EcR cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	EcR
Entrez Gene ID	35540
Full Name	Ecdysone receptor
Synonyms	CG1765,CG8347,DEcR,Dhr23,DmEcR,Dmel\CG1765,ECR,ECR-C,EcR-A,EcR-B,EcR-B1,EcR1b,EcRA,EcRB,EcRB-1,EcRB1,EcRC,EcdR,Ecr,Ecr1B,NR1H1,anon-WO0229075.1,dECR,dmEcR,ecr,lie,ms(2)06410,ms(2)42A,snt
General protein information	Preferred Names Ecdysone receptor Names CG1765 gene product from transcript CG1765-RG CG1765-PA CG1765-PB CG1765-PC CG1765-PD CG1765-PE CG1765-PG EcR-PA EcR-PB EcR-PC EcR-PD EcR-PE EcR-PG ecdyson receptor 1b ecdysone receptor ecdysone receptor complex ecdysteroid receptor ecdysterone receptor long island expressway male sterile(2)42A snaggletooth
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42A9-42A12

mRNA and Protein(s)

mRNA	Protein	Name
NM_165461.3	NP_724456.1	ecdysone receptor, isoform A
NM_165463.2	NP_724458.1	ecdysone receptor, isoform E
NM_001169590.2	NP_001163061.1	ecdysone receptor, isoform G
NM_165462.2	NP_724457.1	ecdysone receptor, isoform D
NM_165464.3	NP_724459.1	ecdysone receptor, isoform C
NM_165465.3	NP_724460.1	ecdysone receptor, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	NR1H2	NP_009052.3
Xenopus tropicalis (tropical clawed frog)	nr1h2	NP_001072853.1
Rattus norvegicus (Norway rat)	Nr1h2	NP_113814.1
Pan troglodytes (chimpanzee)	NR1H2	XP_512838.4
Canis lupus familiaris (dog)	NR1H2	XP_851316.1
Mus musculus (house mouse)	Nr1h2	NP_001272447.1
Drosophila melanogaster (fruit fly)	EcR	NP_724456.1
	NR1H2	XP_001115995.2
Bos taurus (cattle)	NR1H2	NP_001014883.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012211	XP_320323.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ur data point to a novel ecdysone receptor that does not include Ultraspiracle but is probably comprised of EcR isoforms in Drosophila male accessory glands. Our data suggest that this novel ecdysone receptor might act downstream of homeodomain transcription factor paired (prd) in the male accessory gland
Title: Functional male accessory glands and fertility in Drosophila require novel ecdysone receptor.

Polytene chromosome stains showed that Cg co-localizes with EcR, the major regulator of BR-C, at the BR-C locus and that EcR binding to chromatin was sensitive to changes in Cg levels
Title: Combgap Promotes Ovarian Niche Development and Chromatin Association of EcR-Binding Regions in BR-C.

Context-dependent functional properties of EcR and USP receptors in Drosophila cell survival.
Title: Ligand-independent requirements of steroid receptors EcR and USP for cell survival.

CDK8-CycC may serve as transcriptional cofactors for EcR-dependent transcription.
Title: CDK8-Cyclin C Mediates Nutritional Regulation of Developmental Transitions through the Ecdysone Receptor in Drosophila.

Data show that ecdysone receptors EcRA and EcRB1 are expressed by third instar larvae in the primary nociceptor neurons.
Title: Steroid Receptor Isoform Expression in Drosophila Nociceptor Neurons Is Required for Normal Dendritic Arbor and Sensitivity.

The following EcR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EcR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09008	NM_165465.3 Latest version!	Drosophila melanogaster ecdysone receptor, transcript variant B (EcR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09008	NM_001169590.2 Latest version!	Drosophila melanogaster ecdysone receptor, transcript variant G (EcR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09009	NM_165463.2 Latest version!	Drosophila melanogaster ecdysone receptor, transcript variant E (EcR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09009	NM_165462.2 Latest version!	Drosophila melanogaster ecdysone receptor, transcript variant D (EcR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09009	NM_165461.3 Latest version!	Drosophila melanogaster ecdysone receptor, transcript variant A (EcR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09010	NM_165464.3 Latest version!	Drosophila melanogaster ecdysone receptor, transcript variant C (EcR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EcR ( NM_001169590.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163061.1 Drosophila melanogaster ecdysone receptor, transcript variant G (EcR), mRNA.

Price & Availability↑

CloneID	OFa09008
Clone ID Related Accession (Same CDS sequence)	NM_001169590.2 , NM_165465.3
Accession Version	NM_001169590.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecdysone receptor, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169590.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCGGC GCTGGTCGAA CAACGGCGGC TTCATGCGCC TACCGGAGGA GTCGTCCTCG 
GAGGTCACGT CCTCCTCGAA CGGGCTCGTC CTGCCCTCGG GGGTGAACAT GTCGCCCTCG 
TCGCTGGACT CGCACGACTA TTGCGATCAG GACCTTTGGC TCTGCGGCAA CGAGTCCGGT 
TCGTTTGGCG GCTCCAACGG CCATGGCCTA AGTCAGCAGC AGCAGAGCGT CATCACGCTG 
GCCATGCACG GGTGCTCCAG CACTCTGCCC GCGCAGACAA CCATCATTCC GATCAACGGC 
AACGCGAATG GGAATGGAGG CTCCACCAAT GGCCAATATG TGCCGGGTGC CACTAATCTG 
GGAGCGTTGG CCAACGGGAT GCTCAATGGG GGCTTCAATG GAATGCAGCA ACAGATTCAG 
AATGGCCACG GCCTCATCAA CTCCACAACG CCCTCAACGC CGACCACCCC GCTCCACCTT 
CAGCAGAACC TGGGGGGCGC GGGCGGCGGC GGTATCGGGG GAATGGGTAT TCTTCACCAC 
GCGAATGGCA CCCCAAATGG CCTTATCGGA GTTGTGGGAG GCGGCGGCGG AGTAGGTCTT 
GGAGTAGGCG GAGGCGGAGT GGGAGGCCTG GGAATGCAGC ACACACCCCG AAGCGATTCG 
GTGAATTCTA TATCTTCAGG TCGCGATGAT CTCTCGCCTT CGAGCAGCTT GAACGGATAC 
TCGGCGAACG AAAGCTGCGA TGCGAAGAAG AGCAAGAAGG GACCTGCGCC ACGGGTGCAA 
GAGGAGCTGT GCCTGGTTTG CGGCGACAGG GCCTCCGGCT ACCACTACAA CGCCCTCACC 
TGTGAGGGCT GCAAGGGGTT CTTTCGACGC AGCGTTACGA AGAGCGCCGT CTACTGCTGC 
AAGTTCGGGC GCGCCTGCGA AATGGACATG TACATGAGGC GAAAGTGTCA GGAGTGCCGC 
CTGAAAAAGT GCCTGGCCGT GGGTATGCGG CCGGAATGCG TCGTCCCGGA GAACCAATGT 
GCGATGAAGC GGCGCGAAAA GAAGGCCCAG AAGGAGAAGG ACAAAATGAC CACTTCGCCG 
AGCTCTCAGC ATGGCGGCAA TGGCAGCTTG GCCTCTGGTG GCGGCCAAGA CTTTGTTAAG 
AAGGAGATTC TTGACCTTAT GACATGCGAG CCGCCCCAGC ATGCCACTAT TCCGCTACTA 
CCTGATGAAA TATTGGCCAA GTGTCAAGCG CGCAATATAC CTTCCTTAAC GTACAATCAG 
TTGGCCGTTA TATACAAGTT AATTTGGTAC CAGGATGGCT ATGAGCAGCC ATCTGAAGAG 
GATCTCAGGC GTATAATGAG TCAACCCGAT GAGAACGAGA GCCAAACGGA CGTCAGCTTT 
CGGCATATAA CCGAGATAAC CATACTCACG GTCCAGTTGA TTGTTGAGTT TGCTAAAGGT 
CTACCAGCGT TTACAAAGAT ACCCCAGGAG GACCAGATCA CGTTACTAAA GGCCTGCTCG 
TCGGAGGTGA TGATGCTGCG TATGGCACGA CGCTATGACC ACAGCTCGGA CTCAATATTC 
TTCGCGAATA ATAGATCATA TACGCGGGAT TCTTACAAAA TGGCCGGAAT GGCTGATAAC 
ATTGAAGACC TGCTGCATTT CTGCCGCCAA ATGTTCTCGA TGAAGGTGGA CAACGTCGAA 
TACGCGCTTC TCACTGCCAT TGTGATCTTC TCGGACCGGC CGGGCCTGGA GAAGGCCCAA 
CTAGTCGAAG CGATCCAGAG CTACTACATC GACACGCTAC GCATTTATAT ACTCAACCGC 
CACTGCGGCG ACTCAATGAG CCTCGTCTTC TACGCAAAGC TGCTCTCGAT CCTCACCGAG 
CTGCGTACGC TGGGCAACCA GAACGCCGAG ATGTGTTTCT CACTAAAGCT CAAAAACCGC 
AAACTGCCCA AGTTCCTCGA GGAGATCTGG GACGTTCATG CCATCCCGCC ATCGGTCCAG 
TCGCACCTTC AGATTACCCA GGAGGAGAAC GAGCGTCTCG AGCGGGCTGA GCGTATGCGG 
GCATCGGTTG GGGGCGCCAT TACCGCCGGC ATTGATTGCG ACTCTGCCTC CACTTCGGCG 
GCGGCAGCCG CGGCCCAGCA TCAGCCTCAG CCTCAGCCCC AGCCCCAACC CTCCTCCCTG 
ACCCAGAACG ATTCCCAGCA CCAGACACAG CCGCAGCTAC AACCTCAGCT ACCACCTCAG 
CTGCAAGGTC AACTGCAACC CCAGCTCCAA CCACAGCTTC AGACGCAACT CCAGCCACAG 
ATTCAACCAC AGCCACAGCT CCTTCCCGTC TCCGCTCCCG TGCCCGCCTC CGTAACCGCA 
CCTGGTTCCT TGTCCGCGGT CAGTACGAGC AGCGAATACA TGGGCGGAAG TGCGGCCATA 
GGACCCATCA CGCCGGCAAC CACCAGCAGT ATCACGGCTG CCGTTACCGC TAGCTCCACC 
ACATCAGCGG TACCGATGGG CAACGGAGTT GGAGTCGGTG TTGGGGTGGG CGGCAACGTC 
AGCATGTATG CGAACGCCCA GACGGCGATG GCCTTGATGG GTGTAGCCCT GCATTCGCAC 
CAAGAGCAGC TTATCGGGGG AGTGGCGGTT AAGTCGGAGC ACTCGACGAC TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163061.1
CDS	1064..3700
Translation	MKRRWSNNGGFMRLPEESSSEVTSSSNGLVLPSGVNMSPSSLDSHDYCDQDLWLCGNESGSFGGSNGHGLSQQQQSVITLAMHGCSSTLPAQTTIIPINGNANGNGGSTNGQYVPGATNLGALANGMLNGGFNGMQQQIQNGHGLINSTTPSTPTTPLHLQQNLGGAGGGGIGGMGILHHANGTPNGLIGVVGGGGGVGLGVGGGGVGGLGMQHTPRSDSVNSISSGRDDLSPSSSLNGYSANESCDAKKSKKGPAPRVQEELCLVCGDRASGYHYNALTCEGCKGFFRRSVTKSAVYCCKFGRACEMDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGGNGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIYKLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAFTKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIEDLLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHCGDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQSHLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSLTQNDSQHQTQPQLQPQLPPQLQGQLQPQLQPQLQTQLQPQIQPQPQLLPVSAPVPASVTAPGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGGNVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA

Target ORF information:

RefSeq Version	NM_001169590.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecdysone receptor, transcript variant G (EcR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169590.2

ORF Insert Sequence:

ATGAAGCGGC GCTGGTCGAA CAACGGCGGC TTCATGCGCC TACCGGAGGA GTCGTCCTCG 
GAGGTCACGT CCTCCTCGAA CGGGCTCGTC CTGCCCTCGG GGGTGAACAT GTCGCCCTCG 
TCGCTGGACT CGCACGACTA TTGCGATCAG GACCTTTGGC TCTGCGGCAA CGAGTCCGGT 
TCGTTTGGCG GCTCCAACGG CCATGGCCTA AGTCAGCAGC AGCAGAGCGT CATCACGCTG 
GCCATGCACG GGTGCTCCAG CACTCTGCCC GCGCAGACAA CCATCATTCC GATCAACGGC 
AACGCGAATG GGAATGGAGG CTCCACCAAT GGCCAATATG TGCCGGGTGC CACTAATCTG 
GGAGCGTTGG CCAACGGGAT GCTCAATGGG GGCTTCAATG GAATGCAGCA ACAGATTCAG 
AATGGCCACG GCCTCATCAA CTCCACAACG CCCTCAACGC CGACCACCCC GCTCCACCTT 
CAGCAGAACC TGGGGGGCGC GGGCGGCGGC GGTATCGGGG GAATGGGTAT TCTTCACCAC 
GCGAATGGCA CCCCAAATGG CCTTATCGGA GTTGTGGGAG GCGGCGGCGG AGTAGGTCTT 
GGAGTAGGCG GAGGCGGAGT GGGAGGCCTG GGAATGCAGC ACACACCCCG AAGCGATTCG 
GTGAATTCTA TATCTTCAGG TCGCGATGAT CTCTCGCCTT CGAGCAGCTT GAACGGATAC 
TCGGCGAACG AAAGCTGCGA TGCGAAGAAG AGCAAGAAGG GACCTGCGCC ACGGGTGCAA 
GAGGAGCTGT GCCTGGTTTG CGGCGACAGG GCCTCCGGCT ACCACTACAA CGCCCTCACC 
TGTGAGGGCT GCAAGGGGTT CTTTCGACGC AGCGTTACGA AGAGCGCCGT CTACTGCTGC 
AAGTTCGGGC GCGCCTGCGA AATGGACATG TACATGAGGC GAAAGTGTCA GGAGTGCCGC 
CTGAAAAAGT GCCTGGCCGT GGGTATGCGG CCGGAATGCG TCGTCCCGGA GAACCAATGT 
GCGATGAAGC GGCGCGAAAA GAAGGCCCAG AAGGAGAAGG ACAAAATGAC CACTTCGCCG 
AGCTCTCAGC ATGGCGGCAA TGGCAGCTTG GCCTCTGGTG GCGGCCAAGA CTTTGTTAAG 
AAGGAGATTC TTGACCTTAT GACATGCGAG CCGCCCCAGC ATGCCACTAT TCCGCTACTA 
CCTGATGAAA TATTGGCCAA GTGTCAAGCG CGCAATATAC CTTCCTTAAC GTACAATCAG 
TTGGCCGTTA TATACAAGTT AATTTGGTAC CAGGATGGCT ATGAGCAGCC ATCTGAAGAG 
GATCTCAGGC GTATAATGAG TCAACCCGAT GAGAACGAGA GCCAAACGGA CGTCAGCTTT 
CGGCATATAA CCGAGATAAC CATACTCACG GTCCAGTTGA TTGTTGAGTT TGCTAAAGGT 
CTACCAGCGT TTACAAAGAT ACCCCAGGAG GACCAGATCA CGTTACTAAA GGCCTGCTCG 
TCGGAGGTGA TGATGCTGCG TATGGCACGA CGCTATGACC ACAGCTCGGA CTCAATATTC 
TTCGCGAATA ATAGATCATA TACGCGGGAT TCTTACAAAA TGGCCGGAAT GGCTGATAAC 
ATTGAAGACC TGCTGCATTT CTGCCGCCAA ATGTTCTCGA TGAAGGTGGA CAACGTCGAA 
TACGCGCTTC TCACTGCCAT TGTGATCTTC TCGGACCGGC CGGGCCTGGA GAAGGCCCAA 
CTAGTCGAAG CGATCCAGAG CTACTACATC GACACGCTAC GCATTTATAT ACTCAACCGC 
CACTGCGGCG ACTCAATGAG CCTCGTCTTC TACGCAAAGC TGCTCTCGAT CCTCACCGAG 
CTGCGTACGC TGGGCAACCA GAACGCCGAG ATGTGTTTCT CACTAAAGCT CAAAAACCGC 
AAACTGCCCA AGTTCCTCGA GGAGATCTGG GACGTTCATG CCATCCCGCC ATCGGTCCAG 
TCGCACCTTC AGATTACCCA GGAGGAGAAC GAGCGTCTCG AGCGGGCTGA GCGTATGCGG 
GCATCGGTTG GGGGCGCCAT TACCGCCGGC ATTGATTGCG ACTCTGCCTC CACTTCGGCG 
GCGGCAGCCG CGGCCCAGCA TCAGCCTCAG CCTCAGCCCC AGCCCCAACC CTCCTCCCTG 
ACCCAGAACG ATTCCCAGCA CCAGACACAG CCGCAGCTAC AACCTCAGCT ACCACCTCAG 
CTGCAAGGTC AACTGCAACC CCAGCTCCAA CCACAGCTTC AGACGCAACT CCAGCCACAG 
ATTCAACCAC AGCCACAGCT CCTTCCCGTC TCCGCTCCCG TGCCCGCCTC CGTAACCGCA 
CCTGGTTCCT TGTCCGCGGT CAGTACGAGC AGCGAATACA TGGGCGGAAG TGCGGCCATA 
GGACCCATCA CGCCGGCAAC CACCAGCAGT ATCACGGCTG CCGTTACCGC TAGCTCCACC 
ACATCAGCGG TACCGATGGG CAACGGAGTT GGAGTCGGTG TTGGGGTGGG CGGCAACGTC 
AGCATGTATG CGAACGCCCA GACGGCGATG GCCTTGATGG GTGTAGCCCT GCATTCGCAC 
CAAGAGCAGC TTATCGGGGG AGTGGCGGTT AAGTCGGAGC ACTCGACGAC TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EcR ( NM_165465.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724460.1 Drosophila melanogaster ecdysone receptor, transcript variant B (EcR), mRNA.

Price & Availability↑

CloneID	OFa09008
Clone ID Related Accession (Same CDS sequence)	NM_001169590.2 , NM_165465.3
Accession Version	NM_165465.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecdysone receptor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165465.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCGGC GCTGGTCGAA CAACGGCGGC TTCATGCGCC TACCGGAGGA GTCGTCCTCG 
GAGGTCACGT CCTCCTCGAA CGGGCTCGTC CTGCCCTCGG GGGTGAACAT GTCGCCCTCG 
TCGCTGGACT CGCACGACTA TTGCGATCAG GACCTTTGGC TCTGCGGCAA CGAGTCCGGT 
TCGTTTGGCG GCTCCAACGG CCATGGCCTA AGTCAGCAGC AGCAGAGCGT CATCACGCTG 
GCCATGCACG GGTGCTCCAG CACTCTGCCC GCGCAGACAA CCATCATTCC GATCAACGGC 
AACGCGAATG GGAATGGAGG CTCCACCAAT GGCCAATATG TGCCGGGTGC CACTAATCTG 
GGAGCGTTGG CCAACGGGAT GCTCAATGGG GGCTTCAATG GAATGCAGCA ACAGATTCAG 
AATGGCCACG GCCTCATCAA CTCCACAACG CCCTCAACGC CGACCACCCC GCTCCACCTT 
CAGCAGAACC TGGGGGGCGC GGGCGGCGGC GGTATCGGGG GAATGGGTAT TCTTCACCAC 
GCGAATGGCA CCCCAAATGG CCTTATCGGA GTTGTGGGAG GCGGCGGCGG AGTAGGTCTT 
GGAGTAGGCG GAGGCGGAGT GGGAGGCCTG GGAATGCAGC ACACACCCCG AAGCGATTCG 
GTGAATTCTA TATCTTCAGG TCGCGATGAT CTCTCGCCTT CGAGCAGCTT GAACGGATAC 
TCGGCGAACG AAAGCTGCGA TGCGAAGAAG AGCAAGAAGG GACCTGCGCC ACGGGTGCAA 
GAGGAGCTGT GCCTGGTTTG CGGCGACAGG GCCTCCGGCT ACCACTACAA CGCCCTCACC 
TGTGAGGGCT GCAAGGGGTT CTTTCGACGC AGCGTTACGA AGAGCGCCGT CTACTGCTGC 
AAGTTCGGGC GCGCCTGCGA AATGGACATG TACATGAGGC GAAAGTGTCA GGAGTGCCGC 
CTGAAAAAGT GCCTGGCCGT GGGTATGCGG CCGGAATGCG TCGTCCCGGA GAACCAATGT 
GCGATGAAGC GGCGCGAAAA GAAGGCCCAG AAGGAGAAGG ACAAAATGAC CACTTCGCCG 
AGCTCTCAGC ATGGCGGCAA TGGCAGCTTG GCCTCTGGTG GCGGCCAAGA CTTTGTTAAG 
AAGGAGATTC TTGACCTTAT GACATGCGAG CCGCCCCAGC ATGCCACTAT TCCGCTACTA 
CCTGATGAAA TATTGGCCAA GTGTCAAGCG CGCAATATAC CTTCCTTAAC GTACAATCAG 
TTGGCCGTTA TATACAAGTT AATTTGGTAC CAGGATGGCT ATGAGCAGCC ATCTGAAGAG 
GATCTCAGGC GTATAATGAG TCAACCCGAT GAGAACGAGA GCCAAACGGA CGTCAGCTTT 
CGGCATATAA CCGAGATAAC CATACTCACG GTCCAGTTGA TTGTTGAGTT TGCTAAAGGT 
CTACCAGCGT TTACAAAGAT ACCCCAGGAG GACCAGATCA CGTTACTAAA GGCCTGCTCG 
TCGGAGGTGA TGATGCTGCG TATGGCACGA CGCTATGACC ACAGCTCGGA CTCAATATTC 
TTCGCGAATA ATAGATCATA TACGCGGGAT TCTTACAAAA TGGCCGGAAT GGCTGATAAC 
ATTGAAGACC TGCTGCATTT CTGCCGCCAA ATGTTCTCGA TGAAGGTGGA CAACGTCGAA 
TACGCGCTTC TCACTGCCAT TGTGATCTTC TCGGACCGGC CGGGCCTGGA GAAGGCCCAA 
CTAGTCGAAG CGATCCAGAG CTACTACATC GACACGCTAC GCATTTATAT ACTCAACCGC 
CACTGCGGCG ACTCAATGAG CCTCGTCTTC TACGCAAAGC TGCTCTCGAT CCTCACCGAG 
CTGCGTACGC TGGGCAACCA GAACGCCGAG ATGTGTTTCT CACTAAAGCT CAAAAACCGC 
AAACTGCCCA AGTTCCTCGA GGAGATCTGG GACGTTCATG CCATCCCGCC ATCGGTCCAG 
TCGCACCTTC AGATTACCCA GGAGGAGAAC GAGCGTCTCG AGCGGGCTGA GCGTATGCGG 
GCATCGGTTG GGGGCGCCAT TACCGCCGGC ATTGATTGCG ACTCTGCCTC CACTTCGGCG 
GCGGCAGCCG CGGCCCAGCA TCAGCCTCAG CCTCAGCCCC AGCCCCAACC CTCCTCCCTG 
ACCCAGAACG ATTCCCAGCA CCAGACACAG CCGCAGCTAC AACCTCAGCT ACCACCTCAG 
CTGCAAGGTC AACTGCAACC CCAGCTCCAA CCACAGCTTC AGACGCAACT CCAGCCACAG 
ATTCAACCAC AGCCACAGCT CCTTCCCGTC TCCGCTCCCG TGCCCGCCTC CGTAACCGCA 
CCTGGTTCCT TGTCCGCGGT CAGTACGAGC AGCGAATACA TGGGCGGAAG TGCGGCCATA 
GGACCCATCA CGCCGGCAAC CACCAGCAGT ATCACGGCTG CCGTTACCGC TAGCTCCACC 
ACATCAGCGG TACCGATGGG CAACGGAGTT GGAGTCGGTG TTGGGGTGGG CGGCAACGTC 
AGCATGTATG CGAACGCCCA GACGGCGATG GCCTTGATGG GTGTAGCCCT GCATTCGCAC 
CAAGAGCAGC TTATCGGGGG AGTGGCGGTT AAGTCGGAGC ACTCGACGAC TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724460.1
CDS	1145..3781
Translation	MKRRWSNNGGFMRLPEESSSEVTSSSNGLVLPSGVNMSPSSLDSHDYCDQDLWLCGNESGSFGGSNGHGLSQQQQSVITLAMHGCSSTLPAQTTIIPINGNANGNGGSTNGQYVPGATNLGALANGMLNGGFNGMQQQIQNGHGLINSTTPSTPTTPLHLQQNLGGAGGGGIGGMGILHHANGTPNGLIGVVGGGGGVGLGVGGGGVGGLGMQHTPRSDSVNSISSGRDDLSPSSSLNGYSANESCDAKKSKKGPAPRVQEELCLVCGDRASGYHYNALTCEGCKGFFRRSVTKSAVYCCKFGRACEMDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGGNGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIYKLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAFTKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIEDLLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHCGDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQSHLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSLTQNDSQHQTQPQLQPQLPPQLQGQLQPQLQPQLQTQLQPQIQPQPQLLPVSAPVPASVTAPGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGGNVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA

Target ORF information:

RefSeq Version	NM_165465.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecdysone receptor, transcript variant B (EcR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165465.3

ORF Insert Sequence:

ATGAAGCGGC GCTGGTCGAA CAACGGCGGC TTCATGCGCC TACCGGAGGA GTCGTCCTCG 
GAGGTCACGT CCTCCTCGAA CGGGCTCGTC CTGCCCTCGG GGGTGAACAT GTCGCCCTCG 
TCGCTGGACT CGCACGACTA TTGCGATCAG GACCTTTGGC TCTGCGGCAA CGAGTCCGGT 
TCGTTTGGCG GCTCCAACGG CCATGGCCTA AGTCAGCAGC AGCAGAGCGT CATCACGCTG 
GCCATGCACG GGTGCTCCAG CACTCTGCCC GCGCAGACAA CCATCATTCC GATCAACGGC 
AACGCGAATG GGAATGGAGG CTCCACCAAT GGCCAATATG TGCCGGGTGC CACTAATCTG 
GGAGCGTTGG CCAACGGGAT GCTCAATGGG GGCTTCAATG GAATGCAGCA ACAGATTCAG 
AATGGCCACG GCCTCATCAA CTCCACAACG CCCTCAACGC CGACCACCCC GCTCCACCTT 
CAGCAGAACC TGGGGGGCGC GGGCGGCGGC GGTATCGGGG GAATGGGTAT TCTTCACCAC 
GCGAATGGCA CCCCAAATGG CCTTATCGGA GTTGTGGGAG GCGGCGGCGG AGTAGGTCTT 
GGAGTAGGCG GAGGCGGAGT GGGAGGCCTG GGAATGCAGC ACACACCCCG AAGCGATTCG 
GTGAATTCTA TATCTTCAGG TCGCGATGAT CTCTCGCCTT CGAGCAGCTT GAACGGATAC 
TCGGCGAACG AAAGCTGCGA TGCGAAGAAG AGCAAGAAGG GACCTGCGCC ACGGGTGCAA 
GAGGAGCTGT GCCTGGTTTG CGGCGACAGG GCCTCCGGCT ACCACTACAA CGCCCTCACC 
TGTGAGGGCT GCAAGGGGTT CTTTCGACGC AGCGTTACGA AGAGCGCCGT CTACTGCTGC 
AAGTTCGGGC GCGCCTGCGA AATGGACATG TACATGAGGC GAAAGTGTCA GGAGTGCCGC 
CTGAAAAAGT GCCTGGCCGT GGGTATGCGG CCGGAATGCG TCGTCCCGGA GAACCAATGT 
GCGATGAAGC GGCGCGAAAA GAAGGCCCAG AAGGAGAAGG ACAAAATGAC CACTTCGCCG 
AGCTCTCAGC ATGGCGGCAA TGGCAGCTTG GCCTCTGGTG GCGGCCAAGA CTTTGTTAAG 
AAGGAGATTC TTGACCTTAT GACATGCGAG CCGCCCCAGC ATGCCACTAT TCCGCTACTA 
CCTGATGAAA TATTGGCCAA GTGTCAAGCG CGCAATATAC CTTCCTTAAC GTACAATCAG 
TTGGCCGTTA TATACAAGTT AATTTGGTAC CAGGATGGCT ATGAGCAGCC ATCTGAAGAG 
GATCTCAGGC GTATAATGAG TCAACCCGAT GAGAACGAGA GCCAAACGGA CGTCAGCTTT 
CGGCATATAA CCGAGATAAC CATACTCACG GTCCAGTTGA TTGTTGAGTT TGCTAAAGGT 
CTACCAGCGT TTACAAAGAT ACCCCAGGAG GACCAGATCA CGTTACTAAA GGCCTGCTCG 
TCGGAGGTGA TGATGCTGCG TATGGCACGA CGCTATGACC ACAGCTCGGA CTCAATATTC 
TTCGCGAATA ATAGATCATA TACGCGGGAT TCTTACAAAA TGGCCGGAAT GGCTGATAAC 
ATTGAAGACC TGCTGCATTT CTGCCGCCAA ATGTTCTCGA TGAAGGTGGA CAACGTCGAA 
TACGCGCTTC TCACTGCCAT TGTGATCTTC TCGGACCGGC CGGGCCTGGA GAAGGCCCAA 
CTAGTCGAAG CGATCCAGAG CTACTACATC GACACGCTAC GCATTTATAT ACTCAACCGC 
CACTGCGGCG ACTCAATGAG CCTCGTCTTC TACGCAAAGC TGCTCTCGAT CCTCACCGAG 
CTGCGTACGC TGGGCAACCA GAACGCCGAG ATGTGTTTCT CACTAAAGCT CAAAAACCGC 
AAACTGCCCA AGTTCCTCGA GGAGATCTGG GACGTTCATG CCATCCCGCC ATCGGTCCAG 
TCGCACCTTC AGATTACCCA GGAGGAGAAC GAGCGTCTCG AGCGGGCTGA GCGTATGCGG 
GCATCGGTTG GGGGCGCCAT TACCGCCGGC ATTGATTGCG ACTCTGCCTC CACTTCGGCG 
GCGGCAGCCG CGGCCCAGCA TCAGCCTCAG CCTCAGCCCC AGCCCCAACC CTCCTCCCTG 
ACCCAGAACG ATTCCCAGCA CCAGACACAG CCGCAGCTAC AACCTCAGCT ACCACCTCAG 
CTGCAAGGTC AACTGCAACC CCAGCTCCAA CCACAGCTTC AGACGCAACT CCAGCCACAG 
ATTCAACCAC AGCCACAGCT CCTTCCCGTC TCCGCTCCCG TGCCCGCCTC CGTAACCGCA 
CCTGGTTCCT TGTCCGCGGT CAGTACGAGC AGCGAATACA TGGGCGGAAG TGCGGCCATA 
GGACCCATCA CGCCGGCAAC CACCAGCAGT ATCACGGCTG CCGTTACCGC TAGCTCCACC 
ACATCAGCGG TACCGATGGG CAACGGAGTT GGAGTCGGTG TTGGGGTGGG CGGCAACGTC 
AGCATGTATG CGAACGCCCA GACGGCGATG GCCTTGATGG GTGTAGCCCT GCATTCGCAC 
CAAGAGCAGC TTATCGGGGG AGTGGCGGTT AAGTCGGAGC ACTCGACGAC TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EcR ( NM_165461.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724456.1 Drosophila melanogaster ecdysone receptor, transcript variant A (EcR), mRNA.

Price & Availability↑

CloneID	OFa09009
Clone ID Related Accession (Same CDS sequence)	NM_165461.3 , NM_165463.2 , NM_165462.2
Accession Version	NM_165461.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecdysone receptor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165461.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACGA CGAGTGGACA ACAACAGTCA AAGCAAAAGC TGTCGACCTT ACCTAGCCAT 
ATTTTGCTAC AACAACAATT AGCTGCCTCT GCAGGCCCAT CTTCCTCAGT ATCCCTATCC 
CCATCTTCTT CTGCCGCCCT GACCCTGCAT GTAGCCAGTG CTAATGGCGG TGCAAGAGAA 
ACAACTTCTG CAGCCGCAGT CAAGGATAAA CTAAGGCCGA CACCCACAGC CATTAAAATT 
GAGCCCATGC CGGACGTCAT TTCCGTAGGC ACGGTGGCCG GAGGATCTAG CGTAGCTACT 
GTTGTTGCTC CCGCAGCAAC CACAACTTCC AATAAGCCAA ATTCTACAGC GGCGCCCTCG 
ACGTCGGCTG CAGCTGCAAA TGGTCATTTG GTGCTTGTGC CGAACAAAAG ACCGCGACTT 
GATGTTACCG AGGACTGGAT GTCCACTCCT AGTCCAGGCA GCGTGCCCAG CTCTGCGCCG 
CCACTTTCGC CTTCCCCCGG CTCGCAGAAT CACAGCTACA ACATGTCCAA CGGCTATGCA 
TCCCCCATGT CCGCCGGAAG CTATGATCCT TACAGTCCTA CCGGCAAAAC AGGTCGCGAT 
GATCTCTCGC CTTCGAGCAG CTTGAACGGA TACTCGGCGA ACGAAAGCTG CGATGCGAAG 
AAGAGCAAGA AGGGACCTGC GCCACGGGTG CAAGAGGAGC TGTGCCTGGT TTGCGGCGAC 
AGGGCCTCCG GCTACCACTA CAACGCCCTC ACCTGTGAGG GCTGCAAGGG GTTCTTTCGA 
CGCAGCGTTA CGAAGAGCGC CGTCTACTGC TGCAAGTTCG GGCGCGCCTG CGAAATGGAC 
ATGTACATGA GGCGAAAGTG TCAGGAGTGC CGCCTGAAAA AGTGCCTGGC CGTGGGTATG 
CGGCCGGAAT GCGTCGTCCC GGAGAACCAA TGTGCGATGA AGCGGCGCGA AAAGAAGGCC 
CAGAAGGAGA AGGACAAAAT GACCACTTCG CCGAGCTCTC AGCATGGCGG CAATGGCAGC 
TTGGCCTCTG GTGGCGGCCA AGACTTTGTT AAGAAGGAGA TTCTTGACCT TATGACATGC 
GAGCCGCCCC AGCATGCCAC TATTCCGCTA CTACCTGATG AAATATTGGC CAAGTGTCAA 
GCGCGCAATA TACCTTCCTT AACGTACAAT CAGTTGGCCG TTATATACAA GTTAATTTGG 
TACCAGGATG GCTATGAGCA GCCATCTGAA GAGGATCTCA GGCGTATAAT GAGTCAACCC 
GATGAGAACG AGAGCCAAAC GGACGTCAGC TTTCGGCATA TAACCGAGAT AACCATACTC 
ACGGTCCAGT TGATTGTTGA GTTTGCTAAA GGTCTACCAG CGTTTACAAA GATACCCCAG 
GAGGACCAGA TCACGTTACT AAAGGCCTGC TCGTCGGAGG TGATGATGCT GCGTATGGCA 
CGACGCTATG ACCACAGCTC GGACTCAATA TTCTTCGCGA ATAATAGATC ATATACGCGG 
GATTCTTACA AAATGGCCGG AATGGCTGAT AACATTGAAG ACCTGCTGCA TTTCTGCCGC 
CAAATGTTCT CGATGAAGGT GGACAACGTC GAATACGCGC TTCTCACTGC CATTGTGATC 
TTCTCGGACC GGCCGGGCCT GGAGAAGGCC CAACTAGTCG AAGCGATCCA GAGCTACTAC 
ATCGACACGC TACGCATTTA TATACTCAAC CGCCACTGCG GCGACTCAAT GAGCCTCGTC 
TTCTACGCAA AGCTGCTCTC GATCCTCACC GAGCTGCGTA CGCTGGGCAA CCAGAACGCC 
GAGATGTGTT TCTCACTAAA GCTCAAAAAC CGCAAACTGC CCAAGTTCCT CGAGGAGATC 
TGGGACGTTC ATGCCATCCC GCCATCGGTC CAGTCGCACC TTCAGATTAC CCAGGAGGAG 
AACGAGCGTC TCGAGCGGGC TGAGCGTATG CGGGCATCGG TTGGGGGCGC CATTACCGCC 
GGCATTGATT GCGACTCTGC CTCCACTTCG GCGGCGGCAG CCGCGGCCCA GCATCAGCCT 
CAGCCTCAGC CCCAGCCCCA ACCCTCCTCC CTGACCCAGA ACGATTCCCA GCACCAGACA 
CAGCCGCAGC TACAACCTCA GCTACCACCT CAGCTGCAAG GTCAACTGCA ACCCCAGCTC 
CAACCACAGC TTCAGACGCA ACTCCAGCCA CAGATTCAAC CACAGCCACA GCTCCTTCCC 
GTCTCCGCTC CCGTGCCCGC CTCCGTAACC GCACCTGGTT CCTTGTCCGC GGTCAGTACG 
AGCAGCGAAT ACATGGGCGG AAGTGCGGCC ATAGGACCCA TCACGCCGGC AACCACCAGC 
AGTATCACGG CTGCCGTTAC CGCTAGCTCC ACCACATCAG CGGTACCGAT GGGCAACGGA 
GTTGGAGTCG GTGTTGGGGT GGGCGGCAAC GTCAGCATGT ATGCGAACGC CCAGACGGCG 
ATGGCCTTGA TGGGTGTAGC CCTGCATTCG CACCAAGAGC AGCTTATCGG GGGAGTGGCG 
GTTAAGTCGG AGCACTCGAC GACTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724456.1
CDS	390..2939
Translation	MLTTSGQQQSKQKLSTLPSHILLQQQLAASAGPSSSVSLSPSSSAALTLHVASANGGARETTSAAAVKDKLRPTPTAIKIEPMPDVISVGTVAGGSSVATVVAPAATTTSNKPNSTAAPSTSAAAANGHLVLVPNKRPRLDVTEDWMSTPSPGSVPSSAPPLSPSPGSQNHSYNMSNGYASPMSAGSYDPYSPTGKTGRDDLSPSSSLNGYSANESCDAKKSKKGPAPRVQEELCLVCGDRASGYHYNALTCEGCKGFFRRSVTKSAVYCCKFGRACEMDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGGNGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIYKLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAFTKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIEDLLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHCGDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQSHLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSLTQNDSQHQTQPQLQPQLPPQLQGQLQPQLQPQLQTQLQPQIQPQPQLLPVSAPVPASVTAPGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGGNVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA

Target ORF information:

RefSeq Version	NM_165461.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecdysone receptor, transcript variant A (EcR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165461.3

ORF Insert Sequence:

ATGTTGACGA CGAGTGGACA ACAACAGTCA AAGCAAAAGC TGTCGACCTT ACCTAGCCAT 
ATTTTGCTAC AACAACAATT AGCTGCCTCT GCAGGCCCAT CTTCCTCAGT ATCCCTATCC 
CCATCTTCTT CTGCCGCCCT GACCCTGCAT GTAGCCAGTG CTAATGGCGG TGCAAGAGAA 
ACAACTTCTG CAGCCGCAGT CAAGGATAAA CTAAGGCCGA CACCCACAGC CATTAAAATT 
GAGCCCATGC CGGACGTCAT TTCCGTAGGC ACGGTGGCCG GAGGATCTAG CGTAGCTACT 
GTTGTTGCTC CCGCAGCAAC CACAACTTCC AATAAGCCAA ATTCTACAGC GGCGCCCTCG 
ACGTCGGCTG CAGCTGCAAA TGGTCATTTG GTGCTTGTGC CGAACAAAAG ACCGCGACTT 
GATGTTACCG AGGACTGGAT GTCCACTCCT AGTCCAGGCA GCGTGCCCAG CTCTGCGCCG 
CCACTTTCGC CTTCCCCCGG CTCGCAGAAT CACAGCTACA ACATGTCCAA CGGCTATGCA 
TCCCCCATGT CCGCCGGAAG CTATGATCCT TACAGTCCTA CCGGCAAAAC AGGTCGCGAT 
GATCTCTCGC CTTCGAGCAG CTTGAACGGA TACTCGGCGA ACGAAAGCTG CGATGCGAAG 
AAGAGCAAGA AGGGACCTGC GCCACGGGTG CAAGAGGAGC TGTGCCTGGT TTGCGGCGAC 
AGGGCCTCCG GCTACCACTA CAACGCCCTC ACCTGTGAGG GCTGCAAGGG GTTCTTTCGA 
CGCAGCGTTA CGAAGAGCGC CGTCTACTGC TGCAAGTTCG GGCGCGCCTG CGAAATGGAC 
ATGTACATGA GGCGAAAGTG TCAGGAGTGC CGCCTGAAAA AGTGCCTGGC CGTGGGTATG 
CGGCCGGAAT GCGTCGTCCC GGAGAACCAA TGTGCGATGA AGCGGCGCGA AAAGAAGGCC 
CAGAAGGAGA AGGACAAAAT GACCACTTCG CCGAGCTCTC AGCATGGCGG CAATGGCAGC 
TTGGCCTCTG GTGGCGGCCA AGACTTTGTT AAGAAGGAGA TTCTTGACCT TATGACATGC 
GAGCCGCCCC AGCATGCCAC TATTCCGCTA CTACCTGATG AAATATTGGC CAAGTGTCAA 
GCGCGCAATA TACCTTCCTT AACGTACAAT CAGTTGGCCG TTATATACAA GTTAATTTGG 
TACCAGGATG GCTATGAGCA GCCATCTGAA GAGGATCTCA GGCGTATAAT GAGTCAACCC 
GATGAGAACG AGAGCCAAAC GGACGTCAGC TTTCGGCATA TAACCGAGAT AACCATACTC 
ACGGTCCAGT TGATTGTTGA GTTTGCTAAA GGTCTACCAG CGTTTACAAA GATACCCCAG 
GAGGACCAGA TCACGTTACT AAAGGCCTGC TCGTCGGAGG TGATGATGCT GCGTATGGCA 
CGACGCTATG ACCACAGCTC GGACTCAATA TTCTTCGCGA ATAATAGATC ATATACGCGG 
GATTCTTACA AAATGGCCGG AATGGCTGAT AACATTGAAG ACCTGCTGCA TTTCTGCCGC 
CAAATGTTCT CGATGAAGGT GGACAACGTC GAATACGCGC TTCTCACTGC CATTGTGATC 
TTCTCGGACC GGCCGGGCCT GGAGAAGGCC CAACTAGTCG AAGCGATCCA GAGCTACTAC 
ATCGACACGC TACGCATTTA TATACTCAAC CGCCACTGCG GCGACTCAAT GAGCCTCGTC 
TTCTACGCAA AGCTGCTCTC GATCCTCACC GAGCTGCGTA CGCTGGGCAA CCAGAACGCC 
GAGATGTGTT TCTCACTAAA GCTCAAAAAC CGCAAACTGC CCAAGTTCCT CGAGGAGATC 
TGGGACGTTC ATGCCATCCC GCCATCGGTC CAGTCGCACC TTCAGATTAC CCAGGAGGAG 
AACGAGCGTC TCGAGCGGGC TGAGCGTATG CGGGCATCGG TTGGGGGCGC CATTACCGCC 
GGCATTGATT GCGACTCTGC CTCCACTTCG GCGGCGGCAG CCGCGGCCCA GCATCAGCCT 
CAGCCTCAGC CCCAGCCCCA ACCCTCCTCC CTGACCCAGA ACGATTCCCA GCACCAGACA 
CAGCCGCAGC TACAACCTCA GCTACCACCT CAGCTGCAAG GTCAACTGCA ACCCCAGCTC 
CAACCACAGC TTCAGACGCA ACTCCAGCCA CAGATTCAAC CACAGCCACA GCTCCTTCCC 
GTCTCCGCTC CCGTGCCCGC CTCCGTAACC GCACCTGGTT CCTTGTCCGC GGTCAGTACG 
AGCAGCGAAT ACATGGGCGG AAGTGCGGCC ATAGGACCCA TCACGCCGGC AACCACCAGC 
AGTATCACGG CTGCCGTTAC CGCTAGCTCC ACCACATCAG CGGTACCGAT GGGCAACGGA 
GTTGGAGTCG GTGTTGGGGT GGGCGGCAAC GTCAGCATGT ATGCGAACGC CCAGACGGCG 
ATGGCCTTGA TGGGTGTAGC CCTGCATTCG CACCAAGAGC AGCTTATCGG GGGAGTGGCG 
GTTAAGTCGG AGCACTCGAC GACTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EcR ( NM_165463.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724458.1 Drosophila melanogaster ecdysone receptor, transcript variant E (EcR), mRNA.

Price & Availability↑

CloneID	OFa09009
Clone ID Related Accession (Same CDS sequence)	NM_165461.3 , NM_165463.2 , NM_165462.2
Accession Version	NM_165463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecdysone receptor, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_165463.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACGA CGAGTGGACA ACAACAGTCA AAGCAAAAGC TGTCGACCTT ACCTAGCCAT 
ATTTTGCTAC AACAACAATT AGCTGCCTCT GCAGGCCCAT CTTCCTCAGT ATCCCTATCC 
CCATCTTCTT CTGCCGCCCT GACCCTGCAT GTAGCCAGTG CTAATGGCGG TGCAAGAGAA 
ACAACTTCTG CAGCCGCAGT CAAGGATAAA CTAAGGCCGA CACCCACAGC CATTAAAATT 
GAGCCCATGC CGGACGTCAT TTCCGTAGGC ACGGTGGCCG GAGGATCTAG CGTAGCTACT 
GTTGTTGCTC CCGCAGCAAC CACAACTTCC AATAAGCCAA ATTCTACAGC GGCGCCCTCG 
ACGTCGGCTG CAGCTGCAAA TGGTCATTTG GTGCTTGTGC CGAACAAAAG ACCGCGACTT 
GATGTTACCG AGGACTGGAT GTCCACTCCT AGTCCAGGCA GCGTGCCCAG CTCTGCGCCG 
CCACTTTCGC CTTCCCCCGG CTCGCAGAAT CACAGCTACA ACATGTCCAA CGGCTATGCA 
TCCCCCATGT CCGCCGGAAG CTATGATCCT TACAGTCCTA CCGGCAAAAC AGGTCGCGAT 
GATCTCTCGC CTTCGAGCAG CTTGAACGGA TACTCGGCGA ACGAAAGCTG CGATGCGAAG 
AAGAGCAAGA AGGGACCTGC GCCACGGGTG CAAGAGGAGC TGTGCCTGGT TTGCGGCGAC 
AGGGCCTCCG GCTACCACTA CAACGCCCTC ACCTGTGAGG GCTGCAAGGG GTTCTTTCGA 
CGCAGCGTTA CGAAGAGCGC CGTCTACTGC TGCAAGTTCG GGCGCGCCTG CGAAATGGAC 
ATGTACATGA GGCGAAAGTG TCAGGAGTGC CGCCTGAAAA AGTGCCTGGC CGTGGGTATG 
CGGCCGGAAT GCGTCGTCCC GGAGAACCAA TGTGCGATGA AGCGGCGCGA AAAGAAGGCC 
CAGAAGGAGA AGGACAAAAT GACCACTTCG CCGAGCTCTC AGCATGGCGG CAATGGCAGC 
TTGGCCTCTG GTGGCGGCCA AGACTTTGTT AAGAAGGAGA TTCTTGACCT TATGACATGC 
GAGCCGCCCC AGCATGCCAC TATTCCGCTA CTACCTGATG AAATATTGGC CAAGTGTCAA 
GCGCGCAATA TACCTTCCTT AACGTACAAT CAGTTGGCCG TTATATACAA GTTAATTTGG 
TACCAGGATG GCTATGAGCA GCCATCTGAA GAGGATCTCA GGCGTATAAT GAGTCAACCC 
GATGAGAACG AGAGCCAAAC GGACGTCAGC TTTCGGCATA TAACCGAGAT AACCATACTC 
ACGGTCCAGT TGATTGTTGA GTTTGCTAAA GGTCTACCAG CGTTTACAAA GATACCCCAG 
GAGGACCAGA TCACGTTACT AAAGGCCTGC TCGTCGGAGG TGATGATGCT GCGTATGGCA 
CGACGCTATG ACCACAGCTC GGACTCAATA TTCTTCGCGA ATAATAGATC ATATACGCGG 
GATTCTTACA AAATGGCCGG AATGGCTGAT AACATTGAAG ACCTGCTGCA TTTCTGCCGC 
CAAATGTTCT CGATGAAGGT GGACAACGTC GAATACGCGC TTCTCACTGC CATTGTGATC 
TTCTCGGACC GGCCGGGCCT GGAGAAGGCC CAACTAGTCG AAGCGATCCA GAGCTACTAC 
ATCGACACGC TACGCATTTA TATACTCAAC CGCCACTGCG GCGACTCAAT GAGCCTCGTC 
TTCTACGCAA AGCTGCTCTC GATCCTCACC GAGCTGCGTA CGCTGGGCAA CCAGAACGCC 
GAGATGTGTT TCTCACTAAA GCTCAAAAAC CGCAAACTGC CCAAGTTCCT CGAGGAGATC 
TGGGACGTTC ATGCCATCCC GCCATCGGTC CAGTCGCACC TTCAGATTAC CCAGGAGGAG 
AACGAGCGTC TCGAGCGGGC TGAGCGTATG CGGGCATCGG TTGGGGGCGC CATTACCGCC 
GGCATTGATT GCGACTCTGC CTCCACTTCG GCGGCGGCAG CCGCGGCCCA GCATCAGCCT 
CAGCCTCAGC CCCAGCCCCA ACCCTCCTCC CTGACCCAGA ACGATTCCCA GCACCAGACA 
CAGCCGCAGC TACAACCTCA GCTACCACCT CAGCTGCAAG GTCAACTGCA ACCCCAGCTC 
CAACCACAGC TTCAGACGCA ACTCCAGCCA CAGATTCAAC CACAGCCACA GCTCCTTCCC 
GTCTCCGCTC CCGTGCCCGC CTCCGTAACC GCACCTGGTT CCTTGTCCGC GGTCAGTACG 
AGCAGCGAAT ACATGGGCGG AAGTGCGGCC ATAGGACCCA TCACGCCGGC AACCACCAGC 
AGTATCACGG CTGCCGTTAC CGCTAGCTCC ACCACATCAG CGGTACCGAT GGGCAACGGA 
GTTGGAGTCG GTGTTGGGGT GGGCGGCAAC GTCAGCATGT ATGCGAACGC CCAGACGGCG 
ATGGCCTTGA TGGGTGTAGC CCTGCATTCG CACCAAGAGC AGCTTATCGG GGGAGTGGCG 
GTTAAGTCGG AGCACTCGAC GACTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724458.1
CDS	515..3064
Translation	MLTTSGQQQSKQKLSTLPSHILLQQQLAASAGPSSSVSLSPSSSAALTLHVASANGGARETTSAAAVKDKLRPTPTAIKIEPMPDVISVGTVAGGSSVATVVAPAATTTSNKPNSTAAPSTSAAAANGHLVLVPNKRPRLDVTEDWMSTPSPGSVPSSAPPLSPSPGSQNHSYNMSNGYASPMSAGSYDPYSPTGKTGRDDLSPSSSLNGYSANESCDAKKSKKGPAPRVQEELCLVCGDRASGYHYNALTCEGCKGFFRRSVTKSAVYCCKFGRACEMDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGGNGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIYKLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAFTKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIEDLLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHCGDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQSHLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSLTQNDSQHQTQPQLQPQLPPQLQGQLQPQLQPQLQTQLQPQIQPQPQLLPVSAPVPASVTAPGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGGNVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA

Target ORF information:

RefSeq Version	NM_165463.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecdysone receptor, transcript variant E (EcR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165463.2

ORF Insert Sequence:

ATGTTGACGA CGAGTGGACA ACAACAGTCA AAGCAAAAGC TGTCGACCTT ACCTAGCCAT 
ATTTTGCTAC AACAACAATT AGCTGCCTCT GCAGGCCCAT CTTCCTCAGT ATCCCTATCC 
CCATCTTCTT CTGCCGCCCT GACCCTGCAT GTAGCCAGTG CTAATGGCGG TGCAAGAGAA 
ACAACTTCTG CAGCCGCAGT CAAGGATAAA CTAAGGCCGA CACCCACAGC CATTAAAATT 
GAGCCCATGC CGGACGTCAT TTCCGTAGGC ACGGTGGCCG GAGGATCTAG CGTAGCTACT 
GTTGTTGCTC CCGCAGCAAC CACAACTTCC AATAAGCCAA ATTCTACAGC GGCGCCCTCG 
ACGTCGGCTG CAGCTGCAAA TGGTCATTTG GTGCTTGTGC CGAACAAAAG ACCGCGACTT 
GATGTTACCG AGGACTGGAT GTCCACTCCT AGTCCAGGCA GCGTGCCCAG CTCTGCGCCG 
CCACTTTCGC CTTCCCCCGG CTCGCAGAAT CACAGCTACA ACATGTCCAA CGGCTATGCA 
TCCCCCATGT CCGCCGGAAG CTATGATCCT TACAGTCCTA CCGGCAAAAC AGGTCGCGAT 
GATCTCTCGC CTTCGAGCAG CTTGAACGGA TACTCGGCGA ACGAAAGCTG CGATGCGAAG 
AAGAGCAAGA AGGGACCTGC GCCACGGGTG CAAGAGGAGC TGTGCCTGGT TTGCGGCGAC 
AGGGCCTCCG GCTACCACTA CAACGCCCTC ACCTGTGAGG GCTGCAAGGG GTTCTTTCGA 
CGCAGCGTTA CGAAGAGCGC CGTCTACTGC TGCAAGTTCG GGCGCGCCTG CGAAATGGAC 
ATGTACATGA GGCGAAAGTG TCAGGAGTGC CGCCTGAAAA AGTGCCTGGC CGTGGGTATG 
CGGCCGGAAT GCGTCGTCCC GGAGAACCAA TGTGCGATGA AGCGGCGCGA AAAGAAGGCC 
CAGAAGGAGA AGGACAAAAT GACCACTTCG CCGAGCTCTC AGCATGGCGG CAATGGCAGC 
TTGGCCTCTG GTGGCGGCCA AGACTTTGTT AAGAAGGAGA TTCTTGACCT TATGACATGC 
GAGCCGCCCC AGCATGCCAC TATTCCGCTA CTACCTGATG AAATATTGGC CAAGTGTCAA 
GCGCGCAATA TACCTTCCTT AACGTACAAT CAGTTGGCCG TTATATACAA GTTAATTTGG 
TACCAGGATG GCTATGAGCA GCCATCTGAA GAGGATCTCA GGCGTATAAT GAGTCAACCC 
GATGAGAACG AGAGCCAAAC GGACGTCAGC TTTCGGCATA TAACCGAGAT AACCATACTC 
ACGGTCCAGT TGATTGTTGA GTTTGCTAAA GGTCTACCAG CGTTTACAAA GATACCCCAG 
GAGGACCAGA TCACGTTACT AAAGGCCTGC TCGTCGGAGG TGATGATGCT GCGTATGGCA 
CGACGCTATG ACCACAGCTC GGACTCAATA TTCTTCGCGA ATAATAGATC ATATACGCGG 
GATTCTTACA AAATGGCCGG AATGGCTGAT AACATTGAAG ACCTGCTGCA TTTCTGCCGC 
CAAATGTTCT CGATGAAGGT GGACAACGTC GAATACGCGC TTCTCACTGC CATTGTGATC 
TTCTCGGACC GGCCGGGCCT GGAGAAGGCC CAACTAGTCG AAGCGATCCA GAGCTACTAC 
ATCGACACGC TACGCATTTA TATACTCAAC CGCCACTGCG GCGACTCAAT GAGCCTCGTC 
TTCTACGCAA AGCTGCTCTC GATCCTCACC GAGCTGCGTA CGCTGGGCAA CCAGAACGCC 
GAGATGTGTT TCTCACTAAA GCTCAAAAAC CGCAAACTGC CCAAGTTCCT CGAGGAGATC 
TGGGACGTTC ATGCCATCCC GCCATCGGTC CAGTCGCACC TTCAGATTAC CCAGGAGGAG 
AACGAGCGTC TCGAGCGGGC TGAGCGTATG CGGGCATCGG TTGGGGGCGC CATTACCGCC 
GGCATTGATT GCGACTCTGC CTCCACTTCG GCGGCGGCAG CCGCGGCCCA GCATCAGCCT 
CAGCCTCAGC CCCAGCCCCA ACCCTCCTCC CTGACCCAGA ACGATTCCCA GCACCAGACA 
CAGCCGCAGC TACAACCTCA GCTACCACCT CAGCTGCAAG GTCAACTGCA ACCCCAGCTC 
CAACCACAGC TTCAGACGCA ACTCCAGCCA CAGATTCAAC CACAGCCACA GCTCCTTCCC 
GTCTCCGCTC CCGTGCCCGC CTCCGTAACC GCACCTGGTT CCTTGTCCGC GGTCAGTACG 
AGCAGCGAAT ACATGGGCGG AAGTGCGGCC ATAGGACCCA TCACGCCGGC AACCACCAGC 
AGTATCACGG CTGCCGTTAC CGCTAGCTCC ACCACATCAG CGGTACCGAT GGGCAACGGA 
GTTGGAGTCG GTGTTGGGGT GGGCGGCAAC GTCAGCATGT ATGCGAACGC CCAGACGGCG 
ATGGCCTTGA TGGGTGTAGC CCTGCATTCG CACCAAGAGC AGCTTATCGG GGGAGTGGCG 
GTTAAGTCGG AGCACTCGAC GACTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EcR ( NM_165462.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724457.1 Drosophila melanogaster ecdysone receptor, transcript variant D (EcR), mRNA.

Price & Availability↑

CloneID	OFa09009
Clone ID Related Accession (Same CDS sequence)	NM_165461.3 , NM_165463.2 , NM_165462.2
Accession Version	NM_165462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecdysone receptor, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_165462.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACGA CGAGTGGACA ACAACAGTCA AAGCAAAAGC TGTCGACCTT ACCTAGCCAT 
ATTTTGCTAC AACAACAATT AGCTGCCTCT GCAGGCCCAT CTTCCTCAGT ATCCCTATCC 
CCATCTTCTT CTGCCGCCCT GACCCTGCAT GTAGCCAGTG CTAATGGCGG TGCAAGAGAA 
ACAACTTCTG CAGCCGCAGT CAAGGATAAA CTAAGGCCGA CACCCACAGC CATTAAAATT 
GAGCCCATGC CGGACGTCAT TTCCGTAGGC ACGGTGGCCG GAGGATCTAG CGTAGCTACT 
GTTGTTGCTC CCGCAGCAAC CACAACTTCC AATAAGCCAA ATTCTACAGC GGCGCCCTCG 
ACGTCGGCTG CAGCTGCAAA TGGTCATTTG GTGCTTGTGC CGAACAAAAG ACCGCGACTT 
GATGTTACCG AGGACTGGAT GTCCACTCCT AGTCCAGGCA GCGTGCCCAG CTCTGCGCCG 
CCACTTTCGC CTTCCCCCGG CTCGCAGAAT CACAGCTACA ACATGTCCAA CGGCTATGCA 
TCCCCCATGT CCGCCGGAAG CTATGATCCT TACAGTCCTA CCGGCAAAAC AGGTCGCGAT 
GATCTCTCGC CTTCGAGCAG CTTGAACGGA TACTCGGCGA ACGAAAGCTG CGATGCGAAG 
AAGAGCAAGA AGGGACCTGC GCCACGGGTG CAAGAGGAGC TGTGCCTGGT TTGCGGCGAC 
AGGGCCTCCG GCTACCACTA CAACGCCCTC ACCTGTGAGG GCTGCAAGGG GTTCTTTCGA 
CGCAGCGTTA CGAAGAGCGC CGTCTACTGC TGCAAGTTCG GGCGCGCCTG CGAAATGGAC 
ATGTACATGA GGCGAAAGTG TCAGGAGTGC CGCCTGAAAA AGTGCCTGGC CGTGGGTATG 
CGGCCGGAAT GCGTCGTCCC GGAGAACCAA TGTGCGATGA AGCGGCGCGA AAAGAAGGCC 
CAGAAGGAGA AGGACAAAAT GACCACTTCG CCGAGCTCTC AGCATGGCGG CAATGGCAGC 
TTGGCCTCTG GTGGCGGCCA AGACTTTGTT AAGAAGGAGA TTCTTGACCT TATGACATGC 
GAGCCGCCCC AGCATGCCAC TATTCCGCTA CTACCTGATG AAATATTGGC CAAGTGTCAA 
GCGCGCAATA TACCTTCCTT AACGTACAAT CAGTTGGCCG TTATATACAA GTTAATTTGG 
TACCAGGATG GCTATGAGCA GCCATCTGAA GAGGATCTCA GGCGTATAAT GAGTCAACCC 
GATGAGAACG AGAGCCAAAC GGACGTCAGC TTTCGGCATA TAACCGAGAT AACCATACTC 
ACGGTCCAGT TGATTGTTGA GTTTGCTAAA GGTCTACCAG CGTTTACAAA GATACCCCAG 
GAGGACCAGA TCACGTTACT AAAGGCCTGC TCGTCGGAGG TGATGATGCT GCGTATGGCA 
CGACGCTATG ACCACAGCTC GGACTCAATA TTCTTCGCGA ATAATAGATC ATATACGCGG 
GATTCTTACA AAATGGCCGG AATGGCTGAT AACATTGAAG ACCTGCTGCA TTTCTGCCGC 
CAAATGTTCT CGATGAAGGT GGACAACGTC GAATACGCGC TTCTCACTGC CATTGTGATC 
TTCTCGGACC GGCCGGGCCT GGAGAAGGCC CAACTAGTCG AAGCGATCCA GAGCTACTAC 
ATCGACACGC TACGCATTTA TATACTCAAC CGCCACTGCG GCGACTCAAT GAGCCTCGTC 
TTCTACGCAA AGCTGCTCTC GATCCTCACC GAGCTGCGTA CGCTGGGCAA CCAGAACGCC 
GAGATGTGTT TCTCACTAAA GCTCAAAAAC CGCAAACTGC CCAAGTTCCT CGAGGAGATC 
TGGGACGTTC ATGCCATCCC GCCATCGGTC CAGTCGCACC TTCAGATTAC CCAGGAGGAG 
AACGAGCGTC TCGAGCGGGC TGAGCGTATG CGGGCATCGG TTGGGGGCGC CATTACCGCC 
GGCATTGATT GCGACTCTGC CTCCACTTCG GCGGCGGCAG CCGCGGCCCA GCATCAGCCT 
CAGCCTCAGC CCCAGCCCCA ACCCTCCTCC CTGACCCAGA ACGATTCCCA GCACCAGACA 
CAGCCGCAGC TACAACCTCA GCTACCACCT CAGCTGCAAG GTCAACTGCA ACCCCAGCTC 
CAACCACAGC TTCAGACGCA ACTCCAGCCA CAGATTCAAC CACAGCCACA GCTCCTTCCC 
GTCTCCGCTC CCGTGCCCGC CTCCGTAACC GCACCTGGTT CCTTGTCCGC GGTCAGTACG 
AGCAGCGAAT ACATGGGCGG AAGTGCGGCC ATAGGACCCA TCACGCCGGC AACCACCAGC 
AGTATCACGG CTGCCGTTAC CGCTAGCTCC ACCACATCAG CGGTACCGAT GGGCAACGGA 
GTTGGAGTCG GTGTTGGGGT GGGCGGCAAC GTCAGCATGT ATGCGAACGC CCAGACGGCG 
ATGGCCTTGA TGGGTGTAGC CCTGCATTCG CACCAAGAGC AGCTTATCGG GGGAGTGGCG 
GTTAAGTCGG AGCACTCGAC GACTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724457.1
CDS	469..3018
Translation	MLTTSGQQQSKQKLSTLPSHILLQQQLAASAGPSSSVSLSPSSSAALTLHVASANGGARETTSAAAVKDKLRPTPTAIKIEPMPDVISVGTVAGGSSVATVVAPAATTTSNKPNSTAAPSTSAAAANGHLVLVPNKRPRLDVTEDWMSTPSPGSVPSSAPPLSPSPGSQNHSYNMSNGYASPMSAGSYDPYSPTGKTGRDDLSPSSSLNGYSANESCDAKKSKKGPAPRVQEELCLVCGDRASGYHYNALTCEGCKGFFRRSVTKSAVYCCKFGRACEMDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGGNGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIYKLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAFTKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIEDLLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHCGDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQSHLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSLTQNDSQHQTQPQLQPQLPPQLQGQLQPQLQPQLQTQLQPQIQPQPQLLPVSAPVPASVTAPGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGGNVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA

Target ORF information:

RefSeq Version	NM_165462.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecdysone receptor, transcript variant D (EcR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165462.2

ORF Insert Sequence:

ATGTTGACGA CGAGTGGACA ACAACAGTCA AAGCAAAAGC TGTCGACCTT ACCTAGCCAT 
ATTTTGCTAC AACAACAATT AGCTGCCTCT GCAGGCCCAT CTTCCTCAGT ATCCCTATCC 
CCATCTTCTT CTGCCGCCCT GACCCTGCAT GTAGCCAGTG CTAATGGCGG TGCAAGAGAA 
ACAACTTCTG CAGCCGCAGT CAAGGATAAA CTAAGGCCGA CACCCACAGC CATTAAAATT 
GAGCCCATGC CGGACGTCAT TTCCGTAGGC ACGGTGGCCG GAGGATCTAG CGTAGCTACT 
GTTGTTGCTC CCGCAGCAAC CACAACTTCC AATAAGCCAA ATTCTACAGC GGCGCCCTCG 
ACGTCGGCTG CAGCTGCAAA TGGTCATTTG GTGCTTGTGC CGAACAAAAG ACCGCGACTT 
GATGTTACCG AGGACTGGAT GTCCACTCCT AGTCCAGGCA GCGTGCCCAG CTCTGCGCCG 
CCACTTTCGC CTTCCCCCGG CTCGCAGAAT CACAGCTACA ACATGTCCAA CGGCTATGCA 
TCCCCCATGT CCGCCGGAAG CTATGATCCT TACAGTCCTA CCGGCAAAAC AGGTCGCGAT 
GATCTCTCGC CTTCGAGCAG CTTGAACGGA TACTCGGCGA ACGAAAGCTG CGATGCGAAG 
AAGAGCAAGA AGGGACCTGC GCCACGGGTG CAAGAGGAGC TGTGCCTGGT TTGCGGCGAC 
AGGGCCTCCG GCTACCACTA CAACGCCCTC ACCTGTGAGG GCTGCAAGGG GTTCTTTCGA 
CGCAGCGTTA CGAAGAGCGC CGTCTACTGC TGCAAGTTCG GGCGCGCCTG CGAAATGGAC 
ATGTACATGA GGCGAAAGTG TCAGGAGTGC CGCCTGAAAA AGTGCCTGGC CGTGGGTATG 
CGGCCGGAAT GCGTCGTCCC GGAGAACCAA TGTGCGATGA AGCGGCGCGA AAAGAAGGCC 
CAGAAGGAGA AGGACAAAAT GACCACTTCG CCGAGCTCTC AGCATGGCGG CAATGGCAGC 
TTGGCCTCTG GTGGCGGCCA AGACTTTGTT AAGAAGGAGA TTCTTGACCT TATGACATGC 
GAGCCGCCCC AGCATGCCAC TATTCCGCTA CTACCTGATG AAATATTGGC CAAGTGTCAA 
GCGCGCAATA TACCTTCCTT AACGTACAAT CAGTTGGCCG TTATATACAA GTTAATTTGG 
TACCAGGATG GCTATGAGCA GCCATCTGAA GAGGATCTCA GGCGTATAAT GAGTCAACCC 
GATGAGAACG AGAGCCAAAC GGACGTCAGC TTTCGGCATA TAACCGAGAT AACCATACTC 
ACGGTCCAGT TGATTGTTGA GTTTGCTAAA GGTCTACCAG CGTTTACAAA GATACCCCAG 
GAGGACCAGA TCACGTTACT AAAGGCCTGC TCGTCGGAGG TGATGATGCT GCGTATGGCA 
CGACGCTATG ACCACAGCTC GGACTCAATA TTCTTCGCGA ATAATAGATC ATATACGCGG 
GATTCTTACA AAATGGCCGG AATGGCTGAT AACATTGAAG ACCTGCTGCA TTTCTGCCGC 
CAAATGTTCT CGATGAAGGT GGACAACGTC GAATACGCGC TTCTCACTGC CATTGTGATC 
TTCTCGGACC GGCCGGGCCT GGAGAAGGCC CAACTAGTCG AAGCGATCCA GAGCTACTAC 
ATCGACACGC TACGCATTTA TATACTCAAC CGCCACTGCG GCGACTCAAT GAGCCTCGTC 
TTCTACGCAA AGCTGCTCTC GATCCTCACC GAGCTGCGTA CGCTGGGCAA CCAGAACGCC 
GAGATGTGTT TCTCACTAAA GCTCAAAAAC CGCAAACTGC CCAAGTTCCT CGAGGAGATC 
TGGGACGTTC ATGCCATCCC GCCATCGGTC CAGTCGCACC TTCAGATTAC CCAGGAGGAG 
AACGAGCGTC TCGAGCGGGC TGAGCGTATG CGGGCATCGG TTGGGGGCGC CATTACCGCC 
GGCATTGATT GCGACTCTGC CTCCACTTCG GCGGCGGCAG CCGCGGCCCA GCATCAGCCT 
CAGCCTCAGC CCCAGCCCCA ACCCTCCTCC CTGACCCAGA ACGATTCCCA GCACCAGACA 
CAGCCGCAGC TACAACCTCA GCTACCACCT CAGCTGCAAG GTCAACTGCA ACCCCAGCTC 
CAACCACAGC TTCAGACGCA ACTCCAGCCA CAGATTCAAC CACAGCCACA GCTCCTTCCC 
GTCTCCGCTC CCGTGCCCGC CTCCGTAACC GCACCTGGTT CCTTGTCCGC GGTCAGTACG 
AGCAGCGAAT ACATGGGCGG AAGTGCGGCC ATAGGACCCA TCACGCCGGC AACCACCAGC 
AGTATCACGG CTGCCGTTAC CGCTAGCTCC ACCACATCAG CGGTACCGAT GGGCAACGGA 
GTTGGAGTCG GTGTTGGGGT GGGCGGCAAC GTCAGCATGT ATGCGAACGC CCAGACGGCG 
ATGGCCTTGA TGGGTGTAGC CCTGCATTCG CACCAAGAGC AGCTTATCGG GGGAGTGGCG 
GTTAAGTCGG AGCACTCGAC GACTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EcR ( NM_165464.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724459.1 Drosophila melanogaster ecdysone receptor, transcript variant C (EcR), mRNA.

Price & Availability↑

CloneID	OFa09010
Clone ID Related Accession (Same CDS sequence)	NM_165464.3
Accession Version	NM_165464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecdysone receptor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165464.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATACTT GTGGATTAGT AGCAGAACTG GCGCACTATA TCGACGCATA TGGTCGCGAT 
GATCTCTCGC CTTCGAGCAG CTTGAACGGA TACTCGGCGA ACGAAAGCTG CGATGCGAAG 
AAGAGCAAGA AGGGACCTGC GCCACGGGTG CAAGAGGAGC TGTGCCTGGT TTGCGGCGAC 
AGGGCCTCCG GCTACCACTA CAACGCCCTC ACCTGTGAGG GCTGCAAGGG GTTCTTTCGA 
CGCAGCGTTA CGAAGAGCGC CGTCTACTGC TGCAAGTTCG GGCGCGCCTG CGAAATGGAC 
ATGTACATGA GGCGAAAGTG TCAGGAGTGC CGCCTGAAAA AGTGCCTGGC CGTGGGTATG 
CGGCCGGAAT GCGTCGTCCC GGAGAACCAA TGTGCGATGA AGCGGCGCGA AAAGAAGGCC 
CAGAAGGAGA AGGACAAAAT GACCACTTCG CCGAGCTCTC AGCATGGCGG CAATGGCAGC 
TTGGCCTCTG GTGGCGGCCA AGACTTTGTT AAGAAGGAGA TTCTTGACCT TATGACATGC 
GAGCCGCCCC AGCATGCCAC TATTCCGCTA CTACCTGATG AAATATTGGC CAAGTGTCAA 
GCGCGCAATA TACCTTCCTT AACGTACAAT CAGTTGGCCG TTATATACAA GTTAATTTGG 
TACCAGGATG GCTATGAGCA GCCATCTGAA GAGGATCTCA GGCGTATAAT GAGTCAACCC 
GATGAGAACG AGAGCCAAAC GGACGTCAGC TTTCGGCATA TAACCGAGAT AACCATACTC 
ACGGTCCAGT TGATTGTTGA GTTTGCTAAA GGTCTACCAG CGTTTACAAA GATACCCCAG 
GAGGACCAGA TCACGTTACT AAAGGCCTGC TCGTCGGAGG TGATGATGCT GCGTATGGCA 
CGACGCTATG ACCACAGCTC GGACTCAATA TTCTTCGCGA ATAATAGATC ATATACGCGG 
GATTCTTACA AAATGGCCGG AATGGCTGAT AACATTGAAG ACCTGCTGCA TTTCTGCCGC 
CAAATGTTCT CGATGAAGGT GGACAACGTC GAATACGCGC TTCTCACTGC CATTGTGATC 
TTCTCGGACC GGCCGGGCCT GGAGAAGGCC CAACTAGTCG AAGCGATCCA GAGCTACTAC 
ATCGACACGC TACGCATTTA TATACTCAAC CGCCACTGCG GCGACTCAAT GAGCCTCGTC 
TTCTACGCAA AGCTGCTCTC GATCCTCACC GAGCTGCGTA CGCTGGGCAA CCAGAACGCC 
GAGATGTGTT TCTCACTAAA GCTCAAAAAC CGCAAACTGC CCAAGTTCCT CGAGGAGATC 
TGGGACGTTC ATGCCATCCC GCCATCGGTC CAGTCGCACC TTCAGATTAC CCAGGAGGAG 
AACGAGCGTC TCGAGCGGGC TGAGCGTATG CGGGCATCGG TTGGGGGCGC CATTACCGCC 
GGCATTGATT GCGACTCTGC CTCCACTTCG GCGGCGGCAG CCGCGGCCCA GCATCAGCCT 
CAGCCTCAGC CCCAGCCCCA ACCCTCCTCC CTGACCCAGA ACGATTCCCA GCACCAGACA 
CAGCCGCAGC TACAACCTCA GCTACCACCT CAGCTGCAAG GTCAACTGCA ACCCCAGCTC 
CAACCACAGC TTCAGACGCA ACTCCAGCCA CAGATTCAAC CACAGCCACA GCTCCTTCCC 
GTCTCCGCTC CCGTGCCCGC CTCCGTAACC GCACCTGGTT CCTTGTCCGC GGTCAGTACG 
AGCAGCGAAT ACATGGGCGG AAGTGCGGCC ATAGGACCCA TCACGCCGGC AACCACCAGC 
AGTATCACGG CTGCCGTTAC CGCTAGCTCC ACCACATCAG CGGTACCGAT GGGCAACGGA 
GTTGGAGTCG GTGTTGGGGT GGGCGGCAAC GTCAGCATGT ATGCGAACGC CCAGACGGCG 
ATGGCCTTGA TGGGTGTAGC CCTGCATTCG CACCAAGAGC AGCTTATCGG GGGAGTGGCG 
GTTAAGTCGG AGCACTCGAC GACTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724459.1
CDS	806..2815
Translation	MDTCGLVAELAHYIDAYGRDDLSPSSSLNGYSANESCDAKKSKKGPAPRVQEELCLVCGDRASGYHYNALTCEGCKGFFRRSVTKSAVYCCKFGRACEMDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGGNGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIYKLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAFTKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIEDLLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHCGDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQSHLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSLTQNDSQHQTQPQLQPQLPPQLQGQLQPQLQPQLQTQLQPQIQPQPQLLPVSAPVPASVTAPGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGGNVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA

Target ORF information:

RefSeq Version	NM_165464.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecdysone receptor, transcript variant C (EcR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165464.3

ORF Insert Sequence:

ATGGATACTT GTGGATTAGT AGCAGAACTG GCGCACTATA TCGACGCATA TGGTCGCGAT 
GATCTCTCGC CTTCGAGCAG CTTGAACGGA TACTCGGCGA ACGAAAGCTG CGATGCGAAG 
AAGAGCAAGA AGGGACCTGC GCCACGGGTG CAAGAGGAGC TGTGCCTGGT TTGCGGCGAC 
AGGGCCTCCG GCTACCACTA CAACGCCCTC ACCTGTGAGG GCTGCAAGGG GTTCTTTCGA 
CGCAGCGTTA CGAAGAGCGC CGTCTACTGC TGCAAGTTCG GGCGCGCCTG CGAAATGGAC 
ATGTACATGA GGCGAAAGTG TCAGGAGTGC CGCCTGAAAA AGTGCCTGGC CGTGGGTATG 
CGGCCGGAAT GCGTCGTCCC GGAGAACCAA TGTGCGATGA AGCGGCGCGA AAAGAAGGCC 
CAGAAGGAGA AGGACAAAAT GACCACTTCG CCGAGCTCTC AGCATGGCGG CAATGGCAGC 
TTGGCCTCTG GTGGCGGCCA AGACTTTGTT AAGAAGGAGA TTCTTGACCT TATGACATGC 
GAGCCGCCCC AGCATGCCAC TATTCCGCTA CTACCTGATG AAATATTGGC CAAGTGTCAA 
GCGCGCAATA TACCTTCCTT AACGTACAAT CAGTTGGCCG TTATATACAA GTTAATTTGG 
TACCAGGATG GCTATGAGCA GCCATCTGAA GAGGATCTCA GGCGTATAAT GAGTCAACCC 
GATGAGAACG AGAGCCAAAC GGACGTCAGC TTTCGGCATA TAACCGAGAT AACCATACTC 
ACGGTCCAGT TGATTGTTGA GTTTGCTAAA GGTCTACCAG CGTTTACAAA GATACCCCAG 
GAGGACCAGA TCACGTTACT AAAGGCCTGC TCGTCGGAGG TGATGATGCT GCGTATGGCA 
CGACGCTATG ACCACAGCTC GGACTCAATA TTCTTCGCGA ATAATAGATC ATATACGCGG 
GATTCTTACA AAATGGCCGG AATGGCTGAT AACATTGAAG ACCTGCTGCA TTTCTGCCGC 
CAAATGTTCT CGATGAAGGT GGACAACGTC GAATACGCGC TTCTCACTGC CATTGTGATC 
TTCTCGGACC GGCCGGGCCT GGAGAAGGCC CAACTAGTCG AAGCGATCCA GAGCTACTAC 
ATCGACACGC TACGCATTTA TATACTCAAC CGCCACTGCG GCGACTCAAT GAGCCTCGTC 
TTCTACGCAA AGCTGCTCTC GATCCTCACC GAGCTGCGTA CGCTGGGCAA CCAGAACGCC 
GAGATGTGTT TCTCACTAAA GCTCAAAAAC CGCAAACTGC CCAAGTTCCT CGAGGAGATC 
TGGGACGTTC ATGCCATCCC GCCATCGGTC CAGTCGCACC TTCAGATTAC CCAGGAGGAG 
AACGAGCGTC TCGAGCGGGC TGAGCGTATG CGGGCATCGG TTGGGGGCGC CATTACCGCC 
GGCATTGATT GCGACTCTGC CTCCACTTCG GCGGCGGCAG CCGCGGCCCA GCATCAGCCT 
CAGCCTCAGC CCCAGCCCCA ACCCTCCTCC CTGACCCAGA ACGATTCCCA GCACCAGACA 
CAGCCGCAGC TACAACCTCA GCTACCACCT CAGCTGCAAG GTCAACTGCA ACCCCAGCTC 
CAACCACAGC TTCAGACGCA ACTCCAGCCA CAGATTCAAC CACAGCCACA GCTCCTTCCC 
GTCTCCGCTC CCGTGCCCGC CTCCGTAACC GCACCTGGTT CCTTGTCCGC GGTCAGTACG 
AGCAGCGAAT ACATGGGCGG AAGTGCGGCC ATAGGACCCA TCACGCCGGC AACCACCAGC 
AGTATCACGG CTGCCGTTAC CGCTAGCTCC ACCACATCAG CGGTACCGAT GGGCAACGGA 
GTTGGAGTCG GTGTTGGGGT GGGCGGCAAC GTCAGCATGT ATGCGAACGC CCAGACGGCG 
ATGGCCTTGA TGGGTGTAGC CCTGCATTCG CACCAAGAGC AGCTTATCGG GGGAGTGGCG 
GTTAAGTCGG AGCACTCGAC GACTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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EcR cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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