gcm2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gcm2
Entrez Gene ID	34280
Full Name	CG3858 gene product from transcript CG3858-RA
Synonyms	CG3858,Dmel\CG3858,FBgn0019809,Gcm2,dGCM2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30C1-30C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_135458.5	NP_609302.1	gcm2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

gcm and gcm2 are expressed in precursors and postmitotic, migrating, cells of the medulla neuropile glia (mng) lineage, and also in a thoracic glial lineage and in neurons of the ventral nerve cord
Title: Novel gcm-dependent lineages in the postembryonic nervous system of Drosophila melanogaster.

Gcm and Gcm2 are expressed in both glial and neuronal precursors within the optic lobe and autonomously regulate both glial and neuronal development in the visual system.
Title: glial cells missing and gcm2 cell autonomously regulate both glial and neuronal development in the visual system of Drosophila.

gcm2 promotes glial cell differentiation and is required with glial cells missing for macrophage development in Drosophila
Title: gcm2 promotes glial cell differentiation and is required with glial cells missing for macrophage development in Drosophila.

The following gcm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gcm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14212	NM_135458.5 Latest version!	Drosophila melanogaster gcm2 (gcm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gcm2 ( NM_135458.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609302.1 Drosophila melanogaster gcm2 (gcm2), mRNA.

Price & Availability↑

CloneID	OFa14212
Clone ID Related Accession (Same CDS sequence)	NM_135458.5
Accession Version	NM_135458.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gcm2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135458.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTGTTA TAAATGGCTA CAGCTTCAAG ACGCAGCAAC TTCTCACACA GCAACCCGAT 
CATTCTCAGC TTACCCAGTT TGTCCAGCCC CAAAGTCAGA GTACGCATTC GGTGCATCCG 
GGTCCAAGTC CTGGTCAACA GCAGGCGGGT GGCTCCATGA CCATGCCGTC GTCGTCGACG 
GGCAAGGGAA AACGGGAGTG GGACATCAAT GATGCCATTG TGCCACATGT GCCGGACCAG 
GAGTTCGACG AGTTCAACGA GTGGAGCGAC GGGCATGTGA GGCACATCTA TTCGCTGCAC 
AACGAGGAGG CCAAGAAGCA CATTTCCGGC TGGGCCATGC GCAACACGAA CAACCACAAC 
GTGAACATTC TGAAGAAGAG CTGCCTGGGC GTCCTGGTCT GCTCCCAACA CTGCACCCTG 
CCCAACGGAT CCAAGATCAA CCTGCGCCCA GCCATTTGCG ACAAAGCCCG GCGGAAGCAG 
GAGGGCAAGG CGTGTCCCAA CAAAAGTTGT CGTGGCGGTA GACTAGAGAT TAAACCGTGT 
CGCGGTCACT GCGGCTATCC GGTGACCCAC TTCTGGCGGC ACTCCGGCAA TGCCATCTTT 
TTTCAGGCCA AGGGAGTGCA TGATCACCTG CGTCCGGATC CGAAGAACTC CAGTGTTTCC 
AAGCGGGCCT TTGGACGTGT GCCGCTGGCT GGAAAGTCAG CCAACGGATC AGTCGCCAAG 
AAGTCGGTCA TTGCGGGTCT GGTCAAGCAG GCGAAGCAAC AACACAGTCT GATTAGCAAG 
GTTCTTAAGC GTCCTGCTGT CAGCAATCCT CTGGCTCACA CCGCATTGGA TATATATCAG 
TATAATGCCT GTGGCAAGTG CGCCGGTTAT AGCCACTGCA CTTGCAGCTA CCTGGATGAC 
AGCACCACGG CGAGGAGCCA TCAGTTGTCA CAATCCTCGA ATTACGGCAC CAATTCTTGG 
CCACTAAGTG GATCCGAGTC ATCGGCACCC TGCGAGACGG CAGCTAATGT GTTTACGGTC 
AACCACCAGC ACATCACCTA TAACTATCCC ATCTACCATG CAACTCCAGC GGCGGCCACC 
GCGGCACCTA GTAAATCTCC CAGTTTGCCC TACGCCTGCA GCATTTCGGA ACTGGCCGCA 
TACCAGCAAT CCTCCAGCGG GAACAGCTTT GCGATGGGTG TTCCCGTGCA CGGACACACA 
CAATGCCAGG CAGTAGCCTA TGATTCCAGC CCGCAACTGG CCACTCCGGA ACCGGAGTTC 
ATCAACTACT CGCAGATCAA GCATTTGGGT GGTGGCGGTG GTCAGGAGGA GATCAGCTGC 
AAGGCCGAAC CGGGACCCAC CATTAAGTAC AATGCCACCG TGGAGACGCA GCCGTATGTG 
GAGGACAACT ACGATTACTA CTATAGCCCC AAGGCGGAGT ACGAAATGCA ACAGCACCAC 
CACCAGCAGC AGCAGAGCCA CCAACAGTTC GGTGGAAATC AGACAGCGGG ACATCACTAC 
TACGAGAGCA GTAGTGGCTA TAATGGAGTC AGCTATTTCG ACACAGGAAC TACTACGGCA 
CCAGGAAATA CTGCAACCGG AAATGGCCTG GAAGTCGGAT ACGGCGGCTA CTACGATCAC 
TACACCAGCT ACGAGCAACA AATGGCGGTA GCAGGTGGAT TTGCCACTGC CGGAGGATCC 
ACTGCGCCGA CAGTGGCCGC GCCCCCTGGC CATCCACCGC CTCCACCACC ACCGCCCACG 
CTGACGTACC ACCATCACCA TCACCACCAC TTGCACCACC CAGCAGCAGC TACAGGCCTA 
GCCCCATCGG TCACCCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609302.1
CDS	337..2157
Translation	MVVINGYSFKTQQLLTQQPDHSQLTQFVQPQSQSTHSVHPGPSPGQQQAGGSMTMPSSSTGKGKREWDINDAIVPHVPDQEFDEFNEWSDGHVRHIYSLHNEEAKKHISGWAMRNTNNHNVNILKKSCLGVLVCSQHCTLPNGSKINLRPAICDKARRKQEGKACPNKSCRGGRLEIKPCRGHCGYPVTHFWRHSGNAIFFQAKGVHDHLRPDPKNSSVSKRAFGRVPLAGKSANGSVAKKSVIAGLVKQAKQQHSLISKVLKRPAVSNPLAHTALDIYQYNACGKCAGYSHCTCSYLDDSTTARSHQLSQSSNYGTNSWPLSGSESSAPCETAANVFTVNHQHITYNYPIYHATPAAATAAPSKSPSLPYACSISELAAYQQSSSGNSFAMGVPVHGHTQCQAVAYDSSPQLATPEPEFINYSQIKHLGGGGGQEEISCKAEPGPTIKYNATVETQPYVEDNYDYYYSPKAEYEMQQHHHQQQQSHQQFGGNQTAGHHYYESSSGYNGVSYFDTGTTTAPGNTATGNGLEVGYGGYYDHYTSYEQQMAVAGGFATAGGSTAPTVAAPPGHPPPPPPPPTLTYHHHHHHHLHHPAAATGLAPSVTH

Target ORF information:

RefSeq Version	NM_135458.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gcm2 (gcm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135458.5

ORF Insert Sequence:

ATGGTTGTTA TAAATGGCTA CAGCTTCAAG ACGCAGCAAC TTCTCACACA GCAACCCGAT 
CATTCTCAGC TTACCCAGTT TGTCCAGCCC CAAAGTCAGA GTACGCATTC GGTGCATCCG 
GGTCCAAGTC CTGGTCAACA GCAGGCGGGT GGCTCCATGA CCATGCCGTC GTCGTCGACG 
GGCAAGGGAA AACGGGAGTG GGACATCAAT GATGCCATTG TGCCACATGT GCCGGACCAG 
GAGTTCGACG AGTTCAACGA GTGGAGCGAC GGGCATGTGA GGCACATCTA TTCGCTGCAC 
AACGAGGAGG CCAAGAAGCA CATTTCCGGC TGGGCCATGC GCAACACGAA CAACCACAAC 
GTGAACATTC TGAAGAAGAG CTGCCTGGGC GTCCTGGTCT GCTCCCAACA CTGCACCCTG 
CCCAACGGAT CCAAGATCAA CCTGCGCCCA GCCATTTGCG ACAAAGCCCG GCGGAAGCAG 
GAGGGCAAGG CGTGTCCCAA CAAAAGTTGT CGTGGCGGTA GACTAGAGAT TAAACCGTGT 
CGCGGTCACT GCGGCTATCC GGTGACCCAC TTCTGGCGGC ACTCCGGCAA TGCCATCTTT 
TTTCAGGCCA AGGGAGTGCA TGATCACCTG CGTCCGGATC CGAAGAACTC CAGTGTTTCC 
AAGCGGGCCT TTGGACGTGT GCCGCTGGCT GGAAAGTCAG CCAACGGATC AGTCGCCAAG 
AAGTCGGTCA TTGCGGGTCT GGTCAAGCAG GCGAAGCAAC AACACAGTCT GATTAGCAAG 
GTTCTTAAGC GTCCTGCTGT CAGCAATCCT CTGGCTCACA CCGCATTGGA TATATATCAG 
TATAATGCCT GTGGCAAGTG CGCCGGTTAT AGCCACTGCA CTTGCAGCTA CCTGGATGAC 
AGCACCACGG CGAGGAGCCA TCAGTTGTCA CAATCCTCGA ATTACGGCAC CAATTCTTGG 
CCACTAAGTG GATCCGAGTC ATCGGCACCC TGCGAGACGG CAGCTAATGT GTTTACGGTC 
AACCACCAGC ACATCACCTA TAACTATCCC ATCTACCATG CAACTCCAGC GGCGGCCACC 
GCGGCACCTA GTAAATCTCC CAGTTTGCCC TACGCCTGCA GCATTTCGGA ACTGGCCGCA 
TACCAGCAAT CCTCCAGCGG GAACAGCTTT GCGATGGGTG TTCCCGTGCA CGGACACACA 
CAATGCCAGG CAGTAGCCTA TGATTCCAGC CCGCAACTGG CCACTCCGGA ACCGGAGTTC 
ATCAACTACT CGCAGATCAA GCATTTGGGT GGTGGCGGTG GTCAGGAGGA GATCAGCTGC 
AAGGCCGAAC CGGGACCCAC CATTAAGTAC AATGCCACCG TGGAGACGCA GCCGTATGTG 
GAGGACAACT ACGATTACTA CTATAGCCCC AAGGCGGAGT ACGAAATGCA ACAGCACCAC 
CACCAGCAGC AGCAGAGCCA CCAACAGTTC GGTGGAAATC AGACAGCGGG ACATCACTAC 
TACGAGAGCA GTAGTGGCTA TAATGGAGTC AGCTATTTCG ACACAGGAAC TACTACGGCA 
CCAGGAAATA CTGCAACCGG AAATGGCCTG GAAGTCGGAT ACGGCGGCTA CTACGATCAC 
TACACCAGCT ACGAGCAACA AATGGCGGTA GCAGGTGGAT TTGCCACTGC CGGAGGATCC 
ACTGCGCCGA CAGTGGCCGC GCCCCCTGGC CATCCACCGC CTCCACCACC ACCGCCCACG 
CTGACGTACC ACCATCACCA TCACCACCAC TTGCACCACC CAGCAGCAGC TACAGGCCTA 
GCCCCATCGG TCACCCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gcm2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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