Vha68-3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vha68-3
Entrez Gene ID	34695
Full Name	Vacuolar H[+] ATPase 68kD subunit 3
Synonyms	CG5075,Dmel\CG5075,Vha68,vha67-3,vha68-3
General protein information	Preferred Names Vacuolar H[+] ATPase 68kD subunit 3 Names CG5075 gene product from transcript CG5075-RA CG5075-PA Vha68-3-PA vacuolar H[+] ATPase 68kD subunit 3 vacuolar H[+] ATPase subunit 68-3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34A3-34A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_135751.3	NP_609595.1	vacuolar H[+] ATPase 68kD subunit 3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vha68-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vha68-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14588	NM_135751.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 68kD subunit 3 (Vha68-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vha68-3 ( NM_135751.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609595.1 Drosophila melanogaster vacuolar H[+] ATPase 68kD subunit 3 (Vha68-3), mRNA.

Price & Availability↑

CloneID	OFa14588
Clone ID Related Accession (Same CDS sequence)	NM_135751.3
Accession Version	NM_135751.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 68kD subunit 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135751.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGA AGTCCTGGGT TGCACCGCTT ACCTCGTTCA CCAACAGTAA CGCAAGTAAC 
GAAGACAGAG CCCGCAACTC GGGATCGTTA AACCAAGATG GGGAGCCAAG GTCCGTGCCT 
CACCCAACGG TTCCGGCTAA GAAGTGCACA TGTCCAACCT GCGCTTGCCA ACGTAATGAC 
CATAAATCCA ATCCGGTACC CCCTCCCCCT CAATCACGCA AGCCTGTAGC CGAGCCGGAA 
AGTCCCCATG ATACAGTCAA TGACGAGGAC AGTTTGAAGG ACTTGAGACG TTCGACGGAC 
CAATCCCACA AGAGCGCTCA CATCGCCTTG GAGAAGAATG AGGACTCGGG TTTTGTGATC 
GAGCAGGTGG TTGATACGCA CAAATATTCG TCGGATGAAG AAGAGGAGGA GGCGACGATG 
GGTCGCATTT TCGGAGTCTC CGGTCCGGTG GTCAATGCCG AGGAGATGGC CGGCGCAGCC 
ATGTACGAGC TGGTTCGCGT TGGACACTCC CAGCTTGTTG GTGAGATCAT TCGACTGGAG 
GGTGATATGG CCACCATTCA GGTTTACGAG GATACTTCGG GTGTGAGCGT GGGTGATCCC 
GTCTACCAGA CGGGAAAGCC GCTCTCCGTG GAATTGGGAC CCGGCATCAT GGGCAGCATT 
TTCGATGGTA TCCAGCGACC ATTGAGGTCC ATCAGTGAAC TAACCAACTC CATATACGTG 
CCCAAGGGCA TCGATACGCC CTCCCTGCCC AGGAACATTG CGTACGAATT CACACCCGGA 
AAATTGAAGA TCGATGCTCT GATCACCGGC GGAGACATCT ACGGATCTGT TTTCGAAAAC 
AGCATGATGC ACGATCACCG CCTGATACTA CCGCCCCGCA CCAAGGGGCG CATCCGGTGG 
TTGGCCCCGC CCGGGAACTA CTGCGTGGAC GAGGTGATCG TGGAGACGGA GTTCAACGAC 
GAGATCACCA AGCACACCAT GCTCCAGGTG TGGCCCGTAC GGAGGTGTCG TCCGGTGGAG 
GATAAGCTCC CCAGCAATTC ACCACTCTTG ACTGGCCAGC GCGTCCTGGA CGCCTTCTTT 
CCATGTGTCC AGGGCGGAAC CACTGCCATT CCAGGAGCGT TTGGATGTGG AAAGACCGTC 
ATCTCACAGG CCCTGTCCAA ATACTCCAAC TCAGATGTCA TCATCTACGT GGGCTGCGGT 
GAGCGCGGGA ACGAAATGTC CGAGGTTCTT CGGGACTTTC CGCAGCTTGA GGTTGAGGTC 
AATGGCACCA TGGAATCGAT TATGAAGCGG ACTGCCCTGG TGGCCAATAC CTCCAATATG 
CCGGTGGCTG CCCGAGAAGC CTCGATCTAC ACGGGCATCA CCCTCTCGGA ATACTTCCGC 
GACATGGGCT ACCATGTGTC CATGATGGCG GACTCCACAT CTCGATGGGC GGAGGCGCTG 
CGTGAGATTT CCGGACGTCT GGCCGAGATG CCTGCCGATG CTGGCTATCC GGCTTACTTG 
GGAGCTCGGC TGGCCTCCTT CTACGAGAGA GCCGGACTGG TGAAGTGCCT GGGCAGTCCA 
GATCGTGAGG GATCCGTGTC CATTGTGGGA GCTGTGTCGC CTCCCGGTGG CGACTTCTCT 
GATCCCGTGA CCTCCGCCAC CCTGAGCATC GTTCAGGTCT TTTGGGGTCT GGACAAGAAG 
CTGGCCCAGA GGAAACACTT TCCATCGGTG AACTGGCTGC AATCGTACTC CAAGTACATG 
CGCACTTTGG ACACTTACTA CGAGGAATCC AATCCGGAGT TTACGCATCT GCGAGCTAAG 
GCCAAGAAGG TGCTGCAGGA GGAGGATGAC CTGGCGGAAA TTGTCCAACT GGTGGGCAAG 
TCGTCGTTGA ACGAGGAGGA CAAGATCACC CTTGAGGTGG CCAAGATGCT GAAGGACGAT 
TTCCTGCAGC AGAACTCCTA CAGTCAGTAC GATGCCTACT GCCCCTTCTT CAAGACCATT 
GGCATGCTGA AGAACATGAT GACCTTCTAT GATGCGGCGA TCCTGTCGGT GCAGAACACG 
GCGGACAATG AGGCGCGTGT AACTTGGGGC TTGATCCGTA TAAAAGCCGC TAACATTCTG 
TACGAGCTCT CCACCATGAA GTTCATTGAT CCCAAGCTGG GCGAAGCGAC TGTCCTGGAG 
AGCATGAACA AGCTGCATGA CAATATCCTT TCAACTTTCC GGGAGATAGA GGATAATGTC 
GAGGACTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609595.1
CDS	115..2346
Translation	MEKKSWVAPLTSFTNSNASNEDRARNSGSLNQDGEPRSVPHPTVPAKKCTCPTCACQRNDHKSNPVPPPPQSRKPVAEPESPHDTVNDEDSLKDLRRSTDQSHKSAHIALEKNEDSGFVIEQVVDTHKYSSDEEEEEATMGRIFGVSGPVVNAEEMAGAAMYELVRVGHSQLVGEIIRLEGDMATIQVYEDTSGVSVGDPVYQTGKPLSVELGPGIMGSIFDGIQRPLRSISELTNSIYVPKGIDTPSLPRNIAYEFTPGKLKIDALITGGDIYGSVFENSMMHDHRLILPPRTKGRIRWLAPPGNYCVDEVIVETEFNDEITKHTMLQVWPVRRCRPVEDKLPSNSPLLTGQRVLDAFFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPQLEVEVNGTMESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADAGYPAYLGARLASFYERAGLVKCLGSPDREGSVSIVGAVSPPGGDFSDPVTSATLSIVQVFWGLDKKLAQRKHFPSVNWLQSYSKYMRTLDTYYEESNPEFTHLRAKAKKVLQEEDDLAEIVQLVGKSSLNEEDKITLEVAKMLKDDFLQQNSYSQYDAYCPFFKTIGMLKNMMTFYDAAILSVQNTADNEARVTWGLIRIKAANILYELSTMKFIDPKLGEATVLESMNKLHDNILSTFREIEDNVEDY

Target ORF information:

RefSeq Version	NM_135751.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 68kD subunit 3 (Vha68-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135751.3

ORF Insert Sequence:

ATGGAAAAGA AGTCCTGGGT TGCACCGCTT ACCTCGTTCA CCAACAGTAA CGCAAGTAAC 
GAAGACAGAG CCCGCAACTC GGGATCGTTA AACCAAGATG GGGAGCCAAG GTCCGTGCCT 
CACCCAACGG TTCCGGCTAA GAAGTGCACA TGTCCAACCT GCGCTTGCCA ACGTAATGAC 
CATAAATCCA ATCCGGTACC CCCTCCCCCT CAATCACGCA AGCCTGTAGC CGAGCCGGAA 
AGTCCCCATG ATACAGTCAA TGACGAGGAC AGTTTGAAGG ACTTGAGACG TTCGACGGAC 
CAATCCCACA AGAGCGCTCA CATCGCCTTG GAGAAGAATG AGGACTCGGG TTTTGTGATC 
GAGCAGGTGG TTGATACGCA CAAATATTCG TCGGATGAAG AAGAGGAGGA GGCGACGATG 
GGTCGCATTT TCGGAGTCTC CGGTCCGGTG GTCAATGCCG AGGAGATGGC CGGCGCAGCC 
ATGTACGAGC TGGTTCGCGT TGGACACTCC CAGCTTGTTG GTGAGATCAT TCGACTGGAG 
GGTGATATGG CCACCATTCA GGTTTACGAG GATACTTCGG GTGTGAGCGT GGGTGATCCC 
GTCTACCAGA CGGGAAAGCC GCTCTCCGTG GAATTGGGAC CCGGCATCAT GGGCAGCATT 
TTCGATGGTA TCCAGCGACC ATTGAGGTCC ATCAGTGAAC TAACCAACTC CATATACGTG 
CCCAAGGGCA TCGATACGCC CTCCCTGCCC AGGAACATTG CGTACGAATT CACACCCGGA 
AAATTGAAGA TCGATGCTCT GATCACCGGC GGAGACATCT ACGGATCTGT TTTCGAAAAC 
AGCATGATGC ACGATCACCG CCTGATACTA CCGCCCCGCA CCAAGGGGCG CATCCGGTGG 
TTGGCCCCGC CCGGGAACTA CTGCGTGGAC GAGGTGATCG TGGAGACGGA GTTCAACGAC 
GAGATCACCA AGCACACCAT GCTCCAGGTG TGGCCCGTAC GGAGGTGTCG TCCGGTGGAG 
GATAAGCTCC CCAGCAATTC ACCACTCTTG ACTGGCCAGC GCGTCCTGGA CGCCTTCTTT 
CCATGTGTCC AGGGCGGAAC CACTGCCATT CCAGGAGCGT TTGGATGTGG AAAGACCGTC 
ATCTCACAGG CCCTGTCCAA ATACTCCAAC TCAGATGTCA TCATCTACGT GGGCTGCGGT 
GAGCGCGGGA ACGAAATGTC CGAGGTTCTT CGGGACTTTC CGCAGCTTGA GGTTGAGGTC 
AATGGCACCA TGGAATCGAT TATGAAGCGG ACTGCCCTGG TGGCCAATAC CTCCAATATG 
CCGGTGGCTG CCCGAGAAGC CTCGATCTAC ACGGGCATCA CCCTCTCGGA ATACTTCCGC 
GACATGGGCT ACCATGTGTC CATGATGGCG GACTCCACAT CTCGATGGGC GGAGGCGCTG 
CGTGAGATTT CCGGACGTCT GGCCGAGATG CCTGCCGATG CTGGCTATCC GGCTTACTTG 
GGAGCTCGGC TGGCCTCCTT CTACGAGAGA GCCGGACTGG TGAAGTGCCT GGGCAGTCCA 
GATCGTGAGG GATCCGTGTC CATTGTGGGA GCTGTGTCGC CTCCCGGTGG CGACTTCTCT 
GATCCCGTGA CCTCCGCCAC CCTGAGCATC GTTCAGGTCT TTTGGGGTCT GGACAAGAAG 
CTGGCCCAGA GGAAACACTT TCCATCGGTG AACTGGCTGC AATCGTACTC CAAGTACATG 
CGCACTTTGG ACACTTACTA CGAGGAATCC AATCCGGAGT TTACGCATCT GCGAGCTAAG 
GCCAAGAAGG TGCTGCAGGA GGAGGATGAC CTGGCGGAAA TTGTCCAACT GGTGGGCAAG 
TCGTCGTTGA ACGAGGAGGA CAAGATCACC CTTGAGGTGG CCAAGATGCT GAAGGACGAT 
TTCCTGCAGC AGAACTCCTA CAGTCAGTAC GATGCCTACT GCCCCTTCTT CAAGACCATT 
GGCATGCTGA AGAACATGAT GACCTTCTAT GATGCGGCGA TCCTGTCGGT GCAGAACACG 
GCGGACAATG AGGCGCGTGT AACTTGGGGC TTGATCCGTA TAAAAGCCGC TAACATTCTG 
TACGAGCTCT CCACCATGAA GTTCATTGAT CCCAAGCTGG GCGAAGCGAC TGTCCTGGAG 
AGCATGAACA AGCTGCATGA CAATATCCTT TCAACTTTCC GGGAGATAGA GGATAATGTC 
GAGGACTATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vha68-3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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