Shaw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shaw
Entrez Gene ID	33599
Full Name	Shaker cognate w
Synonyms	CG15419,CG2822,Dmel\CG2822,K[[v]]3,Kv3,Kv3.1,Shaw2,dShaw,dShaw2,shaw
General protein information	Preferred Names Shaker cognate w Names CG2822 gene product from transcript CG2822-RE CG2822-PA CG2822-PB CG2822-PC CG2822-PD CG2822-PE Shaw-PA Shaw-PB Shaw-PC Shaw-PD Shaw-PE shaker cognate w
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24C1-24C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057373.3	NP_476721.1	shaker cognate w, isoform A
NM_164554.3	NP_722937.2	shaker cognate w, isoform B
NM_001273071.1	NP_001260000.1	shaker cognate w, isoform D
NM_001144310.1	NP_001137782.1	shaker cognate w, isoform C
NM_001273072.1	NP_001260001.1	shaker cognate w, isoform E

Pathways from BioSystems

Homologies

	KCNC2	XP_002798726.1
Mus musculus (house mouse)	Kcnc2	NP_001020752.1
Rattus norvegicus (Norway rat)	Kcnc2	NP_631963.1
Caenorhabditis elegans (roundworm)	shw-1	NP_001022089.1
Xenopus tropicalis (tropical clawed frog)	kcnc2	XP_002939991.2
Homo sapiens (human)	KCNC2	NP_631875.1
Gallus gallus (chicken)	KCNC2	XP_001235255.3
Pan troglodytes (chimpanzee)	KCNC2	XP_003313895.2
Canis lupus familiaris (dog)	KCNC2	XP_005625689.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008200	XP_317268.4
Danio rerio (zebrafish)	kcnc2	NP_001082998.2
Drosophila melanogaster (fruit fly)	Shaw	NP_476721.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

increasing Shaw levels in all clock neurons (LNv, LNd, DN(1), DN(2) and DN(3)), or in subsets of clock neurons (LNd and DNs or DNs alone) increases locomotor activity at night
Title: Function of the Shaw potassium channel within the Drosophila circadian clock.

The following Shaw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shaw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13677	NM_057373.3 Latest version!	Drosophila melanogaster shaker cognate w, transcript variant A (Shaw), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13678	NM_164554.3 Latest version!	Drosophila melanogaster shaker cognate w, transcript variant B (Shaw), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13679	NM_001144310.1 Latest version!	Drosophila melanogaster shaker cognate w, transcript variant C (Shaw), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16163	NM_001273072.1 Latest version!	Drosophila melanogaster shaker cognate w, transcript variant E (Shaw), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16164	NM_001273071.1 Latest version!	Drosophila melanogaster shaker cognate w, transcript variant D (Shaw), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shaw ( NM_057373.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476721.1 Drosophila melanogaster shaker cognate w, transcript variant A (Shaw), mRNA.

Price & Availability↑

CloneID	OFa13677
Clone ID Related Accession (Same CDS sequence)	NM_057373.3
Accession Version	NM_057373.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaker cognate w, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057373.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTGA TCAACATGGA CTCGGAAAAC AGGGTGGTGC TCAATGTGGG TGGCATTAGG 
CACGAAACCT ACAAGGCCAC GCTGAAGAAG ATTCCGGCTA CGCGATTATC GCGATTAACA 
GAGGCGCTGG CCAACTATGA TCCGATACTG AATGAGTACT TCTTTGATCG GCATCCGGGC 
GTCTTCGCAC AAGTGCTCAA CTATTACAGA ACTGGAAAGC TGCATTATCC CACGGATGTG 
TGCGGTCCGC TGTTTGAGGA GGAATTGGAG TTCTGGGGCC TAGACTCGAA CCAAGTGGAG 
CCCTGCTGTT GGATGACCTA CACACAGCAT CGCGACACCC AGGAAACCCT AGCCGTACTC 
GATCGTCTCG ATCTGGATAC GGAAAAACCG TCCGAAGAGG AATTGGCACG CAAATTCGGC 
TTCGAGGAGG ACTACTACAA AGGCACAATA TCCTGGTGGC AGGAAATGAA GCCGCGCATT 
TGGTCCTTGT TCGATGAGCC CTACAGTTCC AATGCAGCCA AGACTATTGG CGTGGTTTCG 
GTGTTCTTCA TCTGCATTTC GATCCTGTCG TTCTGCCTGA AGACCCATCC CGATATGCGG 
GTACCCATCG TCCGGAACAT TACAGTGAAA ACTGCGAATG GAAGTAATGG CTGGTTTTTG 
GACAAAACGC AGACCAATGC GCACATAGCC TTCTTCTATA TCGAATGCGT GTGCAATGCC 
TGGTTTACCT TTGAAATATT GGTGCGCTTT ATCTCATCGC CGAACAAGTG GGAGTTCATC 
AAGTCATCTG TTAACATCAT AGACTACATA GCGACGCTTA GTTTTTATAT CGATCTAGTG 
CTTCAGCGGT TCGCATCGCA CCTGGAGAAC GCTGACATCC TCGAGTTCTT CTCGATCATC 
CGCATCATGC GTCTGTTCAA GCTGACGCGC CACTCGTCCG GACTGAAGAT CCTGATCCAG 
ACGTTCCGGG CCTCGGCCAA GGAGCTGACC CTGCTGGTGT TCTTCCTCGT CCTGGGCATC 
GTGATCTTCG CCAGCCTTGT CTACTACGCG GAGCGCATCC AGCCCAATCC GCACAACGAC 
TTCAACAGCA TACCGCTGGG CCTGTGGTGG GCCCTGGTCA CAATGACCAC CGTCGGCTAC 
GGCGACATGG CCCCCAAAAC CTACATCGGC ATGTTCGTGG GCGCCCTCTG CGCCCTGGCC 
GGCGTACTAA CCATCGCACT GCCAGTGCCC GTCATCGTCA GCAACTTCGC CATGTACTAC 
TCGCACACGC AGGCCAGGGC CAAACTGCCA AAGAAGCGGA GACGAGTGCT TCCCGTCGAG 
CAGCCGCGCC AGCCCAGACT GCCAGGTGCC CCTGGTGGTG TCAGTGGTTG CGGTACCCCG 
GGCTCGGGTC CCCACTCCGG TCCCATGGGA TCCGGCGGTA CTGGACCACG TCGCATGAAC 
AATAAAACAA AGGACCTGGT CAGCCCCAAG TCAGATATGG CCTTCAGTTT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476721.1
CDS	458..1954
Translation	MNLINMDSENRVVLNVGGIRHETYKATLKKIPATRLSRLTEALANYDPILNEYFFDRHPGVFAQVLNYYRTGKLHYPTDVCGPLFEEELEFWGLDSNQVEPCCWMTYTQHRDTQETLAVLDRLDLDTEKPSEEELARKFGFEEDYYKGTISWWQEMKPRIWSLFDEPYSSNAAKTIGVVSVFFICISILSFCLKTHPDMRVPIVRNITVKTANGSNGWFLDKTQTNAHIAFFYIECVCNAWFTFEILVRFISSPNKWEFIKSSVNIIDYIATLSFYIDLVLQRFASHLENADILEFFSIIRIMRLFKLTRHSSGLKILIQTFRASAKELTLLVFFLVLGIVIFASLVYYAERIQPNPHNDFNSIPLGLWWALVTMTTVGYGDMAPKTYIGMFVGALCALAGVLTIALPVPVIVSNFAMYYSHTQARAKLPKKRRRVLPVEQPRQPRLPGAPGGVSGCGTPGSGPHSGPMGSGGTGPRRMNNKTKDLVSPKSDMAFSFD

Target ORF information:

RefSeq Version	NM_057373.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaker cognate w, transcript variant A (Shaw), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057373.3

ORF Insert Sequence:

ATGAATCTGA TCAACATGGA CTCGGAAAAC AGGGTGGTGC TCAATGTGGG TGGCATTAGG 
CACGAAACCT ACAAGGCCAC GCTGAAGAAG ATTCCGGCTA CGCGATTATC GCGATTAACA 
GAGGCGCTGG CCAACTATGA TCCGATACTG AATGAGTACT TCTTTGATCG GCATCCGGGC 
GTCTTCGCAC AAGTGCTCAA CTATTACAGA ACTGGAAAGC TGCATTATCC CACGGATGTG 
TGCGGTCCGC TGTTTGAGGA GGAATTGGAG TTCTGGGGCC TAGACTCGAA CCAAGTGGAG 
CCCTGCTGTT GGATGACCTA CACACAGCAT CGCGACACCC AGGAAACCCT AGCCGTACTC 
GATCGTCTCG ATCTGGATAC GGAAAAACCG TCCGAAGAGG AATTGGCACG CAAATTCGGC 
TTCGAGGAGG ACTACTACAA AGGCACAATA TCCTGGTGGC AGGAAATGAA GCCGCGCATT 
TGGTCCTTGT TCGATGAGCC CTACAGTTCC AATGCAGCCA AGACTATTGG CGTGGTTTCG 
GTGTTCTTCA TCTGCATTTC GATCCTGTCG TTCTGCCTGA AGACCCATCC CGATATGCGG 
GTACCCATCG TCCGGAACAT TACAGTGAAA ACTGCGAATG GAAGTAATGG CTGGTTTTTG 
GACAAAACGC AGACCAATGC GCACATAGCC TTCTTCTATA TCGAATGCGT GTGCAATGCC 
TGGTTTACCT TTGAAATATT GGTGCGCTTT ATCTCATCGC CGAACAAGTG GGAGTTCATC 
AAGTCATCTG TTAACATCAT AGACTACATA GCGACGCTTA GTTTTTATAT CGATCTAGTG 
CTTCAGCGGT TCGCATCGCA CCTGGAGAAC GCTGACATCC TCGAGTTCTT CTCGATCATC 
CGCATCATGC GTCTGTTCAA GCTGACGCGC CACTCGTCCG GACTGAAGAT CCTGATCCAG 
ACGTTCCGGG CCTCGGCCAA GGAGCTGACC CTGCTGGTGT TCTTCCTCGT CCTGGGCATC 
GTGATCTTCG CCAGCCTTGT CTACTACGCG GAGCGCATCC AGCCCAATCC GCACAACGAC 
TTCAACAGCA TACCGCTGGG CCTGTGGTGG GCCCTGGTCA CAATGACCAC CGTCGGCTAC 
GGCGACATGG CCCCCAAAAC CTACATCGGC ATGTTCGTGG GCGCCCTCTG CGCCCTGGCC 
GGCGTACTAA CCATCGCACT GCCAGTGCCC GTCATCGTCA GCAACTTCGC CATGTACTAC 
TCGCACACGC AGGCCAGGGC CAAACTGCCA AAGAAGCGGA GACGAGTGCT TCCCGTCGAG 
CAGCCGCGCC AGCCCAGACT GCCAGGTGCC CCTGGTGGTG TCAGTGGTTG CGGTACCCCG 
GGCTCGGGTC CCCACTCCGG TCCCATGGGA TCCGGCGGTA CTGGACCACG TCGCATGAAC 
AATAAAACAA AGGACCTGGT CAGCCCCAAG TCAGATATGG CCTTCAGTTT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shaw ( NM_164554.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722937.2 Drosophila melanogaster shaker cognate w, transcript variant B (Shaw), mRNA.

Price & Availability↑

CloneID	OFa13678
Clone ID Related Accession (Same CDS sequence)	NM_164554.3
Accession Version	NM_164554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaker cognate w, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164554.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTGA TCAACATGGA CTCGGAAAAC AGGGTGGTGC TCAATGTGGG TGGCATTAGG 
CACGAAACCT ACAAGGCCAC GCTGAAGAAG ATTCCGGCTA CGCGATTATC GCGATTAACA 
GAGGCGCTGG CCAACTATGA TCCGATACTG AATGAGTACT TCTTTGATCG GCATCCGGGC 
GTCTTCGCAC AAGTGCTCAA CTATTACAGA ACTGGAAAGC TGCATTATCC CACGGATGTG 
TGCGGTCCGC TGTTTGAGGA GGAATTGGAG TTCTGGGGCC TAGACTCGAA CCAAGTGGAG 
CCCTGCTGTT GGATGACCTA CACACAGCAT CGCGACACCC AGGAAACCCT AGCCGTACTC 
GATCGTCTCG ATCTGGATAC GGAAAAACCG TCCGAAGAGG AATTGGCACG CAAATTCGGC 
TTCGAGGAGG ACTACTACAA AGGCACAATA TCCTGGTGGC AGGAAATGAA GCCGCGCATT 
TGGTCCTTGT TCGATGAGCC CTACAGTTCC AATGCAGCCA AGACTATTGG CGTGGTTTCG 
GTGTTCTTCA TCTGCATTTC GATCCTGTCG TTCTGCCTGA AGACCCATCC CGATATGCGG 
GTACCCATCG TCCGGAACAT TACAGTGAAA ACTGCGAATG GAAGTAATGG CTGGTTTTTG 
GACAAAACGC AGACCAATGC GCACATAGCC TTCTTCTATA TCGAATGCGT GTGCAATGCC 
TGGTTTACCT TTGAAATATT GGTGCGCTTT ATCTCATCGC CGAACAAGTG GGAGTTCATC 
AAGTCATCTG TTAACATCAT AGACTACATA GCGACGCTTA GTTTTTATAT CGATCTAGTG 
CTTCAGCGGT TCGCATCGCA CCTGGAGAAC GCTGACATCC TCGAGTTCTT CTCGATCATC 
CGCATCATGC GTCTGTTCAA GCTGACGCGC CACTCGTCCG GACTGAAGAT CCTGATCCAG 
ACGTTCCGGG CCTCGGCCAA GGAGCTGACC CTGCTGGTGT TCTTCCTCGT CCTGGGCATC 
GTGATCTTCG CCAGCCTTGT CTACTACGCG GAGCGCATCC AGCCCAATCC GCACAACGAC 
TTCAACAGCA TACCGCTGGG CCTGTGGTGG GCCCTGGTCA CAATGACCAC CGTCGGCTAC 
GGCGACATGG CCCCCAAAAC CTACATCGGC ATGTTCGTGG GCGCCCTCTG CGCCCTGGCC 
GGCGTACTAA CCATCGCACT GCCAGTGCCC GTCATCGTCA GCAACTTCGC CATGTACTAC 
TCGCACACGC AGGCCAGGGC CAAACTGCCA AAGAAGCGGA GACGAGTGCT TCCCGTCGAG 
CAGCCGCGCC AGCCCAGACT GCCAGGTGCC CCTGGTGGTG TCAGTGGTTG CGGTACCCCG 
GGCTCGGGTC CCCACTCCGG TCCCATGGGA TCCGGCGGTA CTGGACCACG TCGCATGAAC 
AATAAAACAA AGGACCTGGT CAGCCCCAAG TCAGTTGCTC AACTTTTCGC AGGTCCGCTG 
GGCGCAAGCA TTGTGGCCAT GAGTCCCCGG ACAATGCTGG ATCTCAATCC TGCCCTGGCG 
ATGGGCAAGC CTACGTTTCA GCCCCGTATA CCCACCCCCC TGGCAGCCAC GCCCCCTCCC 
CCCGTGAGTT CCGCTGGGGG GATGACAGCG TCTGGAATTG GAACTACTTC AGCCACTGGA 
GCCACTAGTG CACCACAACC AGCAACACCA CTACCCAGCA TTGCGGTGTC CACCACAGCG 
AGTGTGGGCA AGGATTTGGG CATCTCGACC ACCACCACCA CGGCCCAGGA GACCAGCAAG 
AAGGCCTTCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722937.2
CDS	458..2272
Translation	MNLINMDSENRVVLNVGGIRHETYKATLKKIPATRLSRLTEALANYDPILNEYFFDRHPGVFAQVLNYYRTGKLHYPTDVCGPLFEEELEFWGLDSNQVEPCCWMTYTQHRDTQETLAVLDRLDLDTEKPSEEELARKFGFEEDYYKGTISWWQEMKPRIWSLFDEPYSSNAAKTIGVVSVFFICISILSFCLKTHPDMRVPIVRNITVKTANGSNGWFLDKTQTNAHIAFFYIECVCNAWFTFEILVRFISSPNKWEFIKSSVNIIDYIATLSFYIDLVLQRFASHLENADILEFFSIIRIMRLFKLTRHSSGLKILIQTFRASAKELTLLVFFLVLGIVIFASLVYYAERIQPNPHNDFNSIPLGLWWALVTMTTVGYGDMAPKTYIGMFVGALCALAGVLTIALPVPVIVSNFAMYYSHTQARAKLPKKRRRVLPVEQPRQPRLPGAPGGVSGCGTPGSGPHSGPMGSGGTGPRRMNNKTKDLVSPKSVAQLFAGPLGASIVAMSPRTMLDLNPALAMGKPTFQPRIPTPLAATPPPPVSSAGGMTASGIGTTSATGATSAPQPATPLPSIAVSTTASVGKDLGISTTTTTAQETSKKAFL

Target ORF information:

RefSeq Version	NM_164554.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaker cognate w, transcript variant B (Shaw), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164554.3

ORF Insert Sequence:

ATGAATCTGA TCAACATGGA CTCGGAAAAC AGGGTGGTGC TCAATGTGGG TGGCATTAGG 
CACGAAACCT ACAAGGCCAC GCTGAAGAAG ATTCCGGCTA CGCGATTATC GCGATTAACA 
GAGGCGCTGG CCAACTATGA TCCGATACTG AATGAGTACT TCTTTGATCG GCATCCGGGC 
GTCTTCGCAC AAGTGCTCAA CTATTACAGA ACTGGAAAGC TGCATTATCC CACGGATGTG 
TGCGGTCCGC TGTTTGAGGA GGAATTGGAG TTCTGGGGCC TAGACTCGAA CCAAGTGGAG 
CCCTGCTGTT GGATGACCTA CACACAGCAT CGCGACACCC AGGAAACCCT AGCCGTACTC 
GATCGTCTCG ATCTGGATAC GGAAAAACCG TCCGAAGAGG AATTGGCACG CAAATTCGGC 
TTCGAGGAGG ACTACTACAA AGGCACAATA TCCTGGTGGC AGGAAATGAA GCCGCGCATT 
TGGTCCTTGT TCGATGAGCC CTACAGTTCC AATGCAGCCA AGACTATTGG CGTGGTTTCG 
GTGTTCTTCA TCTGCATTTC GATCCTGTCG TTCTGCCTGA AGACCCATCC CGATATGCGG 
GTACCCATCG TCCGGAACAT TACAGTGAAA ACTGCGAATG GAAGTAATGG CTGGTTTTTG 
GACAAAACGC AGACCAATGC GCACATAGCC TTCTTCTATA TCGAATGCGT GTGCAATGCC 
TGGTTTACCT TTGAAATATT GGTGCGCTTT ATCTCATCGC CGAACAAGTG GGAGTTCATC 
AAGTCATCTG TTAACATCAT AGACTACATA GCGACGCTTA GTTTTTATAT CGATCTAGTG 
CTTCAGCGGT TCGCATCGCA CCTGGAGAAC GCTGACATCC TCGAGTTCTT CTCGATCATC 
CGCATCATGC GTCTGTTCAA GCTGACGCGC CACTCGTCCG GACTGAAGAT CCTGATCCAG 
ACGTTCCGGG CCTCGGCCAA GGAGCTGACC CTGCTGGTGT TCTTCCTCGT CCTGGGCATC 
GTGATCTTCG CCAGCCTTGT CTACTACGCG GAGCGCATCC AGCCCAATCC GCACAACGAC 
TTCAACAGCA TACCGCTGGG CCTGTGGTGG GCCCTGGTCA CAATGACCAC CGTCGGCTAC 
GGCGACATGG CCCCCAAAAC CTACATCGGC ATGTTCGTGG GCGCCCTCTG CGCCCTGGCC 
GGCGTACTAA CCATCGCACT GCCAGTGCCC GTCATCGTCA GCAACTTCGC CATGTACTAC 
TCGCACACGC AGGCCAGGGC CAAACTGCCA AAGAAGCGGA GACGAGTGCT TCCCGTCGAG 
CAGCCGCGCC AGCCCAGACT GCCAGGTGCC CCTGGTGGTG TCAGTGGTTG CGGTACCCCG 
GGCTCGGGTC CCCACTCCGG TCCCATGGGA TCCGGCGGTA CTGGACCACG TCGCATGAAC 
AATAAAACAA AGGACCTGGT CAGCCCCAAG TCAGTTGCTC AACTTTTCGC AGGTCCGCTG 
GGCGCAAGCA TTGTGGCCAT GAGTCCCCGG ACAATGCTGG ATCTCAATCC TGCCCTGGCG 
ATGGGCAAGC CTACGTTTCA GCCCCGTATA CCCACCCCCC TGGCAGCCAC GCCCCCTCCC 
CCCGTGAGTT CCGCTGGGGG GATGACAGCG TCTGGAATTG GAACTACTTC AGCCACTGGA 
GCCACTAGTG CACCACAACC AGCAACACCA CTACCCAGCA TTGCGGTGTC CACCACAGCG 
AGTGTGGGCA AGGATTTGGG CATCTCGACC ACCACCACCA CGGCCCAGGA GACCAGCAAG 
AAGGCCTTCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shaw ( NM_001144310.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137782.1 Drosophila melanogaster shaker cognate w, transcript variant C (Shaw), partial mRNA.

Price & Availability↑

CloneID	OFa13679
Clone ID Related Accession (Same CDS sequence)	NM_001144310.1
Accession Version	NM_001144310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaker cognate w, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGACTTATA TACCTAAAAA AATGCAACAC TATGCGCATG CAGCCATGAA TCTGATCAAC 
ATGGACTCGG AAAACAGGGT GGTGCTCAAT GTGGGTGGCA TTAGGCACGA AACCTACAAG 
GCCACGCTGA AGAAGATTCC GGCTACGCGA TTATCGCGAT TAACAGAGGC GCTGGCCAAC 
TATGATCCGA TACTGAATGA GTACTTCTTT GATCGGCATC CGGGCGTCTT CGCACAAGTG 
CTCAACTATT ACAGAACTGG AAAGCTGCAT TATCCCACGG ATGTGTGCGG TCCGCTGTTT 
GAGGAGGAAT TGGAGTTCTG GGGCCTAGAC TCGAACCAAG TGGAGCCCTG CTGTTGGATG 
ACCTACACAC AGCATCGCGA CACCCAGGAA ACCCTAGCCG TACTCGATCG TCTCGATCTG 
GATACGGAAA AACCGTCCGA AGAGGAATTG GCACGCAAAT TCGGCTTCGA GGAGGACTAC 
TACAAAGGCA CAATATCCTG GTGGCAGGAA ATGAAGCCGC GCATTTGGTC CTTGTTCGAT 
GAGCCCTACA GTTCCAATGC AGCCAAGACT ATTGGCGTGG TTTCGGTGTT CTTCATCTGC 
ATTTCGATCC TGTCGTTCTG CCTGAAGACC CATCCCGATA TGCGGGTACC CATCGTCCGG 
AACATTACAG TGAAAACTGC GAATGGAAGT AATGGCTGGT TTTTGGACAA AACGCAGACC 
AATGCGCACA TAGCCTTCTT CTATATCGAA TGCGTGTGCA ATGCCTGGTT TACCTTTGAA 
ATATTGGTGC GCTTTATCTC ATCGCCGAAC AAGTGGGAGT TCATCAAGTC ATCTGTTAAC 
ATCATAGACT ACATAGCGAC GCTTAGTTTT TATATCGATC TAGTGCTTCA GCGGTTCGCA 
TCGCACCTGG AGAACGCTGA CATCCTCGAG TTCTTCTCGA TCATCCGCAT CATGCGTCTG 
TTCAAGCTGA CGCGCCACTC GTCCGGACTG AAGATCCTGA TCCAGACGTT CCGGGCCTCG 
GCCAAGGAGC TGACCCTGCT GGTGTTCTTC CTCGTCCTGG GCATCGTGAT CTTCGCCAGC 
CTTGTCTACT ACGCGGAGCG CATCCAGCCC AATCCGCACA ACGACTTCAA CAGCATACCG 
CTGGGCCTGT GGTGGGCCCT GGTCACAATG ACCACCGTCG GCTACGGCGA CATGGCCCCC 
AAAACCTACA TCGGCATGTT CGTGGGCGCC CTCTGCGCCC TGGCCGGCGT ACTAACCATC 
GCACTGCCAG TGCCCGTCAT CGTCAGCAAC TTCGCCATGT ACTACTCGCA CACGCAGGCC 
AGGGCCAAAC TGCCAAAGAA GCGGAGACGA GTGCTTCCCG TCGAGCAGCC GCGCCAGCCC 
AGACTGCCAG GTGCCCCTGG TGGTGTCAGT GGTTGCGGTA CCCCGGGCTC GGGTCCCCAC 
TCCGGTCCCA TGGGATCCGG CGGTACTGGA CCACGTCGCA TGAACAATAA AACAAAGGAC 
CTGGTCAGCC CCAAGTCAGT TGCTCAACTT TTCGCAGGTC CGCTGGGCGC AAGCATTGTG 
GCCATGAGTC CCCGGACAAT GCTGGATCTC AATCCTGCCC TGGCGATGGG CAAGCCTACG 
TTTCAGCCCC GTATACCCAC CCCCCTGGCA GCCACGCCCC CTCCCCCCGT GAGTTCCGCT 
GGGGGGATGA CAGCGTCTGG AATTGGAACT ACTTCAGCCA CTGGAGCCAC TAGTGCACCA 
CAACCAGCAA CACCACTACC CAGCATTGCG GTGTCCACCA CAGCGAGTGT GGGCAAGGAT 
TTGGGCATCT CGACCACCAC CACCACGGCC CAGGAGACCA GCAAGAAGGC CTTCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137782.1
CDS	1..1860
Translation	MTYIPKKMQHYAHAAMNLINMDSENRVVLNVGGIRHETYKATLKKIPATRLSRLTEALANYDPILNEYFFDRHPGVFAQVLNYYRTGKLHYPTDVCGPLFEEELEFWGLDSNQVEPCCWMTYTQHRDTQETLAVLDRLDLDTEKPSEEELARKFGFEEDYYKGTISWWQEMKPRIWSLFDEPYSSNAAKTIGVVSVFFICISILSFCLKTHPDMRVPIVRNITVKTANGSNGWFLDKTQTNAHIAFFYIECVCNAWFTFEILVRFISSPNKWEFIKSSVNIIDYIATLSFYIDLVLQRFASHLENADILEFFSIIRIMRLFKLTRHSSGLKILIQTFRASAKELTLLVFFLVLGIVIFASLVYYAERIQPNPHNDFNSIPLGLWWALVTMTTVGYGDMAPKTYIGMFVGALCALAGVLTIALPVPVIVSNFAMYYSHTQARAKLPKKRRRVLPVEQPRQPRLPGAPGGVSGCGTPGSGPHSGPMGSGGTGPRRMNNKTKDLVSPKSVAQLFAGPLGASIVAMSPRTMLDLNPALAMGKPTFQPRIPTPLAATPPPPVSSAGGMTASGIGTTSATGATSAPQPATPLPSIAVSTTASVGKDLGISTTTTTAQETSKKAFL

Target ORF information:

RefSeq Version	NM_001144310.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaker cognate w, transcript variant C (Shaw), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144310.1

ORF Insert Sequence:

ATGACTTATA TACCTAAAAA AATGCAACAC TATGCGCATG CAGCCATGAA TCTGATCAAC 
ATGGACTCGG AAAACAGGGT GGTGCTCAAT GTGGGTGGCA TTAGGCACGA AACCTACAAG 
GCCACGCTGA AGAAGATTCC GGCTACGCGA TTATCGCGAT TAACAGAGGC GCTGGCCAAC 
TATGATCCGA TACTGAATGA GTACTTCTTT GATCGGCATC CGGGCGTCTT CGCACAAGTG 
CTCAACTATT ACAGAACTGG AAAGCTGCAT TATCCCACGG ATGTGTGCGG TCCGCTGTTT 
GAGGAGGAAT TGGAGTTCTG GGGCCTAGAC TCGAACCAAG TGGAGCCCTG CTGTTGGATG 
ACCTACACAC AGCATCGCGA CACCCAGGAA ACCCTAGCCG TACTCGATCG TCTCGATCTG 
GATACGGAAA AACCGTCCGA AGAGGAATTG GCACGCAAAT TCGGCTTCGA GGAGGACTAC 
TACAAAGGCA CAATATCCTG GTGGCAGGAA ATGAAGCCGC GCATTTGGTC CTTGTTCGAT 
GAGCCCTACA GTTCCAATGC AGCCAAGACT ATTGGCGTGG TTTCGGTGTT CTTCATCTGC 
ATTTCGATCC TGTCGTTCTG CCTGAAGACC CATCCCGATA TGCGGGTACC CATCGTCCGG 
AACATTACAG TGAAAACTGC GAATGGAAGT AATGGCTGGT TTTTGGACAA AACGCAGACC 
AATGCGCACA TAGCCTTCTT CTATATCGAA TGCGTGTGCA ATGCCTGGTT TACCTTTGAA 
ATATTGGTGC GCTTTATCTC ATCGCCGAAC AAGTGGGAGT TCATCAAGTC ATCTGTTAAC 
ATCATAGACT ACATAGCGAC GCTTAGTTTT TATATCGATC TAGTGCTTCA GCGGTTCGCA 
TCGCACCTGG AGAACGCTGA CATCCTCGAG TTCTTCTCGA TCATCCGCAT CATGCGTCTG 
TTCAAGCTGA CGCGCCACTC GTCCGGACTG AAGATCCTGA TCCAGACGTT CCGGGCCTCG 
GCCAAGGAGC TGACCCTGCT GGTGTTCTTC CTCGTCCTGG GCATCGTGAT CTTCGCCAGC 
CTTGTCTACT ACGCGGAGCG CATCCAGCCC AATCCGCACA ACGACTTCAA CAGCATACCG 
CTGGGCCTGT GGTGGGCCCT GGTCACAATG ACCACCGTCG GCTACGGCGA CATGGCCCCC 
AAAACCTACA TCGGCATGTT CGTGGGCGCC CTCTGCGCCC TGGCCGGCGT ACTAACCATC 
GCACTGCCAG TGCCCGTCAT CGTCAGCAAC TTCGCCATGT ACTACTCGCA CACGCAGGCC 
AGGGCCAAAC TGCCAAAGAA GCGGAGACGA GTGCTTCCCG TCGAGCAGCC GCGCCAGCCC 
AGACTGCCAG GTGCCCCTGG TGGTGTCAGT GGTTGCGGTA CCCCGGGCTC GGGTCCCCAC 
TCCGGTCCCA TGGGATCCGG CGGTACTGGA CCACGTCGCA TGAACAATAA AACAAAGGAC 
CTGGTCAGCC CCAAGTCAGT TGCTCAACTT TTCGCAGGTC CGCTGGGCGC AAGCATTGTG 
GCCATGAGTC CCCGGACAAT GCTGGATCTC AATCCTGCCC TGGCGATGGG CAAGCCTACG 
TTTCAGCCCC GTATACCCAC CCCCCTGGCA GCCACGCCCC CTCCCCCCGT GAGTTCCGCT 
GGGGGGATGA CAGCGTCTGG AATTGGAACT ACTTCAGCCA CTGGAGCCAC TAGTGCACCA 
CAACCAGCAA CACCACTACC CAGCATTGCG GTGTCCACCA CAGCGAGTGT GGGCAAGGAT 
TTGGGCATCT CGACCACCAC CACCACGGCC CAGGAGACCA GCAAGAAGGC CTTCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shaw ( NM_001273072.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260001.1 Drosophila melanogaster shaker cognate w, transcript variant E (Shaw), mRNA.

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CloneID	OFa16163
Clone ID Related Accession (Same CDS sequence)	NM_001273072.1
Accession Version	NM_001273072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaker cognate w, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTGA TCAACATGGA CTCGGAAAAC AGGGTGGTGC TCAATGTGGG TGGCATTAGG 
CACGAAACCT ACAAGGCCAC GCTGAAGAAG ATTCCGGCTA CGCGATTATC GCGATTAACA 
GAGGCGCTGG CCAACTATGA TCCGATACTG AATGAGTACT TCTTTGATCG GCATCCGGGC 
GTCTTCGCAC AAGTGCTCAA CTATTACAGA ACTGGAAAGC TGCATTATCC CACGGATGTG 
TGCGGTCCGC TGTTTGAGGA GGAATTGGAG TTCTGGGGCC TAGACTCGAA CCAAGTGGAG 
CCCTGCTGTT GGATGACCTA CACACAGCAT CGCGACACCC AGGAAACCCT AGCCGTACTC 
GATCGTCTCG ATCTGGATAC GGAAAAACCG TCCGAAGAGG AATTGGCACG CAAATTCGGC 
TTCGAGGAGG ACTACTACAA AGGCACAATA TCCTGGTGGC AGGAAATGAA GCCGCGCATT 
TGGTCCTTGT TCGATGAGCC CTACAGTTCC AATGCAGCCA AGACTATTGG CGTGGTTTCG 
GTGTTCTTCA TCTGCATTTC GATCCTGTCG TTCTGCCTGA AGACCCATCC CGATATGCGG 
GTACCCATCG TCCGGAACAT TACAGTGAAA ACTGCGAATG GAAGTAATGG CTGGTTTTTG 
GACAAAACGC AGACCAATGC GCACATAGCC TTCTTCTATA TCGAATGCGT GTGCAATGCC 
TGGTTTACCT TTGAAATATT GGTGCGCTTT ATCTCATCGC CGAACAAGTG GGAGTTCATC 
AAGTCATCTG TTAACATCAT AGACTACATA GCGACGCTTA GTTTTTATAT CGATCTAGTG 
CTTCAGCGGT TCGCATCGCA CCTGGAGAAC GCTGACATCC TCGAGTTCTT CTCGATCATC 
CGCATCATGC GTCTGTTCAA GCTGACGCGC CACTCGTCCG GACTGAAGAT CCTGATCCAG 
ACGTTCCGGG CCTCGGCCAA GGAGCTGACC CTGCTGGTGT TCTTCCTCGT CCTGGGCATC 
GTGATCTTCG CCAGCCTTGT CTACTACGCG GAGCGCATCC AGCCCAATCC GCACAACGAC 
TTCAACAGCA TACCGCTGGG CCTGTGGTGG GCCCTGGTCA CAATGACCAC CGTCGGCTAC 
GGCGACATGG CCCCCAAAAC CTACATCGGC ATGTTCGTGG GCGCCCTCTG CGCCCTGGCC 
GGCGTACTAA CCATCGCACT GCCAGTGCCC GTCATCGTCA GCAACTTCGC CATGTACTAC 
TCGCACACGC AGGCCAGGGC CAAACTGCCA AAGAAGCGGA GACGAGTGCT TCCCGTCGAG 
CAGCCGCGCC AGCCCAGACT GCCAGGTGCC CCTGGTGGTG TCAGTGGTTG CGGTACCCCG 
GGCTCGGGTC CCCACTCCGG TCCCATGGGA TCCGGCGGTA CTGGACCACG TCGCATGAAC 
AATAAAACAA AGGACCTGGT CAGCCCCAAG TCAGGTCCGC TGGGCGCAAG CATTGTGGCC 
ATGAGTCCCC GGACAATGCT GGATCTCAAT CCTGCCCTGG CGATGGGCAA GCCTACGTTT 
CAGCCCCGTA TACCCACCCC CCTGGCAGCC ACGCCCCCTC CCCCCGTGAG TTCCGCTGGG 
GGGATGACAG CGTCTGGAAT TGGAACTACT TCAGCCACTG GAGCCACTAG TGCACCACAA 
CCAGCAACAC CACTACCCAG CATTGCGGTG TCCACCACAG CGAGTGTGGG CAAGGATTTG 
GGCATCTCGA CCACCACCAC CACGGCCCAG GAGACCAGCA AGAAGGCCTT CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260001.1
CDS	458..2254
Translation	MNLINMDSENRVVLNVGGIRHETYKATLKKIPATRLSRLTEALANYDPILNEYFFDRHPGVFAQVLNYYRTGKLHYPTDVCGPLFEEELEFWGLDSNQVEPCCWMTYTQHRDTQETLAVLDRLDLDTEKPSEEELARKFGFEEDYYKGTISWWQEMKPRIWSLFDEPYSSNAAKTIGVVSVFFICISILSFCLKTHPDMRVPIVRNITVKTANGSNGWFLDKTQTNAHIAFFYIECVCNAWFTFEILVRFISSPNKWEFIKSSVNIIDYIATLSFYIDLVLQRFASHLENADILEFFSIIRIMRLFKLTRHSSGLKILIQTFRASAKELTLLVFFLVLGIVIFASLVYYAERIQPNPHNDFNSIPLGLWWALVTMTTVGYGDMAPKTYIGMFVGALCALAGVLTIALPVPVIVSNFAMYYSHTQARAKLPKKRRRVLPVEQPRQPRLPGAPGGVSGCGTPGSGPHSGPMGSGGTGPRRMNNKTKDLVSPKSGPLGASIVAMSPRTMLDLNPALAMGKPTFQPRIPTPLAATPPPPVSSAGGMTASGIGTTSATGATSAPQPATPLPSIAVSTTASVGKDLGISTTTTTAQETSKKAFL

Target ORF information:

RefSeq Version	NM_001273072.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaker cognate w, transcript variant E (Shaw), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273072.1

ORF Insert Sequence:

ATGAATCTGA TCAACATGGA CTCGGAAAAC AGGGTGGTGC TCAATGTGGG TGGCATTAGG 
CACGAAACCT ACAAGGCCAC GCTGAAGAAG ATTCCGGCTA CGCGATTATC GCGATTAACA 
GAGGCGCTGG CCAACTATGA TCCGATACTG AATGAGTACT TCTTTGATCG GCATCCGGGC 
GTCTTCGCAC AAGTGCTCAA CTATTACAGA ACTGGAAAGC TGCATTATCC CACGGATGTG 
TGCGGTCCGC TGTTTGAGGA GGAATTGGAG TTCTGGGGCC TAGACTCGAA CCAAGTGGAG 
CCCTGCTGTT GGATGACCTA CACACAGCAT CGCGACACCC AGGAAACCCT AGCCGTACTC 
GATCGTCTCG ATCTGGATAC GGAAAAACCG TCCGAAGAGG AATTGGCACG CAAATTCGGC 
TTCGAGGAGG ACTACTACAA AGGCACAATA TCCTGGTGGC AGGAAATGAA GCCGCGCATT 
TGGTCCTTGT TCGATGAGCC CTACAGTTCC AATGCAGCCA AGACTATTGG CGTGGTTTCG 
GTGTTCTTCA TCTGCATTTC GATCCTGTCG TTCTGCCTGA AGACCCATCC CGATATGCGG 
GTACCCATCG TCCGGAACAT TACAGTGAAA ACTGCGAATG GAAGTAATGG CTGGTTTTTG 
GACAAAACGC AGACCAATGC GCACATAGCC TTCTTCTATA TCGAATGCGT GTGCAATGCC 
TGGTTTACCT TTGAAATATT GGTGCGCTTT ATCTCATCGC CGAACAAGTG GGAGTTCATC 
AAGTCATCTG TTAACATCAT AGACTACATA GCGACGCTTA GTTTTTATAT CGATCTAGTG 
CTTCAGCGGT TCGCATCGCA CCTGGAGAAC GCTGACATCC TCGAGTTCTT CTCGATCATC 
CGCATCATGC GTCTGTTCAA GCTGACGCGC CACTCGTCCG GACTGAAGAT CCTGATCCAG 
ACGTTCCGGG CCTCGGCCAA GGAGCTGACC CTGCTGGTGT TCTTCCTCGT CCTGGGCATC 
GTGATCTTCG CCAGCCTTGT CTACTACGCG GAGCGCATCC AGCCCAATCC GCACAACGAC 
TTCAACAGCA TACCGCTGGG CCTGTGGTGG GCCCTGGTCA CAATGACCAC CGTCGGCTAC 
GGCGACATGG CCCCCAAAAC CTACATCGGC ATGTTCGTGG GCGCCCTCTG CGCCCTGGCC 
GGCGTACTAA CCATCGCACT GCCAGTGCCC GTCATCGTCA GCAACTTCGC CATGTACTAC 
TCGCACACGC AGGCCAGGGC CAAACTGCCA AAGAAGCGGA GACGAGTGCT TCCCGTCGAG 
CAGCCGCGCC AGCCCAGACT GCCAGGTGCC CCTGGTGGTG TCAGTGGTTG CGGTACCCCG 
GGCTCGGGTC CCCACTCCGG TCCCATGGGA TCCGGCGGTA CTGGACCACG TCGCATGAAC 
AATAAAACAA AGGACCTGGT CAGCCCCAAG TCAGGTCCGC TGGGCGCAAG CATTGTGGCC 
ATGAGTCCCC GGACAATGCT GGATCTCAAT CCTGCCCTGG CGATGGGCAA GCCTACGTTT 
CAGCCCCGTA TACCCACCCC CCTGGCAGCC ACGCCCCCTC CCCCCGTGAG TTCCGCTGGG 
GGGATGACAG CGTCTGGAAT TGGAACTACT TCAGCCACTG GAGCCACTAG TGCACCACAA 
CCAGCAACAC CACTACCCAG CATTGCGGTG TCCACCACAG CGAGTGTGGG CAAGGATTTG 
GGCATCTCGA CCACCACCAC CACGGCCCAG GAGACCAGCA AGAAGGCCTT CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shaw ( NM_001273071.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260000.1 Drosophila melanogaster shaker cognate w, transcript variant D (Shaw), mRNA.

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CloneID	OFa16164
Clone ID Related Accession (Same CDS sequence)	NM_001273071.1
Accession Version	NM_001273071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaker cognate w, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTGA TCAACATGGA CTCGGAAAAC AGGGTGGTGC TCAATGTGGG TGGCATTAGG 
CACGAAACCT ACAAGGCCAC GCTGAAGAAG ATTCCGGCTA CGCGATTATC GCGATTAACA 
GAGGCGCTGG CCAACTATGA TCCGATACTG AATGAGTACT TCTTTGATCG GCATCCGGGC 
GTCTTCGCAC AAGTGCTCAA CTATTACAGA ACTGGAAAGC TGCATTATCC CACGGATGTG 
TGCGGTCCGC TGTTTGAGGA GGAATTGGAG TTCTGGGGCC TAGACTCGAA CCAAGTGGAG 
CCCTGCTGTT GGATGACCTA CACACAGCAT CGCGACACCC AGGAAACCCT AGCCGTACTC 
GATCGTCTCG ATCTGGATAC GGAAAAACCG TCCGAAGAGG AATTGGCACG CAAATTCGGC 
TTCGAGGAGG ACTACTACAA AGGCACAATA TCCTGGTGGC AGGAAATGAA GCCGCGCATT 
TGGTCCTTGT TCGATGAGCC CTACAGTTCC AATGCAGCCA AGACTATTGG CGTGGTTTCG 
GTGTTCTTCA TCTGCATTTC GATCCTGTCG TTCTGCCTGA AGACCCATCC CGATATGCGG 
GTACCCATCG TCCGGAACAT TACAGTGAAA ACTGCGAATG GAAGTAATGG CTGGTTTTTG 
GACAAAACGC AGACCAATGC GCACATAGCC TTCTTCTATA TCGAATGCGT GTGCAATGCC 
TGGTTTACCT TTGAAATATT GGTGCGCTTT ATCTCATCGC CGAACAAGTG GGAGTTCATC 
AAGTCATCTG TTAACATCAT AGACTACATA GCGACGCTTA GTTTTTATAT CGATCTAGTG 
CTTCAGCGGT TCGCATCGCA CCTGGAGAAC GCTGACATCC TCGAGTTCTT CTCGATCATC 
CGCATCATGC GTCTGTTCAA GCTGACGCGC CACTCGTCCG GACTGAAGAT CCTGATCCAG 
ACGTTCCGGG CCTCGGCCAA GGAGCTGACC CTGCTGGTGT TCTTCCTCGT CCTGGGCATC 
GTGATCTTCG CCAGCCTTGT CTACTACGCG GAGCGCATCC AGCCCAATCC GCACAACGAC 
TTCAACAGCA TACCGCTGGG CCTGTGGTGG GCCCTGGTCA CAATGACCAC CGTCGGCTAC 
GGCGACATGG CCCCCAAAAC CTACATCGGC ATGTTCGTGG GCGCCCTCTG CGCCCTGGCC 
GGCGTACTAA CCATCGCACT GCCAGTGCCC GTCATCGTCA GCAACTTCGC CATGTACTAC 
TCGCACACGC AGGCCAGGGC CAAACTGCCA AAGAAGCGGA GACGAGTGCT TCCCGTCGAG 
CAGCCGCGCC AGCCCAGACT GCCAGGTGCC CCTGGTGGTG TCAGTGGTTG CGGTACCCCG 
GGCTCGGGTC CCCACTCCGG TCCCATGGGA TCCGGCGGTA CTGGACCACG TCGCATGAAC 
AATAAAACAA AGGACCTGGT CAGCCCCAAG TCAGATGAAT ATACAACAAT GATTGGCAAA 
CCGCGCGATG GCAAAAAGAG CAGGCTGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260000.1
CDS	304..1836
Translation	MNLINMDSENRVVLNVGGIRHETYKATLKKIPATRLSRLTEALANYDPILNEYFFDRHPGVFAQVLNYYRTGKLHYPTDVCGPLFEEELEFWGLDSNQVEPCCWMTYTQHRDTQETLAVLDRLDLDTEKPSEEELARKFGFEEDYYKGTISWWQEMKPRIWSLFDEPYSSNAAKTIGVVSVFFICISILSFCLKTHPDMRVPIVRNITVKTANGSNGWFLDKTQTNAHIAFFYIECVCNAWFTFEILVRFISSPNKWEFIKSSVNIIDYIATLSFYIDLVLQRFASHLENADILEFFSIIRIMRLFKLTRHSSGLKILIQTFRASAKELTLLVFFLVLGIVIFASLVYYAERIQPNPHNDFNSIPLGLWWALVTMTTVGYGDMAPKTYIGMFVGALCALAGVLTIALPVPVIVSNFAMYYSHTQARAKLPKKRRRVLPVEQPRQPRLPGAPGGVSGCGTPGSGPHSGPMGSGGTGPRRMNNKTKDLVSPKSDEYTTMIGKPRDGKKSRLL

Target ORF information:

RefSeq Version	NM_001273071.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaker cognate w, transcript variant D (Shaw), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273071.1

ORF Insert Sequence:

ATGAATCTGA TCAACATGGA CTCGGAAAAC AGGGTGGTGC TCAATGTGGG TGGCATTAGG 
CACGAAACCT ACAAGGCCAC GCTGAAGAAG ATTCCGGCTA CGCGATTATC GCGATTAACA 
GAGGCGCTGG CCAACTATGA TCCGATACTG AATGAGTACT TCTTTGATCG GCATCCGGGC 
GTCTTCGCAC AAGTGCTCAA CTATTACAGA ACTGGAAAGC TGCATTATCC CACGGATGTG 
TGCGGTCCGC TGTTTGAGGA GGAATTGGAG TTCTGGGGCC TAGACTCGAA CCAAGTGGAG 
CCCTGCTGTT GGATGACCTA CACACAGCAT CGCGACACCC AGGAAACCCT AGCCGTACTC 
GATCGTCTCG ATCTGGATAC GGAAAAACCG TCCGAAGAGG AATTGGCACG CAAATTCGGC 
TTCGAGGAGG ACTACTACAA AGGCACAATA TCCTGGTGGC AGGAAATGAA GCCGCGCATT 
TGGTCCTTGT TCGATGAGCC CTACAGTTCC AATGCAGCCA AGACTATTGG CGTGGTTTCG 
GTGTTCTTCA TCTGCATTTC GATCCTGTCG TTCTGCCTGA AGACCCATCC CGATATGCGG 
GTACCCATCG TCCGGAACAT TACAGTGAAA ACTGCGAATG GAAGTAATGG CTGGTTTTTG 
GACAAAACGC AGACCAATGC GCACATAGCC TTCTTCTATA TCGAATGCGT GTGCAATGCC 
TGGTTTACCT TTGAAATATT GGTGCGCTTT ATCTCATCGC CGAACAAGTG GGAGTTCATC 
AAGTCATCTG TTAACATCAT AGACTACATA GCGACGCTTA GTTTTTATAT CGATCTAGTG 
CTTCAGCGGT TCGCATCGCA CCTGGAGAAC GCTGACATCC TCGAGTTCTT CTCGATCATC 
CGCATCATGC GTCTGTTCAA GCTGACGCGC CACTCGTCCG GACTGAAGAT CCTGATCCAG 
ACGTTCCGGG CCTCGGCCAA GGAGCTGACC CTGCTGGTGT TCTTCCTCGT CCTGGGCATC 
GTGATCTTCG CCAGCCTTGT CTACTACGCG GAGCGCATCC AGCCCAATCC GCACAACGAC 
TTCAACAGCA TACCGCTGGG CCTGTGGTGG GCCCTGGTCA CAATGACCAC CGTCGGCTAC 
GGCGACATGG CCCCCAAAAC CTACATCGGC ATGTTCGTGG GCGCCCTCTG CGCCCTGGCC 
GGCGTACTAA CCATCGCACT GCCAGTGCCC GTCATCGTCA GCAACTTCGC CATGTACTAC 
TCGCACACGC AGGCCAGGGC CAAACTGCCA AAGAAGCGGA GACGAGTGCT TCCCGTCGAG 
CAGCCGCGCC AGCCCAGACT GCCAGGTGCC CCTGGTGGTG TCAGTGGTTG CGGTACCCCG 
GGCTCGGGTC CCCACTCCGG TCCCATGGGA TCCGGCGGTA CTGGACCACG TCGCATGAAC 
AATAAAACAA AGGACCTGGT CAGCCCCAAG TCAGATGAAT ATACAACAAT GATTGGCAAA 
CCGCGCGATG GCAAAAAGAG CAGGCTGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Shaw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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