crol cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following crol gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crol cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa14467 NM_164971.2
Latest version!
Drosophila melanogaster crooked legs (crol), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa14468 NM_057896.4
Latest version!
Drosophila melanogaster crooked legs (crol), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa14469 NM_057897.4
Latest version!
Drosophila melanogaster crooked legs (crol), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa14470 NM_001259064.2
Latest version!
Drosophila melanogaster crooked legs (crol), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa14470 NM_057895.4
Latest version!
Drosophila melanogaster crooked legs (crol), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa14470 NM_001273464.1
Latest version!
Drosophila melanogaster crooked legs (crol), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa16702 NM_001273462.1
Latest version!
Drosophila melanogaster crooked legs (crol), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa16704 NM_001273461.1
Latest version!
Drosophila melanogaster crooked legs (crol), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa16705 NM_001273463.1
Latest version!
Drosophila melanogaster crooked legs (crol), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa14467
    Clone ID Related Accession (Same CDS sequence) NM_164971.2
    Accession Version NM_164971.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crooked legs, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164971.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGCGAGAC GCCGCACCGG TGCGACTTTT GCTCCAAGAC GTTTACGCGA
    AAGGAGCACT TGCTTAACCA CGTGCGCCAG CATACGGGAG AGTCGCCACA CCGCTGCTCC
    TACTGCATGA AGACGTTCAC GCGCAAGGAG CACCTGGTCA ACCACATACG CCAGCACACG
    GGCGAGTCGC CGCACAAGTG CACGTACTGC ACCAAGACGT TCACGCGCAA GGAGCACCTG
    ACGAACCATG TGCGCCAGCA CACGGGCGAC TCCCCGCACC GCTGCTCCTA CTGCAAGAAG
    ACCTTCACGC GCAAGGAGCA CCTGACGAAC CATGTGCGCC TGCACACGGG CGACTCGCCG
    CACAAGTGCG AGTACTGCCA GAAGACGTTT ACGCGGAAGG AGCACCTCAA CAATCATATG
    CGCCAGCATT CGAGCGACAA TCCGCATTGC TGCAACGTTT GCAACAAGCC GTTTACGCGC
    AAGGAACACC TGATCAACCA TATGTCGCGG TGCCACACCG GTGACCGGCC CTTCACCTGC
    GAGACGTGCG GCAAATCGTT CCCGCTCAAG GGCAATCTGC TCTTCCATCA GCGCAGCCAT
    ACCAAGGGCC AGGAGATGGA GCGGCCATTC GCCTGCGAGA AGTGCCCCAA GAACTTCATC
    TGCAAAGGTC ACTTGGTCTC GCACATGCGC TCCCATTCGG GTGAGAAACC ACACGCGTGC
    ACACTGTGCA GCAAGGCGTT CGTCGAGCGC GGCAATTTGA AGCGCCACAT GAAGATGAAT
    CACCCGGATG CTATGATGCC GCCACCACCC GTGCATCCGC ATCCGCAAAT ACCGGCTGGT
    GTGCTGACGC AAGTCAAGCA GGAAGTGAAA CCGATCATAA TTCCCCATCA CTCGGCGACC
    ACCACAATGC ACACCATTCA GCAGATCACG GCGGGTGCGG CGGGCGGAGC CGGTGCGGTC
    CAGTTAACAC CGGGTCTGGT GCCCCTGGTT ACCTCTACGC TCATCTCACA TAATGCGGCT
    GCTCAACAGC AGTCGCAGAA GCAGCAAGCA GCCGCCGCAG CAGCTGCACA GCAACAGGCC
    GCAGCCGCCG CTGCTGCCCA ACAGCAAGCA GCTCAGCAAC AGGCGGCTGC TGCACATCAG
    CAGCATCAAC AACAAGTGGC CGCGCAACAT CAACAGCAGG CTGCAGTGGC TGCACACCAG
    CAGCAGCAAC AGCAGTTGCA GCAGCAGCAA CAACTGCTTC AGTTGTCCAT CCAACAGGCG
    GCTCACCATC ATCAGCAGGA GCAGCATCGT CAACAGCAGC AACAGCAACA CCAGCAGCAA
    CAACAGCAAC AGCATCACCA GCAGCAACAG CAGGGTCATC CACAGGCCCC GCCACCGCAG
    CAGCAACAGC AGCCGCCGCC CATCGCCTTG ATCAGTGACC CAAGTGCTCT GGCACGTGCC
    GCCATCCAGC TGCAGCATCT GCCAGCGAAC GTGGAACAGC ACCCGGTTGT TTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723687.1
    CDS773..3409
    Translation

    Target ORF information:

    RefSeq Version NM_164971.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crooked legs (crol), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164971.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGCGAGAC GCCGCACCGG TGCGACTTTT GCTCCAAGAC GTTTACGCGA
    AAGGAGCACT TGCTTAACCA CGTGCGCCAG CATACGGGAG AGTCGCCACA CCGCTGCTCC
    TACTGCATGA AGACGTTCAC GCGCAAGGAG CACCTGGTCA ACCACATACG CCAGCACACG
    GGCGAGTCGC CGCACAAGTG CACGTACTGC ACCAAGACGT TCACGCGCAA GGAGCACCTG
    ACGAACCATG TGCGCCAGCA CACGGGCGAC TCCCCGCACC GCTGCTCCTA CTGCAAGAAG
    ACCTTCACGC GCAAGGAGCA CCTGACGAAC CATGTGCGCC TGCACACGGG CGACTCGCCG
    CACAAGTGCG AGTACTGCCA GAAGACGTTT ACGCGGAAGG AGCACCTCAA CAATCATATG
    CGCCAGCATT CGAGCGACAA TCCGCATTGC TGCAACGTTT GCAACAAGCC GTTTACGCGC
    AAGGAACACC TGATCAACCA TATGTCGCGG TGCCACACCG GTGACCGGCC CTTCACCTGC
    GAGACGTGCG GCAAATCGTT CCCGCTCAAG GGCAATCTGC TCTTCCATCA GCGCAGCCAT
    ACCAAGGGCC AGGAGATGGA GCGGCCATTC GCCTGCGAGA AGTGCCCCAA GAACTTCATC
    TGCAAAGGTC ACTTGGTCTC GCACATGCGC TCCCATTCGG GTGAGAAACC ACACGCGTGC
    ACACTGTGCA GCAAGGCGTT CGTCGAGCGC GGCAATTTGA AGCGCCACAT GAAGATGAAT
    CACCCGGATG CTATGATGCC GCCACCACCC GTGCATCCGC ATCCGCAAAT ACCGGCTGGT
    GTGCTGACGC AAGTCAAGCA GGAAGTGAAA CCGATCATAA TTCCCCATCA CTCGGCGACC
    ACCACAATGC ACACCATTCA GCAGATCACG GCGGGTGCGG CGGGCGGAGC CGGTGCGGTC
    CAGTTAACAC CGGGTCTGGT GCCCCTGGTT ACCTCTACGC TCATCTCACA TAATGCGGCT
    GCTCAACAGC AGTCGCAGAA GCAGCAAGCA GCCGCCGCAG CAGCTGCACA GCAACAGGCC
    GCAGCCGCCG CTGCTGCCCA ACAGCAAGCA GCTCAGCAAC AGGCGGCTGC TGCACATCAG
    CAGCATCAAC AACAAGTGGC CGCGCAACAT CAACAGCAGG CTGCAGTGGC TGCACACCAG
    CAGCAGCAAC AGCAGTTGCA GCAGCAGCAA CAACTGCTTC AGTTGTCCAT CCAACAGGCG
    GCTCACCATC ATCAGCAGGA GCAGCATCGT CAACAGCAGC AACAGCAACA CCAGCAGCAA
    CAACAGCAAC AGCATCACCA GCAGCAACAG CAGGGTCATC CACAGGCCCC GCCACCGCAG
    CAGCAACAGC AGCCGCCGCC CATCGCCTTG ATCAGTGACC CAAGTGCTCT GGCACGTGCC
    GCCATCCAGC TGCAGCATCT GCCAGCGAAC GTGGAACAGC ACCCGGTTGT TTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14468
    Clone ID Related Accession (Same CDS sequence) NM_057896.4
    Accession Version NM_057896.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crooked legs, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057896.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTCAA
    ACGCCGCACC AGTGCGATGT CTGCGGCAAG AAATATACGC GCAAGGAGCA CCTAGCCAAT
    CATATGCGAT CCCATACCAA CGAGACGCCG TTCCGTTGCG AGATCTGCGG CAAGAGCTTT
    AGCCGCAAGG AGCACTTCAC CAATCACATA CTTTGGCATA CAGGCGAGAC GCCGCACCGG
    TGCGACTTTT GCTCCAAGAC GTTTACGCGA AAGGAGCACT TGCTTAACCA CGTGCGCCAG
    CATACGGGAG AGTCGCCACA CCGCTGCTCC TACTGCATGA AGACGTTCAC GCGCAAGGAG
    CACCTGGTCA ACCACATACG CCAGCACACG GGTGAGACAC CGTTCAAGTG CACGTACTGC
    ACGAAAGCGT TCACGCGCAA AGATCACATG GTTAATCATG TACGGCAACA TACAGGCGAG
    TCGCCGCACA AGTGCACGTA CTGCACCAAG ACGTTCACGC GCAAGGAGCA CCTGACGAAC
    CATGTGCGCC AGCACACGGG CGACTCCCCG CACCGCTGCT CCTACTGCAA GAAGACCTTC
    ACGCGCAAGG AGCACCTGAC GAACCATGTG CGCCTGCACA CGGGCGACTC GCCGCACAAG
    TGCGAGTACT GCCAGAAGAC GTTTACGCGG AAGGAGCACC TCAACAATCA TATGCGCCAG
    CATTCGAGCG ACAATCCGCA TTGCTGCAAC GTTTGCAACA AGCCGTTTAC GCGCAAGGAA
    CACCTGATCA ACCATATGTC GCGGTGCCAC ACCGGTGACC GGCCCTTCAC CTGCGAGACG
    TGCGGCAAAT CGTTCCCGCT CAAGGGCAAT CTGCTCTTCC ATCAGCGCAG CCATACCAAG
    GGCCAGGAGA TGGAGCGGCC ATTCGCCTGC GAGAAGTGCC CCAAGAACTT CATCTGCAAA
    GGTCACTTGG TCTCGCACAT GCGCTCCCAT TCGGGTGAGA AACCACACGC GTGCACACTG
    TGCAGCAAGG CGTTCGTCGA GCGCGGCAAT TTGAAGCGCC ACATGAAGAT GAATCACCCG
    GATGCTATGA TGCCGCCACC ACCCGTGCAT CCGCATCCGC AAATACCGGC TGGTGTGCTG
    ACGCAAGTCA AGCAGGAAGT GAAACCGATC ATAATTCCCC ATCACTCGGC GACCACCACA
    ATGCACACCA TTCAGCAGAT CACGGCGGGT GCGGCGGGCG GAGCCGGTGC GGTCCAGTTA
    ACACCGGGTC TGGTGCCCCT GGTTACCTCT ACGCTCATCT CACATAATGC GGCTGCTCAA
    CAGCAGTCGC AGAAGCAGCA AGCAGCCGCC GCAGCAGCTG CACAGCAACA GGCCGCAGCC
    GCCGCTGCTG CCCAACAGCA AGCAGCTCAG CAACAGGCGG CTGCTGCACA TCAGCAGCAT
    CAACAACAAG TGGCCGCGCA ACATCAACAG CAGGCTGCAG TGGCTGCACA CCAGCAGCAG
    CAACAGCAGT TGCAGCAGCA GCAACAACTG CTTCAGTTGT CCATCCAACA GGCGGCTCAC
    CATCATCAGC AGGAGCAGCA TCGTCAACAG CAGCAACAGC AACACCAGCA GCAACAACAG
    CAACAGCATC ACCAGCAGCA ACAGCAGGGT CATCCACAGG CCCCGCCACC GCAGCAGCAA
    CAGCAGCCGC CGCCCATCGC CTTGATCAGT GACCCAAGTG CTCTGGCACG TGCCGCCATC
    CAGCTGCAGC ATCTGCCAGC GAACGTGGAA CAGCACCCGG TTGTTTACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477244.1
    CDS1419..3689
    Translation

    Target ORF information:

    RefSeq Version NM_057896.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crooked legs (crol), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057896.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTCAA
    ACGCCGCACC AGTGCGATGT CTGCGGCAAG AAATATACGC GCAAGGAGCA CCTAGCCAAT
    CATATGCGAT CCCATACCAA CGAGACGCCG TTCCGTTGCG AGATCTGCGG CAAGAGCTTT
    AGCCGCAAGG AGCACTTCAC CAATCACATA CTTTGGCATA CAGGCGAGAC GCCGCACCGG
    TGCGACTTTT GCTCCAAGAC GTTTACGCGA AAGGAGCACT TGCTTAACCA CGTGCGCCAG
    CATACGGGAG AGTCGCCACA CCGCTGCTCC TACTGCATGA AGACGTTCAC GCGCAAGGAG
    CACCTGGTCA ACCACATACG CCAGCACACG GGTGAGACAC CGTTCAAGTG CACGTACTGC
    ACGAAAGCGT TCACGCGCAA AGATCACATG GTTAATCATG TACGGCAACA TACAGGCGAG
    TCGCCGCACA AGTGCACGTA CTGCACCAAG ACGTTCACGC GCAAGGAGCA CCTGACGAAC
    CATGTGCGCC AGCACACGGG CGACTCCCCG CACCGCTGCT CCTACTGCAA GAAGACCTTC
    ACGCGCAAGG AGCACCTGAC GAACCATGTG CGCCTGCACA CGGGCGACTC GCCGCACAAG
    TGCGAGTACT GCCAGAAGAC GTTTACGCGG AAGGAGCACC TCAACAATCA TATGCGCCAG
    CATTCGAGCG ACAATCCGCA TTGCTGCAAC GTTTGCAACA AGCCGTTTAC GCGCAAGGAA
    CACCTGATCA ACCATATGTC GCGGTGCCAC ACCGGTGACC GGCCCTTCAC CTGCGAGACG
    TGCGGCAAAT CGTTCCCGCT CAAGGGCAAT CTGCTCTTCC ATCAGCGCAG CCATACCAAG
    GGCCAGGAGA TGGAGCGGCC ATTCGCCTGC GAGAAGTGCC CCAAGAACTT CATCTGCAAA
    GGTCACTTGG TCTCGCACAT GCGCTCCCAT TCGGGTGAGA AACCACACGC GTGCACACTG
    TGCAGCAAGG CGTTCGTCGA GCGCGGCAAT TTGAAGCGCC ACATGAAGAT GAATCACCCG
    GATGCTATGA TGCCGCCACC ACCCGTGCAT CCGCATCCGC AAATACCGGC TGGTGTGCTG
    ACGCAAGTCA AGCAGGAAGT GAAACCGATC ATAATTCCCC ATCACTCGGC GACCACCACA
    ATGCACACCA TTCAGCAGAT CACGGCGGGT GCGGCGGGCG GAGCCGGTGC GGTCCAGTTA
    ACACCGGGTC TGGTGCCCCT GGTTACCTCT ACGCTCATCT CACATAATGC GGCTGCTCAA
    CAGCAGTCGC AGAAGCAGCA AGCAGCCGCC GCAGCAGCTG CACAGCAACA GGCCGCAGCC
    GCCGCTGCTG CCCAACAGCA AGCAGCTCAG CAACAGGCGG CTGCTGCACA TCAGCAGCAT
    CAACAACAAG TGGCCGCGCA ACATCAACAG CAGGCTGCAG TGGCTGCACA CCAGCAGCAG
    CAACAGCAGT TGCAGCAGCA GCAACAACTG CTTCAGTTGT CCATCCAACA GGCGGCTCAC
    CATCATCAGC AGGAGCAGCA TCGTCAACAG CAGCAACAGC AACACCAGCA GCAACAACAG
    CAACAGCATC ACCAGCAGCA ACAGCAGGGT CATCCACAGG CCCCGCCACC GCAGCAGCAA
    CAGCAGCCGC CGCCCATCGC CTTGATCAGT GACCCAAGTG CTCTGGCACG TGCCGCCATC
    CAGCTGCAGC ATCTGCCAGC GAACGTGGAA CAGCACCCGG TTGTTTACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14469
    Clone ID Related Accession (Same CDS sequence) NM_057897.4
    Accession Version NM_057897.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crooked legs, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057897.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGTCAAAC GCCGCACCAG TGCGATGTCT GCGGCAAGAA ATATACGCGC
    AAGGAGCACC TAGCCAATCA TATGCGATCC CATACCAACG AGACGCCGTT CCGTTGCGAG
    ATCTGCGGCA AGAGCTTTAG CCGCAAGGAG CACTTCACCA ATCACATACT TTGGCATACA
    GGCGAGACGC CGCACCGGTG CGACTTTTGC TCCAAGACGT TTACGCGAAA GGAGCACTTG
    CTTAACCACG TGCGCCAGCA TACGGGAGAG TCGCCACACC GCTGCTCCTA CTGCATGAAG
    ACGTTCACGC GCAAGGAGCA CCTGGTCAAC CACATACGCC AGCACACGGG TGAGACACCG
    TTCAAGTGCA CGTACTGCAC GAAAGCGTTC ACGCGCAAAG ATCACATGGT TAATCATGTA
    CGGCAACATA CAGGCGAGTC GCCGCACAAG TGCACGTACT GCACCAAGAC GTTCACGCGC
    AAGGAGCACC TGACGAACCA TGTGCGCCAG CACACGGGCG ACTCCCCGCA CCGCTGCTCC
    TACTGCAAGA AGACCTTCAC GCGCAAGGAG CACCTGACGA ACCATGTGCG CCTGCACACG
    GGCGACTCGC CGCACAAGTG CGAGTACTGC CAGAAGACGT TTACGCGGAA GGAGCACCTC
    AACAATCATA TGCGCCAGCA TTCGAGCGAC AATCCGCATT GCTGCAACGT TTGCAACAAG
    CCGTTTACGC GCAAGGAACA CCTGATCAAC CATATGTCGC GGTGCCACAC CGGTGACCGG
    CCCTTCACCT GCGAGACGTG CGGCAAATCG TTCCCGCTCA AGGGCAATCT GCTCTTCCAT
    CAGCGCAGCC ATACCAAGGG CCAGGAGATG GAGCGGCCAT TCGCCTGCGA GAAGTGCCCC
    AAGAACTTCA TCTGCAAAGT TCCCCATCAC TCGGCGACCA CCACAATGCA CACCATTCAG
    CAGATCACGG CGGGTGCGGC GGGCGGAGCC GGTGCGGTCC AGTTAACACC GGGTCTGGTG
    CCCCTGGTTA CCTCTACGCT CATCTCACAT AATGCGGCTG CTCAACAGCA GTCGCAGAAG
    CAGCAAGCAG CCGCCGCAGC AGCTGCACAG CAACAGGCCG CAGCCGCCGC TGCTGCCCAA
    CAGCAAGCAG CTCAGCAACA GGCGGCTGCT GCACATCAGC AGCATCAACA ACAAGTGGCC
    GCGCAACATC AACAGCAGGC TGCAGTGGCT GCACACCAGC AGCAGCAACA GCAGTTGCAG
    CAGCAGCAAC AACTGCTTCA GTTGTCCATC CAACAGGCGG CTCACCATCA TCAGCAGGAG
    CAGCATCGTC AACAGCAGCA ACAGCAACAC CAGCAGCAAC AACAGCAACA GCATCACCAG
    CAGCAACAGC AGGGTCATCC ACAGGCCCCG CCACCGCAGC AGCAACAGCA GCCGCCGCCC
    ATCGCCTTGA TCAGTGACCC AAGTGCTCTG GCACGTGCCG CCATCCAGCT GCAGCATCTG
    CCAGCGAACG TGGAACAGCA CCCGGTTGTT TACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477245.1
    CDS1419..4094
    Translation

    Target ORF information:

    RefSeq Version NM_057897.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crooked legs (crol), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057897.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGTCAAAC GCCGCACCAG TGCGATGTCT GCGGCAAGAA ATATACGCGC
    AAGGAGCACC TAGCCAATCA TATGCGATCC CATACCAACG AGACGCCGTT CCGTTGCGAG
    ATCTGCGGCA AGAGCTTTAG CCGCAAGGAG CACTTCACCA ATCACATACT TTGGCATACA
    GGCGAGACGC CGCACCGGTG CGACTTTTGC TCCAAGACGT TTACGCGAAA GGAGCACTTG
    CTTAACCACG TGCGCCAGCA TACGGGAGAG TCGCCACACC GCTGCTCCTA CTGCATGAAG
    ACGTTCACGC GCAAGGAGCA CCTGGTCAAC CACATACGCC AGCACACGGG TGAGACACCG
    TTCAAGTGCA CGTACTGCAC GAAAGCGTTC ACGCGCAAAG ATCACATGGT TAATCATGTA
    CGGCAACATA CAGGCGAGTC GCCGCACAAG TGCACGTACT GCACCAAGAC GTTCACGCGC
    AAGGAGCACC TGACGAACCA TGTGCGCCAG CACACGGGCG ACTCCCCGCA CCGCTGCTCC
    TACTGCAAGA AGACCTTCAC GCGCAAGGAG CACCTGACGA ACCATGTGCG CCTGCACACG
    GGCGACTCGC CGCACAAGTG CGAGTACTGC CAGAAGACGT TTACGCGGAA GGAGCACCTC
    AACAATCATA TGCGCCAGCA TTCGAGCGAC AATCCGCATT GCTGCAACGT TTGCAACAAG
    CCGTTTACGC GCAAGGAACA CCTGATCAAC CATATGTCGC GGTGCCACAC CGGTGACCGG
    CCCTTCACCT GCGAGACGTG CGGCAAATCG TTCCCGCTCA AGGGCAATCT GCTCTTCCAT
    CAGCGCAGCC ATACCAAGGG CCAGGAGATG GAGCGGCCAT TCGCCTGCGA GAAGTGCCCC
    AAGAACTTCA TCTGCAAAGT TCCCCATCAC TCGGCGACCA CCACAATGCA CACCATTCAG
    CAGATCACGG CGGGTGCGGC GGGCGGAGCC GGTGCGGTCC AGTTAACACC GGGTCTGGTG
    CCCCTGGTTA CCTCTACGCT CATCTCACAT AATGCGGCTG CTCAACAGCA GTCGCAGAAG
    CAGCAAGCAG CCGCCGCAGC AGCTGCACAG CAACAGGCCG CAGCCGCCGC TGCTGCCCAA
    CAGCAAGCAG CTCAGCAACA GGCGGCTGCT GCACATCAGC AGCATCAACA ACAAGTGGCC
    GCGCAACATC AACAGCAGGC TGCAGTGGCT GCACACCAGC AGCAGCAACA GCAGTTGCAG
    CAGCAGCAAC AACTGCTTCA GTTGTCCATC CAACAGGCGG CTCACCATCA TCAGCAGGAG
    CAGCATCGTC AACAGCAGCA ACAGCAACAC CAGCAGCAAC AACAGCAACA GCATCACCAG
    CAGCAACAGC AGGGTCATCC ACAGGCCCCG CCACCGCAGC AGCAACAGCA GCCGCCGCCC
    ATCGCCTTGA TCAGTGACCC AAGTGCTCTG GCACGTGCCG CCATCCAGCT GCAGCATCTG
    CCAGCGAACG TGGAACAGCA CCCGGTTGTT TACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14470
    Clone ID Related Accession (Same CDS sequence) NM_001259064.2 , NM_001273464.1 , NM_057895.4
    Accession Version NM_001259064.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crooked legs, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259064.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGTCAAAC GCCGCACCAG TGCGATGTCT GCGGCAAGAA ATATACGCGC
    AAGGAGCACC TAGCCAATCA TATGCGATCC CATACCAACG AGACGCCGTT CCGTTGCGAG
    ATCTGCGGCA AGAGCTTTAG CCGCAAGGAG CACTTCACCA ATCACATACT TTGGCATACA
    GGCGAGACGC CGCACCGGTG CGACTTTTGC TCCAAGACGT TTACGCGAAA GGAGCACTTG
    CTTAACCACG TGCGCCAGCA TACGGGAGAG TCGCCACACC GCTGCTCCTA CTGCATGAAG
    ACGTTCACGC GCAAGGAGCA CCTGGTCAAC CACATACGCC AGCACACGGG TGAGACACCG
    TTCAAGTGCA CGTACTGCAC GAAAGCGTTC ACGCGCAAAG ATCACATGGT TAATCATGTA
    CGGCAACATA CAGGCGAGTC GCCGCACAAG TGCACGTACT GCACCAAGAC GTTCACGCGC
    AAGGAGCACC TGACGAACCA TGTGCGCCAG CACACGGGCG ACTCCCCGCA CCGCTGCTCC
    TACTGCAAGA AGACCTTCAC GCGCAAGGAG CACCTGACGA ACCATGTGCG CCTGCACACG
    GGCGACTCGC CGCACAAGTG CGAGTACTGC CAGAAGACGT TTACGCGGAA GGAGCACCTC
    AACAATCATA TGCGCCAGCA TTCGAGCGAC AATCCGCATT GCTGCAACGT TTGCAACAAG
    CCGTTTACGC GCAAGGAACA CCTGATCAAC CATATGTCGC GGTGCCACAC CGGTGACCGG
    CCCTTCACCT GCGAGACGTG CGGCAAATCG TTCCCGCTCA AGGGCAATCT GCTCTTCCAT
    CAGCGCAGCC ATACCAAGGG CCAGGAGATG GAGCGGCCAT TCGCCTGCGA GAAGTGCCCC
    AAGAACTTCA TCTGCAAAGG TCACTTGGTC TCGCACATGC GCTCCCATTC GGGTGAGAAA
    CCACACGCGT GCACACTGTG CAGCAAGGCG TTCGTCGAGC GCGGCAATTT GAAGCGCCAC
    ATGAAGATGA ATCACCCGGA TGCTATGATG CCGCCACCAC CCGTGCATCC GCATCCGCAA
    ATACCGGCTG GTGTGCTGAC GCAAGTCAAG CAGGAAGTGA AACCGATCAT AATTCCCCAT
    CACTCGGCGA CCACCACAAT GCACACCATT CAGCAGATCA CGGCGGGTGC GGCGGGCGGA
    GCCGGTGCGG TCCAGTTAAC ACCGGGTCTG GTGCCCCTGG TTACCTCTAC GCTCATCTCA
    CATAATGCGG CTGCTCAACA GCAGTCGCAG AAGCAGCAAG CAGCCGCCGC AGCAGCTGCA
    CAGCAACAGG CCGCAGCCGC CGCTGCTGCC CAACAGCAAG CAGCTCAGCA ACAGGCGGCT
    GCTGCACATC AGCAGCATCA ACAACAAGTG GCCGCGCAAC ATCAACAGCA GGCTGCAGTG
    GCTGCACACC AGCAGCAGCA ACAGCAGTTG CAGCAGCAGC AACAACTGCT TCAGTTGTCC
    ATCCAACAGG CGGCTCACCA TCATCAGCAG GAGCAGCATC GTCAACAGCA GCAACAGCAA
    CACCAGCAGC AACAACAGCA ACAGCATCAC CAGCAGCAAC AGCAGGGTCA TCCACAGGCC
    CCGCCACCGC AGCAGCAACA GCAGCCGCCG CCCATCGCCT TGATCAGTGA CCCAAGTGCT
    CTGGCACGTG CCGCCATCCA GCTGCAGCAT CTGCCAGCGA ACGTGGAACA GCACCCGGTT
    GTTTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245993.1
    CDS1309..4197
    Translation

    Target ORF information:

    RefSeq Version NM_001259064.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crooked legs (crol), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259064.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGTCAAAC GCCGCACCAG TGCGATGTCT GCGGCAAGAA ATATACGCGC
    AAGGAGCACC TAGCCAATCA TATGCGATCC CATACCAACG AGACGCCGTT CCGTTGCGAG
    ATCTGCGGCA AGAGCTTTAG CCGCAAGGAG CACTTCACCA ATCACATACT TTGGCATACA
    GGCGAGACGC CGCACCGGTG CGACTTTTGC TCCAAGACGT TTACGCGAAA GGAGCACTTG
    CTTAACCACG TGCGCCAGCA TACGGGAGAG TCGCCACACC GCTGCTCCTA CTGCATGAAG
    ACGTTCACGC GCAAGGAGCA CCTGGTCAAC CACATACGCC AGCACACGGG TGAGACACCG
    TTCAAGTGCA CGTACTGCAC GAAAGCGTTC ACGCGCAAAG ATCACATGGT TAATCATGTA
    CGGCAACATA CAGGCGAGTC GCCGCACAAG TGCACGTACT GCACCAAGAC GTTCACGCGC
    AAGGAGCACC TGACGAACCA TGTGCGCCAG CACACGGGCG ACTCCCCGCA CCGCTGCTCC
    TACTGCAAGA AGACCTTCAC GCGCAAGGAG CACCTGACGA ACCATGTGCG CCTGCACACG
    GGCGACTCGC CGCACAAGTG CGAGTACTGC CAGAAGACGT TTACGCGGAA GGAGCACCTC
    AACAATCATA TGCGCCAGCA TTCGAGCGAC AATCCGCATT GCTGCAACGT TTGCAACAAG
    CCGTTTACGC GCAAGGAACA CCTGATCAAC CATATGTCGC GGTGCCACAC CGGTGACCGG
    CCCTTCACCT GCGAGACGTG CGGCAAATCG TTCCCGCTCA AGGGCAATCT GCTCTTCCAT
    CAGCGCAGCC ATACCAAGGG CCAGGAGATG GAGCGGCCAT TCGCCTGCGA GAAGTGCCCC
    AAGAACTTCA TCTGCAAAGG TCACTTGGTC TCGCACATGC GCTCCCATTC GGGTGAGAAA
    CCACACGCGT GCACACTGTG CAGCAAGGCG TTCGTCGAGC GCGGCAATTT GAAGCGCCAC
    ATGAAGATGA ATCACCCGGA TGCTATGATG CCGCCACCAC CCGTGCATCC GCATCCGCAA
    ATACCGGCTG GTGTGCTGAC GCAAGTCAAG CAGGAAGTGA AACCGATCAT AATTCCCCAT
    CACTCGGCGA CCACCACAAT GCACACCATT CAGCAGATCA CGGCGGGTGC GGCGGGCGGA
    GCCGGTGCGG TCCAGTTAAC ACCGGGTCTG GTGCCCCTGG TTACCTCTAC GCTCATCTCA
    CATAATGCGG CTGCTCAACA GCAGTCGCAG AAGCAGCAAG CAGCCGCCGC AGCAGCTGCA
    CAGCAACAGG CCGCAGCCGC CGCTGCTGCC CAACAGCAAG CAGCTCAGCA ACAGGCGGCT
    GCTGCACATC AGCAGCATCA ACAACAAGTG GCCGCGCAAC ATCAACAGCA GGCTGCAGTG
    GCTGCACACC AGCAGCAGCA ACAGCAGTTG CAGCAGCAGC AACAACTGCT TCAGTTGTCC
    ATCCAACAGG CGGCTCACCA TCATCAGCAG GAGCAGCATC GTCAACAGCA GCAACAGCAA
    CACCAGCAGC AACAACAGCA ACAGCATCAC CAGCAGCAAC AGCAGGGTCA TCCACAGGCC
    CCGCCACCGC AGCAGCAACA GCAGCCGCCG CCCATCGCCT TGATCAGTGA CCCAAGTGCT
    CTGGCACGTG CCGCCATCCA GCTGCAGCAT CTGCCAGCGA ACGTGGAACA GCACCCGGTT
    GTTTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14470
    Clone ID Related Accession (Same CDS sequence) NM_001259064.2 , NM_001273464.1 , NM_057895.4
    Accession Version NM_001273464.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crooked legs, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGTCAAAC GCCGCACCAG TGCGATGTCT GCGGCAAGAA ATATACGCGC
    AAGGAGCACC TAGCCAATCA TATGCGATCC CATACCAACG AGACGCCGTT CCGTTGCGAG
    ATCTGCGGCA AGAGCTTTAG CCGCAAGGAG CACTTCACCA ATCACATACT TTGGCATACA
    GGCGAGACGC CGCACCGGTG CGACTTTTGC TCCAAGACGT TTACGCGAAA GGAGCACTTG
    CTTAACCACG TGCGCCAGCA TACGGGAGAG TCGCCACACC GCTGCTCCTA CTGCATGAAG
    ACGTTCACGC GCAAGGAGCA CCTGGTCAAC CACATACGCC AGCACACGGG TGAGACACCG
    TTCAAGTGCA CGTACTGCAC GAAAGCGTTC ACGCGCAAAG ATCACATGGT TAATCATGTA
    CGGCAACATA CAGGCGAGTC GCCGCACAAG TGCACGTACT GCACCAAGAC GTTCACGCGC
    AAGGAGCACC TGACGAACCA TGTGCGCCAG CACACGGGCG ACTCCCCGCA CCGCTGCTCC
    TACTGCAAGA AGACCTTCAC GCGCAAGGAG CACCTGACGA ACCATGTGCG CCTGCACACG
    GGCGACTCGC CGCACAAGTG CGAGTACTGC CAGAAGACGT TTACGCGGAA GGAGCACCTC
    AACAATCATA TGCGCCAGCA TTCGAGCGAC AATCCGCATT GCTGCAACGT TTGCAACAAG
    CCGTTTACGC GCAAGGAACA CCTGATCAAC CATATGTCGC GGTGCCACAC CGGTGACCGG
    CCCTTCACCT GCGAGACGTG CGGCAAATCG TTCCCGCTCA AGGGCAATCT GCTCTTCCAT
    CAGCGCAGCC ATACCAAGGG CCAGGAGATG GAGCGGCCAT TCGCCTGCGA GAAGTGCCCC
    AAGAACTTCA TCTGCAAAGG TCACTTGGTC TCGCACATGC GCTCCCATTC GGGTGAGAAA
    CCACACGCGT GCACACTGTG CAGCAAGGCG TTCGTCGAGC GCGGCAATTT GAAGCGCCAC
    ATGAAGATGA ATCACCCGGA TGCTATGATG CCGCCACCAC CCGTGCATCC GCATCCGCAA
    ATACCGGCTG GTGTGCTGAC GCAAGTCAAG CAGGAAGTGA AACCGATCAT AATTCCCCAT
    CACTCGGCGA CCACCACAAT GCACACCATT CAGCAGATCA CGGCGGGTGC GGCGGGCGGA
    GCCGGTGCGG TCCAGTTAAC ACCGGGTCTG GTGCCCCTGG TTACCTCTAC GCTCATCTCA
    CATAATGCGG CTGCTCAACA GCAGTCGCAG AAGCAGCAAG CAGCCGCCGC AGCAGCTGCA
    CAGCAACAGG CCGCAGCCGC CGCTGCTGCC CAACAGCAAG CAGCTCAGCA ACAGGCGGCT
    GCTGCACATC AGCAGCATCA ACAACAAGTG GCCGCGCAAC ATCAACAGCA GGCTGCAGTG
    GCTGCACACC AGCAGCAGCA ACAGCAGTTG CAGCAGCAGC AACAACTGCT TCAGTTGTCC
    ATCCAACAGG CGGCTCACCA TCATCAGCAG GAGCAGCATC GTCAACAGCA GCAACAGCAA
    CACCAGCAGC AACAACAGCA ACAGCATCAC CAGCAGCAAC AGCAGGGTCA TCCACAGGCC
    CCGCCACCGC AGCAGCAACA GCAGCCGCCG CCCATCGCCT TGATCAGTGA CCCAAGTGCT
    CTGGCACGTG CCGCCATCCA GCTGCAGCAT CTGCCAGCGA ACGTGGAACA GCACCCGGTT
    GTTTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260393.1
    CDS773..3661
    Translation

    Target ORF information:

    RefSeq Version NM_001273464.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crooked legs (crol), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273464.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGTCAAAC GCCGCACCAG TGCGATGTCT GCGGCAAGAA ATATACGCGC
    AAGGAGCACC TAGCCAATCA TATGCGATCC CATACCAACG AGACGCCGTT CCGTTGCGAG
    ATCTGCGGCA AGAGCTTTAG CCGCAAGGAG CACTTCACCA ATCACATACT TTGGCATACA
    GGCGAGACGC CGCACCGGTG CGACTTTTGC TCCAAGACGT TTACGCGAAA GGAGCACTTG
    CTTAACCACG TGCGCCAGCA TACGGGAGAG TCGCCACACC GCTGCTCCTA CTGCATGAAG
    ACGTTCACGC GCAAGGAGCA CCTGGTCAAC CACATACGCC AGCACACGGG TGAGACACCG
    TTCAAGTGCA CGTACTGCAC GAAAGCGTTC ACGCGCAAAG ATCACATGGT TAATCATGTA
    CGGCAACATA CAGGCGAGTC GCCGCACAAG TGCACGTACT GCACCAAGAC GTTCACGCGC
    AAGGAGCACC TGACGAACCA TGTGCGCCAG CACACGGGCG ACTCCCCGCA CCGCTGCTCC
    TACTGCAAGA AGACCTTCAC GCGCAAGGAG CACCTGACGA ACCATGTGCG CCTGCACACG
    GGCGACTCGC CGCACAAGTG CGAGTACTGC CAGAAGACGT TTACGCGGAA GGAGCACCTC
    AACAATCATA TGCGCCAGCA TTCGAGCGAC AATCCGCATT GCTGCAACGT TTGCAACAAG
    CCGTTTACGC GCAAGGAACA CCTGATCAAC CATATGTCGC GGTGCCACAC CGGTGACCGG
    CCCTTCACCT GCGAGACGTG CGGCAAATCG TTCCCGCTCA AGGGCAATCT GCTCTTCCAT
    CAGCGCAGCC ATACCAAGGG CCAGGAGATG GAGCGGCCAT TCGCCTGCGA GAAGTGCCCC
    AAGAACTTCA TCTGCAAAGG TCACTTGGTC TCGCACATGC GCTCCCATTC GGGTGAGAAA
    CCACACGCGT GCACACTGTG CAGCAAGGCG TTCGTCGAGC GCGGCAATTT GAAGCGCCAC
    ATGAAGATGA ATCACCCGGA TGCTATGATG CCGCCACCAC CCGTGCATCC GCATCCGCAA
    ATACCGGCTG GTGTGCTGAC GCAAGTCAAG CAGGAAGTGA AACCGATCAT AATTCCCCAT
    CACTCGGCGA CCACCACAAT GCACACCATT CAGCAGATCA CGGCGGGTGC GGCGGGCGGA
    GCCGGTGCGG TCCAGTTAAC ACCGGGTCTG GTGCCCCTGG TTACCTCTAC GCTCATCTCA
    CATAATGCGG CTGCTCAACA GCAGTCGCAG AAGCAGCAAG CAGCCGCCGC AGCAGCTGCA
    CAGCAACAGG CCGCAGCCGC CGCTGCTGCC CAACAGCAAG CAGCTCAGCA ACAGGCGGCT
    GCTGCACATC AGCAGCATCA ACAACAAGTG GCCGCGCAAC ATCAACAGCA GGCTGCAGTG
    GCTGCACACC AGCAGCAGCA ACAGCAGTTG CAGCAGCAGC AACAACTGCT TCAGTTGTCC
    ATCCAACAGG CGGCTCACCA TCATCAGCAG GAGCAGCATC GTCAACAGCA GCAACAGCAA
    CACCAGCAGC AACAACAGCA ACAGCATCAC CAGCAGCAAC AGCAGGGTCA TCCACAGGCC
    CCGCCACCGC AGCAGCAACA GCAGCCGCCG CCCATCGCCT TGATCAGTGA CCCAAGTGCT
    CTGGCACGTG CCGCCATCCA GCTGCAGCAT CTGCCAGCGA ACGTGGAACA GCACCCGGTT
    GTTTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14470
    Clone ID Related Accession (Same CDS sequence) NM_001259064.2 , NM_001273464.1 , NM_057895.4
    Accession Version NM_057895.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crooked legs, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057895.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGTCAAAC GCCGCACCAG TGCGATGTCT GCGGCAAGAA ATATACGCGC
    AAGGAGCACC TAGCCAATCA TATGCGATCC CATACCAACG AGACGCCGTT CCGTTGCGAG
    ATCTGCGGCA AGAGCTTTAG CCGCAAGGAG CACTTCACCA ATCACATACT TTGGCATACA
    GGCGAGACGC CGCACCGGTG CGACTTTTGC TCCAAGACGT TTACGCGAAA GGAGCACTTG
    CTTAACCACG TGCGCCAGCA TACGGGAGAG TCGCCACACC GCTGCTCCTA CTGCATGAAG
    ACGTTCACGC GCAAGGAGCA CCTGGTCAAC CACATACGCC AGCACACGGG TGAGACACCG
    TTCAAGTGCA CGTACTGCAC GAAAGCGTTC ACGCGCAAAG ATCACATGGT TAATCATGTA
    CGGCAACATA CAGGCGAGTC GCCGCACAAG TGCACGTACT GCACCAAGAC GTTCACGCGC
    AAGGAGCACC TGACGAACCA TGTGCGCCAG CACACGGGCG ACTCCCCGCA CCGCTGCTCC
    TACTGCAAGA AGACCTTCAC GCGCAAGGAG CACCTGACGA ACCATGTGCG CCTGCACACG
    GGCGACTCGC CGCACAAGTG CGAGTACTGC CAGAAGACGT TTACGCGGAA GGAGCACCTC
    AACAATCATA TGCGCCAGCA TTCGAGCGAC AATCCGCATT GCTGCAACGT TTGCAACAAG
    CCGTTTACGC GCAAGGAACA CCTGATCAAC CATATGTCGC GGTGCCACAC CGGTGACCGG
    CCCTTCACCT GCGAGACGTG CGGCAAATCG TTCCCGCTCA AGGGCAATCT GCTCTTCCAT
    CAGCGCAGCC ATACCAAGGG CCAGGAGATG GAGCGGCCAT TCGCCTGCGA GAAGTGCCCC
    AAGAACTTCA TCTGCAAAGG TCACTTGGTC TCGCACATGC GCTCCCATTC GGGTGAGAAA
    CCACACGCGT GCACACTGTG CAGCAAGGCG TTCGTCGAGC GCGGCAATTT GAAGCGCCAC
    ATGAAGATGA ATCACCCGGA TGCTATGATG CCGCCACCAC CCGTGCATCC GCATCCGCAA
    ATACCGGCTG GTGTGCTGAC GCAAGTCAAG CAGGAAGTGA AACCGATCAT AATTCCCCAT
    CACTCGGCGA CCACCACAAT GCACACCATT CAGCAGATCA CGGCGGGTGC GGCGGGCGGA
    GCCGGTGCGG TCCAGTTAAC ACCGGGTCTG GTGCCCCTGG TTACCTCTAC GCTCATCTCA
    CATAATGCGG CTGCTCAACA GCAGTCGCAG AAGCAGCAAG CAGCCGCCGC AGCAGCTGCA
    CAGCAACAGG CCGCAGCCGC CGCTGCTGCC CAACAGCAAG CAGCTCAGCA ACAGGCGGCT
    GCTGCACATC AGCAGCATCA ACAACAAGTG GCCGCGCAAC ATCAACAGCA GGCTGCAGTG
    GCTGCACACC AGCAGCAGCA ACAGCAGTTG CAGCAGCAGC AACAACTGCT TCAGTTGTCC
    ATCCAACAGG CGGCTCACCA TCATCAGCAG GAGCAGCATC GTCAACAGCA GCAACAGCAA
    CACCAGCAGC AACAACAGCA ACAGCATCAC CAGCAGCAAC AGCAGGGTCA TCCACAGGCC
    CCGCCACCGC AGCAGCAACA GCAGCCGCCG CCCATCGCCT TGATCAGTGA CCCAAGTGCT
    CTGGCACGTG CCGCCATCCA GCTGCAGCAT CTGCCAGCGA ACGTGGAACA GCACCCGGTT
    GTTTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477243.1
    CDS1419..4307
    Translation

    Target ORF information:

    RefSeq Version NM_057895.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crooked legs (crol), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057895.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TGAGACGCCA
    CATCGTTGCG ATATTTGTAA GAAGTCCTTT ACGCGCAAGG AACACTATGT TAACCACTAC
    ATGTGGCACA CTGGTCAAAC GCCGCACCAG TGCGATGTCT GCGGCAAGAA ATATACGCGC
    AAGGAGCACC TAGCCAATCA TATGCGATCC CATACCAACG AGACGCCGTT CCGTTGCGAG
    ATCTGCGGCA AGAGCTTTAG CCGCAAGGAG CACTTCACCA ATCACATACT TTGGCATACA
    GGCGAGACGC CGCACCGGTG CGACTTTTGC TCCAAGACGT TTACGCGAAA GGAGCACTTG
    CTTAACCACG TGCGCCAGCA TACGGGAGAG TCGCCACACC GCTGCTCCTA CTGCATGAAG
    ACGTTCACGC GCAAGGAGCA CCTGGTCAAC CACATACGCC AGCACACGGG TGAGACACCG
    TTCAAGTGCA CGTACTGCAC GAAAGCGTTC ACGCGCAAAG ATCACATGGT TAATCATGTA
    CGGCAACATA CAGGCGAGTC GCCGCACAAG TGCACGTACT GCACCAAGAC GTTCACGCGC
    AAGGAGCACC TGACGAACCA TGTGCGCCAG CACACGGGCG ACTCCCCGCA CCGCTGCTCC
    TACTGCAAGA AGACCTTCAC GCGCAAGGAG CACCTGACGA ACCATGTGCG CCTGCACACG
    GGCGACTCGC CGCACAAGTG CGAGTACTGC CAGAAGACGT TTACGCGGAA GGAGCACCTC
    AACAATCATA TGCGCCAGCA TTCGAGCGAC AATCCGCATT GCTGCAACGT TTGCAACAAG
    CCGTTTACGC GCAAGGAACA CCTGATCAAC CATATGTCGC GGTGCCACAC CGGTGACCGG
    CCCTTCACCT GCGAGACGTG CGGCAAATCG TTCCCGCTCA AGGGCAATCT GCTCTTCCAT
    CAGCGCAGCC ATACCAAGGG CCAGGAGATG GAGCGGCCAT TCGCCTGCGA GAAGTGCCCC
    AAGAACTTCA TCTGCAAAGG TCACTTGGTC TCGCACATGC GCTCCCATTC GGGTGAGAAA
    CCACACGCGT GCACACTGTG CAGCAAGGCG TTCGTCGAGC GCGGCAATTT GAAGCGCCAC
    ATGAAGATGA ATCACCCGGA TGCTATGATG CCGCCACCAC CCGTGCATCC GCATCCGCAA
    ATACCGGCTG GTGTGCTGAC GCAAGTCAAG CAGGAAGTGA AACCGATCAT AATTCCCCAT
    CACTCGGCGA CCACCACAAT GCACACCATT CAGCAGATCA CGGCGGGTGC GGCGGGCGGA
    GCCGGTGCGG TCCAGTTAAC ACCGGGTCTG GTGCCCCTGG TTACCTCTAC GCTCATCTCA
    CATAATGCGG CTGCTCAACA GCAGTCGCAG AAGCAGCAAG CAGCCGCCGC AGCAGCTGCA
    CAGCAACAGG CCGCAGCCGC CGCTGCTGCC CAACAGCAAG CAGCTCAGCA ACAGGCGGCT
    GCTGCACATC AGCAGCATCA ACAACAAGTG GCCGCGCAAC ATCAACAGCA GGCTGCAGTG
    GCTGCACACC AGCAGCAGCA ACAGCAGTTG CAGCAGCAGC AACAACTGCT TCAGTTGTCC
    ATCCAACAGG CGGCTCACCA TCATCAGCAG GAGCAGCATC GTCAACAGCA GCAACAGCAA
    CACCAGCAGC AACAACAGCA ACAGCATCAC CAGCAGCAAC AGCAGGGTCA TCCACAGGCC
    CCGCCACCGC AGCAGCAACA GCAGCCGCCG CCCATCGCCT TGATCAGTGA CCCAAGTGCT
    CTGGCACGTG CCGCCATCCA GCTGCAGCAT CTGCCAGCGA ACGTGGAACA GCACCCGGTT
    GTTTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16702
    Clone ID Related Accession (Same CDS sequence) NM_001273462.1
    Accession Version NM_001273462.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crooked legs, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG CGAGACGCCG
    CACCGGTGCG ACTTTTGCTC CAAGACGTTT ACGCGAAAGG AGCACTTGCT TAACCACGTG
    CGCCAGCATA CGGGAGAGTC GCCACACCGC TGCTCCTACT GCATGAAGAC GTTCACGCGC
    AAGGAGCACC TGGTCAACCA CATACGCCAG CACACGGGTG AGACACCGTT CAAGTGCACG
    TACTGCACGA AAGCGTTCAC GCGCAAAGAT CACATGGTTA ATCATGTACG GCAACATACA
    GGCGAGTCGC CGCACAAGTG CACGTACTGC ACCAAGACGT TCACGCGCAA GGAGCACCTG
    ACGAACCATG TGCGCCAGCA CACGGGCGAC TCCCCGCACC GCTGCTCCTA CTGCAAGAAG
    ACCTTCACGC GCAAGGAGCA CCTGACGAAC CATGTGCGCC TGCACACGGG CGACTCGCCG
    CACAAGTGCG AGTACTGCCA GAAGACGTTT ACGCGGAAGG AGCACCTCAA CAATCATATG
    CGCCAGCATT CGAGCGACAA TCCGCATTGC TGCAACGTTT GCAACAAGCC GTTTACGCGC
    AAGGAACACC TGATCAACCA TATGTCGCGG TGCCACACCG GTGACCGGCC CTTCACCTGC
    GAGACGTGCG GCAAATCGTT CCCGCTCAAG GGCAATCTGC TCTTCCATCA GCGCAGCCAT
    ACCAAGGGCC AGGAGATGGA GCGGCCATTC GCCTGCGAGA AGTGCCCCAA GAACTTCATC
    TGCAAAGGTC ACTTGGTCTC GCACATGCGC TCCCATTCGG GTGAGAAACC ACACGCGTGC
    ACACTGTGCA GCAAGGCGTT CGTCGAGCGC GGCAATTTGA AGCGCCACAT GAAGATGAAT
    CACCCGGATG CTATGATGCC GCCACCACCC GTGCATCCGC ATCCGCAAAT ACCGGCTGGT
    GTGCTGACGC AAGTCAAGCA GGAAGTGAAA CCGATCATAA TTCCCCATCA CTCGGCGACC
    ACCACAATGC ACACCATTCA GCAGATCACG GCGGGTGCGG CGGGCGGAGC CGGTGCGGTC
    CAGTTAACAC CGGGTCTGGT GCCCCTGGTT ACCTCTACGC TCATCTCACA TAATGCGGCT
    GCTCAACAGC AGTCGCAGAA GCAGCAAGCA GCCGCCGCAG CAGCTGCACA GCAACAGGCC
    GCAGCCGCCG CTGCTGCCCA ACAGCAAGCA GCTCAGCAAC AGGCGGCTGC TGCACATCAG
    CAGCATCAAC AACAAGTGGC CGCGCAACAT CAACAGCAGG CTGCAGTGGC TGCACACCAG
    CAGCAGCAAC AGCAGTTGCA GCAGCAGCAA CAACTGCTTC AGTTGTCCAT CCAACAGGCG
    GCTCACCATC ATCAGCAGGA GCAGCATCGT CAACAGCAGC AACAGCAACA CCAGCAGCAA
    CAACAGCAAC AGCATCACCA GCAGCAACAG CAGGGTCATC CACAGGCCCC GCCACCGCAG
    CAGCAACAGC AGCCGCCGCC CATCGCCTTG ATCAGTGACC CAAGTGCTCT GGCACGTGCC
    GCCATCCAGC TGCAGCATCT GCCAGCGAAC GTGGAACAGC ACCCGGTTGT TTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260391.1
    CDS663..3299
    Translation

    Target ORF information:

    RefSeq Version NM_001273462.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crooked legs (crol), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273462.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG CGAGACGCCG
    CACCGGTGCG ACTTTTGCTC CAAGACGTTT ACGCGAAAGG AGCACTTGCT TAACCACGTG
    CGCCAGCATA CGGGAGAGTC GCCACACCGC TGCTCCTACT GCATGAAGAC GTTCACGCGC
    AAGGAGCACC TGGTCAACCA CATACGCCAG CACACGGGTG AGACACCGTT CAAGTGCACG
    TACTGCACGA AAGCGTTCAC GCGCAAAGAT CACATGGTTA ATCATGTACG GCAACATACA
    GGCGAGTCGC CGCACAAGTG CACGTACTGC ACCAAGACGT TCACGCGCAA GGAGCACCTG
    ACGAACCATG TGCGCCAGCA CACGGGCGAC TCCCCGCACC GCTGCTCCTA CTGCAAGAAG
    ACCTTCACGC GCAAGGAGCA CCTGACGAAC CATGTGCGCC TGCACACGGG CGACTCGCCG
    CACAAGTGCG AGTACTGCCA GAAGACGTTT ACGCGGAAGG AGCACCTCAA CAATCATATG
    CGCCAGCATT CGAGCGACAA TCCGCATTGC TGCAACGTTT GCAACAAGCC GTTTACGCGC
    AAGGAACACC TGATCAACCA TATGTCGCGG TGCCACACCG GTGACCGGCC CTTCACCTGC
    GAGACGTGCG GCAAATCGTT CCCGCTCAAG GGCAATCTGC TCTTCCATCA GCGCAGCCAT
    ACCAAGGGCC AGGAGATGGA GCGGCCATTC GCCTGCGAGA AGTGCCCCAA GAACTTCATC
    TGCAAAGGTC ACTTGGTCTC GCACATGCGC TCCCATTCGG GTGAGAAACC ACACGCGTGC
    ACACTGTGCA GCAAGGCGTT CGTCGAGCGC GGCAATTTGA AGCGCCACAT GAAGATGAAT
    CACCCGGATG CTATGATGCC GCCACCACCC GTGCATCCGC ATCCGCAAAT ACCGGCTGGT
    GTGCTGACGC AAGTCAAGCA GGAAGTGAAA CCGATCATAA TTCCCCATCA CTCGGCGACC
    ACCACAATGC ACACCATTCA GCAGATCACG GCGGGTGCGG CGGGCGGAGC CGGTGCGGTC
    CAGTTAACAC CGGGTCTGGT GCCCCTGGTT ACCTCTACGC TCATCTCACA TAATGCGGCT
    GCTCAACAGC AGTCGCAGAA GCAGCAAGCA GCCGCCGCAG CAGCTGCACA GCAACAGGCC
    GCAGCCGCCG CTGCTGCCCA ACAGCAAGCA GCTCAGCAAC AGGCGGCTGC TGCACATCAG
    CAGCATCAAC AACAAGTGGC CGCGCAACAT CAACAGCAGG CTGCAGTGGC TGCACACCAG
    CAGCAGCAAC AGCAGTTGCA GCAGCAGCAA CAACTGCTTC AGTTGTCCAT CCAACAGGCG
    GCTCACCATC ATCAGCAGGA GCAGCATCGT CAACAGCAGC AACAGCAACA CCAGCAGCAA
    CAACAGCAAC AGCATCACCA GCAGCAACAG CAGGGTCATC CACAGGCCCC GCCACCGCAG
    CAGCAACAGC AGCCGCCGCC CATCGCCTTG ATCAGTGACC CAAGTGCTCT GGCACGTGCC
    GCCATCCAGC TGCAGCATCT GCCAGCGAAC GTGGAACAGC ACCCGGTTGT TTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16704
    Clone ID Related Accession (Same CDS sequence) NM_001273461.1
    Accession Version NM_001273461.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2724bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crooked legs, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TCAAACGCCG
    CACCAGTGCG ATGTCTGCGG CAAGAAATAT ACGCGCAAGG AGCACCTAGC CAATCATATG
    CGATCCCATA CCAACGAGAC GCCGTTCCGT TGCGAGATCT GCGGCAAGAG CTTTAGCCGC
    AAGGAGCACT TCACCAATCA CATACTTTGG CATACAGCAG GCGAGACGCC GCACCGGTGC
    GACTTTTGCT CCAAGACGTT TACGCGAAAG GAGCACTTGC TTAACCACGT GCGCCAGCAT
    ACGGGAGAGT CGCCACACCG CTGCTCCTAC TGCATGAAGA CGTTCACGCG CAAGGAGCAC
    CTGGTCAACC ACATACGCCA GCACACGGGC GAGTCGCCGC ACAAGTGCAC GTACTGCACC
    AAGACGTTCA CGCGCAAGGA GCACCTGACG AACCATGTGC GCCAGCACAC GGGCGACTCC
    CCGCACCGCT GCTCCTACTG CAAGAAGACC TTCACGCGCA AGGAGCACCT GACGAACCAT
    GTGCGCCTGC ACACGGGCGA CTCGCCGCAC AAGTGCGAGT ACTGCCAGAA GACGTTTACG
    CGGAAGGAGC ACCTCAACAA TCATATGCGC CAGCATTCGA GCGACAATCC GCATTGCTGC
    AACGTTTGCA ACAAGCCGTT TACGCGCAAG GAACACCTGA TCAACCATAT GTCGCGGTGC
    CACACCGGTG ACCGGCCCTT CACCTGCGAG ACGTGCGGCA AATCGTTCCC GCTCAAGGGC
    AATCTGCTCT TCCATCAGCG CAGCCATACC AAGGGCCAGG AGATGGAGCG GCCATTCGCC
    TGCGAGAAGT GCCCCAAGAA CTTCATCTGC AAAGGTCACT TGGTCTCGCA CATGCGCTCC
    CATTCGGGTG AGAAACCACA CGCGTGCACA CTGTGCAGCA AGGCGTTCGT CGAGCGCGGC
    AATTTGAAGC GCCACATGAA GATGAATCAC CCGGATGCTA TGATGCCGCC ACCACCCGTG
    CATCCGCATC CGCAAATACC GGCTGGTGTG CTGACGCAAG TCAAGCAGGA AGTGAAACCG
    ATCATAATTC CCCATCACTC GGCGACCACC ACAATGCACA CCATTCAGCA GATCACGGCG
    GGTGCGGCGG GCGGAGCCGG TGCGGTCCAG TTAACACCGG GTCTGGTGCC CCTGGTTACC
    TCTACGCTCA TCTCACATAA TGCGGCTGCT CAACAGCAGT CGCAGAAGCA GCAAGCAGCC
    GCCGCAGCAG CTGCACAGCA ACAGGCCGCA GCCGCCGCTG CTGCCCAACA GCAAGCAGCT
    CAGCAACAGG CGGCTGCTGC ACATCAGCAG CATCAACAAC AAGTGGCCGC GCAACATCAA
    CAGCAGGCTG CAGTGGCTGC ACACCAGCAG CAGCAACAGC AGTTGCAGCA GCAGCAACAA
    CTGCTTCAGT TGTCCATCCA ACAGGCGGCT CACCATCATC AGCAGGAGCA GCATCGTCAA
    CAGCAGCAAC AGCAACACCA GCAGCAACAA CAGCAACAGC ATCACCAGCA GCAACAGCAG
    GGTCATCCAC AGGCCCCGCC ACCGCAGCAG CAACAGCAGC CGCCGCCCAT CGCCTTGATC
    AGTGACCCAA GTGCTCTGGC ACGTGCCGCC ATCCAGCTGC AGCATCTGCC AGCGAACGTG
    GAACAGCACC CGGTTGTTTA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260390.1
    CDS663..3386
    Translation

    Target ORF information:

    RefSeq Version NM_001273461.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crooked legs (crol), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273461.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTGAG
    TTGCTTATGC CTAAAATGGA GGGCGGAATT CATGGCGTGG ACGGCAGCGG CAATGGCGGC
    AATGGTGGCG GACAAAACGT GGCGCTGGCG CCAGACGGTA CGCCCATTGC GACGGGGACG
    CACGTCTGCG ACATCTGCGG CAAGATGTTC CAGTTCCGGT ACCAGCTGAT CGTGCACCGG
    CGCTACCACA GCGAACGGAA GCCGTTCATG TGCCAGGTGT GCGGCCAGGG GTTCACCACG
    TCGCAGGATT TGACGCGCCA CGGCAAGATC CATATTGGCG GGCCCATGTT CACCTGCATC
    GTGTGCTTCA ATGTGTTCGC GAACAATACG AGCCTGGAGC GGCACATGAA ACGGCACTCG
    ACGGACAAAC CGTTCGCCTG CACCATTTGC CAAAAGACCT TTGCCCGCAA AGAGCATCTG
    GACAATCACT TCCGCTCGCA CACGGGCGAA ACGCCCTTCC GTTGCCAGTA CTGCGCCAAG
    ACGTTCACGC GCAAGGAGCA CATGGTCAAC CATGTGCGCA AACACACGGG TCAAACGCCG
    CACCAGTGCG ATGTCTGCGG CAAGAAATAT ACGCGCAAGG AGCACCTAGC CAATCATATG
    CGATCCCATA CCAACGAGAC GCCGTTCCGT TGCGAGATCT GCGGCAAGAG CTTTAGCCGC
    AAGGAGCACT TCACCAATCA CATACTTTGG CATACAGCAG GCGAGACGCC GCACCGGTGC
    GACTTTTGCT CCAAGACGTT TACGCGAAAG GAGCACTTGC TTAACCACGT GCGCCAGCAT
    ACGGGAGAGT CGCCACACCG CTGCTCCTAC TGCATGAAGA CGTTCACGCG CAAGGAGCAC
    CTGGTCAACC ACATACGCCA GCACACGGGC GAGTCGCCGC ACAAGTGCAC GTACTGCACC
    AAGACGTTCA CGCGCAAGGA GCACCTGACG AACCATGTGC GCCAGCACAC GGGCGACTCC
    CCGCACCGCT GCTCCTACTG CAAGAAGACC TTCACGCGCA AGGAGCACCT GACGAACCAT
    GTGCGCCTGC ACACGGGCGA CTCGCCGCAC AAGTGCGAGT ACTGCCAGAA GACGTTTACG
    CGGAAGGAGC ACCTCAACAA TCATATGCGC CAGCATTCGA GCGACAATCC GCATTGCTGC
    AACGTTTGCA ACAAGCCGTT TACGCGCAAG GAACACCTGA TCAACCATAT GTCGCGGTGC
    CACACCGGTG ACCGGCCCTT CACCTGCGAG ACGTGCGGCA AATCGTTCCC GCTCAAGGGC
    AATCTGCTCT TCCATCAGCG CAGCCATACC AAGGGCCAGG AGATGGAGCG GCCATTCGCC
    TGCGAGAAGT GCCCCAAGAA CTTCATCTGC AAAGGTCACT TGGTCTCGCA CATGCGCTCC
    CATTCGGGTG AGAAACCACA CGCGTGCACA CTGTGCAGCA AGGCGTTCGT CGAGCGCGGC
    AATTTGAAGC GCCACATGAA GATGAATCAC CCGGATGCTA TGATGCCGCC ACCACCCGTG
    CATCCGCATC CGCAAATACC GGCTGGTGTG CTGACGCAAG TCAAGCAGGA AGTGAAACCG
    ATCATAATTC CCCATCACTC GGCGACCACC ACAATGCACA CCATTCAGCA GATCACGGCG
    GGTGCGGCGG GCGGAGCCGG TGCGGTCCAG TTAACACCGG GTCTGGTGCC CCTGGTTACC
    TCTACGCTCA TCTCACATAA TGCGGCTGCT CAACAGCAGT CGCAGAAGCA GCAAGCAGCC
    GCCGCAGCAG CTGCACAGCA ACAGGCCGCA GCCGCCGCTG CTGCCCAACA GCAAGCAGCT
    CAGCAACAGG CGGCTGCTGC ACATCAGCAG CATCAACAAC AAGTGGCCGC GCAACATCAA
    CAGCAGGCTG CAGTGGCTGC ACACCAGCAG CAGCAACAGC AGTTGCAGCA GCAGCAACAA
    CTGCTTCAGT TGTCCATCCA ACAGGCGGCT CACCATCATC AGCAGGAGCA GCATCGTCAA
    CAGCAGCAAC AGCAACACCA GCAGCAACAA CAGCAACAGC ATCACCAGCA GCAACAGCAG
    GGTCATCCAC AGGCCCCGCC ACCGCAGCAG CAACAGCAGC CGCCGCCCAT CGCCTTGATC
    AGTGACCCAA GTGCTCTGGC ACGTGCCGCC ATCCAGCTGC AGCATCTGCC AGCGAACGTG
    GAACAGCACC CGGTTGTTTA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16705
    Clone ID Related Accession (Same CDS sequence) NM_001273463.1
    Accession Version NM_001273463.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crooked legs, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTCAA
    ACGCCGCACC AGTGCGATGT CTGCGGCAAG AAATATACGC GCAAGGAGCA CCTAGCCAAT
    CATATGCGAT CCCATACCAA CGAGACGCCG TTCCGTTGCG AGATCTGCGG CAAGAGCTTT
    AGCCGCAAGG AGCACTTCAC CAATCACATA CTTTGGCATA CAGCAGGCGA GACGCCGCAC
    CGGTGCGACT TTTGCTCCAA GACGTTTACG CGAAAGGAGC ACTTGCTTAA CCACGTGCGC
    CAGCATACGG GAGAGTCGCC ACACCGCTGC TCCTACTGCA TGAAGACGTT CACGCGCAAG
    GAGCACCTGG TCAACCACAT ACGCCAGCAC ACGGGTGAGA CACCGTTCAA GTGCACGTAC
    TGCACGAAAG CGTTCACGCG CAAAGATCAC ATGGTTAATC ATGTACGGCA ACATACAGGC
    GAGTCGCCGC ACAAGTGCAC GTACTGCACC AAGACGTTCA CGCGCAAGGA GCACCTGACG
    AACCATGTGC GCCAGCACAC GGGCGACTCC CCGCACCGCT GCTCCTACTG CAAGAAGACC
    TTCACGCGCA AGGAGCACCT GACGAACCAT GTGCGCCTGC ACACGGGCGA CTCGCCGCAC
    AAGTGCGAGT ACTGCCAGAA GACGTTTACG CGGAAGGAGC ACCTCAACAA TCATATGCGC
    CAGCATTCGA GCGACAATCC GCATTGCTGC AACGTTTGCA ACAAGCCGTT TACGCGCAAG
    GAACACCTGA TCAACCATAT GTCGCGGTGC CACACCGGTG ACCGGCCCTT CACCTGCGAG
    ACGTGCGGCA AATCGTTCCC GCTCAAGGGC AATCTGCTCT TCCATCAGCG CAGCCATACC
    AAGGGCCAGG AGATGGAGCG GCCATTCGCC TGCGAGAAGT GCCCCAAGAA CTTCATCTGC
    AAAGTTCCCC ATCACTCGGC GACCACCACA ATGCACACCA TTCAGCAGAT CACGGCGGGT
    GCGGCGGGCG GAGCCGGTGC GGTCCAGTTA ACACCGGGTC TGGTGCCCCT GGTTACCTCT
    ACGCTCATCT CACATAATGC GGCTGCTCAA CAGCAGTCGC AGAAGCAGCA AGCAGCCGCC
    GCAGCAGCTG CACAGCAACA GGCCGCAGCC GCCGCTGCTG CCCAACAGCA AGCAGCTCAG
    CAACAGGCGG CTGCTGCACA TCAGCAGCAT CAACAACAAG TGGCCGCGCA ACATCAACAG
    CAGGCTGCAG TGGCTGCACA CCAGCAGCAG CAACAGCAGT TGCAGCAGCA GCAACAACTG
    CTTCAGTTGT CCATCCAACA GGCGGCTCAC CATCATCAGC AGGAGCAGCA TCGTCAACAG
    CAGCAACAGC AACACCAGCA GCAACAACAG CAACAGCATC ACCAGCAGCA ACAGCAGGGT
    CATCCACAGG CCCCGCCACC GCAGCAGCAA CAGCAGCCGC CGCCCATCGC CTTGATCAGT
    GACCCAAGTG CTCTGGCACG TGCCGCCATC CAGCTGCAGC ATCTGCCAGC GAACGTGGAA
    CAGCACCCGG TTGTTTACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260392.1
    CDS773..2833
    Translation

    Target ORF information:

    RefSeq Version NM_001273463.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crooked legs (crol), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273463.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCAGCACG TGAGCGCTGC CAGCTCGGTG CCATCAGTAG TAACTCCTGT TGTGACCACT 
    GGTGGGACAA CGATCACATT GGGCGGCCCA CCACCGCTCC CTAAATCCGA GCACAAAGAG
    GATGGCAAGC CGCCGCACGG CATCGAAATG TACAAGGTGA ACATTGAGGA CATTTCGCAG
    CTCTTCACCT ACCACGAGGT CTTTGGCAAA ATCCACGGCG ATGTGGTCAA TCATCAATTA
    GCAGCGGCCC ACGGCGGCCA GTTGCCACCA CCTCCTCCGC TGCCGCCGCA GGTTACCAGT
    CATGCGGCGA GTGCAGCGGC AGCCGCAGCA GCGGCGTCCA CGAATAATGC CGCCGTTGCA
    GCGGTAATGG CATCAGCGAA TGCAGCAGCG GCCGCGGCGG CAGCTGCATC GGCGGGGGGA
    GGACTACCGC CGGCCACCAG CGGCAATGGG GGCCAGCAGG TGACGGTGAC GACGACCAGC
    AGCTCGACTA GCAGCGGCGG GAGCACCACC AGTGGGGGCA CCACGACCAC GGCGGGTCAA
    ACGCCGCACC AGTGCGATGT CTGCGGCAAG AAATATACGC GCAAGGAGCA CCTAGCCAAT
    CATATGCGAT CCCATACCAA CGAGACGCCG TTCCGTTGCG AGATCTGCGG CAAGAGCTTT
    AGCCGCAAGG AGCACTTCAC CAATCACATA CTTTGGCATA CAGCAGGCGA GACGCCGCAC
    CGGTGCGACT TTTGCTCCAA GACGTTTACG CGAAAGGAGC ACTTGCTTAA CCACGTGCGC
    CAGCATACGG GAGAGTCGCC ACACCGCTGC TCCTACTGCA TGAAGACGTT CACGCGCAAG
    GAGCACCTGG TCAACCACAT ACGCCAGCAC ACGGGTGAGA CACCGTTCAA GTGCACGTAC
    TGCACGAAAG CGTTCACGCG CAAAGATCAC ATGGTTAATC ATGTACGGCA ACATACAGGC
    GAGTCGCCGC ACAAGTGCAC GTACTGCACC AAGACGTTCA CGCGCAAGGA GCACCTGACG
    AACCATGTGC GCCAGCACAC GGGCGACTCC CCGCACCGCT GCTCCTACTG CAAGAAGACC
    TTCACGCGCA AGGAGCACCT GACGAACCAT GTGCGCCTGC ACACGGGCGA CTCGCCGCAC
    AAGTGCGAGT ACTGCCAGAA GACGTTTACG CGGAAGGAGC ACCTCAACAA TCATATGCGC
    CAGCATTCGA GCGACAATCC GCATTGCTGC AACGTTTGCA ACAAGCCGTT TACGCGCAAG
    GAACACCTGA TCAACCATAT GTCGCGGTGC CACACCGGTG ACCGGCCCTT CACCTGCGAG
    ACGTGCGGCA AATCGTTCCC GCTCAAGGGC AATCTGCTCT TCCATCAGCG CAGCCATACC
    AAGGGCCAGG AGATGGAGCG GCCATTCGCC TGCGAGAAGT GCCCCAAGAA CTTCATCTGC
    AAAGTTCCCC ATCACTCGGC GACCACCACA ATGCACACCA TTCAGCAGAT CACGGCGGGT
    GCGGCGGGCG GAGCCGGTGC GGTCCAGTTA ACACCGGGTC TGGTGCCCCT GGTTACCTCT
    ACGCTCATCT CACATAATGC GGCTGCTCAA CAGCAGTCGC AGAAGCAGCA AGCAGCCGCC
    GCAGCAGCTG CACAGCAACA GGCCGCAGCC GCCGCTGCTG CCCAACAGCA AGCAGCTCAG
    CAACAGGCGG CTGCTGCACA TCAGCAGCAT CAACAACAAG TGGCCGCGCA ACATCAACAG
    CAGGCTGCAG TGGCTGCACA CCAGCAGCAG CAACAGCAGT TGCAGCAGCA GCAACAACTG
    CTTCAGTTGT CCATCCAACA GGCGGCTCAC CATCATCAGC AGGAGCAGCA TCGTCAACAG
    CAGCAACAGC AACACCAGCA GCAACAACAG CAACAGCATC ACCAGCAGCA ACAGCAGGGT
    CATCCACAGG CCCCGCCACC GCAGCAGCAA CAGCAGCCGC CGCCCATCGC CTTGATCAGT
    GACCCAAGTG CTCTGGCACG TGCCGCCATC CAGCTGCAGC ATCTGCCAGC GAACGTGGAA
    CAGCACCCGG TTGTTTACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.