Tsp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tsp
Entrez Gene ID	33941
Full Name	Thrombospondin
Synonyms	CG11326,CT31613,D-TSP,DTSP,Dmel\CG11326,SP295,tsp
General protein information	Preferred Names Thrombospondin Names CG11326 gene product from transcript CG11326-RF CG11326-PA CG11326-PC CG11326-PD CG11326-PE CG11326-PF CG11326-PG CG11326-PH Tsp-PA Tsp-PC Tsp-PD Tsp-PE Tsp-PF Tsp-PG Tsp-PH thrombospondin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26F7-27A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_078771.3	NP_523495.2	thrombospondin, isoform A
NM_164703.2	NP_723204.1	thrombospondin, isoform D
NM_001273219.1	NP_001260148.1	thrombospondin, isoform H
NM_164704.2	NP_723205.1	thrombospondin, isoform E
NM_164702.2	NP_723203.1	thrombospondin, isoform C
NM_001144330.2	NP_001137802.1	thrombospondin, isoform F
NM_001144331.2	NP_001137803.1	thrombospondin, isoform G

Pathways from BioSystems

Homologies

	COMP	XP_001115565.1
Canis lupus familiaris (dog)	COMP	XP_533869.2
Gallus gallus (chicken)	COMP	XP_418238.2
Homo sapiens (human)	COMP	NP_000086.2
Danio rerio (zebrafish)	im:7154332	XP_002663752.3
Rattus norvegicus (Norway rat)	Comp	NP_036966.1
Drosophila melanogaster (fruit fly)	Tsp	NP_523495.2
Xenopus tropicalis (tropical clawed frog)	comp	XP_002940251.2
Pan troglodytes (chimpanzee)	COMP	NP_001092035.1
Bos taurus (cattle)	COMP	NP_001159989.1
Mus musculus (house mouse)	Comp	NP_057894.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002157	XP_308033.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thrombospondin-mediated adhesion is essential for the formation of the myotendinous junction in Drosophila.
Title: Thrombospondin-mediated adhesion is essential for the formation of the myotendinous junction in Drosophila.

The following Tsp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tsp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13951	NM_001273219.1 Latest version!	Drosophila melanogaster thrombospondin, transcript variant H (Tsp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13951	NM_001144331.2 Latest version!	Drosophila melanogaster thrombospondin, transcript variant G (Tsp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16319	NM_164703.2 Latest version!	Drosophila melanogaster thrombospondin, transcript variant D (Tsp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16319	NM_164704.2 Latest version!	Drosophila melanogaster thrombospondin, transcript variant E (Tsp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16319	NM_164702.2 Latest version!	Drosophila melanogaster thrombospondin, transcript variant C (Tsp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13950	NM_078771.3 Latest version!	Drosophila melanogaster thrombospondin, transcript variant A (Tsp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa13950	NM_001144330.2 Latest version!	Drosophila melanogaster thrombospondin, transcript variant F (Tsp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tsp ( NM_001273219.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260148.1 Drosophila melanogaster thrombospondin, transcript variant H (Tsp), mRNA.

Price & Availability↑

CloneID	OFa13951
Clone ID Related Accession (Same CDS sequence)	NM_001273219.1 , NM_001144331.2
Accession Version	NM_001273219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thrombospondin, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCCACG AGGTGAACGG AACCAGCTGC CGGGATATGA ATGAGTGCCT GCTCTACGAT 
CCTTGCGACG AGCTGGCGAC GTGCACCAAT CTTAGTCCTG GCTTCCAGTG CAGCCCGTGT 
CCCGTTGGCT TCGATGGCAC CCACGCCCAT GGCTACTTCG CCGACTACTA CTCCGTGGAT 
TATCGCAGGC AAACGTGCCT GGACGTCGAT GAGTGCCGCA CTGGGTTCTT CCGATGCCCG 
GAGCATTCTA CTTGCATCAA CGAGATTGGT TCCTACAGAT GCCAATGCCA CGAAGGATAT 
GTAACAAATG GCACCTACAG CTGCCTAGAC AGATCCTCGG TGTTCATGTG TCCGGATGGA 
ACCGTTTGTG ACCGCAATGC CGTGTGCCTT AGGATGGATA ACATCCGCCA CAAGTGTCAC 
TGCAATGTGG GATGGGCGGG CAACGGTTTG ATTTGCGGTA GGGATACCGA CGTAGATGGA 
TGGCCAGATC AGGCAATCGG TTGTCCAGAG CTGCGCTGTC AGCGAGACAA CTGCCCGAAG 
TTGCCTAATT CAGGCCAGGA GGACGCCGAT CTGGATGGAC ATGGCGATGG GTGCGACGAC 
GATGCCGATG GCGACAATGT CCAGAACAGC CAAGACAACT GCTGGCTGGC CTACAACACC 
GAGCAGCTGG ACTCGGATGG AGACAAGGTG GGCGATGTGT GCGACAACTG CGTGCTTAAG 
TACAATCCCA GGCAGTTGGA CACGGACGAG GATGGACTGG GAGACGAGTG CGATGGTGAT 
ATCGACAACG ACTCCATACC AAATGCGCTA GACAACTGTC CGCTGCTGCC GAATCCCAGT 
CAGTCGGACG TGGACAACGA TGGTGTGGGC GACGCTTGCG ATAACTGTCC GAATCTTCCG 
AATCCCGACC AAAAGGATCG CGACATGGAC TTTGTAGGCG ATGCCTGTCA CAGAGACATC 
GACGGGGATG ATGATGGAGT TCCAAACTCG CTGGACAACT GCCCAATGGT CAGTAACTCG 
GACCAGCTGG ACACTGACGG TGATGGAACG GGCGATGAGT GCGATGACGA CATGGACGGA 
GATGGGATAC CCAACTACAA GGACAACTGT CCGCTGGCCA AGAACCCAAA GCAGGACGAC 
TTCAATCGGA ATGGCAAGGG TGACTCTTGC GAAGATGATG AGGACGTAGA TGGTGTACCG 
AATGGCATGG ACAACTGCCC GAACAACTCG ATGATTCATC ACACTGACTT TCGCACTCTT 
CAAACCATTC CACTGGATCC CAAAGGCCTA TCGCAAGCGG ATCCCAACTG GGTGGTACAC 
GCCAATGGCA CCGAGATAGT CCAGACCCTC AATTCGGATC CGGGACTAGC GGTGGGCAAG 
GACGCCTTCG GAGGCGTTGA CTTCGACGGC ACCTTTTATA TCAACGATGA CACGGACGAT 
GACTACGCTG GCTTTGTGTT CAGCTACCAG AGCAGCTACA AGTACTACGT GGTGCAGTGG 
AAGAAGGGCA CCCAAACCTA CTGGGAGCCA CGACCCTTCA CCGCCTCTGC GGCGCCGGGC 
ATCCAGATCA AGCTGGTCAA CAGCACCGAG GGTCCGGGCC CAATGATGAG GAACAGCCTG 
TGGCACGAGG GAAACACCGA CGGTGAGGCC AGGCTGCTGT GGAAGGATCC GAAGAACATT 
GCGTGGAAGG AAAGGACCTC CTACCGCTGG TCCCTGGTGC ATCGACCGGC CATCGGCCTC 
ATCCGCCTGC AAATGCACGA GGGTAACCGC CTGATCTTCG ACTCGGGCAA TGTGTTTGAC 
TCCACGCTGA AGGGCGGTCG GCTGGGCGTG TTCTGCTTCT CACAGAGGAT GATTATATGG 
TCCAACCTGC AGTACAAGTG CAATAATCGT GTGAAGCCGC TGATCTACAA TGATCTGTCC 
GACTATCTCA AGACGAAGGT GGAGTTGCAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260148.1
CDS	275..2230
Translation	MGHEVNGTSCRDMNECLLYDPCDELATCTNLSPGFQCSPCPVGFDGTHAHGYFADYYSVDYRRQTCLDVDECRTGFFRCPEHSTCINEIGSYRCQCHEGYVTNGTYSCLDRSSVFMCPDGTVCDRNAVCLRMDNIRHKCHCNVGWAGNGLICGRDTDVDGWPDQAIGCPELRCQRDNCPKLPNSGQEDADLDGHGDGCDDDADGDNVQNSQDNCWLAYNTEQLDSDGDKVGDVCDNCVLKYNPRQLDTDEDGLGDECDGDIDNDSIPNALDNCPLLPNPSQSDVDNDGVGDACDNCPNLPNPDQKDRDMDFVGDACHRDIDGDDDGVPNSLDNCPMVSNSDQLDTDGDGTGDECDDDMDGDGIPNYKDNCPLAKNPKQDDFNRNGKGDSCEDDEDVDGVPNGMDNCPNNSMIHHTDFRTLQTIPLDPKGLSQADPNWVVHANGTEIVQTLNSDPGLAVGKDAFGGVDFDGTFYINDDTDDDYAGFVFSYQSSYKYYVVQWKKGTQTYWEPRPFTASAAPGIQIKLVNSTEGPGPMMRNSLWHEGNTDGEARLLWKDPKNIAWKERTSYRWSLVHRPAIGLIRLQMHEGNRLIFDSGNVFDSTLKGGRLGVFCFSQRMIIWSNLQYKCNNRVKPLIYNDLSDYLKTKVELQD

Target ORF information:

RefSeq Version	NM_001273219.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thrombospondin, transcript variant H (Tsp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273219.1

ORF Insert Sequence:

ATGGGCCACG AGGTGAACGG AACCAGCTGC CGGGATATGA ATGAGTGCCT GCTCTACGAT 
CCTTGCGACG AGCTGGCGAC GTGCACCAAT CTTAGTCCTG GCTTCCAGTG CAGCCCGTGT 
CCCGTTGGCT TCGATGGCAC CCACGCCCAT GGCTACTTCG CCGACTACTA CTCCGTGGAT 
TATCGCAGGC AAACGTGCCT GGACGTCGAT GAGTGCCGCA CTGGGTTCTT CCGATGCCCG 
GAGCATTCTA CTTGCATCAA CGAGATTGGT TCCTACAGAT GCCAATGCCA CGAAGGATAT 
GTAACAAATG GCACCTACAG CTGCCTAGAC AGATCCTCGG TGTTCATGTG TCCGGATGGA 
ACCGTTTGTG ACCGCAATGC CGTGTGCCTT AGGATGGATA ACATCCGCCA CAAGTGTCAC 
TGCAATGTGG GATGGGCGGG CAACGGTTTG ATTTGCGGTA GGGATACCGA CGTAGATGGA 
TGGCCAGATC AGGCAATCGG TTGTCCAGAG CTGCGCTGTC AGCGAGACAA CTGCCCGAAG 
TTGCCTAATT CAGGCCAGGA GGACGCCGAT CTGGATGGAC ATGGCGATGG GTGCGACGAC 
GATGCCGATG GCGACAATGT CCAGAACAGC CAAGACAACT GCTGGCTGGC CTACAACACC 
GAGCAGCTGG ACTCGGATGG AGACAAGGTG GGCGATGTGT GCGACAACTG CGTGCTTAAG 
TACAATCCCA GGCAGTTGGA CACGGACGAG GATGGACTGG GAGACGAGTG CGATGGTGAT 
ATCGACAACG ACTCCATACC AAATGCGCTA GACAACTGTC CGCTGCTGCC GAATCCCAGT 
CAGTCGGACG TGGACAACGA TGGTGTGGGC GACGCTTGCG ATAACTGTCC GAATCTTCCG 
AATCCCGACC AAAAGGATCG CGACATGGAC TTTGTAGGCG ATGCCTGTCA CAGAGACATC 
GACGGGGATG ATGATGGAGT TCCAAACTCG CTGGACAACT GCCCAATGGT CAGTAACTCG 
GACCAGCTGG ACACTGACGG TGATGGAACG GGCGATGAGT GCGATGACGA CATGGACGGA 
GATGGGATAC CCAACTACAA GGACAACTGT CCGCTGGCCA AGAACCCAAA GCAGGACGAC 
TTCAATCGGA ATGGCAAGGG TGACTCTTGC GAAGATGATG AGGACGTAGA TGGTGTACCG 
AATGGCATGG ACAACTGCCC GAACAACTCG ATGATTCATC ACACTGACTT TCGCACTCTT 
CAAACCATTC CACTGGATCC CAAAGGCCTA TCGCAAGCGG ATCCCAACTG GGTGGTACAC 
GCCAATGGCA CCGAGATAGT CCAGACCCTC AATTCGGATC CGGGACTAGC GGTGGGCAAG 
GACGCCTTCG GAGGCGTTGA CTTCGACGGC ACCTTTTATA TCAACGATGA CACGGACGAT 
GACTACGCTG GCTTTGTGTT CAGCTACCAG AGCAGCTACA AGTACTACGT GGTGCAGTGG 
AAGAAGGGCA CCCAAACCTA CTGGGAGCCA CGACCCTTCA CCGCCTCTGC GGCGCCGGGC 
ATCCAGATCA AGCTGGTCAA CAGCACCGAG GGTCCGGGCC CAATGATGAG GAACAGCCTG 
TGGCACGAGG GAAACACCGA CGGTGAGGCC AGGCTGCTGT GGAAGGATCC GAAGAACATT 
GCGTGGAAGG AAAGGACCTC CTACCGCTGG TCCCTGGTGC ATCGACCGGC CATCGGCCTC 
ATCCGCCTGC AAATGCACGA GGGTAACCGC CTGATCTTCG ACTCGGGCAA TGTGTTTGAC 
TCCACGCTGA AGGGCGGTCG GCTGGGCGTG TTCTGCTTCT CACAGAGGAT GATTATATGG 
TCCAACCTGC AGTACAAGTG CAATAATCGT GTGAAGCCGC TGATCTACAA TGATCTGTCC 
GACTATCTCA AGACGAAGGT GGAGTTGCAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsp ( NM_001144331.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137803.1 Drosophila melanogaster thrombospondin, transcript variant G (Tsp), mRNA.

Price & Availability↑

CloneID	OFa13951
Clone ID Related Accession (Same CDS sequence)	NM_001273219.1 , NM_001144331.2
Accession Version	NM_001144331.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thrombospondin, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001144331.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCCACG AGGTGAACGG AACCAGCTGC CGGGATATGA ATGAGTGCCT GCTCTACGAT 
CCTTGCGACG AGCTGGCGAC GTGCACCAAT CTTAGTCCTG GCTTCCAGTG CAGCCCGTGT 
CCCGTTGGCT TCGATGGCAC CCACGCCCAT GGCTACTTCG CCGACTACTA CTCCGTGGAT 
TATCGCAGGC AAACGTGCCT GGACGTCGAT GAGTGCCGCA CTGGGTTCTT CCGATGCCCG 
GAGCATTCTA CTTGCATCAA CGAGATTGGT TCCTACAGAT GCCAATGCCA CGAAGGATAT 
GTAACAAATG GCACCTACAG CTGCCTAGAC AGATCCTCGG TGTTCATGTG TCCGGATGGA 
ACCGTTTGTG ACCGCAATGC CGTGTGCCTT AGGATGGATA ACATCCGCCA CAAGTGTCAC 
TGCAATGTGG GATGGGCGGG CAACGGTTTG ATTTGCGGTA GGGATACCGA CGTAGATGGA 
TGGCCAGATC AGGCAATCGG TTGTCCAGAG CTGCGCTGTC AGCGAGACAA CTGCCCGAAG 
TTGCCTAATT CAGGCCAGGA GGACGCCGAT CTGGATGGAC ATGGCGATGG GTGCGACGAC 
GATGCCGATG GCGACAATGT CCAGAACAGC CAAGACAACT GCTGGCTGGC CTACAACACC 
GAGCAGCTGG ACTCGGATGG AGACAAGGTG GGCGATGTGT GCGACAACTG CGTGCTTAAG 
TACAATCCCA GGCAGTTGGA CACGGACGAG GATGGACTGG GAGACGAGTG CGATGGTGAT 
ATCGACAACG ACTCCATACC AAATGCGCTA GACAACTGTC CGCTGCTGCC GAATCCCAGT 
CAGTCGGACG TGGACAACGA TGGTGTGGGC GACGCTTGCG ATAACTGTCC GAATCTTCCG 
AATCCCGACC AAAAGGATCG CGACATGGAC TTTGTAGGCG ATGCCTGTCA CAGAGACATC 
GACGGGGATG ATGATGGAGT TCCAAACTCG CTGGACAACT GCCCAATGGT CAGTAACTCG 
GACCAGCTGG ACACTGACGG TGATGGAACG GGCGATGAGT GCGATGACGA CATGGACGGA 
GATGGGATAC CCAACTACAA GGACAACTGT CCGCTGGCCA AGAACCCAAA GCAGGACGAC 
TTCAATCGGA ATGGCAAGGG TGACTCTTGC GAAGATGATG AGGACGTAGA TGGTGTACCG 
AATGGCATGG ACAACTGCCC GAACAACTCG ATGATTCATC ACACTGACTT TCGCACTCTT 
CAAACCATTC CACTGGATCC CAAAGGCCTA TCGCAAGCGG ATCCCAACTG GGTGGTACAC 
GCCAATGGCA CCGAGATAGT CCAGACCCTC AATTCGGATC CGGGACTAGC GGTGGGCAAG 
GACGCCTTCG GAGGCGTTGA CTTCGACGGC ACCTTTTATA TCAACGATGA CACGGACGAT 
GACTACGCTG GCTTTGTGTT CAGCTACCAG AGCAGCTACA AGTACTACGT GGTGCAGTGG 
AAGAAGGGCA CCCAAACCTA CTGGGAGCCA CGACCCTTCA CCGCCTCTGC GGCGCCGGGC 
ATCCAGATCA AGCTGGTCAA CAGCACCGAG GGTCCGGGCC CAATGATGAG GAACAGCCTG 
TGGCACGAGG GAAACACCGA CGGTGAGGCC AGGCTGCTGT GGAAGGATCC GAAGAACATT 
GCGTGGAAGG AAAGGACCTC CTACCGCTGG TCCCTGGTGC ATCGACCGGC CATCGGCCTC 
ATCCGCCTGC AAATGCACGA GGGTAACCGC CTGATCTTCG ACTCGGGCAA TGTGTTTGAC 
TCCACGCTGA AGGGCGGTCG GCTGGGCGTG TTCTGCTTCT CACAGAGGAT GATTATATGG 
TCCAACCTGC AGTACAAGTG CAATAATCGT GTGAAGCCGC TGATCTACAA TGATCTGTCC 
GACTATCTCA AGACGAAGGT GGAGTTGCAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137803.1
CDS	223..2178
Translation	MGHEVNGTSCRDMNECLLYDPCDELATCTNLSPGFQCSPCPVGFDGTHAHGYFADYYSVDYRRQTCLDVDECRTGFFRCPEHSTCINEIGSYRCQCHEGYVTNGTYSCLDRSSVFMCPDGTVCDRNAVCLRMDNIRHKCHCNVGWAGNGLICGRDTDVDGWPDQAIGCPELRCQRDNCPKLPNSGQEDADLDGHGDGCDDDADGDNVQNSQDNCWLAYNTEQLDSDGDKVGDVCDNCVLKYNPRQLDTDEDGLGDECDGDIDNDSIPNALDNCPLLPNPSQSDVDNDGVGDACDNCPNLPNPDQKDRDMDFVGDACHRDIDGDDDGVPNSLDNCPMVSNSDQLDTDGDGTGDECDDDMDGDGIPNYKDNCPLAKNPKQDDFNRNGKGDSCEDDEDVDGVPNGMDNCPNNSMIHHTDFRTLQTIPLDPKGLSQADPNWVVHANGTEIVQTLNSDPGLAVGKDAFGGVDFDGTFYINDDTDDDYAGFVFSYQSSYKYYVVQWKKGTQTYWEPRPFTASAAPGIQIKLVNSTEGPGPMMRNSLWHEGNTDGEARLLWKDPKNIAWKERTSYRWSLVHRPAIGLIRLQMHEGNRLIFDSGNVFDSTLKGGRLGVFCFSQRMIIWSNLQYKCNNRVKPLIYNDLSDYLKTKVELQD

Target ORF information:

RefSeq Version	NM_001144331.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thrombospondin, transcript variant G (Tsp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144331.2

ORF Insert Sequence:

ATGGGCCACG AGGTGAACGG AACCAGCTGC CGGGATATGA ATGAGTGCCT GCTCTACGAT 
CCTTGCGACG AGCTGGCGAC GTGCACCAAT CTTAGTCCTG GCTTCCAGTG CAGCCCGTGT 
CCCGTTGGCT TCGATGGCAC CCACGCCCAT GGCTACTTCG CCGACTACTA CTCCGTGGAT 
TATCGCAGGC AAACGTGCCT GGACGTCGAT GAGTGCCGCA CTGGGTTCTT CCGATGCCCG 
GAGCATTCTA CTTGCATCAA CGAGATTGGT TCCTACAGAT GCCAATGCCA CGAAGGATAT 
GTAACAAATG GCACCTACAG CTGCCTAGAC AGATCCTCGG TGTTCATGTG TCCGGATGGA 
ACCGTTTGTG ACCGCAATGC CGTGTGCCTT AGGATGGATA ACATCCGCCA CAAGTGTCAC 
TGCAATGTGG GATGGGCGGG CAACGGTTTG ATTTGCGGTA GGGATACCGA CGTAGATGGA 
TGGCCAGATC AGGCAATCGG TTGTCCAGAG CTGCGCTGTC AGCGAGACAA CTGCCCGAAG 
TTGCCTAATT CAGGCCAGGA GGACGCCGAT CTGGATGGAC ATGGCGATGG GTGCGACGAC 
GATGCCGATG GCGACAATGT CCAGAACAGC CAAGACAACT GCTGGCTGGC CTACAACACC 
GAGCAGCTGG ACTCGGATGG AGACAAGGTG GGCGATGTGT GCGACAACTG CGTGCTTAAG 
TACAATCCCA GGCAGTTGGA CACGGACGAG GATGGACTGG GAGACGAGTG CGATGGTGAT 
ATCGACAACG ACTCCATACC AAATGCGCTA GACAACTGTC CGCTGCTGCC GAATCCCAGT 
CAGTCGGACG TGGACAACGA TGGTGTGGGC GACGCTTGCG ATAACTGTCC GAATCTTCCG 
AATCCCGACC AAAAGGATCG CGACATGGAC TTTGTAGGCG ATGCCTGTCA CAGAGACATC 
GACGGGGATG ATGATGGAGT TCCAAACTCG CTGGACAACT GCCCAATGGT CAGTAACTCG 
GACCAGCTGG ACACTGACGG TGATGGAACG GGCGATGAGT GCGATGACGA CATGGACGGA 
GATGGGATAC CCAACTACAA GGACAACTGT CCGCTGGCCA AGAACCCAAA GCAGGACGAC 
TTCAATCGGA ATGGCAAGGG TGACTCTTGC GAAGATGATG AGGACGTAGA TGGTGTACCG 
AATGGCATGG ACAACTGCCC GAACAACTCG ATGATTCATC ACACTGACTT TCGCACTCTT 
CAAACCATTC CACTGGATCC CAAAGGCCTA TCGCAAGCGG ATCCCAACTG GGTGGTACAC 
GCCAATGGCA CCGAGATAGT CCAGACCCTC AATTCGGATC CGGGACTAGC GGTGGGCAAG 
GACGCCTTCG GAGGCGTTGA CTTCGACGGC ACCTTTTATA TCAACGATGA CACGGACGAT 
GACTACGCTG GCTTTGTGTT CAGCTACCAG AGCAGCTACA AGTACTACGT GGTGCAGTGG 
AAGAAGGGCA CCCAAACCTA CTGGGAGCCA CGACCCTTCA CCGCCTCTGC GGCGCCGGGC 
ATCCAGATCA AGCTGGTCAA CAGCACCGAG GGTCCGGGCC CAATGATGAG GAACAGCCTG 
TGGCACGAGG GAAACACCGA CGGTGAGGCC AGGCTGCTGT GGAAGGATCC GAAGAACATT 
GCGTGGAAGG AAAGGACCTC CTACCGCTGG TCCCTGGTGC ATCGACCGGC CATCGGCCTC 
ATCCGCCTGC AAATGCACGA GGGTAACCGC CTGATCTTCG ACTCGGGCAA TGTGTTTGAC 
TCCACGCTGA AGGGCGGTCG GCTGGGCGTG TTCTGCTTCT CACAGAGGAT GATTATATGG 
TCCAACCTGC AGTACAAGTG CAATAATCGT GTGAAGCCGC TGATCTACAA TGATCTGTCC 
GACTATCTCA AGACGAAGGT GGAGTTGCAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsp ( NM_164703.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723204.1 Drosophila melanogaster thrombospondin, transcript variant D (Tsp), mRNA.

Price & Availability↑

CloneID	OFa16319
Clone ID Related Accession (Same CDS sequence)	NM_164703.2 , NM_164704.2 , NM_164702.2
Accession Version	NM_164703.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thrombospondin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164703.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCAACATTC TAGTGCCCTC GCATAATGTG 
CAACACTATC AGAGGCAGAG CCAGTACACC GCCCGAAAAT ACTCGGTCGT TACGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723204.1
CDS	437..1696
Translation	MNWTRVLLIGLTALALTFVDVASLSLDPVASAELEQFIRKGDVVISTRHIRPRRKLHISIEALFMIDFPMLKHKMSFFLDRKQQRVTLDISANGATESRNFEIPNINETSTIRSLALQFSKNRITLHVDCKASTHHDIDMNLAKLYTQMDDPVIKLFRERKYPLHFDGDMEHSLQRANCQKGNHRRGNRRMLRNKITEREKNKKRDVRGWYEPTIAREGVVDHRHQEVPTDVERGDIPVLNGDCEDALARSLSDLLALVKLLREDVAHQRQEIAYLRMLLENCAGCKNPLTTDNQLRIEPDCRSANPCYPGVECLDSAAGPRCGHCPLGFIGDGKSCKPGVTCAHHMCYPGVQCHDTVNGAQCDSCPAGYEGDGRTCSLRNPCLDTPCPSGNILVPSHNVQHYQRQSQYTARKYSVVTN

Target ORF information:

RefSeq Version	NM_164703.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thrombospondin, transcript variant D (Tsp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164703.2

ORF Insert Sequence:

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCAACATTC TAGTGCCCTC GCATAATGTG 
CAACACTATC AGAGGCAGAG CCAGTACACC GCCCGAAAAT ACTCGGTCGT TACGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsp ( NM_164704.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723205.1 Drosophila melanogaster thrombospondin, transcript variant E (Tsp), mRNA.

Price & Availability↑

CloneID	OFa16319
Clone ID Related Accession (Same CDS sequence)	NM_164703.2 , NM_164704.2 , NM_164702.2
Accession Version	NM_164704.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thrombospondin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164704.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCAACATTC TAGTGCCCTC GCATAATGTG 
CAACACTATC AGAGGCAGAG CCAGTACACC GCCCGAAAAT ACTCGGTCGT TACGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723205.1
CDS	427..1686
Translation	MNWTRVLLIGLTALALTFVDVASLSLDPVASAELEQFIRKGDVVISTRHIRPRRKLHISIEALFMIDFPMLKHKMSFFLDRKQQRVTLDISANGATESRNFEIPNINETSTIRSLALQFSKNRITLHVDCKASTHHDIDMNLAKLYTQMDDPVIKLFRERKYPLHFDGDMEHSLQRANCQKGNHRRGNRRMLRNKITEREKNKKRDVRGWYEPTIAREGVVDHRHQEVPTDVERGDIPVLNGDCEDALARSLSDLLALVKLLREDVAHQRQEIAYLRMLLENCAGCKNPLTTDNQLRIEPDCRSANPCYPGVECLDSAAGPRCGHCPLGFIGDGKSCKPGVTCAHHMCYPGVQCHDTVNGAQCDSCPAGYEGDGRTCSLRNPCLDTPCPSGNILVPSHNVQHYQRQSQYTARKYSVVTN

Target ORF information:

RefSeq Version	NM_164704.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thrombospondin, transcript variant E (Tsp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164704.2

ORF Insert Sequence:

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCAACATTC TAGTGCCCTC GCATAATGTG 
CAACACTATC AGAGGCAGAG CCAGTACACC GCCCGAAAAT ACTCGGTCGT TACGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsp ( NM_164702.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723203.1 Drosophila melanogaster thrombospondin, transcript variant C (Tsp), mRNA.

Price & Availability↑

CloneID	OFa16319
Clone ID Related Accession (Same CDS sequence)	NM_164703.2 , NM_164704.2 , NM_164702.2
Accession Version	NM_164702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thrombospondin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164702.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCAACATTC TAGTGCCCTC GCATAATGTG 
CAACACTATC AGAGGCAGAG CCAGTACACC GCCCGAAAAT ACTCGGTCGT TACGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723203.1
CDS	450..1709
Translation	MNWTRVLLIGLTALALTFVDVASLSLDPVASAELEQFIRKGDVVISTRHIRPRRKLHISIEALFMIDFPMLKHKMSFFLDRKQQRVTLDISANGATESRNFEIPNINETSTIRSLALQFSKNRITLHVDCKASTHHDIDMNLAKLYTQMDDPVIKLFRERKYPLHFDGDMEHSLQRANCQKGNHRRGNRRMLRNKITEREKNKKRDVRGWYEPTIAREGVVDHRHQEVPTDVERGDIPVLNGDCEDALARSLSDLLALVKLLREDVAHQRQEIAYLRMLLENCAGCKNPLTTDNQLRIEPDCRSANPCYPGVECLDSAAGPRCGHCPLGFIGDGKSCKPGVTCAHHMCYPGVQCHDTVNGAQCDSCPAGYEGDGRTCSLRNPCLDTPCPSGNILVPSHNVQHYQRQSQYTARKYSVVTN

Target ORF information:

RefSeq Version	NM_164702.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thrombospondin, transcript variant C (Tsp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164702.2

ORF Insert Sequence:

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCAACATTC TAGTGCCCTC GCATAATGTG 
CAACACTATC AGAGGCAGAG CCAGTACACC GCCCGAAAAT ACTCGGTCGT TACGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsp ( NM_078771.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523495.2 Drosophila melanogaster thrombospondin, transcript variant A (Tsp), mRNA.

Price & Availability↑

CloneID	OFa13950
Clone ID Related Accession (Same CDS sequence)	NM_078771.3 , NM_001144330.2
Accession Version	NM_078771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thrombospondin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078771.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCGCCCAGT GCCTCCAGGT TGGCTATCCG 
CCCTACTTTC ACTGCATCTC GTGTCCAATG GGCCACGAGG TGAACGGAAC CAGCTGCCGG 
GATATGAATG AGTGCCTGCT CTACGATCCT TGCGACGAGC TGGCGACGTG CACCAATCTT 
AGTCCTGGCT TCCAGTGCAG CCCGTGTCCC GTTGGCTTCG ATGGCACCCA CGCCCATGGC 
TACTTCGCCG ACTACTACTC CGTGGATTAT CGCAGGCAAA CGTGCCTGGA CGTCGATGAG 
TGCCGCACTG GGTTCTTCCG ATGCCCGGAG CATTCTACTT GCATCAACGA GATTGGTTCC 
TACAGATGCC AATGCCACGA AGGATATGTA ACAAATGGCA CCTACAGCTG CCTAGACAGA 
TCCTCGGTGT TCATGTGTCC GGATGGAACC GTTTGTGACC GCAATGCCGT GTGCCTTAGG 
ATGGATAACA TCCGCCACAA GTGTCACTGC AATGTGGGAT GGGCGGGCAA CGGTTTGATT 
TGCGGTAGGG ATACCGACGT AGATGGATGG CCAGATCAGG CAATCGGTTG TCCAGAGCTG 
CGCTGTCAGC GAGACAACTG CCCGAAGTTG CCTAATTCAG GCCAGGAGGA CGCCGATCTG 
GATGGACATG GCGATGGGTG CGACGACGAT GCCGATGGCG ACAATGTCCA GAACAGCCAA 
GACAACTGCT GGCTGGCCTA CAACACCGAG CAGCTGGACT CGGATGGAGA CAAGGTGGGC 
GATGTGTGCG ACAACTGCGT GCTTAAGTAC AATCCCAGGC AGTTGGACAC GGACGAGGAT 
GGACTGGGAG ACGAGTGCGA TGGTGATATC GACAACGACT CCATACCAAA TGCGCTAGAC 
AACTGTCCGC TGCTGCCGAA TCCCAGTCAG TCGGACGTGG ACAACGATGG TGTGGGCGAC 
GCTTGCGATA ACTGTCCGAA TCTTCCGAAT CCCGACCAAA AGGATCGCGA CATGGACTTT 
GTAGGCGATG CCTGTCACAG AGACATCGAC GGGGATGATG ATGGAGTTCC AAACTCGCTG 
GACAACTGCC CAATGGTCAG TAACTCGGAC CAGCTGGACA CTGACGGTGA TGGAACGGGC 
GATGAGTGCG ATGACGACAT GGACGGAGAT GGGATACCCA ACTACAAGGA CAACTGTCCG 
CTGGCCAAGA ACCCAAAGCA GGACGACTTC AATCGGAATG GCAAGGGTGA CTCTTGCGAA 
GATGATGAGG ACGTAGATGG TGTACCGAAT GGCATGGACA ACTGCCCGAA CAACTCGATG 
ATTCATCACA CTGACTTTCG CACTCTTCAA ACCATTCCAC TGGATCCCAA AGGCCTATCG 
CAAGCGGATC CCAACTGGGT GGTACACGCC AATGGCACCG AGATAGTCCA GACCCTCAAT 
TCGGATCCGG GACTAGCGGT GGGCAAGGAC GCCTTCGGAG GCGTTGACTT CGACGGCACC 
TTTTATATCA ACGATGACAC GGACGATGAC TACGCTGGCT TTGTGTTCAG CTACCAGAGC 
AGCTACAAGT ACTACGTGGT GCAGTGGAAG AAGGGCACCC AAACCTACTG GGAGCCACGA 
CCCTTCACCG CCTCTGCGGC GCCGGGCATC CAGATCAAGC TGGTCAACAG CACCGAGGGT 
CCGGGCCCAA TGATGAGGAA CAGCCTGTGG CACGAGGGAA ACACCGACGG TGAGGCCAGG 
CTGCTGTGGA AGGATCCGAA GAACATTGCG TGGAAGGAAA GGACCTCCTA CCGCTGGTCC 
CTGGTGCATC GACCGGCCAT CGGCCTCATC CGCCTGCAAA TGCACGAGGG TAACCGCCTG 
ATCTTCGACT CGGGCAATGT GTTTGACTCC ACGCTGAAGG GCGGTCGGCT GGGCGTGTTC 
TGCTTCTCAC AGAGGATGAT TATATGGTCC AACCTGCAGT ACAAGTGCAA TAATCGTGTG 
AAGCCGCTGA TCTACAATGA TCTGTCCGAC TATCTCAAGA CGAAGGTGGA GTTGCAGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523495.2
CDS	911..4093
Translation	MNWTRVLLIGLTALALTFVDVASLSLDPVASAELEQFIRKGDVVISTRHIRPRRKLHISIEALFMIDFPMLKHKMSFFLDRKQQRVTLDISANGATESRNFEIPNINETSTIRSLALQFSKNRITLHVDCKASTHHDIDMNLAKLYTQMDDPVIKLFRERKYPLHFDGDMEHSLQRANCQKGNHRRGNRRMLRNKITEREKNKKRDVRGWYEPTIAREGVVDHRHQEVPTDVERGDIPVLNGDCEDALARSLSDLLALVKLLREDVAHQRQEIAYLRMLLENCAGCKNPLTTDNQLRIEPDCRSANPCYPGVECLDSAAGPRCGHCPLGFIGDGKSCKPGVTCAHHMCYPGVQCHDTVNGAQCDSCPAGYEGDGRTCSLRNPCLDTPCPSGAQCLQVGYPPYFHCISCPMGHEVNGTSCRDMNECLLYDPCDELATCTNLSPGFQCSPCPVGFDGTHAHGYFADYYSVDYRRQTCLDVDECRTGFFRCPEHSTCINEIGSYRCQCHEGYVTNGTYSCLDRSSVFMCPDGTVCDRNAVCLRMDNIRHKCHCNVGWAGNGLICGRDTDVDGWPDQAIGCPELRCQRDNCPKLPNSGQEDADLDGHGDGCDDDADGDNVQNSQDNCWLAYNTEQLDSDGDKVGDVCDNCVLKYNPRQLDTDEDGLGDECDGDIDNDSIPNALDNCPLLPNPSQSDVDNDGVGDACDNCPNLPNPDQKDRDMDFVGDACHRDIDGDDDGVPNSLDNCPMVSNSDQLDTDGDGTGDECDDDMDGDGIPNYKDNCPLAKNPKQDDFNRNGKGDSCEDDEDVDGVPNGMDNCPNNSMIHHTDFRTLQTIPLDPKGLSQADPNWVVHANGTEIVQTLNSDPGLAVGKDAFGGVDFDGTFYINDDTDDDYAGFVFSYQSSYKYYVVQWKKGTQTYWEPRPFTASAAPGIQIKLVNSTEGPGPMMRNSLWHEGNTDGEARLLWKDPKNIAWKERTSYRWSLVHRPAIGLIRLQMHEGNRLIFDSGNVFDSTLKGGRLGVFCFSQRMIIWSNLQYKCNNRVKPLIYNDLSDYLKTKVELQD

Target ORF information:

RefSeq Version	NM_078771.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thrombospondin, transcript variant A (Tsp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078771.3

ORF Insert Sequence:

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCGCCCAGT GCCTCCAGGT TGGCTATCCG 
CCCTACTTTC ACTGCATCTC GTGTCCAATG GGCCACGAGG TGAACGGAAC CAGCTGCCGG 
GATATGAATG AGTGCCTGCT CTACGATCCT TGCGACGAGC TGGCGACGTG CACCAATCTT 
AGTCCTGGCT TCCAGTGCAG CCCGTGTCCC GTTGGCTTCG ATGGCACCCA CGCCCATGGC 
TACTTCGCCG ACTACTACTC CGTGGATTAT CGCAGGCAAA CGTGCCTGGA CGTCGATGAG 
TGCCGCACTG GGTTCTTCCG ATGCCCGGAG CATTCTACTT GCATCAACGA GATTGGTTCC 
TACAGATGCC AATGCCACGA AGGATATGTA ACAAATGGCA CCTACAGCTG CCTAGACAGA 
TCCTCGGTGT TCATGTGTCC GGATGGAACC GTTTGTGACC GCAATGCCGT GTGCCTTAGG 
ATGGATAACA TCCGCCACAA GTGTCACTGC AATGTGGGAT GGGCGGGCAA CGGTTTGATT 
TGCGGTAGGG ATACCGACGT AGATGGATGG CCAGATCAGG CAATCGGTTG TCCAGAGCTG 
CGCTGTCAGC GAGACAACTG CCCGAAGTTG CCTAATTCAG GCCAGGAGGA CGCCGATCTG 
GATGGACATG GCGATGGGTG CGACGACGAT GCCGATGGCG ACAATGTCCA GAACAGCCAA 
GACAACTGCT GGCTGGCCTA CAACACCGAG CAGCTGGACT CGGATGGAGA CAAGGTGGGC 
GATGTGTGCG ACAACTGCGT GCTTAAGTAC AATCCCAGGC AGTTGGACAC GGACGAGGAT 
GGACTGGGAG ACGAGTGCGA TGGTGATATC GACAACGACT CCATACCAAA TGCGCTAGAC 
AACTGTCCGC TGCTGCCGAA TCCCAGTCAG TCGGACGTGG ACAACGATGG TGTGGGCGAC 
GCTTGCGATA ACTGTCCGAA TCTTCCGAAT CCCGACCAAA AGGATCGCGA CATGGACTTT 
GTAGGCGATG CCTGTCACAG AGACATCGAC GGGGATGATG ATGGAGTTCC AAACTCGCTG 
GACAACTGCC CAATGGTCAG TAACTCGGAC CAGCTGGACA CTGACGGTGA TGGAACGGGC 
GATGAGTGCG ATGACGACAT GGACGGAGAT GGGATACCCA ACTACAAGGA CAACTGTCCG 
CTGGCCAAGA ACCCAAAGCA GGACGACTTC AATCGGAATG GCAAGGGTGA CTCTTGCGAA 
GATGATGAGG ACGTAGATGG TGTACCGAAT GGCATGGACA ACTGCCCGAA CAACTCGATG 
ATTCATCACA CTGACTTTCG CACTCTTCAA ACCATTCCAC TGGATCCCAA AGGCCTATCG 
CAAGCGGATC CCAACTGGGT GGTACACGCC AATGGCACCG AGATAGTCCA GACCCTCAAT 
TCGGATCCGG GACTAGCGGT GGGCAAGGAC GCCTTCGGAG GCGTTGACTT CGACGGCACC 
TTTTATATCA ACGATGACAC GGACGATGAC TACGCTGGCT TTGTGTTCAG CTACCAGAGC 
AGCTACAAGT ACTACGTGGT GCAGTGGAAG AAGGGCACCC AAACCTACTG GGAGCCACGA 
CCCTTCACCG CCTCTGCGGC GCCGGGCATC CAGATCAAGC TGGTCAACAG CACCGAGGGT 
CCGGGCCCAA TGATGAGGAA CAGCCTGTGG CACGAGGGAA ACACCGACGG TGAGGCCAGG 
CTGCTGTGGA AGGATCCGAA GAACATTGCG TGGAAGGAAA GGACCTCCTA CCGCTGGTCC 
CTGGTGCATC GACCGGCCAT CGGCCTCATC CGCCTGCAAA TGCACGAGGG TAACCGCCTG 
ATCTTCGACT CGGGCAATGT GTTTGACTCC ACGCTGAAGG GCGGTCGGCT GGGCGTGTTC 
TGCTTCTCAC AGAGGATGAT TATATGGTCC AACCTGCAGT ACAAGTGCAA TAATCGTGTG 
AAGCCGCTGA TCTACAATGA TCTGTCCGAC TATCTCAAGA CGAAGGTGGA GTTGCAGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tsp ( NM_001144330.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137802.1 Drosophila melanogaster thrombospondin, transcript variant F (Tsp), mRNA.

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CloneID	OFa13950
Clone ID Related Accession (Same CDS sequence)	NM_078771.3 , NM_001144330.2
Accession Version	NM_001144330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thrombospondin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001144330.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCGCCCAGT GCCTCCAGGT TGGCTATCCG 
CCCTACTTTC ACTGCATCTC GTGTCCAATG GGCCACGAGG TGAACGGAAC CAGCTGCCGG 
GATATGAATG AGTGCCTGCT CTACGATCCT TGCGACGAGC TGGCGACGTG CACCAATCTT 
AGTCCTGGCT TCCAGTGCAG CCCGTGTCCC GTTGGCTTCG ATGGCACCCA CGCCCATGGC 
TACTTCGCCG ACTACTACTC CGTGGATTAT CGCAGGCAAA CGTGCCTGGA CGTCGATGAG 
TGCCGCACTG GGTTCTTCCG ATGCCCGGAG CATTCTACTT GCATCAACGA GATTGGTTCC 
TACAGATGCC AATGCCACGA AGGATATGTA ACAAATGGCA CCTACAGCTG CCTAGACAGA 
TCCTCGGTGT TCATGTGTCC GGATGGAACC GTTTGTGACC GCAATGCCGT GTGCCTTAGG 
ATGGATAACA TCCGCCACAA GTGTCACTGC AATGTGGGAT GGGCGGGCAA CGGTTTGATT 
TGCGGTAGGG ATACCGACGT AGATGGATGG CCAGATCAGG CAATCGGTTG TCCAGAGCTG 
CGCTGTCAGC GAGACAACTG CCCGAAGTTG CCTAATTCAG GCCAGGAGGA CGCCGATCTG 
GATGGACATG GCGATGGGTG CGACGACGAT GCCGATGGCG ACAATGTCCA GAACAGCCAA 
GACAACTGCT GGCTGGCCTA CAACACCGAG CAGCTGGACT CGGATGGAGA CAAGGTGGGC 
GATGTGTGCG ACAACTGCGT GCTTAAGTAC AATCCCAGGC AGTTGGACAC GGACGAGGAT 
GGACTGGGAG ACGAGTGCGA TGGTGATATC GACAACGACT CCATACCAAA TGCGCTAGAC 
AACTGTCCGC TGCTGCCGAA TCCCAGTCAG TCGGACGTGG ACAACGATGG TGTGGGCGAC 
GCTTGCGATA ACTGTCCGAA TCTTCCGAAT CCCGACCAAA AGGATCGCGA CATGGACTTT 
GTAGGCGATG CCTGTCACAG AGACATCGAC GGGGATGATG ATGGAGTTCC AAACTCGCTG 
GACAACTGCC CAATGGTCAG TAACTCGGAC CAGCTGGACA CTGACGGTGA TGGAACGGGC 
GATGAGTGCG ATGACGACAT GGACGGAGAT GGGATACCCA ACTACAAGGA CAACTGTCCG 
CTGGCCAAGA ACCCAAAGCA GGACGACTTC AATCGGAATG GCAAGGGTGA CTCTTGCGAA 
GATGATGAGG ACGTAGATGG TGTACCGAAT GGCATGGACA ACTGCCCGAA CAACTCGATG 
ATTCATCACA CTGACTTTCG CACTCTTCAA ACCATTCCAC TGGATCCCAA AGGCCTATCG 
CAAGCGGATC CCAACTGGGT GGTACACGCC AATGGCACCG AGATAGTCCA GACCCTCAAT 
TCGGATCCGG GACTAGCGGT GGGCAAGGAC GCCTTCGGAG GCGTTGACTT CGACGGCACC 
TTTTATATCA ACGATGACAC GGACGATGAC TACGCTGGCT TTGTGTTCAG CTACCAGAGC 
AGCTACAAGT ACTACGTGGT GCAGTGGAAG AAGGGCACCC AAACCTACTG GGAGCCACGA 
CCCTTCACCG CCTCTGCGGC GCCGGGCATC CAGATCAAGC TGGTCAACAG CACCGAGGGT 
CCGGGCCCAA TGATGAGGAA CAGCCTGTGG CACGAGGGAA ACACCGACGG TGAGGCCAGG 
CTGCTGTGGA AGGATCCGAA GAACATTGCG TGGAAGGAAA GGACCTCCTA CCGCTGGTCC 
CTGGTGCATC GACCGGCCAT CGGCCTCATC CGCCTGCAAA TGCACGAGGG TAACCGCCTG 
ATCTTCGACT CGGGCAATGT GTTTGACTCC ACGCTGAAGG GCGGTCGGCT GGGCGTGTTC 
TGCTTCTCAC AGAGGATGAT TATATGGTCC AACCTGCAGT ACAAGTGCAA TAATCGTGTG 
AAGCCGCTGA TCTACAATGA TCTGTCCGAC TATCTCAAGA CGAAGGTGGA GTTGCAGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137802.1
CDS	576..3758
Translation	MNWTRVLLIGLTALALTFVDVASLSLDPVASAELEQFIRKGDVVISTRHIRPRRKLHISIEALFMIDFPMLKHKMSFFLDRKQQRVTLDISANGATESRNFEIPNINETSTIRSLALQFSKNRITLHVDCKASTHHDIDMNLAKLYTQMDDPVIKLFRERKYPLHFDGDMEHSLQRANCQKGNHRRGNRRMLRNKITEREKNKKRDVRGWYEPTIAREGVVDHRHQEVPTDVERGDIPVLNGDCEDALARSLSDLLALVKLLREDVAHQRQEIAYLRMLLENCAGCKNPLTTDNQLRIEPDCRSANPCYPGVECLDSAAGPRCGHCPLGFIGDGKSCKPGVTCAHHMCYPGVQCHDTVNGAQCDSCPAGYEGDGRTCSLRNPCLDTPCPSGAQCLQVGYPPYFHCISCPMGHEVNGTSCRDMNECLLYDPCDELATCTNLSPGFQCSPCPVGFDGTHAHGYFADYYSVDYRRQTCLDVDECRTGFFRCPEHSTCINEIGSYRCQCHEGYVTNGTYSCLDRSSVFMCPDGTVCDRNAVCLRMDNIRHKCHCNVGWAGNGLICGRDTDVDGWPDQAIGCPELRCQRDNCPKLPNSGQEDADLDGHGDGCDDDADGDNVQNSQDNCWLAYNTEQLDSDGDKVGDVCDNCVLKYNPRQLDTDEDGLGDECDGDIDNDSIPNALDNCPLLPNPSQSDVDNDGVGDACDNCPNLPNPDQKDRDMDFVGDACHRDIDGDDDGVPNSLDNCPMVSNSDQLDTDGDGTGDECDDDMDGDGIPNYKDNCPLAKNPKQDDFNRNGKGDSCEDDEDVDGVPNGMDNCPNNSMIHHTDFRTLQTIPLDPKGLSQADPNWVVHANGTEIVQTLNSDPGLAVGKDAFGGVDFDGTFYINDDTDDDYAGFVFSYQSSYKYYVVQWKKGTQTYWEPRPFTASAAPGIQIKLVNSTEGPGPMMRNSLWHEGNTDGEARLLWKDPKNIAWKERTSYRWSLVHRPAIGLIRLQMHEGNRLIFDSGNVFDSTLKGGRLGVFCFSQRMIIWSNLQYKCNNRVKPLIYNDLSDYLKTKVELQD

Target ORF information:

RefSeq Version	NM_001144330.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thrombospondin, transcript variant F (Tsp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144330.2

ORF Insert Sequence:

ATGAATTGGA CGCGCGTGCT GTTAATCGGG TTGACCGCCT TGGCGCTGAC ATTTGTGGAT 
GTTGCATCAC TCTCACTCGA TCCAGTTGCT TCTGCTGAAC TGGAGCAGTT TATACGCAAA 
GGAGATGTGG TCATATCAAC GCGACACATT AGACCTCGTC GAAAGTTGCA CATATCCATA 
GAAGCACTCT TCATGATCGA CTTCCCGATG CTGAAGCACA AGATGTCCTT CTTCCTGGAC 
CGCAAACAGC AGCGGGTAAC GTTGGACATT AGTGCGAACG GAGCCACCGA GTCGCGCAAC 
TTCGAGATAC CCAACATCAA TGAGACAAGC ACCATCCGAT CCCTGGCACT GCAGTTCTCC 
AAGAATCGCA TAACGCTCCA TGTGGACTGC AAGGCGAGCA CGCATCACGA CATCGACATG 
AACCTGGCCA AGCTGTACAC CCAGATGGAC GATCCGGTGA TCAAGCTGTT CCGTGAGCGC 
AAGTATCCCC TTCACTTCGA CGGGGACATG GAGCACTCCC TGCAGCGGGC CAATTGCCAG 
AAGGGCAACC ATCGTCGTGG CAATCGTCGC ATGCTGCGCA ACAAGATCAC CGAGCGTGAG 
AAGAACAAGA AGCGCGATGT GCGCGGCTGG TATGAGCCAA CGATCGCCCG GGAAGGAGTC 
GTGGATCACA GACACCAGGA GGTGCCAACG GACGTGGAGC GCGGTGATAT TCCCGTTCTG 
AATGGCGATT GCGAAGACGC CCTCGCACGA TCGCTCAGCG ATTTACTGGC TCTGGTGAAG 
CTGCTCCGCG AAGACGTCGC CCACCAGCGC CAGGAGATTG CCTACCTGCG TATGCTCCTG 
GAGAACTGTG CCGGCTGCAA GAATCCCCTC ACCACCGACA ACCAACTGCG CATCGAGCCC 
GACTGCCGTT CCGCCAATCC CTGTTATCCT GGAGTGGAGT GCTTGGACTC GGCGGCCGGT 
CCCCGATGTG GCCACTGTCC CCTTGGCTTC ATTGGCGATG GCAAGAGCTG CAAGCCGGGC 
GTTACCTGCG CCCATCACAT GTGCTATCCA GGCGTTCAGT GTCACGATAC CGTGAATGGA 
GCCCAGTGTG ACTCCTGTCC AGCCGGCTAC GAAGGTGATG GACGCACATG TTCGCTACGT 
AATCCTTGCC TGGACACACC GTGCCCCTCA GGCGCCCAGT GCCTCCAGGT TGGCTATCCG 
CCCTACTTTC ACTGCATCTC GTGTCCAATG GGCCACGAGG TGAACGGAAC CAGCTGCCGG 
GATATGAATG AGTGCCTGCT CTACGATCCT TGCGACGAGC TGGCGACGTG CACCAATCTT 
AGTCCTGGCT TCCAGTGCAG CCCGTGTCCC GTTGGCTTCG ATGGCACCCA CGCCCATGGC 
TACTTCGCCG ACTACTACTC CGTGGATTAT CGCAGGCAAA CGTGCCTGGA CGTCGATGAG 
TGCCGCACTG GGTTCTTCCG ATGCCCGGAG CATTCTACTT GCATCAACGA GATTGGTTCC 
TACAGATGCC AATGCCACGA AGGATATGTA ACAAATGGCA CCTACAGCTG CCTAGACAGA 
TCCTCGGTGT TCATGTGTCC GGATGGAACC GTTTGTGACC GCAATGCCGT GTGCCTTAGG 
ATGGATAACA TCCGCCACAA GTGTCACTGC AATGTGGGAT GGGCGGGCAA CGGTTTGATT 
TGCGGTAGGG ATACCGACGT AGATGGATGG CCAGATCAGG CAATCGGTTG TCCAGAGCTG 
CGCTGTCAGC GAGACAACTG CCCGAAGTTG CCTAATTCAG GCCAGGAGGA CGCCGATCTG 
GATGGACATG GCGATGGGTG CGACGACGAT GCCGATGGCG ACAATGTCCA GAACAGCCAA 
GACAACTGCT GGCTGGCCTA CAACACCGAG CAGCTGGACT CGGATGGAGA CAAGGTGGGC 
GATGTGTGCG ACAACTGCGT GCTTAAGTAC AATCCCAGGC AGTTGGACAC GGACGAGGAT 
GGACTGGGAG ACGAGTGCGA TGGTGATATC GACAACGACT CCATACCAAA TGCGCTAGAC 
AACTGTCCGC TGCTGCCGAA TCCCAGTCAG TCGGACGTGG ACAACGATGG TGTGGGCGAC 
GCTTGCGATA ACTGTCCGAA TCTTCCGAAT CCCGACCAAA AGGATCGCGA CATGGACTTT 
GTAGGCGATG CCTGTCACAG AGACATCGAC GGGGATGATG ATGGAGTTCC AAACTCGCTG 
GACAACTGCC CAATGGTCAG TAACTCGGAC CAGCTGGACA CTGACGGTGA TGGAACGGGC 
GATGAGTGCG ATGACGACAT GGACGGAGAT GGGATACCCA ACTACAAGGA CAACTGTCCG 
CTGGCCAAGA ACCCAAAGCA GGACGACTTC AATCGGAATG GCAAGGGTGA CTCTTGCGAA 
GATGATGAGG ACGTAGATGG TGTACCGAAT GGCATGGACA ACTGCCCGAA CAACTCGATG 
ATTCATCACA CTGACTTTCG CACTCTTCAA ACCATTCCAC TGGATCCCAA AGGCCTATCG 
CAAGCGGATC CCAACTGGGT GGTACACGCC AATGGCACCG AGATAGTCCA GACCCTCAAT 
TCGGATCCGG GACTAGCGGT GGGCAAGGAC GCCTTCGGAG GCGTTGACTT CGACGGCACC 
TTTTATATCA ACGATGACAC GGACGATGAC TACGCTGGCT TTGTGTTCAG CTACCAGAGC 
AGCTACAAGT ACTACGTGGT GCAGTGGAAG AAGGGCACCC AAACCTACTG GGAGCCACGA 
CCCTTCACCG CCTCTGCGGC GCCGGGCATC CAGATCAAGC TGGTCAACAG CACCGAGGGT 
CCGGGCCCAA TGATGAGGAA CAGCCTGTGG CACGAGGGAA ACACCGACGG TGAGGCCAGG 
CTGCTGTGGA AGGATCCGAA GAACATTGCG TGGAAGGAAA GGACCTCCTA CCGCTGGTCC 
CTGGTGCATC GACCGGCCAT CGGCCTCATC CGCCTGCAAA TGCACGAGGG TAACCGCCTG 
ATCTTCGACT CGGGCAATGT GTTTGACTCC ACGCTGAAGG GCGGTCGGCT GGGCGTGTTC 
TGCTTCTCAC AGAGGATGAT TATATGGTCC AACCTGCAGT ACAAGTGCAA TAATCGTGTG 
AAGCCGCTGA TCTACAATGA TCTGTCCGAC TATCTCAAGA CGAAGGTGGA GTTGCAGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tsp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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