Sos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sos
Entrez Gene ID	34790
Full Name	Son of sevenless
Synonyms	2.3,34Ea,BG:DS00941.4,CG7793,Dmel\CG7793,E(sev)2A,E(var)189,EC2-5,EK2-8,EY2-3,SOS,Sos1,Su(tor)2-2,br24,br25,dSos,dme-SOS,l(2)34Ea,l(2)Sos,l(2)br24,l(2)br25,l(2)k05224,l(2)k06321,sos
General protein information	Preferred Names Son of sevenless Names CG7793 gene product from transcript CG7793-RA CG7793-PA Son-of-sevenless Sos-PA lethal 24 in the black-reduced region lethal 25 in the black-reduced region
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34D1-34D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057249.6	NP_476597.2	Son of sevenless

Pathways from BioSystems

Homologies

	SOS1	XP_001103238.2
Drosophila melanogaster (fruit fly)	Sos	NP_476597.2
Canis lupus familiaris (dog)	SOS1	XP_005630658.1
Mus musculus (house mouse)	Sos1	NP_033257.2
Caenorhabditis elegans (roundworm)	sos-1	NP_504235.2
Xenopus tropicalis (tropical clawed frog)	sos1	XP_004914729.1
Pan troglodytes (chimpanzee)	SOS1	XP_515425.3
Bos taurus (cattle)	SOS1	XP_002691287.2
Gallus gallus (chicken)	SOS1	XP_003640979.2
Homo sapiens (human)	SOS1	NP_005624.2
Rattus norvegicus (Norway rat)	Sos1	NP_001094186.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data support a model in which Robo's ligand-dependent internalization from cell surface to late endosome is essential for receptor activation and proper repulsive guidance at the midline by allowing recruitment of the downstream effector Son of Sevenless
Title: Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion.

identifies DRK/DOS/SOS as the upstream Rac GEF complex required for glial responses to axonal injury, and demonstrates a critical requirement for multiple GEFs in efficient glial activation after injury and internalization/degradation of axonal debris
Title: DRK/DOS/SOS converge with Crk/Mbc/dCed-12 to activate Rac1 during glial engulfment of axonal debris.

These results provide in vivo evidence that the Ras and RhoGEF domains of Sos are separable signaling modules and support a model in which Robo recruits Sos to the membrane via Dock to activate Rac during midline repulsion.
Title: Son of sevenless directly links the Robo receptor to rac activation to control axon repulsion at the midline.

SosM98, an allele with a single amino acid substitution in the RacGEF motif, functions as a dominant negative in vivo to downregulate receptor tyrosine kinase signaling.
Title: New class of Son-of-sevenless (Sos) alleles highlights the complexities of Sos function.

Signalling from the pTyr (YB) Neu (ErbB-2) autophosphorylation site was affected by a haplosufficiency in drk (Grb-2), and in genes thought to function downstream of Grb-2: dab, sos, csw (Shp-2), and dos (Gab-1).
Title: Genetic identification of effectors downstream of Neu (ErbB-2) autophosphorylation sites in a Drosophila model.

The following Sos gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sos cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16730	NM_057249.6 Latest version!	Drosophila melanogaster Son of sevenless (Sos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sos ( NM_057249.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476597.2 Drosophila melanogaster Son of sevenless (Sos), mRNA.

Price & Availability↑

CloneID	OFa16730
Clone ID Related Accession (Same CDS sequence)	NM_057249.6
Accession Version	NM_057249.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Son of sevenless
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Oct 19, 2015 this sequence version replaced NM_057249.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTCGG GGCCCAGCGG CCATGCCCAC ACCATTAGCT ACGGGGGCGG GATCGGTCTG 
GGCACTGGAG GCGGCGGCGG CAGCGGTGGT AGTGGTAGTG GCTCACAGGG CGGAGGCGGT 
GGTATTGGCA TTGGCGGCGG TGGCGTGGCT GGGCTGCAGG ATTGCGATGG CTATGACTTC 
ACCAAGTGCG AGAATGCTGC GAGATGGAGG GGCTTGTTTA CGCCATCGCT AAAGAAGGTG 
CTGGAGCAAG TGCATCCACG GGTTACCGCC AAGGAGGACG CACTGCTGTA TGTGGAGAAA 
CTCTGTCTGC GGCTTCTGGC CATGCTGTGC GCCAAGCCGC TGCCCCATTC CGTGCAGGAT 
GTGGAGGAGA AGGTGAACAA GTCCTTTCCA GCGCCCATCG ATCAGTGGGC CCTGAACGAG 
GCCAAGGAGG TGATCAACTC GAAGAAACGC AAGTCTGTCC TGCCCACGGA AAAGGTGCAC 
ACGCTGCTCC AGAAGGATGT GTTGCAGTAC AAGATCGATA GCTCCGTGTC CGCCTTCCTG 
GTGGCCGTGC TCGAGTACAT CTCAGCGGAC ATACTCAAAA TGGCCGGCGA CTATGTGATC 
AAGATCGCCC ACTGCGAGAT CACCAAGGAG GACATCGAGG TGGTGATGAA CGCCGATCGT 
GTGCTAATGG ACATGCTCAA CCAGAGCGAA GCCCACATCC TGCCCAGTCC CCTGTCACTT 
CCCGCGCAGC GTGCAAGTGC CACCTACGAG GAGACGGTCA AGGAGCTGAT CCACGACGAG 
AAGCAGTACC AACGCGACCT GCACATGATC ATACGCGTCT TTCGTGAGGA GCTGGTGAAG 
ATCGTGTCCG ATCCGCGCGA GCTGGAACCG ATATTCTCCA ACATAATGGA CATTTACGAG 
GTGACGGTCA CTCTGCTCGG CTCCCTGGAG GACGTCATCG AGATGTCCCA GGAGCAGAGT 
GCCCCCTGCG TCGGTAGCTG CTTTGAGGAA CTGGCCGAGG CCGAGGAGTT CGATGTGTAC 
AAGAAGTACG CCTACGACGT TACCTCGCAG GCCTCACGGG ATGCTCTCAA CAATCTCCTG 
TCTAAGCCAG GGGCCTCATC TCTGACCACA GCCGGCCATG GCTTCCGCGA TGCGGTCAAA 
TACTATTTGC CCAAGCTGCT TCTGGTGCCC ATTTGCCATG CCTTCGTGTA TTTCGACTAC 
ATCAAGCATC TCAAGGATCT CAGCTCTTCG CAGGACGACA TCGAGAGCTT CGAACAGGTA 
CAGGGACTGT TGCATCCACT CCACTGCGAT CTCGAAAAGG TAATGGCCAG CCTGTCCAAG 
GAGCGACAAG TTCCGGTTAG CGGTCGAGTG CGCCGCCAGC TGGCAATCGA GCGGACACGG 
GAGCTCCAAA TGAAGGTGGA GCACTGGGAG GACAAGGACG TGGGCCAGAA TTGCAATGAA 
TTTATTCGCG AGGATTCGCT GAGCAAACTT GGATCGGGAA AACGAATCTG GAGCGAGCGC 
AAGGTATTCC TCTTCGACGG GCTAATGGTG CTATGCAAGG CAAACACCAA GAAACAAACA 
CCATCGGCAG GAGCAACGGC CTACGACTAC CGACTGAAGG AGAAGTATTT CATGCGACGC 
GTGGATATCA ACGATCGACC GGACAGCGAT GATCTGAAGA ACAGTTTTGA GTTGGCACCC 
CGGATGCAGC CGCCCATTGT GCTGACCGCC AAGAATGCAC AGCACAAGCA CGACTGGATG 
GCAGACCTGC TGATGGTTAT CACCAAGTCG ATGCTGGACC GGCACTTGGA CAGCATATTG 
CAAGACATCG AGCGAAAGCA TCCGCTGCGG ATGCCCAGTC CGGAGATTTA CAAGTTCGCG 
GTGCCGGACA GCGGTGACAA TATCGTGTTG GAGGAGCGCG AAAGCGCAGG AGTGCCGATG 
ATCAAGGGAG CGACGCTTTG CAAGCTAATC GAGCGCCTCA CCTATCACAT CTACGCCGAT 
CCGACTTTTG TGCGCACCTT CCTCACCACA TATCGCTACT TCTGCTCGCC GCAGCAATTG 
CTGCAACTGC TGGTGGAACG CTTTAACATA CCGGATCCCA GCCTGGTCTA TCAAGACACG 
GGCACAGCAG GTGCGGGTGG AATGGGCGGC GTTGGCGGTG ACAAGGAGCA CAAGAACTCG 
CATCGCGAGG ACTGGAAACG ATATCGCAAG GAGTACGTGC AGCCAGTGCA GTTTCGAGTG 
CTCAACGTAC TGCGCCATTG GGTCGACCAT CATTTTTACG ATTTCGAGAA GGATCCCATG 
TTGCTGGAGA AGCTGCTGAA CTTTCTGGAG CACGTGAATG GCAAATCGAT GCGCAAGTGG 
GTGGATTCCG TGCTTAAGAT TGTTCAGAGA AAGAACGAGC AGGAGAAAAG CAATAAAAAG 
ATTGTATACG CCTATGGCCA CGATCCGCCG CCCATTGAGC ATCACCTTAG TGTACCCAAC 
GACGAGATAA CGCTCCTCAC CCTGCACCCA CTGGAACTGG CCCGTCAGCT CACTCTACTG 
GAATTCGAGA TGTACAAGAA TGTAAAGCCG TCTGAGTTGG TCGGATCACC CTGGACGAAA 
AAGGACAAGG AGGTGAAGAG CCCTAATCTA CTGAAGATAA TGAAGCACAC CACGAACGTC 
ACCCGCTGGA TTGAAAAATC CATCACCGAA GCGGAGAACT ACGAGGAACG CCTGGCTATT 
ATGCAACGCG CAATCGAAGT GATGATGGTG ATGCTGGAAT TGAACAATTT TAATGGAATC 
CTCTCGATTG TCGCGGCTAT GGGCACGGCA TCTGTTTATC GACTGCGGTG GACATTCCAG 
GGATTGCCCG AACGCTACAG AAAATTCTTG GAGGAATGCC GCGAGCTCAG CGACGATCAT 
CTCAAAAAGT ATCAGGAGCG ATTGCGATCC ATCAATCCGC CTTGTGTGCC ATTTTTCGGT 
CGCTACTTGA CCAACATACT CCACTTGGAG GAGGGTAACC CCGACCTGCT AGCCAACACA 
GAGCTAATTA ACTTTTCCAA ACGACGGAAA GTGGCCGAGA TTATTGGCGA GATTCAACAG 
TACCAGAACC AGCCATACTG CCTCAACGAG GAGTCCACAA TACGACAGTT CTTCGAGCAA 
CTGGATCCGT TTAACGGACT GTCCGACAAA CAGATGTCCG ACTATCTCTA TAACGAAAGC 
CTGCGCATTG AGCCAAGGGG CTGCAAGACA GTGCCGAAAT TCCCTCGAAA ATGGCCGCAC 
ATTCCGCTCA AATCGCCGGG CATCAAGCCG CGTCGCCAGA ATCAGACCAA CAGCAGTAGC 
AAGCTGTCGA ACAGCACGTC GTCCGTGGCG GCGGCAGCGG CAGCATCGTC AACGGCCACC 
TCAATAGCTA CGGCATCGGC GCCATCTTTG CACGCCTCCA GCATAATGGA TGCGCCAACA 
GCAGCAGCAG CTAATGCTGG ATCTGGAACT CTCGCTGGCG AGCAAAGTCC GCAGCACAAT 
CCGCACGCTT TCTCCGTTTT CGCCCCTGTT ATTATACCCG AACGGAACAC CAGCAGCTGG 
AGTGGAACGC CACAGCACAC TCGAACGGAC CAGAACAACG GGGAGGTTTC GGTGCCGGCG 
CCACATCTCC CCAAGAAACC GGGCGCGCAT GTCTGGGCTA ACAACAACTC GACACTGGCC 
AGTGCGTCGG CAATGGATGT TGTGTTCAGT CCAGCGCTGC CGGAGCATCT GCCACCGCAG 
TCCCTGCCGG ACAGCAATCC ATTCGCATCG GACACGGAAG CTCCACCGTC GCCGCTGCCC 
AAGCTAGTGG TCAGTCCGCG TCACGAAACC GGCAATCGAT CACCATTCCA TGGGCGCATG 
CAGAACAGCC CAACGCATAG CACTGCCAGT ACCGTGACCC TTACAGGCAT GTCTACATCG 
GGCGGGGAGG AATTCTGCGC GGGTGGATTC TACTTTAACA GTGCCCATCA GGGACAGCCG 
GGGGCAGTGC CCATCTCGCC GCATGTCAAC GTTCCGATGG CCACCAATAT GGAGTACCGA 
GCAGTGCCGC CTCCACTGCC ACCCAGACGC AAGGAGCGCA CTGAGAGCTG TGCGGACATG 
GCGCAAAAGC GTCAGGCGCC AGACGCACCC ACATTACCCC CGCGTGATGG CGAACTCAGT 
CCGCCGCCGA TACCGCCACG GCTCAACCAT TCCACGGGCA TCAGCTACTT GCGACAAAGC 
CACGGCAAGA GCAAGGAGTT TGTGGGCAAC AGCAGTCTGC TCCTGCCCAA CACCAGCAGT 
ATTATGATAC GCCGCAACTC GGCGATCGAG AAGCGGGCAG CGGCAACTAG CCAGCCAAAC 
CAGGCGGCGG CGGGACCCAT CAGCACGACA CTAGTGACCG TGTCGCAGGC AGTGGCGACG 
GACGAACCGC TGCCGCTACC GATCTCGCCA GCGGCAAGCT CCTCGACGAC CACATCACCG 
CTGACACCCG CCATGTCGCC CATGTCTCCC AACATTCCCA GCCATCCGGT GGAGAGCACG 
TCGAGCAGCT ATGCCCACCA ACTGCGAATG CGGCAGCAGC AGCAGCAGCA GACGCATCCA 
GCGATCTACT CGCAGCACCA TCAACATCAT GCCACCCATC TGCCGCACCA TCCGCACCAG 
CACCATTCGA ATCCGACGCA GTCGCGCTCG TCCCCCAAGG AGTTCTTTCC GATTGCCACG 
AGCCTCGAGG GCACACCCAA ACTTCCACCA AAACCTAGTC TAAGCGCTAA CTTCTATAAC 
AATCCAGATA AAGGTACGAT GTTTCTTTAC CCAAGTACAA ACGAAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476597.2
CDS	399..5189
Translation	MFSGPSGHAHTISYGGGIGLGTGGGGGSGGSGSGSQGGGGGIGIGGGGVAGLQDCDGYDFTKCENAARWRGLFTPSLKKVLEQVHPRVTAKEDALLYVEKLCLRLLAMLCAKPLPHSVQDVEEKVNKSFPAPIDQWALNEAKEVINSKKRKSVLPTEKVHTLLQKDVLQYKIDSSVSAFLVAVLEYISADILKMAGDYVIKIAHCEITKEDIEVVMNADRVLMDMLNQSEAHILPSPLSLPAQRASATYEETVKELIHDEKQYQRDLHMIIRVFREELVKIVSDPRELEPIFSNIMDIYEVTVTLLGSLEDVIEMSQEQSAPCVGSCFEELAEAEEFDVYKKYAYDVTSQASRDALNNLLSKPGASSLTTAGHGFRDAVKYYLPKLLLVPICHAFVYFDYIKHLKDLSSSQDDIESFEQVQGLLHPLHCDLEKVMASLSKERQVPVSGRVRRQLAIERTRELQMKVEHWEDKDVGQNCNEFIREDSLSKLGSGKRIWSERKVFLFDGLMVLCKANTKKQTPSAGATAYDYRLKEKYFMRRVDINDRPDSDDLKNSFELAPRMQPPIVLTAKNAQHKHDWMADLLMVITKSMLDRHLDSILQDIERKHPLRMPSPEIYKFAVPDSGDNIVLEERESAGVPMIKGATLCKLIERLTYHIYADPTFVRTFLTTYRYFCSPQQLLQLLVERFNIPDPSLVYQDTGTAGAGGMGGVGGDKEHKNSHREDWKRYRKEYVQPVQFRVLNVLRHWVDHHFYDFEKDPMLLEKLLNFLEHVNGKSMRKWVDSVLKIVQRKNEQEKSNKKIVYAYGHDPPPIEHHLSVPNDEITLLTLHPLELARQLTLLEFEMYKNVKPSELVGSPWTKKDKEVKSPNLLKIMKHTTNVTRWIEKSITEAENYEERLAIMQRAIEVMMVMLELNNFNGILSIVAAMGTASVYRLRWTFQGLPERYRKFLEECRELSDDHLKKYQERLRSINPPCVPFFGRYLTNILHLEEGNPDLLANTELINFSKRRKVAEIIGEIQQYQNQPYCLNEESTIRQFFEQLDPFNGLSDKQMSDYLYNESLRIEPRGCKTVPKFPRKWPHIPLKSPGIKPRRQNQTNSSSKLSNSTSSVAAAAAASSTATSIATASAPSLHASSIMDAPTAAAANAGSGTLAGEQSPQHNPHAFSVFAPVIIPERNTSSWSGTPQHTRTDQNNGEVSVPAPHLPKKPGAHVWANNNSTLASASAMDVVFSPALPEHLPPQSLPDSNPFASDTEAPPSPLPKLVVSPRHETGNRSPFHGRMQNSPTHSTASTVTLTGMSTSGGEEFCAGGFYFNSAHQGQPGAVPISPHVNVPMATNMEYRAVPPPLPPRRKERTESCADMAQKRQAPDAPTLPPRDGELSPPPIPPRLNHSTGISYLRQSHGKSKEFVGNSSLLLPNTSSIMIRRNSAIEKRAAATSQPNQAAAGPISTTLVTVSQAVATDEPLPLPISPAASSSTTTSPLTPAMSPMSPNIPSHPVESTSSSYAHQLRMRQQQQQQTHPAIYSQHHQHHATHLPHHPHQHHSNPTQSRSSPKEFFPIATSLEGTPKLPPKPSLSANFYNNPDKGTMFLYPSTNEE

Target ORF information:

RefSeq Version	NM_057249.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Son of sevenless (Sos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057249.6

ORF Insert Sequence:

ATGTTCTCGG GGCCCAGCGG CCATGCCCAC ACCATTAGCT ACGGGGGCGG GATCGGTCTG 
GGCACTGGAG GCGGCGGCGG CAGCGGTGGT AGTGGTAGTG GCTCACAGGG CGGAGGCGGT 
GGTATTGGCA TTGGCGGCGG TGGCGTGGCT GGGCTGCAGG ATTGCGATGG CTATGACTTC 
ACCAAGTGCG AGAATGCTGC GAGATGGAGG GGCTTGTTTA CGCCATCGCT AAAGAAGGTG 
CTGGAGCAAG TGCATCCACG GGTTACCGCC AAGGAGGACG CACTGCTGTA TGTGGAGAAA 
CTCTGTCTGC GGCTTCTGGC CATGCTGTGC GCCAAGCCGC TGCCCCATTC CGTGCAGGAT 
GTGGAGGAGA AGGTGAACAA GTCCTTTCCA GCGCCCATCG ATCAGTGGGC CCTGAACGAG 
GCCAAGGAGG TGATCAACTC GAAGAAACGC AAGTCTGTCC TGCCCACGGA AAAGGTGCAC 
ACGCTGCTCC AGAAGGATGT GTTGCAGTAC AAGATCGATA GCTCCGTGTC CGCCTTCCTG 
GTGGCCGTGC TCGAGTACAT CTCAGCGGAC ATACTCAAAA TGGCCGGCGA CTATGTGATC 
AAGATCGCCC ACTGCGAGAT CACCAAGGAG GACATCGAGG TGGTGATGAA CGCCGATCGT 
GTGCTAATGG ACATGCTCAA CCAGAGCGAA GCCCACATCC TGCCCAGTCC CCTGTCACTT 
CCCGCGCAGC GTGCAAGTGC CACCTACGAG GAGACGGTCA AGGAGCTGAT CCACGACGAG 
AAGCAGTACC AACGCGACCT GCACATGATC ATACGCGTCT TTCGTGAGGA GCTGGTGAAG 
ATCGTGTCCG ATCCGCGCGA GCTGGAACCG ATATTCTCCA ACATAATGGA CATTTACGAG 
GTGACGGTCA CTCTGCTCGG CTCCCTGGAG GACGTCATCG AGATGTCCCA GGAGCAGAGT 
GCCCCCTGCG TCGGTAGCTG CTTTGAGGAA CTGGCCGAGG CCGAGGAGTT CGATGTGTAC 
AAGAAGTACG CCTACGACGT TACCTCGCAG GCCTCACGGG ATGCTCTCAA CAATCTCCTG 
TCTAAGCCAG GGGCCTCATC TCTGACCACA GCCGGCCATG GCTTCCGCGA TGCGGTCAAA 
TACTATTTGC CCAAGCTGCT TCTGGTGCCC ATTTGCCATG CCTTCGTGTA TTTCGACTAC 
ATCAAGCATC TCAAGGATCT CAGCTCTTCG CAGGACGACA TCGAGAGCTT CGAACAGGTA 
CAGGGACTGT TGCATCCACT CCACTGCGAT CTCGAAAAGG TAATGGCCAG CCTGTCCAAG 
GAGCGACAAG TTCCGGTTAG CGGTCGAGTG CGCCGCCAGC TGGCAATCGA GCGGACACGG 
GAGCTCCAAA TGAAGGTGGA GCACTGGGAG GACAAGGACG TGGGCCAGAA TTGCAATGAA 
TTTATTCGCG AGGATTCGCT GAGCAAACTT GGATCGGGAA AACGAATCTG GAGCGAGCGC 
AAGGTATTCC TCTTCGACGG GCTAATGGTG CTATGCAAGG CAAACACCAA GAAACAAACA 
CCATCGGCAG GAGCAACGGC CTACGACTAC CGACTGAAGG AGAAGTATTT CATGCGACGC 
GTGGATATCA ACGATCGACC GGACAGCGAT GATCTGAAGA ACAGTTTTGA GTTGGCACCC 
CGGATGCAGC CGCCCATTGT GCTGACCGCC AAGAATGCAC AGCACAAGCA CGACTGGATG 
GCAGACCTGC TGATGGTTAT CACCAAGTCG ATGCTGGACC GGCACTTGGA CAGCATATTG 
CAAGACATCG AGCGAAAGCA TCCGCTGCGG ATGCCCAGTC CGGAGATTTA CAAGTTCGCG 
GTGCCGGACA GCGGTGACAA TATCGTGTTG GAGGAGCGCG AAAGCGCAGG AGTGCCGATG 
ATCAAGGGAG CGACGCTTTG CAAGCTAATC GAGCGCCTCA CCTATCACAT CTACGCCGAT 
CCGACTTTTG TGCGCACCTT CCTCACCACA TATCGCTACT TCTGCTCGCC GCAGCAATTG 
CTGCAACTGC TGGTGGAACG CTTTAACATA CCGGATCCCA GCCTGGTCTA TCAAGACACG 
GGCACAGCAG GTGCGGGTGG AATGGGCGGC GTTGGCGGTG ACAAGGAGCA CAAGAACTCG 
CATCGCGAGG ACTGGAAACG ATATCGCAAG GAGTACGTGC AGCCAGTGCA GTTTCGAGTG 
CTCAACGTAC TGCGCCATTG GGTCGACCAT CATTTTTACG ATTTCGAGAA GGATCCCATG 
TTGCTGGAGA AGCTGCTGAA CTTTCTGGAG CACGTGAATG GCAAATCGAT GCGCAAGTGG 
GTGGATTCCG TGCTTAAGAT TGTTCAGAGA AAGAACGAGC AGGAGAAAAG CAATAAAAAG 
ATTGTATACG CCTATGGCCA CGATCCGCCG CCCATTGAGC ATCACCTTAG TGTACCCAAC 
GACGAGATAA CGCTCCTCAC CCTGCACCCA CTGGAACTGG CCCGTCAGCT CACTCTACTG 
GAATTCGAGA TGTACAAGAA TGTAAAGCCG TCTGAGTTGG TCGGATCACC CTGGACGAAA 
AAGGACAAGG AGGTGAAGAG CCCTAATCTA CTGAAGATAA TGAAGCACAC CACGAACGTC 
ACCCGCTGGA TTGAAAAATC CATCACCGAA GCGGAGAACT ACGAGGAACG CCTGGCTATT 
ATGCAACGCG CAATCGAAGT GATGATGGTG ATGCTGGAAT TGAACAATTT TAATGGAATC 
CTCTCGATTG TCGCGGCTAT GGGCACGGCA TCTGTTTATC GACTGCGGTG GACATTCCAG 
GGATTGCCCG AACGCTACAG AAAATTCTTG GAGGAATGCC GCGAGCTCAG CGACGATCAT 
CTCAAAAAGT ATCAGGAGCG ATTGCGATCC ATCAATCCGC CTTGTGTGCC ATTTTTCGGT 
CGCTACTTGA CCAACATACT CCACTTGGAG GAGGGTAACC CCGACCTGCT AGCCAACACA 
GAGCTAATTA ACTTTTCCAA ACGACGGAAA GTGGCCGAGA TTATTGGCGA GATTCAACAG 
TACCAGAACC AGCCATACTG CCTCAACGAG GAGTCCACAA TACGACAGTT CTTCGAGCAA 
CTGGATCCGT TTAACGGACT GTCCGACAAA CAGATGTCCG ACTATCTCTA TAACGAAAGC 
CTGCGCATTG AGCCAAGGGG CTGCAAGACA GTGCCGAAAT TCCCTCGAAA ATGGCCGCAC 
ATTCCGCTCA AATCGCCGGG CATCAAGCCG CGTCGCCAGA ATCAGACCAA CAGCAGTAGC 
AAGCTGTCGA ACAGCACGTC GTCCGTGGCG GCGGCAGCGG CAGCATCGTC AACGGCCACC 
TCAATAGCTA CGGCATCGGC GCCATCTTTG CACGCCTCCA GCATAATGGA TGCGCCAACA 
GCAGCAGCAG CTAATGCTGG ATCTGGAACT CTCGCTGGCG AGCAAAGTCC GCAGCACAAT 
CCGCACGCTT TCTCCGTTTT CGCCCCTGTT ATTATACCCG AACGGAACAC CAGCAGCTGG 
AGTGGAACGC CACAGCACAC TCGAACGGAC CAGAACAACG GGGAGGTTTC GGTGCCGGCG 
CCACATCTCC CCAAGAAACC GGGCGCGCAT GTCTGGGCTA ACAACAACTC GACACTGGCC 
AGTGCGTCGG CAATGGATGT TGTGTTCAGT CCAGCGCTGC CGGAGCATCT GCCACCGCAG 
TCCCTGCCGG ACAGCAATCC ATTCGCATCG GACACGGAAG CTCCACCGTC GCCGCTGCCC 
AAGCTAGTGG TCAGTCCGCG TCACGAAACC GGCAATCGAT CACCATTCCA TGGGCGCATG 
CAGAACAGCC CAACGCATAG CACTGCCAGT ACCGTGACCC TTACAGGCAT GTCTACATCG 
GGCGGGGAGG AATTCTGCGC GGGTGGATTC TACTTTAACA GTGCCCATCA GGGACAGCCG 
GGGGCAGTGC CCATCTCGCC GCATGTCAAC GTTCCGATGG CCACCAATAT GGAGTACCGA 
GCAGTGCCGC CTCCACTGCC ACCCAGACGC AAGGAGCGCA CTGAGAGCTG TGCGGACATG 
GCGCAAAAGC GTCAGGCGCC AGACGCACCC ACATTACCCC CGCGTGATGG CGAACTCAGT 
CCGCCGCCGA TACCGCCACG GCTCAACCAT TCCACGGGCA TCAGCTACTT GCGACAAAGC 
CACGGCAAGA GCAAGGAGTT TGTGGGCAAC AGCAGTCTGC TCCTGCCCAA CACCAGCAGT 
ATTATGATAC GCCGCAACTC GGCGATCGAG AAGCGGGCAG CGGCAACTAG CCAGCCAAAC 
CAGGCGGCGG CGGGACCCAT CAGCACGACA CTAGTGACCG TGTCGCAGGC AGTGGCGACG 
GACGAACCGC TGCCGCTACC GATCTCGCCA GCGGCAAGCT CCTCGACGAC CACATCACCG 
CTGACACCCG CCATGTCGCC CATGTCTCCC AACATTCCCA GCCATCCGGT GGAGAGCACG 
TCGAGCAGCT ATGCCCACCA ACTGCGAATG CGGCAGCAGC AGCAGCAGCA GACGCATCCA 
GCGATCTACT CGCAGCACCA TCAACATCAT GCCACCCATC TGCCGCACCA TCCGCACCAG 
CACCATTCGA ATCCGACGCA GTCGCGCTCG TCCCCCAAGG AGTTCTTTCC GATTGCCACG 
AGCCTCGAGG GCACACCCAA ACTTCCACCA AAACCTAGTC TAAGCGCTAA CTTCTATAAC 
AATCCAGATA AAGGTACGAT GTTTCTTTAC CCAAGTACAA ACGAAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sos cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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