CG9422 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG9422
Entrez Gene ID	35574
Full Name	CG9422 gene product from transcript CG9422-RC
Synonyms	BcDNA:RE35072,Dmel\CG9422
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42C4-42C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_136383.4	NP_610227.2	uncharacterized protein, isoform C
NM_165484.3	NP_724490.1	uncharacterized protein, isoform A
NM_001259221.2	NP_001246150.1	uncharacterized protein, isoform D

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RPP25L	XP_001161736.1
Bos taurus (cattle)	RPP25L	NP_001039576.1
Mus musculus (house mouse)	Rpp25l	NP_081554.2
Danio rerio (zebrafish)	rpp25l	NP_001002540.1
Drosophila melanogaster (fruit fly)	CG9422	NP_724490.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007301	XP_308518.4
	RPP25L	XP_001096868.1
Gallus gallus (chicken)	C9ORF23	XP_424977.1
Homo sapiens (human)	RPP25L	NP_680545.1
Rattus norvegicus (Norway rat)	Rpp25l	NP_001100118.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG9422 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG9422 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09045	NM_165484.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG9422), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09045	NM_136383.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG9422), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09045	NM_001259221.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG9422), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG9422 ( NM_136383.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610227.2 Drosophila melanogaster uncharacterized protein, transcript variant C (CG9422), mRNA.

Price & Availability↑

CloneID	OFa09045
Clone ID Related Accession (Same CDS sequence)	NM_136383.4 , NM_165484.3 , NM_001259221.2
Accession Version	NM_136383.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136383.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCACT ACCGCAAGGC CGAGAATGTG GAGAAGGAGC TGAGCAAGAG CGATCTGCCA 
TTCGAGGACT GCATGCCAAA GTCCCAAAAG GATTTTTTGT GGATGCATGT AAAAGGCGGT 
ACCAAAGTGA GCAATGTGAT TGAGTTTGCT CAGGAGGCGC TGAACAAGGG CGAGCACAGA 
TGCGTGGTGT GGAGCGGATC CGGCGGAGGA GTGGTGAAGA CCATATCCTG TGCCGAGGTC 
CTCAAGCGGA GCCACCCCCT CTACCAGGTG ACGCGCATGG CCTACACCAG TGTGGAGGAG 
CACTGGAAGC CGCAAATGGA AGGCCTGGAG GAGATTATAG TCACACGCCA AATACCCACC 
CTGCACATCC TCATGAGTCT GGACGAGCTG CCGGATACTA TAGATGGCTT ACAAAAGCCA 
AATACGTCCA CTGATTTTTG GGATGGCGGA GGAGCACAGC AACAGCCACA TCCACGTTCC 
CAGCCCCGCC ATCAGCAGCA GCCACATAAA CCAGGGGCTG GGCGTGGGGG TCGGCCGAAC 
AAACGGACCA GACCCGGACG CAATAAACCA GGCCAACAAC CCGAAAAACC TGCCGCTGAA 
GAGAATTTAC CCGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610227.2
CDS	112..732
Translation	MMHYRKAENVEKELSKSDLPFEDCMPKSQKDFLWMHVKGGTKVSNVIEFAQEALNKGEHRCVVWSGSGGGVVKTISCAEVLKRSHPLYQVTRMAYTSVEEHWKPQMEGLEEIIVTRQIPTLHILMSLDELPDTIDGLQKPNTSTDFWDGGGAQQQPHPRSQPRHQQQPHKPGAGRGGRPNKRTRPGRNKPGQQPEKPAAEENLPAS

Target ORF information:

RefSeq Version	NM_136383.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG9422), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136383.4

ORF Insert Sequence:

ATGATGCACT ACCGCAAGGC CGAGAATGTG GAGAAGGAGC TGAGCAAGAG CGATCTGCCA 
TTCGAGGACT GCATGCCAAA GTCCCAAAAG GATTTTTTGT GGATGCATGT AAAAGGCGGT 
ACCAAAGTGA GCAATGTGAT TGAGTTTGCT CAGGAGGCGC TGAACAAGGG CGAGCACAGA 
TGCGTGGTGT GGAGCGGATC CGGCGGAGGA GTGGTGAAGA CCATATCCTG TGCCGAGGTC 
CTCAAGCGGA GCCACCCCCT CTACCAGGTG ACGCGCATGG CCTACACCAG TGTGGAGGAG 
CACTGGAAGC CGCAAATGGA AGGCCTGGAG GAGATTATAG TCACACGCCA AATACCCACC 
CTGCACATCC TCATGAGTCT GGACGAGCTG CCGGATACTA TAGATGGCTT ACAAAAGCCA 
AATACGTCCA CTGATTTTTG GGATGGCGGA GGAGCACAGC AACAGCCACA TCCACGTTCC 
CAGCCCCGCC ATCAGCAGCA GCCACATAAA CCAGGGGCTG GGCGTGGGGG TCGGCCGAAC 
AAACGGACCA GACCCGGACG CAATAAACCA GGCCAACAAC CCGAAAAACC TGCCGCTGAA 
GAGAATTTAC CCGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG9422 ( NM_165484.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724490.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG9422), mRNA.

Price & Availability↑

CloneID	OFa09045
Clone ID Related Accession (Same CDS sequence)	NM_136383.4 , NM_165484.3 , NM_001259221.2
Accession Version	NM_165484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165484.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCACT ACCGCAAGGC CGAGAATGTG GAGAAGGAGC TGAGCAAGAG CGATCTGCCA 
TTCGAGGACT GCATGCCAAA GTCCCAAAAG GATTTTTTGT GGATGCATGT AAAAGGCGGT 
ACCAAAGTGA GCAATGTGAT TGAGTTTGCT CAGGAGGCGC TGAACAAGGG CGAGCACAGA 
TGCGTGGTGT GGAGCGGATC CGGCGGAGGA GTGGTGAAGA CCATATCCTG TGCCGAGGTC 
CTCAAGCGGA GCCACCCCCT CTACCAGGTG ACGCGCATGG CCTACACCAG TGTGGAGGAG 
CACTGGAAGC CGCAAATGGA AGGCCTGGAG GAGATTATAG TCACACGCCA AATACCCACC 
CTGCACATCC TCATGAGTCT GGACGAGCTG CCGGATACTA TAGATGGCTT ACAAAAGCCA 
AATACGTCCA CTGATTTTTG GGATGGCGGA GGAGCACAGC AACAGCCACA TCCACGTTCC 
CAGCCCCGCC ATCAGCAGCA GCCACATAAA CCAGGGGCTG GGCGTGGGGG TCGGCCGAAC 
AAACGGACCA GACCCGGACG CAATAAACCA GGCCAACAAC CCGAAAAACC TGCCGCTGAA 
GAGAATTTAC CCGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724490.1
CDS	112..732
Translation	MMHYRKAENVEKELSKSDLPFEDCMPKSQKDFLWMHVKGGTKVSNVIEFAQEALNKGEHRCVVWSGSGGGVVKTISCAEVLKRSHPLYQVTRMAYTSVEEHWKPQMEGLEEIIVTRQIPTLHILMSLDELPDTIDGLQKPNTSTDFWDGGGAQQQPHPRSQPRHQQQPHKPGAGRGGRPNKRTRPGRNKPGQQPEKPAAEENLPAS

Target ORF information:

RefSeq Version	NM_165484.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG9422), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165484.3

ORF Insert Sequence:

ATGATGCACT ACCGCAAGGC CGAGAATGTG GAGAAGGAGC TGAGCAAGAG CGATCTGCCA 
TTCGAGGACT GCATGCCAAA GTCCCAAAAG GATTTTTTGT GGATGCATGT AAAAGGCGGT 
ACCAAAGTGA GCAATGTGAT TGAGTTTGCT CAGGAGGCGC TGAACAAGGG CGAGCACAGA 
TGCGTGGTGT GGAGCGGATC CGGCGGAGGA GTGGTGAAGA CCATATCCTG TGCCGAGGTC 
CTCAAGCGGA GCCACCCCCT CTACCAGGTG ACGCGCATGG CCTACACCAG TGTGGAGGAG 
CACTGGAAGC CGCAAATGGA AGGCCTGGAG GAGATTATAG TCACACGCCA AATACCCACC 
CTGCACATCC TCATGAGTCT GGACGAGCTG CCGGATACTA TAGATGGCTT ACAAAAGCCA 
AATACGTCCA CTGATTTTTG GGATGGCGGA GGAGCACAGC AACAGCCACA TCCACGTTCC 
CAGCCCCGCC ATCAGCAGCA GCCACATAAA CCAGGGGCTG GGCGTGGGGG TCGGCCGAAC 
AAACGGACCA GACCCGGACG CAATAAACCA GGCCAACAAC CCGAAAAACC TGCCGCTGAA 
GAGAATTTAC CCGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG9422 ( NM_001259221.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246150.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG9422), mRNA.

Price & Availability↑

CloneID	OFa09045
Clone ID Related Accession (Same CDS sequence)	NM_136383.4 , NM_165484.3 , NM_001259221.2
Accession Version	NM_001259221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259221.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCACT ACCGCAAGGC CGAGAATGTG GAGAAGGAGC TGAGCAAGAG CGATCTGCCA 
TTCGAGGACT GCATGCCAAA GTCCCAAAAG GATTTTTTGT GGATGCATGT AAAAGGCGGT 
ACCAAAGTGA GCAATGTGAT TGAGTTTGCT CAGGAGGCGC TGAACAAGGG CGAGCACAGA 
TGCGTGGTGT GGAGCGGATC CGGCGGAGGA GTGGTGAAGA CCATATCCTG TGCCGAGGTC 
CTCAAGCGGA GCCACCCCCT CTACCAGGTG ACGCGCATGG CCTACACCAG TGTGGAGGAG 
CACTGGAAGC CGCAAATGGA AGGCCTGGAG GAGATTATAG TCACACGCCA AATACCCACC 
CTGCACATCC TCATGAGTCT GGACGAGCTG CCGGATACTA TAGATGGCTT ACAAAAGCCA 
AATACGTCCA CTGATTTTTG GGATGGCGGA GGAGCACAGC AACAGCCACA TCCACGTTCC 
CAGCCCCGCC ATCAGCAGCA GCCACATAAA CCAGGGGCTG GGCGTGGGGG TCGGCCGAAC 
AAACGGACCA GACCCGGACG CAATAAACCA GGCCAACAAC CCGAAAAACC TGCCGCTGAA 
GAGAATTTAC CCGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246150.1
CDS	606..1226
Translation	MMHYRKAENVEKELSKSDLPFEDCMPKSQKDFLWMHVKGGTKVSNVIEFAQEALNKGEHRCVVWSGSGGGVVKTISCAEVLKRSHPLYQVTRMAYTSVEEHWKPQMEGLEEIIVTRQIPTLHILMSLDELPDTIDGLQKPNTSTDFWDGGGAQQQPHPRSQPRHQQQPHKPGAGRGGRPNKRTRPGRNKPGQQPEKPAAEENLPAS

Target ORF information:

RefSeq Version	NM_001259221.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG9422), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259221.2

ORF Insert Sequence:

ATGATGCACT ACCGCAAGGC CGAGAATGTG GAGAAGGAGC TGAGCAAGAG CGATCTGCCA 
TTCGAGGACT GCATGCCAAA GTCCCAAAAG GATTTTTTGT GGATGCATGT AAAAGGCGGT 
ACCAAAGTGA GCAATGTGAT TGAGTTTGCT CAGGAGGCGC TGAACAAGGG CGAGCACAGA 
TGCGTGGTGT GGAGCGGATC CGGCGGAGGA GTGGTGAAGA CCATATCCTG TGCCGAGGTC 
CTCAAGCGGA GCCACCCCCT CTACCAGGTG ACGCGCATGG CCTACACCAG TGTGGAGGAG 
CACTGGAAGC CGCAAATGGA AGGCCTGGAG GAGATTATAG TCACACGCCA AATACCCACC 
CTGCACATCC TCATGAGTCT GGACGAGCTG CCGGATACTA TAGATGGCTT ACAAAAGCCA 
AATACGTCCA CTGATTTTTG GGATGGCGGA GGAGCACAGC AACAGCCACA TCCACGTTCC 
CAGCCCCGCC ATCAGCAGCA GCCACATAAA CCAGGGGCTG GGCGTGGGGG TCGGCCGAAC 
AAACGGACCA GACCCGGACG CAATAAACCA GGCCAACAAC CCGAAAAACC TGCCGCTGAA 
GAGAATTTAC CCGCCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG9422 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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