Cht10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cht10
Entrez Gene ID	3355116
Full Name	Chitinase 10
Synonyms	BcDNA:LP04696,CG17682,CG18140,CG18140-PA3,CHT1,CHT3,Cht1,Cht3,DmCHT1,DmCHT10,DmCHT3,DmCht1,DmCht10,DmCht3,Dmel\CG18140
General protein information	Preferred Names Chitinase 10 Names CG18140 gene product from transcript CG18140-RA CG18140-PA CG18140-PB CG18140-PC Cht10-PA Cht10-PB Cht10-PC chitinase chitinase 1 chitinase 10 chitinase 3 chitinase-1 chitinase-3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273769.1	NP_001260698.1	chitinase 10, isoform C
NM_001042957.2	NP_001036422.1	chitinase 10, isoform A
NM_001273768.1	NP_001260697.1	chitinase 10, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Cht3	NP_001260698.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009022	XP_001238192.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cht10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cht10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15554	NM_001042957.2 Latest version!	Drosophila melanogaster chitinase 10, transcript variant A (Cht10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFa15554	NM_001273768.1 Latest version!	Drosophila melanogaster chitinase 10, transcript variant B (Cht10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFa15554	NM_001273769.1 Latest version!	Drosophila melanogaster chitinase 10, transcript variant C (Cht10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cht10 ( NM_001273769.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260698.1 Drosophila melanogaster chitinase 10, transcript variant C (Cht10), mRNA.

Price & Availability↑

CloneID	OFa15554
Clone ID Related Accession (Same CDS sequence)	NM_001273769.1 , NM_001042957.2 , NM_001273768.1
Accession Version	NM_001273769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chitinase 10, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCCGT ATGTCCCAAG TGTGCTTGTG GTGGTAGTCT TGGCTATATC TGTAAAAGCC 
CACATTCGCG TTGATAAACT GCAGATCCAT TCGGAATCCA ACTATAAACC TGCATTCATA 
CGTTCTGCGG TTGAGAGCAT TCCTTTGGAT ATTGATGCCA TACTCCTACT CCGTAACAGC 
ACAAAACCTG AGTTTGGGGA CGCTTTCCTA CCGCTGAGGA GTGCAGTGGA GAGTATTCCC 
AAGCTTTATC AGAGCAACAC GTCTAGGTTC AGAAAGAACC CTGATGTACG TTCTTTTGTG 
CGTGATTCTG TAGAGTCATT GCCAAATGAT GAAGACGGAC TGGAACACAT AGAAATTTTC 
ACTAAATATT ATAACAGTGT TAGCGATGTT GCAGGAGTTG ATCTAAAAAG CAAAAAGTTA 
ACACATGTGA ATTTAGATCC TGGGACCATT TCCGATAAGT ACGCAGCTTT TATTGCCCGC 
GGAAACTCAG AAGACTATTA TCCGAAGTCA TACCGAGCCG GCTCGCCGCA TCAGGAGTTG 
CTTAAAATGA TGAACATCGA ACACACCGAT AAACTTGATC GGTACTCTGA CCTGCAACAC 
CAGCAGTCCT ACGGAGCTTT GGGGTTGGCA AACAGGAATG ATCTCAAAAT GACTAAAAAA 
GTTCTTTGTT ACATGTCGAA TTGGGCATTC TACCGTAGTG GTGAGGCTCA TTTTGTGCCA 
GAGCAGATTG ATCCTAATCT ATGCTCTGCT ATTATCTATT CTTTTGCCTC CCTGGATCCT 
GATCACCTTA CAATTCGAGA ATTCGATTCA TGGGTGGACT TGGATAACCA ATACTACCGT 
CGAGTGACAT CGCTGGGTGT GCCTGTACTA ATTGCATTGG GTGGATGGAC GGACTCTAGC 
GGTTCCAAAT ACTCCCGATT AGTCAGTGAC AATCTTAAGC GACGTGTTTT TATATCCAGT 
GTGAGCAGCT TTTTACTACG TCATGGATTC AGCGGGCTCC ACCTAGACTG GAACTACCCG 
AAGTGCTGGC AGAGCGATTG CTCAAGGGGA CCTGTGACTG ACAGGCCAAA CCTGACTAAG 
CTTCTTCGTG AACTACGCAC CGAGTTTCAA AGTGTTGATC CGAAATTTCA GTTGGGAGTG 
GCCATCTCTG GCTACAAGGA GATTATAAAG GAGGCTTATG ATTTTCCCGC TTTGTCAGAC 
ATTGTAGACT ATATGACAGT AATGACATAC GATTACCACG GAGCTTGGGA ACAGAAAACA 
GGTCATGTGA GTCCACTTTA CGGCTTATCA TCGGACACTT ATCCTCAGTA CAATACAAAC 
TATACCATGC AGTTACTCCT AAAAATGGGA GCGCGAAGAG AAAAACTTGT ATTGAGCATT 
CCGTTTTACG GCCAGAGTTT TACATTGGCA ACCGCCCACC AAATTCTTGC AGGCCCAGGA 
GTGGCAGCCT CTGGACCTGG AGATGCCGGT GAATTGACAA AGCAACCTGG AATGCTAGCT 
TACTACGAGA TTTGTCAGCG CCTAACAAAG TTTAATTGGA TAAGTGATCG GAATTTAAAC 
GTGATATTCG GTCCATTCGC TATGCTAAAC GACCAGTGGG TAGGATATGA AGACCCCACC 
AGTGCCCAAG CCAAAGCTCG ATATGCGGCT AACAACAACT TTGCCGGAGT GGCAGCCTGG 
ACCATTGACC TTGATGACTT CCGAAACCTA TGTTGCAATG AATCTTATCC TTTACTTAGG 
GCCATTAATA GAGCATTGGG TCGCTTGGAC TCGGAGCCGC CAACTCAACC GAACTGTAAG 
CGTCCTACAT TATTGGCGAC ACCAGTGCCG CCTCAAATGA CAACAATTAG TAGCGATGGT 
TCTGGGGGAT TGGGACAAAA TCACGATCAT ACAACTTCCT TGCCAAGCGG GCAGATCAGC 
AGTAGTCCTG TAAGTACCAC TATCACGAGC ACCTTCCCCT GGTGGAGTTC AACTACAAAA 
CGTCCGCGCG AGCCGACTAA GACTACAGCA CAACCAACTC ACACCACAAT CCTCATTCCA 
GCAGGGATCA ACCCAGTGGT ACAGCCCTCG AATTGCAAAA GCGGCGAGTT CTTCGCTGAC 
TCAAATAATT GTAACGCTTA CTATCACTGT TTTTTTGCGG GAGAGTTACA GCAACAGTTT 
TGTCCAAGCG GTCTTCACTG GAACAACGAA GCTAAAGGAT GTGACTGGCC GTCGTCCGCC 
CAATGCTCAC TAAAACTCGA TCAACACCTA AGTACTTCTT ATCCAAACCC GATTCAAACG 
TCTAAAAAGC CGGAGACGAC TTTGAAACCT AATAAGAAAC CGTCAGAGAT TTCCACACAT 
CATCAGGTAA ATTCTACCTC AAGCCGACCA CAATATATGC GTCCAACTAT ATTGGAGTGC 
ACTGAAGGTG ATTACTACCC ACACAGAAAC TGCCGGAAAT ATTACATCTG CGTTAATAAG 
GCACTGGTAC CCAGTGAATG TGGCGGAGAC TTGCATTGGG ACGGTATCAA AAAACTTTGT 
GACTGGCCTG AGAACGTACA ATGCGTAACT AGCAAAAAAT ATTTAAAAAT AATCAAATCG 
AGTAGCGCCA ACGAAGAAGA CCCGTGCAAA GGCGAAAAGA GAGTACCCTA TCCAGGAAAC 
TGTTCGAAAT ATCTCTTCTG CCTATGGAAC CGCCTTCAGG CTAGTGACTG TCCACCAGGG 
CTGCACTATA ATGAAAGAAT TGGGAACTGC GATTGGCCAG CAGCTGCAAA ATGCAATCCA 
AAAGGTAGTG AGAGCAGCGA AGAGGCAGAG CTGAACGCTA TGCCGAAGCC TCCGACTCCA 
CAAACCCCCA GTAGTCATTT GCGTCCAACG TACCCCACAG AAAAACCAGT TCCCAAACCT 
CGTGATAGTC ACTACAAAGT TATTTGCTAC TTTACAAACT GGGCCTGGTA CCGCAAAGGA 
ATAGGTCGCT TTACTCCCGA CGACATAAAC ACGGAGCTGT GCACTCACGT AATTTATGGG 
TTTGCAGTGT TAGACTATTC CGAACTTGTA CTACGAACAC ATGATTCCTG GGCTGACGTT 
GAAAACAACT TTTACACAAG GGTCACCAGC CTCAAAAGCA AGGGCATCAA GGTTAGCTTA 
GCACTTGGTG GATGGAACGA TTCGCAAGGC GATAAGTACA GCCGCCTAGT TCGAAGTCCA 
ATGGCCCGGT CCCGTTTTGT GCGCCACGCC CTCGAGTTTA TTGAAAAATA CGGCTTTGAG 
GGCCTTGATT TAGATTGGGA GTACCCAGTC TGCTGGCAAA CGGAGTGCAA CAAAGGGTCT 
ACCGAGGAAA AGGACGGTTT CACTGCCTGG GTACAGGAGC TTTCTGAAGC GTTTCGCCCT 
CGTGGACTTA TGTTGTCCAC TGCTGTCTCG CCTAGCAGGA AAATCATTGA TGCCGGATAC 
GACATCCCTC AGCTCTCTCG CTATTTTGAT TGGATCGCTG TCATGACATA TGATTTTCAT 
GGACATTGGG ATAAAAAAAC AGGACATGTG GCACCGCTGT ATCATCATCC CGATGATGAC 
TTTGAATATT TTAACGTGAA CTATTCTATA AACTATTGGA TGGAAAAAGG AGCACCATCA 
CAAAAATTAG TAATGGGTAT TCCGCTATAC GGCCAGTCGT TCACTTTGGA AAATACCAAC 
AGCAGTGGCT TAAATGCCAA AGCCCCTGCA CCAGGTGAAG CTGGAGAGTT CACCAGAGCA 
GCTGGATTTC TTGCTTACTA TGAGATTTGT GAACGAGTAA ATCGCCAAGG CTGGCAGGTA 
GTGCATGATG AATTTGGGCG CATGGGACCT TATGCTTACA AGGGAACTCA GTGGGTGTCC 
TACGATAGCC CCGATATGGT CAGGAAAAAA TCACTACTGG TGAGGTCATT GAAACTGGGC 
GGTGGAATGG TTTGGGCTCT TGATCTGGAT GACTTTAAAA ATCGGTGTGG AAATGGCGTG 
CATCCTTTGC TCACTGAAAT CCATAACGTG CTTAAGGATC CACCTAGTTT AATGGAAATA 
CCTGGTCCTA TCGAAACGAC TCCTACAGAG TACCCAGGCA TGGAAGAAGA GATACATGAG 
TCTAATGGGG AGGGTCCCGA GGTGCAGCCG ATTGAAGCGG TGATGCAGAC CTGCGAAAAT 
GAAGGTGAAG AACATGAAGG TATTTTAGAC CCAAATCACG TCCTTGAGGA GGAAAACATA 
GAAGCTACTG AAATGGCCAC GGAATTTAAA ATCATTTGCT ACTTTACCAA CTGGGCGTGG 
TACCGTCAAG GTGGTGGTAA GTTCCTGCCG GAAGATATTG ATTCGGATCT GTGTACCCAC 
ATAATTTATG GCTTTGCCGT GTTGAGTCGG GACAACCTTA CGATTCAGCC TCATGACTCT 
TGGGCGGACT TAGACAACAA GTTTTATGAG CGGATTGTGG CCTACCGCAA GAAAGGCGCT 
AAAGTAACTG TGGCAATTGG CGGTTGGAAT GATTCTGCTG GCGATAAGTA CTCCCGACTT 
GTAAGGAATC CAGAGGCAAG ATCTCGGTTT ATCCGTAATG TCTTGGATTT TATAGAGGAA 
TATAATTTCG ACGGCTTGGA CCTGGATTGG GAGTACCCCG TTTGCTGGCA GGTGGATTGC 
AAGAAAGGGA CGGCAGAAGA GAAGATTGGA TTCTCAGCTT TGGTGCGGGA GCTTTTTTAT 
GCCTTCCAGC CCAGGGGATT AATTCTGTCA GCTGCCGTGT CACCTAACAA GAAGGTAATC 
GATGCTGGAT ACGAAGTTGC GGAGCTATCC CATTACTTTT CTTGGATATC TGTGATGGCC 
TATGACTACC ACGGTCAGTG GGATAAGAAG ACTGGCCATG TAGCTCCAAT GTACAGCCAC 
CCAGAAGGAA CAGCAAATTT TAACGCCAAT TTTTCCATGA ACTACTGGAT CTCGATGGGA 
GCTGACCGGC GAAAGTTAGT AATGGGAATA CCTCTGTACG GCCAGTCATT CTCTCTGGCT 
GAGACTACTA AGCACCAACT AAATGCTCCC ACGTATGGTG GCGGTGAAGC TGGAGAGGCT 
ACAAGAGCCC GAGGATTTTT GGCATACTAT GAAATATGCT TGAAAATCCG ACATCATAGG 
TGGAACGTTG TAAGGGATAC CAAGGGTCGA ATAGGACCCT TCGCATATCA TGGGGATCAG 
TGGGTTTCGT TCGATGATGT ACCCATGATT CGGCATAAAA GCGAGTACAT CAAGGCGATG 
GGTCTTGGTG GAGCTATGAT ATGGGCTCTA GACTTGGATG ACTTCAAAAA TGTGTGCGAA 
TGCGAGTCAT ATCCCTTGCT TAAAGCTATT AATCGTGTAC TCAGAGGTTT CGGGGGTCCC 
CAACCGAAGT GCTTACTTGA AAACCCCAAA AGCACAATGA AGCCAAACAT CAAACCGCCT 
TTCAGGCCAA CAATAAATGC CCCATCTGGA CCTAATCTTG ATTCTCCTAC CCAAAATGTA 
TCCTTAAATG TCCTGTCCAT CAAATGCCAC AGCAAAAACT ATTTGGCGCA CGAATGGGAT 
TGCACTAAGT ACTATATCTG CGAACATGGA ACGTACGTCG AGCGAAGCTG TCCTTTGGGA 
CTTCAATGGA ATAAAACCTA CTGCGACTGG CCTACCAATG TTCAGTCCTC ATTGGGATCA 
AACCAGAGAA CTCAGGAGCC AGCGGTCCAT CGCCCCAATC CTACAAGTGT CATTACAGAG 
CCCCCAATCA TCAATAACTC ATACAAAGTA GTCTGTTACT TTACGAGCTG GGCATGGTAC 
CGTTCGTCAC AGGGCAAGTT TGTGCCTGAA GATATAGACG CCAACTTGTG CACCCACCTC 
ATTTACGGCT TCGCGGTGCT TGATAGCAAG TCGTTAACCA TTAAGACGCA TGACTCCTGG 
ACCGATATAG ACAACCGTTT CTATGAGCGA GTTGTAGAAT ACAAGCAAAG AGGCCTTCGA 
GTCATGTTAG CCATTGGTGG ATGGAATGAC TCGTTGGGCA GCAAGTACGC ACGTCTTGTT 
CTTAATTCAC AGTCCAGGAG GCGCTTTGTC GCAAGTGTGA TAAGTTTTCT AGAACAACAC 
GGTTTTGAGG GTCTGGACTT AGCCTGGGAG TTTCCTGTAT GCTGGCAAGT TAACTGCAAC 
AGAGGTAATC CCACGGAAAA GGATGGATTT GTTGCTCTTG TCAAAGAACT GTCGGAAGCC 
TTTAAGGAAA ACGGTCTTAT TCTTTCGGCA GCAGTCTCGC CCAGTAAAAT GGTTATTGAT 
GCCGGGTACA ATGTGTTTGA GCTCTCTCCG TACTTTGACT GGGTGGCCGT CATGACGTAC 
GACTTTCACG GTCACTGGGA TATGCGCACT GGCCAAATAG CCCCACTCTT TCACAGAGGG 
GGTGACGAAA ACCTATACCT TAACGGAAAC TTCAGCATCC ATTACTGGTT GGAAAGGGGT 
ATTCCAAACG ACAAGCTGGT TATGGGAATG CCAATGTACG GACAGACGTT TACGCTAGCT 
GACCAAAATC GCCGGTCACT AAATGATAAA ACAGTGGGTC CGGGGAAGGC AGGAACCTTT 
ACTAGAGCGG ATGGATTCTT GGCGTACTAT GAAATTTGCG AAAAAGTCGT CAACGATGAC 
TGGAAAGTGG TGAGGGATGA AGAAGGTATA TTCGGATCCT ATGCATATAG TGGCAACCAG 
TGGATATCAT ACGATGATGT CACAACTATT CGAAGAAAAT CTCAGTTTAT TAAGAGCTTG 
CAGCTAGGTG GCGGCATGAT TTGGGCCCTT GATTTAGATG ACTTTCGTGG ACTTTGCGGT 
TGCGGCAAGC ACCCATTACT CAGGACTCTT AGTCAGGAGC TCCTAGGCAT TCCTGGACAA 
AAGGCTAAGG ACTGCATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260698.1
CDS	208..7068
Translation	MHPYVPSVLVVVVLAISVKAHIRVDKLQIHSESNYKPAFIRSAVESIPLDIDAILLLRNSTKPEFGDAFLPLRSAVESIPKLYQSNTSRFRKNPDVRSFVRDSVESLPNDEDGLEHIEIFTKYYNSVSDVAGVDLKSKKLTHVNLDPGTISDKYAAFIARGNSEDYYPKSYRAGSPHQELLKMMNIEHTDKLDRYSDLQHQQSYGALGLANRNDLKMTKKVLCYMSNWAFYRSGEAHFVPEQIDPNLCSAIIYSFASLDPDHLTIREFDSWVDLDNQYYRRVTSLGVPVLIALGGWTDSSGSKYSRLVSDNLKRRVFISSVSSFLLRHGFSGLHLDWNYPKCWQSDCSRGPVTDRPNLTKLLRELRTEFQSVDPKFQLGVAISGYKEIIKEAYDFPALSDIVDYMTVMTYDYHGAWEQKTGHVSPLYGLSSDTYPQYNTNYTMQLLLKMGARREKLVLSIPFYGQSFTLATAHQILAGPGVAASGPGDAGELTKQPGMLAYYEICQRLTKFNWISDRNLNVIFGPFAMLNDQWVGYEDPTSAQAKARYAANNNFAGVAAWTIDLDDFRNLCCNESYPLLRAINRALGRLDSEPPTQPNCKRPTLLATPVPPQMTTISSDGSGGLGQNHDHTTSLPSGQISSSPVSTTITSTFPWWSSTTKRPREPTKTTAQPTHTTILIPAGINPVVQPSNCKSGEFFADSNNCNAYYHCFFAGELQQQFCPSGLHWNNEAKGCDWPSSAQCSLKLDQHLSTSYPNPIQTSKKPETTLKPNKKPSEISTHHQVNSTSSRPQYMRPTILECTEGDYYPHRNCRKYYICVNKALVPSECGGDLHWDGIKKLCDWPENVQCVTSKKYLKIIKSSSANEEDPCKGEKRVPYPGNCSKYLFCLWNRLQASDCPPGLHYNERIGNCDWPAAAKCNPKGSESSEEAELNAMPKPPTPQTPSSHLRPTYPTEKPVPKPRDSHYKVICYFTNWAWYRKGIGRFTPDDINTELCTHVIYGFAVLDYSELVLRTHDSWADVENNFYTRVTSLKSKGIKVSLALGGWNDSQGDKYSRLVRSPMARSRFVRHALEFIEKYGFEGLDLDWEYPVCWQTECNKGSTEEKDGFTAWVQELSEAFRPRGLMLSTAVSPSRKIIDAGYDIPQLSRYFDWIAVMTYDFHGHWDKKTGHVAPLYHHPDDDFEYFNVNYSINYWMEKGAPSQKLVMGIPLYGQSFTLENTNSSGLNAKAPAPGEAGEFTRAAGFLAYYEICERVNRQGWQVVHDEFGRMGPYAYKGTQWVSYDSPDMVRKKSLLVRSLKLGGGMVWALDLDDFKNRCGNGVHPLLTEIHNVLKDPPSLMEIPGPIETTPTEYPGMEEEIHESNGEGPEVQPIEAVMQTCENEGEEHEGILDPNHVLEEENIEATEMATEFKIICYFTNWAWYRQGGGKFLPEDIDSDLCTHIIYGFAVLSRDNLTIQPHDSWADLDNKFYERIVAYRKKGAKVTVAIGGWNDSAGDKYSRLVRNPEARSRFIRNVLDFIEEYNFDGLDLDWEYPVCWQVDCKKGTAEEKIGFSALVRELFYAFQPRGLILSAAVSPNKKVIDAGYEVAELSHYFSWISVMAYDYHGQWDKKTGHVAPMYSHPEGTANFNANFSMNYWISMGADRRKLVMGIPLYGQSFSLAETTKHQLNAPTYGGGEAGEATRARGFLAYYEICLKIRHHRWNVVRDTKGRIGPFAYHGDQWVSFDDVPMIRHKSEYIKAMGLGGAMIWALDLDDFKNVCECESYPLLKAINRVLRGFGGPQPKCLLENPKSTMKPNIKPPFRPTINAPSGPNLDSPTQNVSLNVLSIKCHSKNYLAHEWDCTKYYICEHGTYVERSCPLGLQWNKTYCDWPTNVQSSLGSNQRTQEPAVHRPNPTSVITEPPIINNSYKVVCYFTSWAWYRSSQGKFVPEDIDANLCTHLIYGFAVLDSKSLTIKTHDSWTDIDNRFYERVVEYKQRGLRVMLAIGGWNDSLGSKYARLVLNSQSRRRFVASVISFLEQHGFEGLDLAWEFPVCWQVNCNRGNPTEKDGFVALVKELSEAFKENGLILSAAVSPSKMVIDAGYNVFELSPYFDWVAVMTYDFHGHWDMRTGQIAPLFHRGGDENLYLNGNFSIHYWLERGIPNDKLVMGMPMYGQTFTLADQNRRSLNDKTVGPGKAGTFTRADGFLAYYEICEKVVNDDWKVVRDEEGIFGSYAYSGNQWISYDDVTTIRRKSQFIKSLQLGGGMIWALDLDDFRGLCGCGKHPLLRTLSQELLGIPGQKAKDCI

Target ORF information:

RefSeq Version	NM_001273769.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chitinase 10, transcript variant C (Cht10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273769.1

ORF Insert Sequence:

ATGCATCCGT ATGTCCCAAG TGTGCTTGTG GTGGTAGTCT TGGCTATATC TGTAAAAGCC 
CACATTCGCG TTGATAAACT GCAGATCCAT TCGGAATCCA ACTATAAACC TGCATTCATA 
CGTTCTGCGG TTGAGAGCAT TCCTTTGGAT ATTGATGCCA TACTCCTACT CCGTAACAGC 
ACAAAACCTG AGTTTGGGGA CGCTTTCCTA CCGCTGAGGA GTGCAGTGGA GAGTATTCCC 
AAGCTTTATC AGAGCAACAC GTCTAGGTTC AGAAAGAACC CTGATGTACG TTCTTTTGTG 
CGTGATTCTG TAGAGTCATT GCCAAATGAT GAAGACGGAC TGGAACACAT AGAAATTTTC 
ACTAAATATT ATAACAGTGT TAGCGATGTT GCAGGAGTTG ATCTAAAAAG CAAAAAGTTA 
ACACATGTGA ATTTAGATCC TGGGACCATT TCCGATAAGT ACGCAGCTTT TATTGCCCGC 
GGAAACTCAG AAGACTATTA TCCGAAGTCA TACCGAGCCG GCTCGCCGCA TCAGGAGTTG 
CTTAAAATGA TGAACATCGA ACACACCGAT AAACTTGATC GGTACTCTGA CCTGCAACAC 
CAGCAGTCCT ACGGAGCTTT GGGGTTGGCA AACAGGAATG ATCTCAAAAT GACTAAAAAA 
GTTCTTTGTT ACATGTCGAA TTGGGCATTC TACCGTAGTG GTGAGGCTCA TTTTGTGCCA 
GAGCAGATTG ATCCTAATCT ATGCTCTGCT ATTATCTATT CTTTTGCCTC CCTGGATCCT 
GATCACCTTA CAATTCGAGA ATTCGATTCA TGGGTGGACT TGGATAACCA ATACTACCGT 
CGAGTGACAT CGCTGGGTGT GCCTGTACTA ATTGCATTGG GTGGATGGAC GGACTCTAGC 
GGTTCCAAAT ACTCCCGATT AGTCAGTGAC AATCTTAAGC GACGTGTTTT TATATCCAGT 
GTGAGCAGCT TTTTACTACG TCATGGATTC AGCGGGCTCC ACCTAGACTG GAACTACCCG 
AAGTGCTGGC AGAGCGATTG CTCAAGGGGA CCTGTGACTG ACAGGCCAAA CCTGACTAAG 
CTTCTTCGTG AACTACGCAC CGAGTTTCAA AGTGTTGATC CGAAATTTCA GTTGGGAGTG 
GCCATCTCTG GCTACAAGGA GATTATAAAG GAGGCTTATG ATTTTCCCGC TTTGTCAGAC 
ATTGTAGACT ATATGACAGT AATGACATAC GATTACCACG GAGCTTGGGA ACAGAAAACA 
GGTCATGTGA GTCCACTTTA CGGCTTATCA TCGGACACTT ATCCTCAGTA CAATACAAAC 
TATACCATGC AGTTACTCCT AAAAATGGGA GCGCGAAGAG AAAAACTTGT ATTGAGCATT 
CCGTTTTACG GCCAGAGTTT TACATTGGCA ACCGCCCACC AAATTCTTGC AGGCCCAGGA 
GTGGCAGCCT CTGGACCTGG AGATGCCGGT GAATTGACAA AGCAACCTGG AATGCTAGCT 
TACTACGAGA TTTGTCAGCG CCTAACAAAG TTTAATTGGA TAAGTGATCG GAATTTAAAC 
GTGATATTCG GTCCATTCGC TATGCTAAAC GACCAGTGGG TAGGATATGA AGACCCCACC 
AGTGCCCAAG CCAAAGCTCG ATATGCGGCT AACAACAACT TTGCCGGAGT GGCAGCCTGG 
ACCATTGACC TTGATGACTT CCGAAACCTA TGTTGCAATG AATCTTATCC TTTACTTAGG 
GCCATTAATA GAGCATTGGG TCGCTTGGAC TCGGAGCCGC CAACTCAACC GAACTGTAAG 
CGTCCTACAT TATTGGCGAC ACCAGTGCCG CCTCAAATGA CAACAATTAG TAGCGATGGT 
TCTGGGGGAT TGGGACAAAA TCACGATCAT ACAACTTCCT TGCCAAGCGG GCAGATCAGC 
AGTAGTCCTG TAAGTACCAC TATCACGAGC ACCTTCCCCT GGTGGAGTTC AACTACAAAA 
CGTCCGCGCG AGCCGACTAA GACTACAGCA CAACCAACTC ACACCACAAT CCTCATTCCA 
GCAGGGATCA ACCCAGTGGT ACAGCCCTCG AATTGCAAAA GCGGCGAGTT CTTCGCTGAC 
TCAAATAATT GTAACGCTTA CTATCACTGT TTTTTTGCGG GAGAGTTACA GCAACAGTTT 
TGTCCAAGCG GTCTTCACTG GAACAACGAA GCTAAAGGAT GTGACTGGCC GTCGTCCGCC 
CAATGCTCAC TAAAACTCGA TCAACACCTA AGTACTTCTT ATCCAAACCC GATTCAAACG 
TCTAAAAAGC CGGAGACGAC TTTGAAACCT AATAAGAAAC CGTCAGAGAT TTCCACACAT 
CATCAGGTAA ATTCTACCTC AAGCCGACCA CAATATATGC GTCCAACTAT ATTGGAGTGC 
ACTGAAGGTG ATTACTACCC ACACAGAAAC TGCCGGAAAT ATTACATCTG CGTTAATAAG 
GCACTGGTAC CCAGTGAATG TGGCGGAGAC TTGCATTGGG ACGGTATCAA AAAACTTTGT 
GACTGGCCTG AGAACGTACA ATGCGTAACT AGCAAAAAAT ATTTAAAAAT AATCAAATCG 
AGTAGCGCCA ACGAAGAAGA CCCGTGCAAA GGCGAAAAGA GAGTACCCTA TCCAGGAAAC 
TGTTCGAAAT ATCTCTTCTG CCTATGGAAC CGCCTTCAGG CTAGTGACTG TCCACCAGGG 
CTGCACTATA ATGAAAGAAT TGGGAACTGC GATTGGCCAG CAGCTGCAAA ATGCAATCCA 
AAAGGTAGTG AGAGCAGCGA AGAGGCAGAG CTGAACGCTA TGCCGAAGCC TCCGACTCCA 
CAAACCCCCA GTAGTCATTT GCGTCCAACG TACCCCACAG AAAAACCAGT TCCCAAACCT 
CGTGATAGTC ACTACAAAGT TATTTGCTAC TTTACAAACT GGGCCTGGTA CCGCAAAGGA 
ATAGGTCGCT TTACTCCCGA CGACATAAAC ACGGAGCTGT GCACTCACGT AATTTATGGG 
TTTGCAGTGT TAGACTATTC CGAACTTGTA CTACGAACAC ATGATTCCTG GGCTGACGTT 
GAAAACAACT TTTACACAAG GGTCACCAGC CTCAAAAGCA AGGGCATCAA GGTTAGCTTA 
GCACTTGGTG GATGGAACGA TTCGCAAGGC GATAAGTACA GCCGCCTAGT TCGAAGTCCA 
ATGGCCCGGT CCCGTTTTGT GCGCCACGCC CTCGAGTTTA TTGAAAAATA CGGCTTTGAG 
GGCCTTGATT TAGATTGGGA GTACCCAGTC TGCTGGCAAA CGGAGTGCAA CAAAGGGTCT 
ACCGAGGAAA AGGACGGTTT CACTGCCTGG GTACAGGAGC TTTCTGAAGC GTTTCGCCCT 
CGTGGACTTA TGTTGTCCAC TGCTGTCTCG CCTAGCAGGA AAATCATTGA TGCCGGATAC 
GACATCCCTC AGCTCTCTCG CTATTTTGAT TGGATCGCTG TCATGACATA TGATTTTCAT 
GGACATTGGG ATAAAAAAAC AGGACATGTG GCACCGCTGT ATCATCATCC CGATGATGAC 
TTTGAATATT TTAACGTGAA CTATTCTATA AACTATTGGA TGGAAAAAGG AGCACCATCA 
CAAAAATTAG TAATGGGTAT TCCGCTATAC GGCCAGTCGT TCACTTTGGA AAATACCAAC 
AGCAGTGGCT TAAATGCCAA AGCCCCTGCA CCAGGTGAAG CTGGAGAGTT CACCAGAGCA 
GCTGGATTTC TTGCTTACTA TGAGATTTGT GAACGAGTAA ATCGCCAAGG CTGGCAGGTA 
GTGCATGATG AATTTGGGCG CATGGGACCT TATGCTTACA AGGGAACTCA GTGGGTGTCC 
TACGATAGCC CCGATATGGT CAGGAAAAAA TCACTACTGG TGAGGTCATT GAAACTGGGC 
GGTGGAATGG TTTGGGCTCT TGATCTGGAT GACTTTAAAA ATCGGTGTGG AAATGGCGTG 
CATCCTTTGC TCACTGAAAT CCATAACGTG CTTAAGGATC CACCTAGTTT AATGGAAATA 
CCTGGTCCTA TCGAAACGAC TCCTACAGAG TACCCAGGCA TGGAAGAAGA GATACATGAG 
TCTAATGGGG AGGGTCCCGA GGTGCAGCCG ATTGAAGCGG TGATGCAGAC CTGCGAAAAT 
GAAGGTGAAG AACATGAAGG TATTTTAGAC CCAAATCACG TCCTTGAGGA GGAAAACATA 
GAAGCTACTG AAATGGCCAC GGAATTTAAA ATCATTTGCT ACTTTACCAA CTGGGCGTGG 
TACCGTCAAG GTGGTGGTAA GTTCCTGCCG GAAGATATTG ATTCGGATCT GTGTACCCAC 
ATAATTTATG GCTTTGCCGT GTTGAGTCGG GACAACCTTA CGATTCAGCC TCATGACTCT 
TGGGCGGACT TAGACAACAA GTTTTATGAG CGGATTGTGG CCTACCGCAA GAAAGGCGCT 
AAAGTAACTG TGGCAATTGG CGGTTGGAAT GATTCTGCTG GCGATAAGTA CTCCCGACTT 
GTAAGGAATC CAGAGGCAAG ATCTCGGTTT ATCCGTAATG TCTTGGATTT TATAGAGGAA 
TATAATTTCG ACGGCTTGGA CCTGGATTGG GAGTACCCCG TTTGCTGGCA GGTGGATTGC 
AAGAAAGGGA CGGCAGAAGA GAAGATTGGA TTCTCAGCTT TGGTGCGGGA GCTTTTTTAT 
GCCTTCCAGC CCAGGGGATT AATTCTGTCA GCTGCCGTGT CACCTAACAA GAAGGTAATC 
GATGCTGGAT ACGAAGTTGC GGAGCTATCC CATTACTTTT CTTGGATATC TGTGATGGCC 
TATGACTACC ACGGTCAGTG GGATAAGAAG ACTGGCCATG TAGCTCCAAT GTACAGCCAC 
CCAGAAGGAA CAGCAAATTT TAACGCCAAT TTTTCCATGA ACTACTGGAT CTCGATGGGA 
GCTGACCGGC GAAAGTTAGT AATGGGAATA CCTCTGTACG GCCAGTCATT CTCTCTGGCT 
GAGACTACTA AGCACCAACT AAATGCTCCC ACGTATGGTG GCGGTGAAGC TGGAGAGGCT 
ACAAGAGCCC GAGGATTTTT GGCATACTAT GAAATATGCT TGAAAATCCG ACATCATAGG 
TGGAACGTTG TAAGGGATAC CAAGGGTCGA ATAGGACCCT TCGCATATCA TGGGGATCAG 
TGGGTTTCGT TCGATGATGT ACCCATGATT CGGCATAAAA GCGAGTACAT CAAGGCGATG 
GGTCTTGGTG GAGCTATGAT ATGGGCTCTA GACTTGGATG ACTTCAAAAA TGTGTGCGAA 
TGCGAGTCAT ATCCCTTGCT TAAAGCTATT AATCGTGTAC TCAGAGGTTT CGGGGGTCCC 
CAACCGAAGT GCTTACTTGA AAACCCCAAA AGCACAATGA AGCCAAACAT CAAACCGCCT 
TTCAGGCCAA CAATAAATGC CCCATCTGGA CCTAATCTTG ATTCTCCTAC CCAAAATGTA 
TCCTTAAATG TCCTGTCCAT CAAATGCCAC AGCAAAAACT ATTTGGCGCA CGAATGGGAT 
TGCACTAAGT ACTATATCTG CGAACATGGA ACGTACGTCG AGCGAAGCTG TCCTTTGGGA 
CTTCAATGGA ATAAAACCTA CTGCGACTGG CCTACCAATG TTCAGTCCTC ATTGGGATCA 
AACCAGAGAA CTCAGGAGCC AGCGGTCCAT CGCCCCAATC CTACAAGTGT CATTACAGAG 
CCCCCAATCA TCAATAACTC ATACAAAGTA GTCTGTTACT TTACGAGCTG GGCATGGTAC 
CGTTCGTCAC AGGGCAAGTT TGTGCCTGAA GATATAGACG CCAACTTGTG CACCCACCTC 
ATTTACGGCT TCGCGGTGCT TGATAGCAAG TCGTTAACCA TTAAGACGCA TGACTCCTGG 
ACCGATATAG ACAACCGTTT CTATGAGCGA GTTGTAGAAT ACAAGCAAAG AGGCCTTCGA 
GTCATGTTAG CCATTGGTGG ATGGAATGAC TCGTTGGGCA GCAAGTACGC ACGTCTTGTT 
CTTAATTCAC AGTCCAGGAG GCGCTTTGTC GCAAGTGTGA TAAGTTTTCT AGAACAACAC 
GGTTTTGAGG GTCTGGACTT AGCCTGGGAG TTTCCTGTAT GCTGGCAAGT TAACTGCAAC 
AGAGGTAATC CCACGGAAAA GGATGGATTT GTTGCTCTTG TCAAAGAACT GTCGGAAGCC 
TTTAAGGAAA ACGGTCTTAT TCTTTCGGCA GCAGTCTCGC CCAGTAAAAT GGTTATTGAT 
GCCGGGTACA ATGTGTTTGA GCTCTCTCCG TACTTTGACT GGGTGGCCGT CATGACGTAC 
GACTTTCACG GTCACTGGGA TATGCGCACT GGCCAAATAG CCCCACTCTT TCACAGAGGG 
GGTGACGAAA ACCTATACCT TAACGGAAAC TTCAGCATCC ATTACTGGTT GGAAAGGGGT 
ATTCCAAACG ACAAGCTGGT TATGGGAATG CCAATGTACG GACAGACGTT TACGCTAGCT 
GACCAAAATC GCCGGTCACT AAATGATAAA ACAGTGGGTC CGGGGAAGGC AGGAACCTTT 
ACTAGAGCGG ATGGATTCTT GGCGTACTAT GAAATTTGCG AAAAAGTCGT CAACGATGAC 
TGGAAAGTGG TGAGGGATGA AGAAGGTATA TTCGGATCCT ATGCATATAG TGGCAACCAG 
TGGATATCAT ACGATGATGT CACAACTATT CGAAGAAAAT CTCAGTTTAT TAAGAGCTTG 
CAGCTAGGTG GCGGCATGAT TTGGGCCCTT GATTTAGATG ACTTTCGTGG ACTTTGCGGT 
TGCGGCAAGC ACCCATTACT CAGGACTCTT AGTCAGGAGC TCCTAGGCAT TCCTGGACAA 
AAGGCTAAGG ACTGCATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cht10 ( NM_001042957.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036422.1 Drosophila melanogaster chitinase 10, transcript variant A (Cht10), mRNA.

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CloneID	OFa15554
Clone ID Related Accession (Same CDS sequence)	NM_001273769.1 , NM_001042957.2 , NM_001273768.1
Accession Version	NM_001042957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chitinase 10, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001042957.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCCGT ATGTCCCAAG TGTGCTTGTG GTGGTAGTCT TGGCTATATC TGTAAAAGCC 
CACATTCGCG TTGATAAACT GCAGATCCAT TCGGAATCCA ACTATAAACC TGCATTCATA 
CGTTCTGCGG TTGAGAGCAT TCCTTTGGAT ATTGATGCCA TACTCCTACT CCGTAACAGC 
ACAAAACCTG AGTTTGGGGA CGCTTTCCTA CCGCTGAGGA GTGCAGTGGA GAGTATTCCC 
AAGCTTTATC AGAGCAACAC GTCTAGGTTC AGAAAGAACC CTGATGTACG TTCTTTTGTG 
CGTGATTCTG TAGAGTCATT GCCAAATGAT GAAGACGGAC TGGAACACAT AGAAATTTTC 
ACTAAATATT ATAACAGTGT TAGCGATGTT GCAGGAGTTG ATCTAAAAAG CAAAAAGTTA 
ACACATGTGA ATTTAGATCC TGGGACCATT TCCGATAAGT ACGCAGCTTT TATTGCCCGC 
GGAAACTCAG AAGACTATTA TCCGAAGTCA TACCGAGCCG GCTCGCCGCA TCAGGAGTTG 
CTTAAAATGA TGAACATCGA ACACACCGAT AAACTTGATC GGTACTCTGA CCTGCAACAC 
CAGCAGTCCT ACGGAGCTTT GGGGTTGGCA AACAGGAATG ATCTCAAAAT GACTAAAAAA 
GTTCTTTGTT ACATGTCGAA TTGGGCATTC TACCGTAGTG GTGAGGCTCA TTTTGTGCCA 
GAGCAGATTG ATCCTAATCT ATGCTCTGCT ATTATCTATT CTTTTGCCTC CCTGGATCCT 
GATCACCTTA CAATTCGAGA ATTCGATTCA TGGGTGGACT TGGATAACCA ATACTACCGT 
CGAGTGACAT CGCTGGGTGT GCCTGTACTA ATTGCATTGG GTGGATGGAC GGACTCTAGC 
GGTTCCAAAT ACTCCCGATT AGTCAGTGAC AATCTTAAGC GACGTGTTTT TATATCCAGT 
GTGAGCAGCT TTTTACTACG TCATGGATTC AGCGGGCTCC ACCTAGACTG GAACTACCCG 
AAGTGCTGGC AGAGCGATTG CTCAAGGGGA CCTGTGACTG ACAGGCCAAA CCTGACTAAG 
CTTCTTCGTG AACTACGCAC CGAGTTTCAA AGTGTTGATC CGAAATTTCA GTTGGGAGTG 
GCCATCTCTG GCTACAAGGA GATTATAAAG GAGGCTTATG ATTTTCCCGC TTTGTCAGAC 
ATTGTAGACT ATATGACAGT AATGACATAC GATTACCACG GAGCTTGGGA ACAGAAAACA 
GGTCATGTGA GTCCACTTTA CGGCTTATCA TCGGACACTT ATCCTCAGTA CAATACAAAC 
TATACCATGC AGTTACTCCT AAAAATGGGA GCGCGAAGAG AAAAACTTGT ATTGAGCATT 
CCGTTTTACG GCCAGAGTTT TACATTGGCA ACCGCCCACC AAATTCTTGC AGGCCCAGGA 
GTGGCAGCCT CTGGACCTGG AGATGCCGGT GAATTGACAA AGCAACCTGG AATGCTAGCT 
TACTACGAGA TTTGTCAGCG CCTAACAAAG TTTAATTGGA TAAGTGATCG GAATTTAAAC 
GTGATATTCG GTCCATTCGC TATGCTAAAC GACCAGTGGG TAGGATATGA AGACCCCACC 
AGTGCCCAAG CCAAAGCTCG ATATGCGGCT AACAACAACT TTGCCGGAGT GGCAGCCTGG 
ACCATTGACC TTGATGACTT CCGAAACCTA TGTTGCAATG AATCTTATCC TTTACTTAGG 
GCCATTAATA GAGCATTGGG TCGCTTGGAC TCGGAGCCGC CAACTCAACC GAACTGTAAG 
CGTCCTACAT TATTGGCGAC ACCAGTGCCG CCTCAAATGA CAACAATTAG TAGCGATGGT 
TCTGGGGGAT TGGGACAAAA TCACGATCAT ACAACTTCCT TGCCAAGCGG GCAGATCAGC 
AGTAGTCCTG TAAGTACCAC TATCACGAGC ACCTTCCCCT GGTGGAGTTC AACTACAAAA 
CGTCCGCGCG AGCCGACTAA GACTACAGCA CAACCAACTC ACACCACAAT CCTCATTCCA 
GCAGGGATCA ACCCAGTGGT ACAGCCCTCG AATTGCAAAA GCGGCGAGTT CTTCGCTGAC 
TCAAATAATT GTAACGCTTA CTATCACTGT TTTTTTGCGG GAGAGTTACA GCAACAGTTT 
TGTCCAAGCG GTCTTCACTG GAACAACGAA GCTAAAGGAT GTGACTGGCC GTCGTCCGCC 
CAATGCTCAC TAAAACTCGA TCAACACCTA AGTACTTCTT ATCCAAACCC GATTCAAACG 
TCTAAAAAGC CGGAGACGAC TTTGAAACCT AATAAGAAAC CGTCAGAGAT TTCCACACAT 
CATCAGGTAA ATTCTACCTC AAGCCGACCA CAATATATGC GTCCAACTAT ATTGGAGTGC 
ACTGAAGGTG ATTACTACCC ACACAGAAAC TGCCGGAAAT ATTACATCTG CGTTAATAAG 
GCACTGGTAC CCAGTGAATG TGGCGGAGAC TTGCATTGGG ACGGTATCAA AAAACTTTGT 
GACTGGCCTG AGAACGTACA ATGCGTAACT AGCAAAAAAT ATTTAAAAAT AATCAAATCG 
AGTAGCGCCA ACGAAGAAGA CCCGTGCAAA GGCGAAAAGA GAGTACCCTA TCCAGGAAAC 
TGTTCGAAAT ATCTCTTCTG CCTATGGAAC CGCCTTCAGG CTAGTGACTG TCCACCAGGG 
CTGCACTATA ATGAAAGAAT TGGGAACTGC GATTGGCCAG CAGCTGCAAA ATGCAATCCA 
AAAGGTAGTG AGAGCAGCGA AGAGGCAGAG CTGAACGCTA TGCCGAAGCC TCCGACTCCA 
CAAACCCCCA GTAGTCATTT GCGTCCAACG TACCCCACAG AAAAACCAGT TCCCAAACCT 
CGTGATAGTC ACTACAAAGT TATTTGCTAC TTTACAAACT GGGCCTGGTA CCGCAAAGGA 
ATAGGTCGCT TTACTCCCGA CGACATAAAC ACGGAGCTGT GCACTCACGT AATTTATGGG 
TTTGCAGTGT TAGACTATTC CGAACTTGTA CTACGAACAC ATGATTCCTG GGCTGACGTT 
GAAAACAACT TTTACACAAG GGTCACCAGC CTCAAAAGCA AGGGCATCAA GGTTAGCTTA 
GCACTTGGTG GATGGAACGA TTCGCAAGGC GATAAGTACA GCCGCCTAGT TCGAAGTCCA 
ATGGCCCGGT CCCGTTTTGT GCGCCACGCC CTCGAGTTTA TTGAAAAATA CGGCTTTGAG 
GGCCTTGATT TAGATTGGGA GTACCCAGTC TGCTGGCAAA CGGAGTGCAA CAAAGGGTCT 
ACCGAGGAAA AGGACGGTTT CACTGCCTGG GTACAGGAGC TTTCTGAAGC GTTTCGCCCT 
CGTGGACTTA TGTTGTCCAC TGCTGTCTCG CCTAGCAGGA AAATCATTGA TGCCGGATAC 
GACATCCCTC AGCTCTCTCG CTATTTTGAT TGGATCGCTG TCATGACATA TGATTTTCAT 
GGACATTGGG ATAAAAAAAC AGGACATGTG GCACCGCTGT ATCATCATCC CGATGATGAC 
TTTGAATATT TTAACGTGAA CTATTCTATA AACTATTGGA TGGAAAAAGG AGCACCATCA 
CAAAAATTAG TAATGGGTAT TCCGCTATAC GGCCAGTCGT TCACTTTGGA AAATACCAAC 
AGCAGTGGCT TAAATGCCAA AGCCCCTGCA CCAGGTGAAG CTGGAGAGTT CACCAGAGCA 
GCTGGATTTC TTGCTTACTA TGAGATTTGT GAACGAGTAA ATCGCCAAGG CTGGCAGGTA 
GTGCATGATG AATTTGGGCG CATGGGACCT TATGCTTACA AGGGAACTCA GTGGGTGTCC 
TACGATAGCC CCGATATGGT CAGGAAAAAA TCACTACTGG TGAGGTCATT GAAACTGGGC 
GGTGGAATGG TTTGGGCTCT TGATCTGGAT GACTTTAAAA ATCGGTGTGG AAATGGCGTG 
CATCCTTTGC TCACTGAAAT CCATAACGTG CTTAAGGATC CACCTAGTTT AATGGAAATA 
CCTGGTCCTA TCGAAACGAC TCCTACAGAG TACCCAGGCA TGGAAGAAGA GATACATGAG 
TCTAATGGGG AGGGTCCCGA GGTGCAGCCG ATTGAAGCGG TGATGCAGAC CTGCGAAAAT 
GAAGGTGAAG AACATGAAGG TATTTTAGAC CCAAATCACG TCCTTGAGGA GGAAAACATA 
GAAGCTACTG AAATGGCCAC GGAATTTAAA ATCATTTGCT ACTTTACCAA CTGGGCGTGG 
TACCGTCAAG GTGGTGGTAA GTTCCTGCCG GAAGATATTG ATTCGGATCT GTGTACCCAC 
ATAATTTATG GCTTTGCCGT GTTGAGTCGG GACAACCTTA CGATTCAGCC TCATGACTCT 
TGGGCGGACT TAGACAACAA GTTTTATGAG CGGATTGTGG CCTACCGCAA GAAAGGCGCT 
AAAGTAACTG TGGCAATTGG CGGTTGGAAT GATTCTGCTG GCGATAAGTA CTCCCGACTT 
GTAAGGAATC CAGAGGCAAG ATCTCGGTTT ATCCGTAATG TCTTGGATTT TATAGAGGAA 
TATAATTTCG ACGGCTTGGA CCTGGATTGG GAGTACCCCG TTTGCTGGCA GGTGGATTGC 
AAGAAAGGGA CGGCAGAAGA GAAGATTGGA TTCTCAGCTT TGGTGCGGGA GCTTTTTTAT 
GCCTTCCAGC CCAGGGGATT AATTCTGTCA GCTGCCGTGT CACCTAACAA GAAGGTAATC 
GATGCTGGAT ACGAAGTTGC GGAGCTATCC CATTACTTTT CTTGGATATC TGTGATGGCC 
TATGACTACC ACGGTCAGTG GGATAAGAAG ACTGGCCATG TAGCTCCAAT GTACAGCCAC 
CCAGAAGGAA CAGCAAATTT TAACGCCAAT TTTTCCATGA ACTACTGGAT CTCGATGGGA 
GCTGACCGGC GAAAGTTAGT AATGGGAATA CCTCTGTACG GCCAGTCATT CTCTCTGGCT 
GAGACTACTA AGCACCAACT AAATGCTCCC ACGTATGGTG GCGGTGAAGC TGGAGAGGCT 
ACAAGAGCCC GAGGATTTTT GGCATACTAT GAAATATGCT TGAAAATCCG ACATCATAGG 
TGGAACGTTG TAAGGGATAC CAAGGGTCGA ATAGGACCCT TCGCATATCA TGGGGATCAG 
TGGGTTTCGT TCGATGATGT ACCCATGATT CGGCATAAAA GCGAGTACAT CAAGGCGATG 
GGTCTTGGTG GAGCTATGAT ATGGGCTCTA GACTTGGATG ACTTCAAAAA TGTGTGCGAA 
TGCGAGTCAT ATCCCTTGCT TAAAGCTATT AATCGTGTAC TCAGAGGTTT CGGGGGTCCC 
CAACCGAAGT GCTTACTTGA AAACCCCAAA AGCACAATGA AGCCAAACAT CAAACCGCCT 
TTCAGGCCAA CAATAAATGC CCCATCTGGA CCTAATCTTG ATTCTCCTAC CCAAAATGTA 
TCCTTAAATG TCCTGTCCAT CAAATGCCAC AGCAAAAACT ATTTGGCGCA CGAATGGGAT 
TGCACTAAGT ACTATATCTG CGAACATGGA ACGTACGTCG AGCGAAGCTG TCCTTTGGGA 
CTTCAATGGA ATAAAACCTA CTGCGACTGG CCTACCAATG TTCAGTCCTC ATTGGGATCA 
AACCAGAGAA CTCAGGAGCC AGCGGTCCAT CGCCCCAATC CTACAAGTGT CATTACAGAG 
CCCCCAATCA TCAATAACTC ATACAAAGTA GTCTGTTACT TTACGAGCTG GGCATGGTAC 
CGTTCGTCAC AGGGCAAGTT TGTGCCTGAA GATATAGACG CCAACTTGTG CACCCACCTC 
ATTTACGGCT TCGCGGTGCT TGATAGCAAG TCGTTAACCA TTAAGACGCA TGACTCCTGG 
ACCGATATAG ACAACCGTTT CTATGAGCGA GTTGTAGAAT ACAAGCAAAG AGGCCTTCGA 
GTCATGTTAG CCATTGGTGG ATGGAATGAC TCGTTGGGCA GCAAGTACGC ACGTCTTGTT 
CTTAATTCAC AGTCCAGGAG GCGCTTTGTC GCAAGTGTGA TAAGTTTTCT AGAACAACAC 
GGTTTTGAGG GTCTGGACTT AGCCTGGGAG TTTCCTGTAT GCTGGCAAGT TAACTGCAAC 
AGAGGTAATC CCACGGAAAA GGATGGATTT GTTGCTCTTG TCAAAGAACT GTCGGAAGCC 
TTTAAGGAAA ACGGTCTTAT TCTTTCGGCA GCAGTCTCGC CCAGTAAAAT GGTTATTGAT 
GCCGGGTACA ATGTGTTTGA GCTCTCTCCG TACTTTGACT GGGTGGCCGT CATGACGTAC 
GACTTTCACG GTCACTGGGA TATGCGCACT GGCCAAATAG CCCCACTCTT TCACAGAGGG 
GGTGACGAAA ACCTATACCT TAACGGAAAC TTCAGCATCC ATTACTGGTT GGAAAGGGGT 
ATTCCAAACG ACAAGCTGGT TATGGGAATG CCAATGTACG GACAGACGTT TACGCTAGCT 
GACCAAAATC GCCGGTCACT AAATGATAAA ACAGTGGGTC CGGGGAAGGC AGGAACCTTT 
ACTAGAGCGG ATGGATTCTT GGCGTACTAT GAAATTTGCG AAAAAGTCGT CAACGATGAC 
TGGAAAGTGG TGAGGGATGA AGAAGGTATA TTCGGATCCT ATGCATATAG TGGCAACCAG 
TGGATATCAT ACGATGATGT CACAACTATT CGAAGAAAAT CTCAGTTTAT TAAGAGCTTG 
CAGCTAGGTG GCGGCATGAT TTGGGCCCTT GATTTAGATG ACTTTCGTGG ACTTTGCGGT 
TGCGGCAAGC ACCCATTACT CAGGACTCTT AGTCAGGAGC TCCTAGGCAT TCCTGGACAA 
AAGGCTAAGG ACTGCATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036422.1
CDS	137..6997
Translation	MHPYVPSVLVVVVLAISVKAHIRVDKLQIHSESNYKPAFIRSAVESIPLDIDAILLLRNSTKPEFGDAFLPLRSAVESIPKLYQSNTSRFRKNPDVRSFVRDSVESLPNDEDGLEHIEIFTKYYNSVSDVAGVDLKSKKLTHVNLDPGTISDKYAAFIARGNSEDYYPKSYRAGSPHQELLKMMNIEHTDKLDRYSDLQHQQSYGALGLANRNDLKMTKKVLCYMSNWAFYRSGEAHFVPEQIDPNLCSAIIYSFASLDPDHLTIREFDSWVDLDNQYYRRVTSLGVPVLIALGGWTDSSGSKYSRLVSDNLKRRVFISSVSSFLLRHGFSGLHLDWNYPKCWQSDCSRGPVTDRPNLTKLLRELRTEFQSVDPKFQLGVAISGYKEIIKEAYDFPALSDIVDYMTVMTYDYHGAWEQKTGHVSPLYGLSSDTYPQYNTNYTMQLLLKMGARREKLVLSIPFYGQSFTLATAHQILAGPGVAASGPGDAGELTKQPGMLAYYEICQRLTKFNWISDRNLNVIFGPFAMLNDQWVGYEDPTSAQAKARYAANNNFAGVAAWTIDLDDFRNLCCNESYPLLRAINRALGRLDSEPPTQPNCKRPTLLATPVPPQMTTISSDGSGGLGQNHDHTTSLPSGQISSSPVSTTITSTFPWWSSTTKRPREPTKTTAQPTHTTILIPAGINPVVQPSNCKSGEFFADSNNCNAYYHCFFAGELQQQFCPSGLHWNNEAKGCDWPSSAQCSLKLDQHLSTSYPNPIQTSKKPETTLKPNKKPSEISTHHQVNSTSSRPQYMRPTILECTEGDYYPHRNCRKYYICVNKALVPSECGGDLHWDGIKKLCDWPENVQCVTSKKYLKIIKSSSANEEDPCKGEKRVPYPGNCSKYLFCLWNRLQASDCPPGLHYNERIGNCDWPAAAKCNPKGSESSEEAELNAMPKPPTPQTPSSHLRPTYPTEKPVPKPRDSHYKVICYFTNWAWYRKGIGRFTPDDINTELCTHVIYGFAVLDYSELVLRTHDSWADVENNFYTRVTSLKSKGIKVSLALGGWNDSQGDKYSRLVRSPMARSRFVRHALEFIEKYGFEGLDLDWEYPVCWQTECNKGSTEEKDGFTAWVQELSEAFRPRGLMLSTAVSPSRKIIDAGYDIPQLSRYFDWIAVMTYDFHGHWDKKTGHVAPLYHHPDDDFEYFNVNYSINYWMEKGAPSQKLVMGIPLYGQSFTLENTNSSGLNAKAPAPGEAGEFTRAAGFLAYYEICERVNRQGWQVVHDEFGRMGPYAYKGTQWVSYDSPDMVRKKSLLVRSLKLGGGMVWALDLDDFKNRCGNGVHPLLTEIHNVLKDPPSLMEIPGPIETTPTEYPGMEEEIHESNGEGPEVQPIEAVMQTCENEGEEHEGILDPNHVLEEENIEATEMATEFKIICYFTNWAWYRQGGGKFLPEDIDSDLCTHIIYGFAVLSRDNLTIQPHDSWADLDNKFYERIVAYRKKGAKVTVAIGGWNDSAGDKYSRLVRNPEARSRFIRNVLDFIEEYNFDGLDLDWEYPVCWQVDCKKGTAEEKIGFSALVRELFYAFQPRGLILSAAVSPNKKVIDAGYEVAELSHYFSWISVMAYDYHGQWDKKTGHVAPMYSHPEGTANFNANFSMNYWISMGADRRKLVMGIPLYGQSFSLAETTKHQLNAPTYGGGEAGEATRARGFLAYYEICLKIRHHRWNVVRDTKGRIGPFAYHGDQWVSFDDVPMIRHKSEYIKAMGLGGAMIWALDLDDFKNVCECESYPLLKAINRVLRGFGGPQPKCLLENPKSTMKPNIKPPFRPTINAPSGPNLDSPTQNVSLNVLSIKCHSKNYLAHEWDCTKYYICEHGTYVERSCPLGLQWNKTYCDWPTNVQSSLGSNQRTQEPAVHRPNPTSVITEPPIINNSYKVVCYFTSWAWYRSSQGKFVPEDIDANLCTHLIYGFAVLDSKSLTIKTHDSWTDIDNRFYERVVEYKQRGLRVMLAIGGWNDSLGSKYARLVLNSQSRRRFVASVISFLEQHGFEGLDLAWEFPVCWQVNCNRGNPTEKDGFVALVKELSEAFKENGLILSAAVSPSKMVIDAGYNVFELSPYFDWVAVMTYDFHGHWDMRTGQIAPLFHRGGDENLYLNGNFSIHYWLERGIPNDKLVMGMPMYGQTFTLADQNRRSLNDKTVGPGKAGTFTRADGFLAYYEICEKVVNDDWKVVRDEEGIFGSYAYSGNQWISYDDVTTIRRKSQFIKSLQLGGGMIWALDLDDFRGLCGCGKHPLLRTLSQELLGIPGQKAKDCI

Target ORF information:

RefSeq Version	NM_001042957.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chitinase 10, transcript variant A (Cht10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042957.2

ORF Insert Sequence:

ATGCATCCGT ATGTCCCAAG TGTGCTTGTG GTGGTAGTCT TGGCTATATC TGTAAAAGCC 
CACATTCGCG TTGATAAACT GCAGATCCAT TCGGAATCCA ACTATAAACC TGCATTCATA 
CGTTCTGCGG TTGAGAGCAT TCCTTTGGAT ATTGATGCCA TACTCCTACT CCGTAACAGC 
ACAAAACCTG AGTTTGGGGA CGCTTTCCTA CCGCTGAGGA GTGCAGTGGA GAGTATTCCC 
AAGCTTTATC AGAGCAACAC GTCTAGGTTC AGAAAGAACC CTGATGTACG TTCTTTTGTG 
CGTGATTCTG TAGAGTCATT GCCAAATGAT GAAGACGGAC TGGAACACAT AGAAATTTTC 
ACTAAATATT ATAACAGTGT TAGCGATGTT GCAGGAGTTG ATCTAAAAAG CAAAAAGTTA 
ACACATGTGA ATTTAGATCC TGGGACCATT TCCGATAAGT ACGCAGCTTT TATTGCCCGC 
GGAAACTCAG AAGACTATTA TCCGAAGTCA TACCGAGCCG GCTCGCCGCA TCAGGAGTTG 
CTTAAAATGA TGAACATCGA ACACACCGAT AAACTTGATC GGTACTCTGA CCTGCAACAC 
CAGCAGTCCT ACGGAGCTTT GGGGTTGGCA AACAGGAATG ATCTCAAAAT GACTAAAAAA 
GTTCTTTGTT ACATGTCGAA TTGGGCATTC TACCGTAGTG GTGAGGCTCA TTTTGTGCCA 
GAGCAGATTG ATCCTAATCT ATGCTCTGCT ATTATCTATT CTTTTGCCTC CCTGGATCCT 
GATCACCTTA CAATTCGAGA ATTCGATTCA TGGGTGGACT TGGATAACCA ATACTACCGT 
CGAGTGACAT CGCTGGGTGT GCCTGTACTA ATTGCATTGG GTGGATGGAC GGACTCTAGC 
GGTTCCAAAT ACTCCCGATT AGTCAGTGAC AATCTTAAGC GACGTGTTTT TATATCCAGT 
GTGAGCAGCT TTTTACTACG TCATGGATTC AGCGGGCTCC ACCTAGACTG GAACTACCCG 
AAGTGCTGGC AGAGCGATTG CTCAAGGGGA CCTGTGACTG ACAGGCCAAA CCTGACTAAG 
CTTCTTCGTG AACTACGCAC CGAGTTTCAA AGTGTTGATC CGAAATTTCA GTTGGGAGTG 
GCCATCTCTG GCTACAAGGA GATTATAAAG GAGGCTTATG ATTTTCCCGC TTTGTCAGAC 
ATTGTAGACT ATATGACAGT AATGACATAC GATTACCACG GAGCTTGGGA ACAGAAAACA 
GGTCATGTGA GTCCACTTTA CGGCTTATCA TCGGACACTT ATCCTCAGTA CAATACAAAC 
TATACCATGC AGTTACTCCT AAAAATGGGA GCGCGAAGAG AAAAACTTGT ATTGAGCATT 
CCGTTTTACG GCCAGAGTTT TACATTGGCA ACCGCCCACC AAATTCTTGC AGGCCCAGGA 
GTGGCAGCCT CTGGACCTGG AGATGCCGGT GAATTGACAA AGCAACCTGG AATGCTAGCT 
TACTACGAGA TTTGTCAGCG CCTAACAAAG TTTAATTGGA TAAGTGATCG GAATTTAAAC 
GTGATATTCG GTCCATTCGC TATGCTAAAC GACCAGTGGG TAGGATATGA AGACCCCACC 
AGTGCCCAAG CCAAAGCTCG ATATGCGGCT AACAACAACT TTGCCGGAGT GGCAGCCTGG 
ACCATTGACC TTGATGACTT CCGAAACCTA TGTTGCAATG AATCTTATCC TTTACTTAGG 
GCCATTAATA GAGCATTGGG TCGCTTGGAC TCGGAGCCGC CAACTCAACC GAACTGTAAG 
CGTCCTACAT TATTGGCGAC ACCAGTGCCG CCTCAAATGA CAACAATTAG TAGCGATGGT 
TCTGGGGGAT TGGGACAAAA TCACGATCAT ACAACTTCCT TGCCAAGCGG GCAGATCAGC 
AGTAGTCCTG TAAGTACCAC TATCACGAGC ACCTTCCCCT GGTGGAGTTC AACTACAAAA 
CGTCCGCGCG AGCCGACTAA GACTACAGCA CAACCAACTC ACACCACAAT CCTCATTCCA 
GCAGGGATCA ACCCAGTGGT ACAGCCCTCG AATTGCAAAA GCGGCGAGTT CTTCGCTGAC 
TCAAATAATT GTAACGCTTA CTATCACTGT TTTTTTGCGG GAGAGTTACA GCAACAGTTT 
TGTCCAAGCG GTCTTCACTG GAACAACGAA GCTAAAGGAT GTGACTGGCC GTCGTCCGCC 
CAATGCTCAC TAAAACTCGA TCAACACCTA AGTACTTCTT ATCCAAACCC GATTCAAACG 
TCTAAAAAGC CGGAGACGAC TTTGAAACCT AATAAGAAAC CGTCAGAGAT TTCCACACAT 
CATCAGGTAA ATTCTACCTC AAGCCGACCA CAATATATGC GTCCAACTAT ATTGGAGTGC 
ACTGAAGGTG ATTACTACCC ACACAGAAAC TGCCGGAAAT ATTACATCTG CGTTAATAAG 
GCACTGGTAC CCAGTGAATG TGGCGGAGAC TTGCATTGGG ACGGTATCAA AAAACTTTGT 
GACTGGCCTG AGAACGTACA ATGCGTAACT AGCAAAAAAT ATTTAAAAAT AATCAAATCG 
AGTAGCGCCA ACGAAGAAGA CCCGTGCAAA GGCGAAAAGA GAGTACCCTA TCCAGGAAAC 
TGTTCGAAAT ATCTCTTCTG CCTATGGAAC CGCCTTCAGG CTAGTGACTG TCCACCAGGG 
CTGCACTATA ATGAAAGAAT TGGGAACTGC GATTGGCCAG CAGCTGCAAA ATGCAATCCA 
AAAGGTAGTG AGAGCAGCGA AGAGGCAGAG CTGAACGCTA TGCCGAAGCC TCCGACTCCA 
CAAACCCCCA GTAGTCATTT GCGTCCAACG TACCCCACAG AAAAACCAGT TCCCAAACCT 
CGTGATAGTC ACTACAAAGT TATTTGCTAC TTTACAAACT GGGCCTGGTA CCGCAAAGGA 
ATAGGTCGCT TTACTCCCGA CGACATAAAC ACGGAGCTGT GCACTCACGT AATTTATGGG 
TTTGCAGTGT TAGACTATTC CGAACTTGTA CTACGAACAC ATGATTCCTG GGCTGACGTT 
GAAAACAACT TTTACACAAG GGTCACCAGC CTCAAAAGCA AGGGCATCAA GGTTAGCTTA 
GCACTTGGTG GATGGAACGA TTCGCAAGGC GATAAGTACA GCCGCCTAGT TCGAAGTCCA 
ATGGCCCGGT CCCGTTTTGT GCGCCACGCC CTCGAGTTTA TTGAAAAATA CGGCTTTGAG 
GGCCTTGATT TAGATTGGGA GTACCCAGTC TGCTGGCAAA CGGAGTGCAA CAAAGGGTCT 
ACCGAGGAAA AGGACGGTTT CACTGCCTGG GTACAGGAGC TTTCTGAAGC GTTTCGCCCT 
CGTGGACTTA TGTTGTCCAC TGCTGTCTCG CCTAGCAGGA AAATCATTGA TGCCGGATAC 
GACATCCCTC AGCTCTCTCG CTATTTTGAT TGGATCGCTG TCATGACATA TGATTTTCAT 
GGACATTGGG ATAAAAAAAC AGGACATGTG GCACCGCTGT ATCATCATCC CGATGATGAC 
TTTGAATATT TTAACGTGAA CTATTCTATA AACTATTGGA TGGAAAAAGG AGCACCATCA 
CAAAAATTAG TAATGGGTAT TCCGCTATAC GGCCAGTCGT TCACTTTGGA AAATACCAAC 
AGCAGTGGCT TAAATGCCAA AGCCCCTGCA CCAGGTGAAG CTGGAGAGTT CACCAGAGCA 
GCTGGATTTC TTGCTTACTA TGAGATTTGT GAACGAGTAA ATCGCCAAGG CTGGCAGGTA 
GTGCATGATG AATTTGGGCG CATGGGACCT TATGCTTACA AGGGAACTCA GTGGGTGTCC 
TACGATAGCC CCGATATGGT CAGGAAAAAA TCACTACTGG TGAGGTCATT GAAACTGGGC 
GGTGGAATGG TTTGGGCTCT TGATCTGGAT GACTTTAAAA ATCGGTGTGG AAATGGCGTG 
CATCCTTTGC TCACTGAAAT CCATAACGTG CTTAAGGATC CACCTAGTTT AATGGAAATA 
CCTGGTCCTA TCGAAACGAC TCCTACAGAG TACCCAGGCA TGGAAGAAGA GATACATGAG 
TCTAATGGGG AGGGTCCCGA GGTGCAGCCG ATTGAAGCGG TGATGCAGAC CTGCGAAAAT 
GAAGGTGAAG AACATGAAGG TATTTTAGAC CCAAATCACG TCCTTGAGGA GGAAAACATA 
GAAGCTACTG AAATGGCCAC GGAATTTAAA ATCATTTGCT ACTTTACCAA CTGGGCGTGG 
TACCGTCAAG GTGGTGGTAA GTTCCTGCCG GAAGATATTG ATTCGGATCT GTGTACCCAC 
ATAATTTATG GCTTTGCCGT GTTGAGTCGG GACAACCTTA CGATTCAGCC TCATGACTCT 
TGGGCGGACT TAGACAACAA GTTTTATGAG CGGATTGTGG CCTACCGCAA GAAAGGCGCT 
AAAGTAACTG TGGCAATTGG CGGTTGGAAT GATTCTGCTG GCGATAAGTA CTCCCGACTT 
GTAAGGAATC CAGAGGCAAG ATCTCGGTTT ATCCGTAATG TCTTGGATTT TATAGAGGAA 
TATAATTTCG ACGGCTTGGA CCTGGATTGG GAGTACCCCG TTTGCTGGCA GGTGGATTGC 
AAGAAAGGGA CGGCAGAAGA GAAGATTGGA TTCTCAGCTT TGGTGCGGGA GCTTTTTTAT 
GCCTTCCAGC CCAGGGGATT AATTCTGTCA GCTGCCGTGT CACCTAACAA GAAGGTAATC 
GATGCTGGAT ACGAAGTTGC GGAGCTATCC CATTACTTTT CTTGGATATC TGTGATGGCC 
TATGACTACC ACGGTCAGTG GGATAAGAAG ACTGGCCATG TAGCTCCAAT GTACAGCCAC 
CCAGAAGGAA CAGCAAATTT TAACGCCAAT TTTTCCATGA ACTACTGGAT CTCGATGGGA 
GCTGACCGGC GAAAGTTAGT AATGGGAATA CCTCTGTACG GCCAGTCATT CTCTCTGGCT 
GAGACTACTA AGCACCAACT AAATGCTCCC ACGTATGGTG GCGGTGAAGC TGGAGAGGCT 
ACAAGAGCCC GAGGATTTTT GGCATACTAT GAAATATGCT TGAAAATCCG ACATCATAGG 
TGGAACGTTG TAAGGGATAC CAAGGGTCGA ATAGGACCCT TCGCATATCA TGGGGATCAG 
TGGGTTTCGT TCGATGATGT ACCCATGATT CGGCATAAAA GCGAGTACAT CAAGGCGATG 
GGTCTTGGTG GAGCTATGAT ATGGGCTCTA GACTTGGATG ACTTCAAAAA TGTGTGCGAA 
TGCGAGTCAT ATCCCTTGCT TAAAGCTATT AATCGTGTAC TCAGAGGTTT CGGGGGTCCC 
CAACCGAAGT GCTTACTTGA AAACCCCAAA AGCACAATGA AGCCAAACAT CAAACCGCCT 
TTCAGGCCAA CAATAAATGC CCCATCTGGA CCTAATCTTG ATTCTCCTAC CCAAAATGTA 
TCCTTAAATG TCCTGTCCAT CAAATGCCAC AGCAAAAACT ATTTGGCGCA CGAATGGGAT 
TGCACTAAGT ACTATATCTG CGAACATGGA ACGTACGTCG AGCGAAGCTG TCCTTTGGGA 
CTTCAATGGA ATAAAACCTA CTGCGACTGG CCTACCAATG TTCAGTCCTC ATTGGGATCA 
AACCAGAGAA CTCAGGAGCC AGCGGTCCAT CGCCCCAATC CTACAAGTGT CATTACAGAG 
CCCCCAATCA TCAATAACTC ATACAAAGTA GTCTGTTACT TTACGAGCTG GGCATGGTAC 
CGTTCGTCAC AGGGCAAGTT TGTGCCTGAA GATATAGACG CCAACTTGTG CACCCACCTC 
ATTTACGGCT TCGCGGTGCT TGATAGCAAG TCGTTAACCA TTAAGACGCA TGACTCCTGG 
ACCGATATAG ACAACCGTTT CTATGAGCGA GTTGTAGAAT ACAAGCAAAG AGGCCTTCGA 
GTCATGTTAG CCATTGGTGG ATGGAATGAC TCGTTGGGCA GCAAGTACGC ACGTCTTGTT 
CTTAATTCAC AGTCCAGGAG GCGCTTTGTC GCAAGTGTGA TAAGTTTTCT AGAACAACAC 
GGTTTTGAGG GTCTGGACTT AGCCTGGGAG TTTCCTGTAT GCTGGCAAGT TAACTGCAAC 
AGAGGTAATC CCACGGAAAA GGATGGATTT GTTGCTCTTG TCAAAGAACT GTCGGAAGCC 
TTTAAGGAAA ACGGTCTTAT TCTTTCGGCA GCAGTCTCGC CCAGTAAAAT GGTTATTGAT 
GCCGGGTACA ATGTGTTTGA GCTCTCTCCG TACTTTGACT GGGTGGCCGT CATGACGTAC 
GACTTTCACG GTCACTGGGA TATGCGCACT GGCCAAATAG CCCCACTCTT TCACAGAGGG 
GGTGACGAAA ACCTATACCT TAACGGAAAC TTCAGCATCC ATTACTGGTT GGAAAGGGGT 
ATTCCAAACG ACAAGCTGGT TATGGGAATG CCAATGTACG GACAGACGTT TACGCTAGCT 
GACCAAAATC GCCGGTCACT AAATGATAAA ACAGTGGGTC CGGGGAAGGC AGGAACCTTT 
ACTAGAGCGG ATGGATTCTT GGCGTACTAT GAAATTTGCG AAAAAGTCGT CAACGATGAC 
TGGAAAGTGG TGAGGGATGA AGAAGGTATA TTCGGATCCT ATGCATATAG TGGCAACCAG 
TGGATATCAT ACGATGATGT CACAACTATT CGAAGAAAAT CTCAGTTTAT TAAGAGCTTG 
CAGCTAGGTG GCGGCATGAT TTGGGCCCTT GATTTAGATG ACTTTCGTGG ACTTTGCGGT 
TGCGGCAAGC ACCCATTACT CAGGACTCTT AGTCAGGAGC TCCTAGGCAT TCCTGGACAA 
AAGGCTAAGG ACTGCATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cht10 ( NM_001273768.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260697.1 Drosophila melanogaster chitinase 10, transcript variant B (Cht10), mRNA.

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CloneID	OFa15554
Clone ID Related Accession (Same CDS sequence)	NM_001273769.1 , NM_001042957.2 , NM_001273768.1
Accession Version	NM_001273768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chitinase 10, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCCGT ATGTCCCAAG TGTGCTTGTG GTGGTAGTCT TGGCTATATC TGTAAAAGCC 
CACATTCGCG TTGATAAACT GCAGATCCAT TCGGAATCCA ACTATAAACC TGCATTCATA 
CGTTCTGCGG TTGAGAGCAT TCCTTTGGAT ATTGATGCCA TACTCCTACT CCGTAACAGC 
ACAAAACCTG AGTTTGGGGA CGCTTTCCTA CCGCTGAGGA GTGCAGTGGA GAGTATTCCC 
AAGCTTTATC AGAGCAACAC GTCTAGGTTC AGAAAGAACC CTGATGTACG TTCTTTTGTG 
CGTGATTCTG TAGAGTCATT GCCAAATGAT GAAGACGGAC TGGAACACAT AGAAATTTTC 
ACTAAATATT ATAACAGTGT TAGCGATGTT GCAGGAGTTG ATCTAAAAAG CAAAAAGTTA 
ACACATGTGA ATTTAGATCC TGGGACCATT TCCGATAAGT ACGCAGCTTT TATTGCCCGC 
GGAAACTCAG AAGACTATTA TCCGAAGTCA TACCGAGCCG GCTCGCCGCA TCAGGAGTTG 
CTTAAAATGA TGAACATCGA ACACACCGAT AAACTTGATC GGTACTCTGA CCTGCAACAC 
CAGCAGTCCT ACGGAGCTTT GGGGTTGGCA AACAGGAATG ATCTCAAAAT GACTAAAAAA 
GTTCTTTGTT ACATGTCGAA TTGGGCATTC TACCGTAGTG GTGAGGCTCA TTTTGTGCCA 
GAGCAGATTG ATCCTAATCT ATGCTCTGCT ATTATCTATT CTTTTGCCTC CCTGGATCCT 
GATCACCTTA CAATTCGAGA ATTCGATTCA TGGGTGGACT TGGATAACCA ATACTACCGT 
CGAGTGACAT CGCTGGGTGT GCCTGTACTA ATTGCATTGG GTGGATGGAC GGACTCTAGC 
GGTTCCAAAT ACTCCCGATT AGTCAGTGAC AATCTTAAGC GACGTGTTTT TATATCCAGT 
GTGAGCAGCT TTTTACTACG TCATGGATTC AGCGGGCTCC ACCTAGACTG GAACTACCCG 
AAGTGCTGGC AGAGCGATTG CTCAAGGGGA CCTGTGACTG ACAGGCCAAA CCTGACTAAG 
CTTCTTCGTG AACTACGCAC CGAGTTTCAA AGTGTTGATC CGAAATTTCA GTTGGGAGTG 
GCCATCTCTG GCTACAAGGA GATTATAAAG GAGGCTTATG ATTTTCCCGC TTTGTCAGAC 
ATTGTAGACT ATATGACAGT AATGACATAC GATTACCACG GAGCTTGGGA ACAGAAAACA 
GGTCATGTGA GTCCACTTTA CGGCTTATCA TCGGACACTT ATCCTCAGTA CAATACAAAC 
TATACCATGC AGTTACTCCT AAAAATGGGA GCGCGAAGAG AAAAACTTGT ATTGAGCATT 
CCGTTTTACG GCCAGAGTTT TACATTGGCA ACCGCCCACC AAATTCTTGC AGGCCCAGGA 
GTGGCAGCCT CTGGACCTGG AGATGCCGGT GAATTGACAA AGCAACCTGG AATGCTAGCT 
TACTACGAGA TTTGTCAGCG CCTAACAAAG TTTAATTGGA TAAGTGATCG GAATTTAAAC 
GTGATATTCG GTCCATTCGC TATGCTAAAC GACCAGTGGG TAGGATATGA AGACCCCACC 
AGTGCCCAAG CCAAAGCTCG ATATGCGGCT AACAACAACT TTGCCGGAGT GGCAGCCTGG 
ACCATTGACC TTGATGACTT CCGAAACCTA TGTTGCAATG AATCTTATCC TTTACTTAGG 
GCCATTAATA GAGCATTGGG TCGCTTGGAC TCGGAGCCGC CAACTCAACC GAACTGTAAG 
CGTCCTACAT TATTGGCGAC ACCAGTGCCG CCTCAAATGA CAACAATTAG TAGCGATGGT 
TCTGGGGGAT TGGGACAAAA TCACGATCAT ACAACTTCCT TGCCAAGCGG GCAGATCAGC 
AGTAGTCCTG TAAGTACCAC TATCACGAGC ACCTTCCCCT GGTGGAGTTC AACTACAAAA 
CGTCCGCGCG AGCCGACTAA GACTACAGCA CAACCAACTC ACACCACAAT CCTCATTCCA 
GCAGGGATCA ACCCAGTGGT ACAGCCCTCG AATTGCAAAA GCGGCGAGTT CTTCGCTGAC 
TCAAATAATT GTAACGCTTA CTATCACTGT TTTTTTGCGG GAGAGTTACA GCAACAGTTT 
TGTCCAAGCG GTCTTCACTG GAACAACGAA GCTAAAGGAT GTGACTGGCC GTCGTCCGCC 
CAATGCTCAC TAAAACTCGA TCAACACCTA AGTACTTCTT ATCCAAACCC GATTCAAACG 
TCTAAAAAGC CGGAGACGAC TTTGAAACCT AATAAGAAAC CGTCAGAGAT TTCCACACAT 
CATCAGGTAA ATTCTACCTC AAGCCGACCA CAATATATGC GTCCAACTAT ATTGGAGTGC 
ACTGAAGGTG ATTACTACCC ACACAGAAAC TGCCGGAAAT ATTACATCTG CGTTAATAAG 
GCACTGGTAC CCAGTGAATG TGGCGGAGAC TTGCATTGGG ACGGTATCAA AAAACTTTGT 
GACTGGCCTG AGAACGTACA ATGCGTAACT AGCAAAAAAT ATTTAAAAAT AATCAAATCG 
AGTAGCGCCA ACGAAGAAGA CCCGTGCAAA GGCGAAAAGA GAGTACCCTA TCCAGGAAAC 
TGTTCGAAAT ATCTCTTCTG CCTATGGAAC CGCCTTCAGG CTAGTGACTG TCCACCAGGG 
CTGCACTATA ATGAAAGAAT TGGGAACTGC GATTGGCCAG CAGCTGCAAA ATGCAATCCA 
AAAGGTAGTG AGAGCAGCGA AGAGGCAGAG CTGAACGCTA TGCCGAAGCC TCCGACTCCA 
CAAACCCCCA GTAGTCATTT GCGTCCAACG TACCCCACAG AAAAACCAGT TCCCAAACCT 
CGTGATAGTC ACTACAAAGT TATTTGCTAC TTTACAAACT GGGCCTGGTA CCGCAAAGGA 
ATAGGTCGCT TTACTCCCGA CGACATAAAC ACGGAGCTGT GCACTCACGT AATTTATGGG 
TTTGCAGTGT TAGACTATTC CGAACTTGTA CTACGAACAC ATGATTCCTG GGCTGACGTT 
GAAAACAACT TTTACACAAG GGTCACCAGC CTCAAAAGCA AGGGCATCAA GGTTAGCTTA 
GCACTTGGTG GATGGAACGA TTCGCAAGGC GATAAGTACA GCCGCCTAGT TCGAAGTCCA 
ATGGCCCGGT CCCGTTTTGT GCGCCACGCC CTCGAGTTTA TTGAAAAATA CGGCTTTGAG 
GGCCTTGATT TAGATTGGGA GTACCCAGTC TGCTGGCAAA CGGAGTGCAA CAAAGGGTCT 
ACCGAGGAAA AGGACGGTTT CACTGCCTGG GTACAGGAGC TTTCTGAAGC GTTTCGCCCT 
CGTGGACTTA TGTTGTCCAC TGCTGTCTCG CCTAGCAGGA AAATCATTGA TGCCGGATAC 
GACATCCCTC AGCTCTCTCG CTATTTTGAT TGGATCGCTG TCATGACATA TGATTTTCAT 
GGACATTGGG ATAAAAAAAC AGGACATGTG GCACCGCTGT ATCATCATCC CGATGATGAC 
TTTGAATATT TTAACGTGAA CTATTCTATA AACTATTGGA TGGAAAAAGG AGCACCATCA 
CAAAAATTAG TAATGGGTAT TCCGCTATAC GGCCAGTCGT TCACTTTGGA AAATACCAAC 
AGCAGTGGCT TAAATGCCAA AGCCCCTGCA CCAGGTGAAG CTGGAGAGTT CACCAGAGCA 
GCTGGATTTC TTGCTTACTA TGAGATTTGT GAACGAGTAA ATCGCCAAGG CTGGCAGGTA 
GTGCATGATG AATTTGGGCG CATGGGACCT TATGCTTACA AGGGAACTCA GTGGGTGTCC 
TACGATAGCC CCGATATGGT CAGGAAAAAA TCACTACTGG TGAGGTCATT GAAACTGGGC 
GGTGGAATGG TTTGGGCTCT TGATCTGGAT GACTTTAAAA ATCGGTGTGG AAATGGCGTG 
CATCCTTTGC TCACTGAAAT CCATAACGTG CTTAAGGATC CACCTAGTTT AATGGAAATA 
CCTGGTCCTA TCGAAACGAC TCCTACAGAG TACCCAGGCA TGGAAGAAGA GATACATGAG 
TCTAATGGGG AGGGTCCCGA GGTGCAGCCG ATTGAAGCGG TGATGCAGAC CTGCGAAAAT 
GAAGGTGAAG AACATGAAGG TATTTTAGAC CCAAATCACG TCCTTGAGGA GGAAAACATA 
GAAGCTACTG AAATGGCCAC GGAATTTAAA ATCATTTGCT ACTTTACCAA CTGGGCGTGG 
TACCGTCAAG GTGGTGGTAA GTTCCTGCCG GAAGATATTG ATTCGGATCT GTGTACCCAC 
ATAATTTATG GCTTTGCCGT GTTGAGTCGG GACAACCTTA CGATTCAGCC TCATGACTCT 
TGGGCGGACT TAGACAACAA GTTTTATGAG CGGATTGTGG CCTACCGCAA GAAAGGCGCT 
AAAGTAACTG TGGCAATTGG CGGTTGGAAT GATTCTGCTG GCGATAAGTA CTCCCGACTT 
GTAAGGAATC CAGAGGCAAG ATCTCGGTTT ATCCGTAATG TCTTGGATTT TATAGAGGAA 
TATAATTTCG ACGGCTTGGA CCTGGATTGG GAGTACCCCG TTTGCTGGCA GGTGGATTGC 
AAGAAAGGGA CGGCAGAAGA GAAGATTGGA TTCTCAGCTT TGGTGCGGGA GCTTTTTTAT 
GCCTTCCAGC CCAGGGGATT AATTCTGTCA GCTGCCGTGT CACCTAACAA GAAGGTAATC 
GATGCTGGAT ACGAAGTTGC GGAGCTATCC CATTACTTTT CTTGGATATC TGTGATGGCC 
TATGACTACC ACGGTCAGTG GGATAAGAAG ACTGGCCATG TAGCTCCAAT GTACAGCCAC 
CCAGAAGGAA CAGCAAATTT TAACGCCAAT TTTTCCATGA ACTACTGGAT CTCGATGGGA 
GCTGACCGGC GAAAGTTAGT AATGGGAATA CCTCTGTACG GCCAGTCATT CTCTCTGGCT 
GAGACTACTA AGCACCAACT AAATGCTCCC ACGTATGGTG GCGGTGAAGC TGGAGAGGCT 
ACAAGAGCCC GAGGATTTTT GGCATACTAT GAAATATGCT TGAAAATCCG ACATCATAGG 
TGGAACGTTG TAAGGGATAC CAAGGGTCGA ATAGGACCCT TCGCATATCA TGGGGATCAG 
TGGGTTTCGT TCGATGATGT ACCCATGATT CGGCATAAAA GCGAGTACAT CAAGGCGATG 
GGTCTTGGTG GAGCTATGAT ATGGGCTCTA GACTTGGATG ACTTCAAAAA TGTGTGCGAA 
TGCGAGTCAT ATCCCTTGCT TAAAGCTATT AATCGTGTAC TCAGAGGTTT CGGGGGTCCC 
CAACCGAAGT GCTTACTTGA AAACCCCAAA AGCACAATGA AGCCAAACAT CAAACCGCCT 
TTCAGGCCAA CAATAAATGC CCCATCTGGA CCTAATCTTG ATTCTCCTAC CCAAAATGTA 
TCCTTAAATG TCCTGTCCAT CAAATGCCAC AGCAAAAACT ATTTGGCGCA CGAATGGGAT 
TGCACTAAGT ACTATATCTG CGAACATGGA ACGTACGTCG AGCGAAGCTG TCCTTTGGGA 
CTTCAATGGA ATAAAACCTA CTGCGACTGG CCTACCAATG TTCAGTCCTC ATTGGGATCA 
AACCAGAGAA CTCAGGAGCC AGCGGTCCAT CGCCCCAATC CTACAAGTGT CATTACAGAG 
CCCCCAATCA TCAATAACTC ATACAAAGTA GTCTGTTACT TTACGAGCTG GGCATGGTAC 
CGTTCGTCAC AGGGCAAGTT TGTGCCTGAA GATATAGACG CCAACTTGTG CACCCACCTC 
ATTTACGGCT TCGCGGTGCT TGATAGCAAG TCGTTAACCA TTAAGACGCA TGACTCCTGG 
ACCGATATAG ACAACCGTTT CTATGAGCGA GTTGTAGAAT ACAAGCAAAG AGGCCTTCGA 
GTCATGTTAG CCATTGGTGG ATGGAATGAC TCGTTGGGCA GCAAGTACGC ACGTCTTGTT 
CTTAATTCAC AGTCCAGGAG GCGCTTTGTC GCAAGTGTGA TAAGTTTTCT AGAACAACAC 
GGTTTTGAGG GTCTGGACTT AGCCTGGGAG TTTCCTGTAT GCTGGCAAGT TAACTGCAAC 
AGAGGTAATC CCACGGAAAA GGATGGATTT GTTGCTCTTG TCAAAGAACT GTCGGAAGCC 
TTTAAGGAAA ACGGTCTTAT TCTTTCGGCA GCAGTCTCGC CCAGTAAAAT GGTTATTGAT 
GCCGGGTACA ATGTGTTTGA GCTCTCTCCG TACTTTGACT GGGTGGCCGT CATGACGTAC 
GACTTTCACG GTCACTGGGA TATGCGCACT GGCCAAATAG CCCCACTCTT TCACAGAGGG 
GGTGACGAAA ACCTATACCT TAACGGAAAC TTCAGCATCC ATTACTGGTT GGAAAGGGGT 
ATTCCAAACG ACAAGCTGGT TATGGGAATG CCAATGTACG GACAGACGTT TACGCTAGCT 
GACCAAAATC GCCGGTCACT AAATGATAAA ACAGTGGGTC CGGGGAAGGC AGGAACCTTT 
ACTAGAGCGG ATGGATTCTT GGCGTACTAT GAAATTTGCG AAAAAGTCGT CAACGATGAC 
TGGAAAGTGG TGAGGGATGA AGAAGGTATA TTCGGATCCT ATGCATATAG TGGCAACCAG 
TGGATATCAT ACGATGATGT CACAACTATT CGAAGAAAAT CTCAGTTTAT TAAGAGCTTG 
CAGCTAGGTG GCGGCATGAT TTGGGCCCTT GATTTAGATG ACTTTCGTGG ACTTTGCGGT 
TGCGGCAAGC ACCCATTACT CAGGACTCTT AGTCAGGAGC TCCTAGGCAT TCCTGGACAA 
AAGGCTAAGG ACTGCATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260697.1
CDS	221..7081
Translation	MHPYVPSVLVVVVLAISVKAHIRVDKLQIHSESNYKPAFIRSAVESIPLDIDAILLLRNSTKPEFGDAFLPLRSAVESIPKLYQSNTSRFRKNPDVRSFVRDSVESLPNDEDGLEHIEIFTKYYNSVSDVAGVDLKSKKLTHVNLDPGTISDKYAAFIARGNSEDYYPKSYRAGSPHQELLKMMNIEHTDKLDRYSDLQHQQSYGALGLANRNDLKMTKKVLCYMSNWAFYRSGEAHFVPEQIDPNLCSAIIYSFASLDPDHLTIREFDSWVDLDNQYYRRVTSLGVPVLIALGGWTDSSGSKYSRLVSDNLKRRVFISSVSSFLLRHGFSGLHLDWNYPKCWQSDCSRGPVTDRPNLTKLLRELRTEFQSVDPKFQLGVAISGYKEIIKEAYDFPALSDIVDYMTVMTYDYHGAWEQKTGHVSPLYGLSSDTYPQYNTNYTMQLLLKMGARREKLVLSIPFYGQSFTLATAHQILAGPGVAASGPGDAGELTKQPGMLAYYEICQRLTKFNWISDRNLNVIFGPFAMLNDQWVGYEDPTSAQAKARYAANNNFAGVAAWTIDLDDFRNLCCNESYPLLRAINRALGRLDSEPPTQPNCKRPTLLATPVPPQMTTISSDGSGGLGQNHDHTTSLPSGQISSSPVSTTITSTFPWWSSTTKRPREPTKTTAQPTHTTILIPAGINPVVQPSNCKSGEFFADSNNCNAYYHCFFAGELQQQFCPSGLHWNNEAKGCDWPSSAQCSLKLDQHLSTSYPNPIQTSKKPETTLKPNKKPSEISTHHQVNSTSSRPQYMRPTILECTEGDYYPHRNCRKYYICVNKALVPSECGGDLHWDGIKKLCDWPENVQCVTSKKYLKIIKSSSANEEDPCKGEKRVPYPGNCSKYLFCLWNRLQASDCPPGLHYNERIGNCDWPAAAKCNPKGSESSEEAELNAMPKPPTPQTPSSHLRPTYPTEKPVPKPRDSHYKVICYFTNWAWYRKGIGRFTPDDINTELCTHVIYGFAVLDYSELVLRTHDSWADVENNFYTRVTSLKSKGIKVSLALGGWNDSQGDKYSRLVRSPMARSRFVRHALEFIEKYGFEGLDLDWEYPVCWQTECNKGSTEEKDGFTAWVQELSEAFRPRGLMLSTAVSPSRKIIDAGYDIPQLSRYFDWIAVMTYDFHGHWDKKTGHVAPLYHHPDDDFEYFNVNYSINYWMEKGAPSQKLVMGIPLYGQSFTLENTNSSGLNAKAPAPGEAGEFTRAAGFLAYYEICERVNRQGWQVVHDEFGRMGPYAYKGTQWVSYDSPDMVRKKSLLVRSLKLGGGMVWALDLDDFKNRCGNGVHPLLTEIHNVLKDPPSLMEIPGPIETTPTEYPGMEEEIHESNGEGPEVQPIEAVMQTCENEGEEHEGILDPNHVLEEENIEATEMATEFKIICYFTNWAWYRQGGGKFLPEDIDSDLCTHIIYGFAVLSRDNLTIQPHDSWADLDNKFYERIVAYRKKGAKVTVAIGGWNDSAGDKYSRLVRNPEARSRFIRNVLDFIEEYNFDGLDLDWEYPVCWQVDCKKGTAEEKIGFSALVRELFYAFQPRGLILSAAVSPNKKVIDAGYEVAELSHYFSWISVMAYDYHGQWDKKTGHVAPMYSHPEGTANFNANFSMNYWISMGADRRKLVMGIPLYGQSFSLAETTKHQLNAPTYGGGEAGEATRARGFLAYYEICLKIRHHRWNVVRDTKGRIGPFAYHGDQWVSFDDVPMIRHKSEYIKAMGLGGAMIWALDLDDFKNVCECESYPLLKAINRVLRGFGGPQPKCLLENPKSTMKPNIKPPFRPTINAPSGPNLDSPTQNVSLNVLSIKCHSKNYLAHEWDCTKYYICEHGTYVERSCPLGLQWNKTYCDWPTNVQSSLGSNQRTQEPAVHRPNPTSVITEPPIINNSYKVVCYFTSWAWYRSSQGKFVPEDIDANLCTHLIYGFAVLDSKSLTIKTHDSWTDIDNRFYERVVEYKQRGLRVMLAIGGWNDSLGSKYARLVLNSQSRRRFVASVISFLEQHGFEGLDLAWEFPVCWQVNCNRGNPTEKDGFVALVKELSEAFKENGLILSAAVSPSKMVIDAGYNVFELSPYFDWVAVMTYDFHGHWDMRTGQIAPLFHRGGDENLYLNGNFSIHYWLERGIPNDKLVMGMPMYGQTFTLADQNRRSLNDKTVGPGKAGTFTRADGFLAYYEICEKVVNDDWKVVRDEEGIFGSYAYSGNQWISYDDVTTIRRKSQFIKSLQLGGGMIWALDLDDFRGLCGCGKHPLLRTLSQELLGIPGQKAKDCI

Target ORF information:

RefSeq Version	NM_001273768.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chitinase 10, transcript variant B (Cht10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273768.1

ORF Insert Sequence:

ATGCATCCGT ATGTCCCAAG TGTGCTTGTG GTGGTAGTCT TGGCTATATC TGTAAAAGCC 
CACATTCGCG TTGATAAACT GCAGATCCAT TCGGAATCCA ACTATAAACC TGCATTCATA 
CGTTCTGCGG TTGAGAGCAT TCCTTTGGAT ATTGATGCCA TACTCCTACT CCGTAACAGC 
ACAAAACCTG AGTTTGGGGA CGCTTTCCTA CCGCTGAGGA GTGCAGTGGA GAGTATTCCC 
AAGCTTTATC AGAGCAACAC GTCTAGGTTC AGAAAGAACC CTGATGTACG TTCTTTTGTG 
CGTGATTCTG TAGAGTCATT GCCAAATGAT GAAGACGGAC TGGAACACAT AGAAATTTTC 
ACTAAATATT ATAACAGTGT TAGCGATGTT GCAGGAGTTG ATCTAAAAAG CAAAAAGTTA 
ACACATGTGA ATTTAGATCC TGGGACCATT TCCGATAAGT ACGCAGCTTT TATTGCCCGC 
GGAAACTCAG AAGACTATTA TCCGAAGTCA TACCGAGCCG GCTCGCCGCA TCAGGAGTTG 
CTTAAAATGA TGAACATCGA ACACACCGAT AAACTTGATC GGTACTCTGA CCTGCAACAC 
CAGCAGTCCT ACGGAGCTTT GGGGTTGGCA AACAGGAATG ATCTCAAAAT GACTAAAAAA 
GTTCTTTGTT ACATGTCGAA TTGGGCATTC TACCGTAGTG GTGAGGCTCA TTTTGTGCCA 
GAGCAGATTG ATCCTAATCT ATGCTCTGCT ATTATCTATT CTTTTGCCTC CCTGGATCCT 
GATCACCTTA CAATTCGAGA ATTCGATTCA TGGGTGGACT TGGATAACCA ATACTACCGT 
CGAGTGACAT CGCTGGGTGT GCCTGTACTA ATTGCATTGG GTGGATGGAC GGACTCTAGC 
GGTTCCAAAT ACTCCCGATT AGTCAGTGAC AATCTTAAGC GACGTGTTTT TATATCCAGT 
GTGAGCAGCT TTTTACTACG TCATGGATTC AGCGGGCTCC ACCTAGACTG GAACTACCCG 
AAGTGCTGGC AGAGCGATTG CTCAAGGGGA CCTGTGACTG ACAGGCCAAA CCTGACTAAG 
CTTCTTCGTG AACTACGCAC CGAGTTTCAA AGTGTTGATC CGAAATTTCA GTTGGGAGTG 
GCCATCTCTG GCTACAAGGA GATTATAAAG GAGGCTTATG ATTTTCCCGC TTTGTCAGAC 
ATTGTAGACT ATATGACAGT AATGACATAC GATTACCACG GAGCTTGGGA ACAGAAAACA 
GGTCATGTGA GTCCACTTTA CGGCTTATCA TCGGACACTT ATCCTCAGTA CAATACAAAC 
TATACCATGC AGTTACTCCT AAAAATGGGA GCGCGAAGAG AAAAACTTGT ATTGAGCATT 
CCGTTTTACG GCCAGAGTTT TACATTGGCA ACCGCCCACC AAATTCTTGC AGGCCCAGGA 
GTGGCAGCCT CTGGACCTGG AGATGCCGGT GAATTGACAA AGCAACCTGG AATGCTAGCT 
TACTACGAGA TTTGTCAGCG CCTAACAAAG TTTAATTGGA TAAGTGATCG GAATTTAAAC 
GTGATATTCG GTCCATTCGC TATGCTAAAC GACCAGTGGG TAGGATATGA AGACCCCACC 
AGTGCCCAAG CCAAAGCTCG ATATGCGGCT AACAACAACT TTGCCGGAGT GGCAGCCTGG 
ACCATTGACC TTGATGACTT CCGAAACCTA TGTTGCAATG AATCTTATCC TTTACTTAGG 
GCCATTAATA GAGCATTGGG TCGCTTGGAC TCGGAGCCGC CAACTCAACC GAACTGTAAG 
CGTCCTACAT TATTGGCGAC ACCAGTGCCG CCTCAAATGA CAACAATTAG TAGCGATGGT 
TCTGGGGGAT TGGGACAAAA TCACGATCAT ACAACTTCCT TGCCAAGCGG GCAGATCAGC 
AGTAGTCCTG TAAGTACCAC TATCACGAGC ACCTTCCCCT GGTGGAGTTC AACTACAAAA 
CGTCCGCGCG AGCCGACTAA GACTACAGCA CAACCAACTC ACACCACAAT CCTCATTCCA 
GCAGGGATCA ACCCAGTGGT ACAGCCCTCG AATTGCAAAA GCGGCGAGTT CTTCGCTGAC 
TCAAATAATT GTAACGCTTA CTATCACTGT TTTTTTGCGG GAGAGTTACA GCAACAGTTT 
TGTCCAAGCG GTCTTCACTG GAACAACGAA GCTAAAGGAT GTGACTGGCC GTCGTCCGCC 
CAATGCTCAC TAAAACTCGA TCAACACCTA AGTACTTCTT ATCCAAACCC GATTCAAACG 
TCTAAAAAGC CGGAGACGAC TTTGAAACCT AATAAGAAAC CGTCAGAGAT TTCCACACAT 
CATCAGGTAA ATTCTACCTC AAGCCGACCA CAATATATGC GTCCAACTAT ATTGGAGTGC 
ACTGAAGGTG ATTACTACCC ACACAGAAAC TGCCGGAAAT ATTACATCTG CGTTAATAAG 
GCACTGGTAC CCAGTGAATG TGGCGGAGAC TTGCATTGGG ACGGTATCAA AAAACTTTGT 
GACTGGCCTG AGAACGTACA ATGCGTAACT AGCAAAAAAT ATTTAAAAAT AATCAAATCG 
AGTAGCGCCA ACGAAGAAGA CCCGTGCAAA GGCGAAAAGA GAGTACCCTA TCCAGGAAAC 
TGTTCGAAAT ATCTCTTCTG CCTATGGAAC CGCCTTCAGG CTAGTGACTG TCCACCAGGG 
CTGCACTATA ATGAAAGAAT TGGGAACTGC GATTGGCCAG CAGCTGCAAA ATGCAATCCA 
AAAGGTAGTG AGAGCAGCGA AGAGGCAGAG CTGAACGCTA TGCCGAAGCC TCCGACTCCA 
CAAACCCCCA GTAGTCATTT GCGTCCAACG TACCCCACAG AAAAACCAGT TCCCAAACCT 
CGTGATAGTC ACTACAAAGT TATTTGCTAC TTTACAAACT GGGCCTGGTA CCGCAAAGGA 
ATAGGTCGCT TTACTCCCGA CGACATAAAC ACGGAGCTGT GCACTCACGT AATTTATGGG 
TTTGCAGTGT TAGACTATTC CGAACTTGTA CTACGAACAC ATGATTCCTG GGCTGACGTT 
GAAAACAACT TTTACACAAG GGTCACCAGC CTCAAAAGCA AGGGCATCAA GGTTAGCTTA 
GCACTTGGTG GATGGAACGA TTCGCAAGGC GATAAGTACA GCCGCCTAGT TCGAAGTCCA 
ATGGCCCGGT CCCGTTTTGT GCGCCACGCC CTCGAGTTTA TTGAAAAATA CGGCTTTGAG 
GGCCTTGATT TAGATTGGGA GTACCCAGTC TGCTGGCAAA CGGAGTGCAA CAAAGGGTCT 
ACCGAGGAAA AGGACGGTTT CACTGCCTGG GTACAGGAGC TTTCTGAAGC GTTTCGCCCT 
CGTGGACTTA TGTTGTCCAC TGCTGTCTCG CCTAGCAGGA AAATCATTGA TGCCGGATAC 
GACATCCCTC AGCTCTCTCG CTATTTTGAT TGGATCGCTG TCATGACATA TGATTTTCAT 
GGACATTGGG ATAAAAAAAC AGGACATGTG GCACCGCTGT ATCATCATCC CGATGATGAC 
TTTGAATATT TTAACGTGAA CTATTCTATA AACTATTGGA TGGAAAAAGG AGCACCATCA 
CAAAAATTAG TAATGGGTAT TCCGCTATAC GGCCAGTCGT TCACTTTGGA AAATACCAAC 
AGCAGTGGCT TAAATGCCAA AGCCCCTGCA CCAGGTGAAG CTGGAGAGTT CACCAGAGCA 
GCTGGATTTC TTGCTTACTA TGAGATTTGT GAACGAGTAA ATCGCCAAGG CTGGCAGGTA 
GTGCATGATG AATTTGGGCG CATGGGACCT TATGCTTACA AGGGAACTCA GTGGGTGTCC 
TACGATAGCC CCGATATGGT CAGGAAAAAA TCACTACTGG TGAGGTCATT GAAACTGGGC 
GGTGGAATGG TTTGGGCTCT TGATCTGGAT GACTTTAAAA ATCGGTGTGG AAATGGCGTG 
CATCCTTTGC TCACTGAAAT CCATAACGTG CTTAAGGATC CACCTAGTTT AATGGAAATA 
CCTGGTCCTA TCGAAACGAC TCCTACAGAG TACCCAGGCA TGGAAGAAGA GATACATGAG 
TCTAATGGGG AGGGTCCCGA GGTGCAGCCG ATTGAAGCGG TGATGCAGAC CTGCGAAAAT 
GAAGGTGAAG AACATGAAGG TATTTTAGAC CCAAATCACG TCCTTGAGGA GGAAAACATA 
GAAGCTACTG AAATGGCCAC GGAATTTAAA ATCATTTGCT ACTTTACCAA CTGGGCGTGG 
TACCGTCAAG GTGGTGGTAA GTTCCTGCCG GAAGATATTG ATTCGGATCT GTGTACCCAC 
ATAATTTATG GCTTTGCCGT GTTGAGTCGG GACAACCTTA CGATTCAGCC TCATGACTCT 
TGGGCGGACT TAGACAACAA GTTTTATGAG CGGATTGTGG CCTACCGCAA GAAAGGCGCT 
AAAGTAACTG TGGCAATTGG CGGTTGGAAT GATTCTGCTG GCGATAAGTA CTCCCGACTT 
GTAAGGAATC CAGAGGCAAG ATCTCGGTTT ATCCGTAATG TCTTGGATTT TATAGAGGAA 
TATAATTTCG ACGGCTTGGA CCTGGATTGG GAGTACCCCG TTTGCTGGCA GGTGGATTGC 
AAGAAAGGGA CGGCAGAAGA GAAGATTGGA TTCTCAGCTT TGGTGCGGGA GCTTTTTTAT 
GCCTTCCAGC CCAGGGGATT AATTCTGTCA GCTGCCGTGT CACCTAACAA GAAGGTAATC 
GATGCTGGAT ACGAAGTTGC GGAGCTATCC CATTACTTTT CTTGGATATC TGTGATGGCC 
TATGACTACC ACGGTCAGTG GGATAAGAAG ACTGGCCATG TAGCTCCAAT GTACAGCCAC 
CCAGAAGGAA CAGCAAATTT TAACGCCAAT TTTTCCATGA ACTACTGGAT CTCGATGGGA 
GCTGACCGGC GAAAGTTAGT AATGGGAATA CCTCTGTACG GCCAGTCATT CTCTCTGGCT 
GAGACTACTA AGCACCAACT AAATGCTCCC ACGTATGGTG GCGGTGAAGC TGGAGAGGCT 
ACAAGAGCCC GAGGATTTTT GGCATACTAT GAAATATGCT TGAAAATCCG ACATCATAGG 
TGGAACGTTG TAAGGGATAC CAAGGGTCGA ATAGGACCCT TCGCATATCA TGGGGATCAG 
TGGGTTTCGT TCGATGATGT ACCCATGATT CGGCATAAAA GCGAGTACAT CAAGGCGATG 
GGTCTTGGTG GAGCTATGAT ATGGGCTCTA GACTTGGATG ACTTCAAAAA TGTGTGCGAA 
TGCGAGTCAT ATCCCTTGCT TAAAGCTATT AATCGTGTAC TCAGAGGTTT CGGGGGTCCC 
CAACCGAAGT GCTTACTTGA AAACCCCAAA AGCACAATGA AGCCAAACAT CAAACCGCCT 
TTCAGGCCAA CAATAAATGC CCCATCTGGA CCTAATCTTG ATTCTCCTAC CCAAAATGTA 
TCCTTAAATG TCCTGTCCAT CAAATGCCAC AGCAAAAACT ATTTGGCGCA CGAATGGGAT 
TGCACTAAGT ACTATATCTG CGAACATGGA ACGTACGTCG AGCGAAGCTG TCCTTTGGGA 
CTTCAATGGA ATAAAACCTA CTGCGACTGG CCTACCAATG TTCAGTCCTC ATTGGGATCA 
AACCAGAGAA CTCAGGAGCC AGCGGTCCAT CGCCCCAATC CTACAAGTGT CATTACAGAG 
CCCCCAATCA TCAATAACTC ATACAAAGTA GTCTGTTACT TTACGAGCTG GGCATGGTAC 
CGTTCGTCAC AGGGCAAGTT TGTGCCTGAA GATATAGACG CCAACTTGTG CACCCACCTC 
ATTTACGGCT TCGCGGTGCT TGATAGCAAG TCGTTAACCA TTAAGACGCA TGACTCCTGG 
ACCGATATAG ACAACCGTTT CTATGAGCGA GTTGTAGAAT ACAAGCAAAG AGGCCTTCGA 
GTCATGTTAG CCATTGGTGG ATGGAATGAC TCGTTGGGCA GCAAGTACGC ACGTCTTGTT 
CTTAATTCAC AGTCCAGGAG GCGCTTTGTC GCAAGTGTGA TAAGTTTTCT AGAACAACAC 
GGTTTTGAGG GTCTGGACTT AGCCTGGGAG TTTCCTGTAT GCTGGCAAGT TAACTGCAAC 
AGAGGTAATC CCACGGAAAA GGATGGATTT GTTGCTCTTG TCAAAGAACT GTCGGAAGCC 
TTTAAGGAAA ACGGTCTTAT TCTTTCGGCA GCAGTCTCGC CCAGTAAAAT GGTTATTGAT 
GCCGGGTACA ATGTGTTTGA GCTCTCTCCG TACTTTGACT GGGTGGCCGT CATGACGTAC 
GACTTTCACG GTCACTGGGA TATGCGCACT GGCCAAATAG CCCCACTCTT TCACAGAGGG 
GGTGACGAAA ACCTATACCT TAACGGAAAC TTCAGCATCC ATTACTGGTT GGAAAGGGGT 
ATTCCAAACG ACAAGCTGGT TATGGGAATG CCAATGTACG GACAGACGTT TACGCTAGCT 
GACCAAAATC GCCGGTCACT AAATGATAAA ACAGTGGGTC CGGGGAAGGC AGGAACCTTT 
ACTAGAGCGG ATGGATTCTT GGCGTACTAT GAAATTTGCG AAAAAGTCGT CAACGATGAC 
TGGAAAGTGG TGAGGGATGA AGAAGGTATA TTCGGATCCT ATGCATATAG TGGCAACCAG 
TGGATATCAT ACGATGATGT CACAACTATT CGAAGAAAAT CTCAGTTTAT TAAGAGCTTG 
CAGCTAGGTG GCGGCATGAT TTGGGCCCTT GATTTAGATG ACTTTCGTGG ACTTTGCGGT 
TGCGGCAAGC ACCCATTACT CAGGACTCTT AGTCAGGAGC TCCTAGGCAT TCCTGGACAA 
AAGGCTAAGG ACTGCATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cht10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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