dgo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dgo
Entrez Gene ID	36139
Full Name	diego
Synonyms	CG12342,Dgo,Dmel\CG12342,EP(2)2619,EP2619
General protein information	Preferred Names diego Names CG12342 gene product from transcript CG12342-RA CG12342-PA CG12342-PB EP2619 dgo-PA dgo-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47C1-47C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_136770.2	NP_610614.1	diego, isoform A
NM_165800.2	NP_724973.1	diego, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicated that mAnkrd6 is a functional homolog of the Drosophila diego gene for mammalian planar cell polarity (PCP) regulation.
Title: Ankrd6 is a mammalian functional homolog of Drosophila planar cell polarity gene diego and regulates coordinated cellular orientation in the mouse inner ear.

demonstrate that Diversin and Dishevelled function together in a mutually dependent fashion in zebrafish gastrulation and organ formation
Title: Diversin regulates heart formation and gastrulation movements in development.

the initial membrane association of Dgo depends on Fz, and that Dgo physically interacts with Strabismus and prickle through its Ankyrin repeats
Title: Diego interacts with Prickle and Strabismus/Van Gogh to localize planar cell polarity complexes.

The following dgo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12913	NM_165800.2 Latest version!	Drosophila melanogaster diego (dgo), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa12913	NM_136770.2 Latest version!	Drosophila melanogaster diego (dgo), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dgo ( NM_136770.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610614.1 Drosophila melanogaster diego (dgo), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12913
Clone ID Related Accession (Same CDS sequence)	NM_136770.2 , NM_165800.2
Accession Version	NM_136770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diego, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136770.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCATG GATCCTCCCG CATTCCGAAA CAGGAAGCTC TCCCGAATGC GATATCCGGG 
CCCGACTCCA GCAATTGGAC TTCGCTGCAC GAGGCGGTAG CCGCCGGAGA TGATCGCCGG 
GTGGAGGGTC TCCTGTTCAG CAATGCTGAT CGACTGGCCA GGGAATCCGT GCAGGGGAAT 
ACCCCGCTCC ATGAGGCCGC CAGTCGGGGG TTCAGTCGCT GCGTGAAATT GATCTGCACG 
CCCCCCACGC CACCCGCTTC CTCGGGGGTG GGAAAAGCAG CAAAGGGACA GAAGGACAAA 
GACAAGGATA AGAACAAGGG CAGAGTGAAG GTGGCCCACC AGACCATCGA AGCCCTACAC 
AATAGCGCCC TGGGTATTGC CAACAACGAA GGGTTGTCCG CCCTGCACTT GGCCGCTCAA 
AATGGTCACA ATCAGAGCTC TAGGGAGCTA CTGCTGGCCG GCGCCGATCC CGATGTCCGG 
AATAATTATG GAGATACTCC GCTGCACACC GCCTGCAGAT ATGGACATGC AGGAGTCTCC 
CGGATCCTGC TCTCCGCCCT TTGCGATCCG AATAAAACAA ATCTGAACGG CGACACCGCT 
TTGCACATCA CCTGTGCCAT GGGTCGCAGG AAGCTGACAA GGATTCTCCT AGAGGCAGAT 
GCTCGGCTGG GCATTAAAAA CGCACAAGGC GACTGCCCCA TGCACATAGC GATCAGGAAG 
AACTACCGTG AAATCATCGA GATCCTGAAC ACGCCGAAGA AGATACGGAA TCGCAAGGAG 
AAACCCAAGG AGGGCGGCAG CAGATCGGAC AAGGACAGAG ATCGTGAAAG GGATGTGGTG 
GACAAGGGTA TCAACTGGTC ACCCTATGGC TGTCACTACT TTCCCGATCC GCGTGCCTTT 
CCTTCACCAA AACTGGAGAC ACTGCCCAAG GAGCCACTTA AGTCGGGGGA GCAATACTTC 
CTGGATTTGG CTGGTCATAT TCACAAGGGT CCGGTCAGCG TGGGCAACAC ATGCTATTGC 
GGTCCCTTCT TCCGTCACAT TGAAAACAAA CTAAATTGCA ATCGAAAGAG TTTGAAGAAA 
TACGTTCACA AAACCAAGGA ACGTCTAGGT CACAAAGTTC AGGCATTGGC AATCAAGACG 
AACGATCAGA TTGAACAGCT CACAAGAACG ATGATCGAGG ACAGATTGCG ATGCGAGAGC 
AAGAGGCAGC ACCTCAGTGA GTTCCTGCGA CGAGGTGAAC CTATGCGGTC CACCTTCGAT 
CAGCAGAACA AGAGCTCAAG GATCGAAAGA ACAATGTCCA GGTGCCGCAG TCTAGAGCTC 
TTGGAAAACA ACCACGAAGG TGGTAGGCTC ACCAACTCAC GGAGTGTAGA TGTCCTGGAA 
GATCAGGCAG TGGAGGCTAT AGTACACCGA TCAGCGGAGG TCCAGGTGGA TAGCGACAGC 
GATGACGATT CCAATGAAGC TCGGGATGAA GAAGAGGCAG AAGTGGGTGA CAAGGACCAG 
GTGCAAGAAG AGGCGGAACC AGAGGAGCAC TCGAAGCTAG ATGAACTTAA GCTAGATTTC 
CTAAAAGTCT CCGAAAGACT GGGAGTGCTT TTAGAGAAAA CCACCCTGAT CATGGAAAGG 
GACAGCGAAC AGGAGAGCAA GCGCCAGCTT TCTTCCTTAT CGCCACCTTA TAATCCCCAT 
CCAAGGGCGG AGTCTGCCCA ACTTCGAGAA GATAAAGAGA GTGTTAATAG TCCCAATTTC 
GACGAGTACG CCAACGTGAT ACGACGCTAT CCGAACTCCA GTGAGACCTC AAATCCCTCC 
CAGAACTCCA ACAGCTGGGA CTGGGAGGCA TCACCCACGG GCAGCAATCC CCACCCGGAT 
TACAATAAGT ACTACCAGCG CATTGGGAGA GGAGAGAACA TGCTGAACTC GGTGATCAAG 
GCGCTTCGAA AAGATGCCTC CTTCGCGGAT CTGGGCAAAG AGGAGACGGC TGTCAATGAA 
ACTGCCGAGG GAATGGGAGG CGACAAGCTG CTCTACAAGG AGCAAGCGAG CGAGGCGGCA 
GGTATCTCCA ATCTGATGAA ACGGCAGCCT CTTTGCTTGG AAGACAACTA TCCCGTCATG 
TCGAATTTAT TTTACTCCAA TCCCATCATG GAGTACGCCG AGGAAACAGA AGTGCGTCAA 
AATGGCAATG AAAGTATGAG TAAGGTGGAG ATTCGGAACA GTGAAATCAA GTGCCTAAAG 
AAGCCGAACG CTGGTCAGGT GAGGGATATG GTGGCCCAAC TGCAAAACAC CATTGACTCC 
AATCGACAGG ACTCCCAGAC GGCACATGTG ATCGCCGCAA GATCACACTC CCGCTTGAAC 
AATAACTTCC ATCACTTGGA GGGCACCTCT TCGAACCCTT CGCCCACGTA TAGTATTCCC 
CACCGGAGTA GCGCCCAGTT TGGGCTGCCC GAGAGACACA TACCCAAAGA TGCTTACTTC 
CATGAACTGC CCCACCGTCC ACCGATTCCG CAAACTCCGC AGAGAAGGCT GCACTCCGGC 
TACGTACCAA ATGCGAGTCT CTATCGCAGT GAAGATCTTT ACGTGGGAAG GATTCCACAT 
CCAAATGCTC CACCAAGACA AGTGGACTAC GTGGATGCGG TAGCCTATAG GCCTCTGCAG 
TCCCCCACCT TTCAGGCCAT TGTGCCGCCC CATGTTAATC AAAGGCCAAG GAACTGGCCT 
CATCCCAATC TTCCATCCAA TGAAATCGAT CTGGACGAGC TGTCTGCCGT TGGCCTCTAC 
AACAATGTCT CGAGTCTAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610614.1
CDS	200..2983
Translation	MQHGSSRIPKQEALPNAISGPDSSNWTSLHEAVAAGDDRRVEGLLFSNADRLARESVQGNTPLHEAASRGFSRCVKLICTPPTPPASSGVGKAAKGQKDKDKDKNKGRVKVAHQTIEALHNSALGIANNEGLSALHLAAQNGHNQSSRELLLAGADPDVRNNYGDTPLHTACRYGHAGVSRILLSALCDPNKTNLNGDTALHITCAMGRRKLTRILLEADARLGIKNAQGDCPMHIAIRKNYREIIEILNTPKKIRNRKEKPKEGGSRSDKDRDRERDVVDKGINWSPYGCHYFPDPRAFPSPKLETLPKEPLKSGEQYFLDLAGHIHKGPVSVGNTCYCGPFFRHIENKLNCNRKSLKKYVHKTKERLGHKVQALAIKTNDQIEQLTRTMIEDRLRCESKRQHLSEFLRRGEPMRSTFDQQNKSSRIERTMSRCRSLELLENNHEGGRLTNSRSVDVLEDQAVEAIVHRSAEVQVDSDSDDDSNEARDEEEAEVGDKDQVQEEAEPEEHSKLDELKLDFLKVSERLGVLLEKTTLIMERDSEQESKRQLSSLSPPYNPHPRAESAQLREDKESVNSPNFDEYANVIRRYPNSSETSNPSQNSNSWDWEASPTGSNPHPDYNKYYQRIGRGENMLNSVIKALRKDASFADLGKEETAVNETAEGMGGDKLLYKEQASEAAGISNLMKRQPLCLEDNYPVMSNLFYSNPIMEYAEETEVRQNGNESMSKVEIRNSEIKCLKKPNAGQVRDMVAQLQNTIDSNRQDSQTAHVIAARSHSRLNNNFHHLEGTSSNPSPTYSIPHRSSAQFGLPERHIPKDAYFHELPHRPPIPQTPQRRLHSGYVPNASLYRSEDLYVGRIPHPNAPPRQVDYVDAVAYRPLQSPTFQAIVPPHVNQRPRNWPHPNLPSNEIDLDELSAVGLYNNVSSLV

Target ORF information:

RefSeq Version	NM_136770.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diego (dgo), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136770.2

ORF Insert Sequence:

ATGCAGCATG GATCCTCCCG CATTCCGAAA CAGGAAGCTC TCCCGAATGC GATATCCGGG 
CCCGACTCCA GCAATTGGAC TTCGCTGCAC GAGGCGGTAG CCGCCGGAGA TGATCGCCGG 
GTGGAGGGTC TCCTGTTCAG CAATGCTGAT CGACTGGCCA GGGAATCCGT GCAGGGGAAT 
ACCCCGCTCC ATGAGGCCGC CAGTCGGGGG TTCAGTCGCT GCGTGAAATT GATCTGCACG 
CCCCCCACGC CACCCGCTTC CTCGGGGGTG GGAAAAGCAG CAAAGGGACA GAAGGACAAA 
GACAAGGATA AGAACAAGGG CAGAGTGAAG GTGGCCCACC AGACCATCGA AGCCCTACAC 
AATAGCGCCC TGGGTATTGC CAACAACGAA GGGTTGTCCG CCCTGCACTT GGCCGCTCAA 
AATGGTCACA ATCAGAGCTC TAGGGAGCTA CTGCTGGCCG GCGCCGATCC CGATGTCCGG 
AATAATTATG GAGATACTCC GCTGCACACC GCCTGCAGAT ATGGACATGC AGGAGTCTCC 
CGGATCCTGC TCTCCGCCCT TTGCGATCCG AATAAAACAA ATCTGAACGG CGACACCGCT 
TTGCACATCA CCTGTGCCAT GGGTCGCAGG AAGCTGACAA GGATTCTCCT AGAGGCAGAT 
GCTCGGCTGG GCATTAAAAA CGCACAAGGC GACTGCCCCA TGCACATAGC GATCAGGAAG 
AACTACCGTG AAATCATCGA GATCCTGAAC ACGCCGAAGA AGATACGGAA TCGCAAGGAG 
AAACCCAAGG AGGGCGGCAG CAGATCGGAC AAGGACAGAG ATCGTGAAAG GGATGTGGTG 
GACAAGGGTA TCAACTGGTC ACCCTATGGC TGTCACTACT TTCCCGATCC GCGTGCCTTT 
CCTTCACCAA AACTGGAGAC ACTGCCCAAG GAGCCACTTA AGTCGGGGGA GCAATACTTC 
CTGGATTTGG CTGGTCATAT TCACAAGGGT CCGGTCAGCG TGGGCAACAC ATGCTATTGC 
GGTCCCTTCT TCCGTCACAT TGAAAACAAA CTAAATTGCA ATCGAAAGAG TTTGAAGAAA 
TACGTTCACA AAACCAAGGA ACGTCTAGGT CACAAAGTTC AGGCATTGGC AATCAAGACG 
AACGATCAGA TTGAACAGCT CACAAGAACG ATGATCGAGG ACAGATTGCG ATGCGAGAGC 
AAGAGGCAGC ACCTCAGTGA GTTCCTGCGA CGAGGTGAAC CTATGCGGTC CACCTTCGAT 
CAGCAGAACA AGAGCTCAAG GATCGAAAGA ACAATGTCCA GGTGCCGCAG TCTAGAGCTC 
TTGGAAAACA ACCACGAAGG TGGTAGGCTC ACCAACTCAC GGAGTGTAGA TGTCCTGGAA 
GATCAGGCAG TGGAGGCTAT AGTACACCGA TCAGCGGAGG TCCAGGTGGA TAGCGACAGC 
GATGACGATT CCAATGAAGC TCGGGATGAA GAAGAGGCAG AAGTGGGTGA CAAGGACCAG 
GTGCAAGAAG AGGCGGAACC AGAGGAGCAC TCGAAGCTAG ATGAACTTAA GCTAGATTTC 
CTAAAAGTCT CCGAAAGACT GGGAGTGCTT TTAGAGAAAA CCACCCTGAT CATGGAAAGG 
GACAGCGAAC AGGAGAGCAA GCGCCAGCTT TCTTCCTTAT CGCCACCTTA TAATCCCCAT 
CCAAGGGCGG AGTCTGCCCA ACTTCGAGAA GATAAAGAGA GTGTTAATAG TCCCAATTTC 
GACGAGTACG CCAACGTGAT ACGACGCTAT CCGAACTCCA GTGAGACCTC AAATCCCTCC 
CAGAACTCCA ACAGCTGGGA CTGGGAGGCA TCACCCACGG GCAGCAATCC CCACCCGGAT 
TACAATAAGT ACTACCAGCG CATTGGGAGA GGAGAGAACA TGCTGAACTC GGTGATCAAG 
GCGCTTCGAA AAGATGCCTC CTTCGCGGAT CTGGGCAAAG AGGAGACGGC TGTCAATGAA 
ACTGCCGAGG GAATGGGAGG CGACAAGCTG CTCTACAAGG AGCAAGCGAG CGAGGCGGCA 
GGTATCTCCA ATCTGATGAA ACGGCAGCCT CTTTGCTTGG AAGACAACTA TCCCGTCATG 
TCGAATTTAT TTTACTCCAA TCCCATCATG GAGTACGCCG AGGAAACAGA AGTGCGTCAA 
AATGGCAATG AAAGTATGAG TAAGGTGGAG ATTCGGAACA GTGAAATCAA GTGCCTAAAG 
AAGCCGAACG CTGGTCAGGT GAGGGATATG GTGGCCCAAC TGCAAAACAC CATTGACTCC 
AATCGACAGG ACTCCCAGAC GGCACATGTG ATCGCCGCAA GATCACACTC CCGCTTGAAC 
AATAACTTCC ATCACTTGGA GGGCACCTCT TCGAACCCTT CGCCCACGTA TAGTATTCCC 
CACCGGAGTA GCGCCCAGTT TGGGCTGCCC GAGAGACACA TACCCAAAGA TGCTTACTTC 
CATGAACTGC CCCACCGTCC ACCGATTCCG CAAACTCCGC AGAGAAGGCT GCACTCCGGC 
TACGTACCAA ATGCGAGTCT CTATCGCAGT GAAGATCTTT ACGTGGGAAG GATTCCACAT 
CCAAATGCTC CACCAAGACA AGTGGACTAC GTGGATGCGG TAGCCTATAG GCCTCTGCAG 
TCCCCCACCT TTCAGGCCAT TGTGCCGCCC CATGTTAATC AAAGGCCAAG GAACTGGCCT 
CATCCCAATC TTCCATCCAA TGAAATCGAT CTGGACGAGC TGTCTGCCGT TGGCCTCTAC 
AACAATGTCT CGAGTCTAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgo ( NM_165800.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724973.1 Drosophila melanogaster diego (dgo), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12913
Clone ID Related Accession (Same CDS sequence)	NM_136770.2 , NM_165800.2
Accession Version	NM_165800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diego, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165800.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCATG GATCCTCCCG CATTCCGAAA CAGGAAGCTC TCCCGAATGC GATATCCGGG 
CCCGACTCCA GCAATTGGAC TTCGCTGCAC GAGGCGGTAG CCGCCGGAGA TGATCGCCGG 
GTGGAGGGTC TCCTGTTCAG CAATGCTGAT CGACTGGCCA GGGAATCCGT GCAGGGGAAT 
ACCCCGCTCC ATGAGGCCGC CAGTCGGGGG TTCAGTCGCT GCGTGAAATT GATCTGCACG 
CCCCCCACGC CACCCGCTTC CTCGGGGGTG GGAAAAGCAG CAAAGGGACA GAAGGACAAA 
GACAAGGATA AGAACAAGGG CAGAGTGAAG GTGGCCCACC AGACCATCGA AGCCCTACAC 
AATAGCGCCC TGGGTATTGC CAACAACGAA GGGTTGTCCG CCCTGCACTT GGCCGCTCAA 
AATGGTCACA ATCAGAGCTC TAGGGAGCTA CTGCTGGCCG GCGCCGATCC CGATGTCCGG 
AATAATTATG GAGATACTCC GCTGCACACC GCCTGCAGAT ATGGACATGC AGGAGTCTCC 
CGGATCCTGC TCTCCGCCCT TTGCGATCCG AATAAAACAA ATCTGAACGG CGACACCGCT 
TTGCACATCA CCTGTGCCAT GGGTCGCAGG AAGCTGACAA GGATTCTCCT AGAGGCAGAT 
GCTCGGCTGG GCATTAAAAA CGCACAAGGC GACTGCCCCA TGCACATAGC GATCAGGAAG 
AACTACCGTG AAATCATCGA GATCCTGAAC ACGCCGAAGA AGATACGGAA TCGCAAGGAG 
AAACCCAAGG AGGGCGGCAG CAGATCGGAC AAGGACAGAG ATCGTGAAAG GGATGTGGTG 
GACAAGGGTA TCAACTGGTC ACCCTATGGC TGTCACTACT TTCCCGATCC GCGTGCCTTT 
CCTTCACCAA AACTGGAGAC ACTGCCCAAG GAGCCACTTA AGTCGGGGGA GCAATACTTC 
CTGGATTTGG CTGGTCATAT TCACAAGGGT CCGGTCAGCG TGGGCAACAC ATGCTATTGC 
GGTCCCTTCT TCCGTCACAT TGAAAACAAA CTAAATTGCA ATCGAAAGAG TTTGAAGAAA 
TACGTTCACA AAACCAAGGA ACGTCTAGGT CACAAAGTTC AGGCATTGGC AATCAAGACG 
AACGATCAGA TTGAACAGCT CACAAGAACG ATGATCGAGG ACAGATTGCG ATGCGAGAGC 
AAGAGGCAGC ACCTCAGTGA GTTCCTGCGA CGAGGTGAAC CTATGCGGTC CACCTTCGAT 
CAGCAGAACA AGAGCTCAAG GATCGAAAGA ACAATGTCCA GGTGCCGCAG TCTAGAGCTC 
TTGGAAAACA ACCACGAAGG TGGTAGGCTC ACCAACTCAC GGAGTGTAGA TGTCCTGGAA 
GATCAGGCAG TGGAGGCTAT AGTACACCGA TCAGCGGAGG TCCAGGTGGA TAGCGACAGC 
GATGACGATT CCAATGAAGC TCGGGATGAA GAAGAGGCAG AAGTGGGTGA CAAGGACCAG 
GTGCAAGAAG AGGCGGAACC AGAGGAGCAC TCGAAGCTAG ATGAACTTAA GCTAGATTTC 
CTAAAAGTCT CCGAAAGACT GGGAGTGCTT TTAGAGAAAA CCACCCTGAT CATGGAAAGG 
GACAGCGAAC AGGAGAGCAA GCGCCAGCTT TCTTCCTTAT CGCCACCTTA TAATCCCCAT 
CCAAGGGCGG AGTCTGCCCA ACTTCGAGAA GATAAAGAGA GTGTTAATAG TCCCAATTTC 
GACGAGTACG CCAACGTGAT ACGACGCTAT CCGAACTCCA GTGAGACCTC AAATCCCTCC 
CAGAACTCCA ACAGCTGGGA CTGGGAGGCA TCACCCACGG GCAGCAATCC CCACCCGGAT 
TACAATAAGT ACTACCAGCG CATTGGGAGA GGAGAGAACA TGCTGAACTC GGTGATCAAG 
GCGCTTCGAA AAGATGCCTC CTTCGCGGAT CTGGGCAAAG AGGAGACGGC TGTCAATGAA 
ACTGCCGAGG GAATGGGAGG CGACAAGCTG CTCTACAAGG AGCAAGCGAG CGAGGCGGCA 
GGTATCTCCA ATCTGATGAA ACGGCAGCCT CTTTGCTTGG AAGACAACTA TCCCGTCATG 
TCGAATTTAT TTTACTCCAA TCCCATCATG GAGTACGCCG AGGAAACAGA AGTGCGTCAA 
AATGGCAATG AAAGTATGAG TAAGGTGGAG ATTCGGAACA GTGAAATCAA GTGCCTAAAG 
AAGCCGAACG CTGGTCAGGT GAGGGATATG GTGGCCCAAC TGCAAAACAC CATTGACTCC 
AATCGACAGG ACTCCCAGAC GGCACATGTG ATCGCCGCAA GATCACACTC CCGCTTGAAC 
AATAACTTCC ATCACTTGGA GGGCACCTCT TCGAACCCTT CGCCCACGTA TAGTATTCCC 
CACCGGAGTA GCGCCCAGTT TGGGCTGCCC GAGAGACACA TACCCAAAGA TGCTTACTTC 
CATGAACTGC CCCACCGTCC ACCGATTCCG CAAACTCCGC AGAGAAGGCT GCACTCCGGC 
TACGTACCAA ATGCGAGTCT CTATCGCAGT GAAGATCTTT ACGTGGGAAG GATTCCACAT 
CCAAATGCTC CACCAAGACA AGTGGACTAC GTGGATGCGG TAGCCTATAG GCCTCTGCAG 
TCCCCCACCT TTCAGGCCAT TGTGCCGCCC CATGTTAATC AAAGGCCAAG GAACTGGCCT 
CATCCCAATC TTCCATCCAA TGAAATCGAT CTGGACGAGC TGTCTGCCGT TGGCCTCTAC 
AACAATGTCT CGAGTCTAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724973.1
CDS	597..3380
Translation	MQHGSSRIPKQEALPNAISGPDSSNWTSLHEAVAAGDDRRVEGLLFSNADRLARESVQGNTPLHEAASRGFSRCVKLICTPPTPPASSGVGKAAKGQKDKDKDKNKGRVKVAHQTIEALHNSALGIANNEGLSALHLAAQNGHNQSSRELLLAGADPDVRNNYGDTPLHTACRYGHAGVSRILLSALCDPNKTNLNGDTALHITCAMGRRKLTRILLEADARLGIKNAQGDCPMHIAIRKNYREIIEILNTPKKIRNRKEKPKEGGSRSDKDRDRERDVVDKGINWSPYGCHYFPDPRAFPSPKLETLPKEPLKSGEQYFLDLAGHIHKGPVSVGNTCYCGPFFRHIENKLNCNRKSLKKYVHKTKERLGHKVQALAIKTNDQIEQLTRTMIEDRLRCESKRQHLSEFLRRGEPMRSTFDQQNKSSRIERTMSRCRSLELLENNHEGGRLTNSRSVDVLEDQAVEAIVHRSAEVQVDSDSDDDSNEARDEEEAEVGDKDQVQEEAEPEEHSKLDELKLDFLKVSERLGVLLEKTTLIMERDSEQESKRQLSSLSPPYNPHPRAESAQLREDKESVNSPNFDEYANVIRRYPNSSETSNPSQNSNSWDWEASPTGSNPHPDYNKYYQRIGRGENMLNSVIKALRKDASFADLGKEETAVNETAEGMGGDKLLYKEQASEAAGISNLMKRQPLCLEDNYPVMSNLFYSNPIMEYAEETEVRQNGNESMSKVEIRNSEIKCLKKPNAGQVRDMVAQLQNTIDSNRQDSQTAHVIAARSHSRLNNNFHHLEGTSSNPSPTYSIPHRSSAQFGLPERHIPKDAYFHELPHRPPIPQTPQRRLHSGYVPNASLYRSEDLYVGRIPHPNAPPRQVDYVDAVAYRPLQSPTFQAIVPPHVNQRPRNWPHPNLPSNEIDLDELSAVGLYNNVSSLV

Target ORF information:

RefSeq Version	NM_165800.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diego (dgo), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165800.2

ORF Insert Sequence:

ATGCAGCATG GATCCTCCCG CATTCCGAAA CAGGAAGCTC TCCCGAATGC GATATCCGGG 
CCCGACTCCA GCAATTGGAC TTCGCTGCAC GAGGCGGTAG CCGCCGGAGA TGATCGCCGG 
GTGGAGGGTC TCCTGTTCAG CAATGCTGAT CGACTGGCCA GGGAATCCGT GCAGGGGAAT 
ACCCCGCTCC ATGAGGCCGC CAGTCGGGGG TTCAGTCGCT GCGTGAAATT GATCTGCACG 
CCCCCCACGC CACCCGCTTC CTCGGGGGTG GGAAAAGCAG CAAAGGGACA GAAGGACAAA 
GACAAGGATA AGAACAAGGG CAGAGTGAAG GTGGCCCACC AGACCATCGA AGCCCTACAC 
AATAGCGCCC TGGGTATTGC CAACAACGAA GGGTTGTCCG CCCTGCACTT GGCCGCTCAA 
AATGGTCACA ATCAGAGCTC TAGGGAGCTA CTGCTGGCCG GCGCCGATCC CGATGTCCGG 
AATAATTATG GAGATACTCC GCTGCACACC GCCTGCAGAT ATGGACATGC AGGAGTCTCC 
CGGATCCTGC TCTCCGCCCT TTGCGATCCG AATAAAACAA ATCTGAACGG CGACACCGCT 
TTGCACATCA CCTGTGCCAT GGGTCGCAGG AAGCTGACAA GGATTCTCCT AGAGGCAGAT 
GCTCGGCTGG GCATTAAAAA CGCACAAGGC GACTGCCCCA TGCACATAGC GATCAGGAAG 
AACTACCGTG AAATCATCGA GATCCTGAAC ACGCCGAAGA AGATACGGAA TCGCAAGGAG 
AAACCCAAGG AGGGCGGCAG CAGATCGGAC AAGGACAGAG ATCGTGAAAG GGATGTGGTG 
GACAAGGGTA TCAACTGGTC ACCCTATGGC TGTCACTACT TTCCCGATCC GCGTGCCTTT 
CCTTCACCAA AACTGGAGAC ACTGCCCAAG GAGCCACTTA AGTCGGGGGA GCAATACTTC 
CTGGATTTGG CTGGTCATAT TCACAAGGGT CCGGTCAGCG TGGGCAACAC ATGCTATTGC 
GGTCCCTTCT TCCGTCACAT TGAAAACAAA CTAAATTGCA ATCGAAAGAG TTTGAAGAAA 
TACGTTCACA AAACCAAGGA ACGTCTAGGT CACAAAGTTC AGGCATTGGC AATCAAGACG 
AACGATCAGA TTGAACAGCT CACAAGAACG ATGATCGAGG ACAGATTGCG ATGCGAGAGC 
AAGAGGCAGC ACCTCAGTGA GTTCCTGCGA CGAGGTGAAC CTATGCGGTC CACCTTCGAT 
CAGCAGAACA AGAGCTCAAG GATCGAAAGA ACAATGTCCA GGTGCCGCAG TCTAGAGCTC 
TTGGAAAACA ACCACGAAGG TGGTAGGCTC ACCAACTCAC GGAGTGTAGA TGTCCTGGAA 
GATCAGGCAG TGGAGGCTAT AGTACACCGA TCAGCGGAGG TCCAGGTGGA TAGCGACAGC 
GATGACGATT CCAATGAAGC TCGGGATGAA GAAGAGGCAG AAGTGGGTGA CAAGGACCAG 
GTGCAAGAAG AGGCGGAACC AGAGGAGCAC TCGAAGCTAG ATGAACTTAA GCTAGATTTC 
CTAAAAGTCT CCGAAAGACT GGGAGTGCTT TTAGAGAAAA CCACCCTGAT CATGGAAAGG 
GACAGCGAAC AGGAGAGCAA GCGCCAGCTT TCTTCCTTAT CGCCACCTTA TAATCCCCAT 
CCAAGGGCGG AGTCTGCCCA ACTTCGAGAA GATAAAGAGA GTGTTAATAG TCCCAATTTC 
GACGAGTACG CCAACGTGAT ACGACGCTAT CCGAACTCCA GTGAGACCTC AAATCCCTCC 
CAGAACTCCA ACAGCTGGGA CTGGGAGGCA TCACCCACGG GCAGCAATCC CCACCCGGAT 
TACAATAAGT ACTACCAGCG CATTGGGAGA GGAGAGAACA TGCTGAACTC GGTGATCAAG 
GCGCTTCGAA AAGATGCCTC CTTCGCGGAT CTGGGCAAAG AGGAGACGGC TGTCAATGAA 
ACTGCCGAGG GAATGGGAGG CGACAAGCTG CTCTACAAGG AGCAAGCGAG CGAGGCGGCA 
GGTATCTCCA ATCTGATGAA ACGGCAGCCT CTTTGCTTGG AAGACAACTA TCCCGTCATG 
TCGAATTTAT TTTACTCCAA TCCCATCATG GAGTACGCCG AGGAAACAGA AGTGCGTCAA 
AATGGCAATG AAAGTATGAG TAAGGTGGAG ATTCGGAACA GTGAAATCAA GTGCCTAAAG 
AAGCCGAACG CTGGTCAGGT GAGGGATATG GTGGCCCAAC TGCAAAACAC CATTGACTCC 
AATCGACAGG ACTCCCAGAC GGCACATGTG ATCGCCGCAA GATCACACTC CCGCTTGAAC 
AATAACTTCC ATCACTTGGA GGGCACCTCT TCGAACCCTT CGCCCACGTA TAGTATTCCC 
CACCGGAGTA GCGCCCAGTT TGGGCTGCCC GAGAGACACA TACCCAAAGA TGCTTACTTC 
CATGAACTGC CCCACCGTCC ACCGATTCCG CAAACTCCGC AGAGAAGGCT GCACTCCGGC 
TACGTACCAA ATGCGAGTCT CTATCGCAGT GAAGATCTTT ACGTGGGAAG GATTCCACAT 
CCAAATGCTC CACCAAGACA AGTGGACTAC GTGGATGCGG TAGCCTATAG GCCTCTGCAG 
TCCCCCACCT TTCAGGCCAT TGTGCCGCCC CATGTTAATC AAAGGCCAAG GAACTGGCCT 
CATCCCAATC TTCCATCCAA TGAAATCGAT CTGGACGAGC TGTCTGCCGT TGGCCTCTAC 
AACAATGTCT CGAGTCTAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dgo cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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