Src42A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Src42A
Entrez Gene ID	35524
Full Name	Src oncogene at 42A
Synonyms	CG14471,CG44128,CG7873,DSrc42A,Dm SRC41,DmHD-29,Dmel\CG44128,Dmel_CG14471,Dmel_CG7873,Dsrc41,Dsrc42A,Dtk5,HD-29,SK2-4,SRC 42A,SRC42A,Src,Src41,Src42,Src42a,Su(D-raf)1,Su(Raf)1,Su(phl)1,Su1,Tk5,dSrc,dtk-5,dtk5,l(2)k10108,src,src42,src42A,src42a
General protein information	Preferred Names Src oncogene at 42A Names CG44128 gene product from transcript CG44128-RA CG44128-PA CG44128-PB Src42A-PA Src42A-PB src oncogene at 42A suppressor of pole hole suppressor of raf-like-oncogene tyrosine kinase 5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42A6-42A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_136347.2	NP_610191.2	Src oncogene at 42A, isoform B
NM_057501.4	NP_476849.1	Src oncogene at 42A, isoform A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FRK	XP_002690130.1
Gallus gallus (chicken)	FRK	XP_419779.3
Caenorhabditis elegans (roundworm)	src-2	NP_493502.1
Xenopus tropicalis (tropical clawed frog)	frk	XP_002938349.1
Canis lupus familiaris (dog)	FRK	XP_539091.2
Rattus norvegicus (Norway rat)	Frk	NP_077344.1
Pan troglodytes (chimpanzee)	FRK	XP_518702.2
	FRK	XP_001112190.1
Danio rerio (zebrafish)	LOC567549	XP_695937.5
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006270	XP_316335.3
Homo sapiens (human)	FRK	NP_002022.1
Drosophila melanogaster (fruit fly)	Src42A	NP_476849.1
Mus musculus (house mouse)	Frk	NP_034367.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Significantly, the JNK pathway is responsible for the majority of the phenotypes and transcriptional changes downstream of Notch-Src synergy.
Title: The Notch-mediated hyperplasia circuitry in Drosophila reveals a Src-JNK signaling axis.

show the same signaling occurs in Drosophila inflammatory cells in response to wound-induced H2O2 with mutants for the Lyn homolog Src42A displaying impaired inflammatory migration to wounds
Title: Draper/CED-1 mediates an ancient damage response to control inflammatory blood cell migration in vivo.

Results show that Src42a and Src64b are required for the normal division capacity of Drosophila intestinal progenitor cells, and that these genes as well as Ack cause progenitor overproliferation in the intestine via core cell cycle activation.
Title: Src kinase function controls progenitor cell pools during regeneration and tumor onset in the Drosophila intestine.

Reduction of Src42A function leads to nuclear translocation and activation of FOXO proteins.
Title: Src tyrosine kinase signaling antagonizes nuclear localization of FOXO and inhibits its transcription factor activity.

the Bendless (Ben)/dUev1a ubiquitin E2 complex is an essential regulator of Src42A-induced, JNK-mediated cell migration.
Title: Src42A modulates tumor invasion and cell death via Ben/dUev1a-mediated JNK activation in Drosophila.

The following Src42A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Src42A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12414	NM_057501.4 Latest version!	Drosophila melanogaster Src oncogene at 42A (Src42A), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12415	NM_136347.2 Latest version!	Drosophila melanogaster Src oncogene at 42A (Src42A), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Src42A ( NM_057501.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476849.1 Drosophila melanogaster Src oncogene at 42A (Src42A), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12414
Clone ID Related Accession (Same CDS sequence)	NM_057501.4
Accession Version	NM_057501.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Src oncogene at 42A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057501.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAACT GCCTCACCAC ACAGAAGGGC GAACCCGACA AGCCCGCAGA TCGAATCAAG 
CTGGACGACC CGCCCACCAT CGGAGTCGGA GTGGGCGTGC CACAAATCCC CATGCCCTCA 
CACGCCGGAC AGCCACCGGA GCAGATACGT CCGGTTCCCC AGATCCCGGA GAGCGAAACG 
GCAGGTGCCA ACGCCAAGAT TTTTGTCGCC CTCTACGACT ACGACGCCCG CACCGACGAG 
GATTTGAGCT TCCGCAAGGG AGAGCACTTG GAGATACTGA ATGACACGCA GGGTGACTGG 
TGGCTGGCGC GGAGCAAGAA GACACGTTCG GAAGGCTACA TTCCATCCAA TTATGTGGCC 
AAGTTGAAAT CAATCGAAGC AGAACCCTGG TACTTCCGCA AAATCAAACG CATTGAGGCT 
GAGAAAAAAC TTCTACTGCC AGAGAACGAG CACGGTGCAT TTTTAATTCG CGATTCCGAA 
AGCCGTCACA ACGACTACTC GCTATCAGTG CGCGATGGCG ATACGGTTAA GCATTATCGC 
ATCAGACAAT TGGACGAAGG CGGCTTCTTC ATCGCCAGGC GCACGACATT CAGAACCCTT 
CAGGAGCTGG TGGAACACTA TTCGAAGGAC TCTGATGGCC TATGCGTCAA CCTCTGCAAG 
CCGTGTGTCC AGATCGAGAA GCCTGTAACT GAGGGGCTTT CGCACCGCAC TCGCGATCAG 
TGGGAGATCG ACAGAACGTC TTTGAAATTC GTGCGCAAAC TGGGCTCCGG ACAGTTTGGC 
GATGTCTGGG AGGGATTGTG GAACAACACA ACACCTGTGG CAATTAAAAC TCTGAAATCT 
GGTACAATGG ACCCCAAGGA TTTCTTAGCG GAAGCCCAGA TCATGAAGAA ACTGCGCCAC 
ACCAAGCTTA TACAGTTGTA CGCTGTCTGC ACTGTTGAGG AGCCTATCTA TATTATCACA 
GAGTTAATGA AGCACGGTTC ACTGTTGGAA TATCTCCAAG CCATTGCAGG CAAGGGTCGT 
AGCCTTAAAA TGCAAACTCT GATTGATATG GCAGCGCAAA TAGCTGCTGG CATGGCTTAC 
TTGGAGTCCC AGAATTATAT TCATAGGGAT TTAGCGGCGC GCAATGTACT GGTAGGCGAT 
GGAAACATCG TCAAAATCGC CGACTTTGGT TTAGCTAGGC TCATCAAGGA GGACGAATAC 
GAGGCGCGGG TAGGCGCCAG ATTTCCCATA AAATGGACCG CTCCAGAGGC TGCTAACTAC 
AGCAAATTCT CAATAAAATC GGATGTTTGG AGCTTTGGCA TTCTTCTCAC AGAACTGGTC 
ACCTACGGAC GCATACCATA TCCAGGCATG ACCAACGCTG AGGTGCTAAC GCAAGTGGAG 
CACGGCTATC GAATGCCGCA ACCTCCCAAC TGCGAGCCGC GCCTGTATGA GATTATGCTG 
GAATGTTGGC ACAAGGACCC CATGCGCAGA CCCACGTTTG AGACGCTACA ATGGAAACTG 
GAAGACTTCT ATACATCTGA TCAGAGCGAC TACAAAGAGG CGCAGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476849.1
CDS	575..2128
Translation	MGNCLTTQKGEPDKPADRIKLDDPPTIGVGVGVPQIPMPSHAGQPPEQIRPVPQIPESETAGANAKIFVALYDYDARTDEDLSFRKGEHLEILNDTQGDWWLARSKKTRSEGYIPSNYVAKLKSIEAEPWYFRKIKRIEAEKKLLLPENEHGAFLIRDSESRHNDYSLSVRDGDTVKHYRIRQLDEGGFFIARRTTFRTLQELVEHYSKDSDGLCVNLCKPCVQIEKPVTEGLSHRTRDQWEIDRTSLKFVRKLGSGQFGDVWEGLWNNTTPVAIKTLKSGTMDPKDFLAEAQIMKKLRHTKLIQLYAVCTVEEPIYIITELMKHGSLLEYLQAIAGKGRSLKMQTLIDMAAQIAAGMAYLESQNYIHRDLAARNVLVGDGNIVKIADFGLARLIKEDEYEARVGARFPIKWTAPEAANYSKFSIKSDVWSFGILLTELVTYGRIPYPGMTNAEVLTQVEHGYRMPQPPNCEPRLYEIMLECWHKDPMRRPTFETLQWKLEDFYTSDQSDYKEAQAY

Target ORF information:

RefSeq Version	NM_057501.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Src oncogene at 42A (Src42A), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057501.4

ORF Insert Sequence:

ATGGGTAACT GCCTCACCAC ACAGAAGGGC GAACCCGACA AGCCCGCAGA TCGAATCAAG 
CTGGACGACC CGCCCACCAT CGGAGTCGGA GTGGGCGTGC CACAAATCCC CATGCCCTCA 
CACGCCGGAC AGCCACCGGA GCAGATACGT CCGGTTCCCC AGATCCCGGA GAGCGAAACG 
GCAGGTGCCA ACGCCAAGAT TTTTGTCGCC CTCTACGACT ACGACGCCCG CACCGACGAG 
GATTTGAGCT TCCGCAAGGG AGAGCACTTG GAGATACTGA ATGACACGCA GGGTGACTGG 
TGGCTGGCGC GGAGCAAGAA GACACGTTCG GAAGGCTACA TTCCATCCAA TTATGTGGCC 
AAGTTGAAAT CAATCGAAGC AGAACCCTGG TACTTCCGCA AAATCAAACG CATTGAGGCT 
GAGAAAAAAC TTCTACTGCC AGAGAACGAG CACGGTGCAT TTTTAATTCG CGATTCCGAA 
AGCCGTCACA ACGACTACTC GCTATCAGTG CGCGATGGCG ATACGGTTAA GCATTATCGC 
ATCAGACAAT TGGACGAAGG CGGCTTCTTC ATCGCCAGGC GCACGACATT CAGAACCCTT 
CAGGAGCTGG TGGAACACTA TTCGAAGGAC TCTGATGGCC TATGCGTCAA CCTCTGCAAG 
CCGTGTGTCC AGATCGAGAA GCCTGTAACT GAGGGGCTTT CGCACCGCAC TCGCGATCAG 
TGGGAGATCG ACAGAACGTC TTTGAAATTC GTGCGCAAAC TGGGCTCCGG ACAGTTTGGC 
GATGTCTGGG AGGGATTGTG GAACAACACA ACACCTGTGG CAATTAAAAC TCTGAAATCT 
GGTACAATGG ACCCCAAGGA TTTCTTAGCG GAAGCCCAGA TCATGAAGAA ACTGCGCCAC 
ACCAAGCTTA TACAGTTGTA CGCTGTCTGC ACTGTTGAGG AGCCTATCTA TATTATCACA 
GAGTTAATGA AGCACGGTTC ACTGTTGGAA TATCTCCAAG CCATTGCAGG CAAGGGTCGT 
AGCCTTAAAA TGCAAACTCT GATTGATATG GCAGCGCAAA TAGCTGCTGG CATGGCTTAC 
TTGGAGTCCC AGAATTATAT TCATAGGGAT TTAGCGGCGC GCAATGTACT GGTAGGCGAT 
GGAAACATCG TCAAAATCGC CGACTTTGGT TTAGCTAGGC TCATCAAGGA GGACGAATAC 
GAGGCGCGGG TAGGCGCCAG ATTTCCCATA AAATGGACCG CTCCAGAGGC TGCTAACTAC 
AGCAAATTCT CAATAAAATC GGATGTTTGG AGCTTTGGCA TTCTTCTCAC AGAACTGGTC 
ACCTACGGAC GCATACCATA TCCAGGCATG ACCAACGCTG AGGTGCTAAC GCAAGTGGAG 
CACGGCTATC GAATGCCGCA ACCTCCCAAC TGCGAGCCGC GCCTGTATGA GATTATGCTG 
GAATGTTGGC ACAAGGACCC CATGCGCAGA CCCACGTTTG AGACGCTACA ATGGAAACTG 
GAAGACTTCT ATACATCTGA TCAGAGCGAC TACAAAGAGG CGCAGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Src42A ( NM_136347.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610191.2 Drosophila melanogaster Src oncogene at 42A (Src42A), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12415
Clone ID Related Accession (Same CDS sequence)	NM_136347.2
Accession Version	NM_136347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Src oncogene at 42A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136347.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGCCA TGCCCGTGGT GTGGTGCACC GATCCCAACT CGGTGAACAA TAAGCCCACA 
GCCTTCGCCT TGCACGGAAT TCAGCTGCAA GGAAATGTAA CCAAGAAACA GGTCCATGCC 
CTCCGGGAGC TGGTAAATGC CGCCGCTGAG GGCCGCCTCA TCCTCCCCTC GGACGGTACT 
TTTACTCTGT TGGACACTGG GCTTAGCATT GAAAGCTCTA TCGTAGAGCA GGAGTCAAAC 
AACTCTAATA GTCCTGGTGA GCCTAGTACA TTGGCTCAAC CAAAGAATTC CAAGAACACC 
TATATCCTGA TGCCCGTCAG CAAGGCAGAT GGATCGAGAC AAGCAGGTGT AGTGAACGGA 
GGTCTTGACT CGTCCGTACA ATCGATTGCA GCCGCCGCCG CGCAACTGGA GTTCCTGCTC 
GATCCCAAAT ACTCGTCCAC CCCTGAAGCC AGCCCAGATG TTGAACCAAG TGTAGAACTG 
GCTGAGGGTG GGGCGGCAGG AGAGATTTTG TACGAGTTCC TGTGCTGCGC CAAGTGCATG 
AACGAACAGC GGACCGGACT CACTTGCGCT GGACGTCGCC TGCAGCGCTA TTTAAGGACA 
TGCAGCAGTG GAAGTGGCAA CGCCAGTGGT AGAAACCGCG ACTTCGAGAC GATCAACGAT 
CCAGCTACAT GGCAGCTGAA ACTTGATCCT GTCAGCAGCA TCGCCAGCAA GTACGCCCGC 
CGACAGCAGA GACAGAGGCA GCATGGCACC GTTTCCATGG GCATCGCACG TAATCGCACC 
CAACGCCATC ACAAATTACC CATGCACAGT GTTAAAATCT TTCATAATCG CAGCACTTAC 
ATGCCAGCTA CTACAGCAAC AGGAGCACTA GCAATAGCTG GGGGGGGCCC AGCTTGCCTT 
GTGGGCGGAG GCCGGGAGGT GTCATCAATT CCGATGCCGA TTCCAAGTCC GAGCTCGAAT 
CCAAATTCCA AACCCACCCC AGCGAATAGA CCGATCGTCG GCAGTGAAGC GTTTAGTTTG 
TTACCGACAA CGGCGAGATC TACATCGTTG ACGGAAACAG CACTCGCGAA AACGGCAGCA 
ACAGTTCTAC CTCTCTACGC TGTTGTTAGT AAAAATCGTG AATTAGAGAG CATTACAAAG 
ACTGTGGAGG TCCCAACCTC AGCTATGCTA GCCGTCGTGC TTGAGGAGAC GTCTTCACAT 
ACTGCAGATG GACGCGATCT GATCAGTTTT GACGACGACC AGGCGCAGGT GTGTGCTGAG 
GCGGGGTATG TAAGCTACCA GGCCGGAAGT CCCGTGGATC TTCTGTGCGC CCCAAATGAA 
GAAAACACAC TGGTGGAGAC TCCGCCGGCC ACTTCGGCCA AGCCCTGTAT ACCCGGAAGC 
AACAATTCAA GCCGAAAAAC CAGCTTTGAT TCTACCAGCA CCCTCAGTTC CATGGACTCT 
GGTTTCATGG AGATGCAGAA CAAATTGGAT GCCTTGGCAG CAGCAGCAGC AGCAACACTA 
ATAGGCGAAT CAACACCACC TTTAGGCGAA GCAGCAGCAC CTTTAGGCGA AGCTGCACCA 
TCGTCGGCGA TCGGCGAGAA AATTGACCGA CGGAACTACA AGGAATGTCT GACGCAGTCA 
CGAAATCGCC GAAAATCCTA TGAGGAATTC AAAGCAATGT TTATTGAACC CCCCGCGGAA 
GCATCCAAAT CAGTTACCGT GCCACCGCCG GTGGTAGCAC CAGCGGATGA CGCAACAACA 
GCCAGCAGCA CTGTGATCCC ACTTTCCTCC ATTTCAGAAC AAGAAAATAT AGGCAGCCGG 
GGTGTAAGGG GCAAACCGGA TTGCGCAAAC GAGTTTACCG CCAGCACTGA TGAAATGGAT 
ACCGGCGATG GTAACAGCGA TGGTGAAAAA GGCGCTGCTG CCGAAGTCGA TCTGACAACA 
GAAACAACAG CAACAGCAAA AACTTTAGTG AGCAGCTCCA CCACAGAGGC GATGCGAAAG 
AATTCCGATT TTCTATCGCA GATTCTCGAT CATCAATTTG CGGCCAAGGA GCGAGCCAAG 
GCGCACAAAC GTCGCACTTC ATACGAGGAA TTTAAGCGAC TGGTTAACGA AATTGAGCCG 
CTGGAATGCC CGAACACCCA GCCTACCGTC TCGGCTGCGG TCACACCCAC AACCGCAACA 
GCAGCAGCAG CAGCATCTCT GATAAAACGC CAAAACTCGC GGCAGCGTAA AAGCTTTGCC 
AGCTACTTTT TGCCGCACCG GCATTCGACC AAAGAACAAA AGACTGAAAA GGAAAACTGC 
TCTCGGGATC CAATAAAGCC TTCACAGGAG CAACAGCTGG CGGTCTGTGG CAAGCTTCCG 
CTTCACAGGA GCAACACCAG CGACATCTCT AGCTCCTACT ATCGGCGCAA TTTCAAGATC 
TACGATAAAC TGGTCTACGG TACTATCTAC GATATCATCC AGCGCAAAAA TGACATCTAC 
CAAAAATACG ACAAGTACAT GACATATGGC ACCATATATG AGATTCTTCA CCGCAAGACC 
TCGCAAAGCA GCGATCCGTG GCAACGTAAA AGCCTTAGCT CCATTCTGGA GGGAGGGCGA 
TCTTCGGGCG ACATCATCTA TGACATCGGT CAGAGGCGGG AACGTGATAA GCGCACCCTG 
GGCTCCTCGT CTTCGGCCAC TGTCTCCACG ATCAATCCTC ACAAATATGG AACGATTTAT 
GATATACTGC AGGGCGTTAA AATAGATGCG GGTCATGTCA AAAATAAACC GGACCAAAAA 
AGTAAACCGA CCAAGCCACC ACTGGCCAAG CCAACGCGCT TCGTGGTCAG CCAGGTGGTA 
GAGCCCGTCC AGATACCGCC CACGGATCAG TCGGGAAAGA GTGTCCAGGA GCAAGGAGCA 
CCAAAATCTA ACAAACCAAA CAAAATGCGC CGTCTATCTA ACATCCTAAG CTATAGTCGC 
AACTCTGGCG AGGATCAGCA GGAAACGACT GGAAAGGAGG AGACTAAACC AAAACGCACT 
CAGGCCAAGC GACGGATCGG AGTCACCAAC TTGTTGTTGC CCCTGGATTC TGAGGAGCTC 
TACACTCGCA TTATAGCCCA GCAGCGGGGG CTTGATAAGG AGCACAGTCA GGAACCAGAA 
GAGAAGTGCG CCAGCGAGGA GCAGATGTGC ACCCGCAACG CGTTGACCAA GTCTAGCTCA 
CTAGACGCCA TCTCACTTTC AGTAGCCATT TCGCCTGCGT CGTCGCCATC CCCTCCGCGT 
CCGCGCCGTA GTCTTAAGCA ACACCGCTGC CTCCAGCAAC ACCAGCAAAC GCCACTTGCC 
AAAAAGCACT CTCTGGACAA CTGCCTTCAA GTAGTGTCGT CGACTACTAG CCGGCAGCCA 
CTTCGTCGGT GGTCTAACCA GACTCCTCTG AAGTGCACCT GCCACCCCGC AACCGAGGAA 
CTTCCGCTGA CCGCCATAGA GAAGTCCCGC TCGCTCCCCG CCGCCTGTTC CGCCTCCTGC 
TCTCATACGT GTGCACACTT AAGTGTAATC GATCAAAGCC CAGCTACAAC AAAGTGCTTC 
GGAAAAACAT CATTGACGAC GGCCAAGAAA GGCAAATCGC GTCGTTTATC AGAATTTACG 
CGCGGTGAAT TTTTAAATGA AAAATCCTGG TACTTCCGCA AAATCAAACG CATTGAGGCT 
GAGAAAAAAC TTCTACTGCC AGAGAACGAG CACGGTGCAT TTTTAATTCG CGATTCCGAA 
AGCCGTCACA ACGACTACTC GCTATCAGTG CGCGATGGCG ATACGGTTAA GCATTATCGC 
ATCAGACAAT TGGACGAAGG CGGCTTCTTC ATCGCCAGGC GCACGACATT CAGAACCCTT 
CAGGAGCTGG TGGAACACTA TTCGAAGGAC TCTGATGGCC TATGCGTCAA CCTCTGCAAG 
CCGTGTGTCC AGATCGAGAA GCCTGTAACT GAGGGGCTTT CGCACCGCAC TCGCGATCAG 
TGGGAGATCG ACAGAACGTC TTTGAAATTC GTGCGCAAAC TGGGCTCCGG ACAGTTTGGC 
GATGTCTGGG AGGGATTGTG GAACAACACA ACACCTGTGG CAATTAAAAC TCTGAAATCT 
GGTACAATGG ACCCCAAGGA TTTCTTAGCG GAAGCCCAGA TCATGAAGAA ACTGCGCCAC 
ACCAAGCTTA TACAGTTGTA CGCTGTCTGC ACTGTTGAGG AGCCTATCTA TATTATCACA 
GAGTTAATGA AGCACGGTTC ACTGTTGGAA TATCTCCAAG CCATTGCAGG CAAGGGTCGT 
AGCCTTAAAA TGCAAACTCT GATTGATATG GCAGCGCAAA TAGCTGCTGG CATGGCTTAC 
TTGGAGTCCC AGAATTATAT TCATAGGGAT TTAGCGGCGC GCAATGTACT GGTAGGCGAT 
GGAAACATCG TCAAAATCGC CGACTTTGGT TTAGCTAGGC TCATCAAGGA GGACGAATAC 
GAGGCGCGGG TAGGCGCCAG ATTTCCCATA AAATGGACCG CTCCAGAGGC TGCTAACTAC 
AGCAAATTCT CAATAAAATC GGATGTTTGG AGCTTTGGCA TTCTTCTCAC AGAACTGGTC 
ACCTACGGAC GCATACCATA TCCAGGCATG ACCAACGCTG AGGTGCTAAC GCAAGTGGAG 
CACGGCTATC GAATGCCGCA ACCTCCCAAC TGCGAGCCGC GCCTGTATGA GATTATGCTG 
GAATGTTGGC ACAAGGACCC CATGCGCAGA CCCACGTTTG AGACGCTACA ATGGAAACTG 
GAAGACTTCT ATACATCTGA TCAGAGCGAC TACAAAGAGG CGCAGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610191.2
CDS	421..5214
Translation	MTAMPVVWCTDPNSVNNKPTAFALHGIQLQGNVTKKQVHALRELVNAAAEGRLILPSDGTFTLLDTGLSIESSIVEQESNNSNSPGEPSTLAQPKNSKNTYILMPVSKADGSRQAGVVNGGLDSSVQSIAAAAAQLEFLLDPKYSSTPEASPDVEPSVELAEGGAAGEILYEFLCCAKCMNEQRTGLTCAGRRLQRYLRTCSSGSGNASGRNRDFETINDPATWQLKLDPVSSIASKYARRQQRQRQHGTVSMGIARNRTQRHHKLPMHSVKIFHNRSTYMPATTATGALAIAGGGPACLVGGGREVSSIPMPIPSPSSNPNSKPTPANRPIVGSEAFSLLPTTARSTSLTETALAKTAATVLPLYAVVSKNRELESITKTVEVPTSAMLAVVLEETSSHTADGRDLISFDDDQAQVCAEAGYVSYQAGSPVDLLCAPNEENTLVETPPATSAKPCIPGSNNSSRKTSFDSTSTLSSMDSGFMEMQNKLDALAAAAAATLIGESTPPLGEAAAPLGEAAPSSAIGEKIDRRNYKECLTQSRNRRKSYEEFKAMFIEPPAEASKSVTVPPPVVAPADDATTASSTVIPLSSISEQENIGSRGVRGKPDCANEFTASTDEMDTGDGNSDGEKGAAAEVDLTTETTATAKTLVSSSTTEAMRKNSDFLSQILDHQFAAKERAKAHKRRTSYEEFKRLVNEIEPLECPNTQPTVSAAVTPTTATAAAAASLIKRQNSRQRKSFASYFLPHRHSTKEQKTEKENCSRDPIKPSQEQQLAVCGKLPLHRSNTSDISSSYYRRNFKIYDKLVYGTIYDIIQRKNDIYQKYDKYMTYGTIYEILHRKTSQSSDPWQRKSLSSILEGGRSSGDIIYDIGQRRERDKRTLGSSSSATVSTINPHKYGTIYDILQGVKIDAGHVKNKPDQKSKPTKPPLAKPTRFVVSQVVEPVQIPPTDQSGKSVQEQGAPKSNKPNKMRRLSNILSYSRNSGEDQQETTGKEETKPKRTQAKRRIGVTNLLLPLDSEELYTRIIAQQRGLDKEHSQEPEEKCASEEQMCTRNALTKSSSLDAISLSVAISPASSPSPPRPRRSLKQHRCLQQHQQTPLAKKHSLDNCLQVVSSTTSRQPLRRWSNQTPLKCTCHPATEELPLTAIEKSRSLPAACSASCSHTCAHLSVIDQSPATTKCFGKTSLTTAKKGKSRRLSEFTRGEFLNEKSWYFRKIKRIEAEKKLLLPENEHGAFLIRDSESRHNDYSLSVRDGDTVKHYRIRQLDEGGFFIARRTTFRTLQELVEHYSKDSDGLCVNLCKPCVQIEKPVTEGLSHRTRDQWEIDRTSLKFVRKLGSGQFGDVWEGLWNNTTPVAIKTLKSGTMDPKDFLAEAQIMKKLRHTKLIQLYAVCTVEEPIYIITELMKHGSLLEYLQAIAGKGRSLKMQTLIDMAAQIAAGMAYLESQNYIHRDLAARNVLVGDGNIVKIADFGLARLIKEDEYEARVGARFPIKWTAPEAANYSKFSIKSDVWSFGILLTELVTYGRIPYPGMTNAEVLTQVEHGYRMPQPPNCEPRLYEIMLECWHKDPMRRPTFETLQWKLEDFYTSDQSDYKEAQAY

Target ORF information:

RefSeq Version	NM_136347.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Src oncogene at 42A (Src42A), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136347.2

ORF Insert Sequence:

ATGACGGCCA TGCCCGTGGT GTGGTGCACC GATCCCAACT CGGTGAACAA TAAGCCCACA 
GCCTTCGCCT TGCACGGAAT TCAGCTGCAA GGAAATGTAA CCAAGAAACA GGTCCATGCC 
CTCCGGGAGC TGGTAAATGC CGCCGCTGAG GGCCGCCTCA TCCTCCCCTC GGACGGTACT 
TTTACTCTGT TGGACACTGG GCTTAGCATT GAAAGCTCTA TCGTAGAGCA GGAGTCAAAC 
AACTCTAATA GTCCTGGTGA GCCTAGTACA TTGGCTCAAC CAAAGAATTC CAAGAACACC 
TATATCCTGA TGCCCGTCAG CAAGGCAGAT GGATCGAGAC AAGCAGGTGT AGTGAACGGA 
GGTCTTGACT CGTCCGTACA ATCGATTGCA GCCGCCGCCG CGCAACTGGA GTTCCTGCTC 
GATCCCAAAT ACTCGTCCAC CCCTGAAGCC AGCCCAGATG TTGAACCAAG TGTAGAACTG 
GCTGAGGGTG GGGCGGCAGG AGAGATTTTG TACGAGTTCC TGTGCTGCGC CAAGTGCATG 
AACGAACAGC GGACCGGACT CACTTGCGCT GGACGTCGCC TGCAGCGCTA TTTAAGGACA 
TGCAGCAGTG GAAGTGGCAA CGCCAGTGGT AGAAACCGCG ACTTCGAGAC GATCAACGAT 
CCAGCTACAT GGCAGCTGAA ACTTGATCCT GTCAGCAGCA TCGCCAGCAA GTACGCCCGC 
CGACAGCAGA GACAGAGGCA GCATGGCACC GTTTCCATGG GCATCGCACG TAATCGCACC 
CAACGCCATC ACAAATTACC CATGCACAGT GTTAAAATCT TTCATAATCG CAGCACTTAC 
ATGCCAGCTA CTACAGCAAC AGGAGCACTA GCAATAGCTG GGGGGGGCCC AGCTTGCCTT 
GTGGGCGGAG GCCGGGAGGT GTCATCAATT CCGATGCCGA TTCCAAGTCC GAGCTCGAAT 
CCAAATTCCA AACCCACCCC AGCGAATAGA CCGATCGTCG GCAGTGAAGC GTTTAGTTTG 
TTACCGACAA CGGCGAGATC TACATCGTTG ACGGAAACAG CACTCGCGAA AACGGCAGCA 
ACAGTTCTAC CTCTCTACGC TGTTGTTAGT AAAAATCGTG AATTAGAGAG CATTACAAAG 
ACTGTGGAGG TCCCAACCTC AGCTATGCTA GCCGTCGTGC TTGAGGAGAC GTCTTCACAT 
ACTGCAGATG GACGCGATCT GATCAGTTTT GACGACGACC AGGCGCAGGT GTGTGCTGAG 
GCGGGGTATG TAAGCTACCA GGCCGGAAGT CCCGTGGATC TTCTGTGCGC CCCAAATGAA 
GAAAACACAC TGGTGGAGAC TCCGCCGGCC ACTTCGGCCA AGCCCTGTAT ACCCGGAAGC 
AACAATTCAA GCCGAAAAAC CAGCTTTGAT TCTACCAGCA CCCTCAGTTC CATGGACTCT 
GGTTTCATGG AGATGCAGAA CAAATTGGAT GCCTTGGCAG CAGCAGCAGC AGCAACACTA 
ATAGGCGAAT CAACACCACC TTTAGGCGAA GCAGCAGCAC CTTTAGGCGA AGCTGCACCA 
TCGTCGGCGA TCGGCGAGAA AATTGACCGA CGGAACTACA AGGAATGTCT GACGCAGTCA 
CGAAATCGCC GAAAATCCTA TGAGGAATTC AAAGCAATGT TTATTGAACC CCCCGCGGAA 
GCATCCAAAT CAGTTACCGT GCCACCGCCG GTGGTAGCAC CAGCGGATGA CGCAACAACA 
GCCAGCAGCA CTGTGATCCC ACTTTCCTCC ATTTCAGAAC AAGAAAATAT AGGCAGCCGG 
GGTGTAAGGG GCAAACCGGA TTGCGCAAAC GAGTTTACCG CCAGCACTGA TGAAATGGAT 
ACCGGCGATG GTAACAGCGA TGGTGAAAAA GGCGCTGCTG CCGAAGTCGA TCTGACAACA 
GAAACAACAG CAACAGCAAA AACTTTAGTG AGCAGCTCCA CCACAGAGGC GATGCGAAAG 
AATTCCGATT TTCTATCGCA GATTCTCGAT CATCAATTTG CGGCCAAGGA GCGAGCCAAG 
GCGCACAAAC GTCGCACTTC ATACGAGGAA TTTAAGCGAC TGGTTAACGA AATTGAGCCG 
CTGGAATGCC CGAACACCCA GCCTACCGTC TCGGCTGCGG TCACACCCAC AACCGCAACA 
GCAGCAGCAG CAGCATCTCT GATAAAACGC CAAAACTCGC GGCAGCGTAA AAGCTTTGCC 
AGCTACTTTT TGCCGCACCG GCATTCGACC AAAGAACAAA AGACTGAAAA GGAAAACTGC 
TCTCGGGATC CAATAAAGCC TTCACAGGAG CAACAGCTGG CGGTCTGTGG CAAGCTTCCG 
CTTCACAGGA GCAACACCAG CGACATCTCT AGCTCCTACT ATCGGCGCAA TTTCAAGATC 
TACGATAAAC TGGTCTACGG TACTATCTAC GATATCATCC AGCGCAAAAA TGACATCTAC 
CAAAAATACG ACAAGTACAT GACATATGGC ACCATATATG AGATTCTTCA CCGCAAGACC 
TCGCAAAGCA GCGATCCGTG GCAACGTAAA AGCCTTAGCT CCATTCTGGA GGGAGGGCGA 
TCTTCGGGCG ACATCATCTA TGACATCGGT CAGAGGCGGG AACGTGATAA GCGCACCCTG 
GGCTCCTCGT CTTCGGCCAC TGTCTCCACG ATCAATCCTC ACAAATATGG AACGATTTAT 
GATATACTGC AGGGCGTTAA AATAGATGCG GGTCATGTCA AAAATAAACC GGACCAAAAA 
AGTAAACCGA CCAAGCCACC ACTGGCCAAG CCAACGCGCT TCGTGGTCAG CCAGGTGGTA 
GAGCCCGTCC AGATACCGCC CACGGATCAG TCGGGAAAGA GTGTCCAGGA GCAAGGAGCA 
CCAAAATCTA ACAAACCAAA CAAAATGCGC CGTCTATCTA ACATCCTAAG CTATAGTCGC 
AACTCTGGCG AGGATCAGCA GGAAACGACT GGAAAGGAGG AGACTAAACC AAAACGCACT 
CAGGCCAAGC GACGGATCGG AGTCACCAAC TTGTTGTTGC CCCTGGATTC TGAGGAGCTC 
TACACTCGCA TTATAGCCCA GCAGCGGGGG CTTGATAAGG AGCACAGTCA GGAACCAGAA 
GAGAAGTGCG CCAGCGAGGA GCAGATGTGC ACCCGCAACG CGTTGACCAA GTCTAGCTCA 
CTAGACGCCA TCTCACTTTC AGTAGCCATT TCGCCTGCGT CGTCGCCATC CCCTCCGCGT 
CCGCGCCGTA GTCTTAAGCA ACACCGCTGC CTCCAGCAAC ACCAGCAAAC GCCACTTGCC 
AAAAAGCACT CTCTGGACAA CTGCCTTCAA GTAGTGTCGT CGACTACTAG CCGGCAGCCA 
CTTCGTCGGT GGTCTAACCA GACTCCTCTG AAGTGCACCT GCCACCCCGC AACCGAGGAA 
CTTCCGCTGA CCGCCATAGA GAAGTCCCGC TCGCTCCCCG CCGCCTGTTC CGCCTCCTGC 
TCTCATACGT GTGCACACTT AAGTGTAATC GATCAAAGCC CAGCTACAAC AAAGTGCTTC 
GGAAAAACAT CATTGACGAC GGCCAAGAAA GGCAAATCGC GTCGTTTATC AGAATTTACG 
CGCGGTGAAT TTTTAAATGA AAAATCCTGG TACTTCCGCA AAATCAAACG CATTGAGGCT 
GAGAAAAAAC TTCTACTGCC AGAGAACGAG CACGGTGCAT TTTTAATTCG CGATTCCGAA 
AGCCGTCACA ACGACTACTC GCTATCAGTG CGCGATGGCG ATACGGTTAA GCATTATCGC 
ATCAGACAAT TGGACGAAGG CGGCTTCTTC ATCGCCAGGC GCACGACATT CAGAACCCTT 
CAGGAGCTGG TGGAACACTA TTCGAAGGAC TCTGATGGCC TATGCGTCAA CCTCTGCAAG 
CCGTGTGTCC AGATCGAGAA GCCTGTAACT GAGGGGCTTT CGCACCGCAC TCGCGATCAG 
TGGGAGATCG ACAGAACGTC TTTGAAATTC GTGCGCAAAC TGGGCTCCGG ACAGTTTGGC 
GATGTCTGGG AGGGATTGTG GAACAACACA ACACCTGTGG CAATTAAAAC TCTGAAATCT 
GGTACAATGG ACCCCAAGGA TTTCTTAGCG GAAGCCCAGA TCATGAAGAA ACTGCGCCAC 
ACCAAGCTTA TACAGTTGTA CGCTGTCTGC ACTGTTGAGG AGCCTATCTA TATTATCACA 
GAGTTAATGA AGCACGGTTC ACTGTTGGAA TATCTCCAAG CCATTGCAGG CAAGGGTCGT 
AGCCTTAAAA TGCAAACTCT GATTGATATG GCAGCGCAAA TAGCTGCTGG CATGGCTTAC 
TTGGAGTCCC AGAATTATAT TCATAGGGAT TTAGCGGCGC GCAATGTACT GGTAGGCGAT 
GGAAACATCG TCAAAATCGC CGACTTTGGT TTAGCTAGGC TCATCAAGGA GGACGAATAC 
GAGGCGCGGG TAGGCGCCAG ATTTCCCATA AAATGGACCG CTCCAGAGGC TGCTAACTAC 
AGCAAATTCT CAATAAAATC GGATGTTTGG AGCTTTGGCA TTCTTCTCAC AGAACTGGTC 
ACCTACGGAC GCATACCATA TCCAGGCATG ACCAACGCTG AGGTGCTAAC GCAAGTGGAG 
CACGGCTATC GAATGCCGCA ACCTCCCAAC TGCGAGCCGC GCCTGTATGA GATTATGCTG 
GAATGTTGGC ACAAGGACCC CATGCGCAGA CCCACGTTTG AGACGCTACA ATGGAAACTG 
GAAGACTTCT ATACATCTGA TCAGAGCGAC TACAAAGAGG CGCAGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Src42A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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