CG10431 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG10431
Entrez Gene ID	35157
Full Name	CG10431 gene product from transcript CG10431-RA
Synonyms	Dmel\CG10431,dmCG10431
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37B8-37B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299149.1	NP_001286078.1	uncharacterized protein, isoform B
NM_136080.3	NP_609924.2	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG10431 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG10431 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16027	NM_136080.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG10431), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16027	NM_001299149.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG10431), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG10431 ( NM_001299149.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286078.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG10431), mRNA.

Price & Availability↑

CloneID	OFa16027
Clone ID Related Accession (Same CDS sequence)	NM_001299149.1 , NM_136080.3
Accession Version	NM_001299149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGCCC ACTGCGCCGT GATAAATTGC AGCCATAAGT ATGTGCACGC GGGCAGCATT 
TCATTTCACA GATTTCCATT CAAACGGAAG GATCTTCTAC AGAAATGGAA GGAATTTACC 
CAGAGGAGTG CTCAGTGGAT GCCCTCAAAA TGGAGTGCGC TGTGCAGTCG GCACTTTGGT 
GATGAGGACT TTAATTGCAG CAATAATCGG AAAACGCTTA AGAAAAATGC TGTGCCATCA 
ATTAGGGTTT CGGAAGATGA CTCCATGAGT GGGCACTTGG AACAGGTGTC CCCAAGCAAT 
AGGCCAACGC ACAAGCAAAC TCTTTCTAGT GCCGCCGAAT CAGCAGCAAC AGGTGTTAAA 
GCCACTTGCC GCTTTTGTGG TTCCTCGGCC ATCAATTGCT CCTCGTTTGA CAAGAGTTTT 
GAGCTCTTTG GCATGATCCA GAAGTGCTTT CCTACGCTAC AGATTCTTCA GGACGACACC 
TTGCCCAAGG AAATATGCCA GGAGTGCTGC CTCCAATTGG AGCGCTTTTC CCAGTTCATC 
GATGTGGTGA TGTTGGCGCA GAGTGAATTG CAAAGAAAAT ACCGCCGACA ACTGAAAATC 
AAACAGGAAC CCTTGGTTCG TGTGAAACAG GAGGCCTGCG AGAGCTTGGA CAATCTTTTT 
CCTGATGAAC TGGACATGGG TTTGGAGCAG GACGATGGCT GCGATCAGGA AGAAACTGAA 
CAAAAGTTTC AGTTCTGCGA GTTCCCTATG CTAAATGCCC AAGACATTAT CAACAATTGT 
GATATCATGG AGATAATCAA TCTGGACGAT CCGTTTATCA ATATTCCAGA CGATGCGGAT 
GTGGGACAGT CGGAGAGACT ACAGCCTGGA AGAACGGCTA ATGAAATGCT GCAAAACGAA 
CTCCTAACCG AAGAGCACAA TTATGCGAAG GAGGAGTGGC AACTACCCCC GCATCAGTAC 
AAAACGGAAA AGGTGGAGGG AACGGATGCT GCGCCAGACG CAGCTCCTCC GCCTGCTGAT 
CCCCCTCCTC TTCAGGATAA TATAGACCCT CCCTCGGCCC CCGAACCGCG CTCATGCAAG 
CCCATTGTGA CCAATGTAAG CCAGATACCT AACCCAATGA TCTGCGAGCT GCTGCCACAG 
CCTGCTCCTC CCACGTCGAT CAAACCCAAC ATCGTGGTAC TTAACGATAG TGTGGTCGAA 
TCCTCAGCGG CTTTTCGGCT TCACAACTGC ACTTTGTGCA CTGCCAAATT TATAACAATA 
GATAGCTTAA ATCAGCATTA TGCACACAGT CACAGTAATT CCACTCCCAT GGTTAGTGTT 
CCTCCGATGA ATATATGCCT TCCCAGCTTA ACCATGAACC CTCCAATGAA AATGGAAAGC 
AACAGAGGTC TTGTGGCCAC TGAACAATCC GAATACTGGC CGTTGGAGTG GCATAATAGT 
CCTGTATCCA AGCAACCTAG GAAAAAGATT GATATACTAG ATATGCAGAG TAGTTTCCTG 
CATCACAAAG ACCTTATACC GACAGCCACT CAACTGTCAG CACCCGCACC TGATCCTCAA 
CTGGTTGCAG TGACTGCTAG CTACGCTAAA CTGGCTAACA GGTATCGTAA GTTGCAGCTG 
AAATGCAAAA AACTGAAGTC ACCAAGGAAG GTGCACAGAA AAACCTATGT GTGCCGGATG 
TGCAGGAAGG GATTTTCAAA GTTTAAGAAC TTACACCACC ACCGTCGTCA GAAAGCACAC 
TTTGTAAAGT TAATACCCAA TTTTTCAGGA CGCTGTTCTG GTTGCCTAAA GTTTTTTCGA 
TCCCGCTTGG GTCTTCGCCA GCATATGCGA TACATATGTC AATCGTTGTC GCTCAAAAAC 
CATCGGCGCC TTCAGAGCTT CAAGTGTCGC CACTGCCAGG CCATCGCCTT TGCCCACTGG 
CGCCTGTATC GTCGTCATGA ACTCAATTGC AGGCCCAAAA AATCCAAAAC TAAGGTGCAG 
ACGGCAATGA ATTCGAAAAA GAAAGTTACG CCCACCCAAG TATTTGAGTG TAATATTTGT 
AAAAAATCAT TCGGTTCGCT CAACGGACTA CGCCAGCATA ACATCACCCA CTCGACGGAG 
CGGCAACACA AGTGCGGCAT CTGTGAGCGG GTCTTCAAGC GACGCAACGG ATTGTCTCAA 
CACATTAAGG GCTACCACCT GCAGCTCAAG CCGCATGAGT GTCCGGTTTG CCAGCATCGC 
TACGCCCTGA AATGCGATAT GCTGAGGTGC AGGCATTCCC TGCGAAAAGG TCCAGCTGCC 
GGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286078.1
CDS	68..2356
Translation	MPAHCAVINCSHKYVHAGSISFHRFPFKRKDLLQKWKEFTQRSAQWMPSKWSALCSRHFGDEDFNCSNNRKTLKKNAVPSIRVSEDDSMSGHLEQVSPSNRPTHKQTLSSAAESAATGVKATCRFCGSSAINCSSFDKSFELFGMIQKCFPTLQILQDDTLPKEICQECCLQLERFSQFIDVVMLAQSELQRKYRRQLKIKQEPLVRVKQEACESLDNLFPDELDMGLEQDDGCDQEETEQKFQFCEFPMLNAQDIINNCDIMEIINLDDPFINIPDDADVGQSERLQPGRTANEMLQNELLTEEHNYAKEEWQLPPHQYKTEKVEGTDAAPDAAPPPADPPPLQDNIDPPSAPEPRSCKPIVTNVSQIPNPMICELLPQPAPPTSIKPNIVVLNDSVVESSAAFRLHNCTLCTAKFITIDSLNQHYAHSHSNSTPMVSVPPMNICLPSLTMNPPMKMESNRGLVATEQSEYWPLEWHNSPVSKQPRKKIDILDMQSSFLHHKDLIPTATQLSAPAPDPQLVAVTASYAKLANRYRKLQLKCKKLKSPRKVHRKTYVCRMCRKGFSKFKNLHHHRRQKAHFVKLIPNFSGRCSGCLKFFRSRLGLRQHMRYICQSLSLKNHRRLQSFKCRHCQAIAFAHWRLYRRHELNCRPKKSKTKVQTAMNSKKKVTPTQVFECNICKKSFGSLNGLRQHNITHSTERQHKCGICERVFKRRNGLSQHIKGYHLQLKPHECPVCQHRYALKCDMLRCRHSLRKGPAAGE

Target ORF information:

RefSeq Version	NM_001299149.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG10431), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299149.1

ORF Insert Sequence:

ATGCCCGCCC ACTGCGCCGT GATAAATTGC AGCCATAAGT ATGTGCACGC GGGCAGCATT 
TCATTTCACA GATTTCCATT CAAACGGAAG GATCTTCTAC AGAAATGGAA GGAATTTACC 
CAGAGGAGTG CTCAGTGGAT GCCCTCAAAA TGGAGTGCGC TGTGCAGTCG GCACTTTGGT 
GATGAGGACT TTAATTGCAG CAATAATCGG AAAACGCTTA AGAAAAATGC TGTGCCATCA 
ATTAGGGTTT CGGAAGATGA CTCCATGAGT GGGCACTTGG AACAGGTGTC CCCAAGCAAT 
AGGCCAACGC ACAAGCAAAC TCTTTCTAGT GCCGCCGAAT CAGCAGCAAC AGGTGTTAAA 
GCCACTTGCC GCTTTTGTGG TTCCTCGGCC ATCAATTGCT CCTCGTTTGA CAAGAGTTTT 
GAGCTCTTTG GCATGATCCA GAAGTGCTTT CCTACGCTAC AGATTCTTCA GGACGACACC 
TTGCCCAAGG AAATATGCCA GGAGTGCTGC CTCCAATTGG AGCGCTTTTC CCAGTTCATC 
GATGTGGTGA TGTTGGCGCA GAGTGAATTG CAAAGAAAAT ACCGCCGACA ACTGAAAATC 
AAACAGGAAC CCTTGGTTCG TGTGAAACAG GAGGCCTGCG AGAGCTTGGA CAATCTTTTT 
CCTGATGAAC TGGACATGGG TTTGGAGCAG GACGATGGCT GCGATCAGGA AGAAACTGAA 
CAAAAGTTTC AGTTCTGCGA GTTCCCTATG CTAAATGCCC AAGACATTAT CAACAATTGT 
GATATCATGG AGATAATCAA TCTGGACGAT CCGTTTATCA ATATTCCAGA CGATGCGGAT 
GTGGGACAGT CGGAGAGACT ACAGCCTGGA AGAACGGCTA ATGAAATGCT GCAAAACGAA 
CTCCTAACCG AAGAGCACAA TTATGCGAAG GAGGAGTGGC AACTACCCCC GCATCAGTAC 
AAAACGGAAA AGGTGGAGGG AACGGATGCT GCGCCAGACG CAGCTCCTCC GCCTGCTGAT 
CCCCCTCCTC TTCAGGATAA TATAGACCCT CCCTCGGCCC CCGAACCGCG CTCATGCAAG 
CCCATTGTGA CCAATGTAAG CCAGATACCT AACCCAATGA TCTGCGAGCT GCTGCCACAG 
CCTGCTCCTC CCACGTCGAT CAAACCCAAC ATCGTGGTAC TTAACGATAG TGTGGTCGAA 
TCCTCAGCGG CTTTTCGGCT TCACAACTGC ACTTTGTGCA CTGCCAAATT TATAACAATA 
GATAGCTTAA ATCAGCATTA TGCACACAGT CACAGTAATT CCACTCCCAT GGTTAGTGTT 
CCTCCGATGA ATATATGCCT TCCCAGCTTA ACCATGAACC CTCCAATGAA AATGGAAAGC 
AACAGAGGTC TTGTGGCCAC TGAACAATCC GAATACTGGC CGTTGGAGTG GCATAATAGT 
CCTGTATCCA AGCAACCTAG GAAAAAGATT GATATACTAG ATATGCAGAG TAGTTTCCTG 
CATCACAAAG ACCTTATACC GACAGCCACT CAACTGTCAG CACCCGCACC TGATCCTCAA 
CTGGTTGCAG TGACTGCTAG CTACGCTAAA CTGGCTAACA GGTATCGTAA GTTGCAGCTG 
AAATGCAAAA AACTGAAGTC ACCAAGGAAG GTGCACAGAA AAACCTATGT GTGCCGGATG 
TGCAGGAAGG GATTTTCAAA GTTTAAGAAC TTACACCACC ACCGTCGTCA GAAAGCACAC 
TTTGTAAAGT TAATACCCAA TTTTTCAGGA CGCTGTTCTG GTTGCCTAAA GTTTTTTCGA 
TCCCGCTTGG GTCTTCGCCA GCATATGCGA TACATATGTC AATCGTTGTC GCTCAAAAAC 
CATCGGCGCC TTCAGAGCTT CAAGTGTCGC CACTGCCAGG CCATCGCCTT TGCCCACTGG 
CGCCTGTATC GTCGTCATGA ACTCAATTGC AGGCCCAAAA AATCCAAAAC TAAGGTGCAG 
ACGGCAATGA ATTCGAAAAA GAAAGTTACG CCCACCCAAG TATTTGAGTG TAATATTTGT 
AAAAAATCAT TCGGTTCGCT CAACGGACTA CGCCAGCATA ACATCACCCA CTCGACGGAG 
CGGCAACACA AGTGCGGCAT CTGTGAGCGG GTCTTCAAGC GACGCAACGG ATTGTCTCAA 
CACATTAAGG GCTACCACCT GCAGCTCAAG CCGCATGAGT GTCCGGTTTG CCAGCATCGC 
TACGCCCTGA AATGCGATAT GCTGAGGTGC AGGCATTCCC TGCGAAAAGG TCCAGCTGCC 
GGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG10431 ( NM_136080.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609924.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG10431), mRNA.

Price & Availability↑

CloneID	OFa16027
Clone ID Related Accession (Same CDS sequence)	NM_001299149.1 , NM_136080.3
Accession Version	NM_136080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_136080.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGCCC ACTGCGCCGT GATAAATTGC AGCCATAAGT ATGTGCACGC GGGCAGCATT 
TCATTTCACA GATTTCCATT CAAACGGAAG GATCTTCTAC AGAAATGGAA GGAATTTACC 
CAGAGGAGTG CTCAGTGGAT GCCCTCAAAA TGGAGTGCGC TGTGCAGTCG GCACTTTGGT 
GATGAGGACT TTAATTGCAG CAATAATCGG AAAACGCTTA AGAAAAATGC TGTGCCATCA 
ATTAGGGTTT CGGAAGATGA CTCCATGAGT GGGCACTTGG AACAGGTGTC CCCAAGCAAT 
AGGCCAACGC ACAAGCAAAC TCTTTCTAGT GCCGCCGAAT CAGCAGCAAC AGGTGTTAAA 
GCCACTTGCC GCTTTTGTGG TTCCTCGGCC ATCAATTGCT CCTCGTTTGA CAAGAGTTTT 
GAGCTCTTTG GCATGATCCA GAAGTGCTTT CCTACGCTAC AGATTCTTCA GGACGACACC 
TTGCCCAAGG AAATATGCCA GGAGTGCTGC CTCCAATTGG AGCGCTTTTC CCAGTTCATC 
GATGTGGTGA TGTTGGCGCA GAGTGAATTG CAAAGAAAAT ACCGCCGACA ACTGAAAATC 
AAACAGGAAC CCTTGGTTCG TGTGAAACAG GAGGCCTGCG AGAGCTTGGA CAATCTTTTT 
CCTGATGAAC TGGACATGGG TTTGGAGCAG GACGATGGCT GCGATCAGGA AGAAACTGAA 
CAAAAGTTTC AGTTCTGCGA GTTCCCTATG CTAAATGCCC AAGACATTAT CAACAATTGT 
GATATCATGG AGATAATCAA TCTGGACGAT CCGTTTATCA ATATTCCAGA CGATGCGGAT 
GTGGGACAGT CGGAGAGACT ACAGCCTGGA AGAACGGCTA ATGAAATGCT GCAAAACGAA 
CTCCTAACCG AAGAGCACAA TTATGCGAAG GAGGAGTGGC AACTACCCCC GCATCAGTAC 
AAAACGGAAA AGGTGGAGGG AACGGATGCT GCGCCAGACG CAGCTCCTCC GCCTGCTGAT 
CCCCCTCCTC TTCAGGATAA TATAGACCCT CCCTCGGCCC CCGAACCGCG CTCATGCAAG 
CCCATTGTGA CCAATGTAAG CCAGATACCT AACCCAATGA TCTGCGAGCT GCTGCCACAG 
CCTGCTCCTC CCACGTCGAT CAAACCCAAC ATCGTGGTAC TTAACGATAG TGTGGTCGAA 
TCCTCAGCGG CTTTTCGGCT TCACAACTGC ACTTTGTGCA CTGCCAAATT TATAACAATA 
GATAGCTTAA ATCAGCATTA TGCACACAGT CACAGTAATT CCACTCCCAT GGTTAGTGTT 
CCTCCGATGA ATATATGCCT TCCCAGCTTA ACCATGAACC CTCCAATGAA AATGGAAAGC 
AACAGAGGTC TTGTGGCCAC TGAACAATCC GAATACTGGC CGTTGGAGTG GCATAATAGT 
CCTGTATCCA AGCAACCTAG GAAAAAGATT GATATACTAG ATATGCAGAG TAGTTTCCTG 
CATCACAAAG ACCTTATACC GACAGCCACT CAACTGTCAG CACCCGCACC TGATCCTCAA 
CTGGTTGCAG TGACTGCTAG CTACGCTAAA CTGGCTAACA GGTATCGTAA GTTGCAGCTG 
AAATGCAAAA AACTGAAGTC ACCAAGGAAG GTGCACAGAA AAACCTATGT GTGCCGGATG 
TGCAGGAAGG GATTTTCAAA GTTTAAGAAC TTACACCACC ACCGTCGTCA GAAAGCACAC 
TTTGTAAAGT TAATACCCAA TTTTTCAGGA CGCTGTTCTG GTTGCCTAAA GTTTTTTCGA 
TCCCGCTTGG GTCTTCGCCA GCATATGCGA TACATATGTC AATCGTTGTC GCTCAAAAAC 
CATCGGCGCC TTCAGAGCTT CAAGTGTCGC CACTGCCAGG CCATCGCCTT TGCCCACTGG 
CGCCTGTATC GTCGTCATGA ACTCAATTGC AGGCCCAAAA AATCCAAAAC TAAGGTGCAG 
ACGGCAATGA ATTCGAAAAA GAAAGTTACG CCCACCCAAG TATTTGAGTG TAATATTTGT 
AAAAAATCAT TCGGTTCGCT CAACGGACTA CGCCAGCATA ACATCACCCA CTCGACGGAG 
CGGCAACACA AGTGCGGCAT CTGTGAGCGG GTCTTCAAGC GACGCAACGG ATTGTCTCAA 
CACATTAAGG GCTACCACCT GCAGCTCAAG CCGCATGAGT GTCCGGTTTG CCAGCATCGC 
TACGCCCTGA AATGCGATAT GCTGAGGTGC AGGCATTCCC TGCGAAAAGG TCCAGCTGCC 
GGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609924.2
CDS	68..2356
Translation	MPAHCAVINCSHKYVHAGSISFHRFPFKRKDLLQKWKEFTQRSAQWMPSKWSALCSRHFGDEDFNCSNNRKTLKKNAVPSIRVSEDDSMSGHLEQVSPSNRPTHKQTLSSAAESAATGVKATCRFCGSSAINCSSFDKSFELFGMIQKCFPTLQILQDDTLPKEICQECCLQLERFSQFIDVVMLAQSELQRKYRRQLKIKQEPLVRVKQEACESLDNLFPDELDMGLEQDDGCDQEETEQKFQFCEFPMLNAQDIINNCDIMEIINLDDPFINIPDDADVGQSERLQPGRTANEMLQNELLTEEHNYAKEEWQLPPHQYKTEKVEGTDAAPDAAPPPADPPPLQDNIDPPSAPEPRSCKPIVTNVSQIPNPMICELLPQPAPPTSIKPNIVVLNDSVVESSAAFRLHNCTLCTAKFITIDSLNQHYAHSHSNSTPMVSVPPMNICLPSLTMNPPMKMESNRGLVATEQSEYWPLEWHNSPVSKQPRKKIDILDMQSSFLHHKDLIPTATQLSAPAPDPQLVAVTASYAKLANRYRKLQLKCKKLKSPRKVHRKTYVCRMCRKGFSKFKNLHHHRRQKAHFVKLIPNFSGRCSGCLKFFRSRLGLRQHMRYICQSLSLKNHRRLQSFKCRHCQAIAFAHWRLYRRHELNCRPKKSKTKVQTAMNSKKKVTPTQVFECNICKKSFGSLNGLRQHNITHSTERQHKCGICERVFKRRNGLSQHIKGYHLQLKPHECPVCQHRYALKCDMLRCRHSLRKGPAAGE

Target ORF information:

RefSeq Version	NM_136080.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG10431), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136080.3

ORF Insert Sequence:

ATGCCCGCCC ACTGCGCCGT GATAAATTGC AGCCATAAGT ATGTGCACGC GGGCAGCATT 
TCATTTCACA GATTTCCATT CAAACGGAAG GATCTTCTAC AGAAATGGAA GGAATTTACC 
CAGAGGAGTG CTCAGTGGAT GCCCTCAAAA TGGAGTGCGC TGTGCAGTCG GCACTTTGGT 
GATGAGGACT TTAATTGCAG CAATAATCGG AAAACGCTTA AGAAAAATGC TGTGCCATCA 
ATTAGGGTTT CGGAAGATGA CTCCATGAGT GGGCACTTGG AACAGGTGTC CCCAAGCAAT 
AGGCCAACGC ACAAGCAAAC TCTTTCTAGT GCCGCCGAAT CAGCAGCAAC AGGTGTTAAA 
GCCACTTGCC GCTTTTGTGG TTCCTCGGCC ATCAATTGCT CCTCGTTTGA CAAGAGTTTT 
GAGCTCTTTG GCATGATCCA GAAGTGCTTT CCTACGCTAC AGATTCTTCA GGACGACACC 
TTGCCCAAGG AAATATGCCA GGAGTGCTGC CTCCAATTGG AGCGCTTTTC CCAGTTCATC 
GATGTGGTGA TGTTGGCGCA GAGTGAATTG CAAAGAAAAT ACCGCCGACA ACTGAAAATC 
AAACAGGAAC CCTTGGTTCG TGTGAAACAG GAGGCCTGCG AGAGCTTGGA CAATCTTTTT 
CCTGATGAAC TGGACATGGG TTTGGAGCAG GACGATGGCT GCGATCAGGA AGAAACTGAA 
CAAAAGTTTC AGTTCTGCGA GTTCCCTATG CTAAATGCCC AAGACATTAT CAACAATTGT 
GATATCATGG AGATAATCAA TCTGGACGAT CCGTTTATCA ATATTCCAGA CGATGCGGAT 
GTGGGACAGT CGGAGAGACT ACAGCCTGGA AGAACGGCTA ATGAAATGCT GCAAAACGAA 
CTCCTAACCG AAGAGCACAA TTATGCGAAG GAGGAGTGGC AACTACCCCC GCATCAGTAC 
AAAACGGAAA AGGTGGAGGG AACGGATGCT GCGCCAGACG CAGCTCCTCC GCCTGCTGAT 
CCCCCTCCTC TTCAGGATAA TATAGACCCT CCCTCGGCCC CCGAACCGCG CTCATGCAAG 
CCCATTGTGA CCAATGTAAG CCAGATACCT AACCCAATGA TCTGCGAGCT GCTGCCACAG 
CCTGCTCCTC CCACGTCGAT CAAACCCAAC ATCGTGGTAC TTAACGATAG TGTGGTCGAA 
TCCTCAGCGG CTTTTCGGCT TCACAACTGC ACTTTGTGCA CTGCCAAATT TATAACAATA 
GATAGCTTAA ATCAGCATTA TGCACACAGT CACAGTAATT CCACTCCCAT GGTTAGTGTT 
CCTCCGATGA ATATATGCCT TCCCAGCTTA ACCATGAACC CTCCAATGAA AATGGAAAGC 
AACAGAGGTC TTGTGGCCAC TGAACAATCC GAATACTGGC CGTTGGAGTG GCATAATAGT 
CCTGTATCCA AGCAACCTAG GAAAAAGATT GATATACTAG ATATGCAGAG TAGTTTCCTG 
CATCACAAAG ACCTTATACC GACAGCCACT CAACTGTCAG CACCCGCACC TGATCCTCAA 
CTGGTTGCAG TGACTGCTAG CTACGCTAAA CTGGCTAACA GGTATCGTAA GTTGCAGCTG 
AAATGCAAAA AACTGAAGTC ACCAAGGAAG GTGCACAGAA AAACCTATGT GTGCCGGATG 
TGCAGGAAGG GATTTTCAAA GTTTAAGAAC TTACACCACC ACCGTCGTCA GAAAGCACAC 
TTTGTAAAGT TAATACCCAA TTTTTCAGGA CGCTGTTCTG GTTGCCTAAA GTTTTTTCGA 
TCCCGCTTGG GTCTTCGCCA GCATATGCGA TACATATGTC AATCGTTGTC GCTCAAAAAC 
CATCGGCGCC TTCAGAGCTT CAAGTGTCGC CACTGCCAGG CCATCGCCTT TGCCCACTGG 
CGCCTGTATC GTCGTCATGA ACTCAATTGC AGGCCCAAAA AATCCAAAAC TAAGGTGCAG 
ACGGCAATGA ATTCGAAAAA GAAAGTTACG CCCACCCAAG TATTTGAGTG TAATATTTGT 
AAAAAATCAT TCGGTTCGCT CAACGGACTA CGCCAGCATA ACATCACCCA CTCGACGGAG 
CGGCAACACA AGTGCGGCAT CTGTGAGCGG GTCTTCAAGC GACGCAACGG ATTGTCTCAA 
CACATTAAGG GCTACCACCT GCAGCTCAAG CCGCATGAGT GTCCGGTTTG CCAGCATCGC 
TACGCCCTGA AATGCGATAT GCTGAGGTGC AGGCATTCCC TGCGAAAAGG TCCAGCTGCC 
GGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG10431 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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