brat cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	brat
Entrez Gene ID	35197
Full Name	brain tumor
Synonyms	BRAT,Brat,CG10719,Cf,Dmel\CG10719,E60,anon-37CDa,fs(2)ltoPM43,l(2)37Cf,l(2)E60,l(2)brat,lincRNA.S1565
General protein information	Preferred Names brain tumor Names CG10719 gene product from transcript CG10719-RE CG10719-PA CG10719-PB CG10719-PC CG10719-PE CG10719-PF brain tumour brat-PA brat-PB brat-PC brat-PE brat-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37C1-37C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_057597.4	NP_476945.1	brain tumor, isoform A
NM_001201913.2	NP_001188842.2	brain tumor, isoform E
NM_134302.2	NP_599129.1	brain tumor, isoform B
NM_206004.2	NP_995726.1	brain tumor, isoform C
NM_001299154.1	NP_001286083.1	brain tumor, isoform F

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010054	XP_319208.4
Drosophila melanogaster (fruit fly)	brat	NP_995726.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results support a novel role for Brat/TRIM3 in maintaining stem cell equilibrium and suppressing tumor growth by regulating NICD nuclear transport
Title: Drosophila Brat and Human Ortholog TRIM3 Maintain Stem Cell Equilibrium and Suppress Brain Tumorigenesis by Attenuating Notch Nuclear Transport.

BRAT is a prominent post-transcriptional regulator in the early embryo through mechanisms that are largely independent of PUM.
Title: Brain tumor is a sequence-specific RNA-binding protein that directs maternal mRNA clearance during the Drosophila maternal-to-zygotic transition.

that Brat represses the translation of src64B, an upstream regulator of a conserved Rho-dependent pathway previously shown to promote axon retraction
Title: The TRIM-NHL protein Brat promotes axon maintenance by repressing src64B expression.

the Brat-dependent mechanism extinguishes the function of the self-renewal factor Klu in the presumptive intermediate progenitor cell by attenuating Arm activity, balancing stem cell maintenance and progenitor cell specification.
Title: Brain tumor specifies intermediate progenitor cell identity by attenuating ?-catenin/Armadillo activity.

These results together reveal an important and previously unidentified role for Brat in synaptic development and endocytosis mediated by suppression of BMP signaling.
Title: Brain tumor regulates neuromuscular synapse growth and endocytosis in Drosophila by suppressing mad expression.

The following brat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14942	NM_001201913.2 Latest version!	Drosophila melanogaster brain tumor (brat), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa16056	NM_001299154.1 Latest version!	Drosophila melanogaster brain tumor (brat), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa16056	NM_134302.2 Latest version!	Drosophila melanogaster brain tumor (brat), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa16056	NM_206004.2 Latest version!	Drosophila melanogaster brain tumor (brat), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa16056	NM_057597.4 Latest version!	Drosophila melanogaster brain tumor (brat), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brat ( NM_001201913.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188842.2 Drosophila melanogaster brain tumor (brat), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa14942
Clone ID Related Accession (Same CDS sequence)	NM_001201913.2
Accession Version	NM_001201913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brain tumor, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201913.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGTATT GCACAAATTG 
CACAGCAACA TCAATGAAAC CAGCAAGAGC TTCTTGGCAG ACAGAATACA GCAACATTTC 
ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188842.2
CDS	838..4023
Translation	MASSPTPSLDSMRGGANSIESYEHAGYLSDSPLTLSGSSPPASDSAICSDEYTGGSSVKSRSEVTVINGHHPISASVSSSSSASSSSCSSSSSSSSSSSSSSSSTSGLSGCGSTSSSVISANNVASSNGPGVIGSNLQSSNNGGNSGISSLVVGAGKGSNSSSNSSSSNTSANGSPPRCTACKSKCSDAVAKCFECQSYLCANCVTAHEFMHCFNGHNVCLIKGFEASTTGTPLSVGSPSNNPASNEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQREDSIYGSLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTTSTGSTANESALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELNETHQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSLLTEALLLRKSLEQQLQTGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSNGHGKQPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLSGASLLDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHYNPYEKWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVVSGAGAGGSTVSSESLLDLTNKLLSATIYPPKSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEGRFKFQFGECGKRDSQLLYPNRVAVVRNSGDIIVTERSPTHQIQIYNQYGQFVRKFGATILQHPRGVTVDNKGRIIVVECKVMRVIIFDQNGNVLHKFGCSKHLEFPNGVVVNDKQEIFISDNRAHCVKVFNYEGQYLRQIGGEGITNYPIGVGINSNGEILIADNHNNFNLTIFTQDGQLISALESKVKHAQCFDVALMDDGSVVLASKDYRLYIYRYVQLAPVVLHKLHSNINETSKSFLADRIQQHFI

Target ORF information:

RefSeq Version	NM_001201913.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brain tumor (brat), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201913.2

ORF Insert Sequence:

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGTATT GCACAAATTG 
CACAGCAACA TCAATGAAAC CAGCAAGAGC TTCTTGGCAG ACAGAATACA GCAACATTTC 
ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brat ( NM_057597.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476945.1 Drosophila melanogaster brain tumor (brat), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16056
Clone ID Related Accession (Same CDS sequence)	NM_057597.4 , NM_134302.2 , NM_206004.2 , NM_001299154.1
Accession Version	NM_057597.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brain tumor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057597.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGGTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476945.1
CDS	838..3951
Translation	MASSPTPSLDSMRGGANSIESYEHAGYLSDSPLTLSGSSPPASDSAICSDEYTGGSSVKSRSEVTVINGHHPISASVSSSSSASSSSCSSSSSSSSSSSSSSSSTSGLSGCGSTSSSVISANNVASSNGPGVIGSNLQSSNNGGNSGISSLVVGAGKGSNSSSNSSSSNTSANGSPPRCTACKSKCSDAVAKCFECQSYLCANCVTAHEFMHCFNGHNVCLIKGFEASTTGTPLSVGSPSNNPASNEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQREDSIYGSLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTTSTGSTANESALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELNETHQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSLLTEALLLRKSLEQQLQTGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSNGHGKQPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLSGASLLDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHYNPYEKWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVVSGAGAGGSTVSSESLLDLTNKLLSATIYPPKSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEGRFKFQFGECGKRDSQLLYPNRVAVVRNSGDIIVTERSPTHQIQIYNQYGQFVRKFGATILQHPRGVTVDNKGRIIVVECKVMRVIIFDQNGNVLHKFGCSKHLEFPNGVVVNDKQEIFISDNRAHCVKVFNYEGQYLRQIGGEGITNYPIGVGINSNGEILIADNHNNFNLTIFTQDGQLISALESKVKHAQCFDVALMDDGSVVLASKDYRLYIYRYVQLAPVGM

Target ORF information:

RefSeq Version	NM_057597.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brain tumor (brat), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057597.4

ORF Insert Sequence:

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGGTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brat ( NM_134302.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599129.1 Drosophila melanogaster brain tumor (brat), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16056
Clone ID Related Accession (Same CDS sequence)	NM_057597.4 , NM_134302.2 , NM_206004.2 , NM_001299154.1
Accession Version	NM_134302.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brain tumor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134302.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGGTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599129.1
CDS	542..3655
Translation	MASSPTPSLDSMRGGANSIESYEHAGYLSDSPLTLSGSSPPASDSAICSDEYTGGSSVKSRSEVTVINGHHPISASVSSSSSASSSSCSSSSSSSSSSSSSSSSTSGLSGCGSTSSSVISANNVASSNGPGVIGSNLQSSNNGGNSGISSLVVGAGKGSNSSSNSSSSNTSANGSPPRCTACKSKCSDAVAKCFECQSYLCANCVTAHEFMHCFNGHNVCLIKGFEASTTGTPLSVGSPSNNPASNEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQREDSIYGSLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTTSTGSTANESALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELNETHQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSLLTEALLLRKSLEQQLQTGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSNGHGKQPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLSGASLLDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHYNPYEKWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVVSGAGAGGSTVSSESLLDLTNKLLSATIYPPKSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEGRFKFQFGECGKRDSQLLYPNRVAVVRNSGDIIVTERSPTHQIQIYNQYGQFVRKFGATILQHPRGVTVDNKGRIIVVECKVMRVIIFDQNGNVLHKFGCSKHLEFPNGVVVNDKQEIFISDNRAHCVKVFNYEGQYLRQIGGEGITNYPIGVGINSNGEILIADNHNNFNLTIFTQDGQLISALESKVKHAQCFDVALMDDGSVVLASKDYRLYIYRYVQLAPVGM

Target ORF information:

RefSeq Version	NM_134302.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brain tumor (brat), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134302.2

ORF Insert Sequence:

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGGTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brat ( NM_206004.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995726.1 Drosophila melanogaster brain tumor (brat), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16056
Clone ID Related Accession (Same CDS sequence)	NM_057597.4 , NM_134302.2 , NM_206004.2 , NM_001299154.1
Accession Version	NM_206004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brain tumor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_206004.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGGTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995726.1
CDS	476..3589
Translation	MASSPTPSLDSMRGGANSIESYEHAGYLSDSPLTLSGSSPPASDSAICSDEYTGGSSVKSRSEVTVINGHHPISASVSSSSSASSSSCSSSSSSSSSSSSSSSSTSGLSGCGSTSSSVISANNVASSNGPGVIGSNLQSSNNGGNSGISSLVVGAGKGSNSSSNSSSSNTSANGSPPRCTACKSKCSDAVAKCFECQSYLCANCVTAHEFMHCFNGHNVCLIKGFEASTTGTPLSVGSPSNNPASNEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQREDSIYGSLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTTSTGSTANESALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELNETHQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSLLTEALLLRKSLEQQLQTGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSNGHGKQPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLSGASLLDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHYNPYEKWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVVSGAGAGGSTVSSESLLDLTNKLLSATIYPPKSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEGRFKFQFGECGKRDSQLLYPNRVAVVRNSGDIIVTERSPTHQIQIYNQYGQFVRKFGATILQHPRGVTVDNKGRIIVVECKVMRVIIFDQNGNVLHKFGCSKHLEFPNGVVVNDKQEIFISDNRAHCVKVFNYEGQYLRQIGGEGITNYPIGVGINSNGEILIADNHNNFNLTIFTQDGQLISALESKVKHAQCFDVALMDDGSVVLASKDYRLYIYRYVQLAPVGM

Target ORF information:

RefSeq Version	NM_206004.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brain tumor (brat), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206004.2

ORF Insert Sequence:

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGGTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brat ( NM_001299154.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286083.1 Drosophila melanogaster brain tumor (brat), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa16056
Clone ID Related Accession (Same CDS sequence)	NM_057597.4 , NM_134302.2 , NM_206004.2 , NM_001299154.1
Accession Version	NM_001299154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brain tumor, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGGTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286083.1
CDS	448..3561
Translation	MASSPTPSLDSMRGGANSIESYEHAGYLSDSPLTLSGSSPPASDSAICSDEYTGGSSVKSRSEVTVINGHHPISASVSSSSSASSSSCSSSSSSSSSSSSSSSSTSGLSGCGSTSSSVISANNVASSNGPGVIGSNLQSSNNGGNSGISSLVVGAGKGSNSSSNSSSSNTSANGSPPRCTACKSKCSDAVAKCFECQSYLCANCVTAHEFMHCFNGHNVCLIKGFEASTTGTPLSVGSPSNNPASNEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQREDSIYGSLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTTSTGSTANESALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELNETHQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSLLTEALLLRKSLEQQLQTGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSNGHGKQPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLSGASLLDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHYNPYEKWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVVSGAGAGGSTVSSESLLDLTNKLLSATIYPPKSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEGRFKFQFGECGKRDSQLLYPNRVAVVRNSGDIIVTERSPTHQIQIYNQYGQFVRKFGATILQHPRGVTVDNKGRIIVVECKVMRVIIFDQNGNVLHKFGCSKHLEFPNGVVVNDKQEIFISDNRAHCVKVFNYEGQYLRQIGGEGITNYPIGVGINSNGEILIADNHNNFNLTIFTQDGQLISALESKVKHAQCFDVALMDDGSVVLASKDYRLYIYRYVQLAPVGM

Target ORF information:

RefSeq Version	NM_001299154.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brain tumor (brat), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299154.1

ORF Insert Sequence:

ATGGCGTCCT CACCGACACC ATCTCTGGAC TCGATGCGGG GCGGGGCGAA CTCGATTGAA 
TCGTACGAAC ACGCCGGCTA TCTATCAGAC TCTCCGCTCA CGTTGAGCGG TTCATCGCCA 
CCGGCAAGCG ATTCGGCAAT CTGCAGTGAT GAGTACACGG GCGGCAGCAG CGTTAAATCA 
CGCTCTGAGG TGACTGTAAT CAATGGCCAT CATCCCATTT CGGCCTCCGT ATCGTCCAGC 
TCTTCGGCCA GCTCTTCATC CTGCTCGTCG TCATCGTCCT CATCCTCGTC TTCGTCATCG 
TCCAGCTCCT CGACCAGCGG CCTCAGCGGT TGCGGCAGTA CCAGCAGCAG TGTGATCAGC 
GCCAACAACG TTGCGAGCAG CAATGGACCA GGCGTTATCG GCAGCAATCT TCAGTCCTCA 
AACAATGGCG GCAACTCGGG CATCAGCAGT CTGGTCGTGG GTGCGGGCAA GGGAAGCAAC 
AGCAGCAGCA ACAGCTCCTC GAGCAACACA TCCGCCAATG GATCTCCGCC ACGTTGCACC 
GCCTGCAAGA GCAAGTGCAG CGATGCGGTG GCCAAGTGCT TCGAATGCCA GAGCTACCTG 
TGCGCCAATT GTGTAACTGC TCATGAGTTC ATGCACTGCT TCAACGGACA CAATGTGTGC 
CTGATTAAGG GTTTTGAGGC GAGCACCACT GGCACTCCGC TGTCGGTTGG ATCGCCTAGC 
AACAATCCGG CGTCGAATGA GTTCAAGTAC GCCAGTTCGC TGACCATGAT GCTGCAGCAG 
CAGCAACAGC TCGACTCGCA ACAACAGCAA CAGCAGCAAC AATTGCTGCC GGCGCAGCCT 
ATGTCGCAAC TCTCGAAGAT TGTACTGGCA GCCGCCGCGC AGGCAAATTC CCAGGAGCAA 
CAGCGAGAGG ATAGCATCTA TGGTTCACTG CACCCACAAC AACAACAACA GCAGCAGCAA 
CAGCAGCAGC GACAACTCTT CTGCCCCCGA CACAAACAGG AGCTGCTCAA GTTCAGTTGC 
CGCACCTGCT GCATCCTGGT GTGCAAGGAG TGCATTGTAC TGGAGCACTC CACAGGTCTC 
CACGAACTGG AGAACGTGCA GTCCCCCGGA ATGACAACCT CTACGGGATC CACAGCCAAC 
GAGTCAGCTT TGCAGACTCT GCTCGCCGAT ATGCGTGGCA AAATTGGCGA GATTGTCGGC 
ATTGCTGGAA ACTCGGACCA GAATCTCACC AAGGTGAAGC TGCAGTACCA AAAGGCTCAC 
AACGAGCTGA ACGAGACGCA CCAGTTCTTC GCTTCCATGC TGGACGAGCG CAAGACCGAA 
TTGCTCAAGG AACTGGAGAC GCTGTACACG GCCAAGGTTA ACAGCAACAA TTCGTGGCAG 
CAACGCTCCC GCGACTTGAT CGACAAGGGA CTGGCCACCT GCGAGGCGGT CGAGCGGAGT 
CCTGCTCCGC CATCATCCTT GCTGACAGAG GCCCTTCTCC TGCGCAAATC CCTGGAGCAA 
CAACTACAGA CGGGCATTCA GGAGATGCAG CTACCATTCG AGATCGAATT TATGTCCAAC 
TATCAGTCGA TTCAGGCCGG CGTGCGCAAC ACCTTTGGTT ACATTCGGGC AAACAGCTCA 
GATGGTGGAC CTACTGGCAT GAGCCTGACG AGCAATGGCC ACGGAAAGCA GCCGCCAATT 
GCCCGACCAA CACAGAGCGC CAGCAACAGC AGTGCGAGCA GCGCGGGCAG TGGTCACCAT 
GGCCACCACC AACAGTCACA CCATCATGGC CACCACAATC ATCACCAGAC GGCCCATCAT 
CAACAGCTGC AGGCGCAATC GTCGCTGCAT GGCTTGGGTC TTGGATTGAG TGGCGCCAGT 
TTGTTGGATA GCAGCTCTAG CGCAGGCGGA GCAGTGGGAG CCTTCAGCAA CGGAGGACTT 
CTGCTGGGCG GTCGCGATCG GAACGCTCTG GCAGTGGAAC AGCATTTCGG TGAGCTGATG 
CCCAAGCGAG GAGGAGGTGG CTACACGGGC AGCAATGGAT CGGCCACCAG TGCCGTGGCC 
CACTACAATC CCTACGAGAA GTGGAGCAAC GGCGGTAGCG ACAATCTGTT TTCATCGGTG 
ACAAGCGGCG TGTCCGGTAG CTCTGCAGTG GCCGATGCAT TTGCCAGCCT GTCTGCTGTT 
GGAGGATCCG TGGTTAGTGG CGCTGGAGCT GGCGGAAGTA CTGTGAGCTC AGAGTCGTTG 
CTGGACCTCA CCAACAAGCT GCTTTCGGCC ACTATCTATC CGCCCAAGTC GCAGATCAAG 
CGACAGAAGA TGATTTACCA CTGCAAGTTT GGCGAATTCG GCGTGATGGA GGGCCAGTTC 
ACGGAGCCCA GCGGTGTGGC GGTAAATGCC CAGAATGACA TCATTGTTGC GGATACGAAC 
AACCATCGCA TTCAGATCTT CGACAAGGAG GGACGCTTCA AGTTCCAGTT TGGCGAGTGC 
GGCAAGCGTG ACTCGCAGCT GCTCTATCCG AACCGCGTGG CCGTGGTGCG CAATTCCGGC 
GATATTATTG TCACCGAGCG CTCGCCCACA CACCAGATAC AGATCTACAA TCAGTACGGC 
CAGTTTGTGA GGAAATTCGG GGCCACCATT CTGCAGCATC CTCGCGGCGT GACCGTGGAC 
AACAAGGGAC GGATCATTGT GGTGGAATGC AAGGTGATGC GTGTGATCAT CTTTGATCAG 
AACGGCAATG TGCTGCACAA GTTCGGGTGC TCTAAGCACC TCGAGTTCCC CAACGGCGTG 
GTGGTCAACG ACAAGCAGGA GATCTTCATC AGCGACAATC GGGCGCACTG TGTCAAGGTG 
TTCAACTACG AGGGCCAGTA TCTGCGGCAG ATTGGCGGCG AGGGTATTAC CAACTACCCA 
ATTGGCGTCG GGATCAACTC GAACGGGGAG ATCCTCATCG CGGACAACCA CAACAACTTC 
AACCTGACGA TCTTCACGCA GGACGGGCAG CTGATCTCGG CGCTCGAGTC GAAGGTGAAG 
CACGCGCAGT GCTTCGATGT GGCGCTCATG GACGACGGCA GTGTGGTGCT GGCCAGCAAG 
GACTATCGGC TCTACATATA TCGCTATGTC CAACTGGCGC CAGTGGGTAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

brat cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報