ab cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ab
Entrez Gene ID	34560
Full Name	abrupt
Synonyms	Ab,B2149,CG32830,CG43860,CG4807,Dmel\CG43860,Dmel_CG32830,Dmel_CG4807,clu,l(2)k02807,ptd
General protein information	Preferred Names abrupt Names CG43860 gene product from transcript CG43860-RA CG43860-PA CG43860-PB CG43860-PD CG43860-PE CG43860-PF ab-PA ab-PB ab-PD ab-PE ab-PF clueless parted
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32E1-32E2\|2-44.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169481.2	NP_001162952.1	abrupt, isoform B
NM_001273450.1	NP_001260379.1	abrupt, isoform F
NM_057214.5	NP_476562.1	abrupt, isoform A
NM_001273449.1	NP_001260378.1	abrupt, isoform E
NM_057215.5	NP_476563.1	abrupt, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ab is essential for proper wing vein patterning both in Tribolium and Drosophila.
Title: Comparative developmental analysis of Drosophila and Tribolium reveals conserved and diverged roles of abrupt in insect wing evolution.

The transcription factors Abrupt (Ab) and Knot (Kn) act as selectors of distinct dendritic arbor morphologies in two classes of Drosophila sensory neurons, termed class I and class IV, respectively.
Title: Sensory-neuron subtype-specific transcriptional programs controlling dendrite morphogenesis: genome-wide analysis of Abrupt and Knot/Collier.

Abrupt is a repressor of ecdysone signalling and border-cell migration in ovarian follicles. Abrupt represents a node of integration for steroid hormone and JAK-STAT signals.
Title: Border-cell migration requires integration of spatial and temporal signals by the BTB protein Abrupt.

The dendritic branching can be suppressed by neuronal subtype-specific transcription factors in a cell-autonomous and dosage-dependent manner.
Title: BTB/POZ-zinc finger protein abrupt suppresses dendritic branching in a neuronal subtype-specific and dosage-dependent manner.

Ab endows characteristics of dendritic morphogenesis of the class I ofdendritic arborization (da)neurons.
Title: Development of morphological diversity of dendrites in Drosophila by the BTB-zinc finger protein abrupt.

The following ab gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ab cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14456	NM_001273450.1 Latest version!	Drosophila melanogaster abrupt (ab), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa14456	NM_057215.5 Latest version!	Drosophila melanogaster abrupt (ab), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa14456	NM_001169481.2 Latest version!	Drosophila melanogaster abrupt (ab), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa14457	NM_001273449.1 Latest version!	Drosophila melanogaster abrupt (ab), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa16675	NM_057214.5 Latest version!	Drosophila melanogaster abrupt (ab), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ab ( NM_001169481.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162952.1 Drosophila melanogaster abrupt (ab), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14456
Clone ID Related Accession (Same CDS sequence)	NM_001169481.2 , NM_001273450.1 , NM_057215.5
Accession Version	NM_001169481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	abrupt, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169481.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGCCAG CAGCAGCAAC 
CACTGGGACA TGGGCGAGAT GGAGGGCAGC CGGAAGAGCC ATCTGACGCC GCCGCCACAG 
AAACGCATCA AGAGCGCCGA CTTGTTCCGT GCCCAGCATG GCATCAGTCC CGAGCGATTG 
CTGTTGGATC GGGAGTTCCC CGTCGCCGGA CAGCATCCAC TCACAAGGAA CAGAAGCGGT 
CGGGACACAT CCAAGGATCG GGAGCGCAAC TTGGAGTTGA GGGAATCGCT ACTGGGACAG 
GCTTTGGAGA ACAGCAACGG CCAGCAGGCC AATCCGAAAC ACGAACTGGG ACAGAGCGCG 
GGTGAAGATT CGAACAGCAG TGATACCGAG CCCTCGGATC GCGGCGATGG TCAGCACGAT 
GGAACCCTCG ACGGGATTGA CAACCAGCGC TCGCACTCGT TCCCCAATGC ATTCCTCGGC 
CTCCAGGGCA TTCCAGGACT TCTGCCAGGA CCCTCTGGCA TCAACAGCGA TTTCGTTTCC 
CGACGCTCCC TGGAAATGCG AGTCCGAGCC ACAGATCCGC GTCCCTGCCC CAAGTGCGGA 
AAAATCTACC GCTCCGCCCA CACGCTACGC ACCCATCTGG AGGACAAGCA CACCGTGTGC 
CCCGGCTATC GATGTGTGCT CTGCGGCACG GTGGCCAAGT CCCGCAACTC TTTGCACTCG 
CATATGTCGC GCCAACATCG CGGCATCTCC ACCAAGGACC TGCCCGTCCT GCCCATGCCC 
AGTGCTTTCG ATCCAGAGCT CGCCTCGCGC CTTCTGGCCA AGGCGGGCGT CAAGATCTCA 
CCCGCTGAGC TGAGGGCCAG AGCCTCGCCC ACCGGCGGAA GTGGCAGCAG CGGCGGAGGC 
GGCGGAGGTG GTAGCAGCCA GGCCAAGTTG GATTTAAGCA ACGCTAGCGG TGGACCCATG 
GACGATGCCG AGGACTCGGA CGACGATCCC GAGGACCTGA CCACGGGCAA CGGATTGTAT 
GGAATGGGCG GAAGCAGCAG CGACCTGAGC CGTTACCACG AGAGCCTGTT GAGCAACTTC 
GGTCACGCCA GGATGCGAAA TGAGGCGGCT GCCGTGGCGG CCACTGCAGC TGCTCTTGGT 
CAACCCAAGG ACTTGGGCGT TCAGCTGCCA AACAGCAACG CGCCTGGGCA ATCCCTGCTG 
GACACCTATC TGCAGTTCAT CACGGAGAAT ACATTCGGCA TGGGCATGTC CCAGGAGCAG 
GCCGCTGCAG CAGCACTGCG CGCCAAGATG GCCCAGCTTA ACGCGATGGG ACACAGTCTG 
GACAACCTGC CGCCGGGCCT TCTTCCCGGA CAATTCGACC TGAGCAAGCT GGCCGCCGGA 
AATCCCGCCT TTGGACAGAG CGGACCCGGA CTAACCATTG AGCCGATAAT GCGACACGAA 
CAGGCAGCGG GAAACCTCTC GCCGAACCGA CCACTGGCTC TCAACTCGGG CGGCAGGATG 
ATGGGTCACG ATGAAATGGC GGAAAATGAT GGCGACATGC GGCGGGAAGG TTCGGAACCC 
ATGGATCTGG GCCTGGACAA CAACCAGTCG GGCAGCAACC ATGAGGTGGC CAACTCCGAT 
GCCGAGGAGA ACTACTCGGA GGATGAGGGA GTGCACAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162952.1
CDS	758..3442
Translation	MTESTQLQTAENNNAGVVKMEPPPPATSSVSVSAAAAAHALSSLSSLTMAATGSALSPATPPPSLNLSHQQQQHQQHYALKWNDFQSSILSSFRHLRDEEDFVDVTLACDERSFTAHKVVLSACSPYFRRLLKANPCEHPIVILRDVRCDDVENLLSFMYNGEVNVSHEQLPDFLKTAHLLQIRGLADVNGGYPYSKALSAALSHNSSNNNNNNSSSNNSLSNNNNNNNNNAESSNHNKISSYLSPNQTSAACNNSSNSNSNNHSSSHNNSSSNNISGSLNSSLNSPFSAPQIPPPVTASSAAAAAAAAASLTAAVAAAAAATAASAGSSSSAASGQTSGTPAIQELKASSAASPASSSNHWDMGEMEGSRKSHLTPPPQKRIKSADLFRAQHGISPERLLLDREFPVAGQHPLTRNRSGRDTSKDRERNLELRESLLGQALENSNGQQANPKHELGQSAGEDSNSSDTEPSDRGDGQHDGTLDGIDNQRSHSFPNAFLGLQGIPGLLPGPSGINSDFVSRRSLEMRVRATDPRPCPKCGKIYRSAHTLRTHLEDKHTVCPGYRCVLCGTVAKSRNSLHSHMSRQHRGISTKDLPVLPMPSAFDPELASRLLAKAGVKISPAELRARASPTGGSGSSGGGGGGGSSQAKLDLSNASGGPMDDAEDSDDDPEDLTTGNGLYGMGGSSSDLSRYHESLLSNFGHARMRNEAAAVAATAAALGQPKDLGVQLPNSNAPGQSLLDTYLQFITENTFGMGMSQEQAAAAALRAKMAQLNAMGHSLDNLPPGLLPGQFDLSKLAAGNPAFGQSGPGLTIEPIMRHEQAAGNLSPNRPLALNSGGRMMGHDEMAENDGDMRREGSEPMDLGLDNNQSGSNHEVANSDAEENYSEDEGVHNT

Target ORF information:

RefSeq Version	NM_001169481.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster abrupt (ab), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169481.2

ORF Insert Sequence:

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGCCAG CAGCAGCAAC 
CACTGGGACA TGGGCGAGAT GGAGGGCAGC CGGAAGAGCC ATCTGACGCC GCCGCCACAG 
AAACGCATCA AGAGCGCCGA CTTGTTCCGT GCCCAGCATG GCATCAGTCC CGAGCGATTG 
CTGTTGGATC GGGAGTTCCC CGTCGCCGGA CAGCATCCAC TCACAAGGAA CAGAAGCGGT 
CGGGACACAT CCAAGGATCG GGAGCGCAAC TTGGAGTTGA GGGAATCGCT ACTGGGACAG 
GCTTTGGAGA ACAGCAACGG CCAGCAGGCC AATCCGAAAC ACGAACTGGG ACAGAGCGCG 
GGTGAAGATT CGAACAGCAG TGATACCGAG CCCTCGGATC GCGGCGATGG TCAGCACGAT 
GGAACCCTCG ACGGGATTGA CAACCAGCGC TCGCACTCGT TCCCCAATGC ATTCCTCGGC 
CTCCAGGGCA TTCCAGGACT TCTGCCAGGA CCCTCTGGCA TCAACAGCGA TTTCGTTTCC 
CGACGCTCCC TGGAAATGCG AGTCCGAGCC ACAGATCCGC GTCCCTGCCC CAAGTGCGGA 
AAAATCTACC GCTCCGCCCA CACGCTACGC ACCCATCTGG AGGACAAGCA CACCGTGTGC 
CCCGGCTATC GATGTGTGCT CTGCGGCACG GTGGCCAAGT CCCGCAACTC TTTGCACTCG 
CATATGTCGC GCCAACATCG CGGCATCTCC ACCAAGGACC TGCCCGTCCT GCCCATGCCC 
AGTGCTTTCG ATCCAGAGCT CGCCTCGCGC CTTCTGGCCA AGGCGGGCGT CAAGATCTCA 
CCCGCTGAGC TGAGGGCCAG AGCCTCGCCC ACCGGCGGAA GTGGCAGCAG CGGCGGAGGC 
GGCGGAGGTG GTAGCAGCCA GGCCAAGTTG GATTTAAGCA ACGCTAGCGG TGGACCCATG 
GACGATGCCG AGGACTCGGA CGACGATCCC GAGGACCTGA CCACGGGCAA CGGATTGTAT 
GGAATGGGCG GAAGCAGCAG CGACCTGAGC CGTTACCACG AGAGCCTGTT GAGCAACTTC 
GGTCACGCCA GGATGCGAAA TGAGGCGGCT GCCGTGGCGG CCACTGCAGC TGCTCTTGGT 
CAACCCAAGG ACTTGGGCGT TCAGCTGCCA AACAGCAACG CGCCTGGGCA ATCCCTGCTG 
GACACCTATC TGCAGTTCAT CACGGAGAAT ACATTCGGCA TGGGCATGTC CCAGGAGCAG 
GCCGCTGCAG CAGCACTGCG CGCCAAGATG GCCCAGCTTA ACGCGATGGG ACACAGTCTG 
GACAACCTGC CGCCGGGCCT TCTTCCCGGA CAATTCGACC TGAGCAAGCT GGCCGCCGGA 
AATCCCGCCT TTGGACAGAG CGGACCCGGA CTAACCATTG AGCCGATAAT GCGACACGAA 
CAGGCAGCGG GAAACCTCTC GCCGAACCGA CCACTGGCTC TCAACTCGGG CGGCAGGATG 
ATGGGTCACG ATGAAATGGC GGAAAATGAT GGCGACATGC GGCGGGAAGG TTCGGAACCC 
ATGGATCTGG GCCTGGACAA CAACCAGTCG GGCAGCAACC ATGAGGTGGC CAACTCCGAT 
GCCGAGGAGA ACTACTCGGA GGATGAGGGA GTGCACAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ab ( NM_001273450.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260379.1 Drosophila melanogaster abrupt (ab), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa14456
Clone ID Related Accession (Same CDS sequence)	NM_001169481.2 , NM_001273450.1 , NM_057215.5
Accession Version	NM_001273450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	abrupt, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGCCAG CAGCAGCAAC 
CACTGGGACA TGGGCGAGAT GGAGGGCAGC CGGAAGAGCC ATCTGACGCC GCCGCCACAG 
AAACGCATCA AGAGCGCCGA CTTGTTCCGT GCCCAGCATG GCATCAGTCC CGAGCGATTG 
CTGTTGGATC GGGAGTTCCC CGTCGCCGGA CAGCATCCAC TCACAAGGAA CAGAAGCGGT 
CGGGACACAT CCAAGGATCG GGAGCGCAAC TTGGAGTTGA GGGAATCGCT ACTGGGACAG 
GCTTTGGAGA ACAGCAACGG CCAGCAGGCC AATCCGAAAC ACGAACTGGG ACAGAGCGCG 
GGTGAAGATT CGAACAGCAG TGATACCGAG CCCTCGGATC GCGGCGATGG TCAGCACGAT 
GGAACCCTCG ACGGGATTGA CAACCAGCGC TCGCACTCGT TCCCCAATGC ATTCCTCGGC 
CTCCAGGGCA TTCCAGGACT TCTGCCAGGA CCCTCTGGCA TCAACAGCGA TTTCGTTTCC 
CGACGCTCCC TGGAAATGCG AGTCCGAGCC ACAGATCCGC GTCCCTGCCC CAAGTGCGGA 
AAAATCTACC GCTCCGCCCA CACGCTACGC ACCCATCTGG AGGACAAGCA CACCGTGTGC 
CCCGGCTATC GATGTGTGCT CTGCGGCACG GTGGCCAAGT CCCGCAACTC TTTGCACTCG 
CATATGTCGC GCCAACATCG CGGCATCTCC ACCAAGGACC TGCCCGTCCT GCCCATGCCC 
AGTGCTTTCG ATCCAGAGCT CGCCTCGCGC CTTCTGGCCA AGGCGGGCGT CAAGATCTCA 
CCCGCTGAGC TGAGGGCCAG AGCCTCGCCC ACCGGCGGAA GTGGCAGCAG CGGCGGAGGC 
GGCGGAGGTG GTAGCAGCCA GGCCAAGTTG GATTTAAGCA ACGCTAGCGG TGGACCCATG 
GACGATGCCG AGGACTCGGA CGACGATCCC GAGGACCTGA CCACGGGCAA CGGATTGTAT 
GGAATGGGCG GAAGCAGCAG CGACCTGAGC CGTTACCACG AGAGCCTGTT GAGCAACTTC 
GGTCACGCCA GGATGCGAAA TGAGGCGGCT GCCGTGGCGG CCACTGCAGC TGCTCTTGGT 
CAACCCAAGG ACTTGGGCGT TCAGCTGCCA AACAGCAACG CGCCTGGGCA ATCCCTGCTG 
GACACCTATC TGCAGTTCAT CACGGAGAAT ACATTCGGCA TGGGCATGTC CCAGGAGCAG 
GCCGCTGCAG CAGCACTGCG CGCCAAGATG GCCCAGCTTA ACGCGATGGG ACACAGTCTG 
GACAACCTGC CGCCGGGCCT TCTTCCCGGA CAATTCGACC TGAGCAAGCT GGCCGCCGGA 
AATCCCGCCT TTGGACAGAG CGGACCCGGA CTAACCATTG AGCCGATAAT GCGACACGAA 
CAGGCAGCGG GAAACCTCTC GCCGAACCGA CCACTGGCTC TCAACTCGGG CGGCAGGATG 
ATGGGTCACG ATGAAATGGC GGAAAATGAT GGCGACATGC GGCGGGAAGG TTCGGAACCC 
ATGGATCTGG GCCTGGACAA CAACCAGTCG GGCAGCAACC ATGAGGTGGC CAACTCCGAT 
GCCGAGGAGA ACTACTCGGA GGATGAGGGA GTGCACAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260379.1
CDS	408..3092
Translation	MTESTQLQTAENNNAGVVKMEPPPPATSSVSVSAAAAAHALSSLSSLTMAATGSALSPATPPPSLNLSHQQQQHQQHYALKWNDFQSSILSSFRHLRDEEDFVDVTLACDERSFTAHKVVLSACSPYFRRLLKANPCEHPIVILRDVRCDDVENLLSFMYNGEVNVSHEQLPDFLKTAHLLQIRGLADVNGGYPYSKALSAALSHNSSNNNNNNSSSNNSLSNNNNNNNNNAESSNHNKISSYLSPNQTSAACNNSSNSNSNNHSSSHNNSSSNNISGSLNSSLNSPFSAPQIPPPVTASSAAAAAAAAASLTAAVAAAAAATAASAGSSSSAASGQTSGTPAIQELKASSAASPASSSNHWDMGEMEGSRKSHLTPPPQKRIKSADLFRAQHGISPERLLLDREFPVAGQHPLTRNRSGRDTSKDRERNLELRESLLGQALENSNGQQANPKHELGQSAGEDSNSSDTEPSDRGDGQHDGTLDGIDNQRSHSFPNAFLGLQGIPGLLPGPSGINSDFVSRRSLEMRVRATDPRPCPKCGKIYRSAHTLRTHLEDKHTVCPGYRCVLCGTVAKSRNSLHSHMSRQHRGISTKDLPVLPMPSAFDPELASRLLAKAGVKISPAELRARASPTGGSGSSGGGGGGGSSQAKLDLSNASGGPMDDAEDSDDDPEDLTTGNGLYGMGGSSSDLSRYHESLLSNFGHARMRNEAAAVAATAAALGQPKDLGVQLPNSNAPGQSLLDTYLQFITENTFGMGMSQEQAAAAALRAKMAQLNAMGHSLDNLPPGLLPGQFDLSKLAAGNPAFGQSGPGLTIEPIMRHEQAAGNLSPNRPLALNSGGRMMGHDEMAENDGDMRREGSEPMDLGLDNNQSGSNHEVANSDAEENYSEDEGVHNT

Target ORF information:

RefSeq Version	NM_001273450.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster abrupt (ab), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273450.1

ORF Insert Sequence:

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGCCAG CAGCAGCAAC 
CACTGGGACA TGGGCGAGAT GGAGGGCAGC CGGAAGAGCC ATCTGACGCC GCCGCCACAG 
AAACGCATCA AGAGCGCCGA CTTGTTCCGT GCCCAGCATG GCATCAGTCC CGAGCGATTG 
CTGTTGGATC GGGAGTTCCC CGTCGCCGGA CAGCATCCAC TCACAAGGAA CAGAAGCGGT 
CGGGACACAT CCAAGGATCG GGAGCGCAAC TTGGAGTTGA GGGAATCGCT ACTGGGACAG 
GCTTTGGAGA ACAGCAACGG CCAGCAGGCC AATCCGAAAC ACGAACTGGG ACAGAGCGCG 
GGTGAAGATT CGAACAGCAG TGATACCGAG CCCTCGGATC GCGGCGATGG TCAGCACGAT 
GGAACCCTCG ACGGGATTGA CAACCAGCGC TCGCACTCGT TCCCCAATGC ATTCCTCGGC 
CTCCAGGGCA TTCCAGGACT TCTGCCAGGA CCCTCTGGCA TCAACAGCGA TTTCGTTTCC 
CGACGCTCCC TGGAAATGCG AGTCCGAGCC ACAGATCCGC GTCCCTGCCC CAAGTGCGGA 
AAAATCTACC GCTCCGCCCA CACGCTACGC ACCCATCTGG AGGACAAGCA CACCGTGTGC 
CCCGGCTATC GATGTGTGCT CTGCGGCACG GTGGCCAAGT CCCGCAACTC TTTGCACTCG 
CATATGTCGC GCCAACATCG CGGCATCTCC ACCAAGGACC TGCCCGTCCT GCCCATGCCC 
AGTGCTTTCG ATCCAGAGCT CGCCTCGCGC CTTCTGGCCA AGGCGGGCGT CAAGATCTCA 
CCCGCTGAGC TGAGGGCCAG AGCCTCGCCC ACCGGCGGAA GTGGCAGCAG CGGCGGAGGC 
GGCGGAGGTG GTAGCAGCCA GGCCAAGTTG GATTTAAGCA ACGCTAGCGG TGGACCCATG 
GACGATGCCG AGGACTCGGA CGACGATCCC GAGGACCTGA CCACGGGCAA CGGATTGTAT 
GGAATGGGCG GAAGCAGCAG CGACCTGAGC CGTTACCACG AGAGCCTGTT GAGCAACTTC 
GGTCACGCCA GGATGCGAAA TGAGGCGGCT GCCGTGGCGG CCACTGCAGC TGCTCTTGGT 
CAACCCAAGG ACTTGGGCGT TCAGCTGCCA AACAGCAACG CGCCTGGGCA ATCCCTGCTG 
GACACCTATC TGCAGTTCAT CACGGAGAAT ACATTCGGCA TGGGCATGTC CCAGGAGCAG 
GCCGCTGCAG CAGCACTGCG CGCCAAGATG GCCCAGCTTA ACGCGATGGG ACACAGTCTG 
GACAACCTGC CGCCGGGCCT TCTTCCCGGA CAATTCGACC TGAGCAAGCT GGCCGCCGGA 
AATCCCGCCT TTGGACAGAG CGGACCCGGA CTAACCATTG AGCCGATAAT GCGACACGAA 
CAGGCAGCGG GAAACCTCTC GCCGAACCGA CCACTGGCTC TCAACTCGGG CGGCAGGATG 
ATGGGTCACG ATGAAATGGC GGAAAATGAT GGCGACATGC GGCGGGAAGG TTCGGAACCC 
ATGGATCTGG GCCTGGACAA CAACCAGTCG GGCAGCAACC ATGAGGTGGC CAACTCCGAT 
GCCGAGGAGA ACTACTCGGA GGATGAGGGA GTGCACAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ab ( NM_057215.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476563.1 Drosophila melanogaster abrupt (ab), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa14456
Clone ID Related Accession (Same CDS sequence)	NM_001169481.2 , NM_001273450.1 , NM_057215.5
Accession Version	NM_057215.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	abrupt, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057215.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGCCAG CAGCAGCAAC 
CACTGGGACA TGGGCGAGAT GGAGGGCAGC CGGAAGAGCC ATCTGACGCC GCCGCCACAG 
AAACGCATCA AGAGCGCCGA CTTGTTCCGT GCCCAGCATG GCATCAGTCC CGAGCGATTG 
CTGTTGGATC GGGAGTTCCC CGTCGCCGGA CAGCATCCAC TCACAAGGAA CAGAAGCGGT 
CGGGACACAT CCAAGGATCG GGAGCGCAAC TTGGAGTTGA GGGAATCGCT ACTGGGACAG 
GCTTTGGAGA ACAGCAACGG CCAGCAGGCC AATCCGAAAC ACGAACTGGG ACAGAGCGCG 
GGTGAAGATT CGAACAGCAG TGATACCGAG CCCTCGGATC GCGGCGATGG TCAGCACGAT 
GGAACCCTCG ACGGGATTGA CAACCAGCGC TCGCACTCGT TCCCCAATGC ATTCCTCGGC 
CTCCAGGGCA TTCCAGGACT TCTGCCAGGA CCCTCTGGCA TCAACAGCGA TTTCGTTTCC 
CGACGCTCCC TGGAAATGCG AGTCCGAGCC ACAGATCCGC GTCCCTGCCC CAAGTGCGGA 
AAAATCTACC GCTCCGCCCA CACGCTACGC ACCCATCTGG AGGACAAGCA CACCGTGTGC 
CCCGGCTATC GATGTGTGCT CTGCGGCACG GTGGCCAAGT CCCGCAACTC TTTGCACTCG 
CATATGTCGC GCCAACATCG CGGCATCTCC ACCAAGGACC TGCCCGTCCT GCCCATGCCC 
AGTGCTTTCG ATCCAGAGCT CGCCTCGCGC CTTCTGGCCA AGGCGGGCGT CAAGATCTCA 
CCCGCTGAGC TGAGGGCCAG AGCCTCGCCC ACCGGCGGAA GTGGCAGCAG CGGCGGAGGC 
GGCGGAGGTG GTAGCAGCCA GGCCAAGTTG GATTTAAGCA ACGCTAGCGG TGGACCCATG 
GACGATGCCG AGGACTCGGA CGACGATCCC GAGGACCTGA CCACGGGCAA CGGATTGTAT 
GGAATGGGCG GAAGCAGCAG CGACCTGAGC CGTTACCACG AGAGCCTGTT GAGCAACTTC 
GGTCACGCCA GGATGCGAAA TGAGGCGGCT GCCGTGGCGG CCACTGCAGC TGCTCTTGGT 
CAACCCAAGG ACTTGGGCGT TCAGCTGCCA AACAGCAACG CGCCTGGGCA ATCCCTGCTG 
GACACCTATC TGCAGTTCAT CACGGAGAAT ACATTCGGCA TGGGCATGTC CCAGGAGCAG 
GCCGCTGCAG CAGCACTGCG CGCCAAGATG GCCCAGCTTA ACGCGATGGG ACACAGTCTG 
GACAACCTGC CGCCGGGCCT TCTTCCCGGA CAATTCGACC TGAGCAAGCT GGCCGCCGGA 
AATCCCGCCT TTGGACAGAG CGGACCCGGA CTAACCATTG AGCCGATAAT GCGACACGAA 
CAGGCAGCGG GAAACCTCTC GCCGAACCGA CCACTGGCTC TCAACTCGGG CGGCAGGATG 
ATGGGTCACG ATGAAATGGC GGAAAATGAT GGCGACATGC GGCGGGAAGG TTCGGAACCC 
ATGGATCTGG GCCTGGACAA CAACCAGTCG GGCAGCAACC ATGAGGTGGC CAACTCCGAT 
GCCGAGGAGA ACTACTCGGA GGATGAGGGA GTGCACAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476563.1
CDS	806..3490
Translation	MTESTQLQTAENNNAGVVKMEPPPPATSSVSVSAAAAAHALSSLSSLTMAATGSALSPATPPPSLNLSHQQQQHQQHYALKWNDFQSSILSSFRHLRDEEDFVDVTLACDERSFTAHKVVLSACSPYFRRLLKANPCEHPIVILRDVRCDDVENLLSFMYNGEVNVSHEQLPDFLKTAHLLQIRGLADVNGGYPYSKALSAALSHNSSNNNNNNSSSNNSLSNNNNNNNNNAESSNHNKISSYLSPNQTSAACNNSSNSNSNNHSSSHNNSSSNNISGSLNSSLNSPFSAPQIPPPVTASSAAAAAAAAASLTAAVAAAAAATAASAGSSSSAASGQTSGTPAIQELKASSAASPASSSNHWDMGEMEGSRKSHLTPPPQKRIKSADLFRAQHGISPERLLLDREFPVAGQHPLTRNRSGRDTSKDRERNLELRESLLGQALENSNGQQANPKHELGQSAGEDSNSSDTEPSDRGDGQHDGTLDGIDNQRSHSFPNAFLGLQGIPGLLPGPSGINSDFVSRRSLEMRVRATDPRPCPKCGKIYRSAHTLRTHLEDKHTVCPGYRCVLCGTVAKSRNSLHSHMSRQHRGISTKDLPVLPMPSAFDPELASRLLAKAGVKISPAELRARASPTGGSGSSGGGGGGGSSQAKLDLSNASGGPMDDAEDSDDDPEDLTTGNGLYGMGGSSSDLSRYHESLLSNFGHARMRNEAAAVAATAAALGQPKDLGVQLPNSNAPGQSLLDTYLQFITENTFGMGMSQEQAAAAALRAKMAQLNAMGHSLDNLPPGLLPGQFDLSKLAAGNPAFGQSGPGLTIEPIMRHEQAAGNLSPNRPLALNSGGRMMGHDEMAENDGDMRREGSEPMDLGLDNNQSGSNHEVANSDAEENYSEDEGVHNT

Target ORF information:

RefSeq Version	NM_057215.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster abrupt (ab), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057215.5

ORF Insert Sequence:

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGCCAG CAGCAGCAAC 
CACTGGGACA TGGGCGAGAT GGAGGGCAGC CGGAAGAGCC ATCTGACGCC GCCGCCACAG 
AAACGCATCA AGAGCGCCGA CTTGTTCCGT GCCCAGCATG GCATCAGTCC CGAGCGATTG 
CTGTTGGATC GGGAGTTCCC CGTCGCCGGA CAGCATCCAC TCACAAGGAA CAGAAGCGGT 
CGGGACACAT CCAAGGATCG GGAGCGCAAC TTGGAGTTGA GGGAATCGCT ACTGGGACAG 
GCTTTGGAGA ACAGCAACGG CCAGCAGGCC AATCCGAAAC ACGAACTGGG ACAGAGCGCG 
GGTGAAGATT CGAACAGCAG TGATACCGAG CCCTCGGATC GCGGCGATGG TCAGCACGAT 
GGAACCCTCG ACGGGATTGA CAACCAGCGC TCGCACTCGT TCCCCAATGC ATTCCTCGGC 
CTCCAGGGCA TTCCAGGACT TCTGCCAGGA CCCTCTGGCA TCAACAGCGA TTTCGTTTCC 
CGACGCTCCC TGGAAATGCG AGTCCGAGCC ACAGATCCGC GTCCCTGCCC CAAGTGCGGA 
AAAATCTACC GCTCCGCCCA CACGCTACGC ACCCATCTGG AGGACAAGCA CACCGTGTGC 
CCCGGCTATC GATGTGTGCT CTGCGGCACG GTGGCCAAGT CCCGCAACTC TTTGCACTCG 
CATATGTCGC GCCAACATCG CGGCATCTCC ACCAAGGACC TGCCCGTCCT GCCCATGCCC 
AGTGCTTTCG ATCCAGAGCT CGCCTCGCGC CTTCTGGCCA AGGCGGGCGT CAAGATCTCA 
CCCGCTGAGC TGAGGGCCAG AGCCTCGCCC ACCGGCGGAA GTGGCAGCAG CGGCGGAGGC 
GGCGGAGGTG GTAGCAGCCA GGCCAAGTTG GATTTAAGCA ACGCTAGCGG TGGACCCATG 
GACGATGCCG AGGACTCGGA CGACGATCCC GAGGACCTGA CCACGGGCAA CGGATTGTAT 
GGAATGGGCG GAAGCAGCAG CGACCTGAGC CGTTACCACG AGAGCCTGTT GAGCAACTTC 
GGTCACGCCA GGATGCGAAA TGAGGCGGCT GCCGTGGCGG CCACTGCAGC TGCTCTTGGT 
CAACCCAAGG ACTTGGGCGT TCAGCTGCCA AACAGCAACG CGCCTGGGCA ATCCCTGCTG 
GACACCTATC TGCAGTTCAT CACGGAGAAT ACATTCGGCA TGGGCATGTC CCAGGAGCAG 
GCCGCTGCAG CAGCACTGCG CGCCAAGATG GCCCAGCTTA ACGCGATGGG ACACAGTCTG 
GACAACCTGC CGCCGGGCCT TCTTCCCGGA CAATTCGACC TGAGCAAGCT GGCCGCCGGA 
AATCCCGCCT TTGGACAGAG CGGACCCGGA CTAACCATTG AGCCGATAAT GCGACACGAA 
CAGGCAGCGG GAAACCTCTC GCCGAACCGA CCACTGGCTC TCAACTCGGG CGGCAGGATG 
ATGGGTCACG ATGAAATGGC GGAAAATGAT GGCGACATGC GGCGGGAAGG TTCGGAACCC 
ATGGATCTGG GCCTGGACAA CAACCAGTCG GGCAGCAACC ATGAGGTGGC CAACTCCGAT 
GCCGAGGAGA ACTACTCGGA GGATGAGGGA GTGCACAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ab ( NM_001273449.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260378.1 Drosophila melanogaster abrupt (ab), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa14457
Clone ID Related Accession (Same CDS sequence)	NM_001273449.1
Accession Version	NM_001273449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	abrupt, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGTAAG AAACCCGAAC 
CCCAATCCCA GCAAAGCCAG CAGCAGCAAC CACTGGGACA TGGGCGAGAT GGAGGGCAGC 
CGGAAGAGCC ATCTGACGCC GCCGCCACAG AAACGCATCA AGAGCGCCGA CTTGTTCCGT 
GCCCAGCATG GCATCAGTCC CGAGCGATTG CTGTTGGATC GGGAGTTCCC CGTCGCCGGA 
CAGCATCCAC TCACAAGGAA CAGAAGCGGT CGGGACACAT CCAAGGATCG GGAGCGCAAC 
TTGGAGTTGA GGGAATCGCT ACTGGGACAG GCTTTGGAGA ACAGCAACGG CCAGCAGGCC 
AATCCGAAAC ACGAACTGGG ACAGAGCGCG GGTGAAGATT CGAACAGCAG TGATACCGAG 
CCCTCGGATC GCGGCGATGG TCAGCACGAT GGAACCCTCG ACGGGATTGA CAACCAGCGC 
TCGCACTCGT TCCCCAATGC ATTCCTCGGC CTCCAGGGCA TTCCAGGACT TCTGCCAGGA 
CCCTCTGGCA TCAACAGCGA TTTCGGCACA TTCCTGAATG CCAAGAGCGG CATCAAACAG 
GAGGTGTTGG AGCCGGAAAC GGAAGAGGAT GCAGTGCAGC AACAGCGACC GCACGAGGCC 
CATGCCGCCG ACCGTCGTCC TGCGGCCTGT AAGCTGGCCA AGCTGATGCA GCAGCAGGAG 
CAGCAGCAGC GTCAACTGTT TGACCAGCGG CGCAGCCACC ACCAGCCTCA CCATCACCAC 
CAGCCGCATC ATCATCGCCA CCACCATCAT CACCAGCAAC GCATCAACAA CAATAACAAT 
AATACGAAAA AGAACCGATC GGCCAATTCC TCGCCCACGC CGTCGGCTCG ATCCTGGAAT 
GACAGCGAAG AGGATCAGGA CATGGACTGC AGCAGCGGCA ACCAGCAGCA GCATGGACAG 
CAGCAGCAAC AGCAGCACAA TAGCAACGAG GACGAGGATG ATCGCTCCTC GTCGGCCTCC 
TCGGCCATTT CCTTGACCAT GAAGAGAACG CGCCAGGATA GTGAGTCCAC GCTCTACCAA 
ACGCTACTCA TGAAGCAGGA GCAACACCAG CAGCAGCACC AGCAGCAACA CCAACAGCAG 
CAGCATCAGC AGCAGCAGCA GCAGTATCTG GACGAGCTGC AGCAGTCGCA CCAGCAGCAA 
CTGACGGCAA ATCTGTTTAT GGCCAGGACA ATAGCTCTAA GCAGGTCACG AGATTTTCCG 
GAACTCTTTC AAAACACCAT TGCCGCGGCG GCTGCGAATG CGACAGCTGC ATCGGCCAGT 
CCGGGGAACA ATGCCAGTGG ACCCGGTCAG GTGGTGGCAA CCGGACCCGG ACCGGGACCA 
GGAAGTGCTG CACCCGTGGC CGTGGGAAGT GGCAGCGGAG CGTCAGCCGG AAACAGCAAC 
TACATGCTGC CATGTCCGCT GTGTGAGACC CCACTGGAGC AGCGGGTATT CCGGCAGCAC 
CTGGACCGAC ACTATCCGCG CGACAGTCCC GTCTGTCCGG TGATCGAATG TGGACGCCGG 
TTCGCGCATC CCAATTCGGT GCGAAATCAC ATGCGCATCA AGCACACGCT GCAGTGGGCC 
AAGATGAAGG CAATGCGGTC GTCGGGCGGC CCCTTCGCCG GCGGTCCGGA CTTCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260378.1
CDS	681..3380
Translation	MTESTQLQTAENNNAGVVKMEPPPPATSSVSVSAAAAAHALSSLSSLTMAATGSALSPATPPPSLNLSHQQQQHQQHYALKWNDFQSSILSSFRHLRDEEDFVDVTLACDERSFTAHKVVLSACSPYFRRLLKANPCEHPIVILRDVRCDDVENLLSFMYNGEVNVSHEQLPDFLKTAHLLQIRGLADVNGGYPYSKALSAALSHNSSNNNNNNSSSNNSLSNNNNNNNNNAESSNHNKISSYLSPNQTSAACNNSSNSNSNNHSSSHNNSSSNNISGSLNSSLNSPFSAPQIPPPVTASSAAAAAAAAASLTAAVAAAAAATAASAGSSSSAASGQTSGTPAIQELKASSAASPVRNPNPNPSKASSSNHWDMGEMEGSRKSHLTPPPQKRIKSADLFRAQHGISPERLLLDREFPVAGQHPLTRNRSGRDTSKDRERNLELRESLLGQALENSNGQQANPKHELGQSAGEDSNSSDTEPSDRGDGQHDGTLDGIDNQRSHSFPNAFLGLQGIPGLLPGPSGINSDFGTFLNAKSGIKQEVLEPETEEDAVQQQRPHEAHAADRRPAACKLAKLMQQQEQQQRQLFDQRRSHHQPHHHHQPHHHRHHHHHQQRINNNNNNTKKNRSANSSPTPSARSWNDSEEDQDMDCSSGNQQQHGQQQQQQHNSNEDEDDRSSSASSAISLTMKRTRQDSESTLYQTLLMKQEQHQQQHQQQHQQQQHQQQQQQYLDELQQSHQQQLTANLFMARTIALSRSRDFPELFQNTIAAAAANATAASASPGNNASGPGQVVATGPGPGPGSAAPVAVGSGSGASAGNSNYMLPCPLCETPLEQRVFRQHLDRHYPRDSPVCPVIECGRRFAHPNSVRNHMRIKHTLQWAKMKAMRSSGGPFAGGPDFK

Target ORF information:

RefSeq Version	NM_001273449.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster abrupt (ab), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273449.1

ORF Insert Sequence:

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGTAAG AAACCCGAAC 
CCCAATCCCA GCAAAGCCAG CAGCAGCAAC CACTGGGACA TGGGCGAGAT GGAGGGCAGC 
CGGAAGAGCC ATCTGACGCC GCCGCCACAG AAACGCATCA AGAGCGCCGA CTTGTTCCGT 
GCCCAGCATG GCATCAGTCC CGAGCGATTG CTGTTGGATC GGGAGTTCCC CGTCGCCGGA 
CAGCATCCAC TCACAAGGAA CAGAAGCGGT CGGGACACAT CCAAGGATCG GGAGCGCAAC 
TTGGAGTTGA GGGAATCGCT ACTGGGACAG GCTTTGGAGA ACAGCAACGG CCAGCAGGCC 
AATCCGAAAC ACGAACTGGG ACAGAGCGCG GGTGAAGATT CGAACAGCAG TGATACCGAG 
CCCTCGGATC GCGGCGATGG TCAGCACGAT GGAACCCTCG ACGGGATTGA CAACCAGCGC 
TCGCACTCGT TCCCCAATGC ATTCCTCGGC CTCCAGGGCA TTCCAGGACT TCTGCCAGGA 
CCCTCTGGCA TCAACAGCGA TTTCGGCACA TTCCTGAATG CCAAGAGCGG CATCAAACAG 
GAGGTGTTGG AGCCGGAAAC GGAAGAGGAT GCAGTGCAGC AACAGCGACC GCACGAGGCC 
CATGCCGCCG ACCGTCGTCC TGCGGCCTGT AAGCTGGCCA AGCTGATGCA GCAGCAGGAG 
CAGCAGCAGC GTCAACTGTT TGACCAGCGG CGCAGCCACC ACCAGCCTCA CCATCACCAC 
CAGCCGCATC ATCATCGCCA CCACCATCAT CACCAGCAAC GCATCAACAA CAATAACAAT 
AATACGAAAA AGAACCGATC GGCCAATTCC TCGCCCACGC CGTCGGCTCG ATCCTGGAAT 
GACAGCGAAG AGGATCAGGA CATGGACTGC AGCAGCGGCA ACCAGCAGCA GCATGGACAG 
CAGCAGCAAC AGCAGCACAA TAGCAACGAG GACGAGGATG ATCGCTCCTC GTCGGCCTCC 
TCGGCCATTT CCTTGACCAT GAAGAGAACG CGCCAGGATA GTGAGTCCAC GCTCTACCAA 
ACGCTACTCA TGAAGCAGGA GCAACACCAG CAGCAGCACC AGCAGCAACA CCAACAGCAG 
CAGCATCAGC AGCAGCAGCA GCAGTATCTG GACGAGCTGC AGCAGTCGCA CCAGCAGCAA 
CTGACGGCAA ATCTGTTTAT GGCCAGGACA ATAGCTCTAA GCAGGTCACG AGATTTTCCG 
GAACTCTTTC AAAACACCAT TGCCGCGGCG GCTGCGAATG CGACAGCTGC ATCGGCCAGT 
CCGGGGAACA ATGCCAGTGG ACCCGGTCAG GTGGTGGCAA CCGGACCCGG ACCGGGACCA 
GGAAGTGCTG CACCCGTGGC CGTGGGAAGT GGCAGCGGAG CGTCAGCCGG AAACAGCAAC 
TACATGCTGC CATGTCCGCT GTGTGAGACC CCACTGGAGC AGCGGGTATT CCGGCAGCAC 
CTGGACCGAC ACTATCCGCG CGACAGTCCC GTCTGTCCGG TGATCGAATG TGGACGCCGG 
TTCGCGCATC CCAATTCGGT GCGAAATCAC ATGCGCATCA AGCACACGCT GCAGTGGGCC 
AAGATGAAGG CAATGCGGTC GTCGGGCGGC CCCTTCGCCG GCGGTCCGGA CTTCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ab ( NM_057214.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476562.1 Drosophila melanogaster abrupt (ab), transcript variant A, mRNA.

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CloneID	OFa16675
Clone ID Related Accession (Same CDS sequence)	NM_057214.5
Accession Version	NM_057214.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	abrupt, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057214.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGTAAG AAACCCGAAC 
CCCAATCCCA GCAAAGCCAG CAGCAGCAAC CACTGGGACA TGGGCGAGAT GGAGGGCAGC 
CGGAAGAGCC ATCTGACGCC GCCGCCACAG AAACGCATCA AGAGCGCCGA CTTGTTCCGT 
GCCCAGCATG GCATCAGTCC CGAGCGATTG CTGTTGGATC GGGAGTTCCC CGTCGCCGGA 
CAGCATCCAC TCACAAGGAA CAGAAGCGGT CGGGACACAT CCAAGGATCG GGAGCGCAAC 
TTGGAGTTGA GGGAATCGCT ACTGGGACAG GCTTTGGAGA ACAGCAACGG CCAGCAGGCC 
AATCCGAAAC ACGAACTGGG ACAGAGCGCG GGTGAAGATT CGAACAGCAG TGATACCGAG 
CCCTCGGATC GCGGCGATGG TCAGCACGAT GGAACCCTCG ACGGGATTGA CAACCAGCGC 
TCGCACTCGT TCCCCAATGC ATTCCTCGGC CTCCAGGGCA TTCCAGGACT TCTGCCAGGA 
CCCTCTGGCA TCAACAGCGA TTTCGTTTCC CGACGCTCCC TGGAAATGCG AGTCCGAGCC 
ACAGATCCGC GTCCCTGCCC CAAGTGCGGA AAAATCTACC GCTCCGCCCA CACGCTACGC 
ACCCATCTGG AGGACAAGCA CACCGTGTGC CCCGGCTATC GATGTGTGCT CTGCGGCACG 
GTGGCCAAGT CCCGCAACTC TTTGCACTCG CATATGTCGC GCCAACATCG CGGCATCTCC 
ACCAAGGACC TGCCCGTCCT GCCCATGCCC AGTGCTTTCG ATCCAGAGCT CGCCTCGCGC 
CTTCTGGCCA AGGCGGGCGT CAAGATCTCA CCCGCTGAGC TGAGGGCCAG AGCCTCGCCC 
ACCGGCGGAA GTGGCAGCAG CGGCGGAGGC GGCGGAGGTG GTAGCAGCCA GGCCAAGTTG 
GATTTAAGCA ACGCTAGCGG TGGACCCATG GACGATGCCG AGGACTCGGA CGACGATCCC 
GAGGACCTGA CCACGGGCAA CGGATTGTAT GGAATGGGCG GAAGCAGCAG CGACCTGAGC 
CGTTACCACG AGAGCCTGTT GAGCAACTTC GGTCACGCCA GGATGCGAAA TGAGGCGGCT 
GCCGTGGCGG CCACTGCAGC TGCTCTTGGT CAACCCAAGG ACTTGGGCGT TCAGCTGCCA 
AACAGCAACG CGCCTGGGCA ATCCCTGCTG GACACCTATC TGCAGTTCAT CACGGAGAAT 
ACATTCGGCA TGGGCATGTC CCAGGAGCAG GCCGCTGCAG CAGCACTGCG CGCCAAGATG 
GCCCAGCTTA ACGCGATGGG ACACAGTCTG GACAACCTGC CGCCGGGCCT TCTTCCCGGA 
CAATTCGACC TGAGCAAGCT GGCCGCCGGA AATCCCGCCT TTGGACAGAG CGGACCCGGA 
CTAACCATTG AGCCGATAAT GCGACACGAA CAGGCAGCGG GAAACCTCTC GCCGAACCGA 
CCACTGGCTC TCAACTCGGG CGGCAGGATG ATGGGTCACG ATGAAATGGC GGAAAATGAT 
GGCGACATGC GGCGGGAAGG TTCGGAACCC ATGGATCTGG GCCTGGACAA CAACCAGTCG 
GGCAGCAACC ATGAGGTGGC CAACTCCGAT GCCGAGGAGA ACTACTCGGA GGATGAGGGA 
GTGCACAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476562.1
CDS	780..3494
Translation	MTESTQLQTAENNNAGVVKMEPPPPATSSVSVSAAAAAHALSSLSSLTMAATGSALSPATPPPSLNLSHQQQQHQQHYALKWNDFQSSILSSFRHLRDEEDFVDVTLACDERSFTAHKVVLSACSPYFRRLLKANPCEHPIVILRDVRCDDVENLLSFMYNGEVNVSHEQLPDFLKTAHLLQIRGLADVNGGYPYSKALSAALSHNSSNNNNNNSSSNNSLSNNNNNNNNNAESSNHNKISSYLSPNQTSAACNNSSNSNSNNHSSSHNNSSSNNISGSLNSSLNSPFSAPQIPPPVTASSAAAAAAAAASLTAAVAAAAAATAASAGSSSSAASGQTSGTPAIQELKASSAASPVRNPNPNPSKASSSNHWDMGEMEGSRKSHLTPPPQKRIKSADLFRAQHGISPERLLLDREFPVAGQHPLTRNRSGRDTSKDRERNLELRESLLGQALENSNGQQANPKHELGQSAGEDSNSSDTEPSDRGDGQHDGTLDGIDNQRSHSFPNAFLGLQGIPGLLPGPSGINSDFVSRRSLEMRVRATDPRPCPKCGKIYRSAHTLRTHLEDKHTVCPGYRCVLCGTVAKSRNSLHSHMSRQHRGISTKDLPVLPMPSAFDPELASRLLAKAGVKISPAELRARASPTGGSGSSGGGGGGGSSQAKLDLSNASGGPMDDAEDSDDDPEDLTTGNGLYGMGGSSSDLSRYHESLLSNFGHARMRNEAAAVAATAAALGQPKDLGVQLPNSNAPGQSLLDTYLQFITENTFGMGMSQEQAAAAALRAKMAQLNAMGHSLDNLPPGLLPGQFDLSKLAAGNPAFGQSGPGLTIEPIMRHEQAAGNLSPNRPLALNSGGRMMGHDEMAENDGDMRREGSEPMDLGLDNNQSGSNHEVANSDAEENYSEDEGVHNT

Target ORF information:

RefSeq Version	NM_057214.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster abrupt (ab), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057214.5

ORF Insert Sequence:

ATGACCGAAT CCACACAGCT GCAGACGGCG GAGAACAACA ACGCGGGCGT GGTCAAAATG 
GAGCCCCCGC CGCCGGCGAC CTCCTCCGTT TCCGTATCCG CCGCCGCAGC CGCCCACGCC 
CTCTCCTCCC TCTCTTCGCT TACAATGGCC GCCACCGGAT CCGCCCTCTC GCCGGCCACG 
CCCCCGCCCT CCCTGAACCT CTCACATCAG CAGCAGCAGC ACCAGCAGCA CTACGCCCTC 
AAGTGGAATG ACTTCCAGAG CTCGATCCTC AGCTCCTTCC GTCACCTGCG GGACGAGGAG 
GATTTCGTCG ACGTGACGCT GGCCTGCGAC GAGCGTTCCT TCACCGCCCA CAAGGTCGTC 
CTGAGCGCCT GCAGCCCCTA CTTCCGCCGG CTGCTCAAGG CCAATCCCTG CGAGCACCCG 
ATAGTCATCC TGCGCGACGT GCGCTGCGAT GATGTCGAGA ATCTGCTGAG CTTTATGTAC 
AATGGTGAGG TGAATGTGAG CCACGAACAG TTGCCCGACT TTCTGAAGAC AGCTCACCTG 
CTGCAGATTC GCGGTCTGGC GGATGTGAAT GGTGGCTATC CCTATTCCAA GGCCTTGTCC 
GCCGCCTTGA GTCACAACAG CAGCAATAAC AACAACAACA ACAGTAGCAG CAACAACAGC 
CTGAGCAACA ACAATAACAA TAACAACAAT AATGCCGAGA GCAGTAATCA TAACAAGATA 
AGCAGCTATT TATCGCCCAA TCAAACGAGC GCCGCGTGCA ACAACAGCAG CAACAGCAAT 
AGCAACAACC ACAGCAGCAG CCACAACAAC AGCAGCAGCA ACAACATAAG CGGATCCCTG 
AACAGCAGCC TGAACTCACC GTTTAGTGCG CCACAGATAC CGCCACCAGT GACCGCATCG 
AGTGCAGCGG CAGCAGCAGC AGCGGCCGCA TCCCTGACCG CCGCAGTAGC CGCAGCAGCG 
GCAGCAACAG CAGCCAGTGC CGGCAGCAGC AGCAGCGCCG CCAGCGGACA GACGAGCGGT 
ACGCCCGCCA TCCAGGAGCT GAAGGCATCG TCGGCAGCGT CGCCCGTAAG AAACCCGAAC 
CCCAATCCCA GCAAAGCCAG CAGCAGCAAC CACTGGGACA TGGGCGAGAT GGAGGGCAGC 
CGGAAGAGCC ATCTGACGCC GCCGCCACAG AAACGCATCA AGAGCGCCGA CTTGTTCCGT 
GCCCAGCATG GCATCAGTCC CGAGCGATTG CTGTTGGATC GGGAGTTCCC CGTCGCCGGA 
CAGCATCCAC TCACAAGGAA CAGAAGCGGT CGGGACACAT CCAAGGATCG GGAGCGCAAC 
TTGGAGTTGA GGGAATCGCT ACTGGGACAG GCTTTGGAGA ACAGCAACGG CCAGCAGGCC 
AATCCGAAAC ACGAACTGGG ACAGAGCGCG GGTGAAGATT CGAACAGCAG TGATACCGAG 
CCCTCGGATC GCGGCGATGG TCAGCACGAT GGAACCCTCG ACGGGATTGA CAACCAGCGC 
TCGCACTCGT TCCCCAATGC ATTCCTCGGC CTCCAGGGCA TTCCAGGACT TCTGCCAGGA 
CCCTCTGGCA TCAACAGCGA TTTCGTTTCC CGACGCTCCC TGGAAATGCG AGTCCGAGCC 
ACAGATCCGC GTCCCTGCCC CAAGTGCGGA AAAATCTACC GCTCCGCCCA CACGCTACGC 
ACCCATCTGG AGGACAAGCA CACCGTGTGC CCCGGCTATC GATGTGTGCT CTGCGGCACG 
GTGGCCAAGT CCCGCAACTC TTTGCACTCG CATATGTCGC GCCAACATCG CGGCATCTCC 
ACCAAGGACC TGCCCGTCCT GCCCATGCCC AGTGCTTTCG ATCCAGAGCT CGCCTCGCGC 
CTTCTGGCCA AGGCGGGCGT CAAGATCTCA CCCGCTGAGC TGAGGGCCAG AGCCTCGCCC 
ACCGGCGGAA GTGGCAGCAG CGGCGGAGGC GGCGGAGGTG GTAGCAGCCA GGCCAAGTTG 
GATTTAAGCA ACGCTAGCGG TGGACCCATG GACGATGCCG AGGACTCGGA CGACGATCCC 
GAGGACCTGA CCACGGGCAA CGGATTGTAT GGAATGGGCG GAAGCAGCAG CGACCTGAGC 
CGTTACCACG AGAGCCTGTT GAGCAACTTC GGTCACGCCA GGATGCGAAA TGAGGCGGCT 
GCCGTGGCGG CCACTGCAGC TGCTCTTGGT CAACCCAAGG ACTTGGGCGT TCAGCTGCCA 
AACAGCAACG CGCCTGGGCA ATCCCTGCTG GACACCTATC TGCAGTTCAT CACGGAGAAT 
ACATTCGGCA TGGGCATGTC CCAGGAGCAG GCCGCTGCAG CAGCACTGCG CGCCAAGATG 
GCCCAGCTTA ACGCGATGGG ACACAGTCTG GACAACCTGC CGCCGGGCCT TCTTCCCGGA 
CAATTCGACC TGAGCAAGCT GGCCGCCGGA AATCCCGCCT TTGGACAGAG CGGACCCGGA 
CTAACCATTG AGCCGATAAT GCGACACGAA CAGGCAGCGG GAAACCTCTC GCCGAACCGA 
CCACTGGCTC TCAACTCGGG CGGCAGGATG ATGGGTCACG ATGAAATGGC GGAAAATGAT 
GGCGACATGC GGCGGGAAGG TTCGGAACCC ATGGATCTGG GCCTGGACAA CAACCAGTCG 
GGCAGCAACC ATGAGGTGGC CAACTCCGAT GCCGAGGAGA ACTACTCGGA GGATGAGGGA 
GTGCACAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ab cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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