Bsg25D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bsg25D
Entrez Gene ID	33757
Full Name	Blastoderm-specific gene 25D
Synonyms	CG14025,Dmel\CG14025,Nin,brg25D,bsg25D,bsq25D,nin
General protein information	Preferred Names Blastoderm-specific gene 25D Names CG14025 gene product from transcript CG14025-RF Bsg25D-PB Bsg25D-PC Bsg25D-PD Bsg25D-PE Bsg25D-PF Bsg25D-PG Bsg25D-PH CG14025-PB CG14025-PC CG14025-PD CG14025-PE CG14025-PF CG14025-PG CG14025-PH ninein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25D2-25D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_164637.3	NP_723072.1	Blastoderm-specific gene 25D, isoform B
NM_164638.3	NP_723073.1	Blastoderm-specific gene 25D, isoform C
NM_001201770.2	NP_001188699.1	Blastoderm-specific gene 25D, isoform E
NM_001273163.2	NP_001260092.1	Blastoderm-specific gene 25D, isoform F
NM_001273164.2	NP_001260093.1	Blastoderm-specific gene 25D, isoform G
NM_001201769.2	NP_001188698.1	Blastoderm-specific gene 25D, isoform D
NM_057568.6	NP_476916.4	Blastoderm-specific gene 25D, isoform H

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Bsg25D	NP_001260092.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007888	XP_317603.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bsg25D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bsg25D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13795	NM_001201769.2 Latest version!	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa21593	NM_057568.6 Latest version!	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13792	NM_001273163.2 Latest version!	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa13793	NM_001201770.2 Latest version!	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa13794	NM_001273164.2 Latest version!	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa16260	NM_164637.3 Latest version!	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa16261	NM_164638.3 Latest version!	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bsg25D ( NM_001201769.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188698.1 Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa13795
Clone ID Related Accession (Same CDS sequence)	NM_001201769.2
Accession Version	NM_001201769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blastoderm-specific gene 25D, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201769.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAACAC GATTGTATTA CCGCCGATCC GGCGGTCAGT TAACAGATCC GATAATTGAG 
AAGCTAGCCG CTCGTTTTGA GCGTTCACTG GTGATTACGG ATGAGCCGCT AAACAACACA 
TACATCGAGA GTCCGCCGGA GTCTTCCGAT CGCGAGGTTT CACCCAAACT CGTCGTGGGC 
ACCAAGAAAT ACGGTCGCCG GTCTAGGCCA CAGCAGGGAA TCTACGAGTT ATCCGTCACG 
GACTCGGACA ATACGGACGA GGACCAGTTG CAGCAGCAGC AAAATCAGCG AAGCCTCAAC 
GGATGCGATG AGCTGGGAGT TCAGGTGCAA CGTTCCTCGT CCCAGAGCGA TCTTCCTGGC 
AGCCGGCGTC TGCGGTCCGT CCACACCAGC GGGAGCAAAC TGAAGCGTTG TGCTTCACTG 
CCAGCACGCC GGAAGATGAA CAGCAACACC ACGGGAGCCA CTACATCACC GACGGCAGCG 
GCCAAGTTGA AACAGCTTTC CATCCAGAGC CAGGCGCAGC ACAGCAGCAG CGTGGAATCA 
CTGGACACCG TGACGCCGCA GCAATTGGAG ACGATCTCAG TGCATAGCAT TATGGAAGCC 
TGGGAGCTGG CCAGCATTCC CAACACTCGC AACCTACTTC ACGTCCTGGG ATTCGATGAG 
GAGGAGGAGG TGAACCTGCA GCAGCTAACT AAGGCATTGG AGGAGGAGCT GCGGGGCATC 
GATGGGGATC ACGAGCAATC GAATATGTTG CGCGCTCTGG CTGCTCTGCA GGCCACCGAG 
TTGGGCAACT ACAGACTTGC CTATAGGCAG CAGCATGAGG AGAACCTCAA GCTGAGGGCC 
GATAATAAGG CGGCCAACCA AAGGGTGGCT TTGCTTGCCG TGGAAGTGGA TGAGCGGCAT 
GCGTCGCTGG AGGATAACTC CAAGAAGCAG GTGCAGCAGC TGGAGCAAAG ACACGCCAGC 
ATGGTGCGTG AAATAACGCT GCGGATGACT AATGACCGCG ATCACTGGAC CAGCATGACG 
GGAAAGCTGG AGGCACAGCT TAAATCGCTT GAGCAGGAGG AGATCCGTCT GAGAACGGAA 
CTTGAACTGG TGCGCACTGA GAACACGGAG CTTGAGTCGG AGCAGCAAAA GGCTCACATC 
CAAATCACAG AGCTTCTCGA ACAGAACATT AAGCTCAACC AGGAACTGGC CCAAACGTCG 
AGCAGCATTG GTGGCACCCC GGAGCACAGT CCATTGCGAC CGAGAAGGCA TAGCGAGGAC 
AAGGAGGAGG AGATGCTCCA GCTAATGGAG AAGCTGGCTG CTCTTCAAAT GGAGAACGCC 
CAGCTGCGTG ACAAGACTGA CGAACTGACC ATCGAAATCG AGAGCTTAAA TGTGGAACTA 
ATTCGCTCGA AAACCAAGGC TAAAAAGCAA GAAAAACAGG AGAAACAAGA GGACCAGGAG 
TCGGCGGCCA CGGCTACCAA AAGGCGTGGG GATTCGCCGA GCAAAACACA TCTAACAGAG 
GAGAGCCCTC GCTTGGGGAA ACAGCGCAAG TGCACCGAAG GAGAGCAGAG CGATGCCAGC 
AACAGCGGAG ATTGGTTGGC TCTAAACTCC GAGCTGCAAA GAAGTCAAAG CCAGGATGAG 
GAGCTAACAA GCCTTAGACA GCGGGTTGCT GAGCTAGAGG AGGAACTCAA GGCTGCAAAG 
GAAGGCAGAT CTCTCACCCC GGAAAGCCGT TCGAAGGAAC TGGAGACCAG TCTAGAGCAA 
ATGCAGCGTG CCTATGAGGA TTGCGAGGAC TACTGGCAAA CGAAACTTAG CGAGGAGCGG 
CAGCTGTTTG AGAAGGAGCG ACAGATCTAC GAAGATGAGC AGCACGAGAG CGACAAGAAG 
TTCACCGAGC TGATGGAAAA GGTGCGCGAG TACGAGGAGC AGTTCAGCAA GGATGGCCGC 
CTCTCGCCCA TTGATGAGCG CGATATGCTG GAACAGCAGT ACTCGGAATT GGAGGCAGAG 
GCAGCCCAGC TGCGCTCGAG TTCCATTCAA ATGCTCGAGG AGAAGGCTCA GGAAATCAGT 
TCACTGCAAT CAGAGATCGA GGATTTGCGA CAGAGATTGG GTGAGAGCGT TGAGATCCTC 
ACAGGCGCCT GTGAACTCAC CTCGGAGTCG GTAGCCCAAC TGAGTGCCGA GGCGGGAAAA 
AGTCCAGCCA GCTCACCCAT CAGCTACCTC TGGCTGCAGA GCACCATCCA AGAGCCAGCG 
AAATCGCTTG CCGATTCCAA GGATGAAGCC ACCGCCAGTG CCATCGAATT GCTCGGAGGC 
TCACCATCGC ACAAGACAGC CAGCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188698.1
CDS	17..2326
Translation	MGTRLYYRRSGGQLTDPIIEKLAARFERSLVITDEPLNNTYIESPPESSDREVSPKLVVGTKKYGRRSRPQQGIYELSVTDSDNTDEDQLQQQQNQRSLNGCDELGVQVQRSSSQSDLPGSRRLRSVHTSGSKLKRCASLPARRKMNSNTTGATTSPTAAAKLKQLSIQSQAQHSSSVESLDTVTPQQLETISVHSIMEAWELASIPNTRNLLHVLGFDEEEEVNLQQLTKALEEELRGIDGDHEQSNMLRALAALQATELGNYRLAYRQQHEENLKLRADNKAANQRVALLAVEVDERHASLEDNSKKQVQQLEQRHASMVREITLRMTNDRDHWTSMTGKLEAQLKSLEQEEIRLRTELELVRTENTELESEQQKAHIQITELLEQNIKLNQELAQTSSSIGGTPEHSPLRPRRHSEDKEEEMLQLMEKLAALQMENAQLRDKTDELTIEIESLNVELIRSKTKAKKQEKQEKQEDQESAATATKRRGDSPSKTHLTEESPRLGKQRKCTEGEQSDASNSGDWLALNSELQRSQSQDEELTSLRQRVAELEEELKAAKEGRSLTPESRSKELETSLEQMQRAYEDCEDYWQTKLSEERQLFEKERQIYEDEQHESDKKFTELMEKVREYEEQFSKDGRLSPIDERDMLEQQYSELEAEAAQLRSSSIQMLEEKAQEISSLQSEIEDLRQRLGESVEILTGACELTSESVAQLSAEAGKSPASSPISYLWLQSTIQEPAKSLADSKDEATASAIELLGGSPSHKTASR

Target ORF information:

RefSeq Version	NM_001201769.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201769.2

ORF Insert Sequence:

ATGGGAACAC GATTGTATTA CCGCCGATCC GGCGGTCAGT TAACAGATCC GATAATTGAG 
AAGCTAGCCG CTCGTTTTGA GCGTTCACTG GTGATTACGG ATGAGCCGCT AAACAACACA 
TACATCGAGA GTCCGCCGGA GTCTTCCGAT CGCGAGGTTT CACCCAAACT CGTCGTGGGC 
ACCAAGAAAT ACGGTCGCCG GTCTAGGCCA CAGCAGGGAA TCTACGAGTT ATCCGTCACG 
GACTCGGACA ATACGGACGA GGACCAGTTG CAGCAGCAGC AAAATCAGCG AAGCCTCAAC 
GGATGCGATG AGCTGGGAGT TCAGGTGCAA CGTTCCTCGT CCCAGAGCGA TCTTCCTGGC 
AGCCGGCGTC TGCGGTCCGT CCACACCAGC GGGAGCAAAC TGAAGCGTTG TGCTTCACTG 
CCAGCACGCC GGAAGATGAA CAGCAACACC ACGGGAGCCA CTACATCACC GACGGCAGCG 
GCCAAGTTGA AACAGCTTTC CATCCAGAGC CAGGCGCAGC ACAGCAGCAG CGTGGAATCA 
CTGGACACCG TGACGCCGCA GCAATTGGAG ACGATCTCAG TGCATAGCAT TATGGAAGCC 
TGGGAGCTGG CCAGCATTCC CAACACTCGC AACCTACTTC ACGTCCTGGG ATTCGATGAG 
GAGGAGGAGG TGAACCTGCA GCAGCTAACT AAGGCATTGG AGGAGGAGCT GCGGGGCATC 
GATGGGGATC ACGAGCAATC GAATATGTTG CGCGCTCTGG CTGCTCTGCA GGCCACCGAG 
TTGGGCAACT ACAGACTTGC CTATAGGCAG CAGCATGAGG AGAACCTCAA GCTGAGGGCC 
GATAATAAGG CGGCCAACCA AAGGGTGGCT TTGCTTGCCG TGGAAGTGGA TGAGCGGCAT 
GCGTCGCTGG AGGATAACTC CAAGAAGCAG GTGCAGCAGC TGGAGCAAAG ACACGCCAGC 
ATGGTGCGTG AAATAACGCT GCGGATGACT AATGACCGCG ATCACTGGAC CAGCATGACG 
GGAAAGCTGG AGGCACAGCT TAAATCGCTT GAGCAGGAGG AGATCCGTCT GAGAACGGAA 
CTTGAACTGG TGCGCACTGA GAACACGGAG CTTGAGTCGG AGCAGCAAAA GGCTCACATC 
CAAATCACAG AGCTTCTCGA ACAGAACATT AAGCTCAACC AGGAACTGGC CCAAACGTCG 
AGCAGCATTG GTGGCACCCC GGAGCACAGT CCATTGCGAC CGAGAAGGCA TAGCGAGGAC 
AAGGAGGAGG AGATGCTCCA GCTAATGGAG AAGCTGGCTG CTCTTCAAAT GGAGAACGCC 
CAGCTGCGTG ACAAGACTGA CGAACTGACC ATCGAAATCG AGAGCTTAAA TGTGGAACTA 
ATTCGCTCGA AAACCAAGGC TAAAAAGCAA GAAAAACAGG AGAAACAAGA GGACCAGGAG 
TCGGCGGCCA CGGCTACCAA AAGGCGTGGG GATTCGCCGA GCAAAACACA TCTAACAGAG 
GAGAGCCCTC GCTTGGGGAA ACAGCGCAAG TGCACCGAAG GAGAGCAGAG CGATGCCAGC 
AACAGCGGAG ATTGGTTGGC TCTAAACTCC GAGCTGCAAA GAAGTCAAAG CCAGGATGAG 
GAGCTAACAA GCCTTAGACA GCGGGTTGCT GAGCTAGAGG AGGAACTCAA GGCTGCAAAG 
GAAGGCAGAT CTCTCACCCC GGAAAGCCGT TCGAAGGAAC TGGAGACCAG TCTAGAGCAA 
ATGCAGCGTG CCTATGAGGA TTGCGAGGAC TACTGGCAAA CGAAACTTAG CGAGGAGCGG 
CAGCTGTTTG AGAAGGAGCG ACAGATCTAC GAAGATGAGC AGCACGAGAG CGACAAGAAG 
TTCACCGAGC TGATGGAAAA GGTGCGCGAG TACGAGGAGC AGTTCAGCAA GGATGGCCGC 
CTCTCGCCCA TTGATGAGCG CGATATGCTG GAACAGCAGT ACTCGGAATT GGAGGCAGAG 
GCAGCCCAGC TGCGCTCGAG TTCCATTCAA ATGCTCGAGG AGAAGGCTCA GGAAATCAGT 
TCACTGCAAT CAGAGATCGA GGATTTGCGA CAGAGATTGG GTGAGAGCGT TGAGATCCTC 
ACAGGCGCCT GTGAACTCAC CTCGGAGTCG GTAGCCCAAC TGAGTGCCGA GGCGGGAAAA 
AGTCCAGCCA GCTCACCCAT CAGCTACCTC TGGCTGCAGA GCACCATCCA AGAGCCAGCG 
AAATCGCTTG CCGATTCCAA GGATGAAGCC ACCGCCAGTG CCATCGAATT GCTCGGAGGC 
TCACCATCGC ACAAGACAGC CAGCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bsg25D ( NM_057568.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476916.4 Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa21593
Clone ID Related Accession (Same CDS sequence)	NM_057568.6
Accession Version	NM_057568.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blastoderm-specific gene 25D, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057568.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGAGCGTTCA 
CTGGTGATTA CGGATGAGCC GCTAAACAAC ACATACATCG AGAGTCCGCC GGAGTCTTCC 
GATCGCGAGG TTTCACCCAA ACTCGTCGTG GGCACCAAGA AATACGGTCG CCGGTCTAGG 
CCACAGCAGG GAATCTACGA GTTATCCGTC ACGGACTCGG ACAATACGGA CGAGGACCAG 
TTGCAGCAGC AGCAAAATCA GCGAAGCCTC AACGGATGCG ATGAGCTGGG AGTTCAGGTG 
CAACGTTCCT CGTCCCAGAG CGATCTTCCT GGCAGCCGGC GTCTGCGGTC CGTCCACACC 
AGCGGGAGCA AACTGAAGCG TTGTGCTTCA CTGCCAGCAC GCCGGAAGAT GAACAGCAAC 
ACCACGGGAG CCACTACATC ACCGACGGCA GCGGCCAAGT TGAAACAGCT TTCCATCCAG 
AGCCAGGCGC AGCACAGCAG CAGCGTGGAA TCACTGGACA CCGTGACGCC GCAGCAATTG 
GAGACGATCT CAGTGCATAG CATTATGGAA GCCTGGGAGC TGGCCAGCAT TCCCAACACT 
CGCAACCTAC TTCACGTCCT GGGATTCGAT GAGGAGGAGG AGGTGAACCT GCAGCAGCTA 
ACTAAGGCAT TGGAGGAGGA GCTGCGGGGC ATCGATGGGG ATCACGAGCA ATCGAATATG 
TTGCGCGCTC TGGCTGCTCT GCAGGCCACC GAGTTGGGCA ACTACAGACT TGCCTATAGG 
CAGCAGCATG AGGAGAACCT CAAGCTGAGG GCCGATAATA AGGCGGCCAA CCAAAGGGTG 
GCTTTGCTTG CCGTGGAAGT GGATGAGCGG CATGCGTCGC TGGAGGATAA CTCCAAGAAG 
CAGGTGCAGC AGCTGGAGCA AAGACACGCC AGCATGGTGC GTGAAATAAC GCTGCGGATG 
ACTAATGACC GCGATCACTG GACCAGCATG ACGGGAAAGC TGGAGGCACA GCTTAAATCG 
CTTGAGCAGG AGGAGATCCG TCTGAGAACG GAACTTGAAC TGGTGCGCAC TGAGAACACG 
GAGCTTGAGT CGGAGCAGCA AAAGGCTCAC ATCCAAATCA CAGAGCTTCT CGAACAGAAC 
ATTAAGCTCA ACCAGGAACT GGCCCAAACG TCGAGCAGCA TTGGTGGCAC CCCGGAGCAC 
AGTCCATTGC GACCGAGAAG GCATAGCGAG GACAAGGAGG AGGAGATGCT CCAGCTAATG 
GAGAAGCTGG CTGCTCTTCA AATGGAGAAC GCCCAGCTGC GTGACAAGAC TGACGAACTG 
ACCATCGAAA TCGAGAGCTT AAATGTGGAA CTAATTCGCT CGAAAACCAA GGCTAAAAAG 
CAAGAAAAAC AGGAGAAACA AGAGGACCAG GAGTCGGCGG CCACGGCTAC CAAAAGGCGT 
GGGGATTCGC CGAGCAAAAC ACATCTAACA GAGGAGAGCC CTCGCTTGGG GAAACAGCGC 
AAGTGCACCG AAGGAGAGCA GAGCGATGCC AGCAACAGCG GAGATTGGTT GGCTCTAAAC 
TCCGAGCTGC AAAGAAGTCA AAGCCAGGAT GAGGAGCTAA CAAGCCTTAG ACAGCGGGTT 
GCTGAGCTAG AGGAGGAACT CAAGGCTGCA AAGGAAGGCA GATCTCTCAC CCCGGAAAGC 
CGTTCGAAGG AACTGGAGAC CAGTCTAGAG CAAATGCAGC GTGCCTATGA GGATTGCGAG 
GACTACTGGC AAACGAAACT TAGCGAGGAG CGGCAGCTGT TTGAGAAGGA GCGACAGATC 
TACGAAGATG AGCAGCACGA GAGCGACAAG AAGTTCACCG AGCTGATGGA AAAGGTGCGC 
GAGTACGAGG AGCAGTTCAG CAAGGATGGC CGCCTCTCGC CCATTGATGA GCGCGATATG 
CTGGAACAGC AGTACTCGGA ATTGGAGGCA GAGGCAGCCC AGCTGCGCTC GAGTTCCATT 
CAAATGCTCG AGGAGAAGGC TCAGGAAATC AGTTCACTGC AATCAGAGAT CGAGGATTTG 
CGACAGAGAT TGGGTGAGAG CGTTGAGATC CTCACAGGCG CCTGTGAACT CACCTCGGAG 
TCGGTAGCCC AACTGAGTGC CGAGGCGGGA AAAAGTCCAG CCAGCTCACC CATCAGCTAC 
CTCTGGCTGC AGAGCACCAT CCAAGAGCCA GCGAAATCGC TTGCCGATTC CAAGGATGAA 
GCCACCGCCA GTGCCATCGA ATTGCTCGGA GGCTCACCAT CGCACAAGAC AGCCAGCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476916.4
CDS	360..2822
Translation	MEVSADPYEQKLYQMFRSCETQCGLLDEKSLLKLCSLLELRDQGSALIASLGGSHQLGVSFGQFKEALLNFLGSEFDERSLVITDEPLNNTYIESPPESSDREVSPKLVVGTKKYGRRSRPQQGIYELSVTDSDNTDEDQLQQQQNQRSLNGCDELGVQVQRSSSQSDLPGSRRLRSVHTSGSKLKRCASLPARRKMNSNTTGATTSPTAAAKLKQLSIQSQAQHSSSVESLDTVTPQQLETISVHSIMEAWELASIPNTRNLLHVLGFDEEEEVNLQQLTKALEEELRGIDGDHEQSNMLRALAALQATELGNYRLAYRQQHEENLKLRADNKAANQRVALLAVEVDERHASLEDNSKKQVQQLEQRHASMVREITLRMTNDRDHWTSMTGKLEAQLKSLEQEEIRLRTELELVRTENTELESEQQKAHIQITELLEQNIKLNQELAQTSSSIGGTPEHSPLRPRRHSEDKEEEMLQLMEKLAALQMENAQLRDKTDELTIEIESLNVELIRSKTKAKKQEKQEKQEDQESAATATKRRGDSPSKTHLTEESPRLGKQRKCTEGEQSDASNSGDWLALNSELQRSQSQDEELTSLRQRVAELEEELKAAKEGRSLTPESRSKELETSLEQMQRAYEDCEDYWQTKLSEERQLFEKERQIYEDEQHESDKKFTELMEKVREYEEQFSKDGRLSPIDERDMLEQQYSELEAEAAQLRSSSIQMLEEKAQEISSLQSEIEDLRQRLGESVEILTGACELTSESVAQLSAEAGKSPASSPISYLWLQSTIQEPAKSLADSKDEATASAIELLGGSPSHKTASR

Target ORF information:

RefSeq Version	NM_057568.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057568.6

ORF Insert Sequence:

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGAGCGTTCA 
CTGGTGATTA CGGATGAGCC GCTAAACAAC ACATACATCG AGAGTCCGCC GGAGTCTTCC 
GATCGCGAGG TTTCACCCAA ACTCGTCGTG GGCACCAAGA AATACGGTCG CCGGTCTAGG 
CCACAGCAGG GAATCTACGA GTTATCCGTC ACGGACTCGG ACAATACGGA CGAGGACCAG 
TTGCAGCAGC AGCAAAATCA GCGAAGCCTC AACGGATGCG ATGAGCTGGG AGTTCAGGTG 
CAACGTTCCT CGTCCCAGAG CGATCTTCCT GGCAGCCGGC GTCTGCGGTC CGTCCACACC 
AGCGGGAGCA AACTGAAGCG TTGTGCTTCA CTGCCAGCAC GCCGGAAGAT GAACAGCAAC 
ACCACGGGAG CCACTACATC ACCGACGGCA GCGGCCAAGT TGAAACAGCT TTCCATCCAG 
AGCCAGGCGC AGCACAGCAG CAGCGTGGAA TCACTGGACA CCGTGACGCC GCAGCAATTG 
GAGACGATCT CAGTGCATAG CATTATGGAA GCCTGGGAGC TGGCCAGCAT TCCCAACACT 
CGCAACCTAC TTCACGTCCT GGGATTCGAT GAGGAGGAGG AGGTGAACCT GCAGCAGCTA 
ACTAAGGCAT TGGAGGAGGA GCTGCGGGGC ATCGATGGGG ATCACGAGCA ATCGAATATG 
TTGCGCGCTC TGGCTGCTCT GCAGGCCACC GAGTTGGGCA ACTACAGACT TGCCTATAGG 
CAGCAGCATG AGGAGAACCT CAAGCTGAGG GCCGATAATA AGGCGGCCAA CCAAAGGGTG 
GCTTTGCTTG CCGTGGAAGT GGATGAGCGG CATGCGTCGC TGGAGGATAA CTCCAAGAAG 
CAGGTGCAGC AGCTGGAGCA AAGACACGCC AGCATGGTGC GTGAAATAAC GCTGCGGATG 
ACTAATGACC GCGATCACTG GACCAGCATG ACGGGAAAGC TGGAGGCACA GCTTAAATCG 
CTTGAGCAGG AGGAGATCCG TCTGAGAACG GAACTTGAAC TGGTGCGCAC TGAGAACACG 
GAGCTTGAGT CGGAGCAGCA AAAGGCTCAC ATCCAAATCA CAGAGCTTCT CGAACAGAAC 
ATTAAGCTCA ACCAGGAACT GGCCCAAACG TCGAGCAGCA TTGGTGGCAC CCCGGAGCAC 
AGTCCATTGC GACCGAGAAG GCATAGCGAG GACAAGGAGG AGGAGATGCT CCAGCTAATG 
GAGAAGCTGG CTGCTCTTCA AATGGAGAAC GCCCAGCTGC GTGACAAGAC TGACGAACTG 
ACCATCGAAA TCGAGAGCTT AAATGTGGAA CTAATTCGCT CGAAAACCAA GGCTAAAAAG 
CAAGAAAAAC AGGAGAAACA AGAGGACCAG GAGTCGGCGG CCACGGCTAC CAAAAGGCGT 
GGGGATTCGC CGAGCAAAAC ACATCTAACA GAGGAGAGCC CTCGCTTGGG GAAACAGCGC 
AAGTGCACCG AAGGAGAGCA GAGCGATGCC AGCAACAGCG GAGATTGGTT GGCTCTAAAC 
TCCGAGCTGC AAAGAAGTCA AAGCCAGGAT GAGGAGCTAA CAAGCCTTAG ACAGCGGGTT 
GCTGAGCTAG AGGAGGAACT CAAGGCTGCA AAGGAAGGCA GATCTCTCAC CCCGGAAAGC 
CGTTCGAAGG AACTGGAGAC CAGTCTAGAG CAAATGCAGC GTGCCTATGA GGATTGCGAG 
GACTACTGGC AAACGAAACT TAGCGAGGAG CGGCAGCTGT TTGAGAAGGA GCGACAGATC 
TACGAAGATG AGCAGCACGA GAGCGACAAG AAGTTCACCG AGCTGATGGA AAAGGTGCGC 
GAGTACGAGG AGCAGTTCAG CAAGGATGGC CGCCTCTCGC CCATTGATGA GCGCGATATG 
CTGGAACAGC AGTACTCGGA ATTGGAGGCA GAGGCAGCCC AGCTGCGCTC GAGTTCCATT 
CAAATGCTCG AGGAGAAGGC TCAGGAAATC AGTTCACTGC AATCAGAGAT CGAGGATTTG 
CGACAGAGAT TGGGTGAGAG CGTTGAGATC CTCACAGGCG CCTGTGAACT CACCTCGGAG 
TCGGTAGCCC AACTGAGTGC CGAGGCGGGA AAAAGTCCAG CCAGCTCACC CATCAGCTAC 
CTCTGGCTGC AGAGCACCAT CCAAGAGCCA GCGAAATCGC TTGCCGATTC CAAGGATGAA 
GCCACCGCCA GTGCCATCGA ATTGCTCGGA GGCTCACCAT CGCACAAGAC AGCCAGCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bsg25D ( NM_001273163.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260092.1 Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa13792
Clone ID Related Accession (Same CDS sequence)	NM_001273163.2
Accession Version	NM_001273163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blastoderm-specific gene 25D, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273163.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGAGCGTTCA 
CTGGTGATTA CGGATGAGCC GCTAAACAAC ACATACATCG AGAGTCCGCC GGAGTCTTCC 
GATCGCGAGG TTTCACCCAA ACTCGTCGTG GGCACCAAGA AATACGGTCG CCGGTCTAGG 
CCACAGCAGG GAATCTACGA GTTATCCGTC ACGGACTCGG ACAATACGGA CGAGGACCAG 
TTGCAGCAGC AGCAAAATCA GCGAAGCCTC AACGGATGCG ATGAGCTGGG AGTTCAGGTG 
CAACGTTCCT CGTCCCAGAG CGATCTTCCT GGCAGCCGGC GTCTGCGGTC CGTCCACACC 
AGCGGGAGCA AACTGAAGCG TTGTGCTTCA CTGCCAGCAC GCCGGAAGAT GAACAGCAAC 
ACCACGGGAG CCACTACATC ACCGACGGCA GCGGCCAAGT TGAAACAGCT TTCCATCCAG 
AGCCAGGCGC AGCACAGCAG CAGCGTGGAA TCACTGGACA CCGTGACGCC GCAGCAATTG 
GAGACGATCT CAGTGCATAG CATTATGGAA GCCTGGGAGC TGGCCAGCAT TCCCAACACT 
CGCAACCTAC TTCACGTCCT GGGATTCGAT GAGGAGGAGG AGGTGAACCT GCAGCAGCTA 
ACTAAGGCAT TGGAGGAGGA GCTGCGGGGC ATCGATGGGG ATCACGAGCA ATCGAATATG 
TTGCGCGCTC TGGCTGCTCT GCAGGCCACC GAGTTGGGCA ACTACAGACT TGCCTATAGG 
CAGCAGCATG AGGAGAACCT CAAGCTGAGG GCCGATAATA AGGCGGCCAA CCAAAGGGTG 
GCTTTGCTTG CCGTGGAAGT GGATGAGCGG CATGCGTCGC TGGAGGATAA CTCCAAGAAG 
CAGGTGCAGC AGCTGGAGCA AAGACACGCC AGCATGGTGC GTGAAATAAC GCTGCGGATG 
ACTAATGACC GCGATCACTG GACCAGCATG ACGGGAAAGC TGGAGGCACA GCTTAAATCG 
CTTGAGCAGG AGGAGATCCG TCTGAGAACG GAACTTGAAC TGGTGCGCAC TGAGAACACG 
GAGCTTGAGT CGGAGCAGCA AAAGGCTCAC ATCCAAATCA CAGAGCTTCT CGAACAGAAC 
ATTAAGCTCA ACCAGGAACT GGCCCAAACG TCGAGCAGCA TTGGTGGCAC CCCGGAGCAC 
AGTCCATTGC GACCGAGAAG GCATAGCGAG GACAAGGAGG AGGAGATGCT CCAGCTAATG 
GAGAAGCTGG CTGCTCTTCA AATGGAGAAC GCCCAGCTGC GTGACAAGAC TGACGAACTG 
ACCATCGAAA TCGAGAGCTT AAATGTGGAA CTAATTCGCT CGAAAACCAA GGCTAAAAAG 
CAAGAAAAAC AGGAGAAACA AGAGGACCAG GAGTCGGCGG CCACGGCTAC CAAAAGGCGT 
GGGGATTCGC CGAGCAAAAC ACATCTAACA GAGGAGAGCC CTCGCTTGGG GAAACAGCGC 
AAGTGCACCG AAGGAGAGCA GAGCGATGCC AGCAACAGCG GAGATTGGTT GGCTCTAAAC 
TCCGAGCTGC AAAGAAGTCA AAGCCAGGAT GAGGAGCTAA CAAGCCTTAG ACAGCGGGTT 
GCTGAGCTAG AGGAGGAACT CAAGGCTGCA AAGGAAGGCA GATCTCTCAC CCCGGAAAGC 
CGTTCGAAGG AACTGGAGAC CAGTCTAGAG CAAATGCAGC GTGCCTATGA GGATTGCGAG 
GACTACTGGC AAACGAAACT TAGCGAGGAG CGGCAGCTGT TTGAGAAGGA GCGACAGATC 
TACGAAGATG AGCAGCACGA GAGCGACAAG AAGTTCACCG AGCTGATGGA AAAGGTGCGC 
GAGTACGAGG AGCAGTTCAG CAAGGATGGC CGCCTCTCGC CCATTGATGA GCGCGATATG 
CTGGAACAGC AGTACTCGGA ATTGGAGGCA GAGGCAGCCC AGCTGCGCTC GAGTTCCATT 
CAAATGCTCG AGGAGAAGGC TCAGGAAATC AGTTCACTGC AATCAGAGAT CGAGGATTTG 
CGACAGAGAT TGGGTGAGAG CGTTGAGATC CTCACAGGCG CCTGTGAACT CACCTCGGAG 
TCGGTAGCCC AACTGAGTGC CGAGGCGGGA AAAAGTCCAG CCAGCTCACC CATCAGCTAC 
CTCTGGCTGC AGAGCACCAT CCAAGAGCCA GCGAAATCGC TTGCCGATTC CAAGGATGAA 
GCCACCGCCA GTGCCATCGA ATTGCTCGGA GGCTCACCAT CGCACAAGAC AGCCAGCCGC 
ACTACACCCT TCGAAAGCTC TCAGTCGGGT CCTTCGCCCA CGAACAGTGG CAACAGCAAC 
GCCTACGGCG CCAATCCCGG CCCAGCTCCG ATCAGCAAGC CCAAGCGGTC CCAGAGTCCC 
CAACAGGCGG CTGCATCGGA GGGAGAGATA GCCGATTGCG AGACGTCGTC GACGGCGTCC 
GGCAAAAGCT TCGAATCCAA CAGTAAAACG TCTTGCCTTA GCCACGAGAA GTGCAGCAGT 
CCGTCGGCAC TGAAGGAGGA ACTGAAGCGC CTTAAGTTCT TCGAGCTCTC CCTCAAGGAG 
CAAATCAAGG ATCTGAGTCT GCAGCGGGAC GGTCTGGTCA TGGAACTGCA GCAGTTGCAG 
GAGGCGCGAC CCGTGCTCGA GAAGGCCTAT GCGCGAACAA CGCATCCAAC GCTTCAGCAG 
CGACTGAACC AATTGGAGCT GCGAAATCGC CATCTGCAGA ATGTCATCAA GCAGCAGCAG 
CATTACACGG AGTCCCTGAT GCAGCAATCC TGGCGGCAGC ATCAAGTGGA GCTCAACGAT 
TTGCATAGCC GAATCGAGAC CCAGGGTGTT TTACTGGCCG ATCAGACACA GCGATTGCAG 
AGTGCCGACA TCCTGGTGAA GGATCTATAT GTGGAGAACT CCCATCTGAC GGCTACGGTG 
CAGCGGTTGG AGCAGCAACG AGCTAGGGTG AACCTCATTC ACCAGCAGCA GCAACAGCAG 
CGCCTTGTGG GCGGTGGACT GCCTGGCATG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260092.1
CDS	360..3575
Translation	MEVSADPYEQKLYQMFRSCETQCGLLDEKSLLKLCSLLELRDQGSALIASLGGSHQLGVSFGQFKEALLNFLGSEFDERSLVITDEPLNNTYIESPPESSDREVSPKLVVGTKKYGRRSRPQQGIYELSVTDSDNTDEDQLQQQQNQRSLNGCDELGVQVQRSSSQSDLPGSRRLRSVHTSGSKLKRCASLPARRKMNSNTTGATTSPTAAAKLKQLSIQSQAQHSSSVESLDTVTPQQLETISVHSIMEAWELASIPNTRNLLHVLGFDEEEEVNLQQLTKALEEELRGIDGDHEQSNMLRALAALQATELGNYRLAYRQQHEENLKLRADNKAANQRVALLAVEVDERHASLEDNSKKQVQQLEQRHASMVREITLRMTNDRDHWTSMTGKLEAQLKSLEQEEIRLRTELELVRTENTELESEQQKAHIQITELLEQNIKLNQELAQTSSSIGGTPEHSPLRPRRHSEDKEEEMLQLMEKLAALQMENAQLRDKTDELTIEIESLNVELIRSKTKAKKQEKQEKQEDQESAATATKRRGDSPSKTHLTEESPRLGKQRKCTEGEQSDASNSGDWLALNSELQRSQSQDEELTSLRQRVAELEEELKAAKEGRSLTPESRSKELETSLEQMQRAYEDCEDYWQTKLSEERQLFEKERQIYEDEQHESDKKFTELMEKVREYEEQFSKDGRLSPIDERDMLEQQYSELEAEAAQLRSSSIQMLEEKAQEISSLQSEIEDLRQRLGESVEILTGACELTSESVAQLSAEAGKSPASSPISYLWLQSTIQEPAKSLADSKDEATASAIELLGGSPSHKTASRTTPFESSQSGPSPTNSGNSNAYGANPGPAPISKPKRSQSPQQAAASEGEIADCETSSTASGKSFESNSKTSCLSHEKCSSPSALKEELKRLKFFELSLKEQIKDLSLQRDGLVMELQQLQEARPVLEKAYARTTHPTLQQRLNQLELRNRHLQNVIKQQQHYTESLMQQSWRQHQVELNDLHSRIETQGVLLADQTQRLQSADILVKDLYVENSHLTATVQRLEQQRARVNLIHQQQQQQRLVGGGLPGMP

Target ORF information:

RefSeq Version	NM_001273163.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273163.2

ORF Insert Sequence:

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGAGCGTTCA 
CTGGTGATTA CGGATGAGCC GCTAAACAAC ACATACATCG AGAGTCCGCC GGAGTCTTCC 
GATCGCGAGG TTTCACCCAA ACTCGTCGTG GGCACCAAGA AATACGGTCG CCGGTCTAGG 
CCACAGCAGG GAATCTACGA GTTATCCGTC ACGGACTCGG ACAATACGGA CGAGGACCAG 
TTGCAGCAGC AGCAAAATCA GCGAAGCCTC AACGGATGCG ATGAGCTGGG AGTTCAGGTG 
CAACGTTCCT CGTCCCAGAG CGATCTTCCT GGCAGCCGGC GTCTGCGGTC CGTCCACACC 
AGCGGGAGCA AACTGAAGCG TTGTGCTTCA CTGCCAGCAC GCCGGAAGAT GAACAGCAAC 
ACCACGGGAG CCACTACATC ACCGACGGCA GCGGCCAAGT TGAAACAGCT TTCCATCCAG 
AGCCAGGCGC AGCACAGCAG CAGCGTGGAA TCACTGGACA CCGTGACGCC GCAGCAATTG 
GAGACGATCT CAGTGCATAG CATTATGGAA GCCTGGGAGC TGGCCAGCAT TCCCAACACT 
CGCAACCTAC TTCACGTCCT GGGATTCGAT GAGGAGGAGG AGGTGAACCT GCAGCAGCTA 
ACTAAGGCAT TGGAGGAGGA GCTGCGGGGC ATCGATGGGG ATCACGAGCA ATCGAATATG 
TTGCGCGCTC TGGCTGCTCT GCAGGCCACC GAGTTGGGCA ACTACAGACT TGCCTATAGG 
CAGCAGCATG AGGAGAACCT CAAGCTGAGG GCCGATAATA AGGCGGCCAA CCAAAGGGTG 
GCTTTGCTTG CCGTGGAAGT GGATGAGCGG CATGCGTCGC TGGAGGATAA CTCCAAGAAG 
CAGGTGCAGC AGCTGGAGCA AAGACACGCC AGCATGGTGC GTGAAATAAC GCTGCGGATG 
ACTAATGACC GCGATCACTG GACCAGCATG ACGGGAAAGC TGGAGGCACA GCTTAAATCG 
CTTGAGCAGG AGGAGATCCG TCTGAGAACG GAACTTGAAC TGGTGCGCAC TGAGAACACG 
GAGCTTGAGT CGGAGCAGCA AAAGGCTCAC ATCCAAATCA CAGAGCTTCT CGAACAGAAC 
ATTAAGCTCA ACCAGGAACT GGCCCAAACG TCGAGCAGCA TTGGTGGCAC CCCGGAGCAC 
AGTCCATTGC GACCGAGAAG GCATAGCGAG GACAAGGAGG AGGAGATGCT CCAGCTAATG 
GAGAAGCTGG CTGCTCTTCA AATGGAGAAC GCCCAGCTGC GTGACAAGAC TGACGAACTG 
ACCATCGAAA TCGAGAGCTT AAATGTGGAA CTAATTCGCT CGAAAACCAA GGCTAAAAAG 
CAAGAAAAAC AGGAGAAACA AGAGGACCAG GAGTCGGCGG CCACGGCTAC CAAAAGGCGT 
GGGGATTCGC CGAGCAAAAC ACATCTAACA GAGGAGAGCC CTCGCTTGGG GAAACAGCGC 
AAGTGCACCG AAGGAGAGCA GAGCGATGCC AGCAACAGCG GAGATTGGTT GGCTCTAAAC 
TCCGAGCTGC AAAGAAGTCA AAGCCAGGAT GAGGAGCTAA CAAGCCTTAG ACAGCGGGTT 
GCTGAGCTAG AGGAGGAACT CAAGGCTGCA AAGGAAGGCA GATCTCTCAC CCCGGAAAGC 
CGTTCGAAGG AACTGGAGAC CAGTCTAGAG CAAATGCAGC GTGCCTATGA GGATTGCGAG 
GACTACTGGC AAACGAAACT TAGCGAGGAG CGGCAGCTGT TTGAGAAGGA GCGACAGATC 
TACGAAGATG AGCAGCACGA GAGCGACAAG AAGTTCACCG AGCTGATGGA AAAGGTGCGC 
GAGTACGAGG AGCAGTTCAG CAAGGATGGC CGCCTCTCGC CCATTGATGA GCGCGATATG 
CTGGAACAGC AGTACTCGGA ATTGGAGGCA GAGGCAGCCC AGCTGCGCTC GAGTTCCATT 
CAAATGCTCG AGGAGAAGGC TCAGGAAATC AGTTCACTGC AATCAGAGAT CGAGGATTTG 
CGACAGAGAT TGGGTGAGAG CGTTGAGATC CTCACAGGCG CCTGTGAACT CACCTCGGAG 
TCGGTAGCCC AACTGAGTGC CGAGGCGGGA AAAAGTCCAG CCAGCTCACC CATCAGCTAC 
CTCTGGCTGC AGAGCACCAT CCAAGAGCCA GCGAAATCGC TTGCCGATTC CAAGGATGAA 
GCCACCGCCA GTGCCATCGA ATTGCTCGGA GGCTCACCAT CGCACAAGAC AGCCAGCCGC 
ACTACACCCT TCGAAAGCTC TCAGTCGGGT CCTTCGCCCA CGAACAGTGG CAACAGCAAC 
GCCTACGGCG CCAATCCCGG CCCAGCTCCG ATCAGCAAGC CCAAGCGGTC CCAGAGTCCC 
CAACAGGCGG CTGCATCGGA GGGAGAGATA GCCGATTGCG AGACGTCGTC GACGGCGTCC 
GGCAAAAGCT TCGAATCCAA CAGTAAAACG TCTTGCCTTA GCCACGAGAA GTGCAGCAGT 
CCGTCGGCAC TGAAGGAGGA ACTGAAGCGC CTTAAGTTCT TCGAGCTCTC CCTCAAGGAG 
CAAATCAAGG ATCTGAGTCT GCAGCGGGAC GGTCTGGTCA TGGAACTGCA GCAGTTGCAG 
GAGGCGCGAC CCGTGCTCGA GAAGGCCTAT GCGCGAACAA CGCATCCAAC GCTTCAGCAG 
CGACTGAACC AATTGGAGCT GCGAAATCGC CATCTGCAGA ATGTCATCAA GCAGCAGCAG 
CATTACACGG AGTCCCTGAT GCAGCAATCC TGGCGGCAGC ATCAAGTGGA GCTCAACGAT 
TTGCATAGCC GAATCGAGAC CCAGGGTGTT TTACTGGCCG ATCAGACACA GCGATTGCAG 
AGTGCCGACA TCCTGGTGAA GGATCTATAT GTGGAGAACT CCCATCTGAC GGCTACGGTG 
CAGCGGTTGG AGCAGCAACG AGCTAGGGTG AACCTCATTC ACCAGCAGCA GCAACAGCAG 
CGCCTTGTGG GCGGTGGACT GCCTGGCATG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bsg25D ( NM_001201770.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188699.1 Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa13793
Clone ID Related Accession (Same CDS sequence)	NM_001201770.2
Accession Version	NM_001201770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blastoderm-specific gene 25D, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201770.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGGTAATACG 
TCATCGGGTT TCATTGAGCG TTCACTGGTG ATTACGGATG AGCCGCTAAA CAACACATAC 
ATCGAGAGTC CGCCGGAGTC TTCCGATCGC GAGGTTTCAC CCAAACTCGT CGTGGGCACC 
AAGAAATACG GTCGCCGGTC TAGGCCACAG CAGGGAATCT ACGAGTTATC CGTCACGGAC 
TCGGACAATA CGGACGAGGA CCAGTTGCAG CAGCAGCAAA ATCAGCGAAG CCTCAACGGA 
TGCGATGAGC TGGGAGTTCA GGTGCAACGT TCCTCGTCCC AGAGCGATCT TCCTGGCAGC 
CGGCGTCTGC GGTCCGTCCA CACCAGCGGG AGCAAACTGA AGCGTTGTGC TTCACTGCCA 
GCACGCCGGA AGATGAACAG CAACACCACG GGAGCCACTA CATCACCGAC GGCAGCGGCC 
AAGTTGAAAC AGCTTTCCAT CCAGAGCCAG GCGCAGCACA GCAGCAGCGT GGAATCACTG 
GACACCGTGA CGCCGCAGCA ATTGGAGACG ATCTCAGTGC ATAGCATTAT GGAAGCCTGG 
GAGCTGGCCA GCATTCCCAA CACTCGCAAC CTACTTCACG TCCTGGGATT CGATGAGGAG 
GAGGAGGTGA ACCTGCAGCA GCTAACTAAG GCATTGGAGG AGGAGCTGCG GGGCATCGAT 
GGGGATCACG AGCAATCGAA TATGTTGCGC GCTCTGGCTG CTCTGCAGGC CACCGAGTTG 
GGCAACTACA GACTTGCCTA TAGGCAGCAG CATGAGGAGA ACCTCAAGCT GAGGGCCGAT 
AATAAGGCGG CCAACCAAAG GGTGGCTTTG CTTGCCGTGG AAGTGGATGA GCGGCATGCG 
TCGCTGGAGG ATAACTCCAA GAAGCAGGTG CAGCAGCTGG AGCAAAGACA CGCCAGCATG 
GTGCGTGAAA TAACGCTGCG GATGACTAAT GACCGCGATC ACTGGACCAG CATGACGGGA 
AAGCTGGAGG CACAGCTTAA ATCGCTTGAG CAGGAGGAGA TCCGTCTGAG AACGGAACTT 
GAACTGGTGC GCACTGAGAA CACGGAGCTT GAGTCGGAGC AGCAAAAGGC TCACATCCAA 
ATCACAGAGC TTCTCGAACA GAACATTAAG CTCAACCAGG AACTGGCCCA AACGTCGAGC 
AGCATTGGTG GCACCCCGGA GCACAGTCCA TTGCGACCGA GAAGGCATAG CGAGGACAAG 
GAGGAGGAGA TGCTCCAGCT AATGGAGAAG CTGGCTGCTC TTCAAATGGA GAACGCCCAG 
CTGCGTGACA AGACTGACGA ACTGACCATC GAAATCGAGA GCTTAAATGT GGAACTAATT 
CGCTCGAAAA CCAAGGCTAA AAAGCAAGAA AAACAGGAGA AACAAGAGGA CCAGGAGTCG 
GCGGCCACGG CTACCAAAAG GCGTGGGGAT TCGCCGAGCA AAACACATCT AACAGAGGAG 
AGCCCTCGCT TGGGGAAACA GCGCAAGTGC ACCGAAGGAG AGCAGAGCGA TGCCAGCAAC 
AGCGGAGATT GGTTGGCTCT AAACTCCGAG CTGCAAAGAA GTCAAAGCCA GGATGAGGAG 
CTAACAAGCC TTAGACAGCG GGTTGCTGAG CTAGAGGAGG AACTCAAGGC TGCAAAGGAA 
GGCAGATCTC TCACCCCGGA AAGCCGTTCG AAGGAACTGG AGACCAGTCT AGAGCAAATG 
CAGCGTGCCT ATGAGGATTG CGAGGACTAC TGGCAAACGA AACTTAGCGA GGAGCGGCAG 
CTGTTTGAGA AGGAGCGACA GATCTACGAA GATGAGCAGC ACGAGAGCGA CAAGAAGTTC 
ACCGAGCTGA TGGAAAAGGT GCGCGAGTAC GAGGAGCAGT TCAGCAAGGA TGGCCGCCTC 
TCGCCCATTG ATGAGCGCGA TATGCTGGAA CAGCAGTACT CGGAATTGGA GGCAGAGGCA 
GCCCAGCTGC GCTCGAGTTC CATTCAAATG CTCGAGGAGA AGGCTCAGGA AATCAGTTCA 
CTGCAATCAG AGATCGAGGA TTTGCGACAG AGATTGGGTG AGAGCGTTGA GATCCTCACA 
GGCGCCTGTG AACTCACCTC GGAGTCGGTA GCCCAACTGA GTGCCGAGGC GGGAAAAAGT 
CCAGCCAGCT CACCCATCAG CTACCTCTGG CTGCAGAGCA CCATCCAAGA GCCAGCGAAA 
TCGCTTGCCG ATTCCAAGGA TGAAGCCACC GCCAGTGCCA TCGAATTGCT CGGAGGCTCA 
CCATCGCACA AGACAGCCAG CCGCACTACA CCCTTCGAAA GCTCTCAGTC GGGTCCTTCG 
CCCACGAACA GTGGCAACAG CAACGCCTAC GGCGCCAATC CCGGCCCAGC TCCGATCAGC 
AAGCCCAAGC GGTCCCAGAG TCCCCAACAG GCGGCTGCAT CGGAGGGAGA GATAGCCGAT 
TGCGAGACGT CGTCGACGGC GTCCGGCAAA AGCTTCGAAT CCAACAGTAA AACGTCTTGC 
CTTAGCCACG AGAAGTGCAG CAGTCCGTCG GCACTGAAGG AGGAACTGAA GCGCCTTAAG 
TTCTTCGAGC TCTCCCTCAA GGAGCAAATC AAGGATCTGA GTCTGCAGCG GGACGGTCTG 
GTCATGGAAC TGCAGCAGTT GCAGGAGGCG CGACCCGTGC TCGAGAAGGC CTATGCGCGA 
ACAACGCATC CAACGCTTCA GCAGCGACTG AACCAATTGG AGCTGCGAAA TCGCCATCTG 
CAGAATGTCA TCAAGCAGCA GCAGCATTAC ACGGAGTCCC TGATGCAGCA ATCCTGGCGG 
CAGCATCAAG TGGAGCTCAA CGATTTGCAT AGCCGAATCG AGACCCAGGG TGTTTTACTG 
GCCGATCAGA CACAGCGATT GCAGAGTGCC GACATCCTGG TGAAGGATCT ATATGTGGAG 
AACTCCCATC TGACGGCTAC GGTGCAGCGG TTGGAGCAGC AACGAGCTAG GGTGAACCTC 
ATTCACCAGC AGCAGCAACA GCAGCGCCTT GTGGGCGGTG GACTGCCTGG CATGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188699.1
CDS	360..3599
Translation	MEVSADPYEQKLYQMFRSCETQCGLLDEKSLLKLCSLLELRDQGSALIASLGGSHQLGVSFGQFKEALLNFLGSEFDGNTSSGFIERSLVITDEPLNNTYIESPPESSDREVSPKLVVGTKKYGRRSRPQQGIYELSVTDSDNTDEDQLQQQQNQRSLNGCDELGVQVQRSSSQSDLPGSRRLRSVHTSGSKLKRCASLPARRKMNSNTTGATTSPTAAAKLKQLSIQSQAQHSSSVESLDTVTPQQLETISVHSIMEAWELASIPNTRNLLHVLGFDEEEEVNLQQLTKALEEELRGIDGDHEQSNMLRALAALQATELGNYRLAYRQQHEENLKLRADNKAANQRVALLAVEVDERHASLEDNSKKQVQQLEQRHASMVREITLRMTNDRDHWTSMTGKLEAQLKSLEQEEIRLRTELELVRTENTELESEQQKAHIQITELLEQNIKLNQELAQTSSSIGGTPEHSPLRPRRHSEDKEEEMLQLMEKLAALQMENAQLRDKTDELTIEIESLNVELIRSKTKAKKQEKQEKQEDQESAATATKRRGDSPSKTHLTEESPRLGKQRKCTEGEQSDASNSGDWLALNSELQRSQSQDEELTSLRQRVAELEEELKAAKEGRSLTPESRSKELETSLEQMQRAYEDCEDYWQTKLSEERQLFEKERQIYEDEQHESDKKFTELMEKVREYEEQFSKDGRLSPIDERDMLEQQYSELEAEAAQLRSSSIQMLEEKAQEISSLQSEIEDLRQRLGESVEILTGACELTSESVAQLSAEAGKSPASSPISYLWLQSTIQEPAKSLADSKDEATASAIELLGGSPSHKTASRTTPFESSQSGPSPTNSGNSNAYGANPGPAPISKPKRSQSPQQAAASEGEIADCETSSTASGKSFESNSKTSCLSHEKCSSPSALKEELKRLKFFELSLKEQIKDLSLQRDGLVMELQQLQEARPVLEKAYARTTHPTLQQRLNQLELRNRHLQNVIKQQQHYTESLMQQSWRQHQVELNDLHSRIETQGVLLADQTQRLQSADILVKDLYVENSHLTATVQRLEQQRARVNLIHQQQQQQRLVGGGLPGMP

Target ORF information:

RefSeq Version	NM_001201770.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201770.2

ORF Insert Sequence:

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGGTAATACG 
TCATCGGGTT TCATTGAGCG TTCACTGGTG ATTACGGATG AGCCGCTAAA CAACACATAC 
ATCGAGAGTC CGCCGGAGTC TTCCGATCGC GAGGTTTCAC CCAAACTCGT CGTGGGCACC 
AAGAAATACG GTCGCCGGTC TAGGCCACAG CAGGGAATCT ACGAGTTATC CGTCACGGAC 
TCGGACAATA CGGACGAGGA CCAGTTGCAG CAGCAGCAAA ATCAGCGAAG CCTCAACGGA 
TGCGATGAGC TGGGAGTTCA GGTGCAACGT TCCTCGTCCC AGAGCGATCT TCCTGGCAGC 
CGGCGTCTGC GGTCCGTCCA CACCAGCGGG AGCAAACTGA AGCGTTGTGC TTCACTGCCA 
GCACGCCGGA AGATGAACAG CAACACCACG GGAGCCACTA CATCACCGAC GGCAGCGGCC 
AAGTTGAAAC AGCTTTCCAT CCAGAGCCAG GCGCAGCACA GCAGCAGCGT GGAATCACTG 
GACACCGTGA CGCCGCAGCA ATTGGAGACG ATCTCAGTGC ATAGCATTAT GGAAGCCTGG 
GAGCTGGCCA GCATTCCCAA CACTCGCAAC CTACTTCACG TCCTGGGATT CGATGAGGAG 
GAGGAGGTGA ACCTGCAGCA GCTAACTAAG GCATTGGAGG AGGAGCTGCG GGGCATCGAT 
GGGGATCACG AGCAATCGAA TATGTTGCGC GCTCTGGCTG CTCTGCAGGC CACCGAGTTG 
GGCAACTACA GACTTGCCTA TAGGCAGCAG CATGAGGAGA ACCTCAAGCT GAGGGCCGAT 
AATAAGGCGG CCAACCAAAG GGTGGCTTTG CTTGCCGTGG AAGTGGATGA GCGGCATGCG 
TCGCTGGAGG ATAACTCCAA GAAGCAGGTG CAGCAGCTGG AGCAAAGACA CGCCAGCATG 
GTGCGTGAAA TAACGCTGCG GATGACTAAT GACCGCGATC ACTGGACCAG CATGACGGGA 
AAGCTGGAGG CACAGCTTAA ATCGCTTGAG CAGGAGGAGA TCCGTCTGAG AACGGAACTT 
GAACTGGTGC GCACTGAGAA CACGGAGCTT GAGTCGGAGC AGCAAAAGGC TCACATCCAA 
ATCACAGAGC TTCTCGAACA GAACATTAAG CTCAACCAGG AACTGGCCCA AACGTCGAGC 
AGCATTGGTG GCACCCCGGA GCACAGTCCA TTGCGACCGA GAAGGCATAG CGAGGACAAG 
GAGGAGGAGA TGCTCCAGCT AATGGAGAAG CTGGCTGCTC TTCAAATGGA GAACGCCCAG 
CTGCGTGACA AGACTGACGA ACTGACCATC GAAATCGAGA GCTTAAATGT GGAACTAATT 
CGCTCGAAAA CCAAGGCTAA AAAGCAAGAA AAACAGGAGA AACAAGAGGA CCAGGAGTCG 
GCGGCCACGG CTACCAAAAG GCGTGGGGAT TCGCCGAGCA AAACACATCT AACAGAGGAG 
AGCCCTCGCT TGGGGAAACA GCGCAAGTGC ACCGAAGGAG AGCAGAGCGA TGCCAGCAAC 
AGCGGAGATT GGTTGGCTCT AAACTCCGAG CTGCAAAGAA GTCAAAGCCA GGATGAGGAG 
CTAACAAGCC TTAGACAGCG GGTTGCTGAG CTAGAGGAGG AACTCAAGGC TGCAAAGGAA 
GGCAGATCTC TCACCCCGGA AAGCCGTTCG AAGGAACTGG AGACCAGTCT AGAGCAAATG 
CAGCGTGCCT ATGAGGATTG CGAGGACTAC TGGCAAACGA AACTTAGCGA GGAGCGGCAG 
CTGTTTGAGA AGGAGCGACA GATCTACGAA GATGAGCAGC ACGAGAGCGA CAAGAAGTTC 
ACCGAGCTGA TGGAAAAGGT GCGCGAGTAC GAGGAGCAGT TCAGCAAGGA TGGCCGCCTC 
TCGCCCATTG ATGAGCGCGA TATGCTGGAA CAGCAGTACT CGGAATTGGA GGCAGAGGCA 
GCCCAGCTGC GCTCGAGTTC CATTCAAATG CTCGAGGAGA AGGCTCAGGA AATCAGTTCA 
CTGCAATCAG AGATCGAGGA TTTGCGACAG AGATTGGGTG AGAGCGTTGA GATCCTCACA 
GGCGCCTGTG AACTCACCTC GGAGTCGGTA GCCCAACTGA GTGCCGAGGC GGGAAAAAGT 
CCAGCCAGCT CACCCATCAG CTACCTCTGG CTGCAGAGCA CCATCCAAGA GCCAGCGAAA 
TCGCTTGCCG ATTCCAAGGA TGAAGCCACC GCCAGTGCCA TCGAATTGCT CGGAGGCTCA 
CCATCGCACA AGACAGCCAG CCGCACTACA CCCTTCGAAA GCTCTCAGTC GGGTCCTTCG 
CCCACGAACA GTGGCAACAG CAACGCCTAC GGCGCCAATC CCGGCCCAGC TCCGATCAGC 
AAGCCCAAGC GGTCCCAGAG TCCCCAACAG GCGGCTGCAT CGGAGGGAGA GATAGCCGAT 
TGCGAGACGT CGTCGACGGC GTCCGGCAAA AGCTTCGAAT CCAACAGTAA AACGTCTTGC 
CTTAGCCACG AGAAGTGCAG CAGTCCGTCG GCACTGAAGG AGGAACTGAA GCGCCTTAAG 
TTCTTCGAGC TCTCCCTCAA GGAGCAAATC AAGGATCTGA GTCTGCAGCG GGACGGTCTG 
GTCATGGAAC TGCAGCAGTT GCAGGAGGCG CGACCCGTGC TCGAGAAGGC CTATGCGCGA 
ACAACGCATC CAACGCTTCA GCAGCGACTG AACCAATTGG AGCTGCGAAA TCGCCATCTG 
CAGAATGTCA TCAAGCAGCA GCAGCATTAC ACGGAGTCCC TGATGCAGCA ATCCTGGCGG 
CAGCATCAAG TGGAGCTCAA CGATTTGCAT AGCCGAATCG AGACCCAGGG TGTTTTACTG 
GCCGATCAGA CACAGCGATT GCAGAGTGCC GACATCCTGG TGAAGGATCT ATATGTGGAG 
AACTCCCATC TGACGGCTAC GGTGCAGCGG TTGGAGCAGC AACGAGCTAG GGTGAACCTC 
ATTCACCAGC AGCAGCAACA GCAGCGCCTT GTGGGCGGTG GACTGCCTGG CATGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bsg25D ( NM_001273164.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260093.1 Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa13794
Clone ID Related Accession (Same CDS sequence)	NM_001273164.2
Accession Version	NM_001273164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blastoderm-specific gene 25D, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273164.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGGTAATACG 
TCATCGGGTT TCATTGAGCG TTCACTGGTG ATTACGGATG AGCCGCTAAA CAACACATAC 
ATCGAGAGTC CGCCGGAGTC TTCCGATCGC GAGGTTTCAC CCAAACTCGT CGTGGGCACC 
AAGAAATACG GTCGCCGGTC TAGGCCACAG CAGGGAATCT ACGAGTTATC CGTCACGGAC 
TCGGACAATA CGGACGAGGA CCAGTTGCAG CAGCAGCAAA ATCAGCGAAG CCTCAACGGA 
TGCGATGAGC TGGGAGTTCA GGTGCAACGT TCCTCGTCCC AGAGCGATCT TCCTGGCAGC 
CGGCGTCTGC GGTCCGTCCA CACCAGCGGG AGCAAACTGA AGCGTTGTGC TTCACTGCCA 
GCACGCCGGA AGATGAACAG CAACACCACG GGAGCCACTA CATCACCGAC GGCAGCGGCC 
AAGTTGAAAC AGCTTTCCAT CCAGAGCCAG GCGCAGCACA GCAGCAGCGT GGAATCACTG 
GGCCATCGCC TGGCCACCAA TCCCGCACCG TCGTCCGAGC ACTGGAGATG CTACCACGGC 
GGCCGTTGGT CATGCTGCAA AGACACCGTG ACGCCGCAGC AATTGGAGAC GATCTCAGTG 
CATAGCATTA TGGAAGCCTG GGAGCTGGCC AGCATTCCCA ACACTCGCAA CCTACTTCAC 
GTCCTGGGAT TCGATGAGGA GGAGGAGGTG AACCTGCAGC AGCTAACTAA GGCATTGGAG 
GAGGAGCTGC GGGGCATCGA TGGGGATCAC GAGCAATCGA ATATGTTGCG CGCTCTGGCT 
GCTCTGCAGG CCACCGAGTT GGGCAACTAC AGACTTGCCT ATAGGCAGCA GCATGAGGAG 
AACCTCAAGC TGAGGGCCGA TAATAAGGCG GCCAACCAAA GGGTGGCTTT GCTTGCCGTG 
GAAGTGGATG AGCGGCATGC GTCGCTGGAG GATAACTCCA AGAAGCAGGT GCAGCAGCTG 
GAGCAAAGAC ACGCCAGCAT GGTGCGTGAA ATAACGCTGC GGATGACTAA TGACCGCGAT 
CACTGGACCA GCATGACGGG AAAGCTGGAG GCACAGCTTA AATCGCTTGA GCAGGAGGAG 
ATCCGTCTGA GAACGGAACT TGAACTGGTG CGCACTGAGA ACACGGAGCT TGAGTCGGAG 
CAGCAAAAGG CTCACATCCA AATCACAGAG CTTCTCGAAC AGAACATTAA GCTCAACCAG 
GAACTGGCCC AAACGTCGAG CAGCATTGGT GGCACCCCGG AGCACAGTCC ATTGCGACCG 
AGAAGGCATA GCGAGGACAA GGAGGAGGAG ATGCTCCAGC TAATGGAGAA GCTGGCTGCT 
CTTCAAATGG AGAACGCCCA GCTGCGTGAC AAGACTGACG AACTGACCAT CGAAATCGAG 
AGCTTAAATG TGGAACTAAT TCGCTCGAAA ACCAAGGCTA AAAAGCAAGA AAAACAGGAG 
AAACAAGAGG ACCAGGAGTC GGCGGCCACG GCTACCAAAA GGCGTGGGGA TTCGCCGAGC 
AAAACACATC TAACAGAGGA GAGCCCTCGC TTGGGGAAAC AGCGCAAGTG CACCGAAGGA 
GAGCAGAGCG ATGCCAGCAA CAGCGGAGAT TGGTTGGCTC TAAACTCCGA GCTGCAAAGA 
AGTCAAAGCC AGGATGAGGA GCTAACAAGC CTTAGACAGC GGGTTGCTGA GCTAGAGGAG 
GAACTCAAGG CTGCAAAGGA AGGCAGATCT CTCACCCCGG AAAGCCGTTC GAAGGAACTG 
GAGACCAGTC TAGAGCAAAT GCAGCGTGCC TATGAGGATT GCGAGGACTA CTGGCAAACG 
AAACTTAGCG AGGAGCGGCA GCTGTTTGAG AAGGAGCGAC AGATCTACGA AGATGAGCAG 
CACGAGAGCG ACAAGAAGTT CACCGAGCTG ATGGAAAAGG TGCGCGAGTA CGAGGAGCAG 
TTCAGCAAGG ATGGCCGCCT CTCGCCCATT GATGAGCGCG ATATGCTGGA ACAGCAGTAC 
TCGGAATTGG AGGCAGAGGC AGCCCAGCTG CGCTCGAGTT CCATTCAAAT GCTCGAGGAG 
AAGGCTCAGG AAATCAGTTC ACTGCAATCA GAGATCGAGG ATTTGCGACA GAGATTGGGT 
GAGAGCGTTG AGATCCTCAC AGGCGCCTGT GAACTCACCT CGGAGTCGGT AGCCCAACTG 
AGTGCCGAGG CGGGAAAAAG TCCAGCCAGC TCACCCATCA GCTACCTCTG GCTGCAGAGC 
ACCATCCAAG AGCCAGCGAA ATCGCTTGCC GATTCCAAGG ATGAAGCCAC CGCCAGTGCC 
ATCGAATTGC TCGGAGGCTC ACCATCGCAC AAGACAGCCA GCCGCAATAA CCTCACCACT 
TCGGAAACAT CCATCTTTAG CACTACACCC TTCGAAAGCT CTCAGTCGGG TCCTTCGCCC 
ACGAACAGTG GCAACAGCAA CGCCTACGGC GCCAATCCCG GCCCAGCTCC GATCAGCAAG 
CCCAAGCGGT CCCAGAGTCC CCAACAGGCG GCTGCATCGG AGGGAGAGAT AGCCGATTGC 
GAGACGTCGT CGACGGCGTC CGGCAAAAGC TTCGAATCCA ACAGTAAAAC GTCTTGCCTT 
AGCCACGAGA AGTGCAGCAG TCCGTCGGCA CTGAAGGAGG AACTGAAGCG CCTTAAGTTC 
TTCGAGCTCT CCCTCAAGGA GCAAATCAAG GATCTGAGTC TGCAGCGGGA CGGTCTGGTC 
ATGGAACTGC AGCAGTTGCA GGAGGCGCGA CCCGTGCTCG AGAAGGCCTA TGCGCGAACA 
ACGCATCCAA CGCTTCAGCA GCGACTGAAC CAATTGGAGC TGCGAAATCG CCATCTGCAG 
AATGTCATCA AGCAGCAGCA GCATTACACG GAGTCCCTGA TGCAGCAATC CTGGCGGCAG 
CATCAAGTGG AGCTCAACGA TTTGCATAGC CGAATCGAGA CCCAGGGTGT TTTACTGGCC 
GATCAGACAC AGCGATTGCA GAGTGCCGAC ATCCTGGTGA AGGATCTATA TGTGGAGAAC 
TCCCATCTGA CGGCTACGGT GCAGCGGTTG GAGCAGCAAC GAGCTAGGGT GAACCTCATT 
CACCAGCAGC AGCAACAGCA GCGCCTTGTG GGCGGTGGAC TGCCTGGCAT GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260093.1
CDS	360..3716
Translation	MEVSADPYEQKLYQMFRSCETQCGLLDEKSLLKLCSLLELRDQGSALIASLGGSHQLGVSFGQFKEALLNFLGSEFDGNTSSGFIERSLVITDEPLNNTYIESPPESSDREVSPKLVVGTKKYGRRSRPQQGIYELSVTDSDNTDEDQLQQQQNQRSLNGCDELGVQVQRSSSQSDLPGSRRLRSVHTSGSKLKRCASLPARRKMNSNTTGATTSPTAAAKLKQLSIQSQAQHSSSVESLGHRLATNPAPSSEHWRCYHGGRWSCCKDTVTPQQLETISVHSIMEAWELASIPNTRNLLHVLGFDEEEEVNLQQLTKALEEELRGIDGDHEQSNMLRALAALQATELGNYRLAYRQQHEENLKLRADNKAANQRVALLAVEVDERHASLEDNSKKQVQQLEQRHASMVREITLRMTNDRDHWTSMTGKLEAQLKSLEQEEIRLRTELELVRTENTELESEQQKAHIQITELLEQNIKLNQELAQTSSSIGGTPEHSPLRPRRHSEDKEEEMLQLMEKLAALQMENAQLRDKTDELTIEIESLNVELIRSKTKAKKQEKQEKQEDQESAATATKRRGDSPSKTHLTEESPRLGKQRKCTEGEQSDASNSGDWLALNSELQRSQSQDEELTSLRQRVAELEEELKAAKEGRSLTPESRSKELETSLEQMQRAYEDCEDYWQTKLSEERQLFEKERQIYEDEQHESDKKFTELMEKVREYEEQFSKDGRLSPIDERDMLEQQYSELEAEAAQLRSSSIQMLEEKAQEISSLQSEIEDLRQRLGESVEILTGACELTSESVAQLSAEAGKSPASSPISYLWLQSTIQEPAKSLADSKDEATASAIELLGGSPSHKTASRNNLTTSETSIFSTTPFESSQSGPSPTNSGNSNAYGANPGPAPISKPKRSQSPQQAAASEGEIADCETSSTASGKSFESNSKTSCLSHEKCSSPSALKEELKRLKFFELSLKEQIKDLSLQRDGLVMELQQLQEARPVLEKAYARTTHPTLQQRLNQLELRNRHLQNVIKQQQHYTESLMQQSWRQHQVELNDLHSRIETQGVLLADQTQRLQSADILVKDLYVENSHLTATVQRLEQQRARVNLIHQQQQQQRLVGGGLPGMP

Target ORF information:

RefSeq Version	NM_001273164.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273164.2

ORF Insert Sequence:

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGGTAATACG 
TCATCGGGTT TCATTGAGCG TTCACTGGTG ATTACGGATG AGCCGCTAAA CAACACATAC 
ATCGAGAGTC CGCCGGAGTC TTCCGATCGC GAGGTTTCAC CCAAACTCGT CGTGGGCACC 
AAGAAATACG GTCGCCGGTC TAGGCCACAG CAGGGAATCT ACGAGTTATC CGTCACGGAC 
TCGGACAATA CGGACGAGGA CCAGTTGCAG CAGCAGCAAA ATCAGCGAAG CCTCAACGGA 
TGCGATGAGC TGGGAGTTCA GGTGCAACGT TCCTCGTCCC AGAGCGATCT TCCTGGCAGC 
CGGCGTCTGC GGTCCGTCCA CACCAGCGGG AGCAAACTGA AGCGTTGTGC TTCACTGCCA 
GCACGCCGGA AGATGAACAG CAACACCACG GGAGCCACTA CATCACCGAC GGCAGCGGCC 
AAGTTGAAAC AGCTTTCCAT CCAGAGCCAG GCGCAGCACA GCAGCAGCGT GGAATCACTG 
GGCCATCGCC TGGCCACCAA TCCCGCACCG TCGTCCGAGC ACTGGAGATG CTACCACGGC 
GGCCGTTGGT CATGCTGCAA AGACACCGTG ACGCCGCAGC AATTGGAGAC GATCTCAGTG 
CATAGCATTA TGGAAGCCTG GGAGCTGGCC AGCATTCCCA ACACTCGCAA CCTACTTCAC 
GTCCTGGGAT TCGATGAGGA GGAGGAGGTG AACCTGCAGC AGCTAACTAA GGCATTGGAG 
GAGGAGCTGC GGGGCATCGA TGGGGATCAC GAGCAATCGA ATATGTTGCG CGCTCTGGCT 
GCTCTGCAGG CCACCGAGTT GGGCAACTAC AGACTTGCCT ATAGGCAGCA GCATGAGGAG 
AACCTCAAGC TGAGGGCCGA TAATAAGGCG GCCAACCAAA GGGTGGCTTT GCTTGCCGTG 
GAAGTGGATG AGCGGCATGC GTCGCTGGAG GATAACTCCA AGAAGCAGGT GCAGCAGCTG 
GAGCAAAGAC ACGCCAGCAT GGTGCGTGAA ATAACGCTGC GGATGACTAA TGACCGCGAT 
CACTGGACCA GCATGACGGG AAAGCTGGAG GCACAGCTTA AATCGCTTGA GCAGGAGGAG 
ATCCGTCTGA GAACGGAACT TGAACTGGTG CGCACTGAGA ACACGGAGCT TGAGTCGGAG 
CAGCAAAAGG CTCACATCCA AATCACAGAG CTTCTCGAAC AGAACATTAA GCTCAACCAG 
GAACTGGCCC AAACGTCGAG CAGCATTGGT GGCACCCCGG AGCACAGTCC ATTGCGACCG 
AGAAGGCATA GCGAGGACAA GGAGGAGGAG ATGCTCCAGC TAATGGAGAA GCTGGCTGCT 
CTTCAAATGG AGAACGCCCA GCTGCGTGAC AAGACTGACG AACTGACCAT CGAAATCGAG 
AGCTTAAATG TGGAACTAAT TCGCTCGAAA ACCAAGGCTA AAAAGCAAGA AAAACAGGAG 
AAACAAGAGG ACCAGGAGTC GGCGGCCACG GCTACCAAAA GGCGTGGGGA TTCGCCGAGC 
AAAACACATC TAACAGAGGA GAGCCCTCGC TTGGGGAAAC AGCGCAAGTG CACCGAAGGA 
GAGCAGAGCG ATGCCAGCAA CAGCGGAGAT TGGTTGGCTC TAAACTCCGA GCTGCAAAGA 
AGTCAAAGCC AGGATGAGGA GCTAACAAGC CTTAGACAGC GGGTTGCTGA GCTAGAGGAG 
GAACTCAAGG CTGCAAAGGA AGGCAGATCT CTCACCCCGG AAAGCCGTTC GAAGGAACTG 
GAGACCAGTC TAGAGCAAAT GCAGCGTGCC TATGAGGATT GCGAGGACTA CTGGCAAACG 
AAACTTAGCG AGGAGCGGCA GCTGTTTGAG AAGGAGCGAC AGATCTACGA AGATGAGCAG 
CACGAGAGCG ACAAGAAGTT CACCGAGCTG ATGGAAAAGG TGCGCGAGTA CGAGGAGCAG 
TTCAGCAAGG ATGGCCGCCT CTCGCCCATT GATGAGCGCG ATATGCTGGA ACAGCAGTAC 
TCGGAATTGG AGGCAGAGGC AGCCCAGCTG CGCTCGAGTT CCATTCAAAT GCTCGAGGAG 
AAGGCTCAGG AAATCAGTTC ACTGCAATCA GAGATCGAGG ATTTGCGACA GAGATTGGGT 
GAGAGCGTTG AGATCCTCAC AGGCGCCTGT GAACTCACCT CGGAGTCGGT AGCCCAACTG 
AGTGCCGAGG CGGGAAAAAG TCCAGCCAGC TCACCCATCA GCTACCTCTG GCTGCAGAGC 
ACCATCCAAG AGCCAGCGAA ATCGCTTGCC GATTCCAAGG ATGAAGCCAC CGCCAGTGCC 
ATCGAATTGC TCGGAGGCTC ACCATCGCAC AAGACAGCCA GCCGCAATAA CCTCACCACT 
TCGGAAACAT CCATCTTTAG CACTACACCC TTCGAAAGCT CTCAGTCGGG TCCTTCGCCC 
ACGAACAGTG GCAACAGCAA CGCCTACGGC GCCAATCCCG GCCCAGCTCC GATCAGCAAG 
CCCAAGCGGT CCCAGAGTCC CCAACAGGCG GCTGCATCGG AGGGAGAGAT AGCCGATTGC 
GAGACGTCGT CGACGGCGTC CGGCAAAAGC TTCGAATCCA ACAGTAAAAC GTCTTGCCTT 
AGCCACGAGA AGTGCAGCAG TCCGTCGGCA CTGAAGGAGG AACTGAAGCG CCTTAAGTTC 
TTCGAGCTCT CCCTCAAGGA GCAAATCAAG GATCTGAGTC TGCAGCGGGA CGGTCTGGTC 
ATGGAACTGC AGCAGTTGCA GGAGGCGCGA CCCGTGCTCG AGAAGGCCTA TGCGCGAACA 
ACGCATCCAA CGCTTCAGCA GCGACTGAAC CAATTGGAGC TGCGAAATCG CCATCTGCAG 
AATGTCATCA AGCAGCAGCA GCATTACACG GAGTCCCTGA TGCAGCAATC CTGGCGGCAG 
CATCAAGTGG AGCTCAACGA TTTGCATAGC CGAATCGAGA CCCAGGGTGT TTTACTGGCC 
GATCAGACAC AGCGATTGCA GAGTGCCGAC ATCCTGGTGA AGGATCTATA TGTGGAGAAC 
TCCCATCTGA CGGCTACGGT GCAGCGGTTG GAGCAGCAAC GAGCTAGGGT GAACCTCATT 
CACCAGCAGC AGCAACAGCA GCGCCTTGTG GGCGGTGGAC TGCCTGGCAT GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bsg25D ( NM_164637.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723072.1 Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16260
Clone ID Related Accession (Same CDS sequence)	NM_164637.3
Accession Version	NM_164637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blastoderm-specific gene 25D, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164637.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGGTAATACG 
TCATCGGGTT TCATTGAGCG TTCACTGGTG ATTACGGATG AGCCGCTAAA CAACACATAC 
ATCGAGAGTC CGCCGGAGTC TTCCGATCGC GAGGTTTCAC CCAAACTCGT CGTGGGCACC 
AAGAAATACG GTCGCCGGTC TAGGCCACAG CAGGGAATCT ACGAGTTATC CGTCACGGAC 
TCGGACAATA CGGACGAGGA CCAGTTGCAG CAGCAGCAAA ATCAGCGAAG CCTCAACGGA 
TGCGATGAGC TGGGAGTTCA GGTGCAACGT TCCTCGTCCC AGAGCGATCT TCCTGGCAGC 
CGGCGTCTGC GGTCCGTCCA CACCAGCGGG AGCAAACTGA AGCGTTGTGC TTCACTGCCA 
GCACGCCGGA AGATGAACAG CAACACCACG GGAGCCACTA CATCACCGAC GGCAGCGGCC 
AAGTTGAAAC AGCTTTCCAT CCAGAGCCAG GCGCAGCACA GCAGCAGCGT GGAATCACTG 
GACACCGTGA CGCCGCAGCA ATTGGAGACG ATCTCAGTGC ATAGCATTAT GGAAGCCTGG 
GAGCTGGCCA GCATTCCCAA CACTCGCAAC CTACTTCACG TCCTGGGATT CGATGAGGAG 
GAGGAGGTGA ACCTGCAGCA GCTAACTAAG GCATTGGAGG AGGAGCTGCG GGGCATCGAT 
GGGGATCACG AGCAATCGAA TATGTTGCGC GCTCTGGCTG CTCTGCAGGC CACCGAGTTG 
GGCAACTACA GACTTGCCTA TAGGCAGCAG CATGAGGAGA ACCTCAAGCT GAGGGCCGAT 
AATAAGGCGG CCAACCAAAG GGTGGCTTTG CTTGCCGTGG AAGTGGATGA GCGGCATGCG 
TCGCTGGAGG ATAACTCCAA GAAGCAGGTG CAGCAGCTGG AGCAAAGACA CGCCAGCATG 
GTGCGTGAAA TAACGCTGCG GATGACTAAT GACCGCGATC ACTGGACCAG CATGACGGGA 
AAGCTGGAGG CACAGCTTAA ATCGCTTGAG CAGGAGGAGA TCCGTCTGAG AACGGAACTT 
GAACTGGTGC GCACTGAGAA CACGGAGCTT GAGTCGGAGC AGCAAAAGGC TCACATCCAA 
ATCACAGAGC TTCTCGAACA GAACATTAAG CTCAACCAGG AACTGGCCCA AACGTCGAGC 
AGCATTGGTG GCACCCCGGA GCACAGTCCA TTGCGACCGA GAAGGCATAG CGAGGACAAG 
GAGGAGGAGA TGCTCCAGCT AATGGAGAAG CTGGCTGCTC TTCAAATGGA GAACGCCCAG 
CTGCGTGACA AGACTGACGA ACTGACCATC GAAATCGAGA GCTTAAATGT GGAACTAATT 
CGCTCGAAAA CCAAGGCTAA AAAGCAAGAA AAACAGGAGA AACAAGAGGA CCAGGAGTCG 
GCGGCCACGG CTACCAAAAG GCGTGGGGAT TCGCCGAGCA AAACACATCT AACAGAGGAG 
AGCCCTCGCT TGGGGAAACA GCGCAAGTGC ACCGAAGGAG AGCAGAGCGA TGCCAGCAAC 
AGCGGAGATT GGTTGGCTCT AAACTCCGAG CTGCAAAGAA GTCAAAGCCA GGATGAGGAG 
CTAACAAGCC TTAGACAGCG GGTTGCTGAG CTAGAGGAGG AACTCAAGGC TGCAAAGGAA 
GGCAGATCTC TCACCCCGGA AAGCCGTTCG AAGGAACTGG AGACCAGTCT AGAGCAAATG 
CAGCGTGCCT ATGAGGATTG CGAGGACTAC TGGCAAACGA AACTTAGCGA GGAGCGGCAG 
CTGTTTGAGA AGGAGCGACA GATCTACGAA GATGAGCAGC ACGAGAGCGA CAAGAAGTTC 
ACCGAGCTGA TGGAAAAGGT GCGCGAGTAC GAGGAGCAGT TCAGCAAGGA TGGCCGCCTC 
TCGCCCATTG ATGAGCGCGA TATGCTGGAA CAGCAGTACT CGGAATTGGA GGCAGAGGCA 
GCCCAGCTGC GCTCGAGTTC CATTCAAATG CTCGAGGAGA AGGCTCAGGA AATCAGTTCA 
CTGCAATCAG AGATCGAGGA TTTGCGACAG AGATTGGGTG AGAGCGTTGA GATCCTCACA 
GGCGCCTGTG AACTCACCTC GGAGTCGGTA GCCCAACTGA GTGCCGAGGC GGGAAAAAGT 
CCAGCCAGCT CACCCATCAG CTACCTCTGG CTGCAGAGCA CCATCCAAGA GCCAGCGAAA 
TCGCTTGCCG ATTCCAAGGA TGAAGCCACC GCCAGTGCCA TCGAATTGCT CGGAGGCTCA 
CCATCGCACA AGACAGCCAG CCGCAATAAC CTCACCACTT CGGAAACATC CATCTTTAGC 
ACTACACCCT TCGAAAGCTC TCAGTCGGGT CCTTCGCCCA CGAACAGTGG CAACAGCAAC 
GCCTACGGCG CCAATCCCGG CCCAGCTCCG ATCAGCAAGC CCAAGCGGTC CCAGAGTCCC 
CAACAGGCGG CTGCATCGGA GGGAGAGATA GCCGATTGCG AGACGTCGTC GACGGCGTCC 
GGCAAAAGCT TCGAATCCAA CAGTAAAACG TCTTGCCTTA GCCACGAGAA GTGCAGCAGT 
CCGTCGGCAC TGAAGGAGGA ACTGAAGCGC CTTAAGTTCT TCGAGCTCTC CCTCAAGGAG 
CAAATCAAGG ATCTGAGTCT GCAGCGGGAC GGTCTGGTCA TGGAACTGCA GCAGTTGCAG 
GAGGCGCGAC CCGTGCTCGA GAAGGCCTAT GCGCGAACAA CGCATCCAAC GCTTCAGCAG 
CGACTGAACC AATTGGAGCT GCGAAATCGC CATCTGCAGA ATGTCATCAA GCAGCAGCAG 
CATTACACGG AGTCCCTGAT GCAGCAATCC TGGCGGCAGC ATCAAGTGGA GCTCAACGAT 
TTGCATAGCC GAATCGAGAC CCAGGGTGTT TTACTGGCCG ATCAGACACA GCGATTGCAG 
AGTGCCGACA TCCTGGTGAA GGATCTATAT GTGGAGAACT CCCATCTGAC GGCTACGGTG 
CAGCGGTTGG AGCAGCAACG AGCTAGGGTG AACCTCATTC ACCAGCAGCA GCAACAGCAG 
CGCCTTGTGG GCGGTGGACT GCCTGGCATG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723072.1
CDS	360..3635
Translation	MEVSADPYEQKLYQMFRSCETQCGLLDEKSLLKLCSLLELRDQGSALIASLGGSHQLGVSFGQFKEALLNFLGSEFDGNTSSGFIERSLVITDEPLNNTYIESPPESSDREVSPKLVVGTKKYGRRSRPQQGIYELSVTDSDNTDEDQLQQQQNQRSLNGCDELGVQVQRSSSQSDLPGSRRLRSVHTSGSKLKRCASLPARRKMNSNTTGATTSPTAAAKLKQLSIQSQAQHSSSVESLDTVTPQQLETISVHSIMEAWELASIPNTRNLLHVLGFDEEEEVNLQQLTKALEEELRGIDGDHEQSNMLRALAALQATELGNYRLAYRQQHEENLKLRADNKAANQRVALLAVEVDERHASLEDNSKKQVQQLEQRHASMVREITLRMTNDRDHWTSMTGKLEAQLKSLEQEEIRLRTELELVRTENTELESEQQKAHIQITELLEQNIKLNQELAQTSSSIGGTPEHSPLRPRRHSEDKEEEMLQLMEKLAALQMENAQLRDKTDELTIEIESLNVELIRSKTKAKKQEKQEKQEDQESAATATKRRGDSPSKTHLTEESPRLGKQRKCTEGEQSDASNSGDWLALNSELQRSQSQDEELTSLRQRVAELEEELKAAKEGRSLTPESRSKELETSLEQMQRAYEDCEDYWQTKLSEERQLFEKERQIYEDEQHESDKKFTELMEKVREYEEQFSKDGRLSPIDERDMLEQQYSELEAEAAQLRSSSIQMLEEKAQEISSLQSEIEDLRQRLGESVEILTGACELTSESVAQLSAEAGKSPASSPISYLWLQSTIQEPAKSLADSKDEATASAIELLGGSPSHKTASRNNLTTSETSIFSTTPFESSQSGPSPTNSGNSNAYGANPGPAPISKPKRSQSPQQAAASEGEIADCETSSTASGKSFESNSKTSCLSHEKCSSPSALKEELKRLKFFELSLKEQIKDLSLQRDGLVMELQQLQEARPVLEKAYARTTHPTLQQRLNQLELRNRHLQNVIKQQQHYTESLMQQSWRQHQVELNDLHSRIETQGVLLADQTQRLQSADILVKDLYVENSHLTATVQRLEQQRARVNLIHQQQQQQRLVGGGLPGMP

Target ORF information:

RefSeq Version	NM_164637.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164637.3

ORF Insert Sequence:

ATGGAGGTAT CCGCCGATCC GTACGAGCAG AAGCTCTACC AAATGTTCCG CAGCTGCGAG 
ACGCAGTGTG GACTTCTGGA CGAGAAGTCC CTGCTGAAGC TCTGCTCACT GCTGGAGCTC 
CGGGATCAGG GATCCGCACT GATCGCCAGC CTGGGCGGCA GCCATCAGCT GGGCGTGTCC 
TTTGGCCAGT TCAAGGAGGC GCTACTCAAC TTCCTGGGCT CCGAGTTCGA TGGTAATACG 
TCATCGGGTT TCATTGAGCG TTCACTGGTG ATTACGGATG AGCCGCTAAA CAACACATAC 
ATCGAGAGTC CGCCGGAGTC TTCCGATCGC GAGGTTTCAC CCAAACTCGT CGTGGGCACC 
AAGAAATACG GTCGCCGGTC TAGGCCACAG CAGGGAATCT ACGAGTTATC CGTCACGGAC 
TCGGACAATA CGGACGAGGA CCAGTTGCAG CAGCAGCAAA ATCAGCGAAG CCTCAACGGA 
TGCGATGAGC TGGGAGTTCA GGTGCAACGT TCCTCGTCCC AGAGCGATCT TCCTGGCAGC 
CGGCGTCTGC GGTCCGTCCA CACCAGCGGG AGCAAACTGA AGCGTTGTGC TTCACTGCCA 
GCACGCCGGA AGATGAACAG CAACACCACG GGAGCCACTA CATCACCGAC GGCAGCGGCC 
AAGTTGAAAC AGCTTTCCAT CCAGAGCCAG GCGCAGCACA GCAGCAGCGT GGAATCACTG 
GACACCGTGA CGCCGCAGCA ATTGGAGACG ATCTCAGTGC ATAGCATTAT GGAAGCCTGG 
GAGCTGGCCA GCATTCCCAA CACTCGCAAC CTACTTCACG TCCTGGGATT CGATGAGGAG 
GAGGAGGTGA ACCTGCAGCA GCTAACTAAG GCATTGGAGG AGGAGCTGCG GGGCATCGAT 
GGGGATCACG AGCAATCGAA TATGTTGCGC GCTCTGGCTG CTCTGCAGGC CACCGAGTTG 
GGCAACTACA GACTTGCCTA TAGGCAGCAG CATGAGGAGA ACCTCAAGCT GAGGGCCGAT 
AATAAGGCGG CCAACCAAAG GGTGGCTTTG CTTGCCGTGG AAGTGGATGA GCGGCATGCG 
TCGCTGGAGG ATAACTCCAA GAAGCAGGTG CAGCAGCTGG AGCAAAGACA CGCCAGCATG 
GTGCGTGAAA TAACGCTGCG GATGACTAAT GACCGCGATC ACTGGACCAG CATGACGGGA 
AAGCTGGAGG CACAGCTTAA ATCGCTTGAG CAGGAGGAGA TCCGTCTGAG AACGGAACTT 
GAACTGGTGC GCACTGAGAA CACGGAGCTT GAGTCGGAGC AGCAAAAGGC TCACATCCAA 
ATCACAGAGC TTCTCGAACA GAACATTAAG CTCAACCAGG AACTGGCCCA AACGTCGAGC 
AGCATTGGTG GCACCCCGGA GCACAGTCCA TTGCGACCGA GAAGGCATAG CGAGGACAAG 
GAGGAGGAGA TGCTCCAGCT AATGGAGAAG CTGGCTGCTC TTCAAATGGA GAACGCCCAG 
CTGCGTGACA AGACTGACGA ACTGACCATC GAAATCGAGA GCTTAAATGT GGAACTAATT 
CGCTCGAAAA CCAAGGCTAA AAAGCAAGAA AAACAGGAGA AACAAGAGGA CCAGGAGTCG 
GCGGCCACGG CTACCAAAAG GCGTGGGGAT TCGCCGAGCA AAACACATCT AACAGAGGAG 
AGCCCTCGCT TGGGGAAACA GCGCAAGTGC ACCGAAGGAG AGCAGAGCGA TGCCAGCAAC 
AGCGGAGATT GGTTGGCTCT AAACTCCGAG CTGCAAAGAA GTCAAAGCCA GGATGAGGAG 
CTAACAAGCC TTAGACAGCG GGTTGCTGAG CTAGAGGAGG AACTCAAGGC TGCAAAGGAA 
GGCAGATCTC TCACCCCGGA AAGCCGTTCG AAGGAACTGG AGACCAGTCT AGAGCAAATG 
CAGCGTGCCT ATGAGGATTG CGAGGACTAC TGGCAAACGA AACTTAGCGA GGAGCGGCAG 
CTGTTTGAGA AGGAGCGACA GATCTACGAA GATGAGCAGC ACGAGAGCGA CAAGAAGTTC 
ACCGAGCTGA TGGAAAAGGT GCGCGAGTAC GAGGAGCAGT TCAGCAAGGA TGGCCGCCTC 
TCGCCCATTG ATGAGCGCGA TATGCTGGAA CAGCAGTACT CGGAATTGGA GGCAGAGGCA 
GCCCAGCTGC GCTCGAGTTC CATTCAAATG CTCGAGGAGA AGGCTCAGGA AATCAGTTCA 
CTGCAATCAG AGATCGAGGA TTTGCGACAG AGATTGGGTG AGAGCGTTGA GATCCTCACA 
GGCGCCTGTG AACTCACCTC GGAGTCGGTA GCCCAACTGA GTGCCGAGGC GGGAAAAAGT 
CCAGCCAGCT CACCCATCAG CTACCTCTGG CTGCAGAGCA CCATCCAAGA GCCAGCGAAA 
TCGCTTGCCG ATTCCAAGGA TGAAGCCACC GCCAGTGCCA TCGAATTGCT CGGAGGCTCA 
CCATCGCACA AGACAGCCAG CCGCAATAAC CTCACCACTT CGGAAACATC CATCTTTAGC 
ACTACACCCT TCGAAAGCTC TCAGTCGGGT CCTTCGCCCA CGAACAGTGG CAACAGCAAC 
GCCTACGGCG CCAATCCCGG CCCAGCTCCG ATCAGCAAGC CCAAGCGGTC CCAGAGTCCC 
CAACAGGCGG CTGCATCGGA GGGAGAGATA GCCGATTGCG AGACGTCGTC GACGGCGTCC 
GGCAAAAGCT TCGAATCCAA CAGTAAAACG TCTTGCCTTA GCCACGAGAA GTGCAGCAGT 
CCGTCGGCAC TGAAGGAGGA ACTGAAGCGC CTTAAGTTCT TCGAGCTCTC CCTCAAGGAG 
CAAATCAAGG ATCTGAGTCT GCAGCGGGAC GGTCTGGTCA TGGAACTGCA GCAGTTGCAG 
GAGGCGCGAC CCGTGCTCGA GAAGGCCTAT GCGCGAACAA CGCATCCAAC GCTTCAGCAG 
CGACTGAACC AATTGGAGCT GCGAAATCGC CATCTGCAGA ATGTCATCAA GCAGCAGCAG 
CATTACACGG AGTCCCTGAT GCAGCAATCC TGGCGGCAGC ATCAAGTGGA GCTCAACGAT 
TTGCATAGCC GAATCGAGAC CCAGGGTGTT TTACTGGCCG ATCAGACACA GCGATTGCAG 
AGTGCCGACA TCCTGGTGAA GGATCTATAT GTGGAGAACT CCCATCTGAC GGCTACGGTG 
CAGCGGTTGG AGCAGCAACG AGCTAGGGTG AACCTCATTC ACCAGCAGCA GCAACAGCAG 
CGCCTTGTGG GCGGTGGACT GCCTGGCATG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bsg25D ( NM_164638.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723073.1 Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant C, mRNA.

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CloneID	OFa16261
Clone ID Related Accession (Same CDS sequence)	NM_164638.3
Accession Version	NM_164638.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blastoderm-specific gene 25D, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164638.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAACAC GATTGTATTA CCGCCGATCC GGCGGTCAGT TAACAGATCC GATAATTGAG 
AAGCTAGCCG CTCGTTTTGA GCGTTCACTG GTGATTACGG ATGAGCCGCT AAACAACACA 
TACATCGAGA GTCCGCCGGA GTCTTCCGAT CGCGAGGTTT CACCCAAACT CGTCGTGGGC 
ACCAAGAAAT ACGGTCGCCG GTCTAGGCCA CAGCAGGGAA TCTACGAGTT ATCCGTCACG 
GACTCGGACA ATACGGACGA GGACCAGTTG CAGCAGCAGC AAAATCAGCG AAGCCTCAAC 
GGATGCGATG AGCTGGGAGT TCAGGTGCAA CGTTCCTCGT CCCAGAGCGA TCTTCCTGGC 
AGCCGGCGTC TGCGGTCCGT CCACACCAGC GGGAGCAAAC TGAAGCGTTG TGCTTCACTG 
CCAGCACGCC GGAAGATGAA CAGCAACACC ACGGGAGCCA CTACATCACC GACGGCAGCG 
GCCAAGTTGA AACAGCTTTC CATCCAGAGC CAGGCGCAGC ACAGCAGCAG CGTGGAATCA 
CTGGACACCG TGACGCCGCA GCAATTGGAG ACGATCTCAG TGCATAGCAT TATGGAAGCC 
TGGGAGCTGG CCAGCATTCC CAACACTCGC AACCTACTTC ACGTCCTGGG ATTCGATGAG 
GAGGAGGAGG TGAACCTGCA GCAGCTAACT AAGGCATTGG AGGAGGAGCT GCGGGGCATC 
GATGGGGATC ACGAGCAATC GAATATGTTG CGCGCTCTGG CTGCTCTGCA GGCCACCGAG 
TTGGGCAACT ACAGACTTGC CTATAGGCAG CAGCATGAGG AGAACCTCAA GCTGAGGGCC 
GATAATAAGG CGGCCAACCA AAGGGTGGCT TTGCTTGCCG TGGAAGTGGA TGAGCGGCAT 
GCGTCGCTGG AGGATAACTC CAAGAAGCAG GTGCAGCAGC TGGAGCAAAG ACACGCCAGC 
ATGGTGCGTG AAATAACGCT GCGGATGACT AATGACCGCG ATCACTGGAC CAGCATGACG 
GGAAAGCTGG AGGCACAGCT TAAATCGCTT GAGCAGGAGG AGATCCGTCT GAGAACGGAA 
CTTGAACTGG TGCGCACTGA GAACACGGAG CTTGAGTCGG AGCAGCAAAA GGCTCACATC 
CAAATCACAG AGCTTCTCGA ACAGAACATT AAGCTCAACC AGGAACTGGC CCAAACGTCG 
AGCAGCATTG GTGGCACCCC GGAGCACAGT CCATTGCGAC CGAGAAGGCA TAGCGAGGAC 
AAGGAGGAGG AGATGCTCCA GCTAATGGAG AAGCTGGCTG CTCTTCAAAT GGAGAACGCC 
CAGCTGCGTG ACAAGACTGA CGAACTGACC ATCGAAATCG AGAGCTTAAA TGTGGAACTA 
ATTCGCTCGA AAACCAAGGC TAAAAAGCAA GAAAAACAGG AGAAACAAGA GGACCAGGAG 
TCGGCGGCCA CGGCTACCAA AAGGCGTGGG GATTCGCCGA GCAAAACACA TCTAACAGAG 
GAGAGCCCTC GCTTGGGGAA ACAGCGCAAG TGCACCGAAG GAGAGCAGAG CGATGCCAGC 
AACAGCGGAG ATTGGTTGGC TCTAAACTCC GAGCTGCAAA GAAGTCAAAG CCAGGATGAG 
GAGCTAACAA GCCTTAGACA GCGGGTTGCT GAGCTAGAGG AGGAACTCAA GGCTGCAAAG 
GAAGGCAGAT CTCTCACCCC GGAAAGCCGT TCGAAGGAAC TGGAGACCAG TCTAGAGCAA 
ATGCAGCGTG CCTATGAGGA TTGCGAGGAC TACTGGCAAA CGAAACTTAG CGAGGAGCGG 
CAGCTGTTTG AGAAGGAGCG ACAGATCTAC GAAGATGAGC AGCACGAGAG CGACAAGAAG 
TTCACCGAGC TGATGGAAAA GGTGCGCGAG TACGAGGAGC AGTTCAGCAA GGATGGCCGC 
CTCTCGCCCA TTGATGAGCG CGATATGCTG GAACAGCAGT ACTCGGAATT GGAGGCAGAG 
GCAGCCCAGC TGCGCTCGAG TTCCATTCAA ATGCTCGAGG AGAAGGCTCA GGAAATCAGT 
TCACTGCAAT CAGAGATCGA GGATTTGCGA CAGAGATTGG GTGAGAGCGT TGAGATCCTC 
ACAGGCGCCT GTGAACTCAC CTCGGAGTCG GTAGCCCAAC TGAGTGCCGA GGCGGGAAAA 
AGTCCAGCCA GCTCACCCAT CAGCTACCTC TGGCTGCAGA GCACCATCCA AGAGCCAGCG 
AAATCGCTTG CCGATTCCAA GGATGAAGCC ACCGCCAGTG CCATCGAATT GCTCGGAGGC 
TCACCATCGC ACAAGACAGC CAGCCGCAAT AACCTCACCA CTTCGGAAAC ATCCATCTTT 
AGCACTACAC CCTTCGAAAG CTCTCAGTCG GGTCCTTCGC CCACGAACAG TGGCAACAGC 
AACGCCTACG GCGCCAATCC CGGCCCAGCT CCGATCAGCA AGCCCAAGCG GTCCCAGAGT 
CCCCAACAGG CGGCTGCATC GGAGGGAGAG ATAGCCGATT GCGAGACGTC GTCGACGGCG 
TCCGGCAAAA GCTTCGAATC CAACAGTAAA ACGTCTTGCC TTAGCCACGA GAAGTGCAGC 
AGTCCGTCGG CACTGAAGGA GGAACTGAAG CGCCTTAAGT TCTTCGAGCT CTCCCTCAAG 
GAGCAAATCA AGGATCTGAG TCTGCAGCGG GACGGTCTGG TCATGGAACT GCAGCAGTTG 
CAGGAGGCGC GACCCGTGCT CGAGAAGGCC TATGCGCGAA CAACGCATCC AACGCTTCAG 
CAGCGACTGA ACCAATTGGA GCTGCGAAAT CGCCATCTGC AGAATGTCAT CAAGCAGCAG 
CAGCATTACA CGGAGTCCCT GATGCAGCAA TCCTGGCGGC AGCATCAAGT GGAGCTCAAC 
GATTTGCATA GCCGAATCGA GACCCAGGGT GTTTTACTGG CCGATCAGAC ACAGCGATTG 
CAGAGTGCCG ACATCCTGGT GAAGGATCTA TATGTGGAGA ACTCCCATCT GACGGCTACG 
GTGCAGCGGT TGGAGCAGCA ACGAGCTAGG GTGAACCTCA TTCACCAGCA GCAGCAACAG 
CAGCGCCTTG TGGGCGGTGG ACTGCCTGGC ATGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723073.1
CDS	17..3115
Translation	MGTRLYYRRSGGQLTDPIIEKLAARFERSLVITDEPLNNTYIESPPESSDREVSPKLVVGTKKYGRRSRPQQGIYELSVTDSDNTDEDQLQQQQNQRSLNGCDELGVQVQRSSSQSDLPGSRRLRSVHTSGSKLKRCASLPARRKMNSNTTGATTSPTAAAKLKQLSIQSQAQHSSSVESLDTVTPQQLETISVHSIMEAWELASIPNTRNLLHVLGFDEEEEVNLQQLTKALEEELRGIDGDHEQSNMLRALAALQATELGNYRLAYRQQHEENLKLRADNKAANQRVALLAVEVDERHASLEDNSKKQVQQLEQRHASMVREITLRMTNDRDHWTSMTGKLEAQLKSLEQEEIRLRTELELVRTENTELESEQQKAHIQITELLEQNIKLNQELAQTSSSIGGTPEHSPLRPRRHSEDKEEEMLQLMEKLAALQMENAQLRDKTDELTIEIESLNVELIRSKTKAKKQEKQEKQEDQESAATATKRRGDSPSKTHLTEESPRLGKQRKCTEGEQSDASNSGDWLALNSELQRSQSQDEELTSLRQRVAELEEELKAAKEGRSLTPESRSKELETSLEQMQRAYEDCEDYWQTKLSEERQLFEKERQIYEDEQHESDKKFTELMEKVREYEEQFSKDGRLSPIDERDMLEQQYSELEAEAAQLRSSSIQMLEEKAQEISSLQSEIEDLRQRLGESVEILTGACELTSESVAQLSAEAGKSPASSPISYLWLQSTIQEPAKSLADSKDEATASAIELLGGSPSHKTASRNNLTTSETSIFSTTPFESSQSGPSPTNSGNSNAYGANPGPAPISKPKRSQSPQQAAASEGEIADCETSSTASGKSFESNSKTSCLSHEKCSSPSALKEELKRLKFFELSLKEQIKDLSLQRDGLVMELQQLQEARPVLEKAYARTTHPTLQQRLNQLELRNRHLQNVIKQQQHYTESLMQQSWRQHQVELNDLHSRIETQGVLLADQTQRLQSADILVKDLYVENSHLTATVQRLEQQRARVNLIHQQQQQQRLVGGGLPGMP

Target ORF information:

RefSeq Version	NM_164638.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blastoderm-specific gene 25D (Bsg25D), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164638.3

ORF Insert Sequence:

ATGGGAACAC GATTGTATTA CCGCCGATCC GGCGGTCAGT TAACAGATCC GATAATTGAG 
AAGCTAGCCG CTCGTTTTGA GCGTTCACTG GTGATTACGG ATGAGCCGCT AAACAACACA 
TACATCGAGA GTCCGCCGGA GTCTTCCGAT CGCGAGGTTT CACCCAAACT CGTCGTGGGC 
ACCAAGAAAT ACGGTCGCCG GTCTAGGCCA CAGCAGGGAA TCTACGAGTT ATCCGTCACG 
GACTCGGACA ATACGGACGA GGACCAGTTG CAGCAGCAGC AAAATCAGCG AAGCCTCAAC 
GGATGCGATG AGCTGGGAGT TCAGGTGCAA CGTTCCTCGT CCCAGAGCGA TCTTCCTGGC 
AGCCGGCGTC TGCGGTCCGT CCACACCAGC GGGAGCAAAC TGAAGCGTTG TGCTTCACTG 
CCAGCACGCC GGAAGATGAA CAGCAACACC ACGGGAGCCA CTACATCACC GACGGCAGCG 
GCCAAGTTGA AACAGCTTTC CATCCAGAGC CAGGCGCAGC ACAGCAGCAG CGTGGAATCA 
CTGGACACCG TGACGCCGCA GCAATTGGAG ACGATCTCAG TGCATAGCAT TATGGAAGCC 
TGGGAGCTGG CCAGCATTCC CAACACTCGC AACCTACTTC ACGTCCTGGG ATTCGATGAG 
GAGGAGGAGG TGAACCTGCA GCAGCTAACT AAGGCATTGG AGGAGGAGCT GCGGGGCATC 
GATGGGGATC ACGAGCAATC GAATATGTTG CGCGCTCTGG CTGCTCTGCA GGCCACCGAG 
TTGGGCAACT ACAGACTTGC CTATAGGCAG CAGCATGAGG AGAACCTCAA GCTGAGGGCC 
GATAATAAGG CGGCCAACCA AAGGGTGGCT TTGCTTGCCG TGGAAGTGGA TGAGCGGCAT 
GCGTCGCTGG AGGATAACTC CAAGAAGCAG GTGCAGCAGC TGGAGCAAAG ACACGCCAGC 
ATGGTGCGTG AAATAACGCT GCGGATGACT AATGACCGCG ATCACTGGAC CAGCATGACG 
GGAAAGCTGG AGGCACAGCT TAAATCGCTT GAGCAGGAGG AGATCCGTCT GAGAACGGAA 
CTTGAACTGG TGCGCACTGA GAACACGGAG CTTGAGTCGG AGCAGCAAAA GGCTCACATC 
CAAATCACAG AGCTTCTCGA ACAGAACATT AAGCTCAACC AGGAACTGGC CCAAACGTCG 
AGCAGCATTG GTGGCACCCC GGAGCACAGT CCATTGCGAC CGAGAAGGCA TAGCGAGGAC 
AAGGAGGAGG AGATGCTCCA GCTAATGGAG AAGCTGGCTG CTCTTCAAAT GGAGAACGCC 
CAGCTGCGTG ACAAGACTGA CGAACTGACC ATCGAAATCG AGAGCTTAAA TGTGGAACTA 
ATTCGCTCGA AAACCAAGGC TAAAAAGCAA GAAAAACAGG AGAAACAAGA GGACCAGGAG 
TCGGCGGCCA CGGCTACCAA AAGGCGTGGG GATTCGCCGA GCAAAACACA TCTAACAGAG 
GAGAGCCCTC GCTTGGGGAA ACAGCGCAAG TGCACCGAAG GAGAGCAGAG CGATGCCAGC 
AACAGCGGAG ATTGGTTGGC TCTAAACTCC GAGCTGCAAA GAAGTCAAAG CCAGGATGAG 
GAGCTAACAA GCCTTAGACA GCGGGTTGCT GAGCTAGAGG AGGAACTCAA GGCTGCAAAG 
GAAGGCAGAT CTCTCACCCC GGAAAGCCGT TCGAAGGAAC TGGAGACCAG TCTAGAGCAA 
ATGCAGCGTG CCTATGAGGA TTGCGAGGAC TACTGGCAAA CGAAACTTAG CGAGGAGCGG 
CAGCTGTTTG AGAAGGAGCG ACAGATCTAC GAAGATGAGC AGCACGAGAG CGACAAGAAG 
TTCACCGAGC TGATGGAAAA GGTGCGCGAG TACGAGGAGC AGTTCAGCAA GGATGGCCGC 
CTCTCGCCCA TTGATGAGCG CGATATGCTG GAACAGCAGT ACTCGGAATT GGAGGCAGAG 
GCAGCCCAGC TGCGCTCGAG TTCCATTCAA ATGCTCGAGG AGAAGGCTCA GGAAATCAGT 
TCACTGCAAT CAGAGATCGA GGATTTGCGA CAGAGATTGG GTGAGAGCGT TGAGATCCTC 
ACAGGCGCCT GTGAACTCAC CTCGGAGTCG GTAGCCCAAC TGAGTGCCGA GGCGGGAAAA 
AGTCCAGCCA GCTCACCCAT CAGCTACCTC TGGCTGCAGA GCACCATCCA AGAGCCAGCG 
AAATCGCTTG CCGATTCCAA GGATGAAGCC ACCGCCAGTG CCATCGAATT GCTCGGAGGC 
TCACCATCGC ACAAGACAGC CAGCCGCAAT AACCTCACCA CTTCGGAAAC ATCCATCTTT 
AGCACTACAC CCTTCGAAAG CTCTCAGTCG GGTCCTTCGC CCACGAACAG TGGCAACAGC 
AACGCCTACG GCGCCAATCC CGGCCCAGCT CCGATCAGCA AGCCCAAGCG GTCCCAGAGT 
CCCCAACAGG CGGCTGCATC GGAGGGAGAG ATAGCCGATT GCGAGACGTC GTCGACGGCG 
TCCGGCAAAA GCTTCGAATC CAACAGTAAA ACGTCTTGCC TTAGCCACGA GAAGTGCAGC 
AGTCCGTCGG CACTGAAGGA GGAACTGAAG CGCCTTAAGT TCTTCGAGCT CTCCCTCAAG 
GAGCAAATCA AGGATCTGAG TCTGCAGCGG GACGGTCTGG TCATGGAACT GCAGCAGTTG 
CAGGAGGCGC GACCCGTGCT CGAGAAGGCC TATGCGCGAA CAACGCATCC AACGCTTCAG 
CAGCGACTGA ACCAATTGGA GCTGCGAAAT CGCCATCTGC AGAATGTCAT CAAGCAGCAG 
CAGCATTACA CGGAGTCCCT GATGCAGCAA TCCTGGCGGC AGCATCAAGT GGAGCTCAAC 
GATTTGCATA GCCGAATCGA GACCCAGGGT GTTTTACTGG CCGATCAGAC ACAGCGATTG 
CAGAGTGCCG ACATCCTGGT GAAGGATCTA TATGTGGAGA ACTCCCATCT GACGGCTACG 
GTGCAGCGGT TGGAGCAGCA ACGAGCTAGG GTGAACCTCA TTCACCAGCA GCAGCAACAG 
CAGCGCCTTG TGGGCGGTGG ACTGCCTGGC ATGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Bsg25D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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