CG7154 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG7154
Entrez Gene ID	34062
Full Name	CG7154 gene product from transcript CG7154-RA
Synonyms	BRD7,Dmel\CG7154
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28C3-28C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_135304.3	NP_609148.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG7154 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG7154 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16401	NM_135304.3 Latest version!	Drosophila melanogaster uncharacterized protein (CG7154), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG7154 ( NM_135304.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609148.1 Drosophila melanogaster uncharacterized protein (CG7154), mRNA.

Price & Availability↑

CloneID	OFa16401
Clone ID Related Accession (Same CDS sequence)	NM_135304.3
Accession Version	NM_135304.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135304.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCTCCT CCAAGAAGCA CAAGAAAAAC AAGTCGGAGC GACGCGAGAA ATATGAAGAA 
TACAGTCAGC ACCAGGACCC GGCTCAGCTG CAACGAGGTC TAAAGCTTAT CCTTAAGGTG 
GGCTCGAATG CCACGCCGGA GTACAGTGCC AATTCGCCAA TGGTGGACGG AGGACCGCCC 
ACAGCAGCCG AGGCCATGAT GTCGCCAGTC CCCGAGGAAC TTCAAGATCA CCAGGGTCAT 
CGCGAGCGCC ACAAAAAGTC AAAGAAAAAA AAGAAGAAGA AGGATCGCGA GAAGAAGCAC 
AAACACCACA AGGAGAAACG GCACAGGAGC AGAGATAGAC ATCGGGACGC TGGATCGGAC 
GAAGATATGA TGGCCGGCGC TGACGATGCC GCTTGCAGTG GATTCGCCCC ATCGTCTGTC 
GCTCCACCGG CCGCCGATCC CGATTCCAGC CAAGATGGAT TTAGCTTTAT GGACGATGAT 
CAATCACAAC CGCTGCCAGA GAATATTTTG TTCTTCGCCG GCATCACCAC GGATAACTCG 
CCATCAAACT GCCCCGTAAC AAAGCCAATA GCTCCACGCA AGCTGGATGA TATTCTGATG 
GGCTCTTCGC CCAACTCGTC GTCGTTGCAA TCTTCCTCCC TGGGTTTAAT AGGCAGCAGT 
CCCACCAAGC CACTGCCCGA CCTTTTAATA CCTTCACCGT CGACGCCAGG AGGAGCCAAC 
TCCCTTAATG CCCTTACACC GAAGGCTCTC GAGGCGCCAA AGACGCCCAG TAGTTCCAGT 
GAGTCGGGTA GAGAGCCACG GAGCTGTGTG CTCAAGCTAA AGCAGCAGAA ATCGCCGTTA 
AACAAACTTC TGGAGCACCT GTTGCGCTTC CTGGAGAAGC GGGATCCCCA TCAGTTCTTC 
GCTTGGCCGG TGACCGATGA CATGGCGCCC GGTTACTCTT CAATAATCAG CAGACCCATG 
GACTTCTCCA CTATGCGACA GAAAATTGAT GATCACGAGT ATACCGCTTT AACCGAGTTC 
ACTGATGACT TTAAGCTGAT GTGCGAAAAT GCCATCAAGT ACAACCACGT GGATACGGTT 
TATAACAAGG CAGCTAAACG TCTACTCCAA GTTGGAATGA AACATCTGCA ACCTGAGAAC 
CTCATGAGAA GCCTGAAACC ACTATCTGGT TATATGAGGG AGCTTACAGC CAGAGAACTT 
GGCTTTGAGT TAAGTTCGAA CGATATGTCA AGGGAAAATA ACGATTCGGC GGATGAAGGA 
GCTTCAACGG GAGCGGAAGA GCCACGCACT CCCGCTCAGC TGGAGGAGGA GGAGCGTAAG 
CGAACACTTC GTTTGGAGAA TGCACCCAAA ACACATTTCG AGCCTTATGT AGACGATTTA 
ACGGGCGAAG AGATCCTGGC GCAAGTGCAA AATGCTGCGC AACAGGCCAA ACAAAGAGTG 
AACGCCAAGA AAAATGCCCA CAAGATGGGT TTCCTGCGTC AAATGAAGGA TGGCACCACC 
ACCCTGAATC TGGTGATCAA AGAGGAAAAC GAGGGTCCTG AAAGGGTAGT AACAATTGGT 
GATCTAGTTG GCAAACTGCA AAAGGGAAGC GCACAGCTAC AGACGCGACA GGTTGACAAA 
AGGAATGCCG TGCGGACAGT GAAATCCTTA AACTACGGAG CATTTGCCAG CTTTGCGCCC 
ACCTTTGATT CGCGCTTTTC CACGCTGAGT GCCGAAGAAA CGCAATTGGT TTTGCGTACG 
TATGGAGATG CCTCCAGTGC GGAATATGCG GAGAGCATCT TACAATTCAC CAAAGACTCT 
AACTACGGGA CCACAATTGC AAATGGTTTG CTGGATATTC TTACCAACGG AGAGCACAGT 
AAATCCCTGG ATGAACTATA CAACATGCAG CTTCATTCAT ACGAGCAGCG TGAGATTGAA 
AAGTGTTTTG AACAGGAGGA GGAAACTTCC AGCCAGCAGG AAACCACCGC CCAAATTGAG 
CAAGAATATG AAAAATACAA AAACACACAT GTGGACTTCA AGGGATTGCA GTCTCTTGGT 
GAACTTGGCA TCGATGTGAG CTTCCTAGAC GGCATGGAGG CTGAAATGAA GAGCTACGAG 
CTTAATCGGC GCATGCACGA GCATCTTAGT CAAAACTTGA CGCTAATCGA GAAGCTGCGA 
GTGGCGCAGC ACGATCGTTT GTCACAGCCT TTGCCGAATC ACCTGGGTCT GGTGCAGCCC 
GCCGGTCAGG AGGAGATCCA AACCGCCCAG CAATTGACGC AGCAGATCAG CGATTTGGCT 
AAGAAGTTGC CGCCTTCCGC CATCGCCGAT CCCTATGCCT TGCGAAAAGC TATGGGCATG 
TCCTATGCTG GCTTGCCGCC ACCTCGACCC GTCTCTCCGC GCGTCCAGCT GCCTGAGCTG 
CTCCAACAGC CGGTGGCCCT GCCACAGTTG CAACCTGTGG CCATGGAGAT CGACGAGTCT 
GATGAACAGC ATGGCGGTGG AGATCTGGAG AACGAACTGC GCGAGTTCCT CGAGAGCGGA 
TCTGGCCTCC ATTCTGCCAA TCCGGCGGAT GACAACAGCA TTGTTGCTCA GCTGTTGCTG 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609148.1
CDS	127..2712
Translation	MGSSKKHKKNKSERREKYEEYSQHQDPAQLQRGLKLILKVGSNATPEYSANSPMVDGGPPTAAEAMMSPVPEELQDHQGHRERHKKSKKKKKKKDREKKHKHHKEKRHRSRDRHRDAGSDEDMMAGADDAACSGFAPSSVAPPAADPDSSQDGFSFMDDDQSQPLPENILFFAGITTDNSPSNCPVTKPIAPRKLDDILMGSSPNSSSLQSSSLGLIGSSPTKPLPDLLIPSPSTPGGANSLNALTPKALEAPKTPSSSSESGREPRSCVLKLKQQKSPLNKLLEHLLRFLEKRDPHQFFAWPVTDDMAPGYSSIISRPMDFSTMRQKIDDHEYTALTEFTDDFKLMCENAIKYNHVDTVYNKAAKRLLQVGMKHLQPENLMRSLKPLSGYMRELTARELGFELSSNDMSRENNDSADEGASTGAEEPRTPAQLEEEERKRTLRLENAPKTHFEPYVDDLTGEEILAQVQNAAQQAKQRVNAKKNAHKMGFLRQMKDGTTTLNLVIKEENEGPERVVTIGDLVGKLQKGSAQLQTRQVDKRNAVRTVKSLNYGAFASFAPTFDSRFSTLSAEETQLVLRTYGDASSAEYAESILQFTKDSNYGTTIANGLLDILTNGEHSKSLDELYNMQLHSYEQREIEKCFEQEEETSSQQETTAQIEQEYEKYKNTHVDFKGLQSLGELGIDVSFLDGMEAEMKSYELNRRMHEHLSQNLTLIEKLRVAQHDRLSQPLPNHLGLVQPAGQEEIQTAQQLTQQISDLAKKLPPSAIADPYALRKAMGMSYAGLPPPRPVSPRVQLPELLQQPVALPQLQPVAMEIDESDEQHGGGDLENELREFLESGSGLHSANPADDNSIVAQLLLN

Target ORF information:

RefSeq Version	NM_135304.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG7154), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135304.3

ORF Insert Sequence:

ATGGGCTCCT CCAAGAAGCA CAAGAAAAAC AAGTCGGAGC GACGCGAGAA ATATGAAGAA 
TACAGTCAGC ACCAGGACCC GGCTCAGCTG CAACGAGGTC TAAAGCTTAT CCTTAAGGTG 
GGCTCGAATG CCACGCCGGA GTACAGTGCC AATTCGCCAA TGGTGGACGG AGGACCGCCC 
ACAGCAGCCG AGGCCATGAT GTCGCCAGTC CCCGAGGAAC TTCAAGATCA CCAGGGTCAT 
CGCGAGCGCC ACAAAAAGTC AAAGAAAAAA AAGAAGAAGA AGGATCGCGA GAAGAAGCAC 
AAACACCACA AGGAGAAACG GCACAGGAGC AGAGATAGAC ATCGGGACGC TGGATCGGAC 
GAAGATATGA TGGCCGGCGC TGACGATGCC GCTTGCAGTG GATTCGCCCC ATCGTCTGTC 
GCTCCACCGG CCGCCGATCC CGATTCCAGC CAAGATGGAT TTAGCTTTAT GGACGATGAT 
CAATCACAAC CGCTGCCAGA GAATATTTTG TTCTTCGCCG GCATCACCAC GGATAACTCG 
CCATCAAACT GCCCCGTAAC AAAGCCAATA GCTCCACGCA AGCTGGATGA TATTCTGATG 
GGCTCTTCGC CCAACTCGTC GTCGTTGCAA TCTTCCTCCC TGGGTTTAAT AGGCAGCAGT 
CCCACCAAGC CACTGCCCGA CCTTTTAATA CCTTCACCGT CGACGCCAGG AGGAGCCAAC 
TCCCTTAATG CCCTTACACC GAAGGCTCTC GAGGCGCCAA AGACGCCCAG TAGTTCCAGT 
GAGTCGGGTA GAGAGCCACG GAGCTGTGTG CTCAAGCTAA AGCAGCAGAA ATCGCCGTTA 
AACAAACTTC TGGAGCACCT GTTGCGCTTC CTGGAGAAGC GGGATCCCCA TCAGTTCTTC 
GCTTGGCCGG TGACCGATGA CATGGCGCCC GGTTACTCTT CAATAATCAG CAGACCCATG 
GACTTCTCCA CTATGCGACA GAAAATTGAT GATCACGAGT ATACCGCTTT AACCGAGTTC 
ACTGATGACT TTAAGCTGAT GTGCGAAAAT GCCATCAAGT ACAACCACGT GGATACGGTT 
TATAACAAGG CAGCTAAACG TCTACTCCAA GTTGGAATGA AACATCTGCA ACCTGAGAAC 
CTCATGAGAA GCCTGAAACC ACTATCTGGT TATATGAGGG AGCTTACAGC CAGAGAACTT 
GGCTTTGAGT TAAGTTCGAA CGATATGTCA AGGGAAAATA ACGATTCGGC GGATGAAGGA 
GCTTCAACGG GAGCGGAAGA GCCACGCACT CCCGCTCAGC TGGAGGAGGA GGAGCGTAAG 
CGAACACTTC GTTTGGAGAA TGCACCCAAA ACACATTTCG AGCCTTATGT AGACGATTTA 
ACGGGCGAAG AGATCCTGGC GCAAGTGCAA AATGCTGCGC AACAGGCCAA ACAAAGAGTG 
AACGCCAAGA AAAATGCCCA CAAGATGGGT TTCCTGCGTC AAATGAAGGA TGGCACCACC 
ACCCTGAATC TGGTGATCAA AGAGGAAAAC GAGGGTCCTG AAAGGGTAGT AACAATTGGT 
GATCTAGTTG GCAAACTGCA AAAGGGAAGC GCACAGCTAC AGACGCGACA GGTTGACAAA 
AGGAATGCCG TGCGGACAGT GAAATCCTTA AACTACGGAG CATTTGCCAG CTTTGCGCCC 
ACCTTTGATT CGCGCTTTTC CACGCTGAGT GCCGAAGAAA CGCAATTGGT TTTGCGTACG 
TATGGAGATG CCTCCAGTGC GGAATATGCG GAGAGCATCT TACAATTCAC CAAAGACTCT 
AACTACGGGA CCACAATTGC AAATGGTTTG CTGGATATTC TTACCAACGG AGAGCACAGT 
AAATCCCTGG ATGAACTATA CAACATGCAG CTTCATTCAT ACGAGCAGCG TGAGATTGAA 
AAGTGTTTTG AACAGGAGGA GGAAACTTCC AGCCAGCAGG AAACCACCGC CCAAATTGAG 
CAAGAATATG AAAAATACAA AAACACACAT GTGGACTTCA AGGGATTGCA GTCTCTTGGT 
GAACTTGGCA TCGATGTGAG CTTCCTAGAC GGCATGGAGG CTGAAATGAA GAGCTACGAG 
CTTAATCGGC GCATGCACGA GCATCTTAGT CAAAACTTGA CGCTAATCGA GAAGCTGCGA 
GTGGCGCAGC ACGATCGTTT GTCACAGCCT TTGCCGAATC ACCTGGGTCT GGTGCAGCCC 
GCCGGTCAGG AGGAGATCCA AACCGCCCAG CAATTGACGC AGCAGATCAG CGATTTGGCT 
AAGAAGTTGC CGCCTTCCGC CATCGCCGAT CCCTATGCCT TGCGAAAAGC TATGGGCATG 
TCCTATGCTG GCTTGCCGCC ACCTCGACCC GTCTCTCCGC GCGTCCAGCT GCCTGAGCTG 
CTCCAACAGC CGGTGGCCCT GCCACAGTTG CAACCTGTGG CCATGGAGAT CGACGAGTCT 
GATGAACAGC ATGGCGGTGG AGATCTGGAG AACGAACTGC GCGAGTTCCT CGAGAGCGGA 
TCTGGCCTCC ATTCTGCCAA TCCGGCGGAT GACAACAGCA TTGTTGCTCA GCTGTTGCTG 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG7154 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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