Wnt2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wnt2
Entrez Gene ID	35975
Full Name	Wnt oncogene analog 2
Synonyms	CG1916,D-wnt-2,DWnt-2,DWnt2,Dm DWnt2,Dm-2,Dmel\CG1916,Dwnt-2,Dwnt2,Wnt,Wnt-2,dWnt2,wnt-2,wnt2
General protein information	Preferred Names Wnt oncogene analog 2 Names CG1916 gene product from transcript CG1916-RA CG1916-PA CG1916-PB Wnt2-PA Wnt2-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45E1-45E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057462.5	NP_476810.1	Wnt oncogene analog 2, isoform A
NM_001299322.1	NP_001286251.1	Wnt oncogene analog 2, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	WNT7B	XP_538327.2
Homo sapiens (human)	WNT7B	NP_478679.1
Pan troglodytes (chimpanzee)	WNT7B	XP_003317359.2
Rattus norvegicus (Norway rat)	Wnt7b	NP_001009695.1
	WNT7B	XP_001110372.1
Gallus gallus (chicken)	WNT7B	NP_001032351.1
Danio rerio (zebrafish)	wnt7bb	XP_005163010.1
Drosophila melanogaster (fruit fly)	Wnt2	NP_476810.1
Mus musculus (house mouse)	Wnt7b	NP_033554.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010283	XP_001238162.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We discovered an otk paralog (otk2, CG8964), which is co-expressed with otk throughout embryonic and larval development. Otk and Otk2 bind to each other and form complexes with Frizzled, Frizzled2 and Wnt2, pointing to a function as Wnt co-receptors.
Title: The PTK7-related transmembrane proteins off-track and off-track 2 are co-receptors for Drosophila Wnt2 required for male fertility.

Mutations in Wnt2 alter presynaptic motor neuron morphology and presynaptic protein localization at the neuromuscular junction.
Title: Mutations in Wnt2 alter presynaptic motor neuron morphology and presynaptic protein localization at the Drosophila neuromuscular junction.

It activates beta-catenin-independent pathway, such as planar cell polarity pathway, calcium signaling. (review)
Title: [Activation of the beta-catenin-independent pathway by Wnt and the diseases due to its abnormality].

Data show that Drosophila Naked cuticle (Nkd) engages the nuclear import adaptor Importin-alpha3 to antagonize Wnt/beta-catenin signaling.
Title: Drosophila Naked cuticle (Nkd) engages the nuclear import adaptor Importin-alpha3 to antagonize Wnt/beta-catenin signaling.

DWnt-2 promotes correct patterning of direct flight muscles through a mechanism that is independent of the attachment site differentiation initiated by stripe.
Title: Direct flight muscles in Drosophila develop from cells with characteristics of founders and depend on DWnt-2 for their correct patterning.

The following Wnt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wnt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12316	NM_057462.5 Latest version!	Drosophila melanogaster Wnt oncogene analog 2 (Wnt2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21747	NM_001299322.1 Latest version!	Drosophila melanogaster Wnt oncogene analog 2 (Wnt2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wnt2 ( NM_057462.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476810.1 Drosophila melanogaster Wnt oncogene analog 2 (Wnt2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12316
Clone ID Related Accession (Same CDS sequence)	NM_057462.5
Accession Version	NM_057462.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wnt oncogene analog 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057462.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAAAA TACATAACAA GCTCTTAATC TACATACTCT GGATTATGGA AATAAGATTA 
GTGTCCAGCT TTACATCCGT CATGCTCTGT GGCCGGATTC CGGGCCTGAC CCCGGGCCAG 
CGAAATATGT GCCGCGAGAT GCCCGACGCA CTGATTGCCC TGGGCGAGGG CCACCAATTG 
GGCGCCCAGG AGTGCCAGCA CCAGTTCCGA GGACACCGCT GGAACTGCTC GGAGGTGTGG 
CAGCGCAACG TCTTCGCCCA TGTCATACCC ACAGCTTCTC GTGAGGCTGC CTACACCTAT 
GCGATAGCAA GTGCCGGAGC GGCGTACGCG GTGACCGCCG CCTGTGCCCG CGGCAACATC 
TCCACCTGCG GCTGTGATGT GCGGCACAAA GCCACGCCCA CTGGCGGCGG TACGCCCGAC 
GAGCCCTGGA AGTGGGGCGG CTGCTCGGCG GACGTGGACT TTGGGATGCG GTATGCGCGC 
AGATTCATGG ATGCCCGCGA ATTGGAGCGC GACTCCCGCA CGCTGATGAA CCTGCACAAT 
AACCGAGCAG GGAGAACGCT GGTCAAGAAG ATGCTGCGCA CGGACTGCAA GTGCCATGGC 
GTCAGTGGCT CCTGCGTGAT GAAGACCTGC TGGAAGAGCC TGCCGCCCTT TCGACTGGTT 
GGCGATAGAC TCATGCTGAA GTATCAAAAA GCCAAAACTG TTCAAGCCGT CAAAGGCAAA 
CGTGGACTGA GATTAGTATT AAGCAGAAAG AAGCATGCCG GCACCGCTCG TGCCCAGAAA 
CCGGTCCTCG ATTGGCCGAA GCGCATGGAG CTGATCTACC TGGAGGCGTC GCCCAACTAC 
TGCGAGCGAA GTCTGCAGAC GGGCAGCCAG GGCACCTCAG GTCGGACCTG CCAGCGGACC 
GGCCACGGCC CCCAGAGCTG CGATCTGCTC TGCTGTGGGC GTGGCCACAA CACGCAGCAC 
ATTCGAAGGA CCACGCAGTG CCGCTGCCAA TTCCGCTGGT GCTGCGAGGT CAAGTGTGAC 
GAGTGCGACG AGAGCTACGA GGAGTTCACC TGTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476810.1
CDS	1033..2091
Translation	MWKIHNKLLIYILWIMEIRLVSSFTSVMLCGRIPGLTPGQRNMCREMPDALIALGEGHQLGAQECQHQFRGHRWNCSEVWQRNVFAHVIPTASREAAYTYAIASAGAAYAVTAACARGNISTCGCDVRHKATPTGGGTPDEPWKWGGCSADVDFGMRYARRFMDARELERDSRTLMNLHNNRAGRTLVKKMLRTDCKCHGVSGSCVMKTCWKSLPPFRLVGDRLMLKYQKAKTVQAVKGKRGLRLVLSRKKHAGTARAQKPVLDWPKRMELIYLEASPNYCERSLQTGSQGTSGRTCQRTGHGPQSCDLLCCGRGHNTQHIRRTTQCRCQFRWCCEVKCDECDESYEEFTCK

Target ORF information:

RefSeq Version	NM_057462.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wnt oncogene analog 2 (Wnt2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057462.5

ORF Insert Sequence:

ATGTGGAAAA TACATAACAA GCTCTTAATC TACATACTCT GGATTATGGA AATAAGATTA 
GTGTCCAGCT TTACATCCGT CATGCTCTGT GGCCGGATTC CGGGCCTGAC CCCGGGCCAG 
CGAAATATGT GCCGCGAGAT GCCCGACGCA CTGATTGCCC TGGGCGAGGG CCACCAATTG 
GGCGCCCAGG AGTGCCAGCA CCAGTTCCGA GGACACCGCT GGAACTGCTC GGAGGTGTGG 
CAGCGCAACG TCTTCGCCCA TGTCATACCC ACAGCTTCTC GTGAGGCTGC CTACACCTAT 
GCGATAGCAA GTGCCGGAGC GGCGTACGCG GTGACCGCCG CCTGTGCCCG CGGCAACATC 
TCCACCTGCG GCTGTGATGT GCGGCACAAA GCCACGCCCA CTGGCGGCGG TACGCCCGAC 
GAGCCCTGGA AGTGGGGCGG CTGCTCGGCG GACGTGGACT TTGGGATGCG GTATGCGCGC 
AGATTCATGG ATGCCCGCGA ATTGGAGCGC GACTCCCGCA CGCTGATGAA CCTGCACAAT 
AACCGAGCAG GGAGAACGCT GGTCAAGAAG ATGCTGCGCA CGGACTGCAA GTGCCATGGC 
GTCAGTGGCT CCTGCGTGAT GAAGACCTGC TGGAAGAGCC TGCCGCCCTT TCGACTGGTT 
GGCGATAGAC TCATGCTGAA GTATCAAAAA GCCAAAACTG TTCAAGCCGT CAAAGGCAAA 
CGTGGACTGA GATTAGTATT AAGCAGAAAG AAGCATGCCG GCACCGCTCG TGCCCAGAAA 
CCGGTCCTCG ATTGGCCGAA GCGCATGGAG CTGATCTACC TGGAGGCGTC GCCCAACTAC 
TGCGAGCGAA GTCTGCAGAC GGGCAGCCAG GGCACCTCAG GTCGGACCTG CCAGCGGACC 
GGCCACGGCC CCCAGAGCTG CGATCTGCTC TGCTGTGGGC GTGGCCACAA CACGCAGCAC 
ATTCGAAGGA CCACGCAGTG CCGCTGCCAA TTCCGCTGGT GCTGCGAGGT CAAGTGTGAC 
GAGTGCGACG AGAGCTACGA GGAGTTCACC TGTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wnt2 ( NM_001299322.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286251.1 Drosophila melanogaster Wnt oncogene analog 2 (Wnt2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa21747
Clone ID Related Accession (Same CDS sequence)	NM_001299322.1
Accession Version	NM_001299322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wnt oncogene analog 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTGTG GCCGGATTCC GGGCCTGACC CCGGGCCAGC GAAATATGTG CCGCGAGATG 
CCCGACGCAC TGATTGCCCT GGGCGAGGGC CACCAATTGG GCGCCCAGGA GTGCCAGCAC 
CAGTTCCGAG GACACCGCTG GAACTGCTCG GAGGTGTGGC AGCGCAACGT CTTCGCCCAT 
GTCATACCCA CAGCTTCTCG TGAGGCTGCC TACACCTATG CGATAGCAAG TGCCGGAGCG 
GCGTACGCGG TGACCGCCGC CTGTGCCCGC GGCAACATCT CCACCTGCGG CTGTGATGTG 
CGGCACAAAG CCACGCCCAC TGGCGGCGGT ACGCCCGACG AGCCCTGGAA GTGGGGCGGC 
TGCTCGGCGG ACGTGGACTT TGGGATGCGG TATGCGCGCA GATTCATGGA TGCCCGCGAA 
TTGGAGCGCG ACTCCCGCAC GCTGATGAAC CTGCACAATA ACCGAGCAGG GAGAACGCTG 
GTCAAGAAGA TGCTGCGCAC GGACTGCAAG TGCCATGGCG TCAGTGGCTC CTGCGTGATG 
AAGACCTGCT GGAAGAGCCT GCCGCCCTTT CGACTGGTTG GCGATAGACT CATGCTGAAG 
TATCAAAAAG CCAAAACTGT TCAAGCCGTC AAAGGCAAAC GTGGACTGAG ATTAGTATTA 
AGCAGAAAGA AGCATGCCGG CACCGCTCGT GCCCAGAAAC CGGTCCTCGA TTGGCCGAAG 
CGCATGGAGC TGATCTACCT GGAGGCGTCG CCCAACTACT GCGAGCGAAG TCTGCAGACG 
GGCAGCCAGG GCACCTCAGG TCGGACCTGC CAGCGGACCG GCCACGGCCC CCAGAGCTGC 
GATCTGCTCT GCTGTGGGCG TGGCCACAAC ACGCAGCACA TTCGAAGGAC CACGCAGTGC 
CGCTGCCAAT TCCGCTGGTG CTGCGAGGTC AAGTGTGACG AGTGCGACGA GAGCTACGAG 
GAGTTCACCT GTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286251.1
CDS	1109..2086
Translation	MLCGRIPGLTPGQRNMCREMPDALIALGEGHQLGAQECQHQFRGHRWNCSEVWQRNVFAHVIPTASREAAYTYAIASAGAAYAVTAACARGNISTCGCDVRHKATPTGGGTPDEPWKWGGCSADVDFGMRYARRFMDARELERDSRTLMNLHNNRAGRTLVKKMLRTDCKCHGVSGSCVMKTCWKSLPPFRLVGDRLMLKYQKAKTVQAVKGKRGLRLVLSRKKHAGTARAQKPVLDWPKRMELIYLEASPNYCERSLQTGSQGTSGRTCQRTGHGPQSCDLLCCGRGHNTQHIRRTTQCRCQFRWCCEVKCDECDESYEEFTCK

Target ORF information:

RefSeq Version	NM_001299322.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wnt oncogene analog 2 (Wnt2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299322.1

ORF Insert Sequence:

ATGCTCTGTG GCCGGATTCC GGGCCTGACC CCGGGCCAGC GAAATATGTG CCGCGAGATG 
CCCGACGCAC TGATTGCCCT GGGCGAGGGC CACCAATTGG GCGCCCAGGA GTGCCAGCAC 
CAGTTCCGAG GACACCGCTG GAACTGCTCG GAGGTGTGGC AGCGCAACGT CTTCGCCCAT 
GTCATACCCA CAGCTTCTCG TGAGGCTGCC TACACCTATG CGATAGCAAG TGCCGGAGCG 
GCGTACGCGG TGACCGCCGC CTGTGCCCGC GGCAACATCT CCACCTGCGG CTGTGATGTG 
CGGCACAAAG CCACGCCCAC TGGCGGCGGT ACGCCCGACG AGCCCTGGAA GTGGGGCGGC 
TGCTCGGCGG ACGTGGACTT TGGGATGCGG TATGCGCGCA GATTCATGGA TGCCCGCGAA 
TTGGAGCGCG ACTCCCGCAC GCTGATGAAC CTGCACAATA ACCGAGCAGG GAGAACGCTG 
GTCAAGAAGA TGCTGCGCAC GGACTGCAAG TGCCATGGCG TCAGTGGCTC CTGCGTGATG 
AAGACCTGCT GGAAGAGCCT GCCGCCCTTT CGACTGGTTG GCGATAGACT CATGCTGAAG 
TATCAAAAAG CCAAAACTGT TCAAGCCGTC AAAGGCAAAC GTGGACTGAG ATTAGTATTA 
AGCAGAAAGA AGCATGCCGG CACCGCTCGT GCCCAGAAAC CGGTCCTCGA TTGGCCGAAG 
CGCATGGAGC TGATCTACCT GGAGGCGTCG CCCAACTACT GCGAGCGAAG TCTGCAGACG 
GGCAGCCAGG GCACCTCAGG TCGGACCTGC CAGCGGACCG GCCACGGCCC CCAGAGCTGC 
GATCTGCTCT GCTGTGGGCG TGGCCACAAC ACGCAGCACA TTCGAAGGAC CACGCAGTGC 
CGCTGCCAAT TCCGCTGGTG CTGCGAGGTC AAGTGTGACG AGTGCGACGA GAGCTACGAG 
GAGTTCACCT GTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Wnt2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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