slam cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	slam
Entrez Gene ID	33890
Full Name	slow as molasses
Synonyms	CG9506,Dmel\CG9506,Slam,wdo
General protein information	Preferred Names slow as molasses Names CG9506 gene product from transcript CG9506-RB CG9506-PB CG9506-PC slam-PB slam-PC waldo
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26C4-26C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135176.3	NP_609020.3	slow as molasses, isoform B
NM_001298739.1	NP_001285668.1	slow as molasses, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Biochemical interaction of slam mRNA and protein as demonstrated by specific co-immunoprecipitation from embryonic lysate. The intimate relationship of slam mRNA and protein may constitute a positive feedback loop that facilitates and controls timely and rapid accumulation of Slam protein at the prospective basal region.
Title: Slam protein dictates subcellular localization and translation of its own mRNA.

Following the onset of zygotic gene expression, Slam and Nullo together stabilise the retracting metaphase furrow and Slam triggers invagination of the cleavage furrow.
Title: Function and dynamics of slam in furrow formation in early Drosophila embryo.

Accumulation of slam mRNA and protein at the presumptive invagination site provides a spatial and temporal trigger for RhoGEF2-Rho1 signalling.
Title: Localization of RhoGEF2 during Drosophila cellularization is developmentally controlled by Slam.

analysis of the slow as molasses (slam) gene provides a potential link between somatic cell formation and germ cell migration
Title: Germ cell migration: as slow as molasses.

Slam is required for the localization of a signal to the basal side of blastoderm cells that is needed later in the posterior midgut to guide germ cells.
Title: Slow as molasses is required for polarized membrane growth and germ cell migration in Drosophila.

The following slam gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slam cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13902	NM_001298739.1 Latest version!	Drosophila melanogaster slow as molasses (slam), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa13902	NM_135176.3 Latest version!	Drosophila melanogaster slow as molasses (slam), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slam ( NM_135176.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609020.3 Drosophila melanogaster slow as molasses (slam), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13902
Clone ID Related Accession (Same CDS sequence)	NM_135176.3 , NM_001298739.1
Accession Version	NM_135176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slow as molasses, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135176.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCCTAA GCAATTCCAC GCCGAACAAC CAGTCCAAGC ACAACCAGAT GGTAGTGGAC 
ACGGCAGCCA TGAACAGTGA TGATCTCAGC GAGCTACTGC AGCTGAACGC GGAGATCGAG 
GAACGTCGTC GCTCCAGCCG CCACGGAGAT GCCAGCACCG CCTGTGGCCT GCTCCGAGCT 
ACGATGACCA GGGAGGAACT CTTCGAGATT TCCTCGCTGG ATGATGATCG CTTCCTGACC 
GCCCTGGAGT ACCAAAACTC GTTTGCATCA CCGCGCCGAG TGCAGGTTAC CGACCTGGAC 
TTGTCCAGCA TCGAGAATCT GATGAAGTAC TTCGACGAAG AGGTACCTGT GACGCCAACA 
AAGACACTGG GTACCACCAA GGCAGCGGGT AACACGGGTA AAGTGGCCAG CACAATTGCC 
AAGTTGGCCC TCCAGTCGGA TCCGGTCACG CCGCCCAAGC CAAAAGTGGG TAGTGGCCAC 
CTAAAGATCA GCGAGCTCAA GCAGAAATAC GAACAGCTGC CGGAGATGGA GACGCCACGC 
TCTGCATATC AGGCTTCCAG AAAGGTATCC GCGTCGCTGC CCATGAAGGT CAAGGAGATG 
GCCCAGCTGT TTAACTCCAA GATCAGCCAG GTGATGCGAC GTACAGAGGA ACCACAGTAT 
GTTCAGCTGC AAAATGAGAT GTCGCCGGAG GTTAAGGCAC AGAACCGCCT GGTGTCGCCA 
TTAGAGTCCC CAGTTCAAGG ACCCTGCCTG GTGGCCGAGG AAGTTTTCCG CGAGTTGAGC 
GTGAAGGACA AAGCCCTGCT GTTCAACAAG TTCATCGGTG ACATGGCAGC CAAGCACCCC 
AAGTTCACTG CACATGCCGC AGATCTCAAG GAGAAGGTAA ACAAACAGGT GGCACGAGGG 
GAGGTGGTGG CTGAACGACA AGCCAGTGTT AAGCATCTCG CCCAGGAGTT GGAAGCGAAA 
TGTATCCTGG AGCCGGGATC GCCACCGCGA CCAGGTGGTG TCTCTCCCCC CAAGACGACT 
GAGAGCAAAA CGGAGACATG CACCTCCCAG CTGCATGTGA GCACCCTGAC TGTGATCCTC 
AAGCCAAGTC CAGAGCGCAG AGCTCCACAG CCACGACCCC GCCGATGCCT GGATCGTCAG 
AGCGATGAAC ATGCCCGAGA GCCATCCCAG AAGCGAAACC TCGCCGCGAT TCGCACCATG 
ATGCCAACGG AGGCCTATGC GCCACCAAAG AAAATCCGAC GCACTCGACA GGAGCGCACC 
GGTGCGGATA GCCAAATGTT TTTTCAGAAC GAACACCTCG AAACCCTGTT CTACAGCTGG 
CTCAGCTCGG AGAACGGTGT CCAGTTCGAC ATTACGAGTG TGTCTGACGG TCAGCAGACC 
ATTGAGATAG CCACCGAGGA GGGTAATCTT CTGGAGCAAC CGCTACTTGA GCCGAGCACC 
GCTACTCTGG AGGATGTGAG CCAAAAGTCC GCCGTCGAGC GGCTTCTGGA GGAGGCCATC 
GCAAAATTAG AGCTAGATAA CGAGTCCAAG AAGGAGACAC AAGTCGAGGA AAAGAAGGAG 
ATGGATACAG CTCAGGATCC CATCGTTCAA GTCACTCCGC GACGCATTAA GCGCCAGGCG 
CCGCCAGTGC CAGCCCCACG ACCCAGTCTA AGTCAAGTTA CCTCCAGTGC ATCCAGCTGC 
AAGCAATCCG AGGCTGAGGA AAGTGAATCC GGGTTGTCTA CACTCCCCAA GATAACCAGC 
GACGAATCCC AGCCAGAGAC GCCCAAGGAT GACTTGCAGA CAGGTGAATT TGATTTTGCC 
AAGCCCCAGC GTCCGCCACG CAAGAAGAAA ATGCGCCGAA CTCTTACATG GAAAAAGGAG 
AACTCCATTG TCGAGGCCAC TGCAATAACC TCAACGGACT CCGATTCAGA TTACAAGCCT 
CCGCTATCAG GAGCGGCCAA GAAGAAGTCC GGTGCTATCT GTAACCCACT TCCAATGCCC 
GAGCCAAGTT TCATTGAGTT GGATAAGTCA CTGATGCGCC ACTTGAACTC GCCCCGGAAG 
ATCAAGTCGG CATACACCCT CACCGTGATG TCATCTCCCT CGCCGAATGC AGACAGCGAT 
CAGTCTCCTA GTCAGACACC AAGGCAATCC CTGGTACAGG CCATGCGGGA CAGTTTCGTG 
GATCAGGGCT TTGAGACATG CTCGAACGAT CCGATGGACA ACAGTCCGAT ACGCCGATCT 
TCGCTTGGAG CCACGGAATC GAAGCCATCC GGTTTTTCCA CGCCAGTTAA GGGACGCCAT 
GCTTCCAGTC CGGCGCAACA ACAACTGTTC AGTCCGATTC TCATCCAGGA GCGCCCTCGT 
CGCAGTTCCC TGGCCATGCA AGTGATCCGG GAGGATCATC CGCTCGATCT GGACGCCACC 
TCCAGCTCAC CCTCCACACC GTGCAGTGAG CGGGAGTTCT TCGCCAATGC CCCGACTGTT 
GAAATTGATA ACTCCCAGGA TGAGAGTCCG ACCGGCAAGG CGCACTCAAT GTTCTGGATC 
ACCTCTGGGG ACTTCACAGT CTCCCTGGAA ATCTTCAAGA ACAGCCCGGA GCGCCTGCGA 
CTGCTGTACG AAATCTTCAC CCAGAAAAGC TGGGAGACTC GTGACCTGGC CTTCGGAATC 
GACGGACACA AGTTCATTCG CGGAGCTCCC AGTTCCGACT CCGTCCGACA GTCGCTGCCC 
GAGCGTCCAC CCAGTGTGAA GGGCTGCTCT CACTATTGGT TCGCCAGCGG AGATCTCGCA 
GTGCCCTTCA GCGGAAAACT GATGTCCAGC GAGAAGATCG AGCGGCTGTT TGCCTTCCTC 
AGCGGAGAGC AGTCGGAGTT GCGCTTCGGC GTTGACCACA TTGAGTTCAG CAGCGTGCCT 
GAGTTCTGGC CCACCACCCA GAAGTATTCC ATCGAGAGCA GCTACAGCAT ACTGGTGGGT 
CTGCAGACAG GTGCCAGCAA TGGCCTGGAG GGTCGCAGCA AGTACTCCTG GCCCAACAGC 
AGCATCAGTG CCAACCAGGC GATCAAGACC AGCGACCTGG ATCAGACGGA GTTCGAGTCC 
GATAGCTTCG GCAACAACAG TGGTAGGCTG TCCTTTTCGC CTGATCTCTT CTCCCTGGAC 
TACGAGGCGG TTCCCCTGGA CGAGCTGTTT GCCAAGGCTC CTCCCTCTGC CGCCACACCC 
GCCATGTCCG TGCCGCAAAT GATGCAGACC CTCAAGCAGC AGCAGTCCAA ACTTCGCAGT 
GTGGAGCAGC GCATTCGTGG CTACGCCAAG CCCGCGAATC TGGCCGACTC TTCGCTGGAG 
CACTGCCGCA ACACGCCGCA ATATGTCCAC AAGCTGCGCT CCATCATCCG GGCCATTGAC 
AACATTGGAC GCGACGATGG CTTCCGCGGC TGCTCGATGG AGCAGCTCGA GAGCTTCATG 
TACTTCCTCA GCGAGTATGC GGATGTGTGC CTGGCCAACT GCAGCGAGCA CATGGACAAG 
ATTCTCGACA CCCTGATGGA CCGGAGGGCC GTGGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609020.3
CDS	476..3997
Translation	MVLSNSTPNNQSKHNQMVVDTAAMNSDDLSELLQLNAEIEERRRSSRHGDASTACGLLRATMTREELFEISSLDDDRFLTALEYQNSFASPRRVQVTDLDLSSIENLMKYFDEEVPVTPTKTLGTTKAAGNTGKVASTIAKLALQSDPVTPPKPKVGSGHLKISELKQKYEQLPEMETPRSAYQASRKVSASLPMKVKEMAQLFNSKISQVMRRTEEPQYVQLQNEMSPEVKAQNRLVSPLESPVQGPCLVAEEVFRELSVKDKALLFNKFIGDMAAKHPKFTAHAADLKEKVNKQVARGEVVAERQASVKHLAQELEAKCILEPGSPPRPGGVSPPKTTESKTETCTSQLHVSTLTVILKPSPERRAPQPRPRRCLDRQSDEHAREPSQKRNLAAIRTMMPTEAYAPPKKIRRTRQERTGADSQMFFQNEHLETLFYSWLSSENGVQFDITSVSDGQQTIEIATEEGNLLEQPLLEPSTATLEDVSQKSAVERLLEEAIAKLELDNESKKETQVEEKKEMDTAQDPIVQVTPRRIKRQAPPVPAPRPSLSQVTSSASSCKQSEAEESESGLSTLPKITSDESQPETPKDDLQTGEFDFAKPQRPPRKKKMRRTLTWKKENSIVEATAITSTDSDSDYKPPLSGAAKKKSGAICNPLPMPEPSFIELDKSLMRHLNSPRKIKSAYTLTVMSSPSPNADSDQSPSQTPRQSLVQAMRDSFVDQGFETCSNDPMDNSPIRRSSLGATESKPSGFSTPVKGRHASSPAQQQLFSPILIQERPRRSSLAMQVIREDHPLDLDATSSSPSTPCSEREFFANAPTVEIDNSQDESPTGKAHSMFWITSGDFTVSLEIFKNSPERLRLLYEIFTQKSWETRDLAFGIDGHKFIRGAPSSDSVRQSLPERPPSVKGCSHYWFASGDLAVPFSGKLMSSEKIERLFAFLSGEQSELRFGVDHIEFSSVPEFWPTTQKYSIESSYSILVGLQTGASNGLEGRSKYSWPNSSISANQAIKTSDLDQTEFESDSFGNNSGRLSFSPDLFSLDYEAVPLDELFAKAPPSAATPAMSVPQMMQTLKQQQSKLRSVEQRIRGYAKPANLADSSLEHCRNTPQYVHKLRSIIRAIDNIGRDDGFRGCSMEQLESFMYFLSEYADVCLANCSEHMDKILDTLMDRRAVEV

Target ORF information:

RefSeq Version	NM_135176.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slow as molasses (slam), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135176.3

ORF Insert Sequence:

ATGGTCCTAA GCAATTCCAC GCCGAACAAC CAGTCCAAGC ACAACCAGAT GGTAGTGGAC 
ACGGCAGCCA TGAACAGTGA TGATCTCAGC GAGCTACTGC AGCTGAACGC GGAGATCGAG 
GAACGTCGTC GCTCCAGCCG CCACGGAGAT GCCAGCACCG CCTGTGGCCT GCTCCGAGCT 
ACGATGACCA GGGAGGAACT CTTCGAGATT TCCTCGCTGG ATGATGATCG CTTCCTGACC 
GCCCTGGAGT ACCAAAACTC GTTTGCATCA CCGCGCCGAG TGCAGGTTAC CGACCTGGAC 
TTGTCCAGCA TCGAGAATCT GATGAAGTAC TTCGACGAAG AGGTACCTGT GACGCCAACA 
AAGACACTGG GTACCACCAA GGCAGCGGGT AACACGGGTA AAGTGGCCAG CACAATTGCC 
AAGTTGGCCC TCCAGTCGGA TCCGGTCACG CCGCCCAAGC CAAAAGTGGG TAGTGGCCAC 
CTAAAGATCA GCGAGCTCAA GCAGAAATAC GAACAGCTGC CGGAGATGGA GACGCCACGC 
TCTGCATATC AGGCTTCCAG AAAGGTATCC GCGTCGCTGC CCATGAAGGT CAAGGAGATG 
GCCCAGCTGT TTAACTCCAA GATCAGCCAG GTGATGCGAC GTACAGAGGA ACCACAGTAT 
GTTCAGCTGC AAAATGAGAT GTCGCCGGAG GTTAAGGCAC AGAACCGCCT GGTGTCGCCA 
TTAGAGTCCC CAGTTCAAGG ACCCTGCCTG GTGGCCGAGG AAGTTTTCCG CGAGTTGAGC 
GTGAAGGACA AAGCCCTGCT GTTCAACAAG TTCATCGGTG ACATGGCAGC CAAGCACCCC 
AAGTTCACTG CACATGCCGC AGATCTCAAG GAGAAGGTAA ACAAACAGGT GGCACGAGGG 
GAGGTGGTGG CTGAACGACA AGCCAGTGTT AAGCATCTCG CCCAGGAGTT GGAAGCGAAA 
TGTATCCTGG AGCCGGGATC GCCACCGCGA CCAGGTGGTG TCTCTCCCCC CAAGACGACT 
GAGAGCAAAA CGGAGACATG CACCTCCCAG CTGCATGTGA GCACCCTGAC TGTGATCCTC 
AAGCCAAGTC CAGAGCGCAG AGCTCCACAG CCACGACCCC GCCGATGCCT GGATCGTCAG 
AGCGATGAAC ATGCCCGAGA GCCATCCCAG AAGCGAAACC TCGCCGCGAT TCGCACCATG 
ATGCCAACGG AGGCCTATGC GCCACCAAAG AAAATCCGAC GCACTCGACA GGAGCGCACC 
GGTGCGGATA GCCAAATGTT TTTTCAGAAC GAACACCTCG AAACCCTGTT CTACAGCTGG 
CTCAGCTCGG AGAACGGTGT CCAGTTCGAC ATTACGAGTG TGTCTGACGG TCAGCAGACC 
ATTGAGATAG CCACCGAGGA GGGTAATCTT CTGGAGCAAC CGCTACTTGA GCCGAGCACC 
GCTACTCTGG AGGATGTGAG CCAAAAGTCC GCCGTCGAGC GGCTTCTGGA GGAGGCCATC 
GCAAAATTAG AGCTAGATAA CGAGTCCAAG AAGGAGACAC AAGTCGAGGA AAAGAAGGAG 
ATGGATACAG CTCAGGATCC CATCGTTCAA GTCACTCCGC GACGCATTAA GCGCCAGGCG 
CCGCCAGTGC CAGCCCCACG ACCCAGTCTA AGTCAAGTTA CCTCCAGTGC ATCCAGCTGC 
AAGCAATCCG AGGCTGAGGA AAGTGAATCC GGGTTGTCTA CACTCCCCAA GATAACCAGC 
GACGAATCCC AGCCAGAGAC GCCCAAGGAT GACTTGCAGA CAGGTGAATT TGATTTTGCC 
AAGCCCCAGC GTCCGCCACG CAAGAAGAAA ATGCGCCGAA CTCTTACATG GAAAAAGGAG 
AACTCCATTG TCGAGGCCAC TGCAATAACC TCAACGGACT CCGATTCAGA TTACAAGCCT 
CCGCTATCAG GAGCGGCCAA GAAGAAGTCC GGTGCTATCT GTAACCCACT TCCAATGCCC 
GAGCCAAGTT TCATTGAGTT GGATAAGTCA CTGATGCGCC ACTTGAACTC GCCCCGGAAG 
ATCAAGTCGG CATACACCCT CACCGTGATG TCATCTCCCT CGCCGAATGC AGACAGCGAT 
CAGTCTCCTA GTCAGACACC AAGGCAATCC CTGGTACAGG CCATGCGGGA CAGTTTCGTG 
GATCAGGGCT TTGAGACATG CTCGAACGAT CCGATGGACA ACAGTCCGAT ACGCCGATCT 
TCGCTTGGAG CCACGGAATC GAAGCCATCC GGTTTTTCCA CGCCAGTTAA GGGACGCCAT 
GCTTCCAGTC CGGCGCAACA ACAACTGTTC AGTCCGATTC TCATCCAGGA GCGCCCTCGT 
CGCAGTTCCC TGGCCATGCA AGTGATCCGG GAGGATCATC CGCTCGATCT GGACGCCACC 
TCCAGCTCAC CCTCCACACC GTGCAGTGAG CGGGAGTTCT TCGCCAATGC CCCGACTGTT 
GAAATTGATA ACTCCCAGGA TGAGAGTCCG ACCGGCAAGG CGCACTCAAT GTTCTGGATC 
ACCTCTGGGG ACTTCACAGT CTCCCTGGAA ATCTTCAAGA ACAGCCCGGA GCGCCTGCGA 
CTGCTGTACG AAATCTTCAC CCAGAAAAGC TGGGAGACTC GTGACCTGGC CTTCGGAATC 
GACGGACACA AGTTCATTCG CGGAGCTCCC AGTTCCGACT CCGTCCGACA GTCGCTGCCC 
GAGCGTCCAC CCAGTGTGAA GGGCTGCTCT CACTATTGGT TCGCCAGCGG AGATCTCGCA 
GTGCCCTTCA GCGGAAAACT GATGTCCAGC GAGAAGATCG AGCGGCTGTT TGCCTTCCTC 
AGCGGAGAGC AGTCGGAGTT GCGCTTCGGC GTTGACCACA TTGAGTTCAG CAGCGTGCCT 
GAGTTCTGGC CCACCACCCA GAAGTATTCC ATCGAGAGCA GCTACAGCAT ACTGGTGGGT 
CTGCAGACAG GTGCCAGCAA TGGCCTGGAG GGTCGCAGCA AGTACTCCTG GCCCAACAGC 
AGCATCAGTG CCAACCAGGC GATCAAGACC AGCGACCTGG ATCAGACGGA GTTCGAGTCC 
GATAGCTTCG GCAACAACAG TGGTAGGCTG TCCTTTTCGC CTGATCTCTT CTCCCTGGAC 
TACGAGGCGG TTCCCCTGGA CGAGCTGTTT GCCAAGGCTC CTCCCTCTGC CGCCACACCC 
GCCATGTCCG TGCCGCAAAT GATGCAGACC CTCAAGCAGC AGCAGTCCAA ACTTCGCAGT 
GTGGAGCAGC GCATTCGTGG CTACGCCAAG CCCGCGAATC TGGCCGACTC TTCGCTGGAG 
CACTGCCGCA ACACGCCGCA ATATGTCCAC AAGCTGCGCT CCATCATCCG GGCCATTGAC 
AACATTGGAC GCGACGATGG CTTCCGCGGC TGCTCGATGG AGCAGCTCGA GAGCTTCATG 
TACTTCCTCA GCGAGTATGC GGATGTGTGC CTGGCCAACT GCAGCGAGCA CATGGACAAG 
ATTCTCGACA CCCTGATGGA CCGGAGGGCC GTGGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slam ( NM_001298739.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285668.1 Drosophila melanogaster slow as molasses (slam), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13902
Clone ID Related Accession (Same CDS sequence)	NM_135176.3 , NM_001298739.1
Accession Version	NM_001298739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slow as molasses, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCCTAA GCAATTCCAC GCCGAACAAC CAGTCCAAGC ACAACCAGAT GGTAGTGGAC 
ACGGCAGCCA TGAACAGTGA TGATCTCAGC GAGCTACTGC AGCTGAACGC GGAGATCGAG 
GAACGTCGTC GCTCCAGCCG CCACGGAGAT GCCAGCACCG CCTGTGGCCT GCTCCGAGCT 
ACGATGACCA GGGAGGAACT CTTCGAGATT TCCTCGCTGG ATGATGATCG CTTCCTGACC 
GCCCTGGAGT ACCAAAACTC GTTTGCATCA CCGCGCCGAG TGCAGGTTAC CGACCTGGAC 
TTGTCCAGCA TCGAGAATCT GATGAAGTAC TTCGACGAAG AGGTACCTGT GACGCCAACA 
AAGACACTGG GTACCACCAA GGCAGCGGGT AACACGGGTA AAGTGGCCAG CACAATTGCC 
AAGTTGGCCC TCCAGTCGGA TCCGGTCACG CCGCCCAAGC CAAAAGTGGG TAGTGGCCAC 
CTAAAGATCA GCGAGCTCAA GCAGAAATAC GAACAGCTGC CGGAGATGGA GACGCCACGC 
TCTGCATATC AGGCTTCCAG AAAGGTATCC GCGTCGCTGC CCATGAAGGT CAAGGAGATG 
GCCCAGCTGT TTAACTCCAA GATCAGCCAG GTGATGCGAC GTACAGAGGA ACCACAGTAT 
GTTCAGCTGC AAAATGAGAT GTCGCCGGAG GTTAAGGCAC AGAACCGCCT GGTGTCGCCA 
TTAGAGTCCC CAGTTCAAGG ACCCTGCCTG GTGGCCGAGG AAGTTTTCCG CGAGTTGAGC 
GTGAAGGACA AAGCCCTGCT GTTCAACAAG TTCATCGGTG ACATGGCAGC CAAGCACCCC 
AAGTTCACTG CACATGCCGC AGATCTCAAG GAGAAGGTAA ACAAACAGGT GGCACGAGGG 
GAGGTGGTGG CTGAACGACA AGCCAGTGTT AAGCATCTCG CCCAGGAGTT GGAAGCGAAA 
TGTATCCTGG AGCCGGGATC GCCACCGCGA CCAGGTGGTG TCTCTCCCCC CAAGACGACT 
GAGAGCAAAA CGGAGACATG CACCTCCCAG CTGCATGTGA GCACCCTGAC TGTGATCCTC 
AAGCCAAGTC CAGAGCGCAG AGCTCCACAG CCACGACCCC GCCGATGCCT GGATCGTCAG 
AGCGATGAAC ATGCCCGAGA GCCATCCCAG AAGCGAAACC TCGCCGCGAT TCGCACCATG 
ATGCCAACGG AGGCCTATGC GCCACCAAAG AAAATCCGAC GCACTCGACA GGAGCGCACC 
GGTGCGGATA GCCAAATGTT TTTTCAGAAC GAACACCTCG AAACCCTGTT CTACAGCTGG 
CTCAGCTCGG AGAACGGTGT CCAGTTCGAC ATTACGAGTG TGTCTGACGG TCAGCAGACC 
ATTGAGATAG CCACCGAGGA GGGTAATCTT CTGGAGCAAC CGCTACTTGA GCCGAGCACC 
GCTACTCTGG AGGATGTGAG CCAAAAGTCC GCCGTCGAGC GGCTTCTGGA GGAGGCCATC 
GCAAAATTAG AGCTAGATAA CGAGTCCAAG AAGGAGACAC AAGTCGAGGA AAAGAAGGAG 
ATGGATACAG CTCAGGATCC CATCGTTCAA GTCACTCCGC GACGCATTAA GCGCCAGGCG 
CCGCCAGTGC CAGCCCCACG ACCCAGTCTA AGTCAAGTTA CCTCCAGTGC ATCCAGCTGC 
AAGCAATCCG AGGCTGAGGA AAGTGAATCC GGGTTGTCTA CACTCCCCAA GATAACCAGC 
GACGAATCCC AGCCAGAGAC GCCCAAGGAT GACTTGCAGA CAGGTGAATT TGATTTTGCC 
AAGCCCCAGC GTCCGCCACG CAAGAAGAAA ATGCGCCGAA CTCTTACATG GAAAAAGGAG 
AACTCCATTG TCGAGGCCAC TGCAATAACC TCAACGGACT CCGATTCAGA TTACAAGCCT 
CCGCTATCAG GAGCGGCCAA GAAGAAGTCC GGTGCTATCT GTAACCCACT TCCAATGCCC 
GAGCCAAGTT TCATTGAGTT GGATAAGTCA CTGATGCGCC ACTTGAACTC GCCCCGGAAG 
ATCAAGTCGG CATACACCCT CACCGTGATG TCATCTCCCT CGCCGAATGC AGACAGCGAT 
CAGTCTCCTA GTCAGACACC AAGGCAATCC CTGGTACAGG CCATGCGGGA CAGTTTCGTG 
GATCAGGGCT TTGAGACATG CTCGAACGAT CCGATGGACA ACAGTCCGAT ACGCCGATCT 
TCGCTTGGAG CCACGGAATC GAAGCCATCC GGTTTTTCCA CGCCAGTTAA GGGACGCCAT 
GCTTCCAGTC CGGCGCAACA ACAACTGTTC AGTCCGATTC TCATCCAGGA GCGCCCTCGT 
CGCAGTTCCC TGGCCATGCA AGTGATCCGG GAGGATCATC CGCTCGATCT GGACGCCACC 
TCCAGCTCAC CCTCCACACC GTGCAGTGAG CGGGAGTTCT TCGCCAATGC CCCGACTGTT 
GAAATTGATA ACTCCCAGGA TGAGAGTCCG ACCGGCAAGG CGCACTCAAT GTTCTGGATC 
ACCTCTGGGG ACTTCACAGT CTCCCTGGAA ATCTTCAAGA ACAGCCCGGA GCGCCTGCGA 
CTGCTGTACG AAATCTTCAC CCAGAAAAGC TGGGAGACTC GTGACCTGGC CTTCGGAATC 
GACGGACACA AGTTCATTCG CGGAGCTCCC AGTTCCGACT CCGTCCGACA GTCGCTGCCC 
GAGCGTCCAC CCAGTGTGAA GGGCTGCTCT CACTATTGGT TCGCCAGCGG AGATCTCGCA 
GTGCCCTTCA GCGGAAAACT GATGTCCAGC GAGAAGATCG AGCGGCTGTT TGCCTTCCTC 
AGCGGAGAGC AGTCGGAGTT GCGCTTCGGC GTTGACCACA TTGAGTTCAG CAGCGTGCCT 
GAGTTCTGGC CCACCACCCA GAAGTATTCC ATCGAGAGCA GCTACAGCAT ACTGGTGGGT 
CTGCAGACAG GTGCCAGCAA TGGCCTGGAG GGTCGCAGCA AGTACTCCTG GCCCAACAGC 
AGCATCAGTG CCAACCAGGC GATCAAGACC AGCGACCTGG ATCAGACGGA GTTCGAGTCC 
GATAGCTTCG GCAACAACAG TGGTAGGCTG TCCTTTTCGC CTGATCTCTT CTCCCTGGAC 
TACGAGGCGG TTCCCCTGGA CGAGCTGTTT GCCAAGGCTC CTCCCTCTGC CGCCACACCC 
GCCATGTCCG TGCCGCAAAT GATGCAGACC CTCAAGCAGC AGCAGTCCAA ACTTCGCAGT 
GTGGAGCAGC GCATTCGTGG CTACGCCAAG CCCGCGAATC TGGCCGACTC TTCGCTGGAG 
CACTGCCGCA ACACGCCGCA ATATGTCCAC AAGCTGCGCT CCATCATCCG GGCCATTGAC 
AACATTGGAC GCGACGATGG CTTCCGCGGC TGCTCGATGG AGCAGCTCGA GAGCTTCATG 
TACTTCCTCA GCGAGTATGC GGATGTGTGC CTGGCCAACT GCAGCGAGCA CATGGACAAG 
ATTCTCGACA CCCTGATGGA CCGGAGGGCC GTGGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285668.1
CDS	63..3584
Translation	MVLSNSTPNNQSKHNQMVVDTAAMNSDDLSELLQLNAEIEERRRSSRHGDASTACGLLRATMTREELFEISSLDDDRFLTALEYQNSFASPRRVQVTDLDLSSIENLMKYFDEEVPVTPTKTLGTTKAAGNTGKVASTIAKLALQSDPVTPPKPKVGSGHLKISELKQKYEQLPEMETPRSAYQASRKVSASLPMKVKEMAQLFNSKISQVMRRTEEPQYVQLQNEMSPEVKAQNRLVSPLESPVQGPCLVAEEVFRELSVKDKALLFNKFIGDMAAKHPKFTAHAADLKEKVNKQVARGEVVAERQASVKHLAQELEAKCILEPGSPPRPGGVSPPKTTESKTETCTSQLHVSTLTVILKPSPERRAPQPRPRRCLDRQSDEHAREPSQKRNLAAIRTMMPTEAYAPPKKIRRTRQERTGADSQMFFQNEHLETLFYSWLSSENGVQFDITSVSDGQQTIEIATEEGNLLEQPLLEPSTATLEDVSQKSAVERLLEEAIAKLELDNESKKETQVEEKKEMDTAQDPIVQVTPRRIKRQAPPVPAPRPSLSQVTSSASSCKQSEAEESESGLSTLPKITSDESQPETPKDDLQTGEFDFAKPQRPPRKKKMRRTLTWKKENSIVEATAITSTDSDSDYKPPLSGAAKKKSGAICNPLPMPEPSFIELDKSLMRHLNSPRKIKSAYTLTVMSSPSPNADSDQSPSQTPRQSLVQAMRDSFVDQGFETCSNDPMDNSPIRRSSLGATESKPSGFSTPVKGRHASSPAQQQLFSPILIQERPRRSSLAMQVIREDHPLDLDATSSSPSTPCSEREFFANAPTVEIDNSQDESPTGKAHSMFWITSGDFTVSLEIFKNSPERLRLLYEIFTQKSWETRDLAFGIDGHKFIRGAPSSDSVRQSLPERPPSVKGCSHYWFASGDLAVPFSGKLMSSEKIERLFAFLSGEQSELRFGVDHIEFSSVPEFWPTTQKYSIESSYSILVGLQTGASNGLEGRSKYSWPNSSISANQAIKTSDLDQTEFESDSFGNNSGRLSFSPDLFSLDYEAVPLDELFAKAPPSAATPAMSVPQMMQTLKQQQSKLRSVEQRIRGYAKPANLADSSLEHCRNTPQYVHKLRSIIRAIDNIGRDDGFRGCSMEQLESFMYFLSEYADVCLANCSEHMDKILDTLMDRRAVEV

Target ORF information:

RefSeq Version	NM_001298739.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slow as molasses (slam), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298739.1

ORF Insert Sequence:

ATGGTCCTAA GCAATTCCAC GCCGAACAAC CAGTCCAAGC ACAACCAGAT GGTAGTGGAC 
ACGGCAGCCA TGAACAGTGA TGATCTCAGC GAGCTACTGC AGCTGAACGC GGAGATCGAG 
GAACGTCGTC GCTCCAGCCG CCACGGAGAT GCCAGCACCG CCTGTGGCCT GCTCCGAGCT 
ACGATGACCA GGGAGGAACT CTTCGAGATT TCCTCGCTGG ATGATGATCG CTTCCTGACC 
GCCCTGGAGT ACCAAAACTC GTTTGCATCA CCGCGCCGAG TGCAGGTTAC CGACCTGGAC 
TTGTCCAGCA TCGAGAATCT GATGAAGTAC TTCGACGAAG AGGTACCTGT GACGCCAACA 
AAGACACTGG GTACCACCAA GGCAGCGGGT AACACGGGTA AAGTGGCCAG CACAATTGCC 
AAGTTGGCCC TCCAGTCGGA TCCGGTCACG CCGCCCAAGC CAAAAGTGGG TAGTGGCCAC 
CTAAAGATCA GCGAGCTCAA GCAGAAATAC GAACAGCTGC CGGAGATGGA GACGCCACGC 
TCTGCATATC AGGCTTCCAG AAAGGTATCC GCGTCGCTGC CCATGAAGGT CAAGGAGATG 
GCCCAGCTGT TTAACTCCAA GATCAGCCAG GTGATGCGAC GTACAGAGGA ACCACAGTAT 
GTTCAGCTGC AAAATGAGAT GTCGCCGGAG GTTAAGGCAC AGAACCGCCT GGTGTCGCCA 
TTAGAGTCCC CAGTTCAAGG ACCCTGCCTG GTGGCCGAGG AAGTTTTCCG CGAGTTGAGC 
GTGAAGGACA AAGCCCTGCT GTTCAACAAG TTCATCGGTG ACATGGCAGC CAAGCACCCC 
AAGTTCACTG CACATGCCGC AGATCTCAAG GAGAAGGTAA ACAAACAGGT GGCACGAGGG 
GAGGTGGTGG CTGAACGACA AGCCAGTGTT AAGCATCTCG CCCAGGAGTT GGAAGCGAAA 
TGTATCCTGG AGCCGGGATC GCCACCGCGA CCAGGTGGTG TCTCTCCCCC CAAGACGACT 
GAGAGCAAAA CGGAGACATG CACCTCCCAG CTGCATGTGA GCACCCTGAC TGTGATCCTC 
AAGCCAAGTC CAGAGCGCAG AGCTCCACAG CCACGACCCC GCCGATGCCT GGATCGTCAG 
AGCGATGAAC ATGCCCGAGA GCCATCCCAG AAGCGAAACC TCGCCGCGAT TCGCACCATG 
ATGCCAACGG AGGCCTATGC GCCACCAAAG AAAATCCGAC GCACTCGACA GGAGCGCACC 
GGTGCGGATA GCCAAATGTT TTTTCAGAAC GAACACCTCG AAACCCTGTT CTACAGCTGG 
CTCAGCTCGG AGAACGGTGT CCAGTTCGAC ATTACGAGTG TGTCTGACGG TCAGCAGACC 
ATTGAGATAG CCACCGAGGA GGGTAATCTT CTGGAGCAAC CGCTACTTGA GCCGAGCACC 
GCTACTCTGG AGGATGTGAG CCAAAAGTCC GCCGTCGAGC GGCTTCTGGA GGAGGCCATC 
GCAAAATTAG AGCTAGATAA CGAGTCCAAG AAGGAGACAC AAGTCGAGGA AAAGAAGGAG 
ATGGATACAG CTCAGGATCC CATCGTTCAA GTCACTCCGC GACGCATTAA GCGCCAGGCG 
CCGCCAGTGC CAGCCCCACG ACCCAGTCTA AGTCAAGTTA CCTCCAGTGC ATCCAGCTGC 
AAGCAATCCG AGGCTGAGGA AAGTGAATCC GGGTTGTCTA CACTCCCCAA GATAACCAGC 
GACGAATCCC AGCCAGAGAC GCCCAAGGAT GACTTGCAGA CAGGTGAATT TGATTTTGCC 
AAGCCCCAGC GTCCGCCACG CAAGAAGAAA ATGCGCCGAA CTCTTACATG GAAAAAGGAG 
AACTCCATTG TCGAGGCCAC TGCAATAACC TCAACGGACT CCGATTCAGA TTACAAGCCT 
CCGCTATCAG GAGCGGCCAA GAAGAAGTCC GGTGCTATCT GTAACCCACT TCCAATGCCC 
GAGCCAAGTT TCATTGAGTT GGATAAGTCA CTGATGCGCC ACTTGAACTC GCCCCGGAAG 
ATCAAGTCGG CATACACCCT CACCGTGATG TCATCTCCCT CGCCGAATGC AGACAGCGAT 
CAGTCTCCTA GTCAGACACC AAGGCAATCC CTGGTACAGG CCATGCGGGA CAGTTTCGTG 
GATCAGGGCT TTGAGACATG CTCGAACGAT CCGATGGACA ACAGTCCGAT ACGCCGATCT 
TCGCTTGGAG CCACGGAATC GAAGCCATCC GGTTTTTCCA CGCCAGTTAA GGGACGCCAT 
GCTTCCAGTC CGGCGCAACA ACAACTGTTC AGTCCGATTC TCATCCAGGA GCGCCCTCGT 
CGCAGTTCCC TGGCCATGCA AGTGATCCGG GAGGATCATC CGCTCGATCT GGACGCCACC 
TCCAGCTCAC CCTCCACACC GTGCAGTGAG CGGGAGTTCT TCGCCAATGC CCCGACTGTT 
GAAATTGATA ACTCCCAGGA TGAGAGTCCG ACCGGCAAGG CGCACTCAAT GTTCTGGATC 
ACCTCTGGGG ACTTCACAGT CTCCCTGGAA ATCTTCAAGA ACAGCCCGGA GCGCCTGCGA 
CTGCTGTACG AAATCTTCAC CCAGAAAAGC TGGGAGACTC GTGACCTGGC CTTCGGAATC 
GACGGACACA AGTTCATTCG CGGAGCTCCC AGTTCCGACT CCGTCCGACA GTCGCTGCCC 
GAGCGTCCAC CCAGTGTGAA GGGCTGCTCT CACTATTGGT TCGCCAGCGG AGATCTCGCA 
GTGCCCTTCA GCGGAAAACT GATGTCCAGC GAGAAGATCG AGCGGCTGTT TGCCTTCCTC 
AGCGGAGAGC AGTCGGAGTT GCGCTTCGGC GTTGACCACA TTGAGTTCAG CAGCGTGCCT 
GAGTTCTGGC CCACCACCCA GAAGTATTCC ATCGAGAGCA GCTACAGCAT ACTGGTGGGT 
CTGCAGACAG GTGCCAGCAA TGGCCTGGAG GGTCGCAGCA AGTACTCCTG GCCCAACAGC 
AGCATCAGTG CCAACCAGGC GATCAAGACC AGCGACCTGG ATCAGACGGA GTTCGAGTCC 
GATAGCTTCG GCAACAACAG TGGTAGGCTG TCCTTTTCGC CTGATCTCTT CTCCCTGGAC 
TACGAGGCGG TTCCCCTGGA CGAGCTGTTT GCCAAGGCTC CTCCCTCTGC CGCCACACCC 
GCCATGTCCG TGCCGCAAAT GATGCAGACC CTCAAGCAGC AGCAGTCCAA ACTTCGCAGT 
GTGGAGCAGC GCATTCGTGG CTACGCCAAG CCCGCGAATC TGGCCGACTC TTCGCTGGAG 
CACTGCCGCA ACACGCCGCA ATATGTCCAC AAGCTGCGCT CCATCATCCG GGCCATTGAC 
AACATTGGAC GCGACGATGG CTTCCGCGGC TGCTCGATGG AGCAGCTCGA GAGCTTCATG 
TACTTCCTCA GCGAGTATGC GGATGTGTGC CTGGCCAACT GCAGCGAGCA CATGGACAAG 
ATTCTCGACA CCCTGATGGA CCGGAGGGCC GTGGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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slam cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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