Pif1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pif1
Entrez Gene ID	33567
Full Name	CG3238 gene product from transcript CG3238-RB
Synonyms	Dmel\CG3238
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23F3-23F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_134938.3	NP_608782.1	Pif1 ortholog, isoform A
NM_001298658.1	NP_001285587.1	Pif1 ortholog, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pif1	NP_766041.1
Caenorhabditis elegans (roundworm)	pif-1	NP_490774.2
Xenopus tropicalis (tropical clawed frog)	pif1	NP_001016603.1
Homo sapiens (human)	PIF1	NP_001273426.1
	PIF1	XP_001108233.1
Gallus gallus (chicken)	PIF1	XP_426648.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007775	XP_317740.4
Xenopus tropicalis (tropical clawed frog)	LOC101730335	XP_004914011.1
Bos taurus (cattle)	PIF1	NP_001192981.1
Rattus norvegicus (Norway rat)	Pif1	NP_001037718.1
Danio rerio (zebrafish)	pif1	NP_942102.1
Pan troglodytes (chimpanzee)	PIF1	XP_510475.2
Canis lupus familiaris (dog)	PIF1	XP_003640106.1
Drosophila melanogaster (fruit fly)	CG3238	NP_608782.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pif1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pif1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13646	NM_134938.3 Latest version!	Drosophila melanogaster Pif1 ortholog (Pif1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13646	NM_001298658.1 Latest version!	Drosophila melanogaster Pif1 ortholog (Pif1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pif1 ( NM_134938.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608782.1 Drosophila melanogaster Pif1 ortholog (Pif1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13646
Clone ID Related Accession (Same CDS sequence)	NM_134938.3 , NM_001298658.1
Accession Version	NM_134938.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pif1 ortholog, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_134938.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCTCA ATGACGCAGT GCTCACCTGT GCGGTGAACA TGGAGTGGAC CAATGCGGTG 
GGCATCACGG GTCGCAAACT GGCCTACAAG ACTGCCACAT TGCGCCTGGT GCGGAACGAT 
TTGCGTGAAT TGTTTGTTGA GGTGACGCCG GAGAAACTAA AGCCCCTGAA GTTCAAGCTG 
AAGGATCTGA TGGTGCACAA GAAGTTCATG TCCGAGGGCA AGGCCACCTT CAACTTCAAG 
GCGGAGAAGT GCACCCTCTA TCTATCGAAT GCACCGCCGG GGACTTTGAT GTTCTTCCTG 
CGGACGATCT TCGTTAAAAT GAACGGAAAC GAGGGAGGAT CCCAGCCGGA CGATGCGACC 
TTGCAGAAGA AACTCCGCGA GCACCTGATG TCAGGCAAGC CAAGCAGCTT TAACGACGTT 
TCTCCAGTCA CCACGGCCGA GATGATTTTG GCGCGCAAAA AGGCGGGTTT GTTGTCCAAG 
GGGTCAGTGA CCACGCCGTC GCCCCAGGGC GCCAAAAAGC GTCGCTTCGA GGAGCTCAAG 
GAGGAGAAGG AACGGGGCAC CATGCCCGCG GCCAAGAAAC TATATGCGTC GACTACCGAC 
GAGTCGCTGA GACTCAGTGA GGAGCAAATG GAAGTCCTGC GGGCGTGCAC CTCGGGCAAG 
AGCGTATTCT TCACCGGTTC AGCAGGCACT GGCAAGAGTT TCTTGCTCCG CAGGATCATC 
TCAGCCCTGC CGCCCGATGG AACGGTGGCC ACGGCCTCCA CGGGAGTGGC AGCCTGCCTG 
ATCGGTGGGA CCACCTTGCA CGCCTTCGCT GGCATAGGAG GAGGAGACGC CACCATGCAG 
CGGTGTCTGG AGCTGGCTTC CCGACCCGCA AATGCCCAGA CGTGGAGGAA GTGCAAGCGG 
TTGATTATTG ACGAGATCTC CATGGTGGAC GGACAGTTCT TTGAGAAAAT TGAAGCCGTC 
GCACGTCACA TTCGGCGCAA TGATCGCCCC TTTGGCGGCA TACAGCTCAT CCTGTGTGGT 
GACTTCTTGC AACTGCCTCC GGTCATAAAG GGAGATTTTG GTGCAGCCCC CACTGCCACG 
CCCCAGCAAC GATTCTGCTT TCAGTCCTCC GCATGGGAGA CTTGCATCCA GTGTGTCTAC 
GAACTGAAGC AAGTGCATCG GCAATCGGAT CCGGAGTTTG TAAAGATTCT GAACCACCTC 
CGAATTGGCC ATGTGAACGA TTCCATAACC AGTCGACTTG CAGCTACATC AAAGCAGAAA 
ATTGAGGGAA ACGGCATTCT GGCCACTCAA CTCTGCTCCC ACACAAACGA TGCGAACTCC 
ATTAACGAGT CCAAGCTGGA AAACCTCGAT GGCGACAAGA TTTTATTCAA GGCTGACGAC 
TCCGATGCCA GCATGACCAG GACACTGGAT CAACAGATCC AGGCTCCATC TCAGTTGTAC 
CTAAAAGTTA ACGCCCAAGT AATGCTACTC AAGAATATAA ACATATCCAA TGGACTGGTG 
AACGGAGCTC GAGGCGTCGT TGTAAGAATG GAGAAGGATC TGCCAGTGGT AAGGTTCAAA 
AACAACCAGG AATACGTTTG CAAGCACGAA AGGTGGATAA TAAAGACCGC CTCAGGAAAC 
CATATAACAC GACGCCAAGT GCCACTGAAG TTAGCCTGGG CATTTTCCAT ACACAAGAGT 
CAGGGACTAA CCCTCGACTG CGTGGAGATG TCCCTTTCCA AAGTATTTGA GGCTGGACAG 
GCCTATGTGG CCTTATCCCG TGCGAAATCC CTGCAGTCCA TTCGCATCCT GGACTTTGAC 
GCCAAGCAAG TGTGGGCCAA TCCACACGTG CTGCAGTTCT ACAAGGGATT CAGACGCAAG 
CTAATGGACA CAACCATGAT ACCATTGGGT CCCAAAAACA AGGACAAGAA GCCAGGAGAC 
AAGGCCGCAA ATAGCGCTCT GGCTAAGCTC AAAAAGAGTC TCATCAACAA GCCGCTGGTC 
TCGATTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608782.1
CDS	111..2102
Translation	MDLNDAVLTCAVNMEWTNAVGITGRKLAYKTATLRLVRNDLRELFVEVTPEKLKPLKFKLKDLMVHKKFMSEGKATFNFKAEKCTLYLSNAPPGTLMFFLRTIFVKMNGNEGGSQPDDATLQKKLREHLMSGKPSSFNDVSPVTTAEMILARKKAGLLSKGSVTTPSPQGAKKRRFEELKEEKERGTMPAAKKLYASTTDESLRLSEEQMEVLRACTSGKSVFFTGSAGTGKSFLLRRIISALPPDGTVATASTGVAACLIGGTTLHAFAGIGGGDATMQRCLELASRPANAQTWRKCKRLIIDEISMVDGQFFEKIEAVARHIRRNDRPFGGIQLILCGDFLQLPPVIKGDFGAAPTATPQQRFCFQSSAWETCIQCVYELKQVHRQSDPEFVKILNHLRIGHVNDSITSRLAATSKQKIEGNGILATQLCSHTNDANSINESKLENLDGDKILFKADDSDASMTRTLDQQIQAPSQLYLKVNAQVMLLKNINISNGLVNGARGVVVRMEKDLPVVRFKNNQEYVCKHERWIIKTASGNHITRRQVPLKLAWAFSIHKSQGLTLDCVEMSLSKVFEAGQAYVALSRAKSLQSIRILDFDAKQVWANPHVLQFYKGFRRKLMDTTMIPLGPKNKDKKPGDKAANSALAKLKKSLINKPLVSIS

Target ORF information:

RefSeq Version	NM_134938.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pif1 ortholog (Pif1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134938.3

ORF Insert Sequence:

ATGGATCTCA ATGACGCAGT GCTCACCTGT GCGGTGAACA TGGAGTGGAC CAATGCGGTG 
GGCATCACGG GTCGCAAACT GGCCTACAAG ACTGCCACAT TGCGCCTGGT GCGGAACGAT 
TTGCGTGAAT TGTTTGTTGA GGTGACGCCG GAGAAACTAA AGCCCCTGAA GTTCAAGCTG 
AAGGATCTGA TGGTGCACAA GAAGTTCATG TCCGAGGGCA AGGCCACCTT CAACTTCAAG 
GCGGAGAAGT GCACCCTCTA TCTATCGAAT GCACCGCCGG GGACTTTGAT GTTCTTCCTG 
CGGACGATCT TCGTTAAAAT GAACGGAAAC GAGGGAGGAT CCCAGCCGGA CGATGCGACC 
TTGCAGAAGA AACTCCGCGA GCACCTGATG TCAGGCAAGC CAAGCAGCTT TAACGACGTT 
TCTCCAGTCA CCACGGCCGA GATGATTTTG GCGCGCAAAA AGGCGGGTTT GTTGTCCAAG 
GGGTCAGTGA CCACGCCGTC GCCCCAGGGC GCCAAAAAGC GTCGCTTCGA GGAGCTCAAG 
GAGGAGAAGG AACGGGGCAC CATGCCCGCG GCCAAGAAAC TATATGCGTC GACTACCGAC 
GAGTCGCTGA GACTCAGTGA GGAGCAAATG GAAGTCCTGC GGGCGTGCAC CTCGGGCAAG 
AGCGTATTCT TCACCGGTTC AGCAGGCACT GGCAAGAGTT TCTTGCTCCG CAGGATCATC 
TCAGCCCTGC CGCCCGATGG AACGGTGGCC ACGGCCTCCA CGGGAGTGGC AGCCTGCCTG 
ATCGGTGGGA CCACCTTGCA CGCCTTCGCT GGCATAGGAG GAGGAGACGC CACCATGCAG 
CGGTGTCTGG AGCTGGCTTC CCGACCCGCA AATGCCCAGA CGTGGAGGAA GTGCAAGCGG 
TTGATTATTG ACGAGATCTC CATGGTGGAC GGACAGTTCT TTGAGAAAAT TGAAGCCGTC 
GCACGTCACA TTCGGCGCAA TGATCGCCCC TTTGGCGGCA TACAGCTCAT CCTGTGTGGT 
GACTTCTTGC AACTGCCTCC GGTCATAAAG GGAGATTTTG GTGCAGCCCC CACTGCCACG 
CCCCAGCAAC GATTCTGCTT TCAGTCCTCC GCATGGGAGA CTTGCATCCA GTGTGTCTAC 
GAACTGAAGC AAGTGCATCG GCAATCGGAT CCGGAGTTTG TAAAGATTCT GAACCACCTC 
CGAATTGGCC ATGTGAACGA TTCCATAACC AGTCGACTTG CAGCTACATC AAAGCAGAAA 
ATTGAGGGAA ACGGCATTCT GGCCACTCAA CTCTGCTCCC ACACAAACGA TGCGAACTCC 
ATTAACGAGT CCAAGCTGGA AAACCTCGAT GGCGACAAGA TTTTATTCAA GGCTGACGAC 
TCCGATGCCA GCATGACCAG GACACTGGAT CAACAGATCC AGGCTCCATC TCAGTTGTAC 
CTAAAAGTTA ACGCCCAAGT AATGCTACTC AAGAATATAA ACATATCCAA TGGACTGGTG 
AACGGAGCTC GAGGCGTCGT TGTAAGAATG GAGAAGGATC TGCCAGTGGT AAGGTTCAAA 
AACAACCAGG AATACGTTTG CAAGCACGAA AGGTGGATAA TAAAGACCGC CTCAGGAAAC 
CATATAACAC GACGCCAAGT GCCACTGAAG TTAGCCTGGG CATTTTCCAT ACACAAGAGT 
CAGGGACTAA CCCTCGACTG CGTGGAGATG TCCCTTTCCA AAGTATTTGA GGCTGGACAG 
GCCTATGTGG CCTTATCCCG TGCGAAATCC CTGCAGTCCA TTCGCATCCT GGACTTTGAC 
GCCAAGCAAG TGTGGGCCAA TCCACACGTG CTGCAGTTCT ACAAGGGATT CAGACGCAAG 
CTAATGGACA CAACCATGAT ACCATTGGGT CCCAAAAACA AGGACAAGAA GCCAGGAGAC 
AAGGCCGCAA ATAGCGCTCT GGCTAAGCTC AAAAAGAGTC TCATCAACAA GCCGCTGGTC 
TCGATTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1 ( NM_001298658.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285587.1 Drosophila melanogaster Pif1 ortholog (Pif1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13646
Clone ID Related Accession (Same CDS sequence)	NM_134938.3 , NM_001298658.1
Accession Version	NM_001298658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pif1 ortholog, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCTCA ATGACGCAGT GCTCACCTGT GCGGTGAACA TGGAGTGGAC CAATGCGGTG 
GGCATCACGG GTCGCAAACT GGCCTACAAG ACTGCCACAT TGCGCCTGGT GCGGAACGAT 
TTGCGTGAAT TGTTTGTTGA GGTGACGCCG GAGAAACTAA AGCCCCTGAA GTTCAAGCTG 
AAGGATCTGA TGGTGCACAA GAAGTTCATG TCCGAGGGCA AGGCCACCTT CAACTTCAAG 
GCGGAGAAGT GCACCCTCTA TCTATCGAAT GCACCGCCGG GGACTTTGAT GTTCTTCCTG 
CGGACGATCT TCGTTAAAAT GAACGGAAAC GAGGGAGGAT CCCAGCCGGA CGATGCGACC 
TTGCAGAAGA AACTCCGCGA GCACCTGATG TCAGGCAAGC CAAGCAGCTT TAACGACGTT 
TCTCCAGTCA CCACGGCCGA GATGATTTTG GCGCGCAAAA AGGCGGGTTT GTTGTCCAAG 
GGGTCAGTGA CCACGCCGTC GCCCCAGGGC GCCAAAAAGC GTCGCTTCGA GGAGCTCAAG 
GAGGAGAAGG AACGGGGCAC CATGCCCGCG GCCAAGAAAC TATATGCGTC GACTACCGAC 
GAGTCGCTGA GACTCAGTGA GGAGCAAATG GAAGTCCTGC GGGCGTGCAC CTCGGGCAAG 
AGCGTATTCT TCACCGGTTC AGCAGGCACT GGCAAGAGTT TCTTGCTCCG CAGGATCATC 
TCAGCCCTGC CGCCCGATGG AACGGTGGCC ACGGCCTCCA CGGGAGTGGC AGCCTGCCTG 
ATCGGTGGGA CCACCTTGCA CGCCTTCGCT GGCATAGGAG GAGGAGACGC CACCATGCAG 
CGGTGTCTGG AGCTGGCTTC CCGACCCGCA AATGCCCAGA CGTGGAGGAA GTGCAAGCGG 
TTGATTATTG ACGAGATCTC CATGGTGGAC GGACAGTTCT TTGAGAAAAT TGAAGCCGTC 
GCACGTCACA TTCGGCGCAA TGATCGCCCC TTTGGCGGCA TACAGCTCAT CCTGTGTGGT 
GACTTCTTGC AACTGCCTCC GGTCATAAAG GGAGATTTTG GTGCAGCCCC CACTGCCACG 
CCCCAGCAAC GATTCTGCTT TCAGTCCTCC GCATGGGAGA CTTGCATCCA GTGTGTCTAC 
GAACTGAAGC AAGTGCATCG GCAATCGGAT CCGGAGTTTG TAAAGATTCT GAACCACCTC 
CGAATTGGCC ATGTGAACGA TTCCATAACC AGTCGACTTG CAGCTACATC AAAGCAGAAA 
ATTGAGGGAA ACGGCATTCT GGCCACTCAA CTCTGCTCCC ACACAAACGA TGCGAACTCC 
ATTAACGAGT CCAAGCTGGA AAACCTCGAT GGCGACAAGA TTTTATTCAA GGCTGACGAC 
TCCGATGCCA GCATGACCAG GACACTGGAT CAACAGATCC AGGCTCCATC TCAGTTGTAC 
CTAAAAGTTA ACGCCCAAGT AATGCTACTC AAGAATATAA ACATATCCAA TGGACTGGTG 
AACGGAGCTC GAGGCGTCGT TGTAAGAATG GAGAAGGATC TGCCAGTGGT AAGGTTCAAA 
AACAACCAGG AATACGTTTG CAAGCACGAA AGGTGGATAA TAAAGACCGC CTCAGGAAAC 
CATATAACAC GACGCCAAGT GCCACTGAAG TTAGCCTGGG CATTTTCCAT ACACAAGAGT 
CAGGGACTAA CCCTCGACTG CGTGGAGATG TCCCTTTCCA AAGTATTTGA GGCTGGACAG 
GCCTATGTGG CCTTATCCCG TGCGAAATCC CTGCAGTCCA TTCGCATCCT GGACTTTGAC 
GCCAAGCAAG TGTGGGCCAA TCCACACGTG CTGCAGTTCT ACAAGGGATT CAGACGCAAG 
CTAATGGACA CAACCATGAT ACCATTGGGT CCCAAAAACA AGGACAAGAA GCCAGGAGAC 
AAGGCCGCAA ATAGCGCTCT GGCTAAGCTC AAAAAGAGTC TCATCAACAA GCCGCTGGTC 
TCGATTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285587.1
CDS	111..2102
Translation	MDLNDAVLTCAVNMEWTNAVGITGRKLAYKTATLRLVRNDLRELFVEVTPEKLKPLKFKLKDLMVHKKFMSEGKATFNFKAEKCTLYLSNAPPGTLMFFLRTIFVKMNGNEGGSQPDDATLQKKLREHLMSGKPSSFNDVSPVTTAEMILARKKAGLLSKGSVTTPSPQGAKKRRFEELKEEKERGTMPAAKKLYASTTDESLRLSEEQMEVLRACTSGKSVFFTGSAGTGKSFLLRRIISALPPDGTVATASTGVAACLIGGTTLHAFAGIGGGDATMQRCLELASRPANAQTWRKCKRLIIDEISMVDGQFFEKIEAVARHIRRNDRPFGGIQLILCGDFLQLPPVIKGDFGAAPTATPQQRFCFQSSAWETCIQCVYELKQVHRQSDPEFVKILNHLRIGHVNDSITSRLAATSKQKIEGNGILATQLCSHTNDANSINESKLENLDGDKILFKADDSDASMTRTLDQQIQAPSQLYLKVNAQVMLLKNINISNGLVNGARGVVVRMEKDLPVVRFKNNQEYVCKHERWIIKTASGNHITRRQVPLKLAWAFSIHKSQGLTLDCVEMSLSKVFEAGQAYVALSRAKSLQSIRILDFDAKQVWANPHVLQFYKGFRRKLMDTTMIPLGPKNKDKKPGDKAANSALAKLKKSLINKPLVSIS

Target ORF information:

RefSeq Version	NM_001298658.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pif1 ortholog (Pif1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298658.1

ORF Insert Sequence:

ATGGATCTCA ATGACGCAGT GCTCACCTGT GCGGTGAACA TGGAGTGGAC CAATGCGGTG 
GGCATCACGG GTCGCAAACT GGCCTACAAG ACTGCCACAT TGCGCCTGGT GCGGAACGAT 
TTGCGTGAAT TGTTTGTTGA GGTGACGCCG GAGAAACTAA AGCCCCTGAA GTTCAAGCTG 
AAGGATCTGA TGGTGCACAA GAAGTTCATG TCCGAGGGCA AGGCCACCTT CAACTTCAAG 
GCGGAGAAGT GCACCCTCTA TCTATCGAAT GCACCGCCGG GGACTTTGAT GTTCTTCCTG 
CGGACGATCT TCGTTAAAAT GAACGGAAAC GAGGGAGGAT CCCAGCCGGA CGATGCGACC 
TTGCAGAAGA AACTCCGCGA GCACCTGATG TCAGGCAAGC CAAGCAGCTT TAACGACGTT 
TCTCCAGTCA CCACGGCCGA GATGATTTTG GCGCGCAAAA AGGCGGGTTT GTTGTCCAAG 
GGGTCAGTGA CCACGCCGTC GCCCCAGGGC GCCAAAAAGC GTCGCTTCGA GGAGCTCAAG 
GAGGAGAAGG AACGGGGCAC CATGCCCGCG GCCAAGAAAC TATATGCGTC GACTACCGAC 
GAGTCGCTGA GACTCAGTGA GGAGCAAATG GAAGTCCTGC GGGCGTGCAC CTCGGGCAAG 
AGCGTATTCT TCACCGGTTC AGCAGGCACT GGCAAGAGTT TCTTGCTCCG CAGGATCATC 
TCAGCCCTGC CGCCCGATGG AACGGTGGCC ACGGCCTCCA CGGGAGTGGC AGCCTGCCTG 
ATCGGTGGGA CCACCTTGCA CGCCTTCGCT GGCATAGGAG GAGGAGACGC CACCATGCAG 
CGGTGTCTGG AGCTGGCTTC CCGACCCGCA AATGCCCAGA CGTGGAGGAA GTGCAAGCGG 
TTGATTATTG ACGAGATCTC CATGGTGGAC GGACAGTTCT TTGAGAAAAT TGAAGCCGTC 
GCACGTCACA TTCGGCGCAA TGATCGCCCC TTTGGCGGCA TACAGCTCAT CCTGTGTGGT 
GACTTCTTGC AACTGCCTCC GGTCATAAAG GGAGATTTTG GTGCAGCCCC CACTGCCACG 
CCCCAGCAAC GATTCTGCTT TCAGTCCTCC GCATGGGAGA CTTGCATCCA GTGTGTCTAC 
GAACTGAAGC AAGTGCATCG GCAATCGGAT CCGGAGTTTG TAAAGATTCT GAACCACCTC 
CGAATTGGCC ATGTGAACGA TTCCATAACC AGTCGACTTG CAGCTACATC AAAGCAGAAA 
ATTGAGGGAA ACGGCATTCT GGCCACTCAA CTCTGCTCCC ACACAAACGA TGCGAACTCC 
ATTAACGAGT CCAAGCTGGA AAACCTCGAT GGCGACAAGA TTTTATTCAA GGCTGACGAC 
TCCGATGCCA GCATGACCAG GACACTGGAT CAACAGATCC AGGCTCCATC TCAGTTGTAC 
CTAAAAGTTA ACGCCCAAGT AATGCTACTC AAGAATATAA ACATATCCAA TGGACTGGTG 
AACGGAGCTC GAGGCGTCGT TGTAAGAATG GAGAAGGATC TGCCAGTGGT AAGGTTCAAA 
AACAACCAGG AATACGTTTG CAAGCACGAA AGGTGGATAA TAAAGACCGC CTCAGGAAAC 
CATATAACAC GACGCCAAGT GCCACTGAAG TTAGCCTGGG CATTTTCCAT ACACAAGAGT 
CAGGGACTAA CCCTCGACTG CGTGGAGATG TCCCTTTCCA AAGTATTTGA GGCTGGACAG 
GCCTATGTGG CCTTATCCCG TGCGAAATCC CTGCAGTCCA TTCGCATCCT GGACTTTGAC 
GCCAAGCAAG TGTGGGCCAA TCCACACGTG CTGCAGTTCT ACAAGGGATT CAGACGCAAG 
CTAATGGACA CAACCATGAT ACCATTGGGT CCCAAAAACA AGGACAAGAA GCCAGGAGAC 
AAGGCCGCAA ATAGCGCTCT GGCTAAGCTC AAAAAGAGTC TCATCAACAA GCCGCTGGTC 
TCGATTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pif1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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