Snap25 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Snap25
Entrez Gene ID	3355084
Full Name	Synaptosomal-associated protein 25kDa
Synonyms	CG17676,CG17884,CG40452,Dmel\CG40452,SNAP-25,SNAP25,dSNAP-25,snap-25,snap25,snp25
General protein information	Preferred Names Synaptosomal-associated protein 25kDa Names CG40452 gene product from transcript CG40452-RA CG40452-PA CG40452-PC Snap25-PA Snap25-PC synapse protein 25 synaptosomal-associated protein 25
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043176.2	NP_001036641.1	Synaptosomal-associated protein 25kDa, isoform A
NM_001043177.3	NP_001036642.2	Synaptosomal-associated protein 25kDa, isoform C

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100491227	XP_002938631.1
Danio rerio (zebrafish)	zgc:101731	NP_001020729.1
Drosophila melanogaster (fruit fly)	Snap25	NP_001036642.2
Caenorhabditis elegans (roundworm)	ric-4	NP_505641.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001394	XP_321742.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results indicate that SNAP-25-R206 and syntaxin-D253 play a major role in neuroexocytosis and support a radial assembly of several SNARE complexes interacting via the ionic couple formed by these two residues.
Title: Evidence for a radial SNARE super-complex mediating neurotransmitter release at the Drosophila neuromuscular junction.

Results establish in vivo contributions of SNAP-25 and NSF to synaptic vesicle trafficking and define molecular mechanisms determining conserved functional properties of short-term depression.
Title: Molecular mechanisms determining conserved properties of short-term synaptic depression revealed in NSF and SNAP-25 conditional mutants.

study found dHIP14 to be an essential maternal effect gene required for photoreceptor synaptic transmission and for proper in vivo expression of the palmitoylated presynaptic proteins SNAP-25 and cysteine string protein
Title: Drosophila huntingtin-interacting protein 14 is a presynaptic protein required for photoreceptor synaptic transmission and expression of the palmitoylated proteins synaptosome-associated protein 25 and cysteine string protein.

reveals context-dependent redundancy with SNAP-24 in neurotransmission
Title: A Drosophila SNAP-25 null mutant reveals context-dependent redundancy with SNAP-24 in neurotransmission.

The following Snap25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Snap25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20103	NM_001043176.2 Latest version!	Drosophila melanogaster Synaptosomal-associated protein 25kDa (Snap25), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa20104	NM_001043177.3 Latest version!	Drosophila melanogaster Synaptosomal-associated protein 25kDa (Snap25), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Snap25 ( NM_001043176.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036641.1 Drosophila melanogaster Synaptosomal-associated protein 25kDa (Snap25), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa20103
Clone ID Related Accession (Same CDS sequence)	NM_001043176.2
Accession Version	NM_001043176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Synaptosomal-associated protein 25kDa, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001043176.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGCGG ATCCATCTGA AGAAGTTGCC CCTCAGGTCC CGAAGACCGA GCTAGAAGAG 
CTGCAAATTA ATGCGCAAGG AGTAGCCGAT GAGTCCCTGG AAAGTACGCG ACGTATGCTT 
GCTCTGTGTG AGGAGAGCAA GGAGGCAGGG ATTCGAACAC TTGTAGCCCT TGATGATCAA 
GGAGAACAAC TGGATCGTAT TGAAGAAGGA ATGGATCAAA TTAATGCAGA CATGAGAGAA 
GCAGAAAAAA ATTTAAGTGG AATGGAAAAA TGTTGCGGCA TTTGTGTTCT TCCGTGCAAT 
AAAAGTCAAT CATTCAAAGA AGATGATGGG ACCTGGAAAG GAAATGATGA CGGAAAAGTT 
GTAAATAATC AGCCACAGAG AGTGATGGAT GATAGAAATG GCATGATGGC GCAAGCGGGT 
TATATTGGCA GGATAACGAA CGACGCTAGA GAAGATGAAA TGGAAGAAAA TATGGGCCAG 
GTAAACACTA TGATAGGCAA TCTCCGAAAT ATGGCATTGG ATATGGGCTC TGAGCTGGAA 
AATCAAAATC GTCAAATTGA TAGAATAAAC CGGAAGGGTG AATCTAATGA AGCGCGGATA 
GCAGTTGCTA ATCAAAGGGC ACATCAACTA TTAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036641.1
CDS	133..771
Translation	MPADPSEEVAPQVPKTELEELQINAQGVADESLESTRRMLALCEESKEAGIRTLVALDDQGEQLDRIEEGMDQINADMREAEKNLSGMEKCCGICVLPCNKSQSFKEDDGTWKGNDDGKVVNNQPQRVMDDRNGMMAQAGYIGRITNDAREDEMEENMGQVNTMIGNLRNMALDMGSELENQNRQIDRINRKGESNEARIAVANQRAHQLLK

Target ORF information:

RefSeq Version	NM_001043176.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Synaptosomal-associated protein 25kDa (Snap25), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043176.2

ORF Insert Sequence:

ATGCCAGCGG ATCCATCTGA AGAAGTTGCC CCTCAGGTCC CGAAGACCGA GCTAGAAGAG 
CTGCAAATTA ATGCGCAAGG AGTAGCCGAT GAGTCCCTGG AAAGTACGCG ACGTATGCTT 
GCTCTGTGTG AGGAGAGCAA GGAGGCAGGG ATTCGAACAC TTGTAGCCCT TGATGATCAA 
GGAGAACAAC TGGATCGTAT TGAAGAAGGA ATGGATCAAA TTAATGCAGA CATGAGAGAA 
GCAGAAAAAA ATTTAAGTGG AATGGAAAAA TGTTGCGGCA TTTGTGTTCT TCCGTGCAAT 
AAAAGTCAAT CATTCAAAGA AGATGATGGG ACCTGGAAAG GAAATGATGA CGGAAAAGTT 
GTAAATAATC AGCCACAGAG AGTGATGGAT GATAGAAATG GCATGATGGC GCAAGCGGGT 
TATATTGGCA GGATAACGAA CGACGCTAGA GAAGATGAAA TGGAAGAAAA TATGGGCCAG 
GTAAACACTA TGATAGGCAA TCTCCGAAAT ATGGCATTGG ATATGGGCTC TGAGCTGGAA 
AATCAAAATC GTCAAATTGA TAGAATAAAC CGGAAGGGTG AATCTAATGA AGCGCGGATA 
GCAGTTGCTA ATCAAAGGGC ACATCAACTA TTAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Snap25 ( NM_001043177.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036642.2 Drosophila melanogaster Synaptosomal-associated protein 25kDa (Snap25), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa20104
Clone ID Related Accession (Same CDS sequence)	NM_001043177.3
Accession Version	NM_001043177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Synaptosomal-associated protein 25kDa, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001043177.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGCGG ATCCATCTGA AGAAGTTGCC CCTCAGGTCC CGAAGACCGA GCTAGAAGAG 
CTGCAAATTA ATGCGCAAGG AGTAGCCGAT GAGTCCCTGG AAAGTACGCG ACGTATGCTT 
GCTCTGTGTG AGGAGAGCGC GGAAGTTGGA ATGCGAAGTA TTGTTATGTT GGATGAGCAA 
GGAGAACAAC TGGATCGTAT TGAAGAAGGA ATGGATCAAA TTAATGCAGA CATGAGAGAA 
GCAGAAAAAA ATTTAAGTGG AATGGAAAAA TGTTGCGGCA TTTGTGTTCT TCCGTGCAAT 
AAAAGTCAAT CATTCAAAGA AGATGATGGG ACCTGGAAAG GAAATGATGA CGGAAAAGTT 
GTAAATAATC AGCCACAGAG AGTGATGGAT GATAGAAATG GCATGATGGC GCAAGCGGGT 
TATATTGGCA GGATAACGAA CGACGCTAGA GAAGATGAAA TGGAAGAAAA TATGGGCCAG 
GTAAACACTA TGATAGGCAA TCTCCGAAAT ATGGCATTGG ATATGGGCTC TGAGCTGGAA 
AATCAAAATC GTCAAATTGA TAGAATAAAC CGGAAGGGTG AATCTAATGA AGCGCGGATA 
GCAGTTGCTA ATCAAAGGGC ACATCAACTA TTAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036642.2
CDS	133..771
Translation	MPADPSEEVAPQVPKTELEELQINAQGVADESLESTRRMLALCEESAEVGMRSIVMLDEQGEQLDRIEEGMDQINADMREAEKNLSGMEKCCGICVLPCNKSQSFKEDDGTWKGNDDGKVVNNQPQRVMDDRNGMMAQAGYIGRITNDAREDEMEENMGQVNTMIGNLRNMALDMGSELENQNRQIDRINRKGESNEARIAVANQRAHQLLK

Target ORF information:

RefSeq Version	NM_001043177.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Synaptosomal-associated protein 25kDa (Snap25), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043177.3

ORF Insert Sequence:

ATGCCAGCGG ATCCATCTGA AGAAGTTGCC CCTCAGGTCC CGAAGACCGA GCTAGAAGAG 
CTGCAAATTA ATGCGCAAGG AGTAGCCGAT GAGTCCCTGG AAAGTACGCG ACGTATGCTT 
GCTCTGTGTG AGGAGAGCGC GGAAGTTGGA ATGCGAAGTA TTGTTATGTT GGATGAGCAA 
GGAGAACAAC TGGATCGTAT TGAAGAAGGA ATGGATCAAA TTAATGCAGA CATGAGAGAA 
GCAGAAAAAA ATTTAAGTGG AATGGAAAAA TGTTGCGGCA TTTGTGTTCT TCCGTGCAAT 
AAAAGTCAAT CATTCAAAGA AGATGATGGG ACCTGGAAAG GAAATGATGA CGGAAAAGTT 
GTAAATAATC AGCCACAGAG AGTGATGGAT GATAGAAATG GCATGATGGC GCAAGCGGGT 
TATATTGGCA GGATAACGAA CGACGCTAGA GAAGATGAAA TGGAAGAAAA TATGGGCCAG 
GTAAACACTA TGATAGGCAA TCTCCGAAAT ATGGCATTGG ATATGGGCTC TGAGCTGGAA 
AATCAAAATC GTCAAATTGA TAGAATAAAC CGGAAGGGTG AATCTAATGA AGCGCGGATA 
GCAGTTGCTA ATCAAAGGGC ACATCAACTA TTAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Snap25 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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