wg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wg
Entrez Gene ID	34009
Full Name	wingless
Synonyms	Br,CG4889,DWint-1,DWnt-1,Dint-1,Dm Wg,Dm-1,Dmel\CG4889,Gla,I,Sp,WG,WNT,Wg,Wnt,Wnt-1,Wnt/Wg,Wnt1,beta-catenin,dWnt,fg,int-1,l(2)02657,l(2)SH1281,l(2)SH2 1281,l(2)rO727,l(2)wg,spd,wgl,wnt,wnt1
General protein information	Preferred Names wingless Names CG4889 gene product from transcript CG4889-RA CG4889-PA Wnt-1 bristled complementation group I flag glazed lethal (2) SH1281 spade sternopleural wg-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27F1-27F1\|2-21.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078778.5	NP_523502.1	wingless

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009734	XP_553580.3
Xenopus tropicalis (tropical clawed frog)	LOC100491444	XP_002935152.1
Homo sapiens (human)	WNT1	NP_005421.1
Bos taurus (cattle)	WNT1	NP_001107663.1
Rattus norvegicus (Norway rat)	Wnt1	NP_001099184.1
Drosophila melanogaster (fruit fly)	wg	NP_523502.1
Pan troglodytes (chimpanzee)	WNT1	XP_001159566.1
	WNT1	XP_001105191.1
Canis lupus familiaris (dog)	WNT1	XP_543686.3
Mus musculus (house mouse)	Wnt1	NP_067254.1
Danio rerio (zebrafish)	wnt1	NP_001188327.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The current study further solidified the role of STAT and Wg in regeneration, by identifying their contribution to abnormal regenerations induced by IR. STAT92E (Drosophila STAT3/5) and Nurf-38, which encodes a member of the Nucleosome Remodeling Factor complex, oppose each other in these cells to modulate the frequency of ectopic disc growth
Title: STAT, Wingless, and Nurf-38 determine the accuracy of regeneration after radiation damage in Drosophila.

Notum normally functions to coordinate synaptic structural and functional differentiation via negative regulation of Wg trans-synaptic signaling in the extracellular synaptomatrix.
Title: Notum coordinates synapse development via extracellular regulation of Wingless trans-synaptic signaling.

here we examine the interactions between Msk and the Wg pathway in regulation of the AMP pool size. We find that a myoblast-specific reduction of Msk results in the absence of Vg expression and a complete loss of the Wg pathway readout beta-catenin/Armadillo (Arm). Moreover, msk RNA interference knockdown abolishes expression of the Wg target Ladybird (Lbe) in leg disc myoblasts.
Title: Adult Muscle Formation Requires

An absolute requirement for Armadillo for activation and repression of Wingless protein target genes.Pan protein regulates Wingless-responsive enhancers.
Title: Probing the canonicity of the Wnt/Wingless signaling pathway.

hypothesize that VAMP7 is required for Wg intracellular trafficking and the accumulation of Wg in Rab4-dependent recycling endosomes might affect Wg signaling
Title: Drosophila VAMP7 regulates Wingless intracellular trafficking.

The following wg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14017	NM_078778.5 Latest version!	Drosophila melanogaster wingless (wg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wg ( NM_078778.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523502.1 Drosophila melanogaster wingless (wg), mRNA.

Price & Availability↑

CloneID	OFa14017
Clone ID Related Accession (Same CDS sequence)	NM_078778.5
Accession Version	NM_078778.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wingless
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078778.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATCA GCTATATCTT CGTCATCTGC CTGATGGCCC TGTGCAGCGG CGGCAGCAGT 
CTCAGCCAAG TCGAGGGCAA ACAGAAATCC GGAAGGGGCC GGGGCTCCAT GTGGTGGGGC 
ATTGCCAAGG TCGGCGAACC CAACAACATT ACGCCCATCA TGTACATGGA CCCAGCGATC 
CACTCTACGT TGAGAAGGAA ACAGCGACGC CTGGTCAGGG ACAATCCCGG TGTACTGGGA 
GCCCTGGTCA AGGGCGCCAA CTTGGCCATT AGCGAGTGCC AACACCAGTT CAGAAATCGC 
CGCTGGAACT GCTCGACGAG AAACTTCTCG AGGGGCAAAA ATCTATTCGG CAAAATCGTT 
GATCGAGGCT GCCGAGAGAC GAGCTTCATT TACGCAATCA CCAGCGCGGC GGTGACCCAC 
TCGATTGCCA GGGCCTGCAG TGAAGGAACG ATAGAGTCCT GCACCTGCGA CTACAGCCAC 
CAGTCGAGAT CTCCACAAGC GAACCACCAG GCGGGCAGTG TGGCCGGCGT GCGGGATTGG 
GAGTGGGGCG GCTGCTCCGA CAACATCGGA TTCGGGTTCA AGTTCTCCCG GGAATTCGTC 
GATACCGGCG AGAGGGGTCG CAATCTGCGC GAGAAGATGA ATCTGCACAA CAACGAGGCG 
GGTCGAGCGC ACGTCCAAGC GGAGATGCGA CAGGAGTGCA AATGCCATGG CATGTCCGGA 
TCGTGTACAG TGAAGACCTG CTGGATGCGA CTGGCCAACT TCCGTGTGAT TGGCGACAAT 
CTGAAGGCCC GCTTCGATGG AGCCACCCGC GTGCAAGTGA CCAACAGTCT CCGGGCCACC 
AACGCTCTGG CCCCAGTTAG TCCGAATGCA GCCGGCTCGA ATTCCGTGGG CTCCAACGGC 
CTGATTATTC CGCAGTCTGG TCTGGTCTAC GGCGAGGAGG AGGAGCGTAT GCTGAACGAC 
CATATGCCGG ACATCCTGCT AGAGAACAGC CACCCGATCA GCAAGATCCA TCACCCGAAC 
ATGCCGTCGC CCAACAGTTT GCCCCAGGCT GGTCAAAGGG GCGGACGAAA TGGACGTCGT 
CAGGGACGCA AGCATAATAG ATATCACTTC CAACTGAACC CGCACAATCC CGAGCACAAG 
CCACCCGGCT CGAAGGACCT TGTCTATCTG GAGCCTTCGC CGAGCTTCTG CGAGAAGAAC 
CTGCGGCAGG GCATCCTGGG AACCCATGGC CGCCAGTGCA ATGAGACCTC GCTGGGCGTC 
GACGGCTGTG GGCTGATGTG CTGTGGGCGT GGCTATCGGC GAGACGAGGT CGTCGTTGTG 
GAGCGGTGCG CCTGCACCTT CCACTGGTGC TGCGAGGTGA AGTGCAAGCT GTGTCGGACC 
AAAAAGGTCA TCTACACGTG TCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523502.1
CDS	434..1840
Translation	MDISYIFVICLMALCSGGSSLSQVEGKQKSGRGRGSMWWGIAKVGEPNNITPIMYMDPAIHSTLRRKQRRLVRDNPGVLGALVKGANLAISECQHQFRNRRWNCSTRNFSRGKNLFGKIVDRGCRETSFIYAITSAAVTHSIARACSEGTIESCTCDYSHQSRSPQANHQAGSVAGVRDWEWGGCSDNIGFGFKFSREFVDTGERGRNLREKMNLHNNEAGRAHVQAEMRQECKCHGMSGSCTVKTCWMRLANFRVIGDNLKARFDGATRVQVTNSLRATNALAPVSPNAAGSNSVGSNGLIIPQSGLVYGEEEERMLNDHMPDILLENSHPISKIHHPNMPSPNSLPQAGQRGGRNGRRQGRKHNRYHFQLNPHNPEHKPPGSKDLVYLEPSPSFCEKNLRQGILGTHGRQCNETSLGVDGCGLMCCGRGYRRDEVVVVERCACTFHWCCEVKCKLCRTKKVIYTCL

Target ORF information:

RefSeq Version	NM_078778.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wingless (wg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078778.5

ORF Insert Sequence:

ATGGATATCA GCTATATCTT CGTCATCTGC CTGATGGCCC TGTGCAGCGG CGGCAGCAGT 
CTCAGCCAAG TCGAGGGCAA ACAGAAATCC GGAAGGGGCC GGGGCTCCAT GTGGTGGGGC 
ATTGCCAAGG TCGGCGAACC CAACAACATT ACGCCCATCA TGTACATGGA CCCAGCGATC 
CACTCTACGT TGAGAAGGAA ACAGCGACGC CTGGTCAGGG ACAATCCCGG TGTACTGGGA 
GCCCTGGTCA AGGGCGCCAA CTTGGCCATT AGCGAGTGCC AACACCAGTT CAGAAATCGC 
CGCTGGAACT GCTCGACGAG AAACTTCTCG AGGGGCAAAA ATCTATTCGG CAAAATCGTT 
GATCGAGGCT GCCGAGAGAC GAGCTTCATT TACGCAATCA CCAGCGCGGC GGTGACCCAC 
TCGATTGCCA GGGCCTGCAG TGAAGGAACG ATAGAGTCCT GCACCTGCGA CTACAGCCAC 
CAGTCGAGAT CTCCACAAGC GAACCACCAG GCGGGCAGTG TGGCCGGCGT GCGGGATTGG 
GAGTGGGGCG GCTGCTCCGA CAACATCGGA TTCGGGTTCA AGTTCTCCCG GGAATTCGTC 
GATACCGGCG AGAGGGGTCG CAATCTGCGC GAGAAGATGA ATCTGCACAA CAACGAGGCG 
GGTCGAGCGC ACGTCCAAGC GGAGATGCGA CAGGAGTGCA AATGCCATGG CATGTCCGGA 
TCGTGTACAG TGAAGACCTG CTGGATGCGA CTGGCCAACT TCCGTGTGAT TGGCGACAAT 
CTGAAGGCCC GCTTCGATGG AGCCACCCGC GTGCAAGTGA CCAACAGTCT CCGGGCCACC 
AACGCTCTGG CCCCAGTTAG TCCGAATGCA GCCGGCTCGA ATTCCGTGGG CTCCAACGGC 
CTGATTATTC CGCAGTCTGG TCTGGTCTAC GGCGAGGAGG AGGAGCGTAT GCTGAACGAC 
CATATGCCGG ACATCCTGCT AGAGAACAGC CACCCGATCA GCAAGATCCA TCACCCGAAC 
ATGCCGTCGC CCAACAGTTT GCCCCAGGCT GGTCAAAGGG GCGGACGAAA TGGACGTCGT 
CAGGGACGCA AGCATAATAG ATATCACTTC CAACTGAACC CGCACAATCC CGAGCACAAG 
CCACCCGGCT CGAAGGACCT TGTCTATCTG GAGCCTTCGC CGAGCTTCTG CGAGAAGAAC 
CTGCGGCAGG GCATCCTGGG AACCCATGGC CGCCAGTGCA ATGAGACCTC GCTGGGCGTC 
GACGGCTGTG GGCTGATGTG CTGTGGGCGT GGCTATCGGC GAGACGAGGT CGTCGTTGTG 
GAGCGGTGCG CCTGCACCTT CCACTGGTGC TGCGAGGTGA AGTGCAAGCT GTGTCGGACC 
AAAAAGGTCA TCTACACGTG TCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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