Nipped-B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nipped-B
Entrez Gene ID	3355136
Full Name	CG17704 gene product from transcript CG17704-RG
Synonyms	CG17704,CG40281,Dmel\CG17704,NIPPED-B,NipB,l(2)02047,nipped-B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 41B3-41C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042986.3	NP_001036451.1	Nipped-B, isoform F
NM_001273774.1	NP_001260703.1	Nipped-B, isoform J
NM_001169583.2	NP_001163054.1	Nipped-B, isoform I
NM_001169582.2	NP_001163053.1	Nipped-B, isoform H
NM_001042987.3	NP_001036452.1	Nipped-B, isoform E
NM_001169581.2	NP_001163052.1	Nipped-B, isoform G

Pathways from BioSystems

Homologies

	NIPBL	XP_001096575.1
Rattus norvegicus (Norway rat)	Nipbl	XP_003749266.1
Drosophila melanogaster (fruit fly)	Nipped-B	NP_001163052.1
Homo sapiens (human)	NIPBL	NP_597677.2
Canis lupus familiaris (dog)	NIPBL	XP_005619439.1
Bos taurus (cattle)	NIPBL	NP_001193513.1
Mus musculus (house mouse)	Nipbl	NP_081983.2
Gallus gallus (chicken)	LOC427025	NP_001244277.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009290	XP_320088.4
Pan troglodytes (chimpanzee)	NIPBL	XP_001146090.1
Gallus gallus (chicken)	LOC427439	XP_425012.3
Danio rerio (zebrafish)	nipblb	NP_001154919.2
Xenopus tropicalis (tropical clawed frog)	nipbl	XP_002938229.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

studies confirm that Drosophila Nipped-B mutants provide a useful model for understanding CdLS, and provide new insights into the origins of birth defects
Title: Drosophila Nipped-B Mutants Model Cornelia de Lange Syndrome in Growth and Behavior.

Nipped-B and Pds5 regulate transcription by controlling how much cohesin binds DNA in the stable mode, and not binding affinity.
Title: Dosage-sensitive regulation of cohesin chromosome binding and dynamics by Nipped-B, Pds5, and Wapl.

Nipped-B & cohesin co-localize on chromosomes, & bind actively transcribed genes & control regulatory sequences, like transcriptional enhancers & insulators. They may define active chromatin domains & influence transcriptional elongation. Review.
Title: Cohesin, gene expression and development: lessons from Drosophila.

Data show that Nipped-B is required for sister chromatid cohesion but that, opposite to the effect of Nipped-B, the stromalin/Scc3 component of Cohesin inhibits long-range activation of cut.
Title: Drosophila nipped-B protein supports sister chromatid cohesion and opposes the stromalin/Scc3 cohesion factor to facilitate long-range activation of the cut gene.

The following Nipped-B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nipped-B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13074	NM_001273774.1 Latest version!	Drosophila melanogaster Nipped-B, transcript variant J (Nipped-B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa11829	NM_001042986.3 Latest version!	Drosophila melanogaster Nipped-B, transcript variant F (Nipped-B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa11829	NM_001042987.3 Latest version!	Drosophila melanogaster Nipped-B, transcript variant E (Nipped-B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa13073	NM_001169581.2 Latest version!	Drosophila melanogaster Nipped-B, transcript variant G (Nipped-B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa13073	NM_001169583.2 Latest version!	Drosophila melanogaster Nipped-B, transcript variant I (Nipped-B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa13073	NM_001169582.2 Latest version!	Drosophila melanogaster Nipped-B, transcript variant H (Nipped-B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nipped-B ( NM_001273774.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260703.1 Drosophila melanogaster Nipped-B, transcript variant J (Nipped-B), mRNA.

Price & Availability↑

CloneID	OFa13074
Clone ID Related Accession (Same CDS sequence)	NM_001273774.1
Accession Version	NM_001273774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nipped-B, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTACT CTCCGGACCA ACATACTGCT AATGCTCGAT TAGAGGCAAA GAAAATGCAA 
ATTGATTCCT ATTCAATACC GGAATGTTAT GCATCACCAA CAAATTTGTC GATTTCAAAT 
GAACATCAAT TGCAAGACGC TCAAGCCTGC TTCAATCAGC TTACTAGTAT GACCTCAAAA 
GAAATCTTAG AAGAATTTTC ACAAATAAAT TTTAAGGAAA ATGCAACAGA AAAAGATAAA 
ATTGACGTTA TTGAAAATTC AGATTTAAAT GTGACTAAGA TACTTTCACA AAATACTCTA 
AAAAAAAAGT CTAACACACC AGAAAGAAGT ACGGTGCAAT CAATACAAGA ACAGTTTTTT 
ATTCAGCAAC AAGAAGCCAA TTATAATTTA AAACATGATT TAAACCAAGT ATCAACAAAT 
GTTGGTCAAA TTCAAAATAT TTCTGATACT TTTCCCCAAG AGCCGTCAAA TTTTAATTTA 
AATTCTGTAA ATATCCCAAA CATGTTATAT GTACAAAGTC CACCTTTCAC TGAATCAGAA 
CCAACTCAAG CACACCACTC TTCAATTGAA TATTTAAAAA AAAAAAAATC ACAAATTCTT 
GACGTCAGCA TTTTAAATCG ACAACAGGTG TTAGAAAATA CCAGCTTACA TATTATTAAT 
AAAAGTTCAA CGTACCAAAC GAACCAAAAT GTATCAACAA CTACATCGAC ATCTACTTCC 
TCAAGTGGCA AGTCCCAGGT CCGGTCACCA GAATTGGCTA GAAGCATGGG GTTACTACAA 
GAAACGTGCC AGCAGCAACA TGAAAACTTG AATATTATAC CCACGAGTGC GGAAGAATGT 
GTATTAGAGA GTCGTGCTTC CAGTACAAAT AATACAGTCA AAATCCCTAT TGATACATTT 
TCTACTATAA AAGCCGATGA AAAACGATCC GCGAAAAGAA AACTTGCCAT ATCAATTGGT 
GACATCCCAC CGGAACAAAT ATTTTCAAAG CCTAAAATGC GTCGGGTCGA AAGAATTACA 
CCTCTTTCGA ACACAAAGTG TGTGAAGGAG GAAGTCACCC GTTCGCAAAC ATATCAACAA 
TTTATTCGAA ACATGGATCA CATAATTGAA ATATTAGACG ATAGTGAATC TCCTAACTTT 
GACTCAGAGG ATGTAGACGA AACCATTGAA TGCATTTCCT CAAAACTTTT AAATTCAATG 
AGTACTGATG TTGCCAAGTT AAAAGCTAAG CAGGCTTTAG ATTCAATACC AAAAAATAAG 
TTAACCCTAC TTATTAACTA TGCAATGCGA AACGTGTACT TAGCCCGGAA TTATTTTGCT 
GGAACGGAGG ACGAAGATGA ATTTGTAGAT GATGAAGTTA TTGAAAAATT ACTTAACGCA 
ATGGATGCTT GCTTGCTTAT ATGTAACATT TATTCTACAG TAAGCGACCT TCAGTTCTTA 
CAAGAAGATA ACGTATCACA CATTATAAAA TTTACGCAAT TTCAACTGCG TGAAACAATT 
TTTCCTCTTC ATGACCCGGT TTACACGGCA AAAAGTATAA AAAGGACAAC TCATCGTAAA 
AAAATAAAAT CACACCAAGC TCAAAATCGA AGTATGCAGC TATTCTACTT GAAAACTGTA 
GAGCTATTAA AAGTTTTTGT TACACTTTTT GACAAATGTG TATTTGTTGA TACTATAGTT 
TTACCATTGT CGACACTGGC TATTGAACCA TTTTTTGTTG ACAACATAGA GACGTTGCAA 
TTTGTATGTT TGGAACTCGT TACCACAATT TTTCGCAAGG AAAGATATGA TAAAATAAGA 
AACAGTATCT TAGGCGACAT TTTGACATCT ATTGATCGGT TACCTTCATC AAAAAAAAAT 
CTCCGCCCAT ATAAACTCAC AAACAATGGC GGAAATATTC AAATGGTTAC AGCACTTGTA 
TTGCAATTGA TTCAATGCGC CACGATTTTA CCAGATTCCC TTTGTGATAA TGGCAAATTT 
AGTAACAAAC CCCAAGAGGG AAATACTTTT GATGAAGAAG GAAAAAAATT GCTTCAGCCT 
AGTCAGGATC TACTCGTTTT ACAAAAATAT GATGTAGCTG TCAGCATTGG TGGAAATTTT 
TTAACAACTT TCCTTAACAA ATGCAAATCT CGGTCTAATG AAACTGATTT TCGACCTCTG 
TTTGAAAATT TTATTCATGA TTTACTGGCC ACCGTGAATA AGCCTGAATG GCCTGCATCT 
GAATTGTTGC TTTCGCTGTT GGGAACTATG CTGGTTCGCT ACGTTTCTGA TAAGGGTATA 
GAACAAAGCA TTCGCTTGGT ATCATTGGAT TACCTGGGCA TAGTAGCTGC TCGATTGCGA 
AAAGACACAG TGGAATCTCG GTGTAGAGTT AACATTATTG ATTCAATGAT TCAATCCATC 
AAATTAGAGC AAGAAAAGGA GGGAGATGTA ACATCAAACA ATGATCAATT TGATCTAGAA 
CCCGAGGAGC AGCGAACGGA TTTTTTGCAA AAGATTCTTC TCGACTTTCT AGCTGTAAAT 
GCTCAAGAAG AAAACTTAAT CTGGGACTAT GCTAGACATT TTTATTTGGC ACAATGGTAT 
CGGGATGTCA TATATCAAAG ACGTCGGATA AATGATGGCA AAAAAGGTTT GGCTTTCAGG 
AAGTCCAAAA TTCGTAATAA TCGGAGGACT AATGGTGATT ATTTAGATAC GTCGGATTCT 
GGATCGTGCG ATGAAAGTGA TACCGATACA AACAAAAAGA GAATTCACTG TGTGGACAGC 
AATGATTTCG AACTAAATAT TAACATCTAT AAGGCTTTAG AAGCAAGAAA GCAATATTTT 
ATCAACAAAA TTAAGCCATT TTCAGTTTTT GGCGAGCAAA ACCACTCGTC AAATCAACAC 
ATCAAAACAT ACATAGATTA TAATAACGCA CAGTTAATAG CACAATATCT GGCCACCAAA 
AGGCCCTTCA GTCAATCTTT TGATGGATGC TTAAAGAAAA TTATATTAGT TGTTAATGAG 
CCATCTATAG CAGTAAGGAC ACGTGCAATG AAATGTCTTG CCAACATAGT GGAAGTTGAT 
CCATTGGTTC TAAAACGAAA AGATATGCAA ATGGGAGTTA ATCAGAAATT CCTAGACACA 
GCTATTTCGG TACGAGAAGC TGCAGTAGAT TTGGTTGGAA AATTTGTTCT AAGTAATCAA 
GATCTAATCG ATCAGTATTA CGATATGCTG TCTACTCGTA TTTTGGATAC TGGTGTCTCC 
GTACGGAAAA GGGTCATTAA AATTTTGAGA GATATTTGTT TAGAATATCC AGACTTTTCA 
AAAATTCCCG AAATTTGCGT TAAAATGATT CGACGTGTTC ATGACGAAGA AGGCATACAA 
AAACTTGTCA CTGAAGTTTT TATGAAGATG TGGTTTACAC CTTGTACTAA AAACGACAAA 
ATTGGAATAC AAAGGAAAAT AAATCATATA ATTGACGTGG TAAATACTGC ACATGATACT 
GGAACTACTT GGTTAGAGGG CCTTCTAATG AGTATATTTA AGCCAAGAGA TAATATGCTA 
AGGTCTGAAG GATGCGTTCA AGAATTTATT AAGAAGAACT CAGAGCCACC AATGGATATT 
GTGATAGCTT GTCAACAATT AGCCGACGGA CTAGTTGACA GACTAATTGA ATTGGAAGAT 
ACAGATAACT CGCGAATGCT GGGTTGTATT ACCACACTTC ATTTACTGGC TAAAGTGCGG 
CCCCAACTGC TTGTTAAGCA TGCTATAACA ATTGAACCAT ATCTCAACAT AAAATGTCAT 
TCTGCCACAG CCGCCAAGTT CATATGTGCA GTTGCAGATA TTCTCGAAAA AGTAGTTCCT 
CTGGTTAATA ATGCCAGTGA ATCCTTTTTA GCATCGTTGG AAGAGCATTT AATGTTATTG 
GTTGTTTCAC GAAATCAAGC CGAAGTTACT AGCTGTGTTT CTTGCTTGGG AGCACTTGTG 
AATAAGATTA CACATAACTT TAAATTGATC CGCGACTGTT TTCAAAAATT TTATCGGGTT 
CTTGATGTGT CTCGAAGTCA AGTAATACAA GGAAATAACA GCGTAGATAA TATATACACT 
CCCAGCTTTC GGCGAAGTTT GTTTACGATC GGAATACTAA TGCGATATTT CGACTTTAAG 
TCTCCTATTG CGTTAGGTGA AACAAATGAT GGACTTCCTG TTTCTATATG TGAGGATGTA 
TTCCATTGCT TGATGTTCTT CTGCCGTTGT ACTAATCAAG AAATTCGAAA GCAAGCACTT 
ATTTCGCTTG GATCATTTTG TGTACTAAAC GATGGCTACC TTACAAGATC CGAACTTAAA 
AATTTGTATT GCGAAATTTT AAGTTCAATA GCCAACGACG CCGGCTTTAA AATAATCTGC 
ATGCGAAATA TTTGGATTTA TCTAACGGAA TCTGAAATGT TTATGCACAA TAAGGAAAAA 
GAATGGGAAA AACAATCAAA ACACGAAGAT CTTAAGGAAA TGAACGACGT TTCGTCTGGC 
ATGGCAAGTC GAATAATTCA ACTATATTTA GAAGAAATTC TTGAATGCTT TCTTAATCGG 
GATGACACAG TTCGGTTATG GGCTGTCAAA GTAATACAGA TTGTCCTCCG TCAAGGTTTG 
GTTCACCCTG TTAGAATGGT TCCGTATTTA ATTTGCCTGA GTACTGATCA TAGGATAGAG 
TCTGCTCATC GAGCTGATGC CCTGTTGAAA GATATTGATA AAACATACTC TGGATTTGTT 
AATATGAAAG TGCAATTCGG GCTACAGCTT TGCTTTAAGC TACAAAAAAT TTTACAAATA 
AACAACAGAG GGAAACTGGA AATAATTAGA GGTTATGCGA GTCGTGGACC AGATAATACA 
ACTACCGCTT TAAACGATTT TCTGTATACA CTTCTTCGCA CTACTAAACC GCAAAGGCGT 
GCTTTGGTGC AAACAGTTAC AAAACAGTTT GACGATCAGA AAACATCATT ACAACAAATG 
TTATATATTG CAGATAACCT GGCTTACTTT CCATATGTAG TTCAAGATGA ACCTTTATAT 
CTAATCCATC AAATAGATCT TCTAATTTCT ATGGCTGGAA CACATTTACT TGCTACATTC 
AAGGAACATA TAAAACCAAG CGACAAGGAA GGAGATGTTT TAGAAGATGA TGATGACGTA 
GAAGATCCAG AGGTGTTGTT TAATAGATTG CCCGAAGATT TGACGGAAAT TATTAAATGT 
ATAACATCTG CTCAAGCATG TATGCTTCTT TTAATACTGA AAGATCATCT GAAGGAAATG 
TATGCGATTA CTGACAGTAA AATTTCAAGA TATTCACCTT CCGAACAAAA ATTATATGAA 
AAAGCCGTAA CACGTAAAAG TGTTAATGAT TTTAACCCAA AAACGACTAT TGATGTTATA 
AAGAAACAAA TGTCGCAAGA GAAATTGAGT ACGGACATTA ATTTTACACT GACTAAAGAG 
GAGAAATTGG ACCTTGTTGT TAAATACCTT GATTTTAAGC AACTGATGCT TAAATTGGAT 
CCGGATGATG GAGACTCCGA TGCTGATGAA TCCCGCGACA AAACAATGTT AAATATCTCT 
GCTTCTTCGG ATGGAGTGGC ATTCTCTAGT TCAGCAAAAA ATTCTCACTC CGCTTGTGAT 
GGATACTCAC TAACAGTCAC GGATGTGGTT GATGTCCCAA TGTCTCATAT TGCGAAGGCA 
TCTATGTTGA CTTCAAAACC TAGTGGTCGC AAAACAAATC CTGTGCGAAC TAAAAAAAAA 
AGAAGAAAAA TTGATTCTAC GGATGATGAA ACCAGTGATG CGGAATATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260703.1
CDS	292..6165
Translation	MGERDNPTVPVTTLAGLTSTSDYSPDQHTANARLEAKKMQIDSYSIPECYASPTNLSISNEHQLQDAQACFNQLTSMTSKEILEEFSQINFKENATEKDKIDVIENSDLNVTKILSQNTLKKKSNTPERSTVQSIQEQFFIQQQEANYNLKHDLNQVSTNVGQIQNISDTFPQEPSNFNLNSVNIPNMLYVQSPPFTESEPTQAHHSSIEYLKKKKSQILDVSILNRQQVLENTSLHIINKSSTYQTNQNVSTTTSTSTSSSGKSQVRSPELARSMGLLQETCQQQHENLNIIPTSAEECVLESRASSTNNTVKIPIDTFSTIKADEKRSAKRKLAISIGDIPPEQIFSKPKMRRVERITPLSNTKCVKEEVTRSQTYQQFIRNMDHIIEILDDSESPNFDSEDVDETIECISSKLLNSMSTDVAKLKAKQALDSIPKNKLTLLINYAMRNVYLARNYFAGTEDEDEFVDDEVIEKLLNAMDACLLICNIYSTVSDLQFLQEDNVSHIIKFTQFQLRETIFPLHDPVYTAKSIKRTTHRKKIKSHQAQNRSMQLFYLKTVELLKVFVTLFDKCVFVDTIVLPLSTLAIEPFFVDNIETLQFVCLELVTTIFRKERYDKIRNSILGDILTSIDRLPSSKKNLRPYKLTNNGGNIQMVTALVLQLIQCATILPDSLCDNGKFSNKPQEGNTFDEEGKKLLQPSQDLLVLQKYDVAVSIGGNFLTTFLNKCKSRSNETDFRPLFENFIHDLLATVNKPEWPASELLLSLLGTMLVRYVSDKGIEQSIRLVSLDYLGIVAARLRKDTVESRCRVNIIDSMIQSIKLEQEKEGDVTSNNDQFDLEPEEQRTDFLQKILLDFLAVNAQEENLIWDYARHFYLAQWYRDVIYQRRRINDGKKGLAFRKSKIRNNRRTNGDYLDTSDSGSCDESDTDTNKKRIHCVDSNDFELNINIYKALEARKQYFINKIKPFSVFGEQNHSSNQHIKTYIDYNNAQLIAQYLATKRPFSQSFDGCLKKIILVVNEPSIAVRTRAMKCLANIVEVDPLVLKRKDMQMGVNQKFLDTAISVREAAVDLVGKFVLSNQDLIDQYYDMLSTRILDTGVSVRKRVIKILRDICLEYPDFSKIPEICVKMIRRVHDEEGIQKLVTEVFMKMWFTPCTKNDKIGIQRKINHIIDVVNTAHDTGTTWLEGLLMSIFKPRDNMLRSEGCVQEFIKKNSEPPMDIVIACQQLADGLVDRLIELEDTDNSRMLGCITTLHLLAKVRPQLLVKHAITIEPYLNIKCHSATAAKFICAVADILEKVVPLVNNASESFLASLEEHLMLLVVSRNQAEVTSCVSCLGALVNKITHNFKLIRDCFQKFYRVLDVSRSQVIQGNNSVDNIYTPSFRRSLFTIGILMRYFDFKSPIALGETNDGLPVSICEDVFHCLMFFCRCTNQEIRKQALISLGSFCVLNDGYLTRSELKNLYCEILSSIANDAGFKIICMRNIWIYLTESEMFMHNKEKEWEKQSKHEDLKEMNDVSSGMASRIIQLYLEEILECFLNRDDTVRLWAVKVIQIVLRQGLVHPVRMVPYLICLSTDHRIESAHRADALLKDIDKTYSGFVNMKVQFGLQLCFKLQKILQINNRGKLEIIRGYASRGPDNTTTALNDFLYTLLRTTKPQRRALVQTVTKQFDDQKTSLQQMLYIADNLAYFPYVVQDEPLYLIHQIDLLISMAGTHLLATFKEHIKPSDKEGDVLEDDDDVEDPEVLFNRLPEDLTEIIKCITSAQACMLLLILKDHLKEMYAITDSKISRYSPSEQKLYEKAVTRKSVNDFNPKTTIDVIKKQMSQEKLSTDINFTLTKEEKLDLVVKYLDFKQLMLKLDPDDGDSDADESRDKTMLNISASSDGVAFSSSAKNSHSACDGYSLTVTDVVDVPMSHIAKASMLTSKPSGRKTNPVRTKKKRRKIDSTDDETSDAEYA

Target ORF information:

RefSeq Version	NM_001273774.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nipped-B, transcript variant J (Nipped-B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273774.1

ORF Insert Sequence:

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTACT CTCCGGACCA ACATACTGCT AATGCTCGAT TAGAGGCAAA GAAAATGCAA 
ATTGATTCCT ATTCAATACC GGAATGTTAT GCATCACCAA CAAATTTGTC GATTTCAAAT 
GAACATCAAT TGCAAGACGC TCAAGCCTGC TTCAATCAGC TTACTAGTAT GACCTCAAAA 
GAAATCTTAG AAGAATTTTC ACAAATAAAT TTTAAGGAAA ATGCAACAGA AAAAGATAAA 
ATTGACGTTA TTGAAAATTC AGATTTAAAT GTGACTAAGA TACTTTCACA AAATACTCTA 
AAAAAAAAGT CTAACACACC AGAAAGAAGT ACGGTGCAAT CAATACAAGA ACAGTTTTTT 
ATTCAGCAAC AAGAAGCCAA TTATAATTTA AAACATGATT TAAACCAAGT ATCAACAAAT 
GTTGGTCAAA TTCAAAATAT TTCTGATACT TTTCCCCAAG AGCCGTCAAA TTTTAATTTA 
AATTCTGTAA ATATCCCAAA CATGTTATAT GTACAAAGTC CACCTTTCAC TGAATCAGAA 
CCAACTCAAG CACACCACTC TTCAATTGAA TATTTAAAAA AAAAAAAATC ACAAATTCTT 
GACGTCAGCA TTTTAAATCG ACAACAGGTG TTAGAAAATA CCAGCTTACA TATTATTAAT 
AAAAGTTCAA CGTACCAAAC GAACCAAAAT GTATCAACAA CTACATCGAC ATCTACTTCC 
TCAAGTGGCA AGTCCCAGGT CCGGTCACCA GAATTGGCTA GAAGCATGGG GTTACTACAA 
GAAACGTGCC AGCAGCAACA TGAAAACTTG AATATTATAC CCACGAGTGC GGAAGAATGT 
GTATTAGAGA GTCGTGCTTC CAGTACAAAT AATACAGTCA AAATCCCTAT TGATACATTT 
TCTACTATAA AAGCCGATGA AAAACGATCC GCGAAAAGAA AACTTGCCAT ATCAATTGGT 
GACATCCCAC CGGAACAAAT ATTTTCAAAG CCTAAAATGC GTCGGGTCGA AAGAATTACA 
CCTCTTTCGA ACACAAAGTG TGTGAAGGAG GAAGTCACCC GTTCGCAAAC ATATCAACAA 
TTTATTCGAA ACATGGATCA CATAATTGAA ATATTAGACG ATAGTGAATC TCCTAACTTT 
GACTCAGAGG ATGTAGACGA AACCATTGAA TGCATTTCCT CAAAACTTTT AAATTCAATG 
AGTACTGATG TTGCCAAGTT AAAAGCTAAG CAGGCTTTAG ATTCAATACC AAAAAATAAG 
TTAACCCTAC TTATTAACTA TGCAATGCGA AACGTGTACT TAGCCCGGAA TTATTTTGCT 
GGAACGGAGG ACGAAGATGA ATTTGTAGAT GATGAAGTTA TTGAAAAATT ACTTAACGCA 
ATGGATGCTT GCTTGCTTAT ATGTAACATT TATTCTACAG TAAGCGACCT TCAGTTCTTA 
CAAGAAGATA ACGTATCACA CATTATAAAA TTTACGCAAT TTCAACTGCG TGAAACAATT 
TTTCCTCTTC ATGACCCGGT TTACACGGCA AAAAGTATAA AAAGGACAAC TCATCGTAAA 
AAAATAAAAT CACACCAAGC TCAAAATCGA AGTATGCAGC TATTCTACTT GAAAACTGTA 
GAGCTATTAA AAGTTTTTGT TACACTTTTT GACAAATGTG TATTTGTTGA TACTATAGTT 
TTACCATTGT CGACACTGGC TATTGAACCA TTTTTTGTTG ACAACATAGA GACGTTGCAA 
TTTGTATGTT TGGAACTCGT TACCACAATT TTTCGCAAGG AAAGATATGA TAAAATAAGA 
AACAGTATCT TAGGCGACAT TTTGACATCT ATTGATCGGT TACCTTCATC AAAAAAAAAT 
CTCCGCCCAT ATAAACTCAC AAACAATGGC GGAAATATTC AAATGGTTAC AGCACTTGTA 
TTGCAATTGA TTCAATGCGC CACGATTTTA CCAGATTCCC TTTGTGATAA TGGCAAATTT 
AGTAACAAAC CCCAAGAGGG AAATACTTTT GATGAAGAAG GAAAAAAATT GCTTCAGCCT 
AGTCAGGATC TACTCGTTTT ACAAAAATAT GATGTAGCTG TCAGCATTGG TGGAAATTTT 
TTAACAACTT TCCTTAACAA ATGCAAATCT CGGTCTAATG AAACTGATTT TCGACCTCTG 
TTTGAAAATT TTATTCATGA TTTACTGGCC ACCGTGAATA AGCCTGAATG GCCTGCATCT 
GAATTGTTGC TTTCGCTGTT GGGAACTATG CTGGTTCGCT ACGTTTCTGA TAAGGGTATA 
GAACAAAGCA TTCGCTTGGT ATCATTGGAT TACCTGGGCA TAGTAGCTGC TCGATTGCGA 
AAAGACACAG TGGAATCTCG GTGTAGAGTT AACATTATTG ATTCAATGAT TCAATCCATC 
AAATTAGAGC AAGAAAAGGA GGGAGATGTA ACATCAAACA ATGATCAATT TGATCTAGAA 
CCCGAGGAGC AGCGAACGGA TTTTTTGCAA AAGATTCTTC TCGACTTTCT AGCTGTAAAT 
GCTCAAGAAG AAAACTTAAT CTGGGACTAT GCTAGACATT TTTATTTGGC ACAATGGTAT 
CGGGATGTCA TATATCAAAG ACGTCGGATA AATGATGGCA AAAAAGGTTT GGCTTTCAGG 
AAGTCCAAAA TTCGTAATAA TCGGAGGACT AATGGTGATT ATTTAGATAC GTCGGATTCT 
GGATCGTGCG ATGAAAGTGA TACCGATACA AACAAAAAGA GAATTCACTG TGTGGACAGC 
AATGATTTCG AACTAAATAT TAACATCTAT AAGGCTTTAG AAGCAAGAAA GCAATATTTT 
ATCAACAAAA TTAAGCCATT TTCAGTTTTT GGCGAGCAAA ACCACTCGTC AAATCAACAC 
ATCAAAACAT ACATAGATTA TAATAACGCA CAGTTAATAG CACAATATCT GGCCACCAAA 
AGGCCCTTCA GTCAATCTTT TGATGGATGC TTAAAGAAAA TTATATTAGT TGTTAATGAG 
CCATCTATAG CAGTAAGGAC ACGTGCAATG AAATGTCTTG CCAACATAGT GGAAGTTGAT 
CCATTGGTTC TAAAACGAAA AGATATGCAA ATGGGAGTTA ATCAGAAATT CCTAGACACA 
GCTATTTCGG TACGAGAAGC TGCAGTAGAT TTGGTTGGAA AATTTGTTCT AAGTAATCAA 
GATCTAATCG ATCAGTATTA CGATATGCTG TCTACTCGTA TTTTGGATAC TGGTGTCTCC 
GTACGGAAAA GGGTCATTAA AATTTTGAGA GATATTTGTT TAGAATATCC AGACTTTTCA 
AAAATTCCCG AAATTTGCGT TAAAATGATT CGACGTGTTC ATGACGAAGA AGGCATACAA 
AAACTTGTCA CTGAAGTTTT TATGAAGATG TGGTTTACAC CTTGTACTAA AAACGACAAA 
ATTGGAATAC AAAGGAAAAT AAATCATATA ATTGACGTGG TAAATACTGC ACATGATACT 
GGAACTACTT GGTTAGAGGG CCTTCTAATG AGTATATTTA AGCCAAGAGA TAATATGCTA 
AGGTCTGAAG GATGCGTTCA AGAATTTATT AAGAAGAACT CAGAGCCACC AATGGATATT 
GTGATAGCTT GTCAACAATT AGCCGACGGA CTAGTTGACA GACTAATTGA ATTGGAAGAT 
ACAGATAACT CGCGAATGCT GGGTTGTATT ACCACACTTC ATTTACTGGC TAAAGTGCGG 
CCCCAACTGC TTGTTAAGCA TGCTATAACA ATTGAACCAT ATCTCAACAT AAAATGTCAT 
TCTGCCACAG CCGCCAAGTT CATATGTGCA GTTGCAGATA TTCTCGAAAA AGTAGTTCCT 
CTGGTTAATA ATGCCAGTGA ATCCTTTTTA GCATCGTTGG AAGAGCATTT AATGTTATTG 
GTTGTTTCAC GAAATCAAGC CGAAGTTACT AGCTGTGTTT CTTGCTTGGG AGCACTTGTG 
AATAAGATTA CACATAACTT TAAATTGATC CGCGACTGTT TTCAAAAATT TTATCGGGTT 
CTTGATGTGT CTCGAAGTCA AGTAATACAA GGAAATAACA GCGTAGATAA TATATACACT 
CCCAGCTTTC GGCGAAGTTT GTTTACGATC GGAATACTAA TGCGATATTT CGACTTTAAG 
TCTCCTATTG CGTTAGGTGA AACAAATGAT GGACTTCCTG TTTCTATATG TGAGGATGTA 
TTCCATTGCT TGATGTTCTT CTGCCGTTGT ACTAATCAAG AAATTCGAAA GCAAGCACTT 
ATTTCGCTTG GATCATTTTG TGTACTAAAC GATGGCTACC TTACAAGATC CGAACTTAAA 
AATTTGTATT GCGAAATTTT AAGTTCAATA GCCAACGACG CCGGCTTTAA AATAATCTGC 
ATGCGAAATA TTTGGATTTA TCTAACGGAA TCTGAAATGT TTATGCACAA TAAGGAAAAA 
GAATGGGAAA AACAATCAAA ACACGAAGAT CTTAAGGAAA TGAACGACGT TTCGTCTGGC 
ATGGCAAGTC GAATAATTCA ACTATATTTA GAAGAAATTC TTGAATGCTT TCTTAATCGG 
GATGACACAG TTCGGTTATG GGCTGTCAAA GTAATACAGA TTGTCCTCCG TCAAGGTTTG 
GTTCACCCTG TTAGAATGGT TCCGTATTTA ATTTGCCTGA GTACTGATCA TAGGATAGAG 
TCTGCTCATC GAGCTGATGC CCTGTTGAAA GATATTGATA AAACATACTC TGGATTTGTT 
AATATGAAAG TGCAATTCGG GCTACAGCTT TGCTTTAAGC TACAAAAAAT TTTACAAATA 
AACAACAGAG GGAAACTGGA AATAATTAGA GGTTATGCGA GTCGTGGACC AGATAATACA 
ACTACCGCTT TAAACGATTT TCTGTATACA CTTCTTCGCA CTACTAAACC GCAAAGGCGT 
GCTTTGGTGC AAACAGTTAC AAAACAGTTT GACGATCAGA AAACATCATT ACAACAAATG 
TTATATATTG CAGATAACCT GGCTTACTTT CCATATGTAG TTCAAGATGA ACCTTTATAT 
CTAATCCATC AAATAGATCT TCTAATTTCT ATGGCTGGAA CACATTTACT TGCTACATTC 
AAGGAACATA TAAAACCAAG CGACAAGGAA GGAGATGTTT TAGAAGATGA TGATGACGTA 
GAAGATCCAG AGGTGTTGTT TAATAGATTG CCCGAAGATT TGACGGAAAT TATTAAATGT 
ATAACATCTG CTCAAGCATG TATGCTTCTT TTAATACTGA AAGATCATCT GAAGGAAATG 
TATGCGATTA CTGACAGTAA AATTTCAAGA TATTCACCTT CCGAACAAAA ATTATATGAA 
AAAGCCGTAA CACGTAAAAG TGTTAATGAT TTTAACCCAA AAACGACTAT TGATGTTATA 
AAGAAACAAA TGTCGCAAGA GAAATTGAGT ACGGACATTA ATTTTACACT GACTAAAGAG 
GAGAAATTGG ACCTTGTTGT TAAATACCTT GATTTTAAGC AACTGATGCT TAAATTGGAT 
CCGGATGATG GAGACTCCGA TGCTGATGAA TCCCGCGACA AAACAATGTT AAATATCTCT 
GCTTCTTCGG ATGGAGTGGC ATTCTCTAGT TCAGCAAAAA ATTCTCACTC CGCTTGTGAT 
GGATACTCAC TAACAGTCAC GGATGTGGTT GATGTCCCAA TGTCTCATAT TGCGAAGGCA 
TCTATGTTGA CTTCAAAACC TAGTGGTCGC AAAACAAATC CTGTGCGAAC TAAAAAAAAA 
AGAAGAAAAA TTGATTCTAC GGATGATGAA ACCAGTGATG CGGAATATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipped-B ( NM_001042986.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036451.1 Drosophila melanogaster Nipped-B, transcript variant F (Nipped-B), mRNA.

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CloneID	OFa11829
Clone ID Related Accession (Same CDS sequence)	NM_001042986.3 , NM_001042987.3
Accession Version	NM_001042986.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nipped-B, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001042986.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGGTATGTAT AAATCGCCTT AGTATTGAGG ATTCTCGCCT TATGCAACAG 
AGTATTAAAA AGTTTGTACA AAAGTCACCA GAATTGGCTA GAAGCATGGG GTTACTACAA 
GAAACGTGCC AGCAGCAACA TGAAAACTTG AATATTATAC CCACGAGTGC GGAAGAATGT 
GTATTAGAGA GTCGTGCTTC CAGTACAAAT AATACAGTCA AAATCCCTAT TGATACATTT 
TCTACTATAA AAGCCGATGA AAAACGATCC GCGAAAAGAA AACTTGCCAT ATCAATTGGT 
GACATCCCAC CGGAACAAAT ATTTTCAAAG CCTAAAATGC GTCGGGTCGA AAGAATTACA 
CCTCTTTCGA ACACAAAGTG TGTGAAGGAG GAAGTCACCC GTTCGCAAAC ATATCAACAA 
TTTATTCGAA ACATGGATCA CATAATTGAA ATATTAGACG ATAGTGAATC TCCTAACTTT 
GACTCAGAGG ATGTAGACGA AACCATTGAA TGCATTTCCT CAAAACTTTT AAATTCAATG 
AGTACTGATG TTGCCAAGTT AAAAGCTAAG CAGGCTTTAG ATTCAATACC AAAAAATAAG 
TTAACCCTAC TTATTAACTA TGCAATGCGA AACGTGTACT TAGCCCGGAA TTATTTTGCT 
GGAACGGAGG ACGAAGATGA ATTTGTAGAT GATGAAGTTA TTGAAAAATT ACTTAACGCA 
ATGGATGCTT GCTTGCTTAT ATGTAACATT TATTCTACAG TAAGCGACCT TCAGTTCTTA 
CAAGAAGATA ACGTATCACA CATTATAAAA TTTACGCAAT TTCAACTGCG TGAAACAATT 
TTTCCTCTTC ATGACCCGGT TTACACGGCA AAAAGTATAA AAAGGACAAC TCATCGTAAA 
AAAATAAAAT CACACCAAGC TCAAAATCGA AGTATGCAGC TATTCTACTT GAAAACTGTA 
GAGCTATTAA AAGTTTTTGT TACACTTTTT GACAAATGTG TATTTGTTGA TACTATAGTT 
TTACCATTGT CGACACTGGC TATTGAACCA TTTTTTGTTG ACAACATAGA GACGTTGCAA 
TTTGTATGTT TGGAACTCGT TACCACAATT TTTCGCAAGG AAAGATATGA TAAAATAAGA 
AACAGTATCT TAGGCGACAT TTTGACATCT ATTGATCGGT TACCTTCATC AAAAAAAAAT 
CTCCGCCCAT ATAAACTCAC AAACAATGGC GGAAATATTC AAATGGTTAC AGCACTTGTA 
TTGCAATTGA TTCAATGCGC CACGATTTTA CCAGATTCCC TTTGTGATAA TGGCAAATTT 
AGTAACAAAC CCCAAGAGGG AAATACTTTT GATGAAGAAG GAAAAAAATT GCTTCAGCCT 
AGTCAGGATC TACTCGTTTT ACAAAAATAT GATGTAGCTG TCAGCATTGG TGGAAATTTT 
TTAACAACTT TCCTTAACAA ATGCAAATCT CGGTCTAATG AAACTGATTT TCGACCTCTG 
TTTGAAAATT TTATTCATGA TTTACTGGCC ACCGTGAATA AGCCTGAATG GCCTGCATCT 
GAATTGTTGC TTTCGCTGTT GGGAACTATG CTGGTTCGCT ACGTTTCTGA TAAGGGTATA 
GAACAAAGCA TTCGCTTGGT ATCATTGGAT TACCTGGGCA TAGTAGCTGC TCGATTGCGA 
AAAGACACAG TGGAATCTCG GTGTAGAGTT AACATTATTG ATTCAATGAT TCAATCCATC 
AAATTAGAGC AAGAAAAGGA GGGAGATGTA ACATCAAACA ATGATCAATT TGATCTAGAA 
CCCGAGGAGC AGCGAACGGA TTTTTTGCAA AAGATTCTTC TCGACTTTCT AGCTGTAAAT 
GCTCAAGAAG AAAACTTAAT CTGGGACTAT GCTAGACATT TTTATTTGGC ACAATGGTAT 
CGGGATGTCA TATATCAAAG ACGTCGGATA AATGATGGCA AAAAAGGTTT GGCTTTCAGG 
AAGTCCAAAA TTCGTAATAA TCGGAGGACT AATGGTGATT ATTTAGATAC GTCGGATTCT 
GGATCGTGCG ATGAAAGTGA TACCGATACA AACAAAAAGA GAATTCACTG TGTGGACAGC 
AATGATTTCG AACTAAATAT TAACATCTAT AAGGCTTTAG AAGCAAGAAA GCAATATTTT 
ATCAACAAAA TTAAGCCATT TTCAGTTTTT GGCGAGCAAA ACCACTCGTC AAATCAACAC 
ATCAAAACAT ACATAGATTA TAATAACGCA CAGTTAATAG CACAATATCT GGCCACCAAA 
AGGCCCTTCA GTCAATCTTT TGATGGATGC TTAAAGAAAA TTATATTAGT TGTTAATGAG 
CCATCTATAG CAGTAAGGAC ACGTGCAATG AAATGTCTTG CCAACATAGT GGAAGTTGAT 
CCATTGGTTC TAAAACGAAA AGATATGCAA ATGGGAGTTA ATCAGAAATT CCTAGACACA 
GCTATTTCGG TACGAGAAGC TGCAGTAGAT TTGGTTGGAA AATTTGTTCT AAGTAATCAA 
GATCTAATCG ATCAGTATTA CGATATGCTG TCTACTCGTA TTTTGGATAC TGGTGTCTCC 
GTACGGAAAA GGGTCATTAA AATTTTGAGA GATATTTGTT TAGAATATCC AGACTTTTCA 
AAAATTCCCG AAATTTGCGT TAAAATGATT CGACGTGTTC ATGACGAAGA AGGCATACAA 
AAACTTGTCA CTGAAGTTTT TATGAAGATG TGGTTTACAC CTTGTACTAA AAACGACAAA 
ATTGGAATAC AAAGGAAAAT AAATCATATA ATTGACGTGG TAAATACTGC ACATGATACT 
GGAACTACTT GGTTAGAGGG CCTTCTAATG AGTATATTTA AGCCAAGAGA TAATATGCTA 
AGGTCTGAAG GATGCGTTCA AGAATTTATT AAGAAGAACT CAGAGCCACC AATGGATATT 
GTGATAGCTT GTCAACAATT AGCCGACGGA CTAGTTGACA GACTAATTGA ATTGGAAGAT 
ACAGATAACT CGCGAATGCT GGGTTGTATT ACCACACTTC ATTTACTGGC TAAAGTGCGG 
CCCCAACTGC TTGTTAAGCA TGCTATAACA ATTGAACCAT ATCTCAACAT AAAATGTCAT 
TCTGCCACAG CCGCCAAGTT CATATGTGCA GTTGCAGATA TTCTCGAAAA AGTAGTTCCT 
CTGGTTAATA ATGCCAGTGA ATCCTTTTTA GCATCGTTGG AAGAGCATTT AATGTTATTG 
GTTGTTTCAC GAAATCAAGC CGAAGTTACT AGCTGTGTTT CTTGCTTGGG AGCACTTGTG 
AATAAGATTA CACATAACTT TAAATTGATC CGCGACTGTT TTCAAAAATT TTATCGGGTT 
CTTGATGTGT CTCGAAGTCA AGTAATACAA GGAAATAACA GCGTAGATAA TATATACACT 
CCCAGCTTTC GGCGAAGTTT GTTTACGATC GGAATACTAA TGCGATATTT CGACTTTAAG 
TCTCCTATTG CGTTAGGTGA AACAAATGAT GGACTTCCTG TTTCTATATG TGAGGATGTA 
TTCCATTGCT TGATGTTCTT CTGCCGTTGT ACTAATCAAG AAATTCGAAA GCAAGCACTT 
ATTTCGCTTG GATCATTTTG TGTACTAAAC GATGGCTACC TTACAAGATC CGAACTTAAA 
AATTTGTATT GCGAAATTTT AAGTTCAATA GCCAACGACG CCGGCTTTAA AATAATCTGC 
ATGCGAAATA TTTGGATTTA TCTAACGGAA TCTGAAATGT TTATGCACAA TAAGGAAAAA 
GAATGGGAAA AACAATCAAA ACACGAAGAT CTTAAGGAAA TGAACGACGT TTCGTCTGGC 
ATGGCAAGTC GAATAATTCA ACTATATTTA GAAGAAATTC TTGAATGCTT TCTTAATCGG 
GATGACACAG TTCGGTTATG GGCTGTCAAA GTAATACAGA TTGTCCTCCG TCAAGGTTTG 
GTTCACCCTG TTAGAATGGT TCCGTATTTA ATTTGCCTGA GTACTGATCA TAGGATAGAG 
TCTGCTCATC GAGCTGATGC CCTGTTGAAA GATATTGATA AAACATACTC TGGATTTGTT 
AATATGAAAG TGCAATTCGG GCTACAGCTT TGCTTTAAGC TACAAAAAAT TTTACAAATA 
AACAACAGAG GGAAACTGGA AATAATTAGA GGTTATGCGA GTCGTGGACC AGATAATACA 
ACTACCGCTT TAAACGATTT TCTGTATACA CTTCTTCGCA CTACTAAACC GCAAAGGCGT 
GCTTTGGTGC AAACAGTTAC AAAACAGTTT GACGATCAGA AAACATCATT ACAACAAATG 
TTATATATTG CAGATAACCT GGCTTACTTT CCATATGTAG TTCAAGATGA ACCTTTATAT 
CTAATCCATC AAATAGATCT TCTAATTTCT ATGGCTGGAA CACATTTACT TGCTACATTC 
AAGGAACATA TAAAACCAAG CGACAAGGAA GGAGATGTTT TAGAAGATGA TGATGACGTA 
GAAGATCCAG AGGTGTTGTT TAATAGATTG CCCGAAGATT TGACGGAAAT TATTAAATGT 
ATAACATCTG CTCAAGCATG TATGCTTCTT TTAATACTGA AAGATCATCT GAAGGAAATG 
TATGCGATTA CTGACAGTAA AATTTCAAGA TATTCACCTT CCGAACAAAA ATTATATGAA 
AAAGCCGTAA CACGTAAAAG TGTTAATGAT TTTAACCCAA AAACGACTAT TGATGTTATA 
AAGAAACAAA TGTCGCAAGA GAAATTGAGT ACGGACATTA ATTTTACACT GACTAAAGAG 
GAGAAATTGG ACCTTGTTGT TAAATACCTT GATTTTAAGC AACTGATGCT TAAATTGGAT 
CCGGATGATG GAGACTCCGA TGCTGATGAA TCCCGCGACA AAACAATGTT AAATATCTCT 
GCTTCTTCGG ATGGAGTGGC ATTCTCTAGT TCAGCAAAAA ATTCTCACTC CGCTTGTGAT 
GGATACTCAC TAACAGTCAC GGATGTGGTT GATGTCCCAA TGTCTCATAT TGCGAAGGCA 
TCTATGTTGA CTTCAAAACC TAGTGGTCGC AAAACAAATC CTGTGCGAAC TAAAAAAAAA 
AGAAGAAAAA TTGATTCTAC GGATGATGAA ACCAGTGATG CGGAATATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036451.1
CDS	264..6497
Translation	MGERDNPTVPVTTLAGLTSTSDLLSELPVADSLQSAASLNKSLLFHALVANESSNLLSMRNENLVRQLVTAIERTNSDNIELIHCPVQDTATNCSTYPELLQGIYHFRPAVFNTSIKIHSPDQHTANARLEAKKMQIDSYSIPECYASPTNLSISNEHQLQDAQACFNQLTSMTSKEILEEFSQINFKENATEKDKIDVIENSDLNVTKILSQNTLKKKSNTPERSTVQSIQEQFFIQQQEANYNLKHDLNQVSTNVGQIQNISDTFPQEPSNFNLNSVNIPNMLYVQSPPFTESEPTQAHHSSIEYLKKKKSQILDVSILNRQQVLENTSLHIINKSSTYQTNQNVSTTTSTSTSSSGKSQVRVCINRLSIEDSRLMQQSIKKFVQKSPELARSMGLLQETCQQQHENLNIIPTSAEECVLESRASSTNNTVKIPIDTFSTIKADEKRSAKRKLAISIGDIPPEQIFSKPKMRRVERITPLSNTKCVKEEVTRSQTYQQFIRNMDHIIEILDDSESPNFDSEDVDETIECISSKLLNSMSTDVAKLKAKQALDSIPKNKLTLLINYAMRNVYLARNYFAGTEDEDEFVDDEVIEKLLNAMDACLLICNIYSTVSDLQFLQEDNVSHIIKFTQFQLRETIFPLHDPVYTAKSIKRTTHRKKIKSHQAQNRSMQLFYLKTVELLKVFVTLFDKCVFVDTIVLPLSTLAIEPFFVDNIETLQFVCLELVTTIFRKERYDKIRNSILGDILTSIDRLPSSKKNLRPYKLTNNGGNIQMVTALVLQLIQCATILPDSLCDNGKFSNKPQEGNTFDEEGKKLLQPSQDLLVLQKYDVAVSIGGNFLTTFLNKCKSRSNETDFRPLFENFIHDLLATVNKPEWPASELLLSLLGTMLVRYVSDKGIEQSIRLVSLDYLGIVAARLRKDTVESRCRVNIIDSMIQSIKLEQEKEGDVTSNNDQFDLEPEEQRTDFLQKILLDFLAVNAQEENLIWDYARHFYLAQWYRDVIYQRRRINDGKKGLAFRKSKIRNNRRTNGDYLDTSDSGSCDESDTDTNKKRIHCVDSNDFELNINIYKALEARKQYFINKIKPFSVFGEQNHSSNQHIKTYIDYNNAQLIAQYLATKRPFSQSFDGCLKKIILVVNEPSIAVRTRAMKCLANIVEVDPLVLKRKDMQMGVNQKFLDTAISVREAAVDLVGKFVLSNQDLIDQYYDMLSTRILDTGVSVRKRVIKILRDICLEYPDFSKIPEICVKMIRRVHDEEGIQKLVTEVFMKMWFTPCTKNDKIGIQRKINHIIDVVNTAHDTGTTWLEGLLMSIFKPRDNMLRSEGCVQEFIKKNSEPPMDIVIACQQLADGLVDRLIELEDTDNSRMLGCITTLHLLAKVRPQLLVKHAITIEPYLNIKCHSATAAKFICAVADILEKVVPLVNNASESFLASLEEHLMLLVVSRNQAEVTSCVSCLGALVNKITHNFKLIRDCFQKFYRVLDVSRSQVIQGNNSVDNIYTPSFRRSLFTIGILMRYFDFKSPIALGETNDGLPVSICEDVFHCLMFFCRCTNQEIRKQALISLGSFCVLNDGYLTRSELKNLYCEILSSIANDAGFKIICMRNIWIYLTESEMFMHNKEKEWEKQSKHEDLKEMNDVSSGMASRIIQLYLEEILECFLNRDDTVRLWAVKVIQIVLRQGLVHPVRMVPYLICLSTDHRIESAHRADALLKDIDKTYSGFVNMKVQFGLQLCFKLQKILQINNRGKLEIIRGYASRGPDNTTTALNDFLYTLLRTTKPQRRALVQTVTKQFDDQKTSLQQMLYIADNLAYFPYVVQDEPLYLIHQIDLLISMAGTHLLATFKEHIKPSDKEGDVLEDDDDVEDPEVLFNRLPEDLTEIIKCITSAQACMLLLILKDHLKEMYAITDSKISRYSPSEQKLYEKAVTRKSVNDFNPKTTIDVIKKQMSQEKLSTDINFTLTKEEKLDLVVKYLDFKQLMLKLDPDDGDSDADESRDKTMLNISASSDGVAFSSSAKNSHSACDGYSLTVTDVVDVPMSHIAKASMLTSKPSGRKTNPVRTKKKRRKIDSTDDETSDAEYA

Target ORF information:

RefSeq Version	NM_001042986.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nipped-B, transcript variant F (Nipped-B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042986.3

ORF Insert Sequence:

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGGTATGTAT AAATCGCCTT AGTATTGAGG ATTCTCGCCT TATGCAACAG 
AGTATTAAAA AGTTTGTACA AAAGTCACCA GAATTGGCTA GAAGCATGGG GTTACTACAA 
GAAACGTGCC AGCAGCAACA TGAAAACTTG AATATTATAC CCACGAGTGC GGAAGAATGT 
GTATTAGAGA GTCGTGCTTC CAGTACAAAT AATACAGTCA AAATCCCTAT TGATACATTT 
TCTACTATAA AAGCCGATGA AAAACGATCC GCGAAAAGAA AACTTGCCAT ATCAATTGGT 
GACATCCCAC CGGAACAAAT ATTTTCAAAG CCTAAAATGC GTCGGGTCGA AAGAATTACA 
CCTCTTTCGA ACACAAAGTG TGTGAAGGAG GAAGTCACCC GTTCGCAAAC ATATCAACAA 
TTTATTCGAA ACATGGATCA CATAATTGAA ATATTAGACG ATAGTGAATC TCCTAACTTT 
GACTCAGAGG ATGTAGACGA AACCATTGAA TGCATTTCCT CAAAACTTTT AAATTCAATG 
AGTACTGATG TTGCCAAGTT AAAAGCTAAG CAGGCTTTAG ATTCAATACC AAAAAATAAG 
TTAACCCTAC TTATTAACTA TGCAATGCGA AACGTGTACT TAGCCCGGAA TTATTTTGCT 
GGAACGGAGG ACGAAGATGA ATTTGTAGAT GATGAAGTTA TTGAAAAATT ACTTAACGCA 
ATGGATGCTT GCTTGCTTAT ATGTAACATT TATTCTACAG TAAGCGACCT TCAGTTCTTA 
CAAGAAGATA ACGTATCACA CATTATAAAA TTTACGCAAT TTCAACTGCG TGAAACAATT 
TTTCCTCTTC ATGACCCGGT TTACACGGCA AAAAGTATAA AAAGGACAAC TCATCGTAAA 
AAAATAAAAT CACACCAAGC TCAAAATCGA AGTATGCAGC TATTCTACTT GAAAACTGTA 
GAGCTATTAA AAGTTTTTGT TACACTTTTT GACAAATGTG TATTTGTTGA TACTATAGTT 
TTACCATTGT CGACACTGGC TATTGAACCA TTTTTTGTTG ACAACATAGA GACGTTGCAA 
TTTGTATGTT TGGAACTCGT TACCACAATT TTTCGCAAGG AAAGATATGA TAAAATAAGA 
AACAGTATCT TAGGCGACAT TTTGACATCT ATTGATCGGT TACCTTCATC AAAAAAAAAT 
CTCCGCCCAT ATAAACTCAC AAACAATGGC GGAAATATTC AAATGGTTAC AGCACTTGTA 
TTGCAATTGA TTCAATGCGC CACGATTTTA CCAGATTCCC TTTGTGATAA TGGCAAATTT 
AGTAACAAAC CCCAAGAGGG AAATACTTTT GATGAAGAAG GAAAAAAATT GCTTCAGCCT 
AGTCAGGATC TACTCGTTTT ACAAAAATAT GATGTAGCTG TCAGCATTGG TGGAAATTTT 
TTAACAACTT TCCTTAACAA ATGCAAATCT CGGTCTAATG AAACTGATTT TCGACCTCTG 
TTTGAAAATT TTATTCATGA TTTACTGGCC ACCGTGAATA AGCCTGAATG GCCTGCATCT 
GAATTGTTGC TTTCGCTGTT GGGAACTATG CTGGTTCGCT ACGTTTCTGA TAAGGGTATA 
GAACAAAGCA TTCGCTTGGT ATCATTGGAT TACCTGGGCA TAGTAGCTGC TCGATTGCGA 
AAAGACACAG TGGAATCTCG GTGTAGAGTT AACATTATTG ATTCAATGAT TCAATCCATC 
AAATTAGAGC AAGAAAAGGA GGGAGATGTA ACATCAAACA ATGATCAATT TGATCTAGAA 
CCCGAGGAGC AGCGAACGGA TTTTTTGCAA AAGATTCTTC TCGACTTTCT AGCTGTAAAT 
GCTCAAGAAG AAAACTTAAT CTGGGACTAT GCTAGACATT TTTATTTGGC ACAATGGTAT 
CGGGATGTCA TATATCAAAG ACGTCGGATA AATGATGGCA AAAAAGGTTT GGCTTTCAGG 
AAGTCCAAAA TTCGTAATAA TCGGAGGACT AATGGTGATT ATTTAGATAC GTCGGATTCT 
GGATCGTGCG ATGAAAGTGA TACCGATACA AACAAAAAGA GAATTCACTG TGTGGACAGC 
AATGATTTCG AACTAAATAT TAACATCTAT AAGGCTTTAG AAGCAAGAAA GCAATATTTT 
ATCAACAAAA TTAAGCCATT TTCAGTTTTT GGCGAGCAAA ACCACTCGTC AAATCAACAC 
ATCAAAACAT ACATAGATTA TAATAACGCA CAGTTAATAG CACAATATCT GGCCACCAAA 
AGGCCCTTCA GTCAATCTTT TGATGGATGC TTAAAGAAAA TTATATTAGT TGTTAATGAG 
CCATCTATAG CAGTAAGGAC ACGTGCAATG AAATGTCTTG CCAACATAGT GGAAGTTGAT 
CCATTGGTTC TAAAACGAAA AGATATGCAA ATGGGAGTTA ATCAGAAATT CCTAGACACA 
GCTATTTCGG TACGAGAAGC TGCAGTAGAT TTGGTTGGAA AATTTGTTCT AAGTAATCAA 
GATCTAATCG ATCAGTATTA CGATATGCTG TCTACTCGTA TTTTGGATAC TGGTGTCTCC 
GTACGGAAAA GGGTCATTAA AATTTTGAGA GATATTTGTT TAGAATATCC AGACTTTTCA 
AAAATTCCCG AAATTTGCGT TAAAATGATT CGACGTGTTC ATGACGAAGA AGGCATACAA 
AAACTTGTCA CTGAAGTTTT TATGAAGATG TGGTTTACAC CTTGTACTAA AAACGACAAA 
ATTGGAATAC AAAGGAAAAT AAATCATATA ATTGACGTGG TAAATACTGC ACATGATACT 
GGAACTACTT GGTTAGAGGG CCTTCTAATG AGTATATTTA AGCCAAGAGA TAATATGCTA 
AGGTCTGAAG GATGCGTTCA AGAATTTATT AAGAAGAACT CAGAGCCACC AATGGATATT 
GTGATAGCTT GTCAACAATT AGCCGACGGA CTAGTTGACA GACTAATTGA ATTGGAAGAT 
ACAGATAACT CGCGAATGCT GGGTTGTATT ACCACACTTC ATTTACTGGC TAAAGTGCGG 
CCCCAACTGC TTGTTAAGCA TGCTATAACA ATTGAACCAT ATCTCAACAT AAAATGTCAT 
TCTGCCACAG CCGCCAAGTT CATATGTGCA GTTGCAGATA TTCTCGAAAA AGTAGTTCCT 
CTGGTTAATA ATGCCAGTGA ATCCTTTTTA GCATCGTTGG AAGAGCATTT AATGTTATTG 
GTTGTTTCAC GAAATCAAGC CGAAGTTACT AGCTGTGTTT CTTGCTTGGG AGCACTTGTG 
AATAAGATTA CACATAACTT TAAATTGATC CGCGACTGTT TTCAAAAATT TTATCGGGTT 
CTTGATGTGT CTCGAAGTCA AGTAATACAA GGAAATAACA GCGTAGATAA TATATACACT 
CCCAGCTTTC GGCGAAGTTT GTTTACGATC GGAATACTAA TGCGATATTT CGACTTTAAG 
TCTCCTATTG CGTTAGGTGA AACAAATGAT GGACTTCCTG TTTCTATATG TGAGGATGTA 
TTCCATTGCT TGATGTTCTT CTGCCGTTGT ACTAATCAAG AAATTCGAAA GCAAGCACTT 
ATTTCGCTTG GATCATTTTG TGTACTAAAC GATGGCTACC TTACAAGATC CGAACTTAAA 
AATTTGTATT GCGAAATTTT AAGTTCAATA GCCAACGACG CCGGCTTTAA AATAATCTGC 
ATGCGAAATA TTTGGATTTA TCTAACGGAA TCTGAAATGT TTATGCACAA TAAGGAAAAA 
GAATGGGAAA AACAATCAAA ACACGAAGAT CTTAAGGAAA TGAACGACGT TTCGTCTGGC 
ATGGCAAGTC GAATAATTCA ACTATATTTA GAAGAAATTC TTGAATGCTT TCTTAATCGG 
GATGACACAG TTCGGTTATG GGCTGTCAAA GTAATACAGA TTGTCCTCCG TCAAGGTTTG 
GTTCACCCTG TTAGAATGGT TCCGTATTTA ATTTGCCTGA GTACTGATCA TAGGATAGAG 
TCTGCTCATC GAGCTGATGC CCTGTTGAAA GATATTGATA AAACATACTC TGGATTTGTT 
AATATGAAAG TGCAATTCGG GCTACAGCTT TGCTTTAAGC TACAAAAAAT TTTACAAATA 
AACAACAGAG GGAAACTGGA AATAATTAGA GGTTATGCGA GTCGTGGACC AGATAATACA 
ACTACCGCTT TAAACGATTT TCTGTATACA CTTCTTCGCA CTACTAAACC GCAAAGGCGT 
GCTTTGGTGC AAACAGTTAC AAAACAGTTT GACGATCAGA AAACATCATT ACAACAAATG 
TTATATATTG CAGATAACCT GGCTTACTTT CCATATGTAG TTCAAGATGA ACCTTTATAT 
CTAATCCATC AAATAGATCT TCTAATTTCT ATGGCTGGAA CACATTTACT TGCTACATTC 
AAGGAACATA TAAAACCAAG CGACAAGGAA GGAGATGTTT TAGAAGATGA TGATGACGTA 
GAAGATCCAG AGGTGTTGTT TAATAGATTG CCCGAAGATT TGACGGAAAT TATTAAATGT 
ATAACATCTG CTCAAGCATG TATGCTTCTT TTAATACTGA AAGATCATCT GAAGGAAATG 
TATGCGATTA CTGACAGTAA AATTTCAAGA TATTCACCTT CCGAACAAAA ATTATATGAA 
AAAGCCGTAA CACGTAAAAG TGTTAATGAT TTTAACCCAA AAACGACTAT TGATGTTATA 
AAGAAACAAA TGTCGCAAGA GAAATTGAGT ACGGACATTA ATTTTACACT GACTAAAGAG 
GAGAAATTGG ACCTTGTTGT TAAATACCTT GATTTTAAGC AACTGATGCT TAAATTGGAT 
CCGGATGATG GAGACTCCGA TGCTGATGAA TCCCGCGACA AAACAATGTT AAATATCTCT 
GCTTCTTCGG ATGGAGTGGC ATTCTCTAGT TCAGCAAAAA ATTCTCACTC CGCTTGTGAT 
GGATACTCAC TAACAGTCAC GGATGTGGTT GATGTCCCAA TGTCTCATAT TGCGAAGGCA 
TCTATGTTGA CTTCAAAACC TAGTGGTCGC AAAACAAATC CTGTGCGAAC TAAAAAAAAA 
AGAAGAAAAA TTGATTCTAC GGATGATGAA ACCAGTGATG CGGAATATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipped-B ( NM_001042987.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036452.1 Drosophila melanogaster Nipped-B, transcript variant E (Nipped-B), mRNA.

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CloneID	OFa11829
Clone ID Related Accession (Same CDS sequence)	NM_001042986.3 , NM_001042987.3
Accession Version	NM_001042987.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nipped-B, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001042987.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGGTATGTAT AAATCGCCTT AGTATTGAGG ATTCTCGCCT TATGCAACAG 
AGTATTAAAA AGTTTGTACA AAAGTCACCA GAATTGGCTA GAAGCATGGG GTTACTACAA 
GAAACGTGCC AGCAGCAACA TGAAAACTTG AATATTATAC CCACGAGTGC GGAAGAATGT 
GTATTAGAGA GTCGTGCTTC CAGTACAAAT AATACAGTCA AAATCCCTAT TGATACATTT 
TCTACTATAA AAGCCGATGA AAAACGATCC GCGAAAAGAA AACTTGCCAT ATCAATTGGT 
GACATCCCAC CGGAACAAAT ATTTTCAAAG CCTAAAATGC GTCGGGTCGA AAGAATTACA 
CCTCTTTCGA ACACAAAGTG TGTGAAGGAG GAAGTCACCC GTTCGCAAAC ATATCAACAA 
TTTATTCGAA ACATGGATCA CATAATTGAA ATATTAGACG ATAGTGAATC TCCTAACTTT 
GACTCAGAGG ATGTAGACGA AACCATTGAA TGCATTTCCT CAAAACTTTT AAATTCAATG 
AGTACTGATG TTGCCAAGTT AAAAGCTAAG CAGGCTTTAG ATTCAATACC AAAAAATAAG 
TTAACCCTAC TTATTAACTA TGCAATGCGA AACGTGTACT TAGCCCGGAA TTATTTTGCT 
GGAACGGAGG ACGAAGATGA ATTTGTAGAT GATGAAGTTA TTGAAAAATT ACTTAACGCA 
ATGGATGCTT GCTTGCTTAT ATGTAACATT TATTCTACAG TAAGCGACCT TCAGTTCTTA 
CAAGAAGATA ACGTATCACA CATTATAAAA TTTACGCAAT TTCAACTGCG TGAAACAATT 
TTTCCTCTTC ATGACCCGGT TTACACGGCA AAAAGTATAA AAAGGACAAC TCATCGTAAA 
AAAATAAAAT CACACCAAGC TCAAAATCGA AGTATGCAGC TATTCTACTT GAAAACTGTA 
GAGCTATTAA AAGTTTTTGT TACACTTTTT GACAAATGTG TATTTGTTGA TACTATAGTT 
TTACCATTGT CGACACTGGC TATTGAACCA TTTTTTGTTG ACAACATAGA GACGTTGCAA 
TTTGTATGTT TGGAACTCGT TACCACAATT TTTCGCAAGG AAAGATATGA TAAAATAAGA 
AACAGTATCT TAGGCGACAT TTTGACATCT ATTGATCGGT TACCTTCATC AAAAAAAAAT 
CTCCGCCCAT ATAAACTCAC AAACAATGGC GGAAATATTC AAATGGTTAC AGCACTTGTA 
TTGCAATTGA TTCAATGCGC CACGATTTTA CCAGATTCCC TTTGTGATAA TGGCAAATTT 
AGTAACAAAC CCCAAGAGGG AAATACTTTT GATGAAGAAG GAAAAAAATT GCTTCAGCCT 
AGTCAGGATC TACTCGTTTT ACAAAAATAT GATGTAGCTG TCAGCATTGG TGGAAATTTT 
TTAACAACTT TCCTTAACAA ATGCAAATCT CGGTCTAATG AAACTGATTT TCGACCTCTG 
TTTGAAAATT TTATTCATGA TTTACTGGCC ACCGTGAATA AGCCTGAATG GCCTGCATCT 
GAATTGTTGC TTTCGCTGTT GGGAACTATG CTGGTTCGCT ACGTTTCTGA TAAGGGTATA 
GAACAAAGCA TTCGCTTGGT ATCATTGGAT TACCTGGGCA TAGTAGCTGC TCGATTGCGA 
AAAGACACAG TGGAATCTCG GTGTAGAGTT AACATTATTG ATTCAATGAT TCAATCCATC 
AAATTAGAGC AAGAAAAGGA GGGAGATGTA ACATCAAACA ATGATCAATT TGATCTAGAA 
CCCGAGGAGC AGCGAACGGA TTTTTTGCAA AAGATTCTTC TCGACTTTCT AGCTGTAAAT 
GCTCAAGAAG AAAACTTAAT CTGGGACTAT GCTAGACATT TTTATTTGGC ACAATGGTAT 
CGGGATGTCA TATATCAAAG ACGTCGGATA AATGATGGCA AAAAAGGTTT GGCTTTCAGG 
AAGTCCAAAA TTCGTAATAA TCGGAGGACT AATGGTGATT ATTTAGATAC GTCGGATTCT 
GGATCGTGCG ATGAAAGTGA TACCGATACA AACAAAAAGA GAATTCACTG TGTGGACAGC 
AATGATTTCG AACTAAATAT TAACATCTAT AAGGCTTTAG AAGCAAGAAA GCAATATTTT 
ATCAACAAAA TTAAGCCATT TTCAGTTTTT GGCGAGCAAA ACCACTCGTC AAATCAACAC 
ATCAAAACAT ACATAGATTA TAATAACGCA CAGTTAATAG CACAATATCT GGCCACCAAA 
AGGCCCTTCA GTCAATCTTT TGATGGATGC TTAAAGAAAA TTATATTAGT TGTTAATGAG 
CCATCTATAG CAGTAAGGAC ACGTGCAATG AAATGTCTTG CCAACATAGT GGAAGTTGAT 
CCATTGGTTC TAAAACGAAA AGATATGCAA ATGGGAGTTA ATCAGAAATT CCTAGACACA 
GCTATTTCGG TACGAGAAGC TGCAGTAGAT TTGGTTGGAA AATTTGTTCT AAGTAATCAA 
GATCTAATCG ATCAGTATTA CGATATGCTG TCTACTCGTA TTTTGGATAC TGGTGTCTCC 
GTACGGAAAA GGGTCATTAA AATTTTGAGA GATATTTGTT TAGAATATCC AGACTTTTCA 
AAAATTCCCG AAATTTGCGT TAAAATGATT CGACGTGTTC ATGACGAAGA AGGCATACAA 
AAACTTGTCA CTGAAGTTTT TATGAAGATG TGGTTTACAC CTTGTACTAA AAACGACAAA 
ATTGGAATAC AAAGGAAAAT AAATCATATA ATTGACGTGG TAAATACTGC ACATGATACT 
GGAACTACTT GGTTAGAGGG CCTTCTAATG AGTATATTTA AGCCAAGAGA TAATATGCTA 
AGGTCTGAAG GATGCGTTCA AGAATTTATT AAGAAGAACT CAGAGCCACC AATGGATATT 
GTGATAGCTT GTCAACAATT AGCCGACGGA CTAGTTGACA GACTAATTGA ATTGGAAGAT 
ACAGATAACT CGCGAATGCT GGGTTGTATT ACCACACTTC ATTTACTGGC TAAAGTGCGG 
CCCCAACTGC TTGTTAAGCA TGCTATAACA ATTGAACCAT ATCTCAACAT AAAATGTCAT 
TCTGCCACAG CCGCCAAGTT CATATGTGCA GTTGCAGATA TTCTCGAAAA AGTAGTTCCT 
CTGGTTAATA ATGCCAGTGA ATCCTTTTTA GCATCGTTGG AAGAGCATTT AATGTTATTG 
GTTGTTTCAC GAAATCAAGC CGAAGTTACT AGCTGTGTTT CTTGCTTGGG AGCACTTGTG 
AATAAGATTA CACATAACTT TAAATTGATC CGCGACTGTT TTCAAAAATT TTATCGGGTT 
CTTGATGTGT CTCGAAGTCA AGTAATACAA GGAAATAACA GCGTAGATAA TATATACACT 
CCCAGCTTTC GGCGAAGTTT GTTTACGATC GGAATACTAA TGCGATATTT CGACTTTAAG 
TCTCCTATTG CGTTAGGTGA AACAAATGAT GGACTTCCTG TTTCTATATG TGAGGATGTA 
TTCCATTGCT TGATGTTCTT CTGCCGTTGT ACTAATCAAG AAATTCGAAA GCAAGCACTT 
ATTTCGCTTG GATCATTTTG TGTACTAAAC GATGGCTACC TTACAAGATC CGAACTTAAA 
AATTTGTATT GCGAAATTTT AAGTTCAATA GCCAACGACG CCGGCTTTAA AATAATCTGC 
ATGCGAAATA TTTGGATTTA TCTAACGGAA TCTGAAATGT TTATGCACAA TAAGGAAAAA 
GAATGGGAAA AACAATCAAA ACACGAAGAT CTTAAGGAAA TGAACGACGT TTCGTCTGGC 
ATGGCAAGTC GAATAATTCA ACTATATTTA GAAGAAATTC TTGAATGCTT TCTTAATCGG 
GATGACACAG TTCGGTTATG GGCTGTCAAA GTAATACAGA TTGTCCTCCG TCAAGGTTTG 
GTTCACCCTG TTAGAATGGT TCCGTATTTA ATTTGCCTGA GTACTGATCA TAGGATAGAG 
TCTGCTCATC GAGCTGATGC CCTGTTGAAA GATATTGATA AAACATACTC TGGATTTGTT 
AATATGAAAG TGCAATTCGG GCTACAGCTT TGCTTTAAGC TACAAAAAAT TTTACAAATA 
AACAACAGAG GGAAACTGGA AATAATTAGA GGTTATGCGA GTCGTGGACC AGATAATACA 
ACTACCGCTT TAAACGATTT TCTGTATACA CTTCTTCGCA CTACTAAACC GCAAAGGCGT 
GCTTTGGTGC AAACAGTTAC AAAACAGTTT GACGATCAGA AAACATCATT ACAACAAATG 
TTATATATTG CAGATAACCT GGCTTACTTT CCATATGTAG TTCAAGATGA ACCTTTATAT 
CTAATCCATC AAATAGATCT TCTAATTTCT ATGGCTGGAA CACATTTACT TGCTACATTC 
AAGGAACATA TAAAACCAAG CGACAAGGAA GGAGATGTTT TAGAAGATGA TGATGACGTA 
GAAGATCCAG AGGTGTTGTT TAATAGATTG CCCGAAGATT TGACGGAAAT TATTAAATGT 
ATAACATCTG CTCAAGCATG TATGCTTCTT TTAATACTGA AAGATCATCT GAAGGAAATG 
TATGCGATTA CTGACAGTAA AATTTCAAGA TATTCACCTT CCGAACAAAA ATTATATGAA 
AAAGCCGTAA CACGTAAAAG TGTTAATGAT TTTAACCCAA AAACGACTAT TGATGTTATA 
AAGAAACAAA TGTCGCAAGA GAAATTGAGT ACGGACATTA ATTTTACACT GACTAAAGAG 
GAGAAATTGG ACCTTGTTGT TAAATACCTT GATTTTAAGC AACTGATGCT TAAATTGGAT 
CCGGATGATG GAGACTCCGA TGCTGATGAA TCCCGCGACA AAACAATGTT AAATATCTCT 
GCTTCTTCGG ATGGAGTGGC ATTCTCTAGT TCAGCAAAAA ATTCTCACTC CGCTTGTGAT 
GGATACTCAC TAACAGTCAC GGATGTGGTT GATGTCCCAA TGTCTCATAT TGCGAAGGCA 
TCTATGTTGA CTTCAAAACC TAGTGGTCGC AAAACAAATC CTGTGCGAAC TAAAAAAAAA 
AGAAGAAAAA TTGATTCTAC GGATGATGAA ACCAGTGATG CGGAATATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036452.1
CDS	292..6525
Translation	MGERDNPTVPVTTLAGLTSTSDLLSELPVADSLQSAASLNKSLLFHALVANESSNLLSMRNENLVRQLVTAIERTNSDNIELIHCPVQDTATNCSTYPELLQGIYHFRPAVFNTSIKIHSPDQHTANARLEAKKMQIDSYSIPECYASPTNLSISNEHQLQDAQACFNQLTSMTSKEILEEFSQINFKENATEKDKIDVIENSDLNVTKILSQNTLKKKSNTPERSTVQSIQEQFFIQQQEANYNLKHDLNQVSTNVGQIQNISDTFPQEPSNFNLNSVNIPNMLYVQSPPFTESEPTQAHHSSIEYLKKKKSQILDVSILNRQQVLENTSLHIINKSSTYQTNQNVSTTTSTSTSSSGKSQVRVCINRLSIEDSRLMQQSIKKFVQKSPELARSMGLLQETCQQQHENLNIIPTSAEECVLESRASSTNNTVKIPIDTFSTIKADEKRSAKRKLAISIGDIPPEQIFSKPKMRRVERITPLSNTKCVKEEVTRSQTYQQFIRNMDHIIEILDDSESPNFDSEDVDETIECISSKLLNSMSTDVAKLKAKQALDSIPKNKLTLLINYAMRNVYLARNYFAGTEDEDEFVDDEVIEKLLNAMDACLLICNIYSTVSDLQFLQEDNVSHIIKFTQFQLRETIFPLHDPVYTAKSIKRTTHRKKIKSHQAQNRSMQLFYLKTVELLKVFVTLFDKCVFVDTIVLPLSTLAIEPFFVDNIETLQFVCLELVTTIFRKERYDKIRNSILGDILTSIDRLPSSKKNLRPYKLTNNGGNIQMVTALVLQLIQCATILPDSLCDNGKFSNKPQEGNTFDEEGKKLLQPSQDLLVLQKYDVAVSIGGNFLTTFLNKCKSRSNETDFRPLFENFIHDLLATVNKPEWPASELLLSLLGTMLVRYVSDKGIEQSIRLVSLDYLGIVAARLRKDTVESRCRVNIIDSMIQSIKLEQEKEGDVTSNNDQFDLEPEEQRTDFLQKILLDFLAVNAQEENLIWDYARHFYLAQWYRDVIYQRRRINDGKKGLAFRKSKIRNNRRTNGDYLDTSDSGSCDESDTDTNKKRIHCVDSNDFELNINIYKALEARKQYFINKIKPFSVFGEQNHSSNQHIKTYIDYNNAQLIAQYLATKRPFSQSFDGCLKKIILVVNEPSIAVRTRAMKCLANIVEVDPLVLKRKDMQMGVNQKFLDTAISVREAAVDLVGKFVLSNQDLIDQYYDMLSTRILDTGVSVRKRVIKILRDICLEYPDFSKIPEICVKMIRRVHDEEGIQKLVTEVFMKMWFTPCTKNDKIGIQRKINHIIDVVNTAHDTGTTWLEGLLMSIFKPRDNMLRSEGCVQEFIKKNSEPPMDIVIACQQLADGLVDRLIELEDTDNSRMLGCITTLHLLAKVRPQLLVKHAITIEPYLNIKCHSATAAKFICAVADILEKVVPLVNNASESFLASLEEHLMLLVVSRNQAEVTSCVSCLGALVNKITHNFKLIRDCFQKFYRVLDVSRSQVIQGNNSVDNIYTPSFRRSLFTIGILMRYFDFKSPIALGETNDGLPVSICEDVFHCLMFFCRCTNQEIRKQALISLGSFCVLNDGYLTRSELKNLYCEILSSIANDAGFKIICMRNIWIYLTESEMFMHNKEKEWEKQSKHEDLKEMNDVSSGMASRIIQLYLEEILECFLNRDDTVRLWAVKVIQIVLRQGLVHPVRMVPYLICLSTDHRIESAHRADALLKDIDKTYSGFVNMKVQFGLQLCFKLQKILQINNRGKLEIIRGYASRGPDNTTTALNDFLYTLLRTTKPQRRALVQTVTKQFDDQKTSLQQMLYIADNLAYFPYVVQDEPLYLIHQIDLLISMAGTHLLATFKEHIKPSDKEGDVLEDDDDVEDPEVLFNRLPEDLTEIIKCITSAQACMLLLILKDHLKEMYAITDSKISRYSPSEQKLYEKAVTRKSVNDFNPKTTIDVIKKQMSQEKLSTDINFTLTKEEKLDLVVKYLDFKQLMLKLDPDDGDSDADESRDKTMLNISASSDGVAFSSSAKNSHSACDGYSLTVTDVVDVPMSHIAKASMLTSKPSGRKTNPVRTKKKRRKIDSTDDETSDAEYA

Target ORF information:

RefSeq Version	NM_001042987.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nipped-B, transcript variant E (Nipped-B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042987.3

ORF Insert Sequence:

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGGTATGTAT AAATCGCCTT AGTATTGAGG ATTCTCGCCT TATGCAACAG 
AGTATTAAAA AGTTTGTACA AAAGTCACCA GAATTGGCTA GAAGCATGGG GTTACTACAA 
GAAACGTGCC AGCAGCAACA TGAAAACTTG AATATTATAC CCACGAGTGC GGAAGAATGT 
GTATTAGAGA GTCGTGCTTC CAGTACAAAT AATACAGTCA AAATCCCTAT TGATACATTT 
TCTACTATAA AAGCCGATGA AAAACGATCC GCGAAAAGAA AACTTGCCAT ATCAATTGGT 
GACATCCCAC CGGAACAAAT ATTTTCAAAG CCTAAAATGC GTCGGGTCGA AAGAATTACA 
CCTCTTTCGA ACACAAAGTG TGTGAAGGAG GAAGTCACCC GTTCGCAAAC ATATCAACAA 
TTTATTCGAA ACATGGATCA CATAATTGAA ATATTAGACG ATAGTGAATC TCCTAACTTT 
GACTCAGAGG ATGTAGACGA AACCATTGAA TGCATTTCCT CAAAACTTTT AAATTCAATG 
AGTACTGATG TTGCCAAGTT AAAAGCTAAG CAGGCTTTAG ATTCAATACC AAAAAATAAG 
TTAACCCTAC TTATTAACTA TGCAATGCGA AACGTGTACT TAGCCCGGAA TTATTTTGCT 
GGAACGGAGG ACGAAGATGA ATTTGTAGAT GATGAAGTTA TTGAAAAATT ACTTAACGCA 
ATGGATGCTT GCTTGCTTAT ATGTAACATT TATTCTACAG TAAGCGACCT TCAGTTCTTA 
CAAGAAGATA ACGTATCACA CATTATAAAA TTTACGCAAT TTCAACTGCG TGAAACAATT 
TTTCCTCTTC ATGACCCGGT TTACACGGCA AAAAGTATAA AAAGGACAAC TCATCGTAAA 
AAAATAAAAT CACACCAAGC TCAAAATCGA AGTATGCAGC TATTCTACTT GAAAACTGTA 
GAGCTATTAA AAGTTTTTGT TACACTTTTT GACAAATGTG TATTTGTTGA TACTATAGTT 
TTACCATTGT CGACACTGGC TATTGAACCA TTTTTTGTTG ACAACATAGA GACGTTGCAA 
TTTGTATGTT TGGAACTCGT TACCACAATT TTTCGCAAGG AAAGATATGA TAAAATAAGA 
AACAGTATCT TAGGCGACAT TTTGACATCT ATTGATCGGT TACCTTCATC AAAAAAAAAT 
CTCCGCCCAT ATAAACTCAC AAACAATGGC GGAAATATTC AAATGGTTAC AGCACTTGTA 
TTGCAATTGA TTCAATGCGC CACGATTTTA CCAGATTCCC TTTGTGATAA TGGCAAATTT 
AGTAACAAAC CCCAAGAGGG AAATACTTTT GATGAAGAAG GAAAAAAATT GCTTCAGCCT 
AGTCAGGATC TACTCGTTTT ACAAAAATAT GATGTAGCTG TCAGCATTGG TGGAAATTTT 
TTAACAACTT TCCTTAACAA ATGCAAATCT CGGTCTAATG AAACTGATTT TCGACCTCTG 
TTTGAAAATT TTATTCATGA TTTACTGGCC ACCGTGAATA AGCCTGAATG GCCTGCATCT 
GAATTGTTGC TTTCGCTGTT GGGAACTATG CTGGTTCGCT ACGTTTCTGA TAAGGGTATA 
GAACAAAGCA TTCGCTTGGT ATCATTGGAT TACCTGGGCA TAGTAGCTGC TCGATTGCGA 
AAAGACACAG TGGAATCTCG GTGTAGAGTT AACATTATTG ATTCAATGAT TCAATCCATC 
AAATTAGAGC AAGAAAAGGA GGGAGATGTA ACATCAAACA ATGATCAATT TGATCTAGAA 
CCCGAGGAGC AGCGAACGGA TTTTTTGCAA AAGATTCTTC TCGACTTTCT AGCTGTAAAT 
GCTCAAGAAG AAAACTTAAT CTGGGACTAT GCTAGACATT TTTATTTGGC ACAATGGTAT 
CGGGATGTCA TATATCAAAG ACGTCGGATA AATGATGGCA AAAAAGGTTT GGCTTTCAGG 
AAGTCCAAAA TTCGTAATAA TCGGAGGACT AATGGTGATT ATTTAGATAC GTCGGATTCT 
GGATCGTGCG ATGAAAGTGA TACCGATACA AACAAAAAGA GAATTCACTG TGTGGACAGC 
AATGATTTCG AACTAAATAT TAACATCTAT AAGGCTTTAG AAGCAAGAAA GCAATATTTT 
ATCAACAAAA TTAAGCCATT TTCAGTTTTT GGCGAGCAAA ACCACTCGTC AAATCAACAC 
ATCAAAACAT ACATAGATTA TAATAACGCA CAGTTAATAG CACAATATCT GGCCACCAAA 
AGGCCCTTCA GTCAATCTTT TGATGGATGC TTAAAGAAAA TTATATTAGT TGTTAATGAG 
CCATCTATAG CAGTAAGGAC ACGTGCAATG AAATGTCTTG CCAACATAGT GGAAGTTGAT 
CCATTGGTTC TAAAACGAAA AGATATGCAA ATGGGAGTTA ATCAGAAATT CCTAGACACA 
GCTATTTCGG TACGAGAAGC TGCAGTAGAT TTGGTTGGAA AATTTGTTCT AAGTAATCAA 
GATCTAATCG ATCAGTATTA CGATATGCTG TCTACTCGTA TTTTGGATAC TGGTGTCTCC 
GTACGGAAAA GGGTCATTAA AATTTTGAGA GATATTTGTT TAGAATATCC AGACTTTTCA 
AAAATTCCCG AAATTTGCGT TAAAATGATT CGACGTGTTC ATGACGAAGA AGGCATACAA 
AAACTTGTCA CTGAAGTTTT TATGAAGATG TGGTTTACAC CTTGTACTAA AAACGACAAA 
ATTGGAATAC AAAGGAAAAT AAATCATATA ATTGACGTGG TAAATACTGC ACATGATACT 
GGAACTACTT GGTTAGAGGG CCTTCTAATG AGTATATTTA AGCCAAGAGA TAATATGCTA 
AGGTCTGAAG GATGCGTTCA AGAATTTATT AAGAAGAACT CAGAGCCACC AATGGATATT 
GTGATAGCTT GTCAACAATT AGCCGACGGA CTAGTTGACA GACTAATTGA ATTGGAAGAT 
ACAGATAACT CGCGAATGCT GGGTTGTATT ACCACACTTC ATTTACTGGC TAAAGTGCGG 
CCCCAACTGC TTGTTAAGCA TGCTATAACA ATTGAACCAT ATCTCAACAT AAAATGTCAT 
TCTGCCACAG CCGCCAAGTT CATATGTGCA GTTGCAGATA TTCTCGAAAA AGTAGTTCCT 
CTGGTTAATA ATGCCAGTGA ATCCTTTTTA GCATCGTTGG AAGAGCATTT AATGTTATTG 
GTTGTTTCAC GAAATCAAGC CGAAGTTACT AGCTGTGTTT CTTGCTTGGG AGCACTTGTG 
AATAAGATTA CACATAACTT TAAATTGATC CGCGACTGTT TTCAAAAATT TTATCGGGTT 
CTTGATGTGT CTCGAAGTCA AGTAATACAA GGAAATAACA GCGTAGATAA TATATACACT 
CCCAGCTTTC GGCGAAGTTT GTTTACGATC GGAATACTAA TGCGATATTT CGACTTTAAG 
TCTCCTATTG CGTTAGGTGA AACAAATGAT GGACTTCCTG TTTCTATATG TGAGGATGTA 
TTCCATTGCT TGATGTTCTT CTGCCGTTGT ACTAATCAAG AAATTCGAAA GCAAGCACTT 
ATTTCGCTTG GATCATTTTG TGTACTAAAC GATGGCTACC TTACAAGATC CGAACTTAAA 
AATTTGTATT GCGAAATTTT AAGTTCAATA GCCAACGACG CCGGCTTTAA AATAATCTGC 
ATGCGAAATA TTTGGATTTA TCTAACGGAA TCTGAAATGT TTATGCACAA TAAGGAAAAA 
GAATGGGAAA AACAATCAAA ACACGAAGAT CTTAAGGAAA TGAACGACGT TTCGTCTGGC 
ATGGCAAGTC GAATAATTCA ACTATATTTA GAAGAAATTC TTGAATGCTT TCTTAATCGG 
GATGACACAG TTCGGTTATG GGCTGTCAAA GTAATACAGA TTGTCCTCCG TCAAGGTTTG 
GTTCACCCTG TTAGAATGGT TCCGTATTTA ATTTGCCTGA GTACTGATCA TAGGATAGAG 
TCTGCTCATC GAGCTGATGC CCTGTTGAAA GATATTGATA AAACATACTC TGGATTTGTT 
AATATGAAAG TGCAATTCGG GCTACAGCTT TGCTTTAAGC TACAAAAAAT TTTACAAATA 
AACAACAGAG GGAAACTGGA AATAATTAGA GGTTATGCGA GTCGTGGACC AGATAATACA 
ACTACCGCTT TAAACGATTT TCTGTATACA CTTCTTCGCA CTACTAAACC GCAAAGGCGT 
GCTTTGGTGC AAACAGTTAC AAAACAGTTT GACGATCAGA AAACATCATT ACAACAAATG 
TTATATATTG CAGATAACCT GGCTTACTTT CCATATGTAG TTCAAGATGA ACCTTTATAT 
CTAATCCATC AAATAGATCT TCTAATTTCT ATGGCTGGAA CACATTTACT TGCTACATTC 
AAGGAACATA TAAAACCAAG CGACAAGGAA GGAGATGTTT TAGAAGATGA TGATGACGTA 
GAAGATCCAG AGGTGTTGTT TAATAGATTG CCCGAAGATT TGACGGAAAT TATTAAATGT 
ATAACATCTG CTCAAGCATG TATGCTTCTT TTAATACTGA AAGATCATCT GAAGGAAATG 
TATGCGATTA CTGACAGTAA AATTTCAAGA TATTCACCTT CCGAACAAAA ATTATATGAA 
AAAGCCGTAA CACGTAAAAG TGTTAATGAT TTTAACCCAA AAACGACTAT TGATGTTATA 
AAGAAACAAA TGTCGCAAGA GAAATTGAGT ACGGACATTA ATTTTACACT GACTAAAGAG 
GAGAAATTGG ACCTTGTTGT TAAATACCTT GATTTTAAGC AACTGATGCT TAAATTGGAT 
CCGGATGATG GAGACTCCGA TGCTGATGAA TCCCGCGACA AAACAATGTT AAATATCTCT 
GCTTCTTCGG ATGGAGTGGC ATTCTCTAGT TCAGCAAAAA ATTCTCACTC CGCTTGTGAT 
GGATACTCAC TAACAGTCAC GGATGTGGTT GATGTCCCAA TGTCTCATAT TGCGAAGGCA 
TCTATGTTGA CTTCAAAACC TAGTGGTCGC AAAACAAATC CTGTGCGAAC TAAAAAAAAA 
AGAAGAAAAA TTGATTCTAC GGATGATGAA ACCAGTGATG CGGAATATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipped-B ( NM_001169583.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163054.1 Drosophila melanogaster Nipped-B, transcript variant I (Nipped-B), mRNA.

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CloneID	OFa13073
Clone ID Related Accession (Same CDS sequence)	NM_001169583.2 , NM_001169582.2 , NM_001169581.2
Accession Version	NM_001169583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nipped-B, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169583.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGTCACCAGA ATTGGCTAGA AGCATGGGGT TACTACAAGA AACGTGCCAG 
CAGCAACATG AAAACTTGAA TATTATACCC ACGAGTGCGG AAGAATGTGT ATTAGAGAGT 
CGTGCTTCCA GTACAAATAA TACAGTCAAA ATCCCTATTG ATACATTTTC TACTATAAAA 
GCCGATGAAA AACGATCCGC GAAAAGAAAA CTTGCCATAT CAATTGGTGA CATCCCACCG 
GAACAAATAT TTTCAAAGCC TAAAATGCGT CGGGTCGAAA GAATTACACC TCTTTCGAAC 
ACAAAGTGTG TGAAGGAGGA AGTCACCCGT TCGCAAACAT ATCAACAATT TATTCGAAAC 
ATGGATCACA TAATTGAAAT ATTAGACGAT AGTGAATCTC CTAACTTTGA CTCAGAGGAT 
GTAGACGAAA CCATTGAATG CATTTCCTCA AAACTTTTAA ATTCAATGAG TACTGATGTT 
GCCAAGTTAA AAGCTAAGCA GGCTTTAGAT TCAATACCAA AAAATAAGTT AACCCTACTT 
ATTAACTATG CAATGCGAAA CGTGTACTTA GCCCGGAATT ATTTTGCTGG AACGGAGGAC 
GAAGATGAAT TTGTAGATGA TGAAGTTATT GAAAAATTAC TTAACGCAAT GGATGCTTGC 
TTGCTTATAT GTAACATTTA TTCTACAGTA AGCGACCTTC AGTTCTTACA AGAAGATAAC 
GTATCACACA TTATAAAATT TACGCAATTT CAACTGCGTG AAACAATTTT TCCTCTTCAT 
GACCCGGTTT ACACGGCAAA AAGTATAAAA AGGACAACTC ATCGTAAAAA AATAAAATCA 
CACCAAGCTC AAAATCGAAG TATGCAGCTA TTCTACTTGA AAACTGTAGA GCTATTAAAA 
GTTTTTGTTA CACTTTTTGA CAAATGTGTA TTTGTTGATA CTATAGTTTT ACCATTGTCG 
ACACTGGCTA TTGAACCATT TTTTGTTGAC AACATAGAGA CGTTGCAATT TGTATGTTTG 
GAACTCGTTA CCACAATTTT TCGCAAGGAA AGATATGATA AAATAAGAAA CAGTATCTTA 
GGCGACATTT TGACATCTAT TGATCGGTTA CCTTCATCAA AAAAAAATCT CCGCCCATAT 
AAACTCACAA ACAATGGCGG AAATATTCAA ATGGTTACAG CACTTGTATT GCAATTGATT 
CAATGCGCCA CGATTTTACC AGATTCCCTT TGTGATAATG GCAAATTTAG TAACAAACCC 
CAAGAGGGAA ATACTTTTGA TGAAGAAGGA AAAAAATTGC TTCAGCCTAG TCAGGATCTA 
CTCGTTTTAC AAAAATATGA TGTAGCTGTC AGCATTGGTG GAAATTTTTT AACAACTTTC 
CTTAACAAAT GCAAATCTCG GTCTAATGAA ACTGATTTTC GACCTCTGTT TGAAAATTTT 
ATTCATGATT TACTGGCCAC CGTGAATAAG CCTGAATGGC CTGCATCTGA ATTGTTGCTT 
TCGCTGTTGG GAACTATGCT GGTTCGCTAC GTTTCTGATA AGGGTATAGA ACAAAGCATT 
CGCTTGGTAT CATTGGATTA CCTGGGCATA GTAGCTGCTC GATTGCGAAA AGACACAGTG 
GAATCTCGGT GTAGAGTTAA CATTATTGAT TCAATGATTC AATCCATCAA ATTAGAGCAA 
GAAAAGGAGG GAGATGTAAC ATCAAACAAT GATCAATTTG ATCTAGAACC CGAGGAGCAG 
CGAACGGATT TTTTGCAAAA GATTCTTCTC GACTTTCTAG CTGTAAATGC TCAAGAAGAA 
AACTTAATCT GGGACTATGC TAGACATTTT TATTTGGCAC AATGGTATCG GGATGTCATA 
TATCAAAGAC GTCGGATAAA TGATGGCAAA AAAGGTTTGG CTTTCAGGAA GTCCAAAATT 
CGTAATAATC GGAGGACTAA TGGTGATTAT TTAGATACGT CGGATTCTGG ATCGTGCGAT 
GAAAGTGATA CCGATACAAA CAAAAAGAGA ATTCACTGTG TGGACAGCAA TGATTTCGAA 
CTAAATATTA ACATCTATAA GGCTTTAGAA GCAAGAAAGC AATATTTTAT CAACAAAATT 
AAGCCATTTT CAGTTTTTGG CGAGCAAAAC CACTCGTCAA ATCAACACAT CAAAACATAC 
ATAGATTATA ATAACGCACA GTTAATAGCA CAATATCTGG CCACCAAAAG GCCCTTCAGT 
CAATCTTTTG ATGGATGCTT AAAGAAAATT ATATTAGTTG TTAATGAGCC ATCTATAGCA 
GTAAGGACAC GTGCAATGAA ATGTCTTGCC AACATAGTGG AAGTTGATCC ATTGGTTCTA 
AAACGAAAAG ATATGCAAAT GGGAGTTAAT CAGAAATTCC TAGACACAGC TATTTCGGTA 
CGAGAAGCTG CAGTAGATTT GGTTGGAAAA TTTGTTCTAA GTAATCAAGA TCTAATCGAT 
CAGTATTACG ATATGCTGTC TACTCGTATT TTGGATACTG GTGTCTCCGT ACGGAAAAGG 
GTCATTAAAA TTTTGAGAGA TATTTGTTTA GAATATCCAG ACTTTTCAAA AATTCCCGAA 
ATTTGCGTTA AAATGATTCG ACGTGTTCAT GACGAAGAAG GCATACAAAA ACTTGTCACT 
GAAGTTTTTA TGAAGATGTG GTTTACACCT TGTACTAAAA ACGACAAAAT TGGAATACAA 
AGGAAAATAA ATCATATAAT TGACGTGGTA AATACTGCAC ATGATACTGG AACTACTTGG 
TTAGAGGGCC TTCTAATGAG TATATTTAAG CCAAGAGATA ATATGCTAAG GTCTGAAGGA 
TGCGTTCAAG AATTTATTAA GAAGAACTCA GAGCCACCAA TGGATATTGT GATAGCTTGT 
CAACAATTAG CCGACGGACT AGTTGACAGA CTAATTGAAT TGGAAGATAC AGATAACTCG 
CGAATGCTGG GTTGTATTAC CACACTTCAT TTACTGGCTA AAGTGCGGCC CCAACTGCTT 
GTTAAGCATG CTATAACAAT TGAACCATAT CTCAACATAA AATGTCATTC TGCCACAGCC 
GCCAAGTTCA TATGTGCAGT TGCAGATATT CTCGAAAAAG TAGTTCCTCT GGTTAATAAT 
GCCAGTGAAT CCTTTTTAGC ATCGTTGGAA GAGCATTTAA TGTTATTGGT TGTTTCACGA 
AATCAAGCCG AAGTTACTAG CTGTGTTTCT TGCTTGGGAG CACTTGTGAA TAAGATTACA 
CATAACTTTA AATTGATCCG CGACTGTTTT CAAAAATTTT ATCGGGTTCT TGATGTGTCT 
CGAAGTCAAG TAATACAAGG AAATAACAGC GTAGATAATA TATACACTCC CAGCTTTCGG 
CGAAGTTTGT TTACGATCGG AATACTAATG CGATATTTCG ACTTTAAGTC TCCTATTGCG 
TTAGGTGAAA CAAATGATGG ACTTCCTGTT TCTATATGTG AGGATGTATT CCATTGCTTG 
ATGTTCTTCT GCCGTTGTAC TAATCAAGAA ATTCGAAAGC AAGCACTTAT TTCGCTTGGA 
TCATTTTGTG TACTAAACGA TGGCTACCTT ACAAGATCCG AACTTAAAAA TTTGTATTGC 
GAAATTTTAA GTTCAATAGC CAACGACGCC GGCTTTAAAA TAATCTGCAT GCGAAATATT 
TGGATTTATC TAACGGAATC TGAAATGTTT ATGCACAATA AGGAAAAAGA ATGGGAAAAA 
CAATCAAAAC ACGAAGATCT TAAGGAAATG AACGACGTTT CGTCTGGCAT GGCAAGTCGA 
ATAATTCAAC TATATTTAGA AGAAATTCTT GAATGCTTTC TTAATCGGGA TGACACAGTT 
CGGTTATGGG CTGTCAAAGT AATACAGATT GTCCTCCGTC AAGGTTTGGT TCACCCTGTT 
AGAATGGTTC CGTATTTAAT TTGCCTGAGT ACTGATCATA GGATAGAGTC TGCTCATCGA 
GCTGATGCCC TGTTGAAAGA TATTGATAAA ACATACTCTG GATTTGTTAA TATGAAAGTG 
CAATTCGGGC TACAGCTTTG CTTTAAGCTA CAAAAAATTT TACAAATAAA CAACAGAGGG 
AAACTGGAAA TAATTAGAGG TTATGCGAGT CGTGGACCAG ATAATACAAC TACCGCTTTA 
AACGATTTTC TGTATACACT TCTTCGCACT ACTAAACCGC AAAGGCGTGC TTTGGTGCAA 
ACAGTTACAA AACAGTTTGA CGATCAGAAA ACATCATTAC AACAAATGTT ATATATTGCA 
GATAACCTGG CTTACTTTCC ATATGTAGTT CAAGATGAAC CTTTATATCT AATCCATCAA 
ATAGATCTTC TAATTTCTAT GGCTGGAACA CATTTACTTG CTACATTCAA GGAACATATA 
AAACCAAGCG ACAAGGAAGG AGATGTTTTA GAAGATGATG ATGACGTAGA AGATCCAGAG 
GTGTTGTTTA ATAGATTGCC CGAAGATTTG ACGGAAATTA TTAAATGTAT AACATCTGCT 
CAAGCATGTA TGCTTCTTTT AATACTGAAA GATCATCTGA AGGAAATGTA TGCGATTACT 
GACAGTAAAA TTTCAAGATA TTCACCTTCC GAACAAAAAT TATATGAAAA AGCCGTAACA 
CGTAAAAGTG TTAATGATTT TAACCCAAAA ACGACTATTG ATGTTATAAA GAAACAAATG 
TCGCAAGAGA AATTGAGTAC GGACATTAAT TTTACACTGA CTAAAGAGGA GAAATTGGAC 
CTTGTTGTTA AATACCTTGA TTTTAAGCAA CTGATGCTTA AATTGGATCC GGATGATGGA 
GACTCCGATG CTGATGAATC CCGCGACAAA ACAATGTTAA ATATCTCTGC TTCTTCGGAT 
GGAGTGGCAT TCTCTAGTTC AGCAAAAAAT TCTCACTCCG CTTGTGATGG ATACTCACTA 
ACAGTCACGG ATGTGGTTGA TGTCCCAATG TCTCATATTG CGAAGGCATC TATGTTGACT 
TCAAAACCTA GTGGTCGCAA AACAAATCCT GTGCGAACTA AAAAAAAAAG AAGAAAAATT 
GATTCTACGG ATGATGAAAC CAGTGATGCG GAATATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163054.1
CDS	264..6425
Translation	MGERDNPTVPVTTLAGLTSTSDLLSELPVADSLQSAASLNKSLLFHALVANESSNLLSMRNENLVRQLVTAIERTNSDNIELIHCPVQDTATNCSTYPELLQGIYHFRPAVFNTSIKIHSPDQHTANARLEAKKMQIDSYSIPECYASPTNLSISNEHQLQDAQACFNQLTSMTSKEILEEFSQINFKENATEKDKIDVIENSDLNVTKILSQNTLKKKSNTPERSTVQSIQEQFFIQQQEANYNLKHDLNQVSTNVGQIQNISDTFPQEPSNFNLNSVNIPNMLYVQSPPFTESEPTQAHHSSIEYLKKKKSQILDVSILNRQQVLENTSLHIINKSSTYQTNQNVSTTTSTSTSSSGKSQVRSPELARSMGLLQETCQQQHENLNIIPTSAEECVLESRASSTNNTVKIPIDTFSTIKADEKRSAKRKLAISIGDIPPEQIFSKPKMRRVERITPLSNTKCVKEEVTRSQTYQQFIRNMDHIIEILDDSESPNFDSEDVDETIECISSKLLNSMSTDVAKLKAKQALDSIPKNKLTLLINYAMRNVYLARNYFAGTEDEDEFVDDEVIEKLLNAMDACLLICNIYSTVSDLQFLQEDNVSHIIKFTQFQLRETIFPLHDPVYTAKSIKRTTHRKKIKSHQAQNRSMQLFYLKTVELLKVFVTLFDKCVFVDTIVLPLSTLAIEPFFVDNIETLQFVCLELVTTIFRKERYDKIRNSILGDILTSIDRLPSSKKNLRPYKLTNNGGNIQMVTALVLQLIQCATILPDSLCDNGKFSNKPQEGNTFDEEGKKLLQPSQDLLVLQKYDVAVSIGGNFLTTFLNKCKSRSNETDFRPLFENFIHDLLATVNKPEWPASELLLSLLGTMLVRYVSDKGIEQSIRLVSLDYLGIVAARLRKDTVESRCRVNIIDSMIQSIKLEQEKEGDVTSNNDQFDLEPEEQRTDFLQKILLDFLAVNAQEENLIWDYARHFYLAQWYRDVIYQRRRINDGKKGLAFRKSKIRNNRRTNGDYLDTSDSGSCDESDTDTNKKRIHCVDSNDFELNINIYKALEARKQYFINKIKPFSVFGEQNHSSNQHIKTYIDYNNAQLIAQYLATKRPFSQSFDGCLKKIILVVNEPSIAVRTRAMKCLANIVEVDPLVLKRKDMQMGVNQKFLDTAISVREAAVDLVGKFVLSNQDLIDQYYDMLSTRILDTGVSVRKRVIKILRDICLEYPDFSKIPEICVKMIRRVHDEEGIQKLVTEVFMKMWFTPCTKNDKIGIQRKINHIIDVVNTAHDTGTTWLEGLLMSIFKPRDNMLRSEGCVQEFIKKNSEPPMDIVIACQQLADGLVDRLIELEDTDNSRMLGCITTLHLLAKVRPQLLVKHAITIEPYLNIKCHSATAAKFICAVADILEKVVPLVNNASESFLASLEEHLMLLVVSRNQAEVTSCVSCLGALVNKITHNFKLIRDCFQKFYRVLDVSRSQVIQGNNSVDNIYTPSFRRSLFTIGILMRYFDFKSPIALGETNDGLPVSICEDVFHCLMFFCRCTNQEIRKQALISLGSFCVLNDGYLTRSELKNLYCEILSSIANDAGFKIICMRNIWIYLTESEMFMHNKEKEWEKQSKHEDLKEMNDVSSGMASRIIQLYLEEILECFLNRDDTVRLWAVKVIQIVLRQGLVHPVRMVPYLICLSTDHRIESAHRADALLKDIDKTYSGFVNMKVQFGLQLCFKLQKILQINNRGKLEIIRGYASRGPDNTTTALNDFLYTLLRTTKPQRRALVQTVTKQFDDQKTSLQQMLYIADNLAYFPYVVQDEPLYLIHQIDLLISMAGTHLLATFKEHIKPSDKEGDVLEDDDDVEDPEVLFNRLPEDLTEIIKCITSAQACMLLLILKDHLKEMYAITDSKISRYSPSEQKLYEKAVTRKSVNDFNPKTTIDVIKKQMSQEKLSTDINFTLTKEEKLDLVVKYLDFKQLMLKLDPDDGDSDADESRDKTMLNISASSDGVAFSSSAKNSHSACDGYSLTVTDVVDVPMSHIAKASMLTSKPSGRKTNPVRTKKKRRKIDSTDDETSDAEYA

Target ORF information:

RefSeq Version	NM_001169583.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nipped-B, transcript variant I (Nipped-B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169583.2

ORF Insert Sequence:

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGTCACCAGA ATTGGCTAGA AGCATGGGGT TACTACAAGA AACGTGCCAG 
CAGCAACATG AAAACTTGAA TATTATACCC ACGAGTGCGG AAGAATGTGT ATTAGAGAGT 
CGTGCTTCCA GTACAAATAA TACAGTCAAA ATCCCTATTG ATACATTTTC TACTATAAAA 
GCCGATGAAA AACGATCCGC GAAAAGAAAA CTTGCCATAT CAATTGGTGA CATCCCACCG 
GAACAAATAT TTTCAAAGCC TAAAATGCGT CGGGTCGAAA GAATTACACC TCTTTCGAAC 
ACAAAGTGTG TGAAGGAGGA AGTCACCCGT TCGCAAACAT ATCAACAATT TATTCGAAAC 
ATGGATCACA TAATTGAAAT ATTAGACGAT AGTGAATCTC CTAACTTTGA CTCAGAGGAT 
GTAGACGAAA CCATTGAATG CATTTCCTCA AAACTTTTAA ATTCAATGAG TACTGATGTT 
GCCAAGTTAA AAGCTAAGCA GGCTTTAGAT TCAATACCAA AAAATAAGTT AACCCTACTT 
ATTAACTATG CAATGCGAAA CGTGTACTTA GCCCGGAATT ATTTTGCTGG AACGGAGGAC 
GAAGATGAAT TTGTAGATGA TGAAGTTATT GAAAAATTAC TTAACGCAAT GGATGCTTGC 
TTGCTTATAT GTAACATTTA TTCTACAGTA AGCGACCTTC AGTTCTTACA AGAAGATAAC 
GTATCACACA TTATAAAATT TACGCAATTT CAACTGCGTG AAACAATTTT TCCTCTTCAT 
GACCCGGTTT ACACGGCAAA AAGTATAAAA AGGACAACTC ATCGTAAAAA AATAAAATCA 
CACCAAGCTC AAAATCGAAG TATGCAGCTA TTCTACTTGA AAACTGTAGA GCTATTAAAA 
GTTTTTGTTA CACTTTTTGA CAAATGTGTA TTTGTTGATA CTATAGTTTT ACCATTGTCG 
ACACTGGCTA TTGAACCATT TTTTGTTGAC AACATAGAGA CGTTGCAATT TGTATGTTTG 
GAACTCGTTA CCACAATTTT TCGCAAGGAA AGATATGATA AAATAAGAAA CAGTATCTTA 
GGCGACATTT TGACATCTAT TGATCGGTTA CCTTCATCAA AAAAAAATCT CCGCCCATAT 
AAACTCACAA ACAATGGCGG AAATATTCAA ATGGTTACAG CACTTGTATT GCAATTGATT 
CAATGCGCCA CGATTTTACC AGATTCCCTT TGTGATAATG GCAAATTTAG TAACAAACCC 
CAAGAGGGAA ATACTTTTGA TGAAGAAGGA AAAAAATTGC TTCAGCCTAG TCAGGATCTA 
CTCGTTTTAC AAAAATATGA TGTAGCTGTC AGCATTGGTG GAAATTTTTT AACAACTTTC 
CTTAACAAAT GCAAATCTCG GTCTAATGAA ACTGATTTTC GACCTCTGTT TGAAAATTTT 
ATTCATGATT TACTGGCCAC CGTGAATAAG CCTGAATGGC CTGCATCTGA ATTGTTGCTT 
TCGCTGTTGG GAACTATGCT GGTTCGCTAC GTTTCTGATA AGGGTATAGA ACAAAGCATT 
CGCTTGGTAT CATTGGATTA CCTGGGCATA GTAGCTGCTC GATTGCGAAA AGACACAGTG 
GAATCTCGGT GTAGAGTTAA CATTATTGAT TCAATGATTC AATCCATCAA ATTAGAGCAA 
GAAAAGGAGG GAGATGTAAC ATCAAACAAT GATCAATTTG ATCTAGAACC CGAGGAGCAG 
CGAACGGATT TTTTGCAAAA GATTCTTCTC GACTTTCTAG CTGTAAATGC TCAAGAAGAA 
AACTTAATCT GGGACTATGC TAGACATTTT TATTTGGCAC AATGGTATCG GGATGTCATA 
TATCAAAGAC GTCGGATAAA TGATGGCAAA AAAGGTTTGG CTTTCAGGAA GTCCAAAATT 
CGTAATAATC GGAGGACTAA TGGTGATTAT TTAGATACGT CGGATTCTGG ATCGTGCGAT 
GAAAGTGATA CCGATACAAA CAAAAAGAGA ATTCACTGTG TGGACAGCAA TGATTTCGAA 
CTAAATATTA ACATCTATAA GGCTTTAGAA GCAAGAAAGC AATATTTTAT CAACAAAATT 
AAGCCATTTT CAGTTTTTGG CGAGCAAAAC CACTCGTCAA ATCAACACAT CAAAACATAC 
ATAGATTATA ATAACGCACA GTTAATAGCA CAATATCTGG CCACCAAAAG GCCCTTCAGT 
CAATCTTTTG ATGGATGCTT AAAGAAAATT ATATTAGTTG TTAATGAGCC ATCTATAGCA 
GTAAGGACAC GTGCAATGAA ATGTCTTGCC AACATAGTGG AAGTTGATCC ATTGGTTCTA 
AAACGAAAAG ATATGCAAAT GGGAGTTAAT CAGAAATTCC TAGACACAGC TATTTCGGTA 
CGAGAAGCTG CAGTAGATTT GGTTGGAAAA TTTGTTCTAA GTAATCAAGA TCTAATCGAT 
CAGTATTACG ATATGCTGTC TACTCGTATT TTGGATACTG GTGTCTCCGT ACGGAAAAGG 
GTCATTAAAA TTTTGAGAGA TATTTGTTTA GAATATCCAG ACTTTTCAAA AATTCCCGAA 
ATTTGCGTTA AAATGATTCG ACGTGTTCAT GACGAAGAAG GCATACAAAA ACTTGTCACT 
GAAGTTTTTA TGAAGATGTG GTTTACACCT TGTACTAAAA ACGACAAAAT TGGAATACAA 
AGGAAAATAA ATCATATAAT TGACGTGGTA AATACTGCAC ATGATACTGG AACTACTTGG 
TTAGAGGGCC TTCTAATGAG TATATTTAAG CCAAGAGATA ATATGCTAAG GTCTGAAGGA 
TGCGTTCAAG AATTTATTAA GAAGAACTCA GAGCCACCAA TGGATATTGT GATAGCTTGT 
CAACAATTAG CCGACGGACT AGTTGACAGA CTAATTGAAT TGGAAGATAC AGATAACTCG 
CGAATGCTGG GTTGTATTAC CACACTTCAT TTACTGGCTA AAGTGCGGCC CCAACTGCTT 
GTTAAGCATG CTATAACAAT TGAACCATAT CTCAACATAA AATGTCATTC TGCCACAGCC 
GCCAAGTTCA TATGTGCAGT TGCAGATATT CTCGAAAAAG TAGTTCCTCT GGTTAATAAT 
GCCAGTGAAT CCTTTTTAGC ATCGTTGGAA GAGCATTTAA TGTTATTGGT TGTTTCACGA 
AATCAAGCCG AAGTTACTAG CTGTGTTTCT TGCTTGGGAG CACTTGTGAA TAAGATTACA 
CATAACTTTA AATTGATCCG CGACTGTTTT CAAAAATTTT ATCGGGTTCT TGATGTGTCT 
CGAAGTCAAG TAATACAAGG AAATAACAGC GTAGATAATA TATACACTCC CAGCTTTCGG 
CGAAGTTTGT TTACGATCGG AATACTAATG CGATATTTCG ACTTTAAGTC TCCTATTGCG 
TTAGGTGAAA CAAATGATGG ACTTCCTGTT TCTATATGTG AGGATGTATT CCATTGCTTG 
ATGTTCTTCT GCCGTTGTAC TAATCAAGAA ATTCGAAAGC AAGCACTTAT TTCGCTTGGA 
TCATTTTGTG TACTAAACGA TGGCTACCTT ACAAGATCCG AACTTAAAAA TTTGTATTGC 
GAAATTTTAA GTTCAATAGC CAACGACGCC GGCTTTAAAA TAATCTGCAT GCGAAATATT 
TGGATTTATC TAACGGAATC TGAAATGTTT ATGCACAATA AGGAAAAAGA ATGGGAAAAA 
CAATCAAAAC ACGAAGATCT TAAGGAAATG AACGACGTTT CGTCTGGCAT GGCAAGTCGA 
ATAATTCAAC TATATTTAGA AGAAATTCTT GAATGCTTTC TTAATCGGGA TGACACAGTT 
CGGTTATGGG CTGTCAAAGT AATACAGATT GTCCTCCGTC AAGGTTTGGT TCACCCTGTT 
AGAATGGTTC CGTATTTAAT TTGCCTGAGT ACTGATCATA GGATAGAGTC TGCTCATCGA 
GCTGATGCCC TGTTGAAAGA TATTGATAAA ACATACTCTG GATTTGTTAA TATGAAAGTG 
CAATTCGGGC TACAGCTTTG CTTTAAGCTA CAAAAAATTT TACAAATAAA CAACAGAGGG 
AAACTGGAAA TAATTAGAGG TTATGCGAGT CGTGGACCAG ATAATACAAC TACCGCTTTA 
AACGATTTTC TGTATACACT TCTTCGCACT ACTAAACCGC AAAGGCGTGC TTTGGTGCAA 
ACAGTTACAA AACAGTTTGA CGATCAGAAA ACATCATTAC AACAAATGTT ATATATTGCA 
GATAACCTGG CTTACTTTCC ATATGTAGTT CAAGATGAAC CTTTATATCT AATCCATCAA 
ATAGATCTTC TAATTTCTAT GGCTGGAACA CATTTACTTG CTACATTCAA GGAACATATA 
AAACCAAGCG ACAAGGAAGG AGATGTTTTA GAAGATGATG ATGACGTAGA AGATCCAGAG 
GTGTTGTTTA ATAGATTGCC CGAAGATTTG ACGGAAATTA TTAAATGTAT AACATCTGCT 
CAAGCATGTA TGCTTCTTTT AATACTGAAA GATCATCTGA AGGAAATGTA TGCGATTACT 
GACAGTAAAA TTTCAAGATA TTCACCTTCC GAACAAAAAT TATATGAAAA AGCCGTAACA 
CGTAAAAGTG TTAATGATTT TAACCCAAAA ACGACTATTG ATGTTATAAA GAAACAAATG 
TCGCAAGAGA AATTGAGTAC GGACATTAAT TTTACACTGA CTAAAGAGGA GAAATTGGAC 
CTTGTTGTTA AATACCTTGA TTTTAAGCAA CTGATGCTTA AATTGGATCC GGATGATGGA 
GACTCCGATG CTGATGAATC CCGCGACAAA ACAATGTTAA ATATCTCTGC TTCTTCGGAT 
GGAGTGGCAT TCTCTAGTTC AGCAAAAAAT TCTCACTCCG CTTGTGATGG ATACTCACTA 
ACAGTCACGG ATGTGGTTGA TGTCCCAATG TCTCATATTG CGAAGGCATC TATGTTGACT 
TCAAAACCTA GTGGTCGCAA AACAAATCCT GTGCGAACTA AAAAAAAAAG AAGAAAAATT 
GATTCTACGG ATGATGAAAC CAGTGATGCG GAATATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipped-B ( NM_001169582.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163053.1 Drosophila melanogaster Nipped-B, transcript variant H (Nipped-B), mRNA.

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CloneID	OFa13073
Clone ID Related Accession (Same CDS sequence)	NM_001169583.2 , NM_001169582.2 , NM_001169581.2
Accession Version	NM_001169582.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nipped-B, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169582.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGTCACCAGA ATTGGCTAGA AGCATGGGGT TACTACAAGA AACGTGCCAG 
CAGCAACATG AAAACTTGAA TATTATACCC ACGAGTGCGG AAGAATGTGT ATTAGAGAGT 
CGTGCTTCCA GTACAAATAA TACAGTCAAA ATCCCTATTG ATACATTTTC TACTATAAAA 
GCCGATGAAA AACGATCCGC GAAAAGAAAA CTTGCCATAT CAATTGGTGA CATCCCACCG 
GAACAAATAT TTTCAAAGCC TAAAATGCGT CGGGTCGAAA GAATTACACC TCTTTCGAAC 
ACAAAGTGTG TGAAGGAGGA AGTCACCCGT TCGCAAACAT ATCAACAATT TATTCGAAAC 
ATGGATCACA TAATTGAAAT ATTAGACGAT AGTGAATCTC CTAACTTTGA CTCAGAGGAT 
GTAGACGAAA CCATTGAATG CATTTCCTCA AAACTTTTAA ATTCAATGAG TACTGATGTT 
GCCAAGTTAA AAGCTAAGCA GGCTTTAGAT TCAATACCAA AAAATAAGTT AACCCTACTT 
ATTAACTATG CAATGCGAAA CGTGTACTTA GCCCGGAATT ATTTTGCTGG AACGGAGGAC 
GAAGATGAAT TTGTAGATGA TGAAGTTATT GAAAAATTAC TTAACGCAAT GGATGCTTGC 
TTGCTTATAT GTAACATTTA TTCTACAGTA AGCGACCTTC AGTTCTTACA AGAAGATAAC 
GTATCACACA TTATAAAATT TACGCAATTT CAACTGCGTG AAACAATTTT TCCTCTTCAT 
GACCCGGTTT ACACGGCAAA AAGTATAAAA AGGACAACTC ATCGTAAAAA AATAAAATCA 
CACCAAGCTC AAAATCGAAG TATGCAGCTA TTCTACTTGA AAACTGTAGA GCTATTAAAA 
GTTTTTGTTA CACTTTTTGA CAAATGTGTA TTTGTTGATA CTATAGTTTT ACCATTGTCG 
ACACTGGCTA TTGAACCATT TTTTGTTGAC AACATAGAGA CGTTGCAATT TGTATGTTTG 
GAACTCGTTA CCACAATTTT TCGCAAGGAA AGATATGATA AAATAAGAAA CAGTATCTTA 
GGCGACATTT TGACATCTAT TGATCGGTTA CCTTCATCAA AAAAAAATCT CCGCCCATAT 
AAACTCACAA ACAATGGCGG AAATATTCAA ATGGTTACAG CACTTGTATT GCAATTGATT 
CAATGCGCCA CGATTTTACC AGATTCCCTT TGTGATAATG GCAAATTTAG TAACAAACCC 
CAAGAGGGAA ATACTTTTGA TGAAGAAGGA AAAAAATTGC TTCAGCCTAG TCAGGATCTA 
CTCGTTTTAC AAAAATATGA TGTAGCTGTC AGCATTGGTG GAAATTTTTT AACAACTTTC 
CTTAACAAAT GCAAATCTCG GTCTAATGAA ACTGATTTTC GACCTCTGTT TGAAAATTTT 
ATTCATGATT TACTGGCCAC CGTGAATAAG CCTGAATGGC CTGCATCTGA ATTGTTGCTT 
TCGCTGTTGG GAACTATGCT GGTTCGCTAC GTTTCTGATA AGGGTATAGA ACAAAGCATT 
CGCTTGGTAT CATTGGATTA CCTGGGCATA GTAGCTGCTC GATTGCGAAA AGACACAGTG 
GAATCTCGGT GTAGAGTTAA CATTATTGAT TCAATGATTC AATCCATCAA ATTAGAGCAA 
GAAAAGGAGG GAGATGTAAC ATCAAACAAT GATCAATTTG ATCTAGAACC CGAGGAGCAG 
CGAACGGATT TTTTGCAAAA GATTCTTCTC GACTTTCTAG CTGTAAATGC TCAAGAAGAA 
AACTTAATCT GGGACTATGC TAGACATTTT TATTTGGCAC AATGGTATCG GGATGTCATA 
TATCAAAGAC GTCGGATAAA TGATGGCAAA AAAGGTTTGG CTTTCAGGAA GTCCAAAATT 
CGTAATAATC GGAGGACTAA TGGTGATTAT TTAGATACGT CGGATTCTGG ATCGTGCGAT 
GAAAGTGATA CCGATACAAA CAAAAAGAGA ATTCACTGTG TGGACAGCAA TGATTTCGAA 
CTAAATATTA ACATCTATAA GGCTTTAGAA GCAAGAAAGC AATATTTTAT CAACAAAATT 
AAGCCATTTT CAGTTTTTGG CGAGCAAAAC CACTCGTCAA ATCAACACAT CAAAACATAC 
ATAGATTATA ATAACGCACA GTTAATAGCA CAATATCTGG CCACCAAAAG GCCCTTCAGT 
CAATCTTTTG ATGGATGCTT AAAGAAAATT ATATTAGTTG TTAATGAGCC ATCTATAGCA 
GTAAGGACAC GTGCAATGAA ATGTCTTGCC AACATAGTGG AAGTTGATCC ATTGGTTCTA 
AAACGAAAAG ATATGCAAAT GGGAGTTAAT CAGAAATTCC TAGACACAGC TATTTCGGTA 
CGAGAAGCTG CAGTAGATTT GGTTGGAAAA TTTGTTCTAA GTAATCAAGA TCTAATCGAT 
CAGTATTACG ATATGCTGTC TACTCGTATT TTGGATACTG GTGTCTCCGT ACGGAAAAGG 
GTCATTAAAA TTTTGAGAGA TATTTGTTTA GAATATCCAG ACTTTTCAAA AATTCCCGAA 
ATTTGCGTTA AAATGATTCG ACGTGTTCAT GACGAAGAAG GCATACAAAA ACTTGTCACT 
GAAGTTTTTA TGAAGATGTG GTTTACACCT TGTACTAAAA ACGACAAAAT TGGAATACAA 
AGGAAAATAA ATCATATAAT TGACGTGGTA AATACTGCAC ATGATACTGG AACTACTTGG 
TTAGAGGGCC TTCTAATGAG TATATTTAAG CCAAGAGATA ATATGCTAAG GTCTGAAGGA 
TGCGTTCAAG AATTTATTAA GAAGAACTCA GAGCCACCAA TGGATATTGT GATAGCTTGT 
CAACAATTAG CCGACGGACT AGTTGACAGA CTAATTGAAT TGGAAGATAC AGATAACTCG 
CGAATGCTGG GTTGTATTAC CACACTTCAT TTACTGGCTA AAGTGCGGCC CCAACTGCTT 
GTTAAGCATG CTATAACAAT TGAACCATAT CTCAACATAA AATGTCATTC TGCCACAGCC 
GCCAAGTTCA TATGTGCAGT TGCAGATATT CTCGAAAAAG TAGTTCCTCT GGTTAATAAT 
GCCAGTGAAT CCTTTTTAGC ATCGTTGGAA GAGCATTTAA TGTTATTGGT TGTTTCACGA 
AATCAAGCCG AAGTTACTAG CTGTGTTTCT TGCTTGGGAG CACTTGTGAA TAAGATTACA 
CATAACTTTA AATTGATCCG CGACTGTTTT CAAAAATTTT ATCGGGTTCT TGATGTGTCT 
CGAAGTCAAG TAATACAAGG AAATAACAGC GTAGATAATA TATACACTCC CAGCTTTCGG 
CGAAGTTTGT TTACGATCGG AATACTAATG CGATATTTCG ACTTTAAGTC TCCTATTGCG 
TTAGGTGAAA CAAATGATGG ACTTCCTGTT TCTATATGTG AGGATGTATT CCATTGCTTG 
ATGTTCTTCT GCCGTTGTAC TAATCAAGAA ATTCGAAAGC AAGCACTTAT TTCGCTTGGA 
TCATTTTGTG TACTAAACGA TGGCTACCTT ACAAGATCCG AACTTAAAAA TTTGTATTGC 
GAAATTTTAA GTTCAATAGC CAACGACGCC GGCTTTAAAA TAATCTGCAT GCGAAATATT 
TGGATTTATC TAACGGAATC TGAAATGTTT ATGCACAATA AGGAAAAAGA ATGGGAAAAA 
CAATCAAAAC ACGAAGATCT TAAGGAAATG AACGACGTTT CGTCTGGCAT GGCAAGTCGA 
ATAATTCAAC TATATTTAGA AGAAATTCTT GAATGCTTTC TTAATCGGGA TGACACAGTT 
CGGTTATGGG CTGTCAAAGT AATACAGATT GTCCTCCGTC AAGGTTTGGT TCACCCTGTT 
AGAATGGTTC CGTATTTAAT TTGCCTGAGT ACTGATCATA GGATAGAGTC TGCTCATCGA 
GCTGATGCCC TGTTGAAAGA TATTGATAAA ACATACTCTG GATTTGTTAA TATGAAAGTG 
CAATTCGGGC TACAGCTTTG CTTTAAGCTA CAAAAAATTT TACAAATAAA CAACAGAGGG 
AAACTGGAAA TAATTAGAGG TTATGCGAGT CGTGGACCAG ATAATACAAC TACCGCTTTA 
AACGATTTTC TGTATACACT TCTTCGCACT ACTAAACCGC AAAGGCGTGC TTTGGTGCAA 
ACAGTTACAA AACAGTTTGA CGATCAGAAA ACATCATTAC AACAAATGTT ATATATTGCA 
GATAACCTGG CTTACTTTCC ATATGTAGTT CAAGATGAAC CTTTATATCT AATCCATCAA 
ATAGATCTTC TAATTTCTAT GGCTGGAACA CATTTACTTG CTACATTCAA GGAACATATA 
AAACCAAGCG ACAAGGAAGG AGATGTTTTA GAAGATGATG ATGACGTAGA AGATCCAGAG 
GTGTTGTTTA ATAGATTGCC CGAAGATTTG ACGGAAATTA TTAAATGTAT AACATCTGCT 
CAAGCATGTA TGCTTCTTTT AATACTGAAA GATCATCTGA AGGAAATGTA TGCGATTACT 
GACAGTAAAA TTTCAAGATA TTCACCTTCC GAACAAAAAT TATATGAAAA AGCCGTAACA 
CGTAAAAGTG TTAATGATTT TAACCCAAAA ACGACTATTG ATGTTATAAA GAAACAAATG 
TCGCAAGAGA AATTGAGTAC GGACATTAAT TTTACACTGA CTAAAGAGGA GAAATTGGAC 
CTTGTTGTTA AATACCTTGA TTTTAAGCAA CTGATGCTTA AATTGGATCC GGATGATGGA 
GACTCCGATG CTGATGAATC CCGCGACAAA ACAATGTTAA ATATCTCTGC TTCTTCGGAT 
GGAGTGGCAT TCTCTAGTTC AGCAAAAAAT TCTCACTCCG CTTGTGATGG ATACTCACTA 
ACAGTCACGG ATGTGGTTGA TGTCCCAATG TCTCATATTG CGAAGGCATC TATGTTGACT 
TCAAAACCTA GTGGTCGCAA AACAAATCCT GTGCGAACTA AAAAAAAAAG AAGAAAAATT 
GATTCTACGG ATGATGAAAC CAGTGATGCG GAATATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163053.1
CDS	292..6453
Translation	MGERDNPTVPVTTLAGLTSTSDLLSELPVADSLQSAASLNKSLLFHALVANESSNLLSMRNENLVRQLVTAIERTNSDNIELIHCPVQDTATNCSTYPELLQGIYHFRPAVFNTSIKIHSPDQHTANARLEAKKMQIDSYSIPECYASPTNLSISNEHQLQDAQACFNQLTSMTSKEILEEFSQINFKENATEKDKIDVIENSDLNVTKILSQNTLKKKSNTPERSTVQSIQEQFFIQQQEANYNLKHDLNQVSTNVGQIQNISDTFPQEPSNFNLNSVNIPNMLYVQSPPFTESEPTQAHHSSIEYLKKKKSQILDVSILNRQQVLENTSLHIINKSSTYQTNQNVSTTTSTSTSSSGKSQVRSPELARSMGLLQETCQQQHENLNIIPTSAEECVLESRASSTNNTVKIPIDTFSTIKADEKRSAKRKLAISIGDIPPEQIFSKPKMRRVERITPLSNTKCVKEEVTRSQTYQQFIRNMDHIIEILDDSESPNFDSEDVDETIECISSKLLNSMSTDVAKLKAKQALDSIPKNKLTLLINYAMRNVYLARNYFAGTEDEDEFVDDEVIEKLLNAMDACLLICNIYSTVSDLQFLQEDNVSHIIKFTQFQLRETIFPLHDPVYTAKSIKRTTHRKKIKSHQAQNRSMQLFYLKTVELLKVFVTLFDKCVFVDTIVLPLSTLAIEPFFVDNIETLQFVCLELVTTIFRKERYDKIRNSILGDILTSIDRLPSSKKNLRPYKLTNNGGNIQMVTALVLQLIQCATILPDSLCDNGKFSNKPQEGNTFDEEGKKLLQPSQDLLVLQKYDVAVSIGGNFLTTFLNKCKSRSNETDFRPLFENFIHDLLATVNKPEWPASELLLSLLGTMLVRYVSDKGIEQSIRLVSLDYLGIVAARLRKDTVESRCRVNIIDSMIQSIKLEQEKEGDVTSNNDQFDLEPEEQRTDFLQKILLDFLAVNAQEENLIWDYARHFYLAQWYRDVIYQRRRINDGKKGLAFRKSKIRNNRRTNGDYLDTSDSGSCDESDTDTNKKRIHCVDSNDFELNINIYKALEARKQYFINKIKPFSVFGEQNHSSNQHIKTYIDYNNAQLIAQYLATKRPFSQSFDGCLKKIILVVNEPSIAVRTRAMKCLANIVEVDPLVLKRKDMQMGVNQKFLDTAISVREAAVDLVGKFVLSNQDLIDQYYDMLSTRILDTGVSVRKRVIKILRDICLEYPDFSKIPEICVKMIRRVHDEEGIQKLVTEVFMKMWFTPCTKNDKIGIQRKINHIIDVVNTAHDTGTTWLEGLLMSIFKPRDNMLRSEGCVQEFIKKNSEPPMDIVIACQQLADGLVDRLIELEDTDNSRMLGCITTLHLLAKVRPQLLVKHAITIEPYLNIKCHSATAAKFICAVADILEKVVPLVNNASESFLASLEEHLMLLVVSRNQAEVTSCVSCLGALVNKITHNFKLIRDCFQKFYRVLDVSRSQVIQGNNSVDNIYTPSFRRSLFTIGILMRYFDFKSPIALGETNDGLPVSICEDVFHCLMFFCRCTNQEIRKQALISLGSFCVLNDGYLTRSELKNLYCEILSSIANDAGFKIICMRNIWIYLTESEMFMHNKEKEWEKQSKHEDLKEMNDVSSGMASRIIQLYLEEILECFLNRDDTVRLWAVKVIQIVLRQGLVHPVRMVPYLICLSTDHRIESAHRADALLKDIDKTYSGFVNMKVQFGLQLCFKLQKILQINNRGKLEIIRGYASRGPDNTTTALNDFLYTLLRTTKPQRRALVQTVTKQFDDQKTSLQQMLYIADNLAYFPYVVQDEPLYLIHQIDLLISMAGTHLLATFKEHIKPSDKEGDVLEDDDDVEDPEVLFNRLPEDLTEIIKCITSAQACMLLLILKDHLKEMYAITDSKISRYSPSEQKLYEKAVTRKSVNDFNPKTTIDVIKKQMSQEKLSTDINFTLTKEEKLDLVVKYLDFKQLMLKLDPDDGDSDADESRDKTMLNISASSDGVAFSSSAKNSHSACDGYSLTVTDVVDVPMSHIAKASMLTSKPSGRKTNPVRTKKKRRKIDSTDDETSDAEYA

Target ORF information:

RefSeq Version	NM_001169582.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nipped-B, transcript variant H (Nipped-B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169582.2

ORF Insert Sequence:

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGTCACCAGA ATTGGCTAGA AGCATGGGGT TACTACAAGA AACGTGCCAG 
CAGCAACATG AAAACTTGAA TATTATACCC ACGAGTGCGG AAGAATGTGT ATTAGAGAGT 
CGTGCTTCCA GTACAAATAA TACAGTCAAA ATCCCTATTG ATACATTTTC TACTATAAAA 
GCCGATGAAA AACGATCCGC GAAAAGAAAA CTTGCCATAT CAATTGGTGA CATCCCACCG 
GAACAAATAT TTTCAAAGCC TAAAATGCGT CGGGTCGAAA GAATTACACC TCTTTCGAAC 
ACAAAGTGTG TGAAGGAGGA AGTCACCCGT TCGCAAACAT ATCAACAATT TATTCGAAAC 
ATGGATCACA TAATTGAAAT ATTAGACGAT AGTGAATCTC CTAACTTTGA CTCAGAGGAT 
GTAGACGAAA CCATTGAATG CATTTCCTCA AAACTTTTAA ATTCAATGAG TACTGATGTT 
GCCAAGTTAA AAGCTAAGCA GGCTTTAGAT TCAATACCAA AAAATAAGTT AACCCTACTT 
ATTAACTATG CAATGCGAAA CGTGTACTTA GCCCGGAATT ATTTTGCTGG AACGGAGGAC 
GAAGATGAAT TTGTAGATGA TGAAGTTATT GAAAAATTAC TTAACGCAAT GGATGCTTGC 
TTGCTTATAT GTAACATTTA TTCTACAGTA AGCGACCTTC AGTTCTTACA AGAAGATAAC 
GTATCACACA TTATAAAATT TACGCAATTT CAACTGCGTG AAACAATTTT TCCTCTTCAT 
GACCCGGTTT ACACGGCAAA AAGTATAAAA AGGACAACTC ATCGTAAAAA AATAAAATCA 
CACCAAGCTC AAAATCGAAG TATGCAGCTA TTCTACTTGA AAACTGTAGA GCTATTAAAA 
GTTTTTGTTA CACTTTTTGA CAAATGTGTA TTTGTTGATA CTATAGTTTT ACCATTGTCG 
ACACTGGCTA TTGAACCATT TTTTGTTGAC AACATAGAGA CGTTGCAATT TGTATGTTTG 
GAACTCGTTA CCACAATTTT TCGCAAGGAA AGATATGATA AAATAAGAAA CAGTATCTTA 
GGCGACATTT TGACATCTAT TGATCGGTTA CCTTCATCAA AAAAAAATCT CCGCCCATAT 
AAACTCACAA ACAATGGCGG AAATATTCAA ATGGTTACAG CACTTGTATT GCAATTGATT 
CAATGCGCCA CGATTTTACC AGATTCCCTT TGTGATAATG GCAAATTTAG TAACAAACCC 
CAAGAGGGAA ATACTTTTGA TGAAGAAGGA AAAAAATTGC TTCAGCCTAG TCAGGATCTA 
CTCGTTTTAC AAAAATATGA TGTAGCTGTC AGCATTGGTG GAAATTTTTT AACAACTTTC 
CTTAACAAAT GCAAATCTCG GTCTAATGAA ACTGATTTTC GACCTCTGTT TGAAAATTTT 
ATTCATGATT TACTGGCCAC CGTGAATAAG CCTGAATGGC CTGCATCTGA ATTGTTGCTT 
TCGCTGTTGG GAACTATGCT GGTTCGCTAC GTTTCTGATA AGGGTATAGA ACAAAGCATT 
CGCTTGGTAT CATTGGATTA CCTGGGCATA GTAGCTGCTC GATTGCGAAA AGACACAGTG 
GAATCTCGGT GTAGAGTTAA CATTATTGAT TCAATGATTC AATCCATCAA ATTAGAGCAA 
GAAAAGGAGG GAGATGTAAC ATCAAACAAT GATCAATTTG ATCTAGAACC CGAGGAGCAG 
CGAACGGATT TTTTGCAAAA GATTCTTCTC GACTTTCTAG CTGTAAATGC TCAAGAAGAA 
AACTTAATCT GGGACTATGC TAGACATTTT TATTTGGCAC AATGGTATCG GGATGTCATA 
TATCAAAGAC GTCGGATAAA TGATGGCAAA AAAGGTTTGG CTTTCAGGAA GTCCAAAATT 
CGTAATAATC GGAGGACTAA TGGTGATTAT TTAGATACGT CGGATTCTGG ATCGTGCGAT 
GAAAGTGATA CCGATACAAA CAAAAAGAGA ATTCACTGTG TGGACAGCAA TGATTTCGAA 
CTAAATATTA ACATCTATAA GGCTTTAGAA GCAAGAAAGC AATATTTTAT CAACAAAATT 
AAGCCATTTT CAGTTTTTGG CGAGCAAAAC CACTCGTCAA ATCAACACAT CAAAACATAC 
ATAGATTATA ATAACGCACA GTTAATAGCA CAATATCTGG CCACCAAAAG GCCCTTCAGT 
CAATCTTTTG ATGGATGCTT AAAGAAAATT ATATTAGTTG TTAATGAGCC ATCTATAGCA 
GTAAGGACAC GTGCAATGAA ATGTCTTGCC AACATAGTGG AAGTTGATCC ATTGGTTCTA 
AAACGAAAAG ATATGCAAAT GGGAGTTAAT CAGAAATTCC TAGACACAGC TATTTCGGTA 
CGAGAAGCTG CAGTAGATTT GGTTGGAAAA TTTGTTCTAA GTAATCAAGA TCTAATCGAT 
CAGTATTACG ATATGCTGTC TACTCGTATT TTGGATACTG GTGTCTCCGT ACGGAAAAGG 
GTCATTAAAA TTTTGAGAGA TATTTGTTTA GAATATCCAG ACTTTTCAAA AATTCCCGAA 
ATTTGCGTTA AAATGATTCG ACGTGTTCAT GACGAAGAAG GCATACAAAA ACTTGTCACT 
GAAGTTTTTA TGAAGATGTG GTTTACACCT TGTACTAAAA ACGACAAAAT TGGAATACAA 
AGGAAAATAA ATCATATAAT TGACGTGGTA AATACTGCAC ATGATACTGG AACTACTTGG 
TTAGAGGGCC TTCTAATGAG TATATTTAAG CCAAGAGATA ATATGCTAAG GTCTGAAGGA 
TGCGTTCAAG AATTTATTAA GAAGAACTCA GAGCCACCAA TGGATATTGT GATAGCTTGT 
CAACAATTAG CCGACGGACT AGTTGACAGA CTAATTGAAT TGGAAGATAC AGATAACTCG 
CGAATGCTGG GTTGTATTAC CACACTTCAT TTACTGGCTA AAGTGCGGCC CCAACTGCTT 
GTTAAGCATG CTATAACAAT TGAACCATAT CTCAACATAA AATGTCATTC TGCCACAGCC 
GCCAAGTTCA TATGTGCAGT TGCAGATATT CTCGAAAAAG TAGTTCCTCT GGTTAATAAT 
GCCAGTGAAT CCTTTTTAGC ATCGTTGGAA GAGCATTTAA TGTTATTGGT TGTTTCACGA 
AATCAAGCCG AAGTTACTAG CTGTGTTTCT TGCTTGGGAG CACTTGTGAA TAAGATTACA 
CATAACTTTA AATTGATCCG CGACTGTTTT CAAAAATTTT ATCGGGTTCT TGATGTGTCT 
CGAAGTCAAG TAATACAAGG AAATAACAGC GTAGATAATA TATACACTCC CAGCTTTCGG 
CGAAGTTTGT TTACGATCGG AATACTAATG CGATATTTCG ACTTTAAGTC TCCTATTGCG 
TTAGGTGAAA CAAATGATGG ACTTCCTGTT TCTATATGTG AGGATGTATT CCATTGCTTG 
ATGTTCTTCT GCCGTTGTAC TAATCAAGAA ATTCGAAAGC AAGCACTTAT TTCGCTTGGA 
TCATTTTGTG TACTAAACGA TGGCTACCTT ACAAGATCCG AACTTAAAAA TTTGTATTGC 
GAAATTTTAA GTTCAATAGC CAACGACGCC GGCTTTAAAA TAATCTGCAT GCGAAATATT 
TGGATTTATC TAACGGAATC TGAAATGTTT ATGCACAATA AGGAAAAAGA ATGGGAAAAA 
CAATCAAAAC ACGAAGATCT TAAGGAAATG AACGACGTTT CGTCTGGCAT GGCAAGTCGA 
ATAATTCAAC TATATTTAGA AGAAATTCTT GAATGCTTTC TTAATCGGGA TGACACAGTT 
CGGTTATGGG CTGTCAAAGT AATACAGATT GTCCTCCGTC AAGGTTTGGT TCACCCTGTT 
AGAATGGTTC CGTATTTAAT TTGCCTGAGT ACTGATCATA GGATAGAGTC TGCTCATCGA 
GCTGATGCCC TGTTGAAAGA TATTGATAAA ACATACTCTG GATTTGTTAA TATGAAAGTG 
CAATTCGGGC TACAGCTTTG CTTTAAGCTA CAAAAAATTT TACAAATAAA CAACAGAGGG 
AAACTGGAAA TAATTAGAGG TTATGCGAGT CGTGGACCAG ATAATACAAC TACCGCTTTA 
AACGATTTTC TGTATACACT TCTTCGCACT ACTAAACCGC AAAGGCGTGC TTTGGTGCAA 
ACAGTTACAA AACAGTTTGA CGATCAGAAA ACATCATTAC AACAAATGTT ATATATTGCA 
GATAACCTGG CTTACTTTCC ATATGTAGTT CAAGATGAAC CTTTATATCT AATCCATCAA 
ATAGATCTTC TAATTTCTAT GGCTGGAACA CATTTACTTG CTACATTCAA GGAACATATA 
AAACCAAGCG ACAAGGAAGG AGATGTTTTA GAAGATGATG ATGACGTAGA AGATCCAGAG 
GTGTTGTTTA ATAGATTGCC CGAAGATTTG ACGGAAATTA TTAAATGTAT AACATCTGCT 
CAAGCATGTA TGCTTCTTTT AATACTGAAA GATCATCTGA AGGAAATGTA TGCGATTACT 
GACAGTAAAA TTTCAAGATA TTCACCTTCC GAACAAAAAT TATATGAAAA AGCCGTAACA 
CGTAAAAGTG TTAATGATTT TAACCCAAAA ACGACTATTG ATGTTATAAA GAAACAAATG 
TCGCAAGAGA AATTGAGTAC GGACATTAAT TTTACACTGA CTAAAGAGGA GAAATTGGAC 
CTTGTTGTTA AATACCTTGA TTTTAAGCAA CTGATGCTTA AATTGGATCC GGATGATGGA 
GACTCCGATG CTGATGAATC CCGCGACAAA ACAATGTTAA ATATCTCTGC TTCTTCGGAT 
GGAGTGGCAT TCTCTAGTTC AGCAAAAAAT TCTCACTCCG CTTGTGATGG ATACTCACTA 
ACAGTCACGG ATGTGGTTGA TGTCCCAATG TCTCATATTG CGAAGGCATC TATGTTGACT 
TCAAAACCTA GTGGTCGCAA AACAAATCCT GTGCGAACTA AAAAAAAAAG AAGAAAAATT 
GATTCTACGG ATGATGAAAC CAGTGATGCG GAATATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipped-B ( NM_001169581.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163052.1 Drosophila melanogaster Nipped-B, transcript variant G (Nipped-B), mRNA.

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CloneID	OFa13073
Clone ID Related Accession (Same CDS sequence)	NM_001169583.2 , NM_001169582.2 , NM_001169581.2
Accession Version	NM_001169581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nipped-B, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169581.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGTCACCAGA ATTGGCTAGA AGCATGGGGT TACTACAAGA AACGTGCCAG 
CAGCAACATG AAAACTTGAA TATTATACCC ACGAGTGCGG AAGAATGTGT ATTAGAGAGT 
CGTGCTTCCA GTACAAATAA TACAGTCAAA ATCCCTATTG ATACATTTTC TACTATAAAA 
GCCGATGAAA AACGATCCGC GAAAAGAAAA CTTGCCATAT CAATTGGTGA CATCCCACCG 
GAACAAATAT TTTCAAAGCC TAAAATGCGT CGGGTCGAAA GAATTACACC TCTTTCGAAC 
ACAAAGTGTG TGAAGGAGGA AGTCACCCGT TCGCAAACAT ATCAACAATT TATTCGAAAC 
ATGGATCACA TAATTGAAAT ATTAGACGAT AGTGAATCTC CTAACTTTGA CTCAGAGGAT 
GTAGACGAAA CCATTGAATG CATTTCCTCA AAACTTTTAA ATTCAATGAG TACTGATGTT 
GCCAAGTTAA AAGCTAAGCA GGCTTTAGAT TCAATACCAA AAAATAAGTT AACCCTACTT 
ATTAACTATG CAATGCGAAA CGTGTACTTA GCCCGGAATT ATTTTGCTGG AACGGAGGAC 
GAAGATGAAT TTGTAGATGA TGAAGTTATT GAAAAATTAC TTAACGCAAT GGATGCTTGC 
TTGCTTATAT GTAACATTTA TTCTACAGTA AGCGACCTTC AGTTCTTACA AGAAGATAAC 
GTATCACACA TTATAAAATT TACGCAATTT CAACTGCGTG AAACAATTTT TCCTCTTCAT 
GACCCGGTTT ACACGGCAAA AAGTATAAAA AGGACAACTC ATCGTAAAAA AATAAAATCA 
CACCAAGCTC AAAATCGAAG TATGCAGCTA TTCTACTTGA AAACTGTAGA GCTATTAAAA 
GTTTTTGTTA CACTTTTTGA CAAATGTGTA TTTGTTGATA CTATAGTTTT ACCATTGTCG 
ACACTGGCTA TTGAACCATT TTTTGTTGAC AACATAGAGA CGTTGCAATT TGTATGTTTG 
GAACTCGTTA CCACAATTTT TCGCAAGGAA AGATATGATA AAATAAGAAA CAGTATCTTA 
GGCGACATTT TGACATCTAT TGATCGGTTA CCTTCATCAA AAAAAAATCT CCGCCCATAT 
AAACTCACAA ACAATGGCGG AAATATTCAA ATGGTTACAG CACTTGTATT GCAATTGATT 
CAATGCGCCA CGATTTTACC AGATTCCCTT TGTGATAATG GCAAATTTAG TAACAAACCC 
CAAGAGGGAA ATACTTTTGA TGAAGAAGGA AAAAAATTGC TTCAGCCTAG TCAGGATCTA 
CTCGTTTTAC AAAAATATGA TGTAGCTGTC AGCATTGGTG GAAATTTTTT AACAACTTTC 
CTTAACAAAT GCAAATCTCG GTCTAATGAA ACTGATTTTC GACCTCTGTT TGAAAATTTT 
ATTCATGATT TACTGGCCAC CGTGAATAAG CCTGAATGGC CTGCATCTGA ATTGTTGCTT 
TCGCTGTTGG GAACTATGCT GGTTCGCTAC GTTTCTGATA AGGGTATAGA ACAAAGCATT 
CGCTTGGTAT CATTGGATTA CCTGGGCATA GTAGCTGCTC GATTGCGAAA AGACACAGTG 
GAATCTCGGT GTAGAGTTAA CATTATTGAT TCAATGATTC AATCCATCAA ATTAGAGCAA 
GAAAAGGAGG GAGATGTAAC ATCAAACAAT GATCAATTTG ATCTAGAACC CGAGGAGCAG 
CGAACGGATT TTTTGCAAAA GATTCTTCTC GACTTTCTAG CTGTAAATGC TCAAGAAGAA 
AACTTAATCT GGGACTATGC TAGACATTTT TATTTGGCAC AATGGTATCG GGATGTCATA 
TATCAAAGAC GTCGGATAAA TGATGGCAAA AAAGGTTTGG CTTTCAGGAA GTCCAAAATT 
CGTAATAATC GGAGGACTAA TGGTGATTAT TTAGATACGT CGGATTCTGG ATCGTGCGAT 
GAAAGTGATA CCGATACAAA CAAAAAGAGA ATTCACTGTG TGGACAGCAA TGATTTCGAA 
CTAAATATTA ACATCTATAA GGCTTTAGAA GCAAGAAAGC AATATTTTAT CAACAAAATT 
AAGCCATTTT CAGTTTTTGG CGAGCAAAAC CACTCGTCAA ATCAACACAT CAAAACATAC 
ATAGATTATA ATAACGCACA GTTAATAGCA CAATATCTGG CCACCAAAAG GCCCTTCAGT 
CAATCTTTTG ATGGATGCTT AAAGAAAATT ATATTAGTTG TTAATGAGCC ATCTATAGCA 
GTAAGGACAC GTGCAATGAA ATGTCTTGCC AACATAGTGG AAGTTGATCC ATTGGTTCTA 
AAACGAAAAG ATATGCAAAT GGGAGTTAAT CAGAAATTCC TAGACACAGC TATTTCGGTA 
CGAGAAGCTG CAGTAGATTT GGTTGGAAAA TTTGTTCTAA GTAATCAAGA TCTAATCGAT 
CAGTATTACG ATATGCTGTC TACTCGTATT TTGGATACTG GTGTCTCCGT ACGGAAAAGG 
GTCATTAAAA TTTTGAGAGA TATTTGTTTA GAATATCCAG ACTTTTCAAA AATTCCCGAA 
ATTTGCGTTA AAATGATTCG ACGTGTTCAT GACGAAGAAG GCATACAAAA ACTTGTCACT 
GAAGTTTTTA TGAAGATGTG GTTTACACCT TGTACTAAAA ACGACAAAAT TGGAATACAA 
AGGAAAATAA ATCATATAAT TGACGTGGTA AATACTGCAC ATGATACTGG AACTACTTGG 
TTAGAGGGCC TTCTAATGAG TATATTTAAG CCAAGAGATA ATATGCTAAG GTCTGAAGGA 
TGCGTTCAAG AATTTATTAA GAAGAACTCA GAGCCACCAA TGGATATTGT GATAGCTTGT 
CAACAATTAG CCGACGGACT AGTTGACAGA CTAATTGAAT TGGAAGATAC AGATAACTCG 
CGAATGCTGG GTTGTATTAC CACACTTCAT TTACTGGCTA AAGTGCGGCC CCAACTGCTT 
GTTAAGCATG CTATAACAAT TGAACCATAT CTCAACATAA AATGTCATTC TGCCACAGCC 
GCCAAGTTCA TATGTGCAGT TGCAGATATT CTCGAAAAAG TAGTTCCTCT GGTTAATAAT 
GCCAGTGAAT CCTTTTTAGC ATCGTTGGAA GAGCATTTAA TGTTATTGGT TGTTTCACGA 
AATCAAGCCG AAGTTACTAG CTGTGTTTCT TGCTTGGGAG CACTTGTGAA TAAGATTACA 
CATAACTTTA AATTGATCCG CGACTGTTTT CAAAAATTTT ATCGGGTTCT TGATGTGTCT 
CGAAGTCAAG TAATACAAGG AAATAACAGC GTAGATAATA TATACACTCC CAGCTTTCGG 
CGAAGTTTGT TTACGATCGG AATACTAATG CGATATTTCG ACTTTAAGTC TCCTATTGCG 
TTAGGTGAAA CAAATGATGG ACTTCCTGTT TCTATATGTG AGGATGTATT CCATTGCTTG 
ATGTTCTTCT GCCGTTGTAC TAATCAAGAA ATTCGAAAGC AAGCACTTAT TTCGCTTGGA 
TCATTTTGTG TACTAAACGA TGGCTACCTT ACAAGATCCG AACTTAAAAA TTTGTATTGC 
GAAATTTTAA GTTCAATAGC CAACGACGCC GGCTTTAAAA TAATCTGCAT GCGAAATATT 
TGGATTTATC TAACGGAATC TGAAATGTTT ATGCACAATA AGGAAAAAGA ATGGGAAAAA 
CAATCAAAAC ACGAAGATCT TAAGGAAATG AACGACGTTT CGTCTGGCAT GGCAAGTCGA 
ATAATTCAAC TATATTTAGA AGAAATTCTT GAATGCTTTC TTAATCGGGA TGACACAGTT 
CGGTTATGGG CTGTCAAAGT AATACAGATT GTCCTCCGTC AAGGTTTGGT TCACCCTGTT 
AGAATGGTTC CGTATTTAAT TTGCCTGAGT ACTGATCATA GGATAGAGTC TGCTCATCGA 
GCTGATGCCC TGTTGAAAGA TATTGATAAA ACATACTCTG GATTTGTTAA TATGAAAGTG 
CAATTCGGGC TACAGCTTTG CTTTAAGCTA CAAAAAATTT TACAAATAAA CAACAGAGGG 
AAACTGGAAA TAATTAGAGG TTATGCGAGT CGTGGACCAG ATAATACAAC TACCGCTTTA 
AACGATTTTC TGTATACACT TCTTCGCACT ACTAAACCGC AAAGGCGTGC TTTGGTGCAA 
ACAGTTACAA AACAGTTTGA CGATCAGAAA ACATCATTAC AACAAATGTT ATATATTGCA 
GATAACCTGG CTTACTTTCC ATATGTAGTT CAAGATGAAC CTTTATATCT AATCCATCAA 
ATAGATCTTC TAATTTCTAT GGCTGGAACA CATTTACTTG CTACATTCAA GGAACATATA 
AAACCAAGCG ACAAGGAAGG AGATGTTTTA GAAGATGATG ATGACGTAGA AGATCCAGAG 
GTGTTGTTTA ATAGATTGCC CGAAGATTTG ACGGAAATTA TTAAATGTAT AACATCTGCT 
CAAGCATGTA TGCTTCTTTT AATACTGAAA GATCATCTGA AGGAAATGTA TGCGATTACT 
GACAGTAAAA TTTCAAGATA TTCACCTTCC GAACAAAAAT TATATGAAAA AGCCGTAACA 
CGTAAAAGTG TTAATGATTT TAACCCAAAA ACGACTATTG ATGTTATAAA GAAACAAATG 
TCGCAAGAGA AATTGAGTAC GGACATTAAT TTTACACTGA CTAAAGAGGA GAAATTGGAC 
CTTGTTGTTA AATACCTTGA TTTTAAGCAA CTGATGCTTA AATTGGATCC GGATGATGGA 
GACTCCGATG CTGATGAATC CCGCGACAAA ACAATGTTAA ATATCTCTGC TTCTTCGGAT 
GGAGTGGCAT TCTCTAGTTC AGCAAAAAAT TCTCACTCCG CTTGTGATGG ATACTCACTA 
ACAGTCACGG ATGTGGTTGA TGTCCCAATG TCTCATATTG CGAAGGCATC TATGTTGACT 
TCAAAACCTA GTGGTCGCAA AACAAATCCT GTGCGAACTA AAAAAAAAAG AAGAAAAATT 
GATTCTACGG ATGATGAAAC CAGTGATGCG GAATATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163052.1
CDS	257..6418
Translation	MGERDNPTVPVTTLAGLTSTSDLLSELPVADSLQSAASLNKSLLFHALVANESSNLLSMRNENLVRQLVTAIERTNSDNIELIHCPVQDTATNCSTYPELLQGIYHFRPAVFNTSIKIHSPDQHTANARLEAKKMQIDSYSIPECYASPTNLSISNEHQLQDAQACFNQLTSMTSKEILEEFSQINFKENATEKDKIDVIENSDLNVTKILSQNTLKKKSNTPERSTVQSIQEQFFIQQQEANYNLKHDLNQVSTNVGQIQNISDTFPQEPSNFNLNSVNIPNMLYVQSPPFTESEPTQAHHSSIEYLKKKKSQILDVSILNRQQVLENTSLHIINKSSTYQTNQNVSTTTSTSTSSSGKSQVRSPELARSMGLLQETCQQQHENLNIIPTSAEECVLESRASSTNNTVKIPIDTFSTIKADEKRSAKRKLAISIGDIPPEQIFSKPKMRRVERITPLSNTKCVKEEVTRSQTYQQFIRNMDHIIEILDDSESPNFDSEDVDETIECISSKLLNSMSTDVAKLKAKQALDSIPKNKLTLLINYAMRNVYLARNYFAGTEDEDEFVDDEVIEKLLNAMDACLLICNIYSTVSDLQFLQEDNVSHIIKFTQFQLRETIFPLHDPVYTAKSIKRTTHRKKIKSHQAQNRSMQLFYLKTVELLKVFVTLFDKCVFVDTIVLPLSTLAIEPFFVDNIETLQFVCLELVTTIFRKERYDKIRNSILGDILTSIDRLPSSKKNLRPYKLTNNGGNIQMVTALVLQLIQCATILPDSLCDNGKFSNKPQEGNTFDEEGKKLLQPSQDLLVLQKYDVAVSIGGNFLTTFLNKCKSRSNETDFRPLFENFIHDLLATVNKPEWPASELLLSLLGTMLVRYVSDKGIEQSIRLVSLDYLGIVAARLRKDTVESRCRVNIIDSMIQSIKLEQEKEGDVTSNNDQFDLEPEEQRTDFLQKILLDFLAVNAQEENLIWDYARHFYLAQWYRDVIYQRRRINDGKKGLAFRKSKIRNNRRTNGDYLDTSDSGSCDESDTDTNKKRIHCVDSNDFELNINIYKALEARKQYFINKIKPFSVFGEQNHSSNQHIKTYIDYNNAQLIAQYLATKRPFSQSFDGCLKKIILVVNEPSIAVRTRAMKCLANIVEVDPLVLKRKDMQMGVNQKFLDTAISVREAAVDLVGKFVLSNQDLIDQYYDMLSTRILDTGVSVRKRVIKILRDICLEYPDFSKIPEICVKMIRRVHDEEGIQKLVTEVFMKMWFTPCTKNDKIGIQRKINHIIDVVNTAHDTGTTWLEGLLMSIFKPRDNMLRSEGCVQEFIKKNSEPPMDIVIACQQLADGLVDRLIELEDTDNSRMLGCITTLHLLAKVRPQLLVKHAITIEPYLNIKCHSATAAKFICAVADILEKVVPLVNNASESFLASLEEHLMLLVVSRNQAEVTSCVSCLGALVNKITHNFKLIRDCFQKFYRVLDVSRSQVIQGNNSVDNIYTPSFRRSLFTIGILMRYFDFKSPIALGETNDGLPVSICEDVFHCLMFFCRCTNQEIRKQALISLGSFCVLNDGYLTRSELKNLYCEILSSIANDAGFKIICMRNIWIYLTESEMFMHNKEKEWEKQSKHEDLKEMNDVSSGMASRIIQLYLEEILECFLNRDDTVRLWAVKVIQIVLRQGLVHPVRMVPYLICLSTDHRIESAHRADALLKDIDKTYSGFVNMKVQFGLQLCFKLQKILQINNRGKLEIIRGYASRGPDNTTTALNDFLYTLLRTTKPQRRALVQTVTKQFDDQKTSLQQMLYIADNLAYFPYVVQDEPLYLIHQIDLLISMAGTHLLATFKEHIKPSDKEGDVLEDDDDVEDPEVLFNRLPEDLTEIIKCITSAQACMLLLILKDHLKEMYAITDSKISRYSPSEQKLYEKAVTRKSVNDFNPKTTIDVIKKQMSQEKLSTDINFTLTKEEKLDLVVKYLDFKQLMLKLDPDDGDSDADESRDKTMLNISASSDGVAFSSSAKNSHSACDGYSLTVTDVVDVPMSHIAKASMLTSKPSGRKTNPVRTKKKRRKIDSTDDETSDAEYA

Target ORF information:

RefSeq Version	NM_001169581.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nipped-B, transcript variant G (Nipped-B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169581.2

ORF Insert Sequence:

ATGGGGGAAC GTGACAACCC AACCGTACCT GTAACAACAT TAGCTGGTTT AACCAGCACT 
TCTGATTTGC TAAGCGAGCT ACCAGTGGCG GATTCGCTCC AGAGTGCTGC GTCCTTAAAT 
AAATCTTTAT TGTTCCATGC TTTGGTAGCT AACGAGTCTA GCAATTTACT TTCTATGCGA 
AACGAAAATC TAGTTCGACA ATTGGTAACT GCCATTGAGA GAACAAACTC CGATAATATA 
GAGTTAATAC ATTGCCCAGT TCAAGACACA GCAACTAACT GTAGTACTTA TCCTGAGCTT 
CTCCAGGGAA TATATCATTT TCGCCCAGCG GTTTTTAACA CTTCCATAAA AATACACTCT 
CCGGACCAAC ATACTGCTAA TGCTCGATTA GAGGCAAAGA AAATGCAAAT TGATTCCTAT 
TCAATACCGG AATGTTATGC ATCACCAACA AATTTGTCGA TTTCAAATGA ACATCAATTG 
CAAGACGCTC AAGCCTGCTT CAATCAGCTT ACTAGTATGA CCTCAAAAGA AATCTTAGAA 
GAATTTTCAC AAATAAATTT TAAGGAAAAT GCAACAGAAA AAGATAAAAT TGACGTTATT 
GAAAATTCAG ATTTAAATGT GACTAAGATA CTTTCACAAA ATACTCTAAA AAAAAAGTCT 
AACACACCAG AAAGAAGTAC GGTGCAATCA ATACAAGAAC AGTTTTTTAT TCAGCAACAA 
GAAGCCAATT ATAATTTAAA ACATGATTTA AACCAAGTAT CAACAAATGT TGGTCAAATT 
CAAAATATTT CTGATACTTT TCCCCAAGAG CCGTCAAATT TTAATTTAAA TTCTGTAAAT 
ATCCCAAACA TGTTATATGT ACAAAGTCCA CCTTTCACTG AATCAGAACC AACTCAAGCA 
CACCACTCTT CAATTGAATA TTTAAAAAAA AAAAAATCAC AAATTCTTGA CGTCAGCATT 
TTAAATCGAC AACAGGTGTT AGAAAATACC AGCTTACATA TTATTAATAA AAGTTCAACG 
TACCAAACGA ACCAAAATGT ATCAACAACT ACATCGACAT CTACTTCCTC AAGTGGCAAG 
TCCCAGGTCC GGTCACCAGA ATTGGCTAGA AGCATGGGGT TACTACAAGA AACGTGCCAG 
CAGCAACATG AAAACTTGAA TATTATACCC ACGAGTGCGG AAGAATGTGT ATTAGAGAGT 
CGTGCTTCCA GTACAAATAA TACAGTCAAA ATCCCTATTG ATACATTTTC TACTATAAAA 
GCCGATGAAA AACGATCCGC GAAAAGAAAA CTTGCCATAT CAATTGGTGA CATCCCACCG 
GAACAAATAT TTTCAAAGCC TAAAATGCGT CGGGTCGAAA GAATTACACC TCTTTCGAAC 
ACAAAGTGTG TGAAGGAGGA AGTCACCCGT TCGCAAACAT ATCAACAATT TATTCGAAAC 
ATGGATCACA TAATTGAAAT ATTAGACGAT AGTGAATCTC CTAACTTTGA CTCAGAGGAT 
GTAGACGAAA CCATTGAATG CATTTCCTCA AAACTTTTAA ATTCAATGAG TACTGATGTT 
GCCAAGTTAA AAGCTAAGCA GGCTTTAGAT TCAATACCAA AAAATAAGTT AACCCTACTT 
ATTAACTATG CAATGCGAAA CGTGTACTTA GCCCGGAATT ATTTTGCTGG AACGGAGGAC 
GAAGATGAAT TTGTAGATGA TGAAGTTATT GAAAAATTAC TTAACGCAAT GGATGCTTGC 
TTGCTTATAT GTAACATTTA TTCTACAGTA AGCGACCTTC AGTTCTTACA AGAAGATAAC 
GTATCACACA TTATAAAATT TACGCAATTT CAACTGCGTG AAACAATTTT TCCTCTTCAT 
GACCCGGTTT ACACGGCAAA AAGTATAAAA AGGACAACTC ATCGTAAAAA AATAAAATCA 
CACCAAGCTC AAAATCGAAG TATGCAGCTA TTCTACTTGA AAACTGTAGA GCTATTAAAA 
GTTTTTGTTA CACTTTTTGA CAAATGTGTA TTTGTTGATA CTATAGTTTT ACCATTGTCG 
ACACTGGCTA TTGAACCATT TTTTGTTGAC AACATAGAGA CGTTGCAATT TGTATGTTTG 
GAACTCGTTA CCACAATTTT TCGCAAGGAA AGATATGATA AAATAAGAAA CAGTATCTTA 
GGCGACATTT TGACATCTAT TGATCGGTTA CCTTCATCAA AAAAAAATCT CCGCCCATAT 
AAACTCACAA ACAATGGCGG AAATATTCAA ATGGTTACAG CACTTGTATT GCAATTGATT 
CAATGCGCCA CGATTTTACC AGATTCCCTT TGTGATAATG GCAAATTTAG TAACAAACCC 
CAAGAGGGAA ATACTTTTGA TGAAGAAGGA AAAAAATTGC TTCAGCCTAG TCAGGATCTA 
CTCGTTTTAC AAAAATATGA TGTAGCTGTC AGCATTGGTG GAAATTTTTT AACAACTTTC 
CTTAACAAAT GCAAATCTCG GTCTAATGAA ACTGATTTTC GACCTCTGTT TGAAAATTTT 
ATTCATGATT TACTGGCCAC CGTGAATAAG CCTGAATGGC CTGCATCTGA ATTGTTGCTT 
TCGCTGTTGG GAACTATGCT GGTTCGCTAC GTTTCTGATA AGGGTATAGA ACAAAGCATT 
CGCTTGGTAT CATTGGATTA CCTGGGCATA GTAGCTGCTC GATTGCGAAA AGACACAGTG 
GAATCTCGGT GTAGAGTTAA CATTATTGAT TCAATGATTC AATCCATCAA ATTAGAGCAA 
GAAAAGGAGG GAGATGTAAC ATCAAACAAT GATCAATTTG ATCTAGAACC CGAGGAGCAG 
CGAACGGATT TTTTGCAAAA GATTCTTCTC GACTTTCTAG CTGTAAATGC TCAAGAAGAA 
AACTTAATCT GGGACTATGC TAGACATTTT TATTTGGCAC AATGGTATCG GGATGTCATA 
TATCAAAGAC GTCGGATAAA TGATGGCAAA AAAGGTTTGG CTTTCAGGAA GTCCAAAATT 
CGTAATAATC GGAGGACTAA TGGTGATTAT TTAGATACGT CGGATTCTGG ATCGTGCGAT 
GAAAGTGATA CCGATACAAA CAAAAAGAGA ATTCACTGTG TGGACAGCAA TGATTTCGAA 
CTAAATATTA ACATCTATAA GGCTTTAGAA GCAAGAAAGC AATATTTTAT CAACAAAATT 
AAGCCATTTT CAGTTTTTGG CGAGCAAAAC CACTCGTCAA ATCAACACAT CAAAACATAC 
ATAGATTATA ATAACGCACA GTTAATAGCA CAATATCTGG CCACCAAAAG GCCCTTCAGT 
CAATCTTTTG ATGGATGCTT AAAGAAAATT ATATTAGTTG TTAATGAGCC ATCTATAGCA 
GTAAGGACAC GTGCAATGAA ATGTCTTGCC AACATAGTGG AAGTTGATCC ATTGGTTCTA 
AAACGAAAAG ATATGCAAAT GGGAGTTAAT CAGAAATTCC TAGACACAGC TATTTCGGTA 
CGAGAAGCTG CAGTAGATTT GGTTGGAAAA TTTGTTCTAA GTAATCAAGA TCTAATCGAT 
CAGTATTACG ATATGCTGTC TACTCGTATT TTGGATACTG GTGTCTCCGT ACGGAAAAGG 
GTCATTAAAA TTTTGAGAGA TATTTGTTTA GAATATCCAG ACTTTTCAAA AATTCCCGAA 
ATTTGCGTTA AAATGATTCG ACGTGTTCAT GACGAAGAAG GCATACAAAA ACTTGTCACT 
GAAGTTTTTA TGAAGATGTG GTTTACACCT TGTACTAAAA ACGACAAAAT TGGAATACAA 
AGGAAAATAA ATCATATAAT TGACGTGGTA AATACTGCAC ATGATACTGG AACTACTTGG 
TTAGAGGGCC TTCTAATGAG TATATTTAAG CCAAGAGATA ATATGCTAAG GTCTGAAGGA 
TGCGTTCAAG AATTTATTAA GAAGAACTCA GAGCCACCAA TGGATATTGT GATAGCTTGT 
CAACAATTAG CCGACGGACT AGTTGACAGA CTAATTGAAT TGGAAGATAC AGATAACTCG 
CGAATGCTGG GTTGTATTAC CACACTTCAT TTACTGGCTA AAGTGCGGCC CCAACTGCTT 
GTTAAGCATG CTATAACAAT TGAACCATAT CTCAACATAA AATGTCATTC TGCCACAGCC 
GCCAAGTTCA TATGTGCAGT TGCAGATATT CTCGAAAAAG TAGTTCCTCT GGTTAATAAT 
GCCAGTGAAT CCTTTTTAGC ATCGTTGGAA GAGCATTTAA TGTTATTGGT TGTTTCACGA 
AATCAAGCCG AAGTTACTAG CTGTGTTTCT TGCTTGGGAG CACTTGTGAA TAAGATTACA 
CATAACTTTA AATTGATCCG CGACTGTTTT CAAAAATTTT ATCGGGTTCT TGATGTGTCT 
CGAAGTCAAG TAATACAAGG AAATAACAGC GTAGATAATA TATACACTCC CAGCTTTCGG 
CGAAGTTTGT TTACGATCGG AATACTAATG CGATATTTCG ACTTTAAGTC TCCTATTGCG 
TTAGGTGAAA CAAATGATGG ACTTCCTGTT TCTATATGTG AGGATGTATT CCATTGCTTG 
ATGTTCTTCT GCCGTTGTAC TAATCAAGAA ATTCGAAAGC AAGCACTTAT TTCGCTTGGA 
TCATTTTGTG TACTAAACGA TGGCTACCTT ACAAGATCCG AACTTAAAAA TTTGTATTGC 
GAAATTTTAA GTTCAATAGC CAACGACGCC GGCTTTAAAA TAATCTGCAT GCGAAATATT 
TGGATTTATC TAACGGAATC TGAAATGTTT ATGCACAATA AGGAAAAAGA ATGGGAAAAA 
CAATCAAAAC ACGAAGATCT TAAGGAAATG AACGACGTTT CGTCTGGCAT GGCAAGTCGA 
ATAATTCAAC TATATTTAGA AGAAATTCTT GAATGCTTTC TTAATCGGGA TGACACAGTT 
CGGTTATGGG CTGTCAAAGT AATACAGATT GTCCTCCGTC AAGGTTTGGT TCACCCTGTT 
AGAATGGTTC CGTATTTAAT TTGCCTGAGT ACTGATCATA GGATAGAGTC TGCTCATCGA 
GCTGATGCCC TGTTGAAAGA TATTGATAAA ACATACTCTG GATTTGTTAA TATGAAAGTG 
CAATTCGGGC TACAGCTTTG CTTTAAGCTA CAAAAAATTT TACAAATAAA CAACAGAGGG 
AAACTGGAAA TAATTAGAGG TTATGCGAGT CGTGGACCAG ATAATACAAC TACCGCTTTA 
AACGATTTTC TGTATACACT TCTTCGCACT ACTAAACCGC AAAGGCGTGC TTTGGTGCAA 
ACAGTTACAA AACAGTTTGA CGATCAGAAA ACATCATTAC AACAAATGTT ATATATTGCA 
GATAACCTGG CTTACTTTCC ATATGTAGTT CAAGATGAAC CTTTATATCT AATCCATCAA 
ATAGATCTTC TAATTTCTAT GGCTGGAACA CATTTACTTG CTACATTCAA GGAACATATA 
AAACCAAGCG ACAAGGAAGG AGATGTTTTA GAAGATGATG ATGACGTAGA AGATCCAGAG 
GTGTTGTTTA ATAGATTGCC CGAAGATTTG ACGGAAATTA TTAAATGTAT AACATCTGCT 
CAAGCATGTA TGCTTCTTTT AATACTGAAA GATCATCTGA AGGAAATGTA TGCGATTACT 
GACAGTAAAA TTTCAAGATA TTCACCTTCC GAACAAAAAT TATATGAAAA AGCCGTAACA 
CGTAAAAGTG TTAATGATTT TAACCCAAAA ACGACTATTG ATGTTATAAA GAAACAAATG 
TCGCAAGAGA AATTGAGTAC GGACATTAAT TTTACACTGA CTAAAGAGGA GAAATTGGAC 
CTTGTTGTTA AATACCTTGA TTTTAAGCAA CTGATGCTTA AATTGGATCC GGATGATGGA 
GACTCCGATG CTGATGAATC CCGCGACAAA ACAATGTTAA ATATCTCTGC TTCTTCGGAT 
GGAGTGGCAT TCTCTAGTTC AGCAAAAAAT TCTCACTCCG CTTGTGATGG ATACTCACTA 
ACAGTCACGG ATGTGGTTGA TGTCCCAATG TCTCATATTG CGAAGGCATC TATGTTGACT 
TCAAAACCTA GTGGTCGCAA AACAAATCCT GTGCGAACTA AAAAAAAAAG AAGAAAAATT 
GATTCTACGG ATGATGAAAC CAGTGATGCG GAATATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nipped-B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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