Edem2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Edem2
Entrez Gene ID	34714
Full Name	ER degradation enhancer, mannosidase alpha-like 2
Synonyms	CG5682,Dmel\CG5682,EDEM2,edem2
General protein information	Preferred Names ER degradation enhancer, mannosidase alpha-like 2 Names CG5682 gene product from transcript CG5682-RA CG5682-PA CG5682-PB Edem2-PA Edem2-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34A8-34A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273505.1	NP_001260434.1	ER degradation enhancer, mannosidase alpha-like 2, isoform B
NM_135767.2	NP_609611.1	ER degradation enhancer, mannosidase alpha-like 2, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Edem3	NP_001034733.2
Rattus norvegicus (Norway rat)	Edem3	NP_001178600.1
Danio rerio (zebrafish)	edem3	XP_005166989.1
Pan troglodytes (chimpanzee)	EDEM3	XP_003308683.1
Caenorhabditis elegans (roundworm)	ZC506.1	NP_509671.3
Xenopus tropicalis (tropical clawed frog)	edem3	XP_002932190.2
Homo sapiens (human)	EDEM3	NP_079467.3
	EDEM3	XP_002802060.1
Canis lupus familiaris (dog)	EDEM3	XP_005622487.1
Bos taurus (cattle)	EDEM3	NP_001192782.1
Gallus gallus (chicken)	EDEM3	XP_422293.4
Drosophila melanogaster (fruit fly)	Edem2	NP_609611.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008749	XP_314865.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Edem2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Edem2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14606	NM_135767.2 Latest version!	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 2 (Edem2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16709	NM_001273505.1 Latest version!	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 2 (Edem2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Edem2 ( NM_135767.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609611.1 Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 2 (Edem2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14606
Clone ID Related Accession (Same CDS sequence)	NM_135767.2
Accession Version	NM_135767.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ER degradation enhancer, mannosidase alpha-like 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135767.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTACCA CATCCTCCGA ATCCTTTGTC CGCGGCCAGC GGCTTATGCT CATCTTCCTG 
GTGGCCATGT GCATCGTAGT TGCCTGCGTC CTTAGCACCG AAACCACCAT GCCCACGCAG 
AACATGTCCA ATAAGGAGCG GGCGGAACTC CGGGAAGAGG CTCGCGATAT GTTCTATCAT 
GCATACAACG CCTACATGCA GAACGCCTAT CCAGCTGATG AGCTTATGCC GCTCTCCTGC 
AAAGGACGGT ACCGAGGAGT GACGCCATCA CGTGGCGACA TGGACGACAT CCTTGGAAAT 
TTCTCCATGA CTCTGGTGGA TACTCTGGAC ACTCTGGTGC TCCTGGGCGA TTTCACAGAG 
TTTGATCACG CGGTGAAGCT AGTTATCCGC GACGTTCAAT TCGACAGCGA CATTATTGTG 
TCGGTGTTTG AGACGAACAT TCGAATGGTC GGTGGCTTGC TGTCGGCGCA CATCCTGGCG 
GAGTATCTGC AAAAGCATGC GGACACGATG CATTGGTACA AGGGCGAACT GCTGGAGATG 
TCTCGCGAGC TGGGCTACCG TTTGCTACCG GCGTTCAACA CATCCACGGG CATTCCGCAT 
GCGCGGGTTA ATCTCCGCCT GGGCATGAAG GACCCGATGC TCAAGAAGTC CCGTGAAACG 
TGTACGGCGT GTGCTGGTAC CATCCTTCTG GAATTTGCTG CTCTGTCGCG TCTCACCGGC 
GATCCCATTT TCGAGGTGCG AGCTCACGCC GCCATGGATG CACTGTGGAA GCTAAGGCAC 
CGTGGCTCCG ATCTCATGGG CACGGTACTT AATGTCCATT CGGGCGATTG GGTGCGTCGC 
GATTCTGGAG TCGGAGCTGG CATCGACAGT TACTATGAGT ACCTGTTCAA GTCGTACGTC 
CTACTGGGTG ACGACAAGTA CCTGGCACGC TTTAATCGCC ACTACAACGC AGTGATGAAG 
TACGTCAGCG AAGGACCCAT GCTACTCGAC GTGCTCATGC ACAGGCCACA TGCTAAGTCC 
AAAAACTTCA TGGACTCGCT TCTGGCATTT TGGCCGGGCT TGCAGGTGCT ATCGGGCGAT 
CTGAAACCAG CTGTGCAGAC ACACGAGATG CTCTATCAGG TCATGCAGAT GCACACCTTC 
ATTCCAGAAG CATTTACCGT CGACTTCCAG ATCCATTGGG GACAGCATCC CCTGCGACCA 
GAATTTATAG AGTCCACTTA CTTCCTGTAC CGCGCCACTG GCGATCATCA TTATCTGCAG 
GTTGGCAAGA AGGCTCTGAA AACACTCCAG CAGCATGCTA AGGTATCTTG CGGCTATGCG 
GCCGTCAATG ATGTACGGAC TGGCAAGCAT GAGGATCGCA TGGACTCGTT TGTGCTCTCC 
GAGACGATCA AGTACCTCTT CCTACTGTTC TCCGATCCCC AGGATCTGAT CATAAACGTC 
GATGAGTTCG TCTTCACGAC CGAGGCTCAT CTGCTACCGC TCTCTATTGC CCAACTAGGG 
AATGCCACGT TTAGCTTTCG CCAGACGGAC GAACACAACG TACTCGACTT CATGCGCACC 
TGCCCCAGTT CAAATAGGCT TTTTCCTGAA AAAGTACGCA AACCACTGCG CAACTTCATT 
ACGGGCTCGT GCCCTCGCAC CACAGCGGGA AAGCGACTCA GCGCCCTGGA TTTTCAGGCA 
AGCAATGCAG ATCATCTTCG AGCTGTTTAC GACATGGGCA TTACCATGGT TTCGGTAGGC 
GACCGCAGCC AAGGCAAGGT GCGACTGTTT CATAGTTTCT ATAATGCCAA AAGTCACGAG 
GAGGGCGAAA TGGGACTGCA ATTTATGCAG GAGATGCTTG AGCTGACCAA GATGCAAAGC 
ATAAATCAGC TAGCGCAACT GCAGGCCGTT GCCTACGCCA CTGACGAGAA CTCACAGGAT 
TGGATCGCAC TGATGGCCGG GCCGTCGCAC TTTAGCCCAG AGTTAACTGG CGATCAGTTC 
GTCGAAGGGG ACGTGATTCT GGCCAAGCCG CTACGAGCAT GCGATGAGAG CCTAGAGAAC 
GCCGAGGAGG CGAAAGGAAA GGTCCTGGTG GCCGAGCGCG GCGATTGCAC GTTCGTTAGC 
AAAGCGCGAC TGGCCCAGAA GGTGGGGGCG GCGGCGCTGA TTGTCTGTGA TAATGTTCCG 
GGCTCTTCCG GTGAGACGCA GCCGATGTTC GCAATGTCTG GCGACGGCAA GGACGACGTG 
CTCATTCCAG TCGTATTCAT GTACAGCATG GAGTTCGGCA AACTGTCGGC GGTCATGCAG 
CGACGGAAAC AACCTCTGCG TGTTCGCGTC ATGCAGATGG TGGAGTTTAA GCGGTGGCAA 
CTGGCCAAGG AGCAGCGCCA GAACCAAACA GCGTCGGTAG CACAGAAACC GGAAAAAGAA 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609611.1
CDS	670..3075
Translation	MSTTSSESFVRGQRLMLIFLVAMCIVVACVLSTETTMPTQNMSNKERAELREEARDMFYHAYNAYMQNAYPADELMPLSCKGRYRGVTPSRGDMDDILGNFSMTLVDTLDTLVLLGDFTEFDHAVKLVIRDVQFDSDIIVSVFETNIRMVGGLLSAHILAEYLQKHADTMHWYKGELLEMSRELGYRLLPAFNTSTGIPHARVNLRLGMKDPMLKKSRETCTACAGTILLEFAALSRLTGDPIFEVRAHAAMDALWKLRHRGSDLMGTVLNVHSGDWVRRDSGVGAGIDSYYEYLFKSYVLLGDDKYLARFNRHYNAVMKYVSEGPMLLDVLMHRPHAKSKNFMDSLLAFWPGLQVLSGDLKPAVQTHEMLYQVMQMHTFIPEAFTVDFQIHWGQHPLRPEFIESTYFLYRATGDHHYLQVGKKALKTLQQHAKVSCGYAAVNDVRTGKHEDRMDSFVLSETIKYLFLLFSDPQDLIINVDEFVFTTEAHLLPLSIAQLGNATFSFRQTDEHNVLDFMRTCPSSNRLFPEKVRKPLRNFITGSCPRTTAGKRLSALDFQASNADHLRAVYDMGITMVSVGDRSQGKVRLFHSFYNAKSHEEGEMGLQFMQEMLELTKMQSINQLAQLQAVAYATDENSQDWIALMAGPSHFSPELTGDQFVEGDVILAKPLRACDESLENAEEAKGKVLVAERGDCTFVSKARLAQKVGAAALIVCDNVPGSSGETQPMFAMSGDGKDDVLIPVVFMYSMEFGKLSAVMQRRKQPLRVRVMQMVEFKRWQLAKEQRQNQTASVAQKPEKEL

Target ORF information:

RefSeq Version	NM_135767.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 2 (Edem2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135767.2

ORF Insert Sequence:

ATGTCTACCA CATCCTCCGA ATCCTTTGTC CGCGGCCAGC GGCTTATGCT CATCTTCCTG 
GTGGCCATGT GCATCGTAGT TGCCTGCGTC CTTAGCACCG AAACCACCAT GCCCACGCAG 
AACATGTCCA ATAAGGAGCG GGCGGAACTC CGGGAAGAGG CTCGCGATAT GTTCTATCAT 
GCATACAACG CCTACATGCA GAACGCCTAT CCAGCTGATG AGCTTATGCC GCTCTCCTGC 
AAAGGACGGT ACCGAGGAGT GACGCCATCA CGTGGCGACA TGGACGACAT CCTTGGAAAT 
TTCTCCATGA CTCTGGTGGA TACTCTGGAC ACTCTGGTGC TCCTGGGCGA TTTCACAGAG 
TTTGATCACG CGGTGAAGCT AGTTATCCGC GACGTTCAAT TCGACAGCGA CATTATTGTG 
TCGGTGTTTG AGACGAACAT TCGAATGGTC GGTGGCTTGC TGTCGGCGCA CATCCTGGCG 
GAGTATCTGC AAAAGCATGC GGACACGATG CATTGGTACA AGGGCGAACT GCTGGAGATG 
TCTCGCGAGC TGGGCTACCG TTTGCTACCG GCGTTCAACA CATCCACGGG CATTCCGCAT 
GCGCGGGTTA ATCTCCGCCT GGGCATGAAG GACCCGATGC TCAAGAAGTC CCGTGAAACG 
TGTACGGCGT GTGCTGGTAC CATCCTTCTG GAATTTGCTG CTCTGTCGCG TCTCACCGGC 
GATCCCATTT TCGAGGTGCG AGCTCACGCC GCCATGGATG CACTGTGGAA GCTAAGGCAC 
CGTGGCTCCG ATCTCATGGG CACGGTACTT AATGTCCATT CGGGCGATTG GGTGCGTCGC 
GATTCTGGAG TCGGAGCTGG CATCGACAGT TACTATGAGT ACCTGTTCAA GTCGTACGTC 
CTACTGGGTG ACGACAAGTA CCTGGCACGC TTTAATCGCC ACTACAACGC AGTGATGAAG 
TACGTCAGCG AAGGACCCAT GCTACTCGAC GTGCTCATGC ACAGGCCACA TGCTAAGTCC 
AAAAACTTCA TGGACTCGCT TCTGGCATTT TGGCCGGGCT TGCAGGTGCT ATCGGGCGAT 
CTGAAACCAG CTGTGCAGAC ACACGAGATG CTCTATCAGG TCATGCAGAT GCACACCTTC 
ATTCCAGAAG CATTTACCGT CGACTTCCAG ATCCATTGGG GACAGCATCC CCTGCGACCA 
GAATTTATAG AGTCCACTTA CTTCCTGTAC CGCGCCACTG GCGATCATCA TTATCTGCAG 
GTTGGCAAGA AGGCTCTGAA AACACTCCAG CAGCATGCTA AGGTATCTTG CGGCTATGCG 
GCCGTCAATG ATGTACGGAC TGGCAAGCAT GAGGATCGCA TGGACTCGTT TGTGCTCTCC 
GAGACGATCA AGTACCTCTT CCTACTGTTC TCCGATCCCC AGGATCTGAT CATAAACGTC 
GATGAGTTCG TCTTCACGAC CGAGGCTCAT CTGCTACCGC TCTCTATTGC CCAACTAGGG 
AATGCCACGT TTAGCTTTCG CCAGACGGAC GAACACAACG TACTCGACTT CATGCGCACC 
TGCCCCAGTT CAAATAGGCT TTTTCCTGAA AAAGTACGCA AACCACTGCG CAACTTCATT 
ACGGGCTCGT GCCCTCGCAC CACAGCGGGA AAGCGACTCA GCGCCCTGGA TTTTCAGGCA 
AGCAATGCAG ATCATCTTCG AGCTGTTTAC GACATGGGCA TTACCATGGT TTCGGTAGGC 
GACCGCAGCC AAGGCAAGGT GCGACTGTTT CATAGTTTCT ATAATGCCAA AAGTCACGAG 
GAGGGCGAAA TGGGACTGCA ATTTATGCAG GAGATGCTTG AGCTGACCAA GATGCAAAGC 
ATAAATCAGC TAGCGCAACT GCAGGCCGTT GCCTACGCCA CTGACGAGAA CTCACAGGAT 
TGGATCGCAC TGATGGCCGG GCCGTCGCAC TTTAGCCCAG AGTTAACTGG CGATCAGTTC 
GTCGAAGGGG ACGTGATTCT GGCCAAGCCG CTACGAGCAT GCGATGAGAG CCTAGAGAAC 
GCCGAGGAGG CGAAAGGAAA GGTCCTGGTG GCCGAGCGCG GCGATTGCAC GTTCGTTAGC 
AAAGCGCGAC TGGCCCAGAA GGTGGGGGCG GCGGCGCTGA TTGTCTGTGA TAATGTTCCG 
GGCTCTTCCG GTGAGACGCA GCCGATGTTC GCAATGTCTG GCGACGGCAA GGACGACGTG 
CTCATTCCAG TCGTATTCAT GTACAGCATG GAGTTCGGCA AACTGTCGGC GGTCATGCAG 
CGACGGAAAC AACCTCTGCG TGTTCGCGTC ATGCAGATGG TGGAGTTTAA GCGGTGGCAA 
CTGGCCAAGG AGCAGCGCCA GAACCAAACA GCGTCGGTAG CACAGAAACC GGAAAAAGAA 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem2 ( NM_001273505.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260434.1 Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 2 (Edem2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16709
Clone ID Related Accession (Same CDS sequence)	NM_001273505.1
Accession Version	NM_001273505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ER degradation enhancer, mannosidase alpha-like 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTACCA CATCCTCCGA ATCCTTTGTC CGCGGCCAGC GGCTTATGCT CATCTTCCTG 
GTGGCCATGT GCATCGTAGT TGCCTGCGTC CTTAGCACCG AAACCACCAT GCCCACGCAG 
AACATGTCCA ATAAGGAGCG GGCGGAACTC CGGGAAGAGG CTCGCGATAT GTTCTATCAT 
GCATACAACG CCTACATGCA GAACGCCTAT CCAGCTGATG AGCTTATGCC GCTCTCCTGC 
AAAGGACGGT ACCGAGGAGT GACGCCATCA CGTGGCGACA TGGACGACAT CCTTGGAAAT 
TTCTCCATGA CTCTGGTGGA TACTCTGGAC ACTCTGGTGC TCCTGGGCGA TTTCACAGAG 
TTTGATCACG CGGTGAAGCT AGTTATCCGC GACGTTCAAT TCGACAGCGA CATTATTGTG 
TCGGTGTTTG AGACGAACAT TCGAATGGTC GGTGGCTTGC TGTCGGCGCA CATCCTGGCG 
GAGTATCTGC AAAAGCATGC GGACACGATG CATTGGTACA AGGGCGAACT GCTGGAGATG 
TCTCGCGAGC TGGGCTACCG TTTGCTACCG GCGTTCAACA CATCCACGGG CATTCCGCAT 
GCGCGGGTTA ATCTCCGCCT GGGCATGAAG GACCCGATGC TCAAGAAGTC CCGTGAAACG 
TGTACGGCGT GTGCTGGTAC CATCCTTCTG GAATTTGCTG CTCTGTCGCG TCTCACCGGC 
GATCCCATTT TCGAGGTGCG AGCTCACGCC GCCATGGATG CACTGTGGAA GCTAAGGCAC 
CGTGGCTCCG ATCTCATGGG CACGGTACTT AATGTCCATT CGGGCGATTG GGTGCGTCGC 
GATTCTGGAG TCGGAGCTGG CATCGACAGT TACTATGAGT ACCTGTTCAA GTCGTACGTC 
CTACTGGGTG ACGACAAGTA CCTGGCACGC TTTAATCGCC ACTACAACGC AGTGATGAAG 
TACGTCAGCG AAGGACCCAT GCTACTCGAC GTGCTCATGC ACAGGCCACA TGCTAAGTCC 
AAAAACTTCA TGGACTCGCT TCTGGCATTT TGGCCGGGCT TGCAGGTGCT ATCGGGCGAT 
CTGAAACCAG CTGTGCAGAC ACACGAGATG CTCTATCAGG TCATGCAGAT GCACACCTTC 
ATTCCAGAAG CATTTACCGT CGACTTCCAG ATCCATTGGG GACAGCATCC CCTGCGACCA 
GAATTTATAG AGTCCACTTA CTTCCTGTAC CGCGCCACTG GCGATCATCA TTATCTGCAG 
GTTGGCAAGA AGGCTCTGAA AACACTCCAG CAGCATGCTA AGGTATCTTG CGGCTATGCG 
GCCGTCAATG ATGTACGGAC TGGCAAGCAT GAGGATCGCA TGGACTCGTT TGTGCTCTCC 
GAGACGATCA AGTACCTCTT CCTACTGTTC TCCGATCCCC AGGATCTGAT CATAAACGTC 
GATGAGTTCG TCTTCACGAC CGAGGCTCAT CTGCTACCGC TCTCTATTGC CCAACTAGGG 
AATGCCACGT TTAGCTTTCG CCAGACGGAC GAACACAACG TACTCGACTT CATGCGCACC 
TGCCCCAGTT CAAATAGGCT TTTTCCTGAA AAAGTACGCA AACCACTGCG CAACTTCATT 
ACGGGCTCGT GCCCTCGCAC CACAGCGGGA AAGCGACTCA GCGCCCTGGA TTTTCAGGCA 
AGCAATGCAG ATCATCTTCG AGCTGTTTAC GACATGGGCA TTACCATGGT TTCGGTAGGC 
GACCGCAGCC AAGGCAAGGC CAAAAGTCAC GAGGAGGGCG AAATGGGACT GCAATTTATG 
CAGGAGATGC TTGAGCTGAC CAAGATGCAA AGCATAAATC AGCTAGCGCA ACTGCAGGCC 
GTTGCCTACG CCACTGACGA GAACTCACAG GATTGGATCG CACTGATGGC CGGGCCGTCG 
CACTTTAGCC CAGAGTTAAC TGGCGATCAG TTCGTCGAAG GGGACGTGAT TCTGGCCAAG 
CCGCTACGAG CATGCGATGA GAGCCTAGAG AACGCCGAGG AGGCGAAAGG AAAGGTCCTG 
GTGGCCGAGC GCGGCGATTG CACGTTCGTT AGCAAAGCGC GACTGGCCCA GAAGGTGGGG 
GCGGCGGCGC TGATTGTCTG TGATAATGTT CCGGGCTCTT CCGGTGAGAC GCAGCCGATG 
TTCGCAATGT CTGGCGACGG CAAGGACGAC GTGCTCATTC CAGTCGTATT CATGTACAGC 
ATGGAGTTCG GCAAACTGTC GGCGGTCATG CAGCGACGGA AACAACCTCT GCGTGTTCGC 
GTCATGCAGA TGGTGGAGTT TAAGCGGTGG CAACTGGCCA AGGAGCAGCG CCAGAACCAA 
ACAGCGTCGG TAGCACAGAA ACCGGAAAAA GAATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260434.1
CDS	670..3048
Translation	MSTTSSESFVRGQRLMLIFLVAMCIVVACVLSTETTMPTQNMSNKERAELREEARDMFYHAYNAYMQNAYPADELMPLSCKGRYRGVTPSRGDMDDILGNFSMTLVDTLDTLVLLGDFTEFDHAVKLVIRDVQFDSDIIVSVFETNIRMVGGLLSAHILAEYLQKHADTMHWYKGELLEMSRELGYRLLPAFNTSTGIPHARVNLRLGMKDPMLKKSRETCTACAGTILLEFAALSRLTGDPIFEVRAHAAMDALWKLRHRGSDLMGTVLNVHSGDWVRRDSGVGAGIDSYYEYLFKSYVLLGDDKYLARFNRHYNAVMKYVSEGPMLLDVLMHRPHAKSKNFMDSLLAFWPGLQVLSGDLKPAVQTHEMLYQVMQMHTFIPEAFTVDFQIHWGQHPLRPEFIESTYFLYRATGDHHYLQVGKKALKTLQQHAKVSCGYAAVNDVRTGKHEDRMDSFVLSETIKYLFLLFSDPQDLIINVDEFVFTTEAHLLPLSIAQLGNATFSFRQTDEHNVLDFMRTCPSSNRLFPEKVRKPLRNFITGSCPRTTAGKRLSALDFQASNADHLRAVYDMGITMVSVGDRSQGKAKSHEEGEMGLQFMQEMLELTKMQSINQLAQLQAVAYATDENSQDWIALMAGPSHFSPELTGDQFVEGDVILAKPLRACDESLENAEEAKGKVLVAERGDCTFVSKARLAQKVGAAALIVCDNVPGSSGETQPMFAMSGDGKDDVLIPVVFMYSMEFGKLSAVMQRRKQPLRVRVMQMVEFKRWQLAKEQRQNQTASVAQKPEKEL

Target ORF information:

RefSeq Version	NM_001273505.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 2 (Edem2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273505.1

ORF Insert Sequence:

ATGTCTACCA CATCCTCCGA ATCCTTTGTC CGCGGCCAGC GGCTTATGCT CATCTTCCTG 
GTGGCCATGT GCATCGTAGT TGCCTGCGTC CTTAGCACCG AAACCACCAT GCCCACGCAG 
AACATGTCCA ATAAGGAGCG GGCGGAACTC CGGGAAGAGG CTCGCGATAT GTTCTATCAT 
GCATACAACG CCTACATGCA GAACGCCTAT CCAGCTGATG AGCTTATGCC GCTCTCCTGC 
AAAGGACGGT ACCGAGGAGT GACGCCATCA CGTGGCGACA TGGACGACAT CCTTGGAAAT 
TTCTCCATGA CTCTGGTGGA TACTCTGGAC ACTCTGGTGC TCCTGGGCGA TTTCACAGAG 
TTTGATCACG CGGTGAAGCT AGTTATCCGC GACGTTCAAT TCGACAGCGA CATTATTGTG 
TCGGTGTTTG AGACGAACAT TCGAATGGTC GGTGGCTTGC TGTCGGCGCA CATCCTGGCG 
GAGTATCTGC AAAAGCATGC GGACACGATG CATTGGTACA AGGGCGAACT GCTGGAGATG 
TCTCGCGAGC TGGGCTACCG TTTGCTACCG GCGTTCAACA CATCCACGGG CATTCCGCAT 
GCGCGGGTTA ATCTCCGCCT GGGCATGAAG GACCCGATGC TCAAGAAGTC CCGTGAAACG 
TGTACGGCGT GTGCTGGTAC CATCCTTCTG GAATTTGCTG CTCTGTCGCG TCTCACCGGC 
GATCCCATTT TCGAGGTGCG AGCTCACGCC GCCATGGATG CACTGTGGAA GCTAAGGCAC 
CGTGGCTCCG ATCTCATGGG CACGGTACTT AATGTCCATT CGGGCGATTG GGTGCGTCGC 
GATTCTGGAG TCGGAGCTGG CATCGACAGT TACTATGAGT ACCTGTTCAA GTCGTACGTC 
CTACTGGGTG ACGACAAGTA CCTGGCACGC TTTAATCGCC ACTACAACGC AGTGATGAAG 
TACGTCAGCG AAGGACCCAT GCTACTCGAC GTGCTCATGC ACAGGCCACA TGCTAAGTCC 
AAAAACTTCA TGGACTCGCT TCTGGCATTT TGGCCGGGCT TGCAGGTGCT ATCGGGCGAT 
CTGAAACCAG CTGTGCAGAC ACACGAGATG CTCTATCAGG TCATGCAGAT GCACACCTTC 
ATTCCAGAAG CATTTACCGT CGACTTCCAG ATCCATTGGG GACAGCATCC CCTGCGACCA 
GAATTTATAG AGTCCACTTA CTTCCTGTAC CGCGCCACTG GCGATCATCA TTATCTGCAG 
GTTGGCAAGA AGGCTCTGAA AACACTCCAG CAGCATGCTA AGGTATCTTG CGGCTATGCG 
GCCGTCAATG ATGTACGGAC TGGCAAGCAT GAGGATCGCA TGGACTCGTT TGTGCTCTCC 
GAGACGATCA AGTACCTCTT CCTACTGTTC TCCGATCCCC AGGATCTGAT CATAAACGTC 
GATGAGTTCG TCTTCACGAC CGAGGCTCAT CTGCTACCGC TCTCTATTGC CCAACTAGGG 
AATGCCACGT TTAGCTTTCG CCAGACGGAC GAACACAACG TACTCGACTT CATGCGCACC 
TGCCCCAGTT CAAATAGGCT TTTTCCTGAA AAAGTACGCA AACCACTGCG CAACTTCATT 
ACGGGCTCGT GCCCTCGCAC CACAGCGGGA AAGCGACTCA GCGCCCTGGA TTTTCAGGCA 
AGCAATGCAG ATCATCTTCG AGCTGTTTAC GACATGGGCA TTACCATGGT TTCGGTAGGC 
GACCGCAGCC AAGGCAAGGC CAAAAGTCAC GAGGAGGGCG AAATGGGACT GCAATTTATG 
CAGGAGATGC TTGAGCTGAC CAAGATGCAA AGCATAAATC AGCTAGCGCA ACTGCAGGCC 
GTTGCCTACG CCACTGACGA GAACTCACAG GATTGGATCG CACTGATGGC CGGGCCGTCG 
CACTTTAGCC CAGAGTTAAC TGGCGATCAG TTCGTCGAAG GGGACGTGAT TCTGGCCAAG 
CCGCTACGAG CATGCGATGA GAGCCTAGAG AACGCCGAGG AGGCGAAAGG AAAGGTCCTG 
GTGGCCGAGC GCGGCGATTG CACGTTCGTT AGCAAAGCGC GACTGGCCCA GAAGGTGGGG 
GCGGCGGCGC TGATTGTCTG TGATAATGTT CCGGGCTCTT CCGGTGAGAC GCAGCCGATG 
TTCGCAATGT CTGGCGACGG CAAGGACGAC GTGCTCATTC CAGTCGTATT CATGTACAGC 
ATGGAGTTCG GCAAACTGTC GGCGGTCATG CAGCGACGGA AACAACCTCT GCGTGTTCGC 
GTCATGCAGA TGGTGGAGTT TAAGCGGTGG CAACTGGCCA AGGAGCAGCG CCAGAACCAA 
ACAGCGTCGG TAGCACAGAA ACCGGAAAAA GAATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Edem2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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