tj cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tj
Entrez Gene ID	35227
Full Name	traffic jam
Synonyms	CG10034,DM13,Dmaf,Dmel\CG10034,PL3,TJ,Tj,fs(2)eo-B,fs(2)eo2,fs(2)eoPL3,maf-L,maf1
General protein information	Preferred Names traffic jam Names CG10034 gene product from transcript CG10034-RC CG10034-PB CG10034-PC Traffic-Jam female sterile(2)eo2 tj-PB tj-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37E3-37E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_136125.3	NP_609969.2	traffic jam, isoform B
NM_001273653.1	NP_001260582.1	traffic jam, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	tj	NP_001260582.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010030	XP_001689176.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tj regulates the germline stem cell carrying capacity of the niche by controlling the fate and the spatial arrangement of niche cells.
Title: Specification and spatial arrangement of cells in the germline stem cell niche of the Drosophila ovary depend on the Maf transcription factor Traffic jam.

A branched pathway downstream of Notch in which Bowl functions to direct hub cell assembly in parallel to Tj downregulation, is reported.
Title: Traffic jam functions in a branched pathway from Notch activation to niche cell fate.

Data conclude that the regulatory feedback loop between Tj and Slbo is necessary for achieving the correct activity levels of migration-regulating factors to ensure proper BCC motility.
Title: The Maf factor Traffic jam both enables and inhibits collective cell migration in Drosophila oogenesis.

in gonadal somatic cells, tj gives rise simultaneously to two different molecules: the TJ protein, which activates Piwi expression, and piRNAs, which define the Piwi targets for silencing
Title: A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila.

role as critical modulator of the adhesive properties of somatic cells, facilitating germline-soma interactions that are essential for germ cell differentiation
Title: The large Maf factor Traffic Jam controls gonad morphogenesis in Drosophila.

The following tj gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tj cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14972	NM_136125.3 Latest version!	Drosophila melanogaster traffic jam (tj), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16887	NM_001273653.1 Latest version!	Drosophila melanogaster traffic jam (tj), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tj ( NM_136125.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609969.2 Drosophila melanogaster traffic jam (tj), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14972
Clone ID Related Accession (Same CDS sequence)	NM_136125.3
Accession Version	NM_136125.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	traffic jam, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136125.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG AAGATCCCAC CATAAACGAC ACGTACGTTC AGGAGTTTGA TCTGCACCAC 
CTGGAGGTGG GCGGAAATGG AGCTGGCCAA TCGCATGGAG CCGGTGCCGC GTCCCAGGTC 
ACGACTTTGG GCCATGTGAA GCGCGAGGAT CACAGTCCAC CGCAGCCGGT GGCGGTTAAA 
TGGACGACAA TCCACGGCGA TCCCACGAAT CCGGACGATG ACCCGGACTC GCTGCCGCTG 
TCCGGACCAG AGCCCGCCGG AGCAGTGGAG GTCTCGCCAT CGCCGCACAT CAAGCTGAGG 
TCCTTCTCCG GCCATCACCA GTGGCACATG GACGAACGGC GCCTGCAACC GCTCTCTCCG 
CCACCAGAGC AGTATGGTCC GCTGCCCGGA CAGGCGATTC TGGTGAACAC ATCTTCGGGA 
GCGGGCGTTC CGGGCGGAGT GCCCTCAACG CCGCCGGAAA CGCCACCAGT AGTGGGCTCG 
CCCACGGGCA GCAGCAGCTG TCCGGCTCAG ACTTACGCAC ACCACTATGC GAGGACACCG 
GGCAGCTCCG TATCCGTATC CGCCTCCGCC GCCGCCGCAG CTGCTGCCTC CGCCGGAGCA 
GCATCGGTGA GTGCCGGACT GAGCCATGAC ATGATGTGGC TGACCAACTC GATACGGGCG 
GACCAGCAGC CGCTGGATCT GCGGCCTTTG CCCTATCCGG GCTCCCAGGA GGAGGCGGAG 
GAGTGGGACA GACAGCGGGA CTACGCCCTG CAGGCGGCGG CAGCTCATCA CCACCATCAC 
GGACAGCCGC TGATGCAGGC GCAGCATCAT CCGCATGGAC ACGGCGGTCA TCCCCACCAG 
GTGATGCTGC AGCAGAGCAA GTATCCGCAC CACCATCACC ACCACTTCCA CAATCTGGAA 
CTGACGCCCA TCAACATGCA CTCGAACTCG TACGGTAGTG CTTCGGGTGC CCTGACGCCC 
ACCTGTCTGC CCCAAGTGCC CAGCAACGGG AGTGGCAGCA GCGGAGGCGG AGGAGGCAGT 
GGAAGCGGGA GTGGTGGACC CGGCAGCGTG GGTGGCGGCT CCTGCGTCAT CACACGGGCT 
GCCCTTCAGC CGTGCCGCCC GCTCTCCGCC AGCTCGACCA GATCCTCGAA CAACATGTCG 
CCCAGAACCT GTTCGGGTGC CTACAGTAAT GCCACGCTGG AGGATTGCCT CAATGACGAC 
ATGCTGACCA CGTTGACGGT GCGGGAGCTG AACAAGCGGC TCCATGGTTG CCCACGGGAG 
GAGGTAGTGC GTCTGAAGCA GAAGCGACGC ACCCTGAAGA ATCGCGGCTA TGCCCAGAAC 
TGCCGGTCCA AGCGACTCCA TCAGCGCCAC GAACTGGAGA AGGCCAACCG GGTGCTCAAC 
CAGGATCTGC ACCGCCTGAA GCTGGAGTAC TCGAGGGTCT GCCAGGAGCG GGATGCCCTC 
ATGCAGCGTC TGCAGCGGGC GGCAAATGGC GGAGTCGGTG CGGGAGGTGC AACCGCCGGC 
GGCGATAGCC AAAGCTCTCC GGAATTCTAC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609969.2
CDS	490..2025
Translation	MKMEDPTINDTYVQEFDLHHLEVGGNGAGQSHGAGAASQVTTLGHVKREDHSPPQPVAVKWTTIHGDPTNPDDDPDSLPLSGPEPAGAVEVSPSPHIKLRSFSGHHQWHMDERRLQPLSPPPEQYGPLPGQAILVNTSSGAGVPGGVPSTPPETPPVVGSPTGSSSCPAQTYAHHYARTPGSSVSVSASAAAAAAASAGAASVSAGLSHDMMWLTNSIRADQQPLDLRPLPYPGSQEEAEEWDRQRDYALQAAAAHHHHHGQPLMQAQHHPHGHGGHPHQVMLQQSKYPHHHHHHFHNLELTPINMHSNSYGSASGALTPTCLPQVPSNGSGSSGGGGGSGSGSGGPGSVGGGSCVITRAALQPCRPLSASSTRSSNNMSPRTCSGAYSNATLEDCLNDDMLTTLTVRELNKRLHGCPREEVVRLKQKRRTLKNRGYAQNCRSKRLHQRHELEKANRVLNQDLHRLKLEYSRVCQERDALMQRLQRAANGGVGAGGATAGGDSQSSPEFYL

Target ORF information:

RefSeq Version	NM_136125.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster traffic jam (tj), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136125.3

ORF Insert Sequence:

ATGAAAATGG AAGATCCCAC CATAAACGAC ACGTACGTTC AGGAGTTTGA TCTGCACCAC 
CTGGAGGTGG GCGGAAATGG AGCTGGCCAA TCGCATGGAG CCGGTGCCGC GTCCCAGGTC 
ACGACTTTGG GCCATGTGAA GCGCGAGGAT CACAGTCCAC CGCAGCCGGT GGCGGTTAAA 
TGGACGACAA TCCACGGCGA TCCCACGAAT CCGGACGATG ACCCGGACTC GCTGCCGCTG 
TCCGGACCAG AGCCCGCCGG AGCAGTGGAG GTCTCGCCAT CGCCGCACAT CAAGCTGAGG 
TCCTTCTCCG GCCATCACCA GTGGCACATG GACGAACGGC GCCTGCAACC GCTCTCTCCG 
CCACCAGAGC AGTATGGTCC GCTGCCCGGA CAGGCGATTC TGGTGAACAC ATCTTCGGGA 
GCGGGCGTTC CGGGCGGAGT GCCCTCAACG CCGCCGGAAA CGCCACCAGT AGTGGGCTCG 
CCCACGGGCA GCAGCAGCTG TCCGGCTCAG ACTTACGCAC ACCACTATGC GAGGACACCG 
GGCAGCTCCG TATCCGTATC CGCCTCCGCC GCCGCCGCAG CTGCTGCCTC CGCCGGAGCA 
GCATCGGTGA GTGCCGGACT GAGCCATGAC ATGATGTGGC TGACCAACTC GATACGGGCG 
GACCAGCAGC CGCTGGATCT GCGGCCTTTG CCCTATCCGG GCTCCCAGGA GGAGGCGGAG 
GAGTGGGACA GACAGCGGGA CTACGCCCTG CAGGCGGCGG CAGCTCATCA CCACCATCAC 
GGACAGCCGC TGATGCAGGC GCAGCATCAT CCGCATGGAC ACGGCGGTCA TCCCCACCAG 
GTGATGCTGC AGCAGAGCAA GTATCCGCAC CACCATCACC ACCACTTCCA CAATCTGGAA 
CTGACGCCCA TCAACATGCA CTCGAACTCG TACGGTAGTG CTTCGGGTGC CCTGACGCCC 
ACCTGTCTGC CCCAAGTGCC CAGCAACGGG AGTGGCAGCA GCGGAGGCGG AGGAGGCAGT 
GGAAGCGGGA GTGGTGGACC CGGCAGCGTG GGTGGCGGCT CCTGCGTCAT CACACGGGCT 
GCCCTTCAGC CGTGCCGCCC GCTCTCCGCC AGCTCGACCA GATCCTCGAA CAACATGTCG 
CCCAGAACCT GTTCGGGTGC CTACAGTAAT GCCACGCTGG AGGATTGCCT CAATGACGAC 
ATGCTGACCA CGTTGACGGT GCGGGAGCTG AACAAGCGGC TCCATGGTTG CCCACGGGAG 
GAGGTAGTGC GTCTGAAGCA GAAGCGACGC ACCCTGAAGA ATCGCGGCTA TGCCCAGAAC 
TGCCGGTCCA AGCGACTCCA TCAGCGCCAC GAACTGGAGA AGGCCAACCG GGTGCTCAAC 
CAGGATCTGC ACCGCCTGAA GCTGGAGTAC TCGAGGGTCT GCCAGGAGCG GGATGCCCTC 
ATGCAGCGTC TGCAGCGGGC GGCAAATGGC GGAGTCGGTG CGGGAGGTGC AACCGCCGGC 
GGCGATAGCC AAAGCTCTCC GGAATTCTAC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tj ( NM_001273653.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260582.1 Drosophila melanogaster traffic jam (tj), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16887
Clone ID Related Accession (Same CDS sequence)	NM_001273653.1
Accession Version	NM_001273653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	traffic jam, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG AAGATCCCAC CATAAACGAC ACGTACGTTC AGGAGTTTGA TCTGCACCAC 
CTGGAGGTGG GCGGAAATGG AGCTGGCCAA TCGCATGGAG CCGGTGCCGC GTCCCAGGTC 
ACGACTTTGG GCCATGTGAA GCGCGAGGAT CACAGTCCAC CGCAGCCGGT GGCGGTTAAA 
TGGACGACAA TCCACGGCGA TCCCACGAAT CCGGACGATG ACCCGGACTC GCTGCCGCTG 
TCCGGACCAG AGCCCGCCGG AGCAGTGGAG GTCTCGCCAT CGCCGCACAT CAAGCTGAGG 
TCCTTCTCCG GCCATCACCA GTGGCACATG GACGAACGGC GCCTGCAACC GCTCTCTCCG 
CCACCAGAGC AGTATGGTCC GCTGCCCGGA CAGGCGATTC TGGTGAACAC ATCTTCGGGA 
GCGGGCGTTC CGGGCGGAGT GCCCTCAACG CCGCCGGAAA CGCCACCAGT AGTGGGCTCG 
CCCACGGGCA GCAGCAGCTG TCCGGCTCAG ACTTACGCAC ACCACTATGC GAGGACACCG 
GGCAGCTCCG TATCCGTATC CGCCTCCGCC GCCGCCGCAG CTGCTGCCTC CGCCGGAGCA 
GCATCGGTGA GTGCCGGACT GAGCCATGAC ATGATGTGGC TGACCAACTC GATACGGGCG 
GACCAGCAGC CGCTGGATCT GCGGCCTTTG CCCTATCCGG GCTCCCAGGA GGAGGCGGAG 
GAGTGGGACA GACAGCGGGA CTACGCCCTG CAGGCGGCGG CAGCTCATCA CCACCATCAC 
GGACAGCCGC TGATGCAGGC GCAGCATCAT CCGCATGGAC ACGGCGGTCA TCCCCACCAG 
GTGATGCTGC AGCAGAGCAA GTATCCGCAC CACCATCACC ACCACTTCCA CAATCTGGAA 
CTGACGCCCA TCAACATGCA CTCGAACTCG TACGGTAGTG CTTCGGGTGC CCTGACGCCC 
ACCTGTCTGC CCCAAGTGCC CAGCAACGGG AGTGGCAGCA GCGGAGGCGG AGGAGGCAGT 
GGAAGCGGGA GTGGTGGACC CGGCAGCGTG GGTGGCGGCT CCTGCGTCAT CACACGGGCT 
GCCCTTCAGC CGTGCCGCCC GCTCTCCGCC AGCTCGACCA GATCCTCGAA CAACATGTCG 
CCCAGAACCT GTTCGGGTGC CTACAGTAAT GCCACGCTGG AGGATTGCCT CAATGACGAC 
ATGCTGACCA CGTTGACGGT GCGGGAGCTG AACAAGCGGC TCCATGGTTG CCCACGGGAG 
GAGGTAGTGC GTCTGAAGCA GAAGCGACGC ACCCTGAAGA ATCGCGGCTA TGCCCAGAAC 
TGCCGGTCCA AGCGACTCCA TCAGCGCCAC GAACTGGAGA AGGCCAACCG GGTGCTCAAC 
CAGGATCTGC ACCGCCTGAA GCTGGAGTAC TCGAGGGTCT GCCAGGAGCG GGATGCCCTC 
ATGCAGCGTC TGCAGCGGGC GGCAAATGGC GGAGTCGGTG CGGGAGGTGC AACCGCCGGC 
GGCGATAGCC AAAGCTCTCC GGAATTCTAC CTCTGACGCC AGCTGGCGGT GGCCAGCGGC 
TCCTCCGCAT CCTCATCCTC CTCCTCGCAG CAGCAGCAGC AGCACCCCCA GCAACTTCAT 
CTCCAGCATC GGGGTCCACA GTGGAATCCC CAGCAGCTGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260582.1
CDS	490..2157
Translation	MKMEDPTINDTYVQEFDLHHLEVGGNGAGQSHGAGAASQVTTLGHVKREDHSPPQPVAVKWTTIHGDPTNPDDDPDSLPLSGPEPAGAVEVSPSPHIKLRSFSGHHQWHMDERRLQPLSPPPEQYGPLPGQAILVNTSSGAGVPGGVPSTPPETPPVVGSPTGSSSCPAQTYAHHYARTPGSSVSVSASAAAAAAASAGAASVSAGLSHDMMWLTNSIRADQQPLDLRPLPYPGSQEEAEEWDRQRDYALQAAAAHHHHHGQPLMQAQHHPHGHGGHPHQVMLQQSKYPHHHHHHFHNLELTPINMHSNSYGSASGALTPTCLPQVPSNGSGSSGGGGGSGSGSGGPGSVGGGSCVITRAALQPCRPLSASSTRSSNNMSPRTCSGAYSNATLEDCLNDDMLTTLTVRELNKRLHGCPREEVVRLKQKRRTLKNRGYAQNCRSKRLHQRHELEKANRVLNQDLHRLKLEYSRVCQERDALMQRLQRAANGGVGAGGATAGGDSQSSPEFYL*RQLAVASGSSASSSSSSQQQQQHPQQLHLQHRGPQWNPQQLVN

Target ORF information:

RefSeq Version	NM_001273653.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster traffic jam (tj), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273653.1

ORF Insert Sequence:

ATGAAAATGG AAGATCCCAC CATAAACGAC ACGTACGTTC AGGAGTTTGA TCTGCACCAC 
CTGGAGGTGG GCGGAAATGG AGCTGGCCAA TCGCATGGAG CCGGTGCCGC GTCCCAGGTC 
ACGACTTTGG GCCATGTGAA GCGCGAGGAT CACAGTCCAC CGCAGCCGGT GGCGGTTAAA 
TGGACGACAA TCCACGGCGA TCCCACGAAT CCGGACGATG ACCCGGACTC GCTGCCGCTG 
TCCGGACCAG AGCCCGCCGG AGCAGTGGAG GTCTCGCCAT CGCCGCACAT CAAGCTGAGG 
TCCTTCTCCG GCCATCACCA GTGGCACATG GACGAACGGC GCCTGCAACC GCTCTCTCCG 
CCACCAGAGC AGTATGGTCC GCTGCCCGGA CAGGCGATTC TGGTGAACAC ATCTTCGGGA 
GCGGGCGTTC CGGGCGGAGT GCCCTCAACG CCGCCGGAAA CGCCACCAGT AGTGGGCTCG 
CCCACGGGCA GCAGCAGCTG TCCGGCTCAG ACTTACGCAC ACCACTATGC GAGGACACCG 
GGCAGCTCCG TATCCGTATC CGCCTCCGCC GCCGCCGCAG CTGCTGCCTC CGCCGGAGCA 
GCATCGGTGA GTGCCGGACT GAGCCATGAC ATGATGTGGC TGACCAACTC GATACGGGCG 
GACCAGCAGC CGCTGGATCT GCGGCCTTTG CCCTATCCGG GCTCCCAGGA GGAGGCGGAG 
GAGTGGGACA GACAGCGGGA CTACGCCCTG CAGGCGGCGG CAGCTCATCA CCACCATCAC 
GGACAGCCGC TGATGCAGGC GCAGCATCAT CCGCATGGAC ACGGCGGTCA TCCCCACCAG 
GTGATGCTGC AGCAGAGCAA GTATCCGCAC CACCATCACC ACCACTTCCA CAATCTGGAA 
CTGACGCCCA TCAACATGCA CTCGAACTCG TACGGTAGTG CTTCGGGTGC CCTGACGCCC 
ACCTGTCTGC CCCAAGTGCC CAGCAACGGG AGTGGCAGCA GCGGAGGCGG AGGAGGCAGT 
GGAAGCGGGA GTGGTGGACC CGGCAGCGTG GGTGGCGGCT CCTGCGTCAT CACACGGGCT 
GCCCTTCAGC CGTGCCGCCC GCTCTCCGCC AGCTCGACCA GATCCTCGAA CAACATGTCG 
CCCAGAACCT GTTCGGGTGC CTACAGTAAT GCCACGCTGG AGGATTGCCT CAATGACGAC 
ATGCTGACCA CGTTGACGGT GCGGGAGCTG AACAAGCGGC TCCATGGTTG CCCACGGGAG 
GAGGTAGTGC GTCTGAAGCA GAAGCGACGC ACCCTGAAGA ATCGCGGCTA TGCCCAGAAC 
TGCCGGTCCA AGCGACTCCA TCAGCGCCAC GAACTGGAGA AGGCCAACCG GGTGCTCAAC 
CAGGATCTGC ACCGCCTGAA GCTGGAGTAC TCGAGGGTCT GCCAGGAGCG GGATGCCCTC 
ATGCAGCGTC TGCAGCGGGC GGCAAATGGC GGAGTCGGTG CGGGAGGTGC AACCGCCGGC 
GGCGATAGCC AAAGCTCTCC GGAATTCTAC CTCTGACGCC AGCTGGCGGT GGCCAGCGGC 
TCCTCCGCAT CCTCATCCTC CTCCTCGCAG CAGCAGCAGC AGCACCCCCA GCAACTTCAT 
CTCCAGCATC GGGGTCCACA GTGGAATCCC CAGCAGCTGG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tj cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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