Cul1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cul1
Entrez Gene ID	35742
Full Name	Cullin 1
Synonyms	CG1877,CUL1,Cul-1,Cul1[Ex],Cullin-1,Cullin1,Dmel\CG1877,Lin-19,Lin19,Q9V312,cul-1,cul1,dCUL1,dCul-1,dCul1,dmCUL1,l(2)02074,l(2)k01207,lin 19,lin-19/cul-1,lin19,lin19-like
General protein information	Preferred Names Cullin 1 Names CG1877 gene product from transcript CG1877-RC CG1877-PA CG1877-PB CG1877-PC CG1877-PD Cul1-PA Cul1-PB Cul1-PC Cul1-PD cullin cullin 1 cullin-1 cullin1 dCullin dCullin1 lin-19-like lin19-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43F1-43F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_078931.4	NP_523655.1	cullin 1, isoform D
NM_165569.2	NP_724621.1	cullin 1, isoform A
NM_165571.2	NP_724623.1	cullin 1, isoform C
NM_165570.3	NP_724622.1	cullin 1, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cul1	NP_036172.1
Danio rerio (zebrafish)	cul1b	NP_998660.1
Neurospora crassa	NCU05204	XP_962203.2
Xenopus tropicalis (tropical clawed frog)	cul1	NP_001039255.1
	CUL1	XP_002803558.1
Canis lupus familiaris (dog)	CUL1	XP_848402.1
Bos taurus (cattle)	CUL1	NP_001180162.1
Schizosaccharomyces pombe (fission yeast)	cul1	NP_594259.1
Magnaporthe oryzae (rice blast fungus)	MGG_07145	XP_003715342.1
Rattus norvegicus (Norway rat)	Cul1	NP_001102097.1
Gallus gallus (chicken)	CUL1	XP_418878.3
Drosophila melanogaster (fruit fly)	lin19	NP_724622.1
Pan troglodytes (chimpanzee)	CUL1	NP_001233533.1
Caenorhabditis elegans (roundworm)	cul-1	NP_499309.1
Homo sapiens (human)	CUL1	NP_003583.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008007	XP_317457.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutant larvae accumulate high levels of non-neddylated Cul1, suggesting that Int6 is a positive regulator of cullin neddylation
Title: The proto-oncogene Int6 is essential for neddylation of Cul1 and Cul3 in Drosophila.

Distinct protein degradation mechanisms mediated by Cul1 and Cul3 controlling Ci stability in Drosophila eye development.
Title: Distinct protein degradation mechanisms mediated by Cul1 and Cul3 controlling Ci stability in Drosophila eye development.

The following Cul1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cul1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09213	NM_078931.4 Latest version!	Drosophila melanogaster cullin 1, transcript variant D (Cul1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09213	NM_165570.3 Latest version!	Drosophila melanogaster cullin 1, transcript variant B (Cul1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09213	NM_165571.2 Latest version!	Drosophila melanogaster cullin 1, transcript variant C (Cul1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09213	NM_165569.2 Latest version!	Drosophila melanogaster cullin 1, transcript variant A (Cul1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cul1 ( NM_078931.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523655.1 Drosophila melanogaster cullin 1, transcript variant D (Cul1), mRNA.

Price & Availability↑

CloneID	OFa09213
Clone ID Related Accession (Same CDS sequence)	NM_078931.4 , NM_165569.2 , NM_165571.2 , NM_165570.3
Accession Version	NM_078931.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_078931.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCGCT CCGGCAATTC CCAGACCACA CAGAAACTCG TCAACCTGGA CGATATCTGG 
AGCGAATTGG TCGAGGGCAT TATGCAGGTG TTTGAGCACG AGAAGTCCCT CACCCGCAGC 
CAGTACATGC GTTTCTACAC TCACGTCTAT GACTACTGCA CCAGCGTGAG CGCGGCTCCA 
AGCGGACGGA GCAGCGGAAA GACAGGCGGA GCGCAGCTGG TGGGCAAGAA GCTGTACGAC 
CGGCTGGAAC AATTTCTCAA GTCATACCTG AGCGAGCTGC TCACCAAGTT CAAGGCCATC 
AGCGGCGAGG AGGTACTCCT GTCGCGCTAC ACCAAACAGT GGAAGTCGTA TCAGTTCTCC 
AGTACCGTTC TGGACGGCAT CTGCAACTAC CTGAATCGGA ACTGGGTGAA GCGCGAGTGC 
GAGGAGGGGC AGAAGGGTAT CTACAAGATC TACCGACTGG CCCTCGTCGC CTGGAAGGGA 
CATTTGTTCC AAGTGCTAAA TGAGCCGGTC ACCAAGGCAG TGCTCAAGTC CATTGAGGAG 
GAGCGTCAAG GCAAGCTCAT TAACCGGTCG CTGGTCCGCG ACGTTATCGA GTGCTACGTG 
GAGCTCAGCT TTAACGAGGA GGATACCGAT GCCGAGCAGC AGAAGTTATC CGTCTACAAG 
CAAAACTTTG AAAACAAGTT CATCGCAGAC ACATCAGCCT TCTACGAGAA GGAGTCGGAC 
GCCTTTCTGT CCACCAACAC GGTCACCGAG TATCTGAAGC ACGTCGAAAA CAGGCTGGAG 
GAGGAGACGC AGCGCGTCCG CGGCTTTAAT TCAAAGAACG GTCTTTCCTA TCTGCACGAG 
ACCACAGCGG ATGTGCTGAA GTCCACATGC GAAGAGGTTC TTATCGAAAA ACATCTGAAA 
ATCTTTCACA CCGAGTTTCA GAATCTGCTC AACGCAGACC GAAACGATGA TCTGAAACGG 
ATGTACTCCT TGGTCGCCCT CTCGTCCAAA AACCTGACAG ATCTCAAGTC CATCCTTGAG 
AACCATATAC TCCACCAGGG CACGGAGGCC ATTGCCAAGT GTTGCACTAC CGACGCGGCC 
AACGATCCCA AGACGTACGT ACAGACCATC CTGGACGTGC ACAAGAAGTA CAACGCCCTC 
GTTCTGACGG CGTTCAACAA TGACAACGGA TTTGTGGCCG CTTTGGACAA GGCGTGTGGA 
AAGTTCATAA ACTCGAACGT GGTCACGATC GCAAACAGTG CTAGCAAGTC GCCCGAATTG 
CTGGCCAAGT ACTGCGATCT GTTATTAAAA AAATCCTCGA AAAATCCCGA GGACAAGGAG 
CTCGAGGACA ATCTTAACCA GGTGATGGTC GTGTTCAAGT ATATCGAGGA TAAGGACGTC 
TTCCAAAAAT ATTACTCCAA GATGCTTGCT AAGCGCTTGG TTAACCACAC ATCGGCTTCG 
GATGACGCCG AGGCTATGAT GATCTCCAAA CTGAAGCAGA CATGTGGTTA CGAATACACC 
GTCAAGCTTC AGAGGATGTT CCAGGACATT GGCGTCTCAA AGGACTTGAA CTCGTACTTT 
AAGCAGTACC TGGCCGAAAA GAACTTGACC ATGGAAATAG ACTTTGGCAT CGAAGTCCTG 
TCCTCAGGCT CTTGGCCTTT CCAGCTGAGC AACAACTTCC TGCTACCATC TGAGCTGGAG 
CGGTCAGTGC GACAGTTCAA TGAGTTCTAC GCCGCCCGCC ACTCTGGCCG CAAGCTCAAC 
TGGTTGTATC AGATGTGCAA GGGCGAACTG ATAATGAACG TCAACCGCAA TAATTCCTCC 
ACCTACACCC TGCAGGCCAG CACCTTCCAA ATGTCAGTGC TGCTCCAGTT TAACGATCAG 
CTCAGTTTTA CAGTCCAGCA ACTGCAGGAC AACACACAGA CGCAGCAGGA GAACTTGATT 
CAGGTATTGC AAATACTGCT CAAGGCGAAG GTGCTGACCT CCAGCGACAA TGAGAACTCG 
CTGACGCCAG AGTCGACGGT GGAGCTGTTC CTCGATTACA AAAACAAGAA GCGCCGAATT 
AACATCAACC AGCCTTTGAA GACTGAACTT AAAGTGGAGC AGGAGACTGT ACATAAGCAC 
ATAGAGGAGG ACCGAAAATT ACTGATCCAG GCGGCGATCG TCCGCATCAT GAAGATGCGC 
AAACGACTCA ACCACACCAA TCTCATATCG GAGGTGCTTA ATCAGCTGTC AACGCGCTTT 
AAGCCTAAGG TGCCCGTGAT CAAGAAGTGC ATAGACATCC TGATCGAGAA GGAGTATCTA 
GAGCGAATGG AGGGACACAA AGACACATAT AGTTATCTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523655.1
CDS	367..2691
Translation	MNRSGNSQTTQKLVNLDDIWSELVEGIMQVFEHEKSLTRSQYMRFYTHVYDYCTSVSAAPSGRSSGKTGGAQLVGKKLYDRLEQFLKSYLSELLTKFKAISGEEVLLSRYTKQWKSYQFSSTVLDGICNYLNRNWVKRECEEGQKGIYKIYRLALVAWKGHLFQVLNEPVTKAVLKSIEEERQGKLINRSLVRDVIECYVELSFNEEDTDAEQQKLSVYKQNFENKFIADTSAFYEKESDAFLSTNTVTEYLKHVENRLEEETQRVRGFNSKNGLSYLHETTADVLKSTCEEVLIEKHLKIFHTEFQNLLNADRNDDLKRMYSLVALSSKNLTDLKSILENHILHQGTEAIAKCCTTDAANDPKTYVQTILDVHKKYNALVLTAFNNDNGFVAALDKACGKFINSNVVTIANSASKSPELLAKYCDLLLKKSSKNPEDKELEDNLNQVMVVFKYIEDKDVFQKYYSKMLAKRLVNHTSASDDAEAMMISKLKQTCGYEYTVKLQRMFQDIGVSKDLNSYFKQYLAEKNLTMEIDFGIEVLSSGSWPFQLSNNFLLPSELERSVRQFNEFYAARHSGRKLNWLYQMCKGELIMNVNRNNSSTYTLQASTFQMSVLLQFNDQLSFTVQQLQDNTQTQQENLIQVLQILLKAKVLTSSDNENSLTPESTVELFLDYKNKKRRININQPLKTELKVEQETVHKHIEEDRKLLIQAAIVRIMKMRKRLNHTNLISEVLNQLSTRFKPKVPVIKKCIDILIEKEYLERMEGHKDTYSYLA

Target ORF information:

RefSeq Version	NM_078931.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 1, transcript variant D (Cul1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078931.4

ORF Insert Sequence:

ATGAACCGCT CCGGCAATTC CCAGACCACA CAGAAACTCG TCAACCTGGA CGATATCTGG 
AGCGAATTGG TCGAGGGCAT TATGCAGGTG TTTGAGCACG AGAAGTCCCT CACCCGCAGC 
CAGTACATGC GTTTCTACAC TCACGTCTAT GACTACTGCA CCAGCGTGAG CGCGGCTCCA 
AGCGGACGGA GCAGCGGAAA GACAGGCGGA GCGCAGCTGG TGGGCAAGAA GCTGTACGAC 
CGGCTGGAAC AATTTCTCAA GTCATACCTG AGCGAGCTGC TCACCAAGTT CAAGGCCATC 
AGCGGCGAGG AGGTACTCCT GTCGCGCTAC ACCAAACAGT GGAAGTCGTA TCAGTTCTCC 
AGTACCGTTC TGGACGGCAT CTGCAACTAC CTGAATCGGA ACTGGGTGAA GCGCGAGTGC 
GAGGAGGGGC AGAAGGGTAT CTACAAGATC TACCGACTGG CCCTCGTCGC CTGGAAGGGA 
CATTTGTTCC AAGTGCTAAA TGAGCCGGTC ACCAAGGCAG TGCTCAAGTC CATTGAGGAG 
GAGCGTCAAG GCAAGCTCAT TAACCGGTCG CTGGTCCGCG ACGTTATCGA GTGCTACGTG 
GAGCTCAGCT TTAACGAGGA GGATACCGAT GCCGAGCAGC AGAAGTTATC CGTCTACAAG 
CAAAACTTTG AAAACAAGTT CATCGCAGAC ACATCAGCCT TCTACGAGAA GGAGTCGGAC 
GCCTTTCTGT CCACCAACAC GGTCACCGAG TATCTGAAGC ACGTCGAAAA CAGGCTGGAG 
GAGGAGACGC AGCGCGTCCG CGGCTTTAAT TCAAAGAACG GTCTTTCCTA TCTGCACGAG 
ACCACAGCGG ATGTGCTGAA GTCCACATGC GAAGAGGTTC TTATCGAAAA ACATCTGAAA 
ATCTTTCACA CCGAGTTTCA GAATCTGCTC AACGCAGACC GAAACGATGA TCTGAAACGG 
ATGTACTCCT TGGTCGCCCT CTCGTCCAAA AACCTGACAG ATCTCAAGTC CATCCTTGAG 
AACCATATAC TCCACCAGGG CACGGAGGCC ATTGCCAAGT GTTGCACTAC CGACGCGGCC 
AACGATCCCA AGACGTACGT ACAGACCATC CTGGACGTGC ACAAGAAGTA CAACGCCCTC 
GTTCTGACGG CGTTCAACAA TGACAACGGA TTTGTGGCCG CTTTGGACAA GGCGTGTGGA 
AAGTTCATAA ACTCGAACGT GGTCACGATC GCAAACAGTG CTAGCAAGTC GCCCGAATTG 
CTGGCCAAGT ACTGCGATCT GTTATTAAAA AAATCCTCGA AAAATCCCGA GGACAAGGAG 
CTCGAGGACA ATCTTAACCA GGTGATGGTC GTGTTCAAGT ATATCGAGGA TAAGGACGTC 
TTCCAAAAAT ATTACTCCAA GATGCTTGCT AAGCGCTTGG TTAACCACAC ATCGGCTTCG 
GATGACGCCG AGGCTATGAT GATCTCCAAA CTGAAGCAGA CATGTGGTTA CGAATACACC 
GTCAAGCTTC AGAGGATGTT CCAGGACATT GGCGTCTCAA AGGACTTGAA CTCGTACTTT 
AAGCAGTACC TGGCCGAAAA GAACTTGACC ATGGAAATAG ACTTTGGCAT CGAAGTCCTG 
TCCTCAGGCT CTTGGCCTTT CCAGCTGAGC AACAACTTCC TGCTACCATC TGAGCTGGAG 
CGGTCAGTGC GACAGTTCAA TGAGTTCTAC GCCGCCCGCC ACTCTGGCCG CAAGCTCAAC 
TGGTTGTATC AGATGTGCAA GGGCGAACTG ATAATGAACG TCAACCGCAA TAATTCCTCC 
ACCTACACCC TGCAGGCCAG CACCTTCCAA ATGTCAGTGC TGCTCCAGTT TAACGATCAG 
CTCAGTTTTA CAGTCCAGCA ACTGCAGGAC AACACACAGA CGCAGCAGGA GAACTTGATT 
CAGGTATTGC AAATACTGCT CAAGGCGAAG GTGCTGACCT CCAGCGACAA TGAGAACTCG 
CTGACGCCAG AGTCGACGGT GGAGCTGTTC CTCGATTACA AAAACAAGAA GCGCCGAATT 
AACATCAACC AGCCTTTGAA GACTGAACTT AAAGTGGAGC AGGAGACTGT ACATAAGCAC 
ATAGAGGAGG ACCGAAAATT ACTGATCCAG GCGGCGATCG TCCGCATCAT GAAGATGCGC 
AAACGACTCA ACCACACCAA TCTCATATCG GAGGTGCTTA ATCAGCTGTC AACGCGCTTT 
AAGCCTAAGG TGCCCGTGAT CAAGAAGTGC ATAGACATCC TGATCGAGAA GGAGTATCTA 
GAGCGAATGG AGGGACACAA AGACACATAT AGTTATCTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul1 ( NM_165569.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724621.1 Drosophila melanogaster cullin 1, transcript variant A (Cul1), mRNA.

Price & Availability↑

CloneID	OFa09213
Clone ID Related Accession (Same CDS sequence)	NM_078931.4 , NM_165569.2 , NM_165571.2 , NM_165570.3
Accession Version	NM_165569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_165569.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCGCT CCGGCAATTC CCAGACCACA CAGAAACTCG TCAACCTGGA CGATATCTGG 
AGCGAATTGG TCGAGGGCAT TATGCAGGTG TTTGAGCACG AGAAGTCCCT CACCCGCAGC 
CAGTACATGC GTTTCTACAC TCACGTCTAT GACTACTGCA CCAGCGTGAG CGCGGCTCCA 
AGCGGACGGA GCAGCGGAAA GACAGGCGGA GCGCAGCTGG TGGGCAAGAA GCTGTACGAC 
CGGCTGGAAC AATTTCTCAA GTCATACCTG AGCGAGCTGC TCACCAAGTT CAAGGCCATC 
AGCGGCGAGG AGGTACTCCT GTCGCGCTAC ACCAAACAGT GGAAGTCGTA TCAGTTCTCC 
AGTACCGTTC TGGACGGCAT CTGCAACTAC CTGAATCGGA ACTGGGTGAA GCGCGAGTGC 
GAGGAGGGGC AGAAGGGTAT CTACAAGATC TACCGACTGG CCCTCGTCGC CTGGAAGGGA 
CATTTGTTCC AAGTGCTAAA TGAGCCGGTC ACCAAGGCAG TGCTCAAGTC CATTGAGGAG 
GAGCGTCAAG GCAAGCTCAT TAACCGGTCG CTGGTCCGCG ACGTTATCGA GTGCTACGTG 
GAGCTCAGCT TTAACGAGGA GGATACCGAT GCCGAGCAGC AGAAGTTATC CGTCTACAAG 
CAAAACTTTG AAAACAAGTT CATCGCAGAC ACATCAGCCT TCTACGAGAA GGAGTCGGAC 
GCCTTTCTGT CCACCAACAC GGTCACCGAG TATCTGAAGC ACGTCGAAAA CAGGCTGGAG 
GAGGAGACGC AGCGCGTCCG CGGCTTTAAT TCAAAGAACG GTCTTTCCTA TCTGCACGAG 
ACCACAGCGG ATGTGCTGAA GTCCACATGC GAAGAGGTTC TTATCGAAAA ACATCTGAAA 
ATCTTTCACA CCGAGTTTCA GAATCTGCTC AACGCAGACC GAAACGATGA TCTGAAACGG 
ATGTACTCCT TGGTCGCCCT CTCGTCCAAA AACCTGACAG ATCTCAAGTC CATCCTTGAG 
AACCATATAC TCCACCAGGG CACGGAGGCC ATTGCCAAGT GTTGCACTAC CGACGCGGCC 
AACGATCCCA AGACGTACGT ACAGACCATC CTGGACGTGC ACAAGAAGTA CAACGCCCTC 
GTTCTGACGG CGTTCAACAA TGACAACGGA TTTGTGGCCG CTTTGGACAA GGCGTGTGGA 
AAGTTCATAA ACTCGAACGT GGTCACGATC GCAAACAGTG CTAGCAAGTC GCCCGAATTG 
CTGGCCAAGT ACTGCGATCT GTTATTAAAA AAATCCTCGA AAAATCCCGA GGACAAGGAG 
CTCGAGGACA ATCTTAACCA GGTGATGGTC GTGTTCAAGT ATATCGAGGA TAAGGACGTC 
TTCCAAAAAT ATTACTCCAA GATGCTTGCT AAGCGCTTGG TTAACCACAC ATCGGCTTCG 
GATGACGCCG AGGCTATGAT GATCTCCAAA CTGAAGCAGA CATGTGGTTA CGAATACACC 
GTCAAGCTTC AGAGGATGTT CCAGGACATT GGCGTCTCAA AGGACTTGAA CTCGTACTTT 
AAGCAGTACC TGGCCGAAAA GAACTTGACC ATGGAAATAG ACTTTGGCAT CGAAGTCCTG 
TCCTCAGGCT CTTGGCCTTT CCAGCTGAGC AACAACTTCC TGCTACCATC TGAGCTGGAG 
CGGTCAGTGC GACAGTTCAA TGAGTTCTAC GCCGCCCGCC ACTCTGGCCG CAAGCTCAAC 
TGGTTGTATC AGATGTGCAA GGGCGAACTG ATAATGAACG TCAACCGCAA TAATTCCTCC 
ACCTACACCC TGCAGGCCAG CACCTTCCAA ATGTCAGTGC TGCTCCAGTT TAACGATCAG 
CTCAGTTTTA CAGTCCAGCA ACTGCAGGAC AACACACAGA CGCAGCAGGA GAACTTGATT 
CAGGTATTGC AAATACTGCT CAAGGCGAAG GTGCTGACCT CCAGCGACAA TGAGAACTCG 
CTGACGCCAG AGTCGACGGT GGAGCTGTTC CTCGATTACA AAAACAAGAA GCGCCGAATT 
AACATCAACC AGCCTTTGAA GACTGAACTT AAAGTGGAGC AGGAGACTGT ACATAAGCAC 
ATAGAGGAGG ACCGAAAATT ACTGATCCAG GCGGCGATCG TCCGCATCAT GAAGATGCGC 
AAACGACTCA ACCACACCAA TCTCATATCG GAGGTGCTTA ATCAGCTGTC AACGCGCTTT 
AAGCCTAAGG TGCCCGTGAT CAAGAAGTGC ATAGACATCC TGATCGAGAA GGAGTATCTA 
GAGCGAATGG AGGGACACAA AGACACATAT AGTTATCTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724621.1
CDS	390..2714
Translation	MNRSGNSQTTQKLVNLDDIWSELVEGIMQVFEHEKSLTRSQYMRFYTHVYDYCTSVSAAPSGRSSGKTGGAQLVGKKLYDRLEQFLKSYLSELLTKFKAISGEEVLLSRYTKQWKSYQFSSTVLDGICNYLNRNWVKRECEEGQKGIYKIYRLALVAWKGHLFQVLNEPVTKAVLKSIEEERQGKLINRSLVRDVIECYVELSFNEEDTDAEQQKLSVYKQNFENKFIADTSAFYEKESDAFLSTNTVTEYLKHVENRLEEETQRVRGFNSKNGLSYLHETTADVLKSTCEEVLIEKHLKIFHTEFQNLLNADRNDDLKRMYSLVALSSKNLTDLKSILENHILHQGTEAIAKCCTTDAANDPKTYVQTILDVHKKYNALVLTAFNNDNGFVAALDKACGKFINSNVVTIANSASKSPELLAKYCDLLLKKSSKNPEDKELEDNLNQVMVVFKYIEDKDVFQKYYSKMLAKRLVNHTSASDDAEAMMISKLKQTCGYEYTVKLQRMFQDIGVSKDLNSYFKQYLAEKNLTMEIDFGIEVLSSGSWPFQLSNNFLLPSELERSVRQFNEFYAARHSGRKLNWLYQMCKGELIMNVNRNNSSTYTLQASTFQMSVLLQFNDQLSFTVQQLQDNTQTQQENLIQVLQILLKAKVLTSSDNENSLTPESTVELFLDYKNKKRRININQPLKTELKVEQETVHKHIEEDRKLLIQAAIVRIMKMRKRLNHTNLISEVLNQLSTRFKPKVPVIKKCIDILIEKEYLERMEGHKDTYSYLA

Target ORF information:

RefSeq Version	NM_165569.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 1, transcript variant A (Cul1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165569.2

ORF Insert Sequence:

ATGAACCGCT CCGGCAATTC CCAGACCACA CAGAAACTCG TCAACCTGGA CGATATCTGG 
AGCGAATTGG TCGAGGGCAT TATGCAGGTG TTTGAGCACG AGAAGTCCCT CACCCGCAGC 
CAGTACATGC GTTTCTACAC TCACGTCTAT GACTACTGCA CCAGCGTGAG CGCGGCTCCA 
AGCGGACGGA GCAGCGGAAA GACAGGCGGA GCGCAGCTGG TGGGCAAGAA GCTGTACGAC 
CGGCTGGAAC AATTTCTCAA GTCATACCTG AGCGAGCTGC TCACCAAGTT CAAGGCCATC 
AGCGGCGAGG AGGTACTCCT GTCGCGCTAC ACCAAACAGT GGAAGTCGTA TCAGTTCTCC 
AGTACCGTTC TGGACGGCAT CTGCAACTAC CTGAATCGGA ACTGGGTGAA GCGCGAGTGC 
GAGGAGGGGC AGAAGGGTAT CTACAAGATC TACCGACTGG CCCTCGTCGC CTGGAAGGGA 
CATTTGTTCC AAGTGCTAAA TGAGCCGGTC ACCAAGGCAG TGCTCAAGTC CATTGAGGAG 
GAGCGTCAAG GCAAGCTCAT TAACCGGTCG CTGGTCCGCG ACGTTATCGA GTGCTACGTG 
GAGCTCAGCT TTAACGAGGA GGATACCGAT GCCGAGCAGC AGAAGTTATC CGTCTACAAG 
CAAAACTTTG AAAACAAGTT CATCGCAGAC ACATCAGCCT TCTACGAGAA GGAGTCGGAC 
GCCTTTCTGT CCACCAACAC GGTCACCGAG TATCTGAAGC ACGTCGAAAA CAGGCTGGAG 
GAGGAGACGC AGCGCGTCCG CGGCTTTAAT TCAAAGAACG GTCTTTCCTA TCTGCACGAG 
ACCACAGCGG ATGTGCTGAA GTCCACATGC GAAGAGGTTC TTATCGAAAA ACATCTGAAA 
ATCTTTCACA CCGAGTTTCA GAATCTGCTC AACGCAGACC GAAACGATGA TCTGAAACGG 
ATGTACTCCT TGGTCGCCCT CTCGTCCAAA AACCTGACAG ATCTCAAGTC CATCCTTGAG 
AACCATATAC TCCACCAGGG CACGGAGGCC ATTGCCAAGT GTTGCACTAC CGACGCGGCC 
AACGATCCCA AGACGTACGT ACAGACCATC CTGGACGTGC ACAAGAAGTA CAACGCCCTC 
GTTCTGACGG CGTTCAACAA TGACAACGGA TTTGTGGCCG CTTTGGACAA GGCGTGTGGA 
AAGTTCATAA ACTCGAACGT GGTCACGATC GCAAACAGTG CTAGCAAGTC GCCCGAATTG 
CTGGCCAAGT ACTGCGATCT GTTATTAAAA AAATCCTCGA AAAATCCCGA GGACAAGGAG 
CTCGAGGACA ATCTTAACCA GGTGATGGTC GTGTTCAAGT ATATCGAGGA TAAGGACGTC 
TTCCAAAAAT ATTACTCCAA GATGCTTGCT AAGCGCTTGG TTAACCACAC ATCGGCTTCG 
GATGACGCCG AGGCTATGAT GATCTCCAAA CTGAAGCAGA CATGTGGTTA CGAATACACC 
GTCAAGCTTC AGAGGATGTT CCAGGACATT GGCGTCTCAA AGGACTTGAA CTCGTACTTT 
AAGCAGTACC TGGCCGAAAA GAACTTGACC ATGGAAATAG ACTTTGGCAT CGAAGTCCTG 
TCCTCAGGCT CTTGGCCTTT CCAGCTGAGC AACAACTTCC TGCTACCATC TGAGCTGGAG 
CGGTCAGTGC GACAGTTCAA TGAGTTCTAC GCCGCCCGCC ACTCTGGCCG CAAGCTCAAC 
TGGTTGTATC AGATGTGCAA GGGCGAACTG ATAATGAACG TCAACCGCAA TAATTCCTCC 
ACCTACACCC TGCAGGCCAG CACCTTCCAA ATGTCAGTGC TGCTCCAGTT TAACGATCAG 
CTCAGTTTTA CAGTCCAGCA ACTGCAGGAC AACACACAGA CGCAGCAGGA GAACTTGATT 
CAGGTATTGC AAATACTGCT CAAGGCGAAG GTGCTGACCT CCAGCGACAA TGAGAACTCG 
CTGACGCCAG AGTCGACGGT GGAGCTGTTC CTCGATTACA AAAACAAGAA GCGCCGAATT 
AACATCAACC AGCCTTTGAA GACTGAACTT AAAGTGGAGC AGGAGACTGT ACATAAGCAC 
ATAGAGGAGG ACCGAAAATT ACTGATCCAG GCGGCGATCG TCCGCATCAT GAAGATGCGC 
AAACGACTCA ACCACACCAA TCTCATATCG GAGGTGCTTA ATCAGCTGTC AACGCGCTTT 
AAGCCTAAGG TGCCCGTGAT CAAGAAGTGC ATAGACATCC TGATCGAGAA GGAGTATCTA 
GAGCGAATGG AGGGACACAA AGACACATAT AGTTATCTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul1 ( NM_165571.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724623.1 Drosophila melanogaster cullin 1, transcript variant C (Cul1), mRNA.

Price & Availability↑

CloneID	OFa09213
Clone ID Related Accession (Same CDS sequence)	NM_078931.4 , NM_165569.2 , NM_165571.2 , NM_165570.3
Accession Version	NM_165571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_165571.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCGCT CCGGCAATTC CCAGACCACA CAGAAACTCG TCAACCTGGA CGATATCTGG 
AGCGAATTGG TCGAGGGCAT TATGCAGGTG TTTGAGCACG AGAAGTCCCT CACCCGCAGC 
CAGTACATGC GTTTCTACAC TCACGTCTAT GACTACTGCA CCAGCGTGAG CGCGGCTCCA 
AGCGGACGGA GCAGCGGAAA GACAGGCGGA GCGCAGCTGG TGGGCAAGAA GCTGTACGAC 
CGGCTGGAAC AATTTCTCAA GTCATACCTG AGCGAGCTGC TCACCAAGTT CAAGGCCATC 
AGCGGCGAGG AGGTACTCCT GTCGCGCTAC ACCAAACAGT GGAAGTCGTA TCAGTTCTCC 
AGTACCGTTC TGGACGGCAT CTGCAACTAC CTGAATCGGA ACTGGGTGAA GCGCGAGTGC 
GAGGAGGGGC AGAAGGGTAT CTACAAGATC TACCGACTGG CCCTCGTCGC CTGGAAGGGA 
CATTTGTTCC AAGTGCTAAA TGAGCCGGTC ACCAAGGCAG TGCTCAAGTC CATTGAGGAG 
GAGCGTCAAG GCAAGCTCAT TAACCGGTCG CTGGTCCGCG ACGTTATCGA GTGCTACGTG 
GAGCTCAGCT TTAACGAGGA GGATACCGAT GCCGAGCAGC AGAAGTTATC CGTCTACAAG 
CAAAACTTTG AAAACAAGTT CATCGCAGAC ACATCAGCCT TCTACGAGAA GGAGTCGGAC 
GCCTTTCTGT CCACCAACAC GGTCACCGAG TATCTGAAGC ACGTCGAAAA CAGGCTGGAG 
GAGGAGACGC AGCGCGTCCG CGGCTTTAAT TCAAAGAACG GTCTTTCCTA TCTGCACGAG 
ACCACAGCGG ATGTGCTGAA GTCCACATGC GAAGAGGTTC TTATCGAAAA ACATCTGAAA 
ATCTTTCACA CCGAGTTTCA GAATCTGCTC AACGCAGACC GAAACGATGA TCTGAAACGG 
ATGTACTCCT TGGTCGCCCT CTCGTCCAAA AACCTGACAG ATCTCAAGTC CATCCTTGAG 
AACCATATAC TCCACCAGGG CACGGAGGCC ATTGCCAAGT GTTGCACTAC CGACGCGGCC 
AACGATCCCA AGACGTACGT ACAGACCATC CTGGACGTGC ACAAGAAGTA CAACGCCCTC 
GTTCTGACGG CGTTCAACAA TGACAACGGA TTTGTGGCCG CTTTGGACAA GGCGTGTGGA 
AAGTTCATAA ACTCGAACGT GGTCACGATC GCAAACAGTG CTAGCAAGTC GCCCGAATTG 
CTGGCCAAGT ACTGCGATCT GTTATTAAAA AAATCCTCGA AAAATCCCGA GGACAAGGAG 
CTCGAGGACA ATCTTAACCA GGTGATGGTC GTGTTCAAGT ATATCGAGGA TAAGGACGTC 
TTCCAAAAAT ATTACTCCAA GATGCTTGCT AAGCGCTTGG TTAACCACAC ATCGGCTTCG 
GATGACGCCG AGGCTATGAT GATCTCCAAA CTGAAGCAGA CATGTGGTTA CGAATACACC 
GTCAAGCTTC AGAGGATGTT CCAGGACATT GGCGTCTCAA AGGACTTGAA CTCGTACTTT 
AAGCAGTACC TGGCCGAAAA GAACTTGACC ATGGAAATAG ACTTTGGCAT CGAAGTCCTG 
TCCTCAGGCT CTTGGCCTTT CCAGCTGAGC AACAACTTCC TGCTACCATC TGAGCTGGAG 
CGGTCAGTGC GACAGTTCAA TGAGTTCTAC GCCGCCCGCC ACTCTGGCCG CAAGCTCAAC 
TGGTTGTATC AGATGTGCAA GGGCGAACTG ATAATGAACG TCAACCGCAA TAATTCCTCC 
ACCTACACCC TGCAGGCCAG CACCTTCCAA ATGTCAGTGC TGCTCCAGTT TAACGATCAG 
CTCAGTTTTA CAGTCCAGCA ACTGCAGGAC AACACACAGA CGCAGCAGGA GAACTTGATT 
CAGGTATTGC AAATACTGCT CAAGGCGAAG GTGCTGACCT CCAGCGACAA TGAGAACTCG 
CTGACGCCAG AGTCGACGGT GGAGCTGTTC CTCGATTACA AAAACAAGAA GCGCCGAATT 
AACATCAACC AGCCTTTGAA GACTGAACTT AAAGTGGAGC AGGAGACTGT ACATAAGCAC 
ATAGAGGAGG ACCGAAAATT ACTGATCCAG GCGGCGATCG TCCGCATCAT GAAGATGCGC 
AAACGACTCA ACCACACCAA TCTCATATCG GAGGTGCTTA ATCAGCTGTC AACGCGCTTT 
AAGCCTAAGG TGCCCGTGAT CAAGAAGTGC ATAGACATCC TGATCGAGAA GGAGTATCTA 
GAGCGAATGG AGGGACACAA AGACACATAT AGTTATCTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724623.1
CDS	408..2732
Translation	MNRSGNSQTTQKLVNLDDIWSELVEGIMQVFEHEKSLTRSQYMRFYTHVYDYCTSVSAAPSGRSSGKTGGAQLVGKKLYDRLEQFLKSYLSELLTKFKAISGEEVLLSRYTKQWKSYQFSSTVLDGICNYLNRNWVKRECEEGQKGIYKIYRLALVAWKGHLFQVLNEPVTKAVLKSIEEERQGKLINRSLVRDVIECYVELSFNEEDTDAEQQKLSVYKQNFENKFIADTSAFYEKESDAFLSTNTVTEYLKHVENRLEEETQRVRGFNSKNGLSYLHETTADVLKSTCEEVLIEKHLKIFHTEFQNLLNADRNDDLKRMYSLVALSSKNLTDLKSILENHILHQGTEAIAKCCTTDAANDPKTYVQTILDVHKKYNALVLTAFNNDNGFVAALDKACGKFINSNVVTIANSASKSPELLAKYCDLLLKKSSKNPEDKELEDNLNQVMVVFKYIEDKDVFQKYYSKMLAKRLVNHTSASDDAEAMMISKLKQTCGYEYTVKLQRMFQDIGVSKDLNSYFKQYLAEKNLTMEIDFGIEVLSSGSWPFQLSNNFLLPSELERSVRQFNEFYAARHSGRKLNWLYQMCKGELIMNVNRNNSSTYTLQASTFQMSVLLQFNDQLSFTVQQLQDNTQTQQENLIQVLQILLKAKVLTSSDNENSLTPESTVELFLDYKNKKRRININQPLKTELKVEQETVHKHIEEDRKLLIQAAIVRIMKMRKRLNHTNLISEVLNQLSTRFKPKVPVIKKCIDILIEKEYLERMEGHKDTYSYLA

Target ORF information:

RefSeq Version	NM_165571.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 1, transcript variant C (Cul1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165571.2

ORF Insert Sequence:

ATGAACCGCT CCGGCAATTC CCAGACCACA CAGAAACTCG TCAACCTGGA CGATATCTGG 
AGCGAATTGG TCGAGGGCAT TATGCAGGTG TTTGAGCACG AGAAGTCCCT CACCCGCAGC 
CAGTACATGC GTTTCTACAC TCACGTCTAT GACTACTGCA CCAGCGTGAG CGCGGCTCCA 
AGCGGACGGA GCAGCGGAAA GACAGGCGGA GCGCAGCTGG TGGGCAAGAA GCTGTACGAC 
CGGCTGGAAC AATTTCTCAA GTCATACCTG AGCGAGCTGC TCACCAAGTT CAAGGCCATC 
AGCGGCGAGG AGGTACTCCT GTCGCGCTAC ACCAAACAGT GGAAGTCGTA TCAGTTCTCC 
AGTACCGTTC TGGACGGCAT CTGCAACTAC CTGAATCGGA ACTGGGTGAA GCGCGAGTGC 
GAGGAGGGGC AGAAGGGTAT CTACAAGATC TACCGACTGG CCCTCGTCGC CTGGAAGGGA 
CATTTGTTCC AAGTGCTAAA TGAGCCGGTC ACCAAGGCAG TGCTCAAGTC CATTGAGGAG 
GAGCGTCAAG GCAAGCTCAT TAACCGGTCG CTGGTCCGCG ACGTTATCGA GTGCTACGTG 
GAGCTCAGCT TTAACGAGGA GGATACCGAT GCCGAGCAGC AGAAGTTATC CGTCTACAAG 
CAAAACTTTG AAAACAAGTT CATCGCAGAC ACATCAGCCT TCTACGAGAA GGAGTCGGAC 
GCCTTTCTGT CCACCAACAC GGTCACCGAG TATCTGAAGC ACGTCGAAAA CAGGCTGGAG 
GAGGAGACGC AGCGCGTCCG CGGCTTTAAT TCAAAGAACG GTCTTTCCTA TCTGCACGAG 
ACCACAGCGG ATGTGCTGAA GTCCACATGC GAAGAGGTTC TTATCGAAAA ACATCTGAAA 
ATCTTTCACA CCGAGTTTCA GAATCTGCTC AACGCAGACC GAAACGATGA TCTGAAACGG 
ATGTACTCCT TGGTCGCCCT CTCGTCCAAA AACCTGACAG ATCTCAAGTC CATCCTTGAG 
AACCATATAC TCCACCAGGG CACGGAGGCC ATTGCCAAGT GTTGCACTAC CGACGCGGCC 
AACGATCCCA AGACGTACGT ACAGACCATC CTGGACGTGC ACAAGAAGTA CAACGCCCTC 
GTTCTGACGG CGTTCAACAA TGACAACGGA TTTGTGGCCG CTTTGGACAA GGCGTGTGGA 
AAGTTCATAA ACTCGAACGT GGTCACGATC GCAAACAGTG CTAGCAAGTC GCCCGAATTG 
CTGGCCAAGT ACTGCGATCT GTTATTAAAA AAATCCTCGA AAAATCCCGA GGACAAGGAG 
CTCGAGGACA ATCTTAACCA GGTGATGGTC GTGTTCAAGT ATATCGAGGA TAAGGACGTC 
TTCCAAAAAT ATTACTCCAA GATGCTTGCT AAGCGCTTGG TTAACCACAC ATCGGCTTCG 
GATGACGCCG AGGCTATGAT GATCTCCAAA CTGAAGCAGA CATGTGGTTA CGAATACACC 
GTCAAGCTTC AGAGGATGTT CCAGGACATT GGCGTCTCAA AGGACTTGAA CTCGTACTTT 
AAGCAGTACC TGGCCGAAAA GAACTTGACC ATGGAAATAG ACTTTGGCAT CGAAGTCCTG 
TCCTCAGGCT CTTGGCCTTT CCAGCTGAGC AACAACTTCC TGCTACCATC TGAGCTGGAG 
CGGTCAGTGC GACAGTTCAA TGAGTTCTAC GCCGCCCGCC ACTCTGGCCG CAAGCTCAAC 
TGGTTGTATC AGATGTGCAA GGGCGAACTG ATAATGAACG TCAACCGCAA TAATTCCTCC 
ACCTACACCC TGCAGGCCAG CACCTTCCAA ATGTCAGTGC TGCTCCAGTT TAACGATCAG 
CTCAGTTTTA CAGTCCAGCA ACTGCAGGAC AACACACAGA CGCAGCAGGA GAACTTGATT 
CAGGTATTGC AAATACTGCT CAAGGCGAAG GTGCTGACCT CCAGCGACAA TGAGAACTCG 
CTGACGCCAG AGTCGACGGT GGAGCTGTTC CTCGATTACA AAAACAAGAA GCGCCGAATT 
AACATCAACC AGCCTTTGAA GACTGAACTT AAAGTGGAGC AGGAGACTGT ACATAAGCAC 
ATAGAGGAGG ACCGAAAATT ACTGATCCAG GCGGCGATCG TCCGCATCAT GAAGATGCGC 
AAACGACTCA ACCACACCAA TCTCATATCG GAGGTGCTTA ATCAGCTGTC AACGCGCTTT 
AAGCCTAAGG TGCCCGTGAT CAAGAAGTGC ATAGACATCC TGATCGAGAA GGAGTATCTA 
GAGCGAATGG AGGGACACAA AGACACATAT AGTTATCTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul1 ( NM_165570.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724622.1 Drosophila melanogaster cullin 1, transcript variant B (Cul1), mRNA.

Price & Availability↑

CloneID	OFa09213
Clone ID Related Accession (Same CDS sequence)	NM_078931.4 , NM_165569.2 , NM_165571.2 , NM_165570.3
Accession Version	NM_165570.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165570.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCGCT CCGGCAATTC CCAGACCACA CAGAAACTCG TCAACCTGGA CGATATCTGG 
AGCGAATTGG TCGAGGGCAT TATGCAGGTG TTTGAGCACG AGAAGTCCCT CACCCGCAGC 
CAGTACATGC GTTTCTACAC TCACGTCTAT GACTACTGCA CCAGCGTGAG CGCGGCTCCA 
AGCGGACGGA GCAGCGGAAA GACAGGCGGA GCGCAGCTGG TGGGCAAGAA GCTGTACGAC 
CGGCTGGAAC AATTTCTCAA GTCATACCTG AGCGAGCTGC TCACCAAGTT CAAGGCCATC 
AGCGGCGAGG AGGTACTCCT GTCGCGCTAC ACCAAACAGT GGAAGTCGTA TCAGTTCTCC 
AGTACCGTTC TGGACGGCAT CTGCAACTAC CTGAATCGGA ACTGGGTGAA GCGCGAGTGC 
GAGGAGGGGC AGAAGGGTAT CTACAAGATC TACCGACTGG CCCTCGTCGC CTGGAAGGGA 
CATTTGTTCC AAGTGCTAAA TGAGCCGGTC ACCAAGGCAG TGCTCAAGTC CATTGAGGAG 
GAGCGTCAAG GCAAGCTCAT TAACCGGTCG CTGGTCCGCG ACGTTATCGA GTGCTACGTG 
GAGCTCAGCT TTAACGAGGA GGATACCGAT GCCGAGCAGC AGAAGTTATC CGTCTACAAG 
CAAAACTTTG AAAACAAGTT CATCGCAGAC ACATCAGCCT TCTACGAGAA GGAGTCGGAC 
GCCTTTCTGT CCACCAACAC GGTCACCGAG TATCTGAAGC ACGTCGAAAA CAGGCTGGAG 
GAGGAGACGC AGCGCGTCCG CGGCTTTAAT TCAAAGAACG GTCTTTCCTA TCTGCACGAG 
ACCACAGCGG ATGTGCTGAA GTCCACATGC GAAGAGGTTC TTATCGAAAA ACATCTGAAA 
ATCTTTCACA CCGAGTTTCA GAATCTGCTC AACGCAGACC GAAACGATGA TCTGAAACGG 
ATGTACTCCT TGGTCGCCCT CTCGTCCAAA AACCTGACAG ATCTCAAGTC CATCCTTGAG 
AACCATATAC TCCACCAGGG CACGGAGGCC ATTGCCAAGT GTTGCACTAC CGACGCGGCC 
AACGATCCCA AGACGTACGT ACAGACCATC CTGGACGTGC ACAAGAAGTA CAACGCCCTC 
GTTCTGACGG CGTTCAACAA TGACAACGGA TTTGTGGCCG CTTTGGACAA GGCGTGTGGA 
AAGTTCATAA ACTCGAACGT GGTCACGATC GCAAACAGTG CTAGCAAGTC GCCCGAATTG 
CTGGCCAAGT ACTGCGATCT GTTATTAAAA AAATCCTCGA AAAATCCCGA GGACAAGGAG 
CTCGAGGACA ATCTTAACCA GGTGATGGTC GTGTTCAAGT ATATCGAGGA TAAGGACGTC 
TTCCAAAAAT ATTACTCCAA GATGCTTGCT AAGCGCTTGG TTAACCACAC ATCGGCTTCG 
GATGACGCCG AGGCTATGAT GATCTCCAAA CTGAAGCAGA CATGTGGTTA CGAATACACC 
GTCAAGCTTC AGAGGATGTT CCAGGACATT GGCGTCTCAA AGGACTTGAA CTCGTACTTT 
AAGCAGTACC TGGCCGAAAA GAACTTGACC ATGGAAATAG ACTTTGGCAT CGAAGTCCTG 
TCCTCAGGCT CTTGGCCTTT CCAGCTGAGC AACAACTTCC TGCTACCATC TGAGCTGGAG 
CGGTCAGTGC GACAGTTCAA TGAGTTCTAC GCCGCCCGCC ACTCTGGCCG CAAGCTCAAC 
TGGTTGTATC AGATGTGCAA GGGCGAACTG ATAATGAACG TCAACCGCAA TAATTCCTCC 
ACCTACACCC TGCAGGCCAG CACCTTCCAA ATGTCAGTGC TGCTCCAGTT TAACGATCAG 
CTCAGTTTTA CAGTCCAGCA ACTGCAGGAC AACACACAGA CGCAGCAGGA GAACTTGATT 
CAGGTATTGC AAATACTGCT CAAGGCGAAG GTGCTGACCT CCAGCGACAA TGAGAACTCG 
CTGACGCCAG AGTCGACGGT GGAGCTGTTC CTCGATTACA AAAACAAGAA GCGCCGAATT 
AACATCAACC AGCCTTTGAA GACTGAACTT AAAGTGGAGC AGGAGACTGT ACATAAGCAC 
ATAGAGGAGG ACCGAAAATT ACTGATCCAG GCGGCGATCG TCCGCATCAT GAAGATGCGC 
AAACGACTCA ACCACACCAA TCTCATATCG GAGGTGCTTA ATCAGCTGTC AACGCGCTTT 
AAGCCTAAGG TGCCCGTGAT CAAGAAGTGC ATAGACATCC TGATCGAGAA GGAGTATCTA 
GAGCGAATGG AGGGACACAA AGACACATAT AGTTATCTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724622.1
CDS	388..2712
Translation	MNRSGNSQTTQKLVNLDDIWSELVEGIMQVFEHEKSLTRSQYMRFYTHVYDYCTSVSAAPSGRSSGKTGGAQLVGKKLYDRLEQFLKSYLSELLTKFKAISGEEVLLSRYTKQWKSYQFSSTVLDGICNYLNRNWVKRECEEGQKGIYKIYRLALVAWKGHLFQVLNEPVTKAVLKSIEEERQGKLINRSLVRDVIECYVELSFNEEDTDAEQQKLSVYKQNFENKFIADTSAFYEKESDAFLSTNTVTEYLKHVENRLEEETQRVRGFNSKNGLSYLHETTADVLKSTCEEVLIEKHLKIFHTEFQNLLNADRNDDLKRMYSLVALSSKNLTDLKSILENHILHQGTEAIAKCCTTDAANDPKTYVQTILDVHKKYNALVLTAFNNDNGFVAALDKACGKFINSNVVTIANSASKSPELLAKYCDLLLKKSSKNPEDKELEDNLNQVMVVFKYIEDKDVFQKYYSKMLAKRLVNHTSASDDAEAMMISKLKQTCGYEYTVKLQRMFQDIGVSKDLNSYFKQYLAEKNLTMEIDFGIEVLSSGSWPFQLSNNFLLPSELERSVRQFNEFYAARHSGRKLNWLYQMCKGELIMNVNRNNSSTYTLQASTFQMSVLLQFNDQLSFTVQQLQDNTQTQQENLIQVLQILLKAKVLTSSDNENSLTPESTVELFLDYKNKKRRININQPLKTELKVEQETVHKHIEEDRKLLIQAAIVRIMKMRKRLNHTNLISEVLNQLSTRFKPKVPVIKKCIDILIEKEYLERMEGHKDTYSYLA

Target ORF information:

RefSeq Version	NM_165570.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 1, transcript variant B (Cul1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165570.3

ORF Insert Sequence:

ATGAACCGCT CCGGCAATTC CCAGACCACA CAGAAACTCG TCAACCTGGA CGATATCTGG 
AGCGAATTGG TCGAGGGCAT TATGCAGGTG TTTGAGCACG AGAAGTCCCT CACCCGCAGC 
CAGTACATGC GTTTCTACAC TCACGTCTAT GACTACTGCA CCAGCGTGAG CGCGGCTCCA 
AGCGGACGGA GCAGCGGAAA GACAGGCGGA GCGCAGCTGG TGGGCAAGAA GCTGTACGAC 
CGGCTGGAAC AATTTCTCAA GTCATACCTG AGCGAGCTGC TCACCAAGTT CAAGGCCATC 
AGCGGCGAGG AGGTACTCCT GTCGCGCTAC ACCAAACAGT GGAAGTCGTA TCAGTTCTCC 
AGTACCGTTC TGGACGGCAT CTGCAACTAC CTGAATCGGA ACTGGGTGAA GCGCGAGTGC 
GAGGAGGGGC AGAAGGGTAT CTACAAGATC TACCGACTGG CCCTCGTCGC CTGGAAGGGA 
CATTTGTTCC AAGTGCTAAA TGAGCCGGTC ACCAAGGCAG TGCTCAAGTC CATTGAGGAG 
GAGCGTCAAG GCAAGCTCAT TAACCGGTCG CTGGTCCGCG ACGTTATCGA GTGCTACGTG 
GAGCTCAGCT TTAACGAGGA GGATACCGAT GCCGAGCAGC AGAAGTTATC CGTCTACAAG 
CAAAACTTTG AAAACAAGTT CATCGCAGAC ACATCAGCCT TCTACGAGAA GGAGTCGGAC 
GCCTTTCTGT CCACCAACAC GGTCACCGAG TATCTGAAGC ACGTCGAAAA CAGGCTGGAG 
GAGGAGACGC AGCGCGTCCG CGGCTTTAAT TCAAAGAACG GTCTTTCCTA TCTGCACGAG 
ACCACAGCGG ATGTGCTGAA GTCCACATGC GAAGAGGTTC TTATCGAAAA ACATCTGAAA 
ATCTTTCACA CCGAGTTTCA GAATCTGCTC AACGCAGACC GAAACGATGA TCTGAAACGG 
ATGTACTCCT TGGTCGCCCT CTCGTCCAAA AACCTGACAG ATCTCAAGTC CATCCTTGAG 
AACCATATAC TCCACCAGGG CACGGAGGCC ATTGCCAAGT GTTGCACTAC CGACGCGGCC 
AACGATCCCA AGACGTACGT ACAGACCATC CTGGACGTGC ACAAGAAGTA CAACGCCCTC 
GTTCTGACGG CGTTCAACAA TGACAACGGA TTTGTGGCCG CTTTGGACAA GGCGTGTGGA 
AAGTTCATAA ACTCGAACGT GGTCACGATC GCAAACAGTG CTAGCAAGTC GCCCGAATTG 
CTGGCCAAGT ACTGCGATCT GTTATTAAAA AAATCCTCGA AAAATCCCGA GGACAAGGAG 
CTCGAGGACA ATCTTAACCA GGTGATGGTC GTGTTCAAGT ATATCGAGGA TAAGGACGTC 
TTCCAAAAAT ATTACTCCAA GATGCTTGCT AAGCGCTTGG TTAACCACAC ATCGGCTTCG 
GATGACGCCG AGGCTATGAT GATCTCCAAA CTGAAGCAGA CATGTGGTTA CGAATACACC 
GTCAAGCTTC AGAGGATGTT CCAGGACATT GGCGTCTCAA AGGACTTGAA CTCGTACTTT 
AAGCAGTACC TGGCCGAAAA GAACTTGACC ATGGAAATAG ACTTTGGCAT CGAAGTCCTG 
TCCTCAGGCT CTTGGCCTTT CCAGCTGAGC AACAACTTCC TGCTACCATC TGAGCTGGAG 
CGGTCAGTGC GACAGTTCAA TGAGTTCTAC GCCGCCCGCC ACTCTGGCCG CAAGCTCAAC 
TGGTTGTATC AGATGTGCAA GGGCGAACTG ATAATGAACG TCAACCGCAA TAATTCCTCC 
ACCTACACCC TGCAGGCCAG CACCTTCCAA ATGTCAGTGC TGCTCCAGTT TAACGATCAG 
CTCAGTTTTA CAGTCCAGCA ACTGCAGGAC AACACACAGA CGCAGCAGGA GAACTTGATT 
CAGGTATTGC AAATACTGCT CAAGGCGAAG GTGCTGACCT CCAGCGACAA TGAGAACTCG 
CTGACGCCAG AGTCGACGGT GGAGCTGTTC CTCGATTACA AAAACAAGAA GCGCCGAATT 
AACATCAACC AGCCTTTGAA GACTGAACTT AAAGTGGAGC AGGAGACTGT ACATAAGCAC 
ATAGAGGAGG ACCGAAAATT ACTGATCCAG GCGGCGATCG TCCGCATCAT GAAGATGCGC 
AAACGACTCA ACCACACCAA TCTCATATCG GAGGTGCTTA ATCAGCTGTC AACGCGCTTT 
AAGCCTAAGG TGCCCGTGAT CAAGAAGTGC ATAGACATCC TGATCGAGAA GGAGTATCTA 
GAGCGAATGG AGGGACACAA AGACACATAT AGTTATCTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cul1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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