Act42A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Act42A
Entrez Gene ID	35526
Full Name	Actin 42A
Synonyms	42A,A,ACT2_DROME,AFFX-Dros-ACTIN_M_r_at,Act,Act42,Act42a,Actin,Actin/BAP47,ArpB,BAP47,C,CG12051,Dmel\CG12051,act 42A,act42A,actin
General protein information	Preferred Names Actin 42A Names CG12051 gene product from transcript CG12051-RA 42A actin Act42A-PA Actin-42A CG12051-PA F-actin actin actin 42A actin42a
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42A7-42A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_078901.3	NP_523625.1	actin 42A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	POTEI	XP_001143715.1
Caenorhabditis elegans (roundworm)	act-5	NP_499809.1
Homo sapiens (human)	POTEI	NP_001264335.1
Rattus norvegicus (Norway rat)	Potef	XP_002728578.1
Gallus gallus (chicken)	LOC776816	XP_001236316.2
Arabidopsis thaliana (thale cress)	ACT11	NP_187818.1
Drosophila melanogaster (fruit fly)	Act42A	NP_523625.1
Oryza sativa (rice)	Os05g0106600	NP_001054419.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings show an interdependency between Hpo signaling activity and F-actin dynamics in which CP and Hpo pathway activities inhibit F-actin accumulation, and the reduction in F-actin in turn sustains Hpo pathway activity
Title: Actin-Capping Protein and the Hippo pathway regulate F-actin and tissue growth in Drosophila.

Data raise the possibility that the Drosophila embryo is divided into zones of different PTB and Notch activities based on whether or not they are linked to actin regulation.
Title: Loss of PTB or negative regulation of Notch mRNA reveals distinct zones of Notch and actin protein accumulation in Drosophila embryo.

MOESIN crosslinks this protein and cell membrane in Drosophila oocytes and is required for OSKAR anchoring.
Title: MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring.

essential cytoplasmic actin isoform
Title: One of the two cytoplasmic actin isoforms in Drosophila is essential.

The following Act42A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Act42A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12416	NM_078901.3 Latest version!	Drosophila melanogaster actin 42A (Act42A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Act42A ( NM_078901.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523625.1 Drosophila melanogaster actin 42A (Act42A), mRNA.

Price & Availability↑

CloneID	OFa12416
Clone ID Related Accession (Same CDS sequence)	NM_078901.3
Accession Version	NM_078901.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	actin 42A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_078901.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGACG AAGAGGTTGC AGCTTTAGTG GTCGACAACG GATCCGGCAT GTGCAAAGCC 
GGCTTTGCCG GTGATGACGC ACCGCGTGCA GTTTTTCCTT CTATTGTCGG CCGTCCACGT 
CACCAGGGCG TAATGGTAGG AATGGGACAA AAGGACTCTT ATGTCGGCGA TGAGGCACAG 
AGCAAACGTG GTATCCTTAC CCTGAAGTAC CCCATTGAGC ACGGTATCGT GACTAACTGG 
GACGACATGG AGAAGATCTG GCATCACACT TTCTACAACG AGCTTCGTGT GGCCCCGGAG 
GAGCACCCCG TCTTGCTTAC TGAGGCTCCT TTGAACCCCA AGGCTAATCG CGAGAAGATG 
ACTCAGATTA TGTTTGAAAC CTTCAACACT CCGGCCATGT ATGTTGCCAT CCAGGCGGTG 
CTTTCTCTCT ACGCCTCCGG CCGTACCACA GGTATCGTGT TGGACTCCGG CGACGGTGTC 
TCCCATACTG TGCCCATTTA TGAGGGCTAC GCCCTGCCGC ACGCCATCCT CCGTTTGGAT 
CTAGCCGGTC GCGATTTAAC CGACTACCTG ATGAAGATTC TTACTGAGCG CGGTTACAGC 
TTCACCACCA CCGCCGAGCG TGAAATTGTG CGCGACATCA AGGAGAAGCT GTGCTACGTG 
GCCTTGGACT TCGAGCAGGA GATGGCCACG GCCGCTTCAA GCTCGTCCCT GGAGAAGTCG 
TACGAGTTGC CCGATGGACA GGTCATCACC ATCGGAAATG AGCGATTCCG TTGCCCCGAA 
TCGCTGTTCC AGCCGTCGTT CCTCGGCATG GAGGCCTGTG GCATTCACGA GACCACCTAC 
AACTCAATCA TGAAGTGTGA CGTCGACATC CGTAAGGATC TGTACGCCAA CACTGTGCTG 
TCCGGCGGCA CCACCATGTA CCCGGGAATC GCTGACCGCA TGCAAAAGGA AATCACGGCG 
TTGGCTCCGT CCACCATGAA GATTAAGATT GTTGCCCCGC CAGAACGCAA GTACTCTGTT 
TGGATCGGCG GCTCCATCCT GGCTTCGCTG TCTACTTTCC AGCAGATGTG GATCTCGAAG 
CAAGAGTACG ACGAGTCGGG CCCCTCCATT GTTCACCGCA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523625.1
CDS	136..1266
Translation	MCDEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPESLFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_078901.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster actin 42A (Act42A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078901.3

ORF Insert Sequence:

ATGTGTGACG AAGAGGTTGC AGCTTTAGTG GTCGACAACG GATCCGGCAT GTGCAAAGCC 
GGCTTTGCCG GTGATGACGC ACCGCGTGCA GTTTTTCCTT CTATTGTCGG CCGTCCACGT 
CACCAGGGCG TAATGGTAGG AATGGGACAA AAGGACTCTT ATGTCGGCGA TGAGGCACAG 
AGCAAACGTG GTATCCTTAC CCTGAAGTAC CCCATTGAGC ACGGTATCGT GACTAACTGG 
GACGACATGG AGAAGATCTG GCATCACACT TTCTACAACG AGCTTCGTGT GGCCCCGGAG 
GAGCACCCCG TCTTGCTTAC TGAGGCTCCT TTGAACCCCA AGGCTAATCG CGAGAAGATG 
ACTCAGATTA TGTTTGAAAC CTTCAACACT CCGGCCATGT ATGTTGCCAT CCAGGCGGTG 
CTTTCTCTCT ACGCCTCCGG CCGTACCACA GGTATCGTGT TGGACTCCGG CGACGGTGTC 
TCCCATACTG TGCCCATTTA TGAGGGCTAC GCCCTGCCGC ACGCCATCCT CCGTTTGGAT 
CTAGCCGGTC GCGATTTAAC CGACTACCTG ATGAAGATTC TTACTGAGCG CGGTTACAGC 
TTCACCACCA CCGCCGAGCG TGAAATTGTG CGCGACATCA AGGAGAAGCT GTGCTACGTG 
GCCTTGGACT TCGAGCAGGA GATGGCCACG GCCGCTTCAA GCTCGTCCCT GGAGAAGTCG 
TACGAGTTGC CCGATGGACA GGTCATCACC ATCGGAAATG AGCGATTCCG TTGCCCCGAA 
TCGCTGTTCC AGCCGTCGTT CCTCGGCATG GAGGCCTGTG GCATTCACGA GACCACCTAC 
AACTCAATCA TGAAGTGTGA CGTCGACATC CGTAAGGATC TGTACGCCAA CACTGTGCTG 
TCCGGCGGCA CCACCATGTA CCCGGGAATC GCTGACCGCA TGCAAAAGGA AATCACGGCG 
TTGGCTCCGT CCACCATGAA GATTAAGATT GTTGCCCCGC CAGAACGCAA GTACTCTGTT 
TGGATCGGCG GCTCCATCCT GGCTTCGCTG TCTACTTTCC AGCAGATGTG GATCTCGAAG 
CAAGAGTACG ACGAGTCGGG CCCCTCCATT GTTCACCGCA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Act42A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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