 
ATCCGGCATA CGGCGGTCAA GAAGGAACTG GAGGCCCAGC AGCACCAGCA ACAAGTACAG 
CATGAACAGA TGCAGGCCGC CAGGAAGATG GAAGAGGATG AAGCCGCTCC CTGTCTGAAC 
CAGCTGCCTG ACGTGATTCG CTTGCGAAAT ATCCGCAACA GCCAAGTGCA TGCGAGGCCG 
AAGGACCTGT TTGTACAAGC TCCTGTTGAT GAAGAATTCA ATGCAGACTA CATGGGTGGA 
CTGTCGCCCA CTTCCAGCCA GCGGCTAGAC ATCTGCAATG AGCTGCTTTC GGAGATCCGG 
CGCGATTGGC TACACTTCCG GCCCAAAACT CCCACAGATG AGCTGTCCGA TAGCCAAGAT 
TGCGAAGTGG GTAAGACGTG TGATAGTCAC GCTGTCGACT GGACGCAGGA CACGCCCATA 
AGTGTGGAAC TCAATCGCTT AGGAAAGCGA GAAGCAGAGG AAAGCGATTC TAGCTCGTAC 
TTTTTGAGCA GTGGCTTTAA ATACAGGGAT CTCGAGTCAG ATGCCCAATT GCTGCAAACG 
GTCCAAGCTC AGGACTACGC TCGAGGCAGC AGCAAGAGTC CAAACTGCTC TGGGTCAGGC 
TCAGGATCTG CGCTAGAGTT TAATCTCAGT GGTGGTGACT CCCTAAACGA TATCAACAAC 
TTGTTGGGCT GTGACGACGA GGACGACAAC CACGAGCATA TGCTAGACAA CATCCTGCAG 
GAGTGCGCCA TGGATGATCC CAAGGCATTG AACCAAGCTA CTAATTTTTG GAACGGTATC 
CTAGATGGCG AGGCAGCCGT GGACGAAGTG GAAATGGCCG ATCAGCTGTT GGATTGTATA 
GACGAGAAGA AATCTAAGGT GGGTGCAGCC GATACATCGA AGCGCGCGGG TCGCAACCGC 
AAGGCAAGAT TGATCCAAGC TCCAGTGGGT AGCTCCGCAT TTACTGTGTG TCCCACGGCG 
GAGACTCTGA AGGAACGTGA ACTTTTCTTT AGTCAAGCTC CGCCTGTTGA AGTCCCGCCC 
AAAGAGAAAA CGCCAGCAGA AGTTGAACTG CCGGAAGTTG TGGTCAAGGA AACAGCGAAT 
TTGCCTGTGA AGGTTGAGGC GGTTGAGAAA CCCCAGTCGG AGGTTCTTCC CGTTGCCCCA 
CAGCCGCAAC AGCTTCAGCC GCAGGCTATT ACTTTGCCCA CGACGCAGCT GATCAACATA 
CCCGCCCAGA TAAGCACGCA AAAGCAGCAT CAGCAGCCAC AGGTGACGCA ATTGCTCAAT 
CAGTTTGCCA ACGCTGGTCC GCAGATCATC GCCCGCACAG AATACGGCGG AGGAGCAGCT 
GTGGGCGATA TAGTGACCAT AACCCCGCAC ACGGGGAGCA GTCCACTCCC CACACTCACC 
GCCCAGCAAC AACTGCAGCA CCAATTGGCA CAGGCTCAAC TCCGAAATGT GACCGTCCAG 
CAGCGACAGG CGACACCTCA AAACCCCAAT CAAAATCAGA ATCCCCAGCA GCAGTTACTC 
TTCCAAATGC AGCGGGATCC CTCGCGCTCC CTGACGCCCC TACAAGCTGC CGGCGCTGGC 
GCCACTGGCA ACCATTTGAT CTACACAACA AGCTGCGGCG AGATTGTCAC AGCAGGAGCT 
GCAGCTTCTG CGTCTCAGAA GGTCACATAT GCCATCCAGA AAGCCGGTGT CTCAAGTGGA 
GGAGCAACCA CCGCCTCAAT TGGACCCAAC ACAGTAATCA CGCTGTCCAA TGTAAAGGTG 
CAGAACGATG ATATCTCCTC TGGTGGTAGT GGCAACTCAG GATCCAGTGT GAACATCATC 
AACACGGCCA GTGGCCAGCA GTTAGCCGTG CACAGCATTG CCGGTATGCA ACAGCAGGGC 
CAGCAACAGG TGCAACAGGT GACCACCAGT ACGCCTTTGC TTGTCGCCAC ACCCACTCGC 
AACAATGTGC AGCAGCAACA GCAGCAGTTG GTGCAGCAGC AACCGATTGT TTGGCGGCAG 
GTGAGCGGAA CGAACACGGC GGTGGCCACC ACAGCAGTGC TCTCCACCAC GGTGGACTCC 
TCGCCGAAGG CGGTAACCAA CCAGATCCTC TGGACCAGCC GGGCCACGCA AAAAAGGCAG 
CTCAACGGGC CAGAGAACAC AGACATCAAC AAACTTCTTC TCAATCGGAA GTTGCCGCGC 
AAGCAGCAAA TACAACAACA GCACCAACAG ATTCAACTGA CAAAGCAGCA GCTTCAACAA 
TTGGTGCAGC ATGAGGAGTT GGATGACTTG GTGGCCACAT CGACGGGCAA CAGTGGCGAT 
CAGATGGAAA CCGGCGACGG CCTGCAACAA CAACAGCAAC AGCAGCAACA ACAGCAGCAG 
CTGCAGAAGC TCAGCAAGGT GATCAAGATG TCGCAGTTTC CACTGCTCGT GTCGGATGTA 
AGTCTGCAGC AGCAGCAGTC GGTGTCTGGC CAAGCTCAGC AGCAACAATT AAAGCCCAAC 
TCTGCGGCGG CGATTGCAGC TAATCACAAC TACAGCCTGC ACCCTGCCTC AACGGCGATC 
ATCACCGCCC AGGCGACACC CCAGCAGGCC AACAAGATCT TCTTGACCAG CAGCAACAGT 
GGTGGCGGCG GATCCGCTGG TAACTTTACC ATCGCCACTG CCAGCGAACT GCAGGCGGCG 
GCAGCCGGCC AAAAGCTTCA CTACAAGGAG CTGCCCAACT CCAACCTCAA GCTGAACTTT 
GTGAGCAGTG CAGGGACGGG TGAGCAAACG CAGCAGGTGG GCGCCACAAC AGTGCTGCTG 
AACAAATCCC AACAGCAGCA GCAACAACAG CAGCATCAAA AGCTGAAAAC GTTGGCCACT 
ACAGGAAATT CTCAAACTGG CCAGGGCGAT AAGCGAGTGC TGTACACCAG TCTGCTGAAT 
GTGAAACCGC TGCAGAAGAA CAACAGTGGT CAGATGCAGA TGCAACTGGG ACCGGGAGGA 
TTGCAACAGG TGCTCCGTGG ACGACTCAAC AATTCAGCGA TCATTACTAG AACGCTGCCG 
CTGGGCACAA CTGTTAACAG TACCGGCGGT GCTGTAGGTC CTACCACCAC GACTATTAGG 
CAGTTGGTTA CCACGAATGC GAACAACGTG AGCAGCCAAG ATGGCAACCT ACTTAGTGCA 
AAGGAAGTGG GGAAAGCCCT GACAGTGGAG CAGGTGATAG CCAGCGCTAA CAACGGAGGA 
GTTGTGCTGC CCACGAGCAA CAACAATAAT AACAACAATG GCAGTGGAGC TGGCGGAAAT 
GGAGGACCTT TGAACAGCGG TGTCAATGCC ACAGGAGCTG GCGGAGGAGG ATCTACAGGC 
GCGGGTGTTG GGGGAGCTGG TGTGACGTCA ACCATCAACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260895.1
CDS	1042..6966
Translation	MSKLSFRARHLDPSKQMPIYLAEELPDLPEYSAINRAVPQMPSGMEKEEESEHHLQRAICTGLIIPTPEVLQTDQPFYDAYYPPDYKMPRQMIHMQPLGLDTEVPDYDMDSADEDWLSQQQRLELTELKFEQMMDRLEKSSGQTVVTLNEAKSLLNQDDETSISVYDYWLNKRLKMQHPLILTVKTESRPGASSNNPYLAFRRRTEKMQTRKNRKNDEASYEKMLKLRRDLQRATTILEMVRRREETKRDHLKMTVNIFEKRVEMRDFNGAVYSELNSQYKNTRCVFGHLLLMKKDAISGSGNGNSSRKEKRPYKKRKHKLPRDKQQHQQQQTQQHQQQQYLPASGGAGVSPAHHLPHHLHHLSRQQSASPAANDSIADSEEEDYFGAGAQNGNKLGSESEEETPFAFRRRSSCVYLPTRQRDGRYPWDSADEDMAPSGACSDAKYRYTLTSLNYPRPRCIGFARRRLGRGGRILLDRATTNFDDFWSQLDYTVMESVTVNKCRDKIKQRDPDQLCKPSSPPTVVDLVPAGKPVDGEHPVPELIKEEPSSDTEKPVAATESGRESKERELEEKFENDVDDEFVASDDENVSRLGIYSSAVTLPQSCADLRQKRRRLRRKKQQLRELNAAKRLKRSSEAVATAEDVQLEDEDTKPDLRPLPRAYLQRLALILGQKLKVEEDDNTEVSENVAPELLEMNDPEQLQPPRANHQQHHRSNHNNNNNTVLNNNNSSSSNVMNKDVSISEKINVIKQEAEELVGGKCEDQPVASTSAAAAAARAAEAAAAAEAASSTTTLAAPNPGPSLEEVAKTIKREMIDADDSNEPLSSIRTAAVMLSTQTSVSELMDDEAEDDVNLAQLSSIIRHTAVKKELEAQQHQQQVQHEQMQAARKMEEDEAAPCLNQLPDVIRLRNIRNSQVHARPKDLFVQAPVDEEFNADYMGGLSPTSSQRLDICNELLSEIRRDWLHFRPKTPTDELSDSQDCEVGKTCDSHAVDWTQDTPISVELNRLGKREAEESDSSSYFLSSGFKYRDLESDAQLLQTVQAQDYARGSSKSPNCSGSGSGSALEFNLSGGDSLNDINNLLGCDDEDDNHEHMLDNILQECAMDDPKALNQATNFWNGILDGEAAVDEVEMADQLLDCIDEKKSKVGAADTSKRAGRNRKARLIQAPVGSSAFTVCPTAETLKERELFFSQAPPVEVPPKEKTPAEVELPEVVVKETANLPVKVEAVEKPQSEVLPVAPQPQQLQPQAITLPTTQLINIPAQISTQKQHQQPQVTQLLNQFANAGPQIIARTEYGGGAAVGDIVTITPHTGSSPLPTLTAQQQLQHQLAQAQLRNVTVQQRQATPQNPNQNQNPQQQLLFQMQRDPSRSLTPLQAAGAGATGNHLIYTTSCGEIVTAGAAASASQKVTYAIQKAGVSSGGATTASIGPNTVITLSNVKVQNDDISSGGSGNSGSSVNIINTASGQQLAVHSIAGMQQQGQQQVQQVTTSTPLLVATPTRNNVQQQQQQLVQQQPIVWRQVSGTNTAVATTAVLSTTVDSSPKAVTNQILWTSRATQKRQLNGPENTDINKLLLNRKLPRKQQIQQQHQQIQLTKQQLQQLVQHEELDDLVATSTGNSGDQMETGDGLQQQQQQQQQQQQLQKLSKVIKMSQFPLLVSDVSLQQQQSVSGQAQQQQLKPNSAAAIAANHNYSLHPASTAIITAQATPQQANKIFLTSSNSGGGGSAGNFTIATASELQAAAAGQKLHYKELPNSNLKLNFVSSAGTGEQTQQVGATTVLLNKSQQQQQQQQHQKLKTLATTGNSQTGQGDKRVLYTSLLNVKPLQKNNSGQMQMQLGPGGLQQVLRGRLNNSAIITRTLPLGTTVNSTGGAVGPTTTTIRQLVTTNANNVSSQDGNLLSAKEVGKALTVEQVIASANNGGVVLPTSNNNNNNNGSGAGGNGGPLNSGVNATGAGGGGSTGAGVGGAGVTSTINR

Target ORF information:

RefSeq Version	NM_001273966.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of polycomb, transcript variant C (E(Pc)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273966.2

ORF Insert Sequence:

ATGTCCAAGC TGTCGTTCAG AGCCCGCCAT TTGGATCCGT CCAAGCAGAT GCCCATCTAC 
TTGGCGGAGG AGCTGCCCGA CCTCCCCGAG TACTCAGCCA TCAACCGGGC AGTGCCTCAA 
ATGCCCAGTG GCATGGAGAA GGAGGAGGAG TCGGAGCACC ACTTGCAGCG AGCGATATGC 
ACGGGTCTGA TCATCCCCAC GCCCGAGGTG CTGCAGACGG ATCAGCCCTT CTACGATGCC 
TACTATCCGC CGGACTACAA AATGCCCCGC CAAATGATCC ACATGCAACC TCTTGGGCTG 
GATACCGAGG TCCCTGACTA CGACATGGAC AGTGCCGATG AGGACTGGCT CAGTCAGCAG 
CAGCGCCTGG AGCTTACCGA ACTCAAGTTC GAGCAGATGA TGGACCGGCT AGAAAAGAGC 
TCCGGCCAAA CGGTGGTCAC GCTGAACGAG GCCAAGTCGC TGCTTAACCA GGACGACGAG 
ACCAGCATAT CCGTCTACGA TTACTGGCTG AACAAACGCC TCAAGATGCA ACACCCGCTG 
ATCCTCACTG TGAAAACTGA GAGTCGACCA GGCGCCAGCT CCAACAATCC GTACTTGGCT 
TTTCGTCGTC GCACCGAGAA AATGCAAACC CGCAAAAATC GAAAGAACGA TGAGGCCTCC 
TACGAAAAGA TGCTCAAGCT GCGACGTGAC CTGCAGCGCG CCACCACTAT ACTGGAGATG 
GTGAGGCGAC GCGAGGAGAC GAAGCGCGAT CATCTCAAGA TGACGGTGAA CATCTTCGAG 
AAGCGCGTTG AGATGCGCGA CTTCAACGGT GCTGTCTACT CCGAGCTGAA TTCTCAGTAC 
AAGAACACAA GGTGCGTTTT CGGACACCTT TTACTTATGA AGAAAGATGC CATCAGTGGC 
AGTGGGAATG GAAACAGCAG TCGCAAGGAG AAGCGTCCAT ACAAGAAGCG CAAGCACAAA 
TTGCCCCGCG ACAAGCAGCA GCACCAGCAG CAGCAAACGC AGCAGCACCA GCAACAACAG 
TATTTGCCGG CGTCTGGTGG CGCCGGAGTC TCGCCCGCGC ACCACCTGCC CCACCATCTG 
CACCACCTCA GCAGGCAGCA GAGTGCCTCG CCGGCAGCCA ACGACTCGAT TGCGGACAGC 
GAGGAGGAGG ACTACTTTGG CGCCGGTGCC CAGAATGGAA ACAAACTGGG CTCCGAGAGC 
GAGGAGGAGA CGCCGTTTGC ATTTAGGCGC AGATCAAGCT GTGTTTACCT ACCGACTCGA 
CAACGAGATG GCCGCTATCC GTGGGACTCA GCCGACGAGG ATATGGCGCC CAGTGGTGCT 
TGTTCGGATG CTAAATACCG TTACACACTG ACCTCCCTCA ATTATCCCAG ACCACGCTGC 
ATTGGCTTCG CACGTCGTCG ATTGGGTCGT GGCGGTCGCA TCCTGCTGGA CCGGGCCACA 
ACGAATTTCG ACGACTTTTG GTCGCAGCTT GACTACACGG TGATGGAAAG CGTGACGGTC 
AACAAATGTA GGGATAAAAT CAAGCAACGG GATCCCGATC AGCTGTGCAA GCCCAGCTCA 
CCGCCCACGG TGGTAGATCT GGTTCCCGCT GGAAAGCCCG TCGATGGTGA GCATCCTGTT 
CCTGAACTGA TAAAGGAGGA GCCGTCAAGC GATACAGAAA AGCCTGTTGC TGCGACGGAA 
TCTGGGCGGG AGAGCAAAGA ACGGGAGCTT GAGGAGAAAT TTGAGAACGA TGTAGATGAT 
GAATTCGTAG CCAGTGACGA TGAGAATGTG TCACGGCTTG GAATCTACAG TTCAGCGGTT 
ACGTTACCCC AAAGTTGTGC TGATCTTCGC CAGAAACGTC GCCGCCTGCG TCGCAAGAAG 
CAACAGTTGC GAGAACTTAA TGCCGCAAAG CGTCTAAAGA GGTCTTCGGA AGCGGTGGCA 
ACCGCTGAAG ATGTTCAACT GGAGGACGAG GATACTAAAC CAGATCTGCG TCCATTACCC 
AGGGCTTACC TTCAGCGTTT GGCATTGATT CTGGGACAAA AACTAAAGGT GGAAGAGGAT 
GATAACACGG AAGTTTCCGA GAATGTGGCG CCAGAGTTGC TGGAAATGAA TGATCCGGAG 
CAGCTGCAGC CCCCGCGAGC CAATCATCAG CAACACCACC GTTCCAACCA CAACAACAAC 
AACAATACCG TGTTGAACAA CAACAATAGT AGTAGTAGTA ACGTTATGAA TAAGGATGTT 
AGCATTAGCG AAAAAATCAA TGTGATAAAG CAGGAGGCGG AGGAGCTCGT CGGCGGCAAA 
TGCGAGGATC AACCGGTAGC TTCCACCTCG GCGGCAGCGG CCGCCGCTCG TGCCGCTGAA 
GCAGCCGCTG CAGCGGAGGC AGCCTCATCG ACAACCACTC TTGCGGCACC GAATCCGGGT 
CCATCGCTGG AGGAGGTGGC GAAGACGATA AAGCGCGAGA TGATCGATGC CGACGACTCC 
AACGAGCCCC TGAGCAGCAT TCGTACAGCA GCTGTCATGC TGTCCACGCA GACCTCTGTC 
TCCGAACTCA TGGACGATGA AGCTGAGGAC GATGTCAATC TCGCCCAGCT GAGCAGTATC 
ATCCGGCATA CGGCGGTCAA GAAGGAACTG GAGGCCCAGC AGCACCAGCA ACAAGTACAG 
CATGAACAGA TGCAGGCCGC CAGGAAGATG GAAGAGGATG AAGCCGCTCC CTGTCTGAAC 
CAGCTGCCTG ACGTGATTCG CTTGCGAAAT ATCCGCAACA GCCAAGTGCA TGCGAGGCCG 
AAGGACCTGT TTGTACAAGC TCCTGTTGAT GAAGAATTCA ATGCAGACTA CATGGGTGGA 
CTGTCGCCCA CTTCCAGCCA GCGGCTAGAC ATCTGCAATG AGCTGCTTTC GGAGATCCGG 
CGCGATTGGC TACACTTCCG GCCCAAAACT CCCACAGATG AGCTGTCCGA TAGCCAAGAT 
TGCGAAGTGG GTAAGACGTG TGATAGTCAC GCTGTCGACT GGACGCAGGA CACGCCCATA 
AGTGTGGAAC TCAATCGCTT AGGAAAGCGA GAAGCAGAGG AAAGCGATTC TAGCTCGTAC 
TTTTTGAGCA GTGGCTTTAA ATACAGGGAT CTCGAGTCAG ATGCCCAATT GCTGCAAACG 
GTCCAAGCTC AGGACTACGC TCGAGGCAGC AGCAAGAGTC CAAACTGCTC TGGGTCAGGC 
TCAGGATCTG CGCTAGAGTT TAATCTCAGT GGTGGTGACT CCCTAAACGA TATCAACAAC 
TTGTTGGGCT GTGACGACGA GGACGACAAC CACGAGCATA TGCTAGACAA CATCCTGCAG 
GAGTGCGCCA TGGATGATCC CAAGGCATTG AACCAAGCTA CTAATTTTTG GAACGGTATC 
CTAGATGGCG AGGCAGCCGT GGACGAAGTG GAAATGGCCG ATCAGCTGTT GGATTGTATA 
GACGAGAAGA AATCTAAGGT GGGTGCAGCC GATACATCGA AGCGCGCGGG TCGCAACCGC 
AAGGCAAGAT TGATCCAAGC TCCAGTGGGT AGCTCCGCAT TTACTGTGTG TCCCACGGCG 
GAGACTCTGA AGGAACGTGA ACTTTTCTTT AGTCAAGCTC CGCCTGTTGA AGTCCCGCCC 
AAAGAGAAAA CGCCAGCAGA AGTTGAACTG CCGGAAGTTG TGGTCAAGGA AACAGCGAAT 
TTGCCTGTGA AGGTTGAGGC GGTTGAGAAA CCCCAGTCGG AGGTTCTTCC CGTTGCCCCA 
CAGCCGCAAC AGCTTCAGCC GCAGGCTATT ACTTTGCCCA CGACGCAGCT GATCAACATA 
CCCGCCCAGA TAAGCACGCA AAAGCAGCAT CAGCAGCCAC AGGTGACGCA ATTGCTCAAT 
CAGTTTGCCA ACGCTGGTCC GCAGATCATC GCCCGCACAG AATACGGCGG AGGAGCAGCT 
GTGGGCGATA TAGTGACCAT AACCCCGCAC ACGGGGAGCA GTCCACTCCC CACACTCACC 
GCCCAGCAAC AACTGCAGCA CCAATTGGCA CAGGCTCAAC TCCGAAATGT GACCGTCCAG 
CAGCGACAGG CGACACCTCA AAACCCCAAT CAAAATCAGA ATCCCCAGCA GCAGTTACTC 
TTCCAAATGC AGCGGGATCC CTCGCGCTCC CTGACGCCCC TACAAGCTGC CGGCGCTGGC 
GCCACTGGCA ACCATTTGAT CTACACAACA AGCTGCGGCG AGATTGTCAC AGCAGGAGCT 
GCAGCTTCTG CGTCTCAGAA GGTCACATAT GCCATCCAGA AAGCCGGTGT CTCAAGTGGA 
GGAGCAACCA CCGCCTCAAT TGGACCCAAC ACAGTAATCA CGCTGTCCAA TGTAAAGGTG 
CAGAACGATG ATATCTCCTC TGGTGGTAGT GGCAACTCAG GATCCAGTGT GAACATCATC 
AACACGGCCA GTGGCCAGCA GTTAGCCGTG CACAGCATTG CCGGTATGCA ACAGCAGGGC 
CAGCAACAGG TGCAACAGGT GACCACCAGT ACGCCTTTGC TTGTCGCCAC ACCCACTCGC 
AACAATGTGC AGCAGCAACA GCAGCAGTTG GTGCAGCAGC AACCGATTGT TTGGCGGCAG 
GTGAGCGGAA CGAACACGGC GGTGGCCACC ACAGCAGTGC TCTCCACCAC GGTGGACTCC 
TCGCCGAAGG CGGTAACCAA CCAGATCCTC TGGACCAGCC GGGCCACGCA AAAAAGGCAG 
CTCAACGGGC CAGAGAACAC AGACATCAAC AAACTTCTTC TCAATCGGAA GTTGCCGCGC 
AAGCAGCAAA TACAACAACA GCACCAACAG ATTCAACTGA CAAAGCAGCA GCTTCAACAA 
TTGGTGCAGC ATGAGGAGTT GGATGACTTG GTGGCCACAT CGACGGGCAA CAGTGGCGAT 
CAGATGGAAA CCGGCGACGG CCTGCAACAA CAACAGCAAC AGCAGCAACA ACAGCAGCAG 
CTGCAGAAGC TCAGCAAGGT GATCAAGATG TCGCAGTTTC CACTGCTCGT GTCGGATGTA 
AGTCTGCAGC AGCAGCAGTC GGTGTCTGGC CAAGCTCAGC AGCAACAATT AAAGCCCAAC 
TCTGCGGCGG CGATTGCAGC TAATCACAAC TACAGCCTGC ACCCTGCCTC AACGGCGATC 
ATCACCGCCC AGGCGACACC CCAGCAGGCC AACAAGATCT TCTTGACCAG CAGCAACAGT 
GGTGGCGGCG GATCCGCTGG TAACTTTACC ATCGCCACTG CCAGCGAACT GCAGGCGGCG 
GCAGCCGGCC AAAAGCTTCA CTACAAGGAG CTGCCCAACT CCAACCTCAA GCTGAACTTT 
GTGAGCAGTG CAGGGACGGG TGAGCAAACG CAGCAGGTGG GCGCCACAAC AGTGCTGCTG 
AACAAATCCC AACAGCAGCA GCAACAACAG CAGCATCAAA AGCTGAAAAC GTTGGCCACT 
ACAGGAAATT CTCAAACTGG CCAGGGCGAT AAGCGAGTGC TGTACACCAG TCTGCTGAAT 
GTGAAACCGC TGCAGAAGAA CAACAGTGGT CAGATGCAGA TGCAACTGGG ACCGGGAGGA 
TTGCAACAGG TGCTCCGTGG ACGACTCAAC AATTCAGCGA TCATTACTAG AACGCTGCCG 
CTGGGCACAA CTGTTAACAG TACCGGCGGT GCTGTAGGTC CTACCACCAC GACTATTAGG 
CAGTTGGTTA CCACGAATGC GAACAACGTG AGCAGCCAAG ATGGCAACCT ACTTAGTGCA 
AAGGAAGTGG GGAAAGCCCT GACAGTGGAG CAGGTGATAG CCAGCGCTAA CAACGGAGGA 
GTTGTGCTGC CCACGAGCAA CAACAATAAT AACAACAATG GCAGTGGAGC TGGCGGAAAT 
GGAGGACCTT TGAACAGCGG TGTCAATGCC ACAGGAGCTG GCGGAGGAGG ATCTACAGGC 
GCGGGTGTTG GGGGAGCTGG TGTGACGTCA ACCATCAACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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E(Pc) cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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