bru1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following bru1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bru1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa14526 NM_165005.3
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa14523 NM_165002.2
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa14523 NM_001273474.1
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa14524 NM_165003.3
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa14525 NM_001169492.2
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa14525 NM_165004.3
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa16707 NM_001273478.1
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa16707 NM_001273477.1
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa16707 NM_001273476.1
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa16707 NM_001273475.1
Latest version!
Drosophila melanogaster bruno 1 (bru1), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa14526
    Clone ID Related Accession (Same CDS sequence) NM_165005.3
    Accession Version NM_165005.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165005.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTAAACA AGAAGCTGAA CGAGAACGAC GTTCGCAAGC TATTTGAAGT CCACGGAGCC 
    ATCGAGGAGT GCACCGTCCT TCGGGACCAA AACGGACAGA GCAAGGGTTG TGCCTTCGTC
    ACATTCGCCA CCAAGCACGC TGCCATTTCG GCCATTAAAG TGACCCTGAG CCAGAACAAG
    ATCATGGAGG GCTGCACATC GCCGCTGGTC GTCAAGTTCG CCGATACGCA AAAAGAGAAG
    GAGCAGAAGA AGATTCAGCA AATTCAGGCC AATCTCTGGA ACCTGGCCAG CAACATCAAC
    ATACCTCTGG GCCAAACGGC CACCAGCGTG ACCACGCCCA TCCTGCCCAA TCCGCCGCAG
    CAGCCTTCGC CGGTTCTCGG CGCAGATGCC ATTACCCCGG CCTCCATTCA ACTGCTGCAG
    CAGCTGCAGG CGGTGGGACT GCAGCACCAG TTGTTGCAAG CCCTCACAGG ACTGGGAGCC
    CAGCAGAGCA GCTCGGCGAC GGACACCAGC GCTGTGGCGG CCGCAGGACT TTTGACCCCG
    ATGACCGTCC AGAATCTAGC GGCCATTGCG GCCATGACGC AGCCGAGTCT CTCGAATGCC
    GCCGCGGCAG CCGCCGCTGC CACATCGCCT GGAAGCGCCC AACTGACCAA CACGGCTGCG
    CTGCTCTGGT CCGATCCGAA TCCAATGGCA TCGGCATATA TGTCAACAGC CGCCGGTCTG
    CCGCAATTCG GGAGCGCCAG TGCATTGTCC ACTTCGCCGC TGGCCAGCGT TGCCCTCAGC
    GCCGCAGCCG CCGCCGCAGC GGGAAAGCAG ATTGAAGGAC CCGAGGGCTG CAACCTTTTC
    ATCTACCATC TGCCGCAGGA GTTCACCGAT ACGGATCTGG CCTCCACGTT CCTGCCCTTC
    GGCAATGTGA TCTCGGCCAA GGTGTTCATC GACAAACAGA CGAGCCTGTC GAAGTGCTTC
    GGGTTTGTCT CCTTCGACAA TCCGGACTCG GCCCAGGTGG CCATAAAGGC GATGAACGGC
    TTCCAGGTGG GCACCAAGCG TCTCAAGGTG CAGCTGAAGA AGCCCAAGGA CTCGAAGCCC
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723740.1
    CDS1047..2132
    Translation

    Target ORF information:

    RefSeq Version NM_165005.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165005.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCTAAACA AGAAGCTGAA CGAGAACGAC GTTCGCAAGC TATTTGAAGT CCACGGAGCC 
    ATCGAGGAGT GCACCGTCCT TCGGGACCAA AACGGACAGA GCAAGGGTTG TGCCTTCGTC
    ACATTCGCCA CCAAGCACGC TGCCATTTCG GCCATTAAAG TGACCCTGAG CCAGAACAAG
    ATCATGGAGG GCTGCACATC GCCGCTGGTC GTCAAGTTCG CCGATACGCA AAAAGAGAAG
    GAGCAGAAGA AGATTCAGCA AATTCAGGCC AATCTCTGGA ACCTGGCCAG CAACATCAAC
    ATACCTCTGG GCCAAACGGC CACCAGCGTG ACCACGCCCA TCCTGCCCAA TCCGCCGCAG
    CAGCCTTCGC CGGTTCTCGG CGCAGATGCC ATTACCCCGG CCTCCATTCA ACTGCTGCAG
    CAGCTGCAGG CGGTGGGACT GCAGCACCAG TTGTTGCAAG CCCTCACAGG ACTGGGAGCC
    CAGCAGAGCA GCTCGGCGAC GGACACCAGC GCTGTGGCGG CCGCAGGACT TTTGACCCCG
    ATGACCGTCC AGAATCTAGC GGCCATTGCG GCCATGACGC AGCCGAGTCT CTCGAATGCC
    GCCGCGGCAG CCGCCGCTGC CACATCGCCT GGAAGCGCCC AACTGACCAA CACGGCTGCG
    CTGCTCTGGT CCGATCCGAA TCCAATGGCA TCGGCATATA TGTCAACAGC CGCCGGTCTG
    CCGCAATTCG GGAGCGCCAG TGCATTGTCC ACTTCGCCGC TGGCCAGCGT TGCCCTCAGC
    GCCGCAGCCG CCGCCGCAGC GGGAAAGCAG ATTGAAGGAC CCGAGGGCTG CAACCTTTTC
    ATCTACCATC TGCCGCAGGA GTTCACCGAT ACGGATCTGG CCTCCACGTT CCTGCCCTTC
    GGCAATGTGA TCTCGGCCAA GGTGTTCATC GACAAACAGA CGAGCCTGTC GAAGTGCTTC
    GGGTTTGTCT CCTTCGACAA TCCGGACTCG GCCCAGGTGG CCATAAAGGC GATGAACGGC
    TTCCAGGTGG GCACCAAGCG TCTCAAGGTG CAGCTGAAGA AGCCCAAGGA CTCGAAGCCC
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14523
    Clone ID Related Accession (Same CDS sequence) NM_165002.2 , NM_001273474.1
    Accession Version NM_165002.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_165002.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTCAGTT CGCTGGACGC GCTGGCCGGC AAAATAGCCA CTGCCACGCC CGGAACGGGG 
    GCGGTAACGG CGGACAGCAA GAACACACAG TCGCTGCACC ATCACCATCG CCTGGACTAT
    GATTACTCGG CGGTTGAGAT TGAACCACCG GCGGAACAAC TGGCCAATTC ACCGCGCAGC
    GAACGGGAGC GACTGCAGCA GGCCCAACAG GCCTACGAGC AGCAGGCCCA GGCGGAACTG
    GAGTTCGGTC TCGCGGAGGT GGATGCCAAG CTGACGCAGC TAAAGAGCAG CCACAGTCTG
    CAGCATCACC ACAGTCATCA CCACCAGCTG CAGCGCCATA CGGCGCTCAA TCAGCAACAG
    CAGCAGCTGC ACCACCATCA GCCCCACCAC AATCATCACC ACCATCATCC GTACCAGCGA
    CCCAGACCCA CATCGCCACC GCAACAACAG ACGTCCTCAT ACCAGCAGCA ACTTTCCCAG
    CTGGCCGCTC TGCAGGATCA CCATGATCTC CTCCAGACGC AGCAGGAGCT CTTCCACAAG
    CAGCTGGCCA CCATGCAGGA GTACCAGCGG GAAAGGGAAC GGGAACGTGA GCGGGAAAGG
    GAGCGGGAAC GTGAGCGGGA ACGGGAGCGG GATCGCAGCA GCTTCATTGA TAACAGCGAT
    TACGATTCCC AGACGGAGTT CAAAATTTGC CTGAGGGATA GTGCTTTTGG TATGAGTGAT
    AAGAGCAGCA GTGCAACGAA CAGCCTGCCG AATAGTCCCA TACACAGCAA CAACAATAAT
    CCATCGCTGC TGAACAACAA CAACAATAAC AGCAACGGCA CCAGCAGCAA CAACAGCTTA
    AACGTTGGCA ACAACAACAG CAATCCGAGC TTGGGCGGCA GCAACAGCAA CGCACTGGTT
    TCCGTAGGCA GCAACGGTAT AATGTCCGCC GCCGGTTTGG TCAACAACAA TAACAATCCG
    TGCAGCGCCA ACCGCAATGT GGTCGCCATG GTCGACGATG ACGCCTGCTT CCGTCTGGAT
    ACGGACGCCA CGGTGACGTA CGGCGAGAAG GAACCGGATC CGGACAACAT CAAGATGTTC
    GTCGGCCAGG TGCCCAAGTC GATGGACGAG TCGCAGCTGC GCGAGATGTT CGAGGAGTAC
    GGCGCCGTGC ACTCGATCAA CGTTCTGCGG GACAAGGCTA CCGGAATTAG CAAGGGTTGC
    TGCTTTGTGA CATTTTATAC GAGACACGCC GCCCTGAAAG CCCAGGATGC TTTGCATAAT
    GTTAAGACGC TAAATGGGAT GTACCATCCC ATTCAAATGA AGCCCGCCGA TAGCGAGAAT
    CGCAATGAAC GCAAACTCTT TGTGGGTATG CTAAACAAGA AGCTGAACGA GAACGACGTT
    CGCAAGCTAT TTGAAGTCCA CGGAGCCATC GAGGAGTGCA CCGTCCTTCG GGACCAAAAC
    GGACAGAGCA AGGGTTGTGC CTTCGTCACA TTCGCCACCA AGCACGCTGC CATTTCGGCC
    ATTAAAGTGA CCCTGAGCCA GAACAAGATC ATGGAGGGCT GCACATCGCC GCTGGTCGTC
    AAGTTCGCCG ATACGCAAAA AGAGAAGGAG CAGAAGAAGA TTCAGCAAAT TCAGGCCAAT
    CTCTGGAACC TGGCCAGCAA CATCAACATA CCTCTGGGCC AAACGGCCAC CAGCGTGACC
    ACGCCCATCC TGCCCAATCC GCCGCAGCAG CCTTCGCCGG TTCTCGGCGC AGATGCCATT
    ACCCCGGCCT CCATTCAACT GCTGCAGCAG CTGCAGGCGG TGGGACTGCA GCACCAGTTG
    TTGCAAGCCC TCACAGGACT GGGAGCCCAG CAGAGCAGCT CGGCGACGGA CACCAGCGCT
    GTGGCGGCCG CAGGACTTTT GACCCCGATG ACCGTCCAGA ATCTAGCGGC CATTGCGGCC
    ATGACGCAGC CGAGTCTCTC GAATGCCGCC GCGGCAGCCG CCGCTGCCAC ATCGCCTGGA
    AGCGCCCAAC TGACCAACAC GGCTGCGCTG CTCTGGTCCG ATCCGAATCC AATGGCATCG
    GCATATATGT CAACAGCCGC CGGTCTGCCG CAATTCGGGA GCGCCAGTGC ATTGTCCACT
    TCGCCGCTGG CCAGCGTTGC CCTCAGCGCC GCAGCCGCCG CCGCAGCGGG AAAGCAGATT
    GAAGGACCCG AGGGCTGCAA CCTTTTCATC TACCATCTGC CGCAGGAGTT CACCGATACG
    GATCTGGCCT CCACGTTCCT GCCCTTCGGC AATGTGATCT CGGCCAAGGT GTTCATCGAC
    AAACAGACGA GCCTGTCGAA GTGCTTCGGG TTTGTCTCCT TCGACAATCC GGACTCGGCC
    CAGGTGGCCA TAAAGGCGAT GAACGGCTTC CAGGTGGGCA CCAAGCGTCT CAAGGTGCAG
    CTGAAGAAGC CCAAGGACTC GAAGCCCTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723737.1
    CDS501..2933
    Translation

    Target ORF information:

    RefSeq Version NM_165002.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165002.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTCAGTT CGCTGGACGC GCTGGCCGGC AAAATAGCCA CTGCCACGCC CGGAACGGGG 
    GCGGTAACGG CGGACAGCAA GAACACACAG TCGCTGCACC ATCACCATCG CCTGGACTAT
    GATTACTCGG CGGTTGAGAT TGAACCACCG GCGGAACAAC TGGCCAATTC ACCGCGCAGC
    GAACGGGAGC GACTGCAGCA GGCCCAACAG GCCTACGAGC AGCAGGCCCA GGCGGAACTG
    GAGTTCGGTC TCGCGGAGGT GGATGCCAAG CTGACGCAGC TAAAGAGCAG CCACAGTCTG
    CAGCATCACC ACAGTCATCA CCACCAGCTG CAGCGCCATA CGGCGCTCAA TCAGCAACAG
    CAGCAGCTGC ACCACCATCA GCCCCACCAC AATCATCACC ACCATCATCC GTACCAGCGA
    CCCAGACCCA CATCGCCACC GCAACAACAG ACGTCCTCAT ACCAGCAGCA ACTTTCCCAG
    CTGGCCGCTC TGCAGGATCA CCATGATCTC CTCCAGACGC AGCAGGAGCT CTTCCACAAG
    CAGCTGGCCA CCATGCAGGA GTACCAGCGG GAAAGGGAAC GGGAACGTGA GCGGGAAAGG
    GAGCGGGAAC GTGAGCGGGA ACGGGAGCGG GATCGCAGCA GCTTCATTGA TAACAGCGAT
    TACGATTCCC AGACGGAGTT CAAAATTTGC CTGAGGGATA GTGCTTTTGG TATGAGTGAT
    AAGAGCAGCA GTGCAACGAA CAGCCTGCCG AATAGTCCCA TACACAGCAA CAACAATAAT
    CCATCGCTGC TGAACAACAA CAACAATAAC AGCAACGGCA CCAGCAGCAA CAACAGCTTA
    AACGTTGGCA ACAACAACAG CAATCCGAGC TTGGGCGGCA GCAACAGCAA CGCACTGGTT
    TCCGTAGGCA GCAACGGTAT AATGTCCGCC GCCGGTTTGG TCAACAACAA TAACAATCCG
    TGCAGCGCCA ACCGCAATGT GGTCGCCATG GTCGACGATG ACGCCTGCTT CCGTCTGGAT
    ACGGACGCCA CGGTGACGTA CGGCGAGAAG GAACCGGATC CGGACAACAT CAAGATGTTC
    GTCGGCCAGG TGCCCAAGTC GATGGACGAG TCGCAGCTGC GCGAGATGTT CGAGGAGTAC
    GGCGCCGTGC ACTCGATCAA CGTTCTGCGG GACAAGGCTA CCGGAATTAG CAAGGGTTGC
    TGCTTTGTGA CATTTTATAC GAGACACGCC GCCCTGAAAG CCCAGGATGC TTTGCATAAT
    GTTAAGACGC TAAATGGGAT GTACCATCCC ATTCAAATGA AGCCCGCCGA TAGCGAGAAT
    CGCAATGAAC GCAAACTCTT TGTGGGTATG CTAAACAAGA AGCTGAACGA GAACGACGTT
    CGCAAGCTAT TTGAAGTCCA CGGAGCCATC GAGGAGTGCA CCGTCCTTCG GGACCAAAAC
    GGACAGAGCA AGGGTTGTGC CTTCGTCACA TTCGCCACCA AGCACGCTGC CATTTCGGCC
    ATTAAAGTGA CCCTGAGCCA GAACAAGATC ATGGAGGGCT GCACATCGCC GCTGGTCGTC
    AAGTTCGCCG ATACGCAAAA AGAGAAGGAG CAGAAGAAGA TTCAGCAAAT TCAGGCCAAT
    CTCTGGAACC TGGCCAGCAA CATCAACATA CCTCTGGGCC AAACGGCCAC CAGCGTGACC
    ACGCCCATCC TGCCCAATCC GCCGCAGCAG CCTTCGCCGG TTCTCGGCGC AGATGCCATT
    ACCCCGGCCT CCATTCAACT GCTGCAGCAG CTGCAGGCGG TGGGACTGCA GCACCAGTTG
    TTGCAAGCCC TCACAGGACT GGGAGCCCAG CAGAGCAGCT CGGCGACGGA CACCAGCGCT
    GTGGCGGCCG CAGGACTTTT GACCCCGATG ACCGTCCAGA ATCTAGCGGC CATTGCGGCC
    ATGACGCAGC CGAGTCTCTC GAATGCCGCC GCGGCAGCCG CCGCTGCCAC ATCGCCTGGA
    AGCGCCCAAC TGACCAACAC GGCTGCGCTG CTCTGGTCCG ATCCGAATCC AATGGCATCG
    GCATATATGT CAACAGCCGC CGGTCTGCCG CAATTCGGGA GCGCCAGTGC ATTGTCCACT
    TCGCCGCTGG CCAGCGTTGC CCTCAGCGCC GCAGCCGCCG CCGCAGCGGG AAAGCAGATT
    GAAGGACCCG AGGGCTGCAA CCTTTTCATC TACCATCTGC CGCAGGAGTT CACCGATACG
    GATCTGGCCT CCACGTTCCT GCCCTTCGGC AATGTGATCT CGGCCAAGGT GTTCATCGAC
    AAACAGACGA GCCTGTCGAA GTGCTTCGGG TTTGTCTCCT TCGACAATCC GGACTCGGCC
    CAGGTGGCCA TAAAGGCGAT GAACGGCTTC CAGGTGGGCA CCAAGCGTCT CAAGGTGCAG
    CTGAAGAAGC CCAAGGACTC GAAGCCCTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14523
    Clone ID Related Accession (Same CDS sequence) NM_165002.2 , NM_001273474.1
    Accession Version NM_001273474.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTCAGTT CGCTGGACGC GCTGGCCGGC AAAATAGCCA CTGCCACGCC CGGAACGGGG 
    GCGGTAACGG CGGACAGCAA GAACACACAG TCGCTGCACC ATCACCATCG CCTGGACTAT
    GATTACTCGG CGGTTGAGAT TGAACCACCG GCGGAACAAC TGGCCAATTC ACCGCGCAGC
    GAACGGGAGC GACTGCAGCA GGCCCAACAG GCCTACGAGC AGCAGGCCCA GGCGGAACTG
    GAGTTCGGTC TCGCGGAGGT GGATGCCAAG CTGACGCAGC TAAAGAGCAG CCACAGTCTG
    CAGCATCACC ACAGTCATCA CCACCAGCTG CAGCGCCATA CGGCGCTCAA TCAGCAACAG
    CAGCAGCTGC ACCACCATCA GCCCCACCAC AATCATCACC ACCATCATCC GTACCAGCGA
    CCCAGACCCA CATCGCCACC GCAACAACAG ACGTCCTCAT ACCAGCAGCA ACTTTCCCAG
    CTGGCCGCTC TGCAGGATCA CCATGATCTC CTCCAGACGC AGCAGGAGCT CTTCCACAAG
    CAGCTGGCCA CCATGCAGGA GTACCAGCGG GAAAGGGAAC GGGAACGTGA GCGGGAAAGG
    GAGCGGGAAC GTGAGCGGGA ACGGGAGCGG GATCGCAGCA GCTTCATTGA TAACAGCGAT
    TACGATTCCC AGACGGAGTT CAAAATTTGC CTGAGGGATA GTGCTTTTGG TATGAGTGAT
    AAGAGCAGCA GTGCAACGAA CAGCCTGCCG AATAGTCCCA TACACAGCAA CAACAATAAT
    CCATCGCTGC TGAACAACAA CAACAATAAC AGCAACGGCA CCAGCAGCAA CAACAGCTTA
    AACGTTGGCA ACAACAACAG CAATCCGAGC TTGGGCGGCA GCAACAGCAA CGCACTGGTT
    TCCGTAGGCA GCAACGGTAT AATGTCCGCC GCCGGTTTGG TCAACAACAA TAACAATCCG
    TGCAGCGCCA ACCGCAATGT GGTCGCCATG GTCGACGATG ACGCCTGCTT CCGTCTGGAT
    ACGGACGCCA CGGTGACGTA CGGCGAGAAG GAACCGGATC CGGACAACAT CAAGATGTTC
    GTCGGCCAGG TGCCCAAGTC GATGGACGAG TCGCAGCTGC GCGAGATGTT CGAGGAGTAC
    GGCGCCGTGC ACTCGATCAA CGTTCTGCGG GACAAGGCTA CCGGAATTAG CAAGGGTTGC
    TGCTTTGTGA CATTTTATAC GAGACACGCC GCCCTGAAAG CCCAGGATGC TTTGCATAAT
    GTTAAGACGC TAAATGGGAT GTACCATCCC ATTCAAATGA AGCCCGCCGA TAGCGAGAAT
    CGCAATGAAC GCAAACTCTT TGTGGGTATG CTAAACAAGA AGCTGAACGA GAACGACGTT
    CGCAAGCTAT TTGAAGTCCA CGGAGCCATC GAGGAGTGCA CCGTCCTTCG GGACCAAAAC
    GGACAGAGCA AGGGTTGTGC CTTCGTCACA TTCGCCACCA AGCACGCTGC CATTTCGGCC
    ATTAAAGTGA CCCTGAGCCA GAACAAGATC ATGGAGGGCT GCACATCGCC GCTGGTCGTC
    AAGTTCGCCG ATACGCAAAA AGAGAAGGAG CAGAAGAAGA TTCAGCAAAT TCAGGCCAAT
    CTCTGGAACC TGGCCAGCAA CATCAACATA CCTCTGGGCC AAACGGCCAC CAGCGTGACC
    ACGCCCATCC TGCCCAATCC GCCGCAGCAG CCTTCGCCGG TTCTCGGCGC AGATGCCATT
    ACCCCGGCCT CCATTCAACT GCTGCAGCAG CTGCAGGCGG TGGGACTGCA GCACCAGTTG
    TTGCAAGCCC TCACAGGACT GGGAGCCCAG CAGAGCAGCT CGGCGACGGA CACCAGCGCT
    GTGGCGGCCG CAGGACTTTT GACCCCGATG ACCGTCCAGA ATCTAGCGGC CATTGCGGCC
    ATGACGCAGC CGAGTCTCTC GAATGCCGCC GCGGCAGCCG CCGCTGCCAC ATCGCCTGGA
    AGCGCCCAAC TGACCAACAC GGCTGCGCTG CTCTGGTCCG ATCCGAATCC AATGGCATCG
    GCATATATGT CAACAGCCGC CGGTCTGCCG CAATTCGGGA GCGCCAGTGC ATTGTCCACT
    TCGCCGCTGG CCAGCGTTGC CCTCAGCGCC GCAGCCGCCG CCGCAGCGGG AAAGCAGATT
    GAAGGACCCG AGGGCTGCAA CCTTTTCATC TACCATCTGC CGCAGGAGTT CACCGATACG
    GATCTGGCCT CCACGTTCCT GCCCTTCGGC AATGTGATCT CGGCCAAGGT GTTCATCGAC
    AAACAGACGA GCCTGTCGAA GTGCTTCGGG TTTGTCTCCT TCGACAATCC GGACTCGGCC
    CAGGTGGCCA TAAAGGCGAT GAACGGCTTC CAGGTGGGCA CCAAGCGTCT CAAGGTGCAG
    CTGAAGAAGC CCAAGGACTC GAAGCCCTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260403.1
    CDS354..2786
    Translation

    Target ORF information:

    RefSeq Version NM_001273474.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCTCAGTT CGCTGGACGC GCTGGCCGGC AAAATAGCCA CTGCCACGCC CGGAACGGGG 
    GCGGTAACGG CGGACAGCAA GAACACACAG TCGCTGCACC ATCACCATCG CCTGGACTAT
    GATTACTCGG CGGTTGAGAT TGAACCACCG GCGGAACAAC TGGCCAATTC ACCGCGCAGC
    GAACGGGAGC GACTGCAGCA GGCCCAACAG GCCTACGAGC AGCAGGCCCA GGCGGAACTG
    GAGTTCGGTC TCGCGGAGGT GGATGCCAAG CTGACGCAGC TAAAGAGCAG CCACAGTCTG
    CAGCATCACC ACAGTCATCA CCACCAGCTG CAGCGCCATA CGGCGCTCAA TCAGCAACAG
    CAGCAGCTGC ACCACCATCA GCCCCACCAC AATCATCACC ACCATCATCC GTACCAGCGA
    CCCAGACCCA CATCGCCACC GCAACAACAG ACGTCCTCAT ACCAGCAGCA ACTTTCCCAG
    CTGGCCGCTC TGCAGGATCA CCATGATCTC CTCCAGACGC AGCAGGAGCT CTTCCACAAG
    CAGCTGGCCA CCATGCAGGA GTACCAGCGG GAAAGGGAAC GGGAACGTGA GCGGGAAAGG
    GAGCGGGAAC GTGAGCGGGA ACGGGAGCGG GATCGCAGCA GCTTCATTGA TAACAGCGAT
    TACGATTCCC AGACGGAGTT CAAAATTTGC CTGAGGGATA GTGCTTTTGG TATGAGTGAT
    AAGAGCAGCA GTGCAACGAA CAGCCTGCCG AATAGTCCCA TACACAGCAA CAACAATAAT
    CCATCGCTGC TGAACAACAA CAACAATAAC AGCAACGGCA CCAGCAGCAA CAACAGCTTA
    AACGTTGGCA ACAACAACAG CAATCCGAGC TTGGGCGGCA GCAACAGCAA CGCACTGGTT
    TCCGTAGGCA GCAACGGTAT AATGTCCGCC GCCGGTTTGG TCAACAACAA TAACAATCCG
    TGCAGCGCCA ACCGCAATGT GGTCGCCATG GTCGACGATG ACGCCTGCTT CCGTCTGGAT
    ACGGACGCCA CGGTGACGTA CGGCGAGAAG GAACCGGATC CGGACAACAT CAAGATGTTC
    GTCGGCCAGG TGCCCAAGTC GATGGACGAG TCGCAGCTGC GCGAGATGTT CGAGGAGTAC
    GGCGCCGTGC ACTCGATCAA CGTTCTGCGG GACAAGGCTA CCGGAATTAG CAAGGGTTGC
    TGCTTTGTGA CATTTTATAC GAGACACGCC GCCCTGAAAG CCCAGGATGC TTTGCATAAT
    GTTAAGACGC TAAATGGGAT GTACCATCCC ATTCAAATGA AGCCCGCCGA TAGCGAGAAT
    CGCAATGAAC GCAAACTCTT TGTGGGTATG CTAAACAAGA AGCTGAACGA GAACGACGTT
    CGCAAGCTAT TTGAAGTCCA CGGAGCCATC GAGGAGTGCA CCGTCCTTCG GGACCAAAAC
    GGACAGAGCA AGGGTTGTGC CTTCGTCACA TTCGCCACCA AGCACGCTGC CATTTCGGCC
    ATTAAAGTGA CCCTGAGCCA GAACAAGATC ATGGAGGGCT GCACATCGCC GCTGGTCGTC
    AAGTTCGCCG ATACGCAAAA AGAGAAGGAG CAGAAGAAGA TTCAGCAAAT TCAGGCCAAT
    CTCTGGAACC TGGCCAGCAA CATCAACATA CCTCTGGGCC AAACGGCCAC CAGCGTGACC
    ACGCCCATCC TGCCCAATCC GCCGCAGCAG CCTTCGCCGG TTCTCGGCGC AGATGCCATT
    ACCCCGGCCT CCATTCAACT GCTGCAGCAG CTGCAGGCGG TGGGACTGCA GCACCAGTTG
    TTGCAAGCCC TCACAGGACT GGGAGCCCAG CAGAGCAGCT CGGCGACGGA CACCAGCGCT
    GTGGCGGCCG CAGGACTTTT GACCCCGATG ACCGTCCAGA ATCTAGCGGC CATTGCGGCC
    ATGACGCAGC CGAGTCTCTC GAATGCCGCC GCGGCAGCCG CCGCTGCCAC ATCGCCTGGA
    AGCGCCCAAC TGACCAACAC GGCTGCGCTG CTCTGGTCCG ATCCGAATCC AATGGCATCG
    GCATATATGT CAACAGCCGC CGGTCTGCCG CAATTCGGGA GCGCCAGTGC ATTGTCCACT
    TCGCCGCTGG CCAGCGTTGC CCTCAGCGCC GCAGCCGCCG CCGCAGCGGG AAAGCAGATT
    GAAGGACCCG AGGGCTGCAA CCTTTTCATC TACCATCTGC CGCAGGAGTT CACCGATACG
    GATCTGGCCT CCACGTTCCT GCCCTTCGGC AATGTGATCT CGGCCAAGGT GTTCATCGAC
    AAACAGACGA GCCTGTCGAA GTGCTTCGGG TTTGTCTCCT TCGACAATCC GGACTCGGCC
    CAGGTGGCCA TAAAGGCGAT GAACGGCTTC CAGGTGGGCA CCAAGCGTCT CAAGGTGCAG
    CTGAAGAAGC CCAAGGACTC GAAGCCCTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14524
    Clone ID Related Accession (Same CDS sequence) NM_165003.3
    Accession Version NM_165003.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165003.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTTCACCA GCCGCGCTTC GTTTTTGGCC AATCGCCGCA TGATTTTCGA TTTCTCCGAG 
    AAGAACAACG ATATCTACTT GGATGCTTTT GGTATGAGTG ATAAGAGCAG CAGTGCAACG
    AACAGCCTGC CGAATAGTCC CATACACAGC AACAACAATA ATCCATCGCT GCTGAACAAC
    AACAACAATA ACAGCAACGG CACCAGCAGC AACAACAGCT TAAACGTTGG CAACAACAAC
    AGCAATCCGA GCTTGGGCGG CAGCAACAGC AACGCACTGG TTTCCGTAGG CAGCAACGGT
    ATAATGTCCG CCGCCGGTTT GGTCAACAAC AATAACAATC CGTGCAGCGC CAACCGCAAT
    GTGGTCGCCA TGGTCGACGA TGACGCCTGC TTCCGTCTGG ATACGGACGC CACGGTGACG
    TACGGCGAGA AGGAACCGGA TCCGGACAAC ATCAAGATGT TCGTCGGCCA GGTGCCCAAG
    TCGATGGACG AGTCGCAGCT GCGCGAGATG TTCGAGGAGT ACGGCGCCGT GCACTCGATC
    AACGTTCTGC GGGACAAGGC TACCGGAATT AGCAAGGGTT GCTGCTTTGT GACATTTTAT
    ACGAGACACG CCGCCCTGAA AGCCCAGGAT GCTTTGCATA ATGTTAAGAC GCTAAATGGG
    ATGTACCATC CCATTCAAAT GAAGCCCGCC GATAGCGAGA ATCGCAATGA ACGCAAACTC
    TTTGTGGGTA TGCTAAACAA GAAGCTGAAC GAGAACGACG TTCGCAAGCT ATTTGAAGTC
    CACGGAGCCA TCGAGGAGTG CACCGTCCTT CGGGACCAAA ACGGACAGAG CAAGGGTTGT
    GCCTTCGTCA CATTCGCCAC CAAGCACGCT GCCATTTCGG CCATTAAAGT GACCCTGAGC
    CAGAACAAGA TCATGGAGGG CTGCACATCG CCGCTGGTCG TCAAGTTCGC CGATACGCAA
    AAAGAGAAGG AGCAGAAGAA GATTCAGCAA ATTCAGGCCA ATCTCTGGAA CCTGGCCAGC
    AACATCAACA TACCTCTGGG CCAAACGGCC ACCAGCGTGA CCACGCCCAT CCTGCCCAAT
    CCGCCGCAGC AGCCTTCGCC GGTTCTCGGC GCAGATGCCA TTACCCCGGC CTCCATTCAA
    CTGCTGCAGC AGCTGCAGGC GGTGGGACTG CAGCACCAGT TGTTGCAAGC CCTCACAGGA
    CTGGGAGCCC AGCAGAGCAG CTCGGCGACG GACACCAGCG CTGTGGCGGC CGCAGGACTT
    TTGACCCCGA TGACCGTCCA GAATCTAGCG GCCATTGCGG CCATGACGCA GCCGAGTCTC
    TCGAATGCCG CCGCGGCAGC CGCCGCTGCC ACATCGCCTG GAAGCGCCCA ACTGACCAAC
    ACGGCTGCGC TGCTCTGCCT TTTGGGTAAA AAGGCTGCAG CTAAAAAATC AGCTAAAAAG
    ACAGGGTCCG ATCCGAATCC AATGGCATCG GCATATATGT CAACAGCCGC CGGTCTGCCG
    CAATTCGGGA GCGCCAGTGC ATTGTCCACT TCGCCGCTGG CCAGCGTTGC CCTCAGCGCC
    GCAGCCGCCG CCGCAGCGGG AAAGCAGATT GAAGGACCCG AGGGCTGCAA CCTTTTCATC
    TACCATCTGC CGCAGGAGTT CACCGATACG GATCTGGCCT CCACGTTCCT GCCCTTCGGC
    AATGTGATCT CGGCCAAGGT GTTCATCGAC AAACAGACGA GCCTGTCGAA GTGCTTCGGG
    TTTGTCTCCT TCGACAATCC GGACTCGGCC CAGGTGGCCA TAAAGGCGAT GAACGGCTTC
    CAGGTGGGCA CCAAGCGTCT CAAGGTGCAG CTGAAGAAGC CCAAGGACTC GAAGCCCTAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723738.2
    CDS334..2196
    Translation

    Target ORF information:

    RefSeq Version NM_165003.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165003.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTTCACCA GCCGCGCTTC GTTTTTGGCC AATCGCCGCA TGATTTTCGA TTTCTCCGAG 
    AAGAACAACG ATATCTACTT GGATGCTTTT GGTATGAGTG ATAAGAGCAG CAGTGCAACG
    AACAGCCTGC CGAATAGTCC CATACACAGC AACAACAATA ATCCATCGCT GCTGAACAAC
    AACAACAATA ACAGCAACGG CACCAGCAGC AACAACAGCT TAAACGTTGG CAACAACAAC
    AGCAATCCGA GCTTGGGCGG CAGCAACAGC AACGCACTGG TTTCCGTAGG CAGCAACGGT
    ATAATGTCCG CCGCCGGTTT GGTCAACAAC AATAACAATC CGTGCAGCGC CAACCGCAAT
    GTGGTCGCCA TGGTCGACGA TGACGCCTGC TTCCGTCTGG ATACGGACGC CACGGTGACG
    TACGGCGAGA AGGAACCGGA TCCGGACAAC ATCAAGATGT TCGTCGGCCA GGTGCCCAAG
    TCGATGGACG AGTCGCAGCT GCGCGAGATG TTCGAGGAGT ACGGCGCCGT GCACTCGATC
    AACGTTCTGC GGGACAAGGC TACCGGAATT AGCAAGGGTT GCTGCTTTGT GACATTTTAT
    ACGAGACACG CCGCCCTGAA AGCCCAGGAT GCTTTGCATA ATGTTAAGAC GCTAAATGGG
    ATGTACCATC CCATTCAAAT GAAGCCCGCC GATAGCGAGA ATCGCAATGA ACGCAAACTC
    TTTGTGGGTA TGCTAAACAA GAAGCTGAAC GAGAACGACG TTCGCAAGCT ATTTGAAGTC
    CACGGAGCCA TCGAGGAGTG CACCGTCCTT CGGGACCAAA ACGGACAGAG CAAGGGTTGT
    GCCTTCGTCA CATTCGCCAC CAAGCACGCT GCCATTTCGG CCATTAAAGT GACCCTGAGC
    CAGAACAAGA TCATGGAGGG CTGCACATCG CCGCTGGTCG TCAAGTTCGC CGATACGCAA
    AAAGAGAAGG AGCAGAAGAA GATTCAGCAA ATTCAGGCCA ATCTCTGGAA CCTGGCCAGC
    AACATCAACA TACCTCTGGG CCAAACGGCC ACCAGCGTGA CCACGCCCAT CCTGCCCAAT
    CCGCCGCAGC AGCCTTCGCC GGTTCTCGGC GCAGATGCCA TTACCCCGGC CTCCATTCAA
    CTGCTGCAGC AGCTGCAGGC GGTGGGACTG CAGCACCAGT TGTTGCAAGC CCTCACAGGA
    CTGGGAGCCC AGCAGAGCAG CTCGGCGACG GACACCAGCG CTGTGGCGGC CGCAGGACTT
    TTGACCCCGA TGACCGTCCA GAATCTAGCG GCCATTGCGG CCATGACGCA GCCGAGTCTC
    TCGAATGCCG CCGCGGCAGC CGCCGCTGCC ACATCGCCTG GAAGCGCCCA ACTGACCAAC
    ACGGCTGCGC TGCTCTGCCT TTTGGGTAAA AAGGCTGCAG CTAAAAAATC AGCTAAAAAG
    ACAGGGTCCG ATCCGAATCC AATGGCATCG GCATATATGT CAACAGCCGC CGGTCTGCCG
    CAATTCGGGA GCGCCAGTGC ATTGTCCACT TCGCCGCTGG CCAGCGTTGC CCTCAGCGCC
    GCAGCCGCCG CCGCAGCGGG AAAGCAGATT GAAGGACCCG AGGGCTGCAA CCTTTTCATC
    TACCATCTGC CGCAGGAGTT CACCGATACG GATCTGGCCT CCACGTTCCT GCCCTTCGGC
    AATGTGATCT CGGCCAAGGT GTTCATCGAC AAACAGACGA GCCTGTCGAA GTGCTTCGGG
    TTTGTCTCCT TCGACAATCC GGACTCGGCC CAGGTGGCCA TAAAGGCGAT GAACGGCTTC
    CAGGTGGGCA CCAAGCGTCT CAAGGTGCAG CTGAAGAAGC CCAAGGACTC GAAGCCCTAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14525
    Clone ID Related Accession (Same CDS sequence) NM_001169492.2 , NM_165004.3
    Accession Version NM_001169492.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169492.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTCACCA GCCGCGCTTC GTTTTTGGCC AATCGCCGCA TGATTTTCGA TTTCTCCGAG 
    AAGAACAACG ATATCTACTT GGATGCTTTT GGTATGAGTG ATAAGAGCAG CAGTGCAACG
    AACAGCCTGC CGAATAGTCC CATACACAGC AACAACAATA ATCCATCGCT GCTGAACAAC
    AACAACAATA ACAGCAACGG CACCAGCAGC AACAACAGCT TAAACGTTGG CAACAACAAC
    AGCAATCCGA GCTTGGGCGG CAGCAACAGC AACGCACTGG TTTCCGTAGG CAGCAACGGT
    ATAATGTCCG CCGCCGGTTT GGTCAACAAC AATAACAATC CGTGCAGCGC CAACCGCAAT
    GTGGTCGCCA TGGTCGACGA TGACGCCTGC TTCCGTCTGG ATACGGACGC CACGGTGACG
    TACGGCGAGA AGGAACCGGA TCCGGACAAC ATCAAGATGT TCGTCGGCCA GGTGCCCAAG
    TCGATGGACG AGTCGCAGCT GCGCGAGATG TTCGAGGAGT ACGGCGCCGT GCACTCGATC
    AACGTTCTGC GGGACAAGGC TACCGGAATT AGCAAGGGTT GCTGCTTTGT GACATTTTAT
    ACGAGACACG CCGCCCTGAA AGCCCAGGAT GCTTTGCATA ATGTTAAGAC GCTAAATGGG
    ATGTACCATC CCATTCAAAT GAAGCCCGCC GATAGCGAGA ATCGCAATGA ACGCAAACTC
    TTTGTGGGTA TGCTAAACAA GAAGCTGAAC GAGAACGACG TTCGCAAGCT ATTTGAAGTC
    CACGGAGCCA TCGAGGAGTG CACCGTCCTT CGGGACCAAA ACGGACAGAG CAAGGGTTGT
    GCCTTCGTCA CATTCGCCAC CAAGCACGCT GCCATTTCGG CCATTAAAGT GACCCTGAGC
    CAGAACAAGA TCATGGAGGG CTGCACATCG CCGCTGGTCG TCAAGTTCGC CGATACGCAA
    AAAGAGAAGG AGCAGAAGAA GATTCAGCAA ATTCAGGCCA ATCTCTGGAA CCTGGCCAGC
    AACATCAACA TACCTCTGGG CCAAACGGCC ACCAGCGTGA CCACGCCCAT CCTGCCCAAT
    CCGCCGCAGC AGCCTTCGCC GGTTCTCGGC GCAGATGCCA TTACCCCGGC CTCCATTCAA
    CTGCTGCAGC AGCTGCAGGC GGTGGGACTG CAGCACCAGT TGTTGCAAGC CCTCACAGGA
    CTGGGAGCCC AGCAGAGCAG CTCGGCGACG GACACCAGCG CTGTGGCGGC CGCAGGACTT
    TTGACCCCGA TGACCGTCCA GAATCTAGCG GCCATTGCGG CCATGACGCA GCCGAGTCTC
    TCGAATGCCG CCGCGGCAGC CGCCGCTGCC ACATCGCCTG GAAGCGCCCA ACTGACCAAC
    ACGGCTGCGC TGCTCTGGTC CGATCCGAAT CCAATGGCAT CGGCATATAT GTCAACAGCC
    GCCGGTCTGC CGCAATTCGG GAGCGCCAGT GCATTGTCCA CTTCGCCGCT GGCCAGCGTT
    GCCCTCAGCG CCGCAGCCGC CGCCGCAGCG GGAAAGCAGA TTGAAGGACC CGAGGGCTGC
    AACCTTTTCA TCTACCATCT GCCGCAGGAG TTCACCGATA CGGATCTGGC CTCCACGTTC
    CTGCCCTTCG GCAATGTGAT CTCGGCCAAG GTGTTCATCG ACAAACAGAC GAGCCTGTCG
    AAGTGCTTCG GGTTTGTCTC CTTCGACAAT CCGGACTCGG CCCAGGTGGC CATAAAGGCG
    ATGAACGGCT TCCAGGTGGG CACCAAGCGT CTCAAGGTGC AGCTGAAGAA GCCCAAGGAC
    TCGAAGCCCT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162963.1
    CDS334..2148
    Translation

    Target ORF information:

    RefSeq Version NM_001169492.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169492.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTCACCA GCCGCGCTTC GTTTTTGGCC AATCGCCGCA TGATTTTCGA TTTCTCCGAG 
    AAGAACAACG ATATCTACTT GGATGCTTTT GGTATGAGTG ATAAGAGCAG CAGTGCAACG
    AACAGCCTGC CGAATAGTCC CATACACAGC AACAACAATA ATCCATCGCT GCTGAACAAC
    AACAACAATA ACAGCAACGG CACCAGCAGC AACAACAGCT TAAACGTTGG CAACAACAAC
    AGCAATCCGA GCTTGGGCGG CAGCAACAGC AACGCACTGG TTTCCGTAGG CAGCAACGGT
    ATAATGTCCG CCGCCGGTTT GGTCAACAAC AATAACAATC CGTGCAGCGC CAACCGCAAT
    GTGGTCGCCA TGGTCGACGA TGACGCCTGC TTCCGTCTGG ATACGGACGC CACGGTGACG
    TACGGCGAGA AGGAACCGGA TCCGGACAAC ATCAAGATGT TCGTCGGCCA GGTGCCCAAG
    TCGATGGACG AGTCGCAGCT GCGCGAGATG TTCGAGGAGT ACGGCGCCGT GCACTCGATC
    AACGTTCTGC GGGACAAGGC TACCGGAATT AGCAAGGGTT GCTGCTTTGT GACATTTTAT
    ACGAGACACG CCGCCCTGAA AGCCCAGGAT GCTTTGCATA ATGTTAAGAC GCTAAATGGG
    ATGTACCATC CCATTCAAAT GAAGCCCGCC GATAGCGAGA ATCGCAATGA ACGCAAACTC
    TTTGTGGGTA TGCTAAACAA GAAGCTGAAC GAGAACGACG TTCGCAAGCT ATTTGAAGTC
    CACGGAGCCA TCGAGGAGTG CACCGTCCTT CGGGACCAAA ACGGACAGAG CAAGGGTTGT
    GCCTTCGTCA CATTCGCCAC CAAGCACGCT GCCATTTCGG CCATTAAAGT GACCCTGAGC
    CAGAACAAGA TCATGGAGGG CTGCACATCG CCGCTGGTCG TCAAGTTCGC CGATACGCAA
    AAAGAGAAGG AGCAGAAGAA GATTCAGCAA ATTCAGGCCA ATCTCTGGAA CCTGGCCAGC
    AACATCAACA TACCTCTGGG CCAAACGGCC ACCAGCGTGA CCACGCCCAT CCTGCCCAAT
    CCGCCGCAGC AGCCTTCGCC GGTTCTCGGC GCAGATGCCA TTACCCCGGC CTCCATTCAA
    CTGCTGCAGC AGCTGCAGGC GGTGGGACTG CAGCACCAGT TGTTGCAAGC CCTCACAGGA
    CTGGGAGCCC AGCAGAGCAG CTCGGCGACG GACACCAGCG CTGTGGCGGC CGCAGGACTT
    TTGACCCCGA TGACCGTCCA GAATCTAGCG GCCATTGCGG CCATGACGCA GCCGAGTCTC
    TCGAATGCCG CCGCGGCAGC CGCCGCTGCC ACATCGCCTG GAAGCGCCCA ACTGACCAAC
    ACGGCTGCGC TGCTCTGGTC CGATCCGAAT CCAATGGCAT CGGCATATAT GTCAACAGCC
    GCCGGTCTGC CGCAATTCGG GAGCGCCAGT GCATTGTCCA CTTCGCCGCT GGCCAGCGTT
    GCCCTCAGCG CCGCAGCCGC CGCCGCAGCG GGAAAGCAGA TTGAAGGACC CGAGGGCTGC
    AACCTTTTCA TCTACCATCT GCCGCAGGAG TTCACCGATA CGGATCTGGC CTCCACGTTC
    CTGCCCTTCG GCAATGTGAT CTCGGCCAAG GTGTTCATCG ACAAACAGAC GAGCCTGTCG
    AAGTGCTTCG GGTTTGTCTC CTTCGACAAT CCGGACTCGG CCCAGGTGGC CATAAAGGCG
    ATGAACGGCT TCCAGGTGGG CACCAAGCGT CTCAAGGTGC AGCTGAAGAA GCCCAAGGAC
    TCGAAGCCCT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14525
    Clone ID Related Accession (Same CDS sequence) NM_001169492.2 , NM_165004.3
    Accession Version NM_165004.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165004.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTCACCA GCCGCGCTTC GTTTTTGGCC AATCGCCGCA TGATTTTCGA TTTCTCCGAG 
    AAGAACAACG ATATCTACTT GGATGCTTTT GGTATGAGTG ATAAGAGCAG CAGTGCAACG
    AACAGCCTGC CGAATAGTCC CATACACAGC AACAACAATA ATCCATCGCT GCTGAACAAC
    AACAACAATA ACAGCAACGG CACCAGCAGC AACAACAGCT TAAACGTTGG CAACAACAAC
    AGCAATCCGA GCTTGGGCGG CAGCAACAGC AACGCACTGG TTTCCGTAGG CAGCAACGGT
    ATAATGTCCG CCGCCGGTTT GGTCAACAAC AATAACAATC CGTGCAGCGC CAACCGCAAT
    GTGGTCGCCA TGGTCGACGA TGACGCCTGC TTCCGTCTGG ATACGGACGC CACGGTGACG
    TACGGCGAGA AGGAACCGGA TCCGGACAAC ATCAAGATGT TCGTCGGCCA GGTGCCCAAG
    TCGATGGACG AGTCGCAGCT GCGCGAGATG TTCGAGGAGT ACGGCGCCGT GCACTCGATC
    AACGTTCTGC GGGACAAGGC TACCGGAATT AGCAAGGGTT GCTGCTTTGT GACATTTTAT
    ACGAGACACG CCGCCCTGAA AGCCCAGGAT GCTTTGCATA ATGTTAAGAC GCTAAATGGG
    ATGTACCATC CCATTCAAAT GAAGCCCGCC GATAGCGAGA ATCGCAATGA ACGCAAACTC
    TTTGTGGGTA TGCTAAACAA GAAGCTGAAC GAGAACGACG TTCGCAAGCT ATTTGAAGTC
    CACGGAGCCA TCGAGGAGTG CACCGTCCTT CGGGACCAAA ACGGACAGAG CAAGGGTTGT
    GCCTTCGTCA CATTCGCCAC CAAGCACGCT GCCATTTCGG CCATTAAAGT GACCCTGAGC
    CAGAACAAGA TCATGGAGGG CTGCACATCG CCGCTGGTCG TCAAGTTCGC CGATACGCAA
    AAAGAGAAGG AGCAGAAGAA GATTCAGCAA ATTCAGGCCA ATCTCTGGAA CCTGGCCAGC
    AACATCAACA TACCTCTGGG CCAAACGGCC ACCAGCGTGA CCACGCCCAT CCTGCCCAAT
    CCGCCGCAGC AGCCTTCGCC GGTTCTCGGC GCAGATGCCA TTACCCCGGC CTCCATTCAA
    CTGCTGCAGC AGCTGCAGGC GGTGGGACTG CAGCACCAGT TGTTGCAAGC CCTCACAGGA
    CTGGGAGCCC AGCAGAGCAG CTCGGCGACG GACACCAGCG CTGTGGCGGC CGCAGGACTT
    TTGACCCCGA TGACCGTCCA GAATCTAGCG GCCATTGCGG CCATGACGCA GCCGAGTCTC
    TCGAATGCCG CCGCGGCAGC CGCCGCTGCC ACATCGCCTG GAAGCGCCCA ACTGACCAAC
    ACGGCTGCGC TGCTCTGGTC CGATCCGAAT CCAATGGCAT CGGCATATAT GTCAACAGCC
    GCCGGTCTGC CGCAATTCGG GAGCGCCAGT GCATTGTCCA CTTCGCCGCT GGCCAGCGTT
    GCCCTCAGCG CCGCAGCCGC CGCCGCAGCG GGAAAGCAGA TTGAAGGACC CGAGGGCTGC
    AACCTTTTCA TCTACCATCT GCCGCAGGAG TTCACCGATA CGGATCTGGC CTCCACGTTC
    CTGCCCTTCG GCAATGTGAT CTCGGCCAAG GTGTTCATCG ACAAACAGAC GAGCCTGTCG
    AAGTGCTTCG GGTTTGTCTC CTTCGACAAT CCGGACTCGG CCCAGGTGGC CATAAAGGCG
    ATGAACGGCT TCCAGGTGGG CACCAAGCGT CTCAAGGTGC AGCTGAAGAA GCCCAAGGAC
    TCGAAGCCCT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723739.1
    CDS334..2148
    Translation

    Target ORF information:

    RefSeq Version NM_165004.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165004.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTCACCA GCCGCGCTTC GTTTTTGGCC AATCGCCGCA TGATTTTCGA TTTCTCCGAG 
    AAGAACAACG ATATCTACTT GGATGCTTTT GGTATGAGTG ATAAGAGCAG CAGTGCAACG
    AACAGCCTGC CGAATAGTCC CATACACAGC AACAACAATA ATCCATCGCT GCTGAACAAC
    AACAACAATA ACAGCAACGG CACCAGCAGC AACAACAGCT TAAACGTTGG CAACAACAAC
    AGCAATCCGA GCTTGGGCGG CAGCAACAGC AACGCACTGG TTTCCGTAGG CAGCAACGGT
    ATAATGTCCG CCGCCGGTTT GGTCAACAAC AATAACAATC CGTGCAGCGC CAACCGCAAT
    GTGGTCGCCA TGGTCGACGA TGACGCCTGC TTCCGTCTGG ATACGGACGC CACGGTGACG
    TACGGCGAGA AGGAACCGGA TCCGGACAAC ATCAAGATGT TCGTCGGCCA GGTGCCCAAG
    TCGATGGACG AGTCGCAGCT GCGCGAGATG TTCGAGGAGT ACGGCGCCGT GCACTCGATC
    AACGTTCTGC GGGACAAGGC TACCGGAATT AGCAAGGGTT GCTGCTTTGT GACATTTTAT
    ACGAGACACG CCGCCCTGAA AGCCCAGGAT GCTTTGCATA ATGTTAAGAC GCTAAATGGG
    ATGTACCATC CCATTCAAAT GAAGCCCGCC GATAGCGAGA ATCGCAATGA ACGCAAACTC
    TTTGTGGGTA TGCTAAACAA GAAGCTGAAC GAGAACGACG TTCGCAAGCT ATTTGAAGTC
    CACGGAGCCA TCGAGGAGTG CACCGTCCTT CGGGACCAAA ACGGACAGAG CAAGGGTTGT
    GCCTTCGTCA CATTCGCCAC CAAGCACGCT GCCATTTCGG CCATTAAAGT GACCCTGAGC
    CAGAACAAGA TCATGGAGGG CTGCACATCG CCGCTGGTCG TCAAGTTCGC CGATACGCAA
    AAAGAGAAGG AGCAGAAGAA GATTCAGCAA ATTCAGGCCA ATCTCTGGAA CCTGGCCAGC
    AACATCAACA TACCTCTGGG CCAAACGGCC ACCAGCGTGA CCACGCCCAT CCTGCCCAAT
    CCGCCGCAGC AGCCTTCGCC GGTTCTCGGC GCAGATGCCA TTACCCCGGC CTCCATTCAA
    CTGCTGCAGC AGCTGCAGGC GGTGGGACTG CAGCACCAGT TGTTGCAAGC CCTCACAGGA
    CTGGGAGCCC AGCAGAGCAG CTCGGCGACG GACACCAGCG CTGTGGCGGC CGCAGGACTT
    TTGACCCCGA TGACCGTCCA GAATCTAGCG GCCATTGCGG CCATGACGCA GCCGAGTCTC
    TCGAATGCCG CCGCGGCAGC CGCCGCTGCC ACATCGCCTG GAAGCGCCCA ACTGACCAAC
    ACGGCTGCGC TGCTCTGGTC CGATCCGAAT CCAATGGCAT CGGCATATAT GTCAACAGCC
    GCCGGTCTGC CGCAATTCGG GAGCGCCAGT GCATTGTCCA CTTCGCCGCT GGCCAGCGTT
    GCCCTCAGCG CCGCAGCCGC CGCCGCAGCG GGAAAGCAGA TTGAAGGACC CGAGGGCTGC
    AACCTTTTCA TCTACCATCT GCCGCAGGAG TTCACCGATA CGGATCTGGC CTCCACGTTC
    CTGCCCTTCG GCAATGTGAT CTCGGCCAAG GTGTTCATCG ACAAACAGAC GAGCCTGTCG
    AAGTGCTTCG GGTTTGTCTC CTTCGACAAT CCGGACTCGG CCCAGGTGGC CATAAAGGCG
    ATGAACGGCT TCCAGGTGGG CACCAAGCGT CTCAAGGTGC AGCTGAAGAA GCCCAAGGAC
    TCGAAGCCCT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16707
    Clone ID Related Accession (Same CDS sequence) NM_001273476.1 , NM_001273477.1 , NM_001273478.1 , NM_001273475.1
    Accession Version NM_001273476.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGTGATA AGAGCAGCAG TGCAACGAAC AGCCTGCCGA ATAGTCCCAT ACACAGCAAC 
    AACAATAATC CATCGCTGCT GAACAACAAC AACAATAACA GCAACGGCAC CAGCAGCAAC
    AACAGCTTAA ACGTTGGCAA CAACAACAGC AATCCGAGCT TGGGCGGCAG CAACAGCAAC
    GCACTGGTTT CCGTAGGCAG CAACGGTATA ATGTCCGCCG CCGGTTTGGT CAACAACAAT
    AACAATCCGT GCAGCGCCAA CCGCAATGTG GTCGCCATGG TCGACGATGA CGCCTGCTTC
    CGTCTGGATA CGGACGCCAC GGTGACGTAC GGCGAGAAGG AACCGGATCC GGACAACATC
    AAGATGTTCG TCGGCCAGGT GCCCAAGTCG ATGGACGAGT CGCAGCTGCG CGAGATGTTC
    GAGGAGTACG GCGCCGTGCA CTCGATCAAC GTTCTGCGGG ACAAGGCTAC CGGAATTAGC
    AAGGGTTGCT GCTTTGTGAC ATTTTATACG AGACACGCCG CCCTGAAAGC CCAGGATGCT
    TTGCATAATG TTAAGACGCT AAATGGGATG TACCATCCCA TTCAAATGAA GCCCGCCGAT
    AGCGAGAATC GCAATGAACG CAAACTCTTT GTGGGTATGC TAAACAAGAA GCTGAACGAG
    AACGACGTTC GCAAGCTATT TGAAGTCCAC GGAGCCATCG AGGAGTGCAC CGTCCTTCGG
    GACCAAAACG GACAGAGCAA GGGTTGTGCC TTCGTCACAT TCGCCACCAA GCACGCTGCC
    ATTTCGGCCA TTAAAGTGAC CCTGAGCCAG AACAAGATCA TGGAGGGCTG CACATCGCCG
    CTGGTCGTCA AGTTCGCCGA TACGCAAAAA GAGAAGGAGC AGAAGAAGAT TCAGCAAATT
    CAGGCCAATC TCTGGAACCT GGCCAGCAAC ATCAACATAC CTCTGGGCCA AACGGCCACC
    AGCGTGACCA CGCCCATCCT GCCCAATCCG CCGCAGCAGC CTTCGCCGGT TCTCGGCGCA
    GATGCCATTA CCCCGGCCTC CATTCAACTG CTGCAGCAGC TGCAGGCGGT GGGACTGCAG
    CACCAGTTGT TGCAAGCCCT CACAGGACTG GGAGCCCAGC AGAGCAGCTC GGCGACGGAC
    ACCAGCGCTG TGGCGGCCGC AGGACTTTTG ACCCCGATGA CCGTCCAGAA TCTAGCGGCC
    ATTGCGGCCA TGACGCAGCC GAGTCTCTCG AATGCCGCCG CGGCAGCCGC CGCTGCCACA
    TCGCCTGGAA GCGCCCAACT GACCAACACG GCTGCGCTGC TCTGGTCCGA TCCGAATCCA
    ATGGCATCGG CATATATGTC AACAGCCGCC GGTCTGCCGC AATTCGGGAG CGCCAGTGCA
    TTGTCCACTT CGCCGCTGGC CAGCGTTGCC CTCAGCGCCG CAGCCGCCGC CGCAGCGGGA
    AAGCAGATTG AAGGACCCGA GGGCTGCAAC CTTTTCATCT ACCATCTGCC GCAGGAGTTC
    ACCGATACGG ATCTGGCCTC CACGTTCCTG CCCTTCGGCA ATGTGATCTC GGCCAAGGTG
    TTCATCGACA AACAGACGAG CCTGTCGAAG TGCTTCGGGT TTGTCTCCTT CGACAATCCG
    GACTCGGCCC AGGTGGCCAT AAAGGCGATG AACGGCTTCC AGGTGGGCAC CAAGCGTCTC
    AAGGTGCAGC TGAAGAAGCC CAAGGACTCG AAGCCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260405.1
    CDS152..1873
    Translation

    Target ORF information:

    RefSeq Version NM_001273476.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273476.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGTGATA AGAGCAGCAG TGCAACGAAC AGCCTGCCGA ATAGTCCCAT ACACAGCAAC 
    AACAATAATC CATCGCTGCT GAACAACAAC AACAATAACA GCAACGGCAC CAGCAGCAAC
    AACAGCTTAA ACGTTGGCAA CAACAACAGC AATCCGAGCT TGGGCGGCAG CAACAGCAAC
    GCACTGGTTT CCGTAGGCAG CAACGGTATA ATGTCCGCCG CCGGTTTGGT CAACAACAAT
    AACAATCCGT GCAGCGCCAA CCGCAATGTG GTCGCCATGG TCGACGATGA CGCCTGCTTC
    CGTCTGGATA CGGACGCCAC GGTGACGTAC GGCGAGAAGG AACCGGATCC GGACAACATC
    AAGATGTTCG TCGGCCAGGT GCCCAAGTCG ATGGACGAGT CGCAGCTGCG CGAGATGTTC
    GAGGAGTACG GCGCCGTGCA CTCGATCAAC GTTCTGCGGG ACAAGGCTAC CGGAATTAGC
    AAGGGTTGCT GCTTTGTGAC ATTTTATACG AGACACGCCG CCCTGAAAGC CCAGGATGCT
    TTGCATAATG TTAAGACGCT AAATGGGATG TACCATCCCA TTCAAATGAA GCCCGCCGAT
    AGCGAGAATC GCAATGAACG CAAACTCTTT GTGGGTATGC TAAACAAGAA GCTGAACGAG
    AACGACGTTC GCAAGCTATT TGAAGTCCAC GGAGCCATCG AGGAGTGCAC CGTCCTTCGG
    GACCAAAACG GACAGAGCAA GGGTTGTGCC TTCGTCACAT TCGCCACCAA GCACGCTGCC
    ATTTCGGCCA TTAAAGTGAC CCTGAGCCAG AACAAGATCA TGGAGGGCTG CACATCGCCG
    CTGGTCGTCA AGTTCGCCGA TACGCAAAAA GAGAAGGAGC AGAAGAAGAT TCAGCAAATT
    CAGGCCAATC TCTGGAACCT GGCCAGCAAC ATCAACATAC CTCTGGGCCA AACGGCCACC
    AGCGTGACCA CGCCCATCCT GCCCAATCCG CCGCAGCAGC CTTCGCCGGT TCTCGGCGCA
    GATGCCATTA CCCCGGCCTC CATTCAACTG CTGCAGCAGC TGCAGGCGGT GGGACTGCAG
    CACCAGTTGT TGCAAGCCCT CACAGGACTG GGAGCCCAGC AGAGCAGCTC GGCGACGGAC
    ACCAGCGCTG TGGCGGCCGC AGGACTTTTG ACCCCGATGA CCGTCCAGAA TCTAGCGGCC
    ATTGCGGCCA TGACGCAGCC GAGTCTCTCG AATGCCGCCG CGGCAGCCGC CGCTGCCACA
    TCGCCTGGAA GCGCCCAACT GACCAACACG GCTGCGCTGC TCTGGTCCGA TCCGAATCCA
    ATGGCATCGG CATATATGTC AACAGCCGCC GGTCTGCCGC AATTCGGGAG CGCCAGTGCA
    TTGTCCACTT CGCCGCTGGC CAGCGTTGCC CTCAGCGCCG CAGCCGCCGC CGCAGCGGGA
    AAGCAGATTG AAGGACCCGA GGGCTGCAAC CTTTTCATCT ACCATCTGCC GCAGGAGTTC
    ACCGATACGG ATCTGGCCTC CACGTTCCTG CCCTTCGGCA ATGTGATCTC GGCCAAGGTG
    TTCATCGACA AACAGACGAG CCTGTCGAAG TGCTTCGGGT TTGTCTCCTT CGACAATCCG
    GACTCGGCCC AGGTGGCCAT AAAGGCGATG AACGGCTTCC AGGTGGGCAC CAAGCGTCTC
    AAGGTGCAGC TGAAGAAGCC CAAGGACTCG AAGCCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16707
    Clone ID Related Accession (Same CDS sequence) NM_001273476.1 , NM_001273477.1 , NM_001273478.1 , NM_001273475.1
    Accession Version NM_001273477.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGTGATA AGAGCAGCAG TGCAACGAAC AGCCTGCCGA ATAGTCCCAT ACACAGCAAC 
    AACAATAATC CATCGCTGCT GAACAACAAC AACAATAACA GCAACGGCAC CAGCAGCAAC
    AACAGCTTAA ACGTTGGCAA CAACAACAGC AATCCGAGCT TGGGCGGCAG CAACAGCAAC
    GCACTGGTTT CCGTAGGCAG CAACGGTATA ATGTCCGCCG CCGGTTTGGT CAACAACAAT
    AACAATCCGT GCAGCGCCAA CCGCAATGTG GTCGCCATGG TCGACGATGA CGCCTGCTTC
    CGTCTGGATA CGGACGCCAC GGTGACGTAC GGCGAGAAGG AACCGGATCC GGACAACATC
    AAGATGTTCG TCGGCCAGGT GCCCAAGTCG ATGGACGAGT CGCAGCTGCG CGAGATGTTC
    GAGGAGTACG GCGCCGTGCA CTCGATCAAC GTTCTGCGGG ACAAGGCTAC CGGAATTAGC
    AAGGGTTGCT GCTTTGTGAC ATTTTATACG AGACACGCCG CCCTGAAAGC CCAGGATGCT
    TTGCATAATG TTAAGACGCT AAATGGGATG TACCATCCCA TTCAAATGAA GCCCGCCGAT
    AGCGAGAATC GCAATGAACG CAAACTCTTT GTGGGTATGC TAAACAAGAA GCTGAACGAG
    AACGACGTTC GCAAGCTATT TGAAGTCCAC GGAGCCATCG AGGAGTGCAC CGTCCTTCGG
    GACCAAAACG GACAGAGCAA GGGTTGTGCC TTCGTCACAT TCGCCACCAA GCACGCTGCC
    ATTTCGGCCA TTAAAGTGAC CCTGAGCCAG AACAAGATCA TGGAGGGCTG CACATCGCCG
    CTGGTCGTCA AGTTCGCCGA TACGCAAAAA GAGAAGGAGC AGAAGAAGAT TCAGCAAATT
    CAGGCCAATC TCTGGAACCT GGCCAGCAAC ATCAACATAC CTCTGGGCCA AACGGCCACC
    AGCGTGACCA CGCCCATCCT GCCCAATCCG CCGCAGCAGC CTTCGCCGGT TCTCGGCGCA
    GATGCCATTA CCCCGGCCTC CATTCAACTG CTGCAGCAGC TGCAGGCGGT GGGACTGCAG
    CACCAGTTGT TGCAAGCCCT CACAGGACTG GGAGCCCAGC AGAGCAGCTC GGCGACGGAC
    ACCAGCGCTG TGGCGGCCGC AGGACTTTTG ACCCCGATGA CCGTCCAGAA TCTAGCGGCC
    ATTGCGGCCA TGACGCAGCC GAGTCTCTCG AATGCCGCCG CGGCAGCCGC CGCTGCCACA
    TCGCCTGGAA GCGCCCAACT GACCAACACG GCTGCGCTGC TCTGGTCCGA TCCGAATCCA
    ATGGCATCGG CATATATGTC AACAGCCGCC GGTCTGCCGC AATTCGGGAG CGCCAGTGCA
    TTGTCCACTT CGCCGCTGGC CAGCGTTGCC CTCAGCGCCG CAGCCGCCGC CGCAGCGGGA
    AAGCAGATTG AAGGACCCGA GGGCTGCAAC CTTTTCATCT ACCATCTGCC GCAGGAGTTC
    ACCGATACGG ATCTGGCCTC CACGTTCCTG CCCTTCGGCA ATGTGATCTC GGCCAAGGTG
    TTCATCGACA AACAGACGAG CCTGTCGAAG TGCTTCGGGT TTGTCTCCTT CGACAATCCG
    GACTCGGCCC AGGTGGCCAT AAAGGCGATG AACGGCTTCC AGGTGGGCAC CAAGCGTCTC
    AAGGTGCAGC TGAAGAAGCC CAAGGACTCG AAGCCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260406.1
    CDS457..2178
    Translation

    Target ORF information:

    RefSeq Version NM_001273477.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGTGATA AGAGCAGCAG TGCAACGAAC AGCCTGCCGA ATAGTCCCAT ACACAGCAAC 
    AACAATAATC CATCGCTGCT GAACAACAAC AACAATAACA GCAACGGCAC CAGCAGCAAC
    AACAGCTTAA ACGTTGGCAA CAACAACAGC AATCCGAGCT TGGGCGGCAG CAACAGCAAC
    GCACTGGTTT CCGTAGGCAG CAACGGTATA ATGTCCGCCG CCGGTTTGGT CAACAACAAT
    AACAATCCGT GCAGCGCCAA CCGCAATGTG GTCGCCATGG TCGACGATGA CGCCTGCTTC
    CGTCTGGATA CGGACGCCAC GGTGACGTAC GGCGAGAAGG AACCGGATCC GGACAACATC
    AAGATGTTCG TCGGCCAGGT GCCCAAGTCG ATGGACGAGT CGCAGCTGCG CGAGATGTTC
    GAGGAGTACG GCGCCGTGCA CTCGATCAAC GTTCTGCGGG ACAAGGCTAC CGGAATTAGC
    AAGGGTTGCT GCTTTGTGAC ATTTTATACG AGACACGCCG CCCTGAAAGC CCAGGATGCT
    TTGCATAATG TTAAGACGCT AAATGGGATG TACCATCCCA TTCAAATGAA GCCCGCCGAT
    AGCGAGAATC GCAATGAACG CAAACTCTTT GTGGGTATGC TAAACAAGAA GCTGAACGAG
    AACGACGTTC GCAAGCTATT TGAAGTCCAC GGAGCCATCG AGGAGTGCAC CGTCCTTCGG
    GACCAAAACG GACAGAGCAA GGGTTGTGCC TTCGTCACAT TCGCCACCAA GCACGCTGCC
    ATTTCGGCCA TTAAAGTGAC CCTGAGCCAG AACAAGATCA TGGAGGGCTG CACATCGCCG
    CTGGTCGTCA AGTTCGCCGA TACGCAAAAA GAGAAGGAGC AGAAGAAGAT TCAGCAAATT
    CAGGCCAATC TCTGGAACCT GGCCAGCAAC ATCAACATAC CTCTGGGCCA AACGGCCACC
    AGCGTGACCA CGCCCATCCT GCCCAATCCG CCGCAGCAGC CTTCGCCGGT TCTCGGCGCA
    GATGCCATTA CCCCGGCCTC CATTCAACTG CTGCAGCAGC TGCAGGCGGT GGGACTGCAG
    CACCAGTTGT TGCAAGCCCT CACAGGACTG GGAGCCCAGC AGAGCAGCTC GGCGACGGAC
    ACCAGCGCTG TGGCGGCCGC AGGACTTTTG ACCCCGATGA CCGTCCAGAA TCTAGCGGCC
    ATTGCGGCCA TGACGCAGCC GAGTCTCTCG AATGCCGCCG CGGCAGCCGC CGCTGCCACA
    TCGCCTGGAA GCGCCCAACT GACCAACACG GCTGCGCTGC TCTGGTCCGA TCCGAATCCA
    ATGGCATCGG CATATATGTC AACAGCCGCC GGTCTGCCGC AATTCGGGAG CGCCAGTGCA
    TTGTCCACTT CGCCGCTGGC CAGCGTTGCC CTCAGCGCCG CAGCCGCCGC CGCAGCGGGA
    AAGCAGATTG AAGGACCCGA GGGCTGCAAC CTTTTCATCT ACCATCTGCC GCAGGAGTTC
    ACCGATACGG ATCTGGCCTC CACGTTCCTG CCCTTCGGCA ATGTGATCTC GGCCAAGGTG
    TTCATCGACA AACAGACGAG CCTGTCGAAG TGCTTCGGGT TTGTCTCCTT CGACAATCCG
    GACTCGGCCC AGGTGGCCAT AAAGGCGATG AACGGCTTCC AGGTGGGCAC CAAGCGTCTC
    AAGGTGCAGC TGAAGAAGCC CAAGGACTCG AAGCCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16707
    Clone ID Related Accession (Same CDS sequence) NM_001273476.1 , NM_001273477.1 , NM_001273478.1 , NM_001273475.1
    Accession Version NM_001273478.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGTGATA AGAGCAGCAG TGCAACGAAC AGCCTGCCGA ATAGTCCCAT ACACAGCAAC 
    AACAATAATC CATCGCTGCT GAACAACAAC AACAATAACA GCAACGGCAC CAGCAGCAAC
    AACAGCTTAA ACGTTGGCAA CAACAACAGC AATCCGAGCT TGGGCGGCAG CAACAGCAAC
    GCACTGGTTT CCGTAGGCAG CAACGGTATA ATGTCCGCCG CCGGTTTGGT CAACAACAAT
    AACAATCCGT GCAGCGCCAA CCGCAATGTG GTCGCCATGG TCGACGATGA CGCCTGCTTC
    CGTCTGGATA CGGACGCCAC GGTGACGTAC GGCGAGAAGG AACCGGATCC GGACAACATC
    AAGATGTTCG TCGGCCAGGT GCCCAAGTCG ATGGACGAGT CGCAGCTGCG CGAGATGTTC
    GAGGAGTACG GCGCCGTGCA CTCGATCAAC GTTCTGCGGG ACAAGGCTAC CGGAATTAGC
    AAGGGTTGCT GCTTTGTGAC ATTTTATACG AGACACGCCG CCCTGAAAGC CCAGGATGCT
    TTGCATAATG TTAAGACGCT AAATGGGATG TACCATCCCA TTCAAATGAA GCCCGCCGAT
    AGCGAGAATC GCAATGAACG CAAACTCTTT GTGGGTATGC TAAACAAGAA GCTGAACGAG
    AACGACGTTC GCAAGCTATT TGAAGTCCAC GGAGCCATCG AGGAGTGCAC CGTCCTTCGG
    GACCAAAACG GACAGAGCAA GGGTTGTGCC TTCGTCACAT TCGCCACCAA GCACGCTGCC
    ATTTCGGCCA TTAAAGTGAC CCTGAGCCAG AACAAGATCA TGGAGGGCTG CACATCGCCG
    CTGGTCGTCA AGTTCGCCGA TACGCAAAAA GAGAAGGAGC AGAAGAAGAT TCAGCAAATT
    CAGGCCAATC TCTGGAACCT GGCCAGCAAC ATCAACATAC CTCTGGGCCA AACGGCCACC
    AGCGTGACCA CGCCCATCCT GCCCAATCCG CCGCAGCAGC CTTCGCCGGT TCTCGGCGCA
    GATGCCATTA CCCCGGCCTC CATTCAACTG CTGCAGCAGC TGCAGGCGGT GGGACTGCAG
    CACCAGTTGT TGCAAGCCCT CACAGGACTG GGAGCCCAGC AGAGCAGCTC GGCGACGGAC
    ACCAGCGCTG TGGCGGCCGC AGGACTTTTG ACCCCGATGA CCGTCCAGAA TCTAGCGGCC
    ATTGCGGCCA TGACGCAGCC GAGTCTCTCG AATGCCGCCG CGGCAGCCGC CGCTGCCACA
    TCGCCTGGAA GCGCCCAACT GACCAACACG GCTGCGCTGC TCTGGTCCGA TCCGAATCCA
    ATGGCATCGG CATATATGTC AACAGCCGCC GGTCTGCCGC AATTCGGGAG CGCCAGTGCA
    TTGTCCACTT CGCCGCTGGC CAGCGTTGCC CTCAGCGCCG CAGCCGCCGC CGCAGCGGGA
    AAGCAGATTG AAGGACCCGA GGGCTGCAAC CTTTTCATCT ACCATCTGCC GCAGGAGTTC
    ACCGATACGG ATCTGGCCTC CACGTTCCTG CCCTTCGGCA ATGTGATCTC GGCCAAGGTG
    TTCATCGACA AACAGACGAG CCTGTCGAAG TGCTTCGGGT TTGTCTCCTT CGACAATCCG
    GACTCGGCCC AGGTGGCCAT AAAGGCGATG AACGGCTTCC AGGTGGGCAC CAAGCGTCTC
    AAGGTGCAGC TGAAGAAGCC CAAGGACTCG AAGCCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260407.1
    CDS428..2149
    Translation

    Target ORF information:

    RefSeq Version NM_001273478.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGTGATA AGAGCAGCAG TGCAACGAAC AGCCTGCCGA ATAGTCCCAT ACACAGCAAC 
    AACAATAATC CATCGCTGCT GAACAACAAC AACAATAACA GCAACGGCAC CAGCAGCAAC
    AACAGCTTAA ACGTTGGCAA CAACAACAGC AATCCGAGCT TGGGCGGCAG CAACAGCAAC
    GCACTGGTTT CCGTAGGCAG CAACGGTATA ATGTCCGCCG CCGGTTTGGT CAACAACAAT
    AACAATCCGT GCAGCGCCAA CCGCAATGTG GTCGCCATGG TCGACGATGA CGCCTGCTTC
    CGTCTGGATA CGGACGCCAC GGTGACGTAC GGCGAGAAGG AACCGGATCC GGACAACATC
    AAGATGTTCG TCGGCCAGGT GCCCAAGTCG ATGGACGAGT CGCAGCTGCG CGAGATGTTC
    GAGGAGTACG GCGCCGTGCA CTCGATCAAC GTTCTGCGGG ACAAGGCTAC CGGAATTAGC
    AAGGGTTGCT GCTTTGTGAC ATTTTATACG AGACACGCCG CCCTGAAAGC CCAGGATGCT
    TTGCATAATG TTAAGACGCT AAATGGGATG TACCATCCCA TTCAAATGAA GCCCGCCGAT
    AGCGAGAATC GCAATGAACG CAAACTCTTT GTGGGTATGC TAAACAAGAA GCTGAACGAG
    AACGACGTTC GCAAGCTATT TGAAGTCCAC GGAGCCATCG AGGAGTGCAC CGTCCTTCGG
    GACCAAAACG GACAGAGCAA GGGTTGTGCC TTCGTCACAT TCGCCACCAA GCACGCTGCC
    ATTTCGGCCA TTAAAGTGAC CCTGAGCCAG AACAAGATCA TGGAGGGCTG CACATCGCCG
    CTGGTCGTCA AGTTCGCCGA TACGCAAAAA GAGAAGGAGC AGAAGAAGAT TCAGCAAATT
    CAGGCCAATC TCTGGAACCT GGCCAGCAAC ATCAACATAC CTCTGGGCCA AACGGCCACC
    AGCGTGACCA CGCCCATCCT GCCCAATCCG CCGCAGCAGC CTTCGCCGGT TCTCGGCGCA
    GATGCCATTA CCCCGGCCTC CATTCAACTG CTGCAGCAGC TGCAGGCGGT GGGACTGCAG
    CACCAGTTGT TGCAAGCCCT CACAGGACTG GGAGCCCAGC AGAGCAGCTC GGCGACGGAC
    ACCAGCGCTG TGGCGGCCGC AGGACTTTTG ACCCCGATGA CCGTCCAGAA TCTAGCGGCC
    ATTGCGGCCA TGACGCAGCC GAGTCTCTCG AATGCCGCCG CGGCAGCCGC CGCTGCCACA
    TCGCCTGGAA GCGCCCAACT GACCAACACG GCTGCGCTGC TCTGGTCCGA TCCGAATCCA
    ATGGCATCGG CATATATGTC AACAGCCGCC GGTCTGCCGC AATTCGGGAG CGCCAGTGCA
    TTGTCCACTT CGCCGCTGGC CAGCGTTGCC CTCAGCGCCG CAGCCGCCGC CGCAGCGGGA
    AAGCAGATTG AAGGACCCGA GGGCTGCAAC CTTTTCATCT ACCATCTGCC GCAGGAGTTC
    ACCGATACGG ATCTGGCCTC CACGTTCCTG CCCTTCGGCA ATGTGATCTC GGCCAAGGTG
    TTCATCGACA AACAGACGAG CCTGTCGAAG TGCTTCGGGT TTGTCTCCTT CGACAATCCG
    GACTCGGCCC AGGTGGCCAT AAAGGCGATG AACGGCTTCC AGGTGGGCAC CAAGCGTCTC
    AAGGTGCAGC TGAAGAAGCC CAAGGACTCG AAGCCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16707
    Clone ID Related Accession (Same CDS sequence) NM_001273476.1 , NM_001273477.1 , NM_001273478.1 , NM_001273475.1
    Accession Version NM_001273475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 1, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGTGATA AGAGCAGCAG TGCAACGAAC AGCCTGCCGA ATAGTCCCAT ACACAGCAAC 
    AACAATAATC CATCGCTGCT GAACAACAAC AACAATAACA GCAACGGCAC CAGCAGCAAC
    AACAGCTTAA ACGTTGGCAA CAACAACAGC AATCCGAGCT TGGGCGGCAG CAACAGCAAC
    GCACTGGTTT CCGTAGGCAG CAACGGTATA ATGTCCGCCG CCGGTTTGGT CAACAACAAT
    AACAATCCGT GCAGCGCCAA CCGCAATGTG GTCGCCATGG TCGACGATGA CGCCTGCTTC
    CGTCTGGATA CGGACGCCAC GGTGACGTAC GGCGAGAAGG AACCGGATCC GGACAACATC
    AAGATGTTCG TCGGCCAGGT GCCCAAGTCG ATGGACGAGT CGCAGCTGCG CGAGATGTTC
    GAGGAGTACG GCGCCGTGCA CTCGATCAAC GTTCTGCGGG ACAAGGCTAC CGGAATTAGC
    AAGGGTTGCT GCTTTGTGAC ATTTTATACG AGACACGCCG CCCTGAAAGC CCAGGATGCT
    TTGCATAATG TTAAGACGCT AAATGGGATG TACCATCCCA TTCAAATGAA GCCCGCCGAT
    AGCGAGAATC GCAATGAACG CAAACTCTTT GTGGGTATGC TAAACAAGAA GCTGAACGAG
    AACGACGTTC GCAAGCTATT TGAAGTCCAC GGAGCCATCG AGGAGTGCAC CGTCCTTCGG
    GACCAAAACG GACAGAGCAA GGGTTGTGCC TTCGTCACAT TCGCCACCAA GCACGCTGCC
    ATTTCGGCCA TTAAAGTGAC CCTGAGCCAG AACAAGATCA TGGAGGGCTG CACATCGCCG
    CTGGTCGTCA AGTTCGCCGA TACGCAAAAA GAGAAGGAGC AGAAGAAGAT TCAGCAAATT
    CAGGCCAATC TCTGGAACCT GGCCAGCAAC ATCAACATAC CTCTGGGCCA AACGGCCACC
    AGCGTGACCA CGCCCATCCT GCCCAATCCG CCGCAGCAGC CTTCGCCGGT TCTCGGCGCA
    GATGCCATTA CCCCGGCCTC CATTCAACTG CTGCAGCAGC TGCAGGCGGT GGGACTGCAG
    CACCAGTTGT TGCAAGCCCT CACAGGACTG GGAGCCCAGC AGAGCAGCTC GGCGACGGAC
    ACCAGCGCTG TGGCGGCCGC AGGACTTTTG ACCCCGATGA CCGTCCAGAA TCTAGCGGCC
    ATTGCGGCCA TGACGCAGCC GAGTCTCTCG AATGCCGCCG CGGCAGCCGC CGCTGCCACA
    TCGCCTGGAA GCGCCCAACT GACCAACACG GCTGCGCTGC TCTGGTCCGA TCCGAATCCA
    ATGGCATCGG CATATATGTC AACAGCCGCC GGTCTGCCGC AATTCGGGAG CGCCAGTGCA
    TTGTCCACTT CGCCGCTGGC CAGCGTTGCC CTCAGCGCCG CAGCCGCCGC CGCAGCGGGA
    AAGCAGATTG AAGGACCCGA GGGCTGCAAC CTTTTCATCT ACCATCTGCC GCAGGAGTTC
    ACCGATACGG ATCTGGCCTC CACGTTCCTG CCCTTCGGCA ATGTGATCTC GGCCAAGGTG
    TTCATCGACA AACAGACGAG CCTGTCGAAG TGCTTCGGGT TTGTCTCCTT CGACAATCCG
    GACTCGGCCC AGGTGGCCAT AAAGGCGATG AACGGCTTCC AGGTGGGCAC CAAGCGTCTC
    AAGGTGCAGC TGAAGAAGCC CAAGGACTCG AAGCCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260404.1
    CDS299..2020
    Translation

    Target ORF information:

    RefSeq Version NM_001273475.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 1 (bru1), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGTGATA AGAGCAGCAG TGCAACGAAC AGCCTGCCGA ATAGTCCCAT ACACAGCAAC 
    AACAATAATC CATCGCTGCT GAACAACAAC AACAATAACA GCAACGGCAC CAGCAGCAAC
    AACAGCTTAA ACGTTGGCAA CAACAACAGC AATCCGAGCT TGGGCGGCAG CAACAGCAAC
    GCACTGGTTT CCGTAGGCAG CAACGGTATA ATGTCCGCCG CCGGTTTGGT CAACAACAAT
    AACAATCCGT GCAGCGCCAA CCGCAATGTG GTCGCCATGG TCGACGATGA CGCCTGCTTC
    CGTCTGGATA CGGACGCCAC GGTGACGTAC GGCGAGAAGG AACCGGATCC GGACAACATC
    AAGATGTTCG TCGGCCAGGT GCCCAAGTCG ATGGACGAGT CGCAGCTGCG CGAGATGTTC
    GAGGAGTACG GCGCCGTGCA CTCGATCAAC GTTCTGCGGG ACAAGGCTAC CGGAATTAGC
    AAGGGTTGCT GCTTTGTGAC ATTTTATACG AGACACGCCG CCCTGAAAGC CCAGGATGCT
    TTGCATAATG TTAAGACGCT AAATGGGATG TACCATCCCA TTCAAATGAA GCCCGCCGAT
    AGCGAGAATC GCAATGAACG CAAACTCTTT GTGGGTATGC TAAACAAGAA GCTGAACGAG
    AACGACGTTC GCAAGCTATT TGAAGTCCAC GGAGCCATCG AGGAGTGCAC CGTCCTTCGG
    GACCAAAACG GACAGAGCAA GGGTTGTGCC TTCGTCACAT TCGCCACCAA GCACGCTGCC
    ATTTCGGCCA TTAAAGTGAC CCTGAGCCAG AACAAGATCA TGGAGGGCTG CACATCGCCG
    CTGGTCGTCA AGTTCGCCGA TACGCAAAAA GAGAAGGAGC AGAAGAAGAT TCAGCAAATT
    CAGGCCAATC TCTGGAACCT GGCCAGCAAC ATCAACATAC CTCTGGGCCA AACGGCCACC
    AGCGTGACCA CGCCCATCCT GCCCAATCCG CCGCAGCAGC CTTCGCCGGT TCTCGGCGCA
    GATGCCATTA CCCCGGCCTC CATTCAACTG CTGCAGCAGC TGCAGGCGGT GGGACTGCAG
    CACCAGTTGT TGCAAGCCCT CACAGGACTG GGAGCCCAGC AGAGCAGCTC GGCGACGGAC
    ACCAGCGCTG TGGCGGCCGC AGGACTTTTG ACCCCGATGA CCGTCCAGAA TCTAGCGGCC
    ATTGCGGCCA TGACGCAGCC GAGTCTCTCG AATGCCGCCG CGGCAGCCGC CGCTGCCACA
    TCGCCTGGAA GCGCCCAACT GACCAACACG GCTGCGCTGC TCTGGTCCGA TCCGAATCCA
    ATGGCATCGG CATATATGTC AACAGCCGCC GGTCTGCCGC AATTCGGGAG CGCCAGTGCA
    TTGTCCACTT CGCCGCTGGC CAGCGTTGCC CTCAGCGCCG CAGCCGCCGC CGCAGCGGGA
    AAGCAGATTG AAGGACCCGA GGGCTGCAAC CTTTTCATCT ACCATCTGCC GCAGGAGTTC
    ACCGATACGG ATCTGGCCTC CACGTTCCTG CCCTTCGGCA ATGTGATCTC GGCCAAGGTG
    TTCATCGACA AACAGACGAG CCTGTCGAAG TGCTTCGGGT TTGTCTCCTT CGACAATCCG
    GACTCGGCCC AGGTGGCCAT AAAGGCGATG AACGGCTTCC AGGTGGGCAC CAAGCGTCTC
    AAGGTGCAGC TGAAGAAGCC CAAGGACTCG AAGCCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.