Vmat cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vmat
Entrez Gene ID	3346192
Full Name	Vesicular monoamine transporter
Synonyms	CG33528,CG6119,CG6139,DVMAT,DVMAT-A,DVMAT-B,Dmel\CG33528,VMAT,dVMAT,l(2)SH0459,l(2)SH2 0459,vmat
General protein information	Preferred Names Vesicular monoamine transporter Names CG33528 gene product from transcript CG33528-RF CG33528-PC CG33528-PD CG33528-PE CG33528-PF Vmat-PC Vmat-PD Vmat-PE Vmat-PF drosophila vesicular monoamine transporter lethal (2) SH0459 vesicle transporter vesicular monoamine transporter
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50B2-50B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274027.2	NP_001260956.1	vesicular monoamine transporter, isoform F
NM_001014524.2	NP_001014524.1	vesicular monoamine transporter, isoform C
NM_001014526.2	NP_001014526.1	vesicular monoamine transporter, isoform E
NM_001014525.3	NP_001014525.1	vesicular monoamine transporter, isoform D

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLC18A2	XP_521615.3
Bos taurus (cattle)	SLC18A2	NP_777078.1
Rattus norvegicus (Norway rat)	Slc18a2	NP_037163.1
Gallus gallus (chicken)	SLC18A2	XP_421782.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004476	XP_313775.4
Caenorhabditis elegans (roundworm)	cat-1	NP_001024936.1
Homo sapiens (human)	SLC18A2	NP_003045.2
Mus musculus (house mouse)	Slc18a2	NP_766111.1
	SLC18A2	XP_001095699.2
Canis lupus familiaris (dog)	SLC18A2	XP_544038.2
Danio rerio (zebrafish)	slc18a2	NP_001243154.1
Drosophila melanogaster (fruit fly)	Vmat	NP_001014524.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study identify VMAT as an important regulator of sleep in Drosophila and demonstrate that small-molecule screens provide an effective approach to identify genes and pathways that impact adult Drosophila behavior.
Title: Small-molecule screen in adult Drosophila identifies VMAT as a regulator of sleep.

Interaction between vesicular monoamine transporter and pesticides in vivo does not increase survival of fruit flies exposed to rotenone but blocks the loss of dopaminergic neurons caused by the pesticide.
Title: The Drosophila vesicular monoamine transporter reduces pesticide-induced loss of dopaminergic neurons.

A novel mRNA splice variant of Drosophila VMAT (DVMAT-B) is expressed not in neurons but rather in a small subset of glia in the lamina of the fly's optic lobe.
Title: A glial variant of the vesicular monoamine transporter is required to store histamine in the Drosophila visual system.

The following Vmat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vmat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11809	NM_001014524.2 Latest version!	Drosophila melanogaster vesicular monoamine transporter, transcript variant C (Vmat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11809	NM_001274027.2 Latest version!	Drosophila melanogaster vesicular monoamine transporter, transcript variant F (Vmat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11809	NM_001014526.2 Latest version!	Drosophila melanogaster vesicular monoamine transporter, transcript variant E (Vmat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11810	NM_001014525.3 Latest version!	Drosophila melanogaster vesicular monoamine transporter, transcript variant D (Vmat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vmat ( NM_001274027.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260956.1 Drosophila melanogaster vesicular monoamine transporter, transcript variant F (Vmat), mRNA.

Price & Availability↑

CloneID	OFa11809
Clone ID Related Accession (Same CDS sequence)	NM_001274027.2 , NM_001014524.2 , NM_001014526.2
Accession Version	NM_001274027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vesicular monoamine transporter, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274027.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCAT CGACCGATGC GGGCAATGGT GGACCAAGAA AACACACGCA GAGCACAGCC 
CCCCAGAAGC AGTCGGAGAT TAGCGAGACC ACCTCCTTCA CCGTAAACGC AAACACCACC 
ACCAACAGCC AGCAGCAACA GATTCCCGGC GGACCCGCCA GCAATACGCC CAGCAAAAAT 
CCCTTCAAGC AGCAGTTGGA CAGCAATCAA CAGAATGGCA ATATGGAGCC GGATTGCGGC 
ATCATGGGCG TCGGCAAGGA GCCACCGGTA CCGCCGCCGC CCACCTACCA GTCGCAGACC 
TACGCAGGAC GACAGGCTCC GCCGCCAGCG GATCAGTACC GCGAGGAGGA TCCACGCGCT 
GCCGGGTCCT GGGCCGCCGC CAAATCCTGG ATGGTCAGCT GGCGGGGATC CAATCGCCTG 
GTGCTCGTCA TCGTGGCCAT AGCCCTGCTC CTCGACAACA TGCTGCTGAC CACAGTGGTG 
CCCATCATAC CCGAGTTCCT GTACGACATA CGACATCCGG ATGCGCCGCT CGACAGCTTT 
CCACGCACAC CTTTAACGTC CAACGTTCCG CCGCCACCGA CGCCGTGTCC ATGCAACAAG 
GATGGCAGCG AGGCGGCTCC GCTCGAGATC TCCACGATTA GTCCGGAGGA GAACGAGACC 
TATTACCGGG AACTGGAGGA GCGGCACAAC GAGCTGGTGG GCGAGACCGT CGAGGTGGGA 
CTTCTATTTG CCTCCAAGGC GTTTGTCCAG CTGCTGGTTA ACCCAATTGT TGGACCCCTG 
ACTCACAGGA TTGGCTATAG TATTCCCATG TTCGCTGGCT TTGTGATAAT GTTCCTCTCA 
ACGATAATTT TCGCTTTTGG ACGATCTTAC CTGGTACTGT TCGTGGCGAG GGCACTGCAG 
GGCATTGGAT CATCCTGCTC CTCGGTTTCC GGAATGGGCA TGCTGGCAGA TCGATTTACG 
GACGACAAGG AGCGTGGAAA TGCCATGGGC ATAGCACTGG GAGGATTGGC TTTGGGCGTG 
CTCATCGGTC CACCATTCGG TGGCGTGATG TACGAGTTTG TGGGCAAGTC GGCTCCATTC 
CTGATCCTGG CTGCCCTGGC CTTGGGCGAT GGCCTGCTAC AGTTGTTCAT GCTGCAGCCA 
TCGATCCAGA AGGCCGAAAC GGAACCGCCC TCGCTGAAGA GCCTCATCAG TGATCCCTAC 
ATCCTGATTG CTGCTGGTGC CATCACGTTC GCCAATATGG GCATTGCCAT GCTGGAACCA 
TCGCTACCGC TGTGGATGGT GGACAACATG GGCGCCACTC GTTGGGAGCA GGGCGTGGCC 
TTCCTGCCCG CCTCCATTAG CTACCTGATT GGCACCAATC TCTTCGGACC GCTGGGACAC 
AAAATTGGAC GATGGTTTGC CGCGTGCTTG GGATTGATTA TCATCGGGGG ATGCTTGATA 
TTTATACCCA TGGCCACTTC GATCACTCAC CTGATCATCC CGAATGCAGG ACTTGGCTTT 
GCCATTGGAA TGGTGGACTC GTCGATGATG CCGGAACTGG GCTATCTGGT GGACATCCGA 
CACTCGGCGG TCTACGGCAG CGTTTACGCC CTGGGTGATG TGGCCTTCTG TGTTGGCTTC 
GCCGTGGGTC CCGCCTTATC TGGATCGCTG GTGAAGAGCA TCGGCTTCGA GTGGATGTTG 
TTTGGAATTG CCATACTCTG CTTTATGTAT GCTCCATTGC TGACGCTGCT CAAGAATCCG 
CCGACCAGCG ACGAGAAGAA GTCTTTGATC TATGGACGCG ATCGGGCTCA GGTACGTTAT 
GTAACCTATC AAAACTACGA GGAAGACGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260956.1
CDS	373..2205
Translation	MQSSTDAGNGGPRKHTQSTAPQKQSEISETTSFTVNANTTTNSQQQQIPGGPASNTPSKNPFKQQLDSNQQNGNMEPDCGIMGVGKEPPVPPPPTYQSQTYAGRQAPPPADQYREEDPRAAGSWAAAKSWMVSWRGSNRLVLVIVAIALLLDNMLLTTVVPIIPEFLYDIRHPDAPLDSFPRTPLTSNVPPPPTPCPCNKDGSEAAPLEISTISPEENETYYRELEERHNELVGETVEVGLLFASKAFVQLLVNPIVGPLTHRIGYSIPMFAGFVIMFLSTIIFAFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRFTDDKERGNAMGIALGGLALGVLIGPPFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKAETEPPSLKSLISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIGRWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPLLTLLKNPPTSDEKKSLIYGRDRAQVRYVTYQNYEEDE

Target ORF information:

RefSeq Version	NM_001274027.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vesicular monoamine transporter, transcript variant F (Vmat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274027.2

ORF Insert Sequence:

ATGCAATCAT CGACCGATGC GGGCAATGGT GGACCAAGAA AACACACGCA GAGCACAGCC 
CCCCAGAAGC AGTCGGAGAT TAGCGAGACC ACCTCCTTCA CCGTAAACGC AAACACCACC 
ACCAACAGCC AGCAGCAACA GATTCCCGGC GGACCCGCCA GCAATACGCC CAGCAAAAAT 
CCCTTCAAGC AGCAGTTGGA CAGCAATCAA CAGAATGGCA ATATGGAGCC GGATTGCGGC 
ATCATGGGCG TCGGCAAGGA GCCACCGGTA CCGCCGCCGC CCACCTACCA GTCGCAGACC 
TACGCAGGAC GACAGGCTCC GCCGCCAGCG GATCAGTACC GCGAGGAGGA TCCACGCGCT 
GCCGGGTCCT GGGCCGCCGC CAAATCCTGG ATGGTCAGCT GGCGGGGATC CAATCGCCTG 
GTGCTCGTCA TCGTGGCCAT AGCCCTGCTC CTCGACAACA TGCTGCTGAC CACAGTGGTG 
CCCATCATAC CCGAGTTCCT GTACGACATA CGACATCCGG ATGCGCCGCT CGACAGCTTT 
CCACGCACAC CTTTAACGTC CAACGTTCCG CCGCCACCGA CGCCGTGTCC ATGCAACAAG 
GATGGCAGCG AGGCGGCTCC GCTCGAGATC TCCACGATTA GTCCGGAGGA GAACGAGACC 
TATTACCGGG AACTGGAGGA GCGGCACAAC GAGCTGGTGG GCGAGACCGT CGAGGTGGGA 
CTTCTATTTG CCTCCAAGGC GTTTGTCCAG CTGCTGGTTA ACCCAATTGT TGGACCCCTG 
ACTCACAGGA TTGGCTATAG TATTCCCATG TTCGCTGGCT TTGTGATAAT GTTCCTCTCA 
ACGATAATTT TCGCTTTTGG ACGATCTTAC CTGGTACTGT TCGTGGCGAG GGCACTGCAG 
GGCATTGGAT CATCCTGCTC CTCGGTTTCC GGAATGGGCA TGCTGGCAGA TCGATTTACG 
GACGACAAGG AGCGTGGAAA TGCCATGGGC ATAGCACTGG GAGGATTGGC TTTGGGCGTG 
CTCATCGGTC CACCATTCGG TGGCGTGATG TACGAGTTTG TGGGCAAGTC GGCTCCATTC 
CTGATCCTGG CTGCCCTGGC CTTGGGCGAT GGCCTGCTAC AGTTGTTCAT GCTGCAGCCA 
TCGATCCAGA AGGCCGAAAC GGAACCGCCC TCGCTGAAGA GCCTCATCAG TGATCCCTAC 
ATCCTGATTG CTGCTGGTGC CATCACGTTC GCCAATATGG GCATTGCCAT GCTGGAACCA 
TCGCTACCGC TGTGGATGGT GGACAACATG GGCGCCACTC GTTGGGAGCA GGGCGTGGCC 
TTCCTGCCCG CCTCCATTAG CTACCTGATT GGCACCAATC TCTTCGGACC GCTGGGACAC 
AAAATTGGAC GATGGTTTGC CGCGTGCTTG GGATTGATTA TCATCGGGGG ATGCTTGATA 
TTTATACCCA TGGCCACTTC GATCACTCAC CTGATCATCC CGAATGCAGG ACTTGGCTTT 
GCCATTGGAA TGGTGGACTC GTCGATGATG CCGGAACTGG GCTATCTGGT GGACATCCGA 
CACTCGGCGG TCTACGGCAG CGTTTACGCC CTGGGTGATG TGGCCTTCTG TGTTGGCTTC 
GCCGTGGGTC CCGCCTTATC TGGATCGCTG GTGAAGAGCA TCGGCTTCGA GTGGATGTTG 
TTTGGAATTG CCATACTCTG CTTTATGTAT GCTCCATTGC TGACGCTGCT CAAGAATCCG 
CCGACCAGCG ACGAGAAGAA GTCTTTGATC TATGGACGCG ATCGGGCTCA GGTACGTTAT 
GTAACCTATC AAAACTACGA GGAAGACGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vmat ( NM_001014524.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014524.1 Drosophila melanogaster vesicular monoamine transporter, transcript variant C (Vmat), mRNA.

Price & Availability↑

CloneID	OFa11809
Clone ID Related Accession (Same CDS sequence)	NM_001274027.2 , NM_001014524.2 , NM_001014526.2
Accession Version	NM_001014524.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vesicular monoamine transporter, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001014524.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCAT CGACCGATGC GGGCAATGGT GGACCAAGAA AACACACGCA GAGCACAGCC 
CCCCAGAAGC AGTCGGAGAT TAGCGAGACC ACCTCCTTCA CCGTAAACGC AAACACCACC 
ACCAACAGCC AGCAGCAACA GATTCCCGGC GGACCCGCCA GCAATACGCC CAGCAAAAAT 
CCCTTCAAGC AGCAGTTGGA CAGCAATCAA CAGAATGGCA ATATGGAGCC GGATTGCGGC 
ATCATGGGCG TCGGCAAGGA GCCACCGGTA CCGCCGCCGC CCACCTACCA GTCGCAGACC 
TACGCAGGAC GACAGGCTCC GCCGCCAGCG GATCAGTACC GCGAGGAGGA TCCACGCGCT 
GCCGGGTCCT GGGCCGCCGC CAAATCCTGG ATGGTCAGCT GGCGGGGATC CAATCGCCTG 
GTGCTCGTCA TCGTGGCCAT AGCCCTGCTC CTCGACAACA TGCTGCTGAC CACAGTGGTG 
CCCATCATAC CCGAGTTCCT GTACGACATA CGACATCCGG ATGCGCCGCT CGACAGCTTT 
CCACGCACAC CTTTAACGTC CAACGTTCCG CCGCCACCGA CGCCGTGTCC ATGCAACAAG 
GATGGCAGCG AGGCGGCTCC GCTCGAGATC TCCACGATTA GTCCGGAGGA GAACGAGACC 
TATTACCGGG AACTGGAGGA GCGGCACAAC GAGCTGGTGG GCGAGACCGT CGAGGTGGGA 
CTTCTATTTG CCTCCAAGGC GTTTGTCCAG CTGCTGGTTA ACCCAATTGT TGGACCCCTG 
ACTCACAGGA TTGGCTATAG TATTCCCATG TTCGCTGGCT TTGTGATAAT GTTCCTCTCA 
ACGATAATTT TCGCTTTTGG ACGATCTTAC CTGGTACTGT TCGTGGCGAG GGCACTGCAG 
GGCATTGGAT CATCCTGCTC CTCGGTTTCC GGAATGGGCA TGCTGGCAGA TCGATTTACG 
GACGACAAGG AGCGTGGAAA TGCCATGGGC ATAGCACTGG GAGGATTGGC TTTGGGCGTG 
CTCATCGGTC CACCATTCGG TGGCGTGATG TACGAGTTTG TGGGCAAGTC GGCTCCATTC 
CTGATCCTGG CTGCCCTGGC CTTGGGCGAT GGCCTGCTAC AGTTGTTCAT GCTGCAGCCA 
TCGATCCAGA AGGCCGAAAC GGAACCGCCC TCGCTGAAGA GCCTCATCAG TGATCCCTAC 
ATCCTGATTG CTGCTGGTGC CATCACGTTC GCCAATATGG GCATTGCCAT GCTGGAACCA 
TCGCTACCGC TGTGGATGGT GGACAACATG GGCGCCACTC GTTGGGAGCA GGGCGTGGCC 
TTCCTGCCCG CCTCCATTAG CTACCTGATT GGCACCAATC TCTTCGGACC GCTGGGACAC 
AAAATTGGAC GATGGTTTGC CGCGTGCTTG GGATTGATTA TCATCGGGGG ATGCTTGATA 
TTTATACCCA TGGCCACTTC GATCACTCAC CTGATCATCC CGAATGCAGG ACTTGGCTTT 
GCCATTGGAA TGGTGGACTC GTCGATGATG CCGGAACTGG GCTATCTGGT GGACATCCGA 
CACTCGGCGG TCTACGGCAG CGTTTACGCC CTGGGTGATG TGGCCTTCTG TGTTGGCTTC 
GCCGTGGGTC CCGCCTTATC TGGATCGCTG GTGAAGAGCA TCGGCTTCGA GTGGATGTTG 
TTTGGAATTG CCATACTCTG CTTTATGTAT GCTCCATTGC TGACGCTGCT CAAGAATCCG 
CCGACCAGCG ACGAGAAGAA GTCTTTGATC TATGGACGCG ATCGGGCTCA GGTACGTTAT 
GTAACCTATC AAAACTACGA GGAAGACGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014524.1
CDS	557..2389
Translation	MQSSTDAGNGGPRKHTQSTAPQKQSEISETTSFTVNANTTTNSQQQQIPGGPASNTPSKNPFKQQLDSNQQNGNMEPDCGIMGVGKEPPVPPPPTYQSQTYAGRQAPPPADQYREEDPRAAGSWAAAKSWMVSWRGSNRLVLVIVAIALLLDNMLLTTVVPIIPEFLYDIRHPDAPLDSFPRTPLTSNVPPPPTPCPCNKDGSEAAPLEISTISPEENETYYRELEERHNELVGETVEVGLLFASKAFVQLLVNPIVGPLTHRIGYSIPMFAGFVIMFLSTIIFAFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRFTDDKERGNAMGIALGGLALGVLIGPPFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKAETEPPSLKSLISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIGRWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPLLTLLKNPPTSDEKKSLIYGRDRAQVRYVTYQNYEEDE

Target ORF information:

RefSeq Version	NM_001014524.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vesicular monoamine transporter, transcript variant C (Vmat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014524.2

ORF Insert Sequence:

ATGCAATCAT CGACCGATGC GGGCAATGGT GGACCAAGAA AACACACGCA GAGCACAGCC 
CCCCAGAAGC AGTCGGAGAT TAGCGAGACC ACCTCCTTCA CCGTAAACGC AAACACCACC 
ACCAACAGCC AGCAGCAACA GATTCCCGGC GGACCCGCCA GCAATACGCC CAGCAAAAAT 
CCCTTCAAGC AGCAGTTGGA CAGCAATCAA CAGAATGGCA ATATGGAGCC GGATTGCGGC 
ATCATGGGCG TCGGCAAGGA GCCACCGGTA CCGCCGCCGC CCACCTACCA GTCGCAGACC 
TACGCAGGAC GACAGGCTCC GCCGCCAGCG GATCAGTACC GCGAGGAGGA TCCACGCGCT 
GCCGGGTCCT GGGCCGCCGC CAAATCCTGG ATGGTCAGCT GGCGGGGATC CAATCGCCTG 
GTGCTCGTCA TCGTGGCCAT AGCCCTGCTC CTCGACAACA TGCTGCTGAC CACAGTGGTG 
CCCATCATAC CCGAGTTCCT GTACGACATA CGACATCCGG ATGCGCCGCT CGACAGCTTT 
CCACGCACAC CTTTAACGTC CAACGTTCCG CCGCCACCGA CGCCGTGTCC ATGCAACAAG 
GATGGCAGCG AGGCGGCTCC GCTCGAGATC TCCACGATTA GTCCGGAGGA GAACGAGACC 
TATTACCGGG AACTGGAGGA GCGGCACAAC GAGCTGGTGG GCGAGACCGT CGAGGTGGGA 
CTTCTATTTG CCTCCAAGGC GTTTGTCCAG CTGCTGGTTA ACCCAATTGT TGGACCCCTG 
ACTCACAGGA TTGGCTATAG TATTCCCATG TTCGCTGGCT TTGTGATAAT GTTCCTCTCA 
ACGATAATTT TCGCTTTTGG ACGATCTTAC CTGGTACTGT TCGTGGCGAG GGCACTGCAG 
GGCATTGGAT CATCCTGCTC CTCGGTTTCC GGAATGGGCA TGCTGGCAGA TCGATTTACG 
GACGACAAGG AGCGTGGAAA TGCCATGGGC ATAGCACTGG GAGGATTGGC TTTGGGCGTG 
CTCATCGGTC CACCATTCGG TGGCGTGATG TACGAGTTTG TGGGCAAGTC GGCTCCATTC 
CTGATCCTGG CTGCCCTGGC CTTGGGCGAT GGCCTGCTAC AGTTGTTCAT GCTGCAGCCA 
TCGATCCAGA AGGCCGAAAC GGAACCGCCC TCGCTGAAGA GCCTCATCAG TGATCCCTAC 
ATCCTGATTG CTGCTGGTGC CATCACGTTC GCCAATATGG GCATTGCCAT GCTGGAACCA 
TCGCTACCGC TGTGGATGGT GGACAACATG GGCGCCACTC GTTGGGAGCA GGGCGTGGCC 
TTCCTGCCCG CCTCCATTAG CTACCTGATT GGCACCAATC TCTTCGGACC GCTGGGACAC 
AAAATTGGAC GATGGTTTGC CGCGTGCTTG GGATTGATTA TCATCGGGGG ATGCTTGATA 
TTTATACCCA TGGCCACTTC GATCACTCAC CTGATCATCC CGAATGCAGG ACTTGGCTTT 
GCCATTGGAA TGGTGGACTC GTCGATGATG CCGGAACTGG GCTATCTGGT GGACATCCGA 
CACTCGGCGG TCTACGGCAG CGTTTACGCC CTGGGTGATG TGGCCTTCTG TGTTGGCTTC 
GCCGTGGGTC CCGCCTTATC TGGATCGCTG GTGAAGAGCA TCGGCTTCGA GTGGATGTTG 
TTTGGAATTG CCATACTCTG CTTTATGTAT GCTCCATTGC TGACGCTGCT CAAGAATCCG 
CCGACCAGCG ACGAGAAGAA GTCTTTGATC TATGGACGCG ATCGGGCTCA GGTACGTTAT 
GTAACCTATC AAAACTACGA GGAAGACGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vmat ( NM_001014526.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014526.1 Drosophila melanogaster vesicular monoamine transporter, transcript variant E (Vmat), mRNA.

Price & Availability↑

CloneID	OFa11809
Clone ID Related Accession (Same CDS sequence)	NM_001274027.2 , NM_001014524.2 , NM_001014526.2
Accession Version	NM_001014526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vesicular monoamine transporter, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001014526.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCAT CGACCGATGC GGGCAATGGT GGACCAAGAA AACACACGCA GAGCACAGCC 
CCCCAGAAGC AGTCGGAGAT TAGCGAGACC ACCTCCTTCA CCGTAAACGC AAACACCACC 
ACCAACAGCC AGCAGCAACA GATTCCCGGC GGACCCGCCA GCAATACGCC CAGCAAAAAT 
CCCTTCAAGC AGCAGTTGGA CAGCAATCAA CAGAATGGCA ATATGGAGCC GGATTGCGGC 
ATCATGGGCG TCGGCAAGGA GCCACCGGTA CCGCCGCCGC CCACCTACCA GTCGCAGACC 
TACGCAGGAC GACAGGCTCC GCCGCCAGCG GATCAGTACC GCGAGGAGGA TCCACGCGCT 
GCCGGGTCCT GGGCCGCCGC CAAATCCTGG ATGGTCAGCT GGCGGGGATC CAATCGCCTG 
GTGCTCGTCA TCGTGGCCAT AGCCCTGCTC CTCGACAACA TGCTGCTGAC CACAGTGGTG 
CCCATCATAC CCGAGTTCCT GTACGACATA CGACATCCGG ATGCGCCGCT CGACAGCTTT 
CCACGCACAC CTTTAACGTC CAACGTTCCG CCGCCACCGA CGCCGTGTCC ATGCAACAAG 
GATGGCAGCG AGGCGGCTCC GCTCGAGATC TCCACGATTA GTCCGGAGGA GAACGAGACC 
TATTACCGGG AACTGGAGGA GCGGCACAAC GAGCTGGTGG GCGAGACCGT CGAGGTGGGA 
CTTCTATTTG CCTCCAAGGC GTTTGTCCAG CTGCTGGTTA ACCCAATTGT TGGACCCCTG 
ACTCACAGGA TTGGCTATAG TATTCCCATG TTCGCTGGCT TTGTGATAAT GTTCCTCTCA 
ACGATAATTT TCGCTTTTGG ACGATCTTAC CTGGTACTGT TCGTGGCGAG GGCACTGCAG 
GGCATTGGAT CATCCTGCTC CTCGGTTTCC GGAATGGGCA TGCTGGCAGA TCGATTTACG 
GACGACAAGG AGCGTGGAAA TGCCATGGGC ATAGCACTGG GAGGATTGGC TTTGGGCGTG 
CTCATCGGTC CACCATTCGG TGGCGTGATG TACGAGTTTG TGGGCAAGTC GGCTCCATTC 
CTGATCCTGG CTGCCCTGGC CTTGGGCGAT GGCCTGCTAC AGTTGTTCAT GCTGCAGCCA 
TCGATCCAGA AGGCCGAAAC GGAACCGCCC TCGCTGAAGA GCCTCATCAG TGATCCCTAC 
ATCCTGATTG CTGCTGGTGC CATCACGTTC GCCAATATGG GCATTGCCAT GCTGGAACCA 
TCGCTACCGC TGTGGATGGT GGACAACATG GGCGCCACTC GTTGGGAGCA GGGCGTGGCC 
TTCCTGCCCG CCTCCATTAG CTACCTGATT GGCACCAATC TCTTCGGACC GCTGGGACAC 
AAAATTGGAC GATGGTTTGC CGCGTGCTTG GGATTGATTA TCATCGGGGG ATGCTTGATA 
TTTATACCCA TGGCCACTTC GATCACTCAC CTGATCATCC CGAATGCAGG ACTTGGCTTT 
GCCATTGGAA TGGTGGACTC GTCGATGATG CCGGAACTGG GCTATCTGGT GGACATCCGA 
CACTCGGCGG TCTACGGCAG CGTTTACGCC CTGGGTGATG TGGCCTTCTG TGTTGGCTTC 
GCCGTGGGTC CCGCCTTATC TGGATCGCTG GTGAAGAGCA TCGGCTTCGA GTGGATGTTG 
TTTGGAATTG CCATACTCTG CTTTATGTAT GCTCCATTGC TGACGCTGCT CAAGAATCCG 
CCGACCAGCG ACGAGAAGAA GTCTTTGATC TATGGACGCG ATCGGGCTCA GGTACGTTAT 
GTAACCTATC AAAACTACGA GGAAGACGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014526.1
CDS	557..2389
Translation	MQSSTDAGNGGPRKHTQSTAPQKQSEISETTSFTVNANTTTNSQQQQIPGGPASNTPSKNPFKQQLDSNQQNGNMEPDCGIMGVGKEPPVPPPPTYQSQTYAGRQAPPPADQYREEDPRAAGSWAAAKSWMVSWRGSNRLVLVIVAIALLLDNMLLTTVVPIIPEFLYDIRHPDAPLDSFPRTPLTSNVPPPPTPCPCNKDGSEAAPLEISTISPEENETYYRELEERHNELVGETVEVGLLFASKAFVQLLVNPIVGPLTHRIGYSIPMFAGFVIMFLSTIIFAFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRFTDDKERGNAMGIALGGLALGVLIGPPFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKAETEPPSLKSLISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIGRWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPLLTLLKNPPTSDEKKSLIYGRDRAQVRYVTYQNYEEDE

Target ORF information:

RefSeq Version	NM_001014526.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vesicular monoamine transporter, transcript variant E (Vmat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014526.2

ORF Insert Sequence:

ATGCAATCAT CGACCGATGC GGGCAATGGT GGACCAAGAA AACACACGCA GAGCACAGCC 
CCCCAGAAGC AGTCGGAGAT TAGCGAGACC ACCTCCTTCA CCGTAAACGC AAACACCACC 
ACCAACAGCC AGCAGCAACA GATTCCCGGC GGACCCGCCA GCAATACGCC CAGCAAAAAT 
CCCTTCAAGC AGCAGTTGGA CAGCAATCAA CAGAATGGCA ATATGGAGCC GGATTGCGGC 
ATCATGGGCG TCGGCAAGGA GCCACCGGTA CCGCCGCCGC CCACCTACCA GTCGCAGACC 
TACGCAGGAC GACAGGCTCC GCCGCCAGCG GATCAGTACC GCGAGGAGGA TCCACGCGCT 
GCCGGGTCCT GGGCCGCCGC CAAATCCTGG ATGGTCAGCT GGCGGGGATC CAATCGCCTG 
GTGCTCGTCA TCGTGGCCAT AGCCCTGCTC CTCGACAACA TGCTGCTGAC CACAGTGGTG 
CCCATCATAC CCGAGTTCCT GTACGACATA CGACATCCGG ATGCGCCGCT CGACAGCTTT 
CCACGCACAC CTTTAACGTC CAACGTTCCG CCGCCACCGA CGCCGTGTCC ATGCAACAAG 
GATGGCAGCG AGGCGGCTCC GCTCGAGATC TCCACGATTA GTCCGGAGGA GAACGAGACC 
TATTACCGGG AACTGGAGGA GCGGCACAAC GAGCTGGTGG GCGAGACCGT CGAGGTGGGA 
CTTCTATTTG CCTCCAAGGC GTTTGTCCAG CTGCTGGTTA ACCCAATTGT TGGACCCCTG 
ACTCACAGGA TTGGCTATAG TATTCCCATG TTCGCTGGCT TTGTGATAAT GTTCCTCTCA 
ACGATAATTT TCGCTTTTGG ACGATCTTAC CTGGTACTGT TCGTGGCGAG GGCACTGCAG 
GGCATTGGAT CATCCTGCTC CTCGGTTTCC GGAATGGGCA TGCTGGCAGA TCGATTTACG 
GACGACAAGG AGCGTGGAAA TGCCATGGGC ATAGCACTGG GAGGATTGGC TTTGGGCGTG 
CTCATCGGTC CACCATTCGG TGGCGTGATG TACGAGTTTG TGGGCAAGTC GGCTCCATTC 
CTGATCCTGG CTGCCCTGGC CTTGGGCGAT GGCCTGCTAC AGTTGTTCAT GCTGCAGCCA 
TCGATCCAGA AGGCCGAAAC GGAACCGCCC TCGCTGAAGA GCCTCATCAG TGATCCCTAC 
ATCCTGATTG CTGCTGGTGC CATCACGTTC GCCAATATGG GCATTGCCAT GCTGGAACCA 
TCGCTACCGC TGTGGATGGT GGACAACATG GGCGCCACTC GTTGGGAGCA GGGCGTGGCC 
TTCCTGCCCG CCTCCATTAG CTACCTGATT GGCACCAATC TCTTCGGACC GCTGGGACAC 
AAAATTGGAC GATGGTTTGC CGCGTGCTTG GGATTGATTA TCATCGGGGG ATGCTTGATA 
TTTATACCCA TGGCCACTTC GATCACTCAC CTGATCATCC CGAATGCAGG ACTTGGCTTT 
GCCATTGGAA TGGTGGACTC GTCGATGATG CCGGAACTGG GCTATCTGGT GGACATCCGA 
CACTCGGCGG TCTACGGCAG CGTTTACGCC CTGGGTGATG TGGCCTTCTG TGTTGGCTTC 
GCCGTGGGTC CCGCCTTATC TGGATCGCTG GTGAAGAGCA TCGGCTTCGA GTGGATGTTG 
TTTGGAATTG CCATACTCTG CTTTATGTAT GCTCCATTGC TGACGCTGCT CAAGAATCCG 
CCGACCAGCG ACGAGAAGAA GTCTTTGATC TATGGACGCG ATCGGGCTCA GGTACGTTAT 
GTAACCTATC AAAACTACGA GGAAGACGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vmat ( NM_001014525.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014525.1 Drosophila melanogaster vesicular monoamine transporter, transcript variant D (Vmat), mRNA.

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CloneID	OFa11810
Clone ID Related Accession (Same CDS sequence)	NM_001014525.3
Accession Version	NM_001014525.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vesicular monoamine transporter, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001014525.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCAT CGACCGATGC GGGCAATGGT GGACCAAGAA AACACACGCA GAGCACAGCC 
CCCCAGAAGC AGTCGGAGAT TAGCGAGACC ACCTCCTTCA CCGTAAACGC AAACACCACC 
ACCAACAGCC AGCAGCAACA GATTCCCGGC GGACCCGCCA GCAATACGCC CAGCAAAAAT 
CCCTTCAAGC AGCAGTTGGA CAGCAATCAA CAGAATGGCA ATATGGAGCC GGATTGCGGC 
ATCATGGGCG TCGGCAAGGA GCCACCGGTA CCGCCGCCGC CCACCTACCA GTCGCAGACC 
TACGCAGGAC GACAGGCTCC GCCGCCAGCG GATCAGTACC GCGAGGAGGA TCCACGCGCT 
GCCGGGTCCT GGGCCGCCGC CAAATCCTGG ATGGTCAGCT GGCGGGGATC CAATCGCCTG 
GTGCTCGTCA TCGTGGCCAT AGCCCTGCTC CTCGACAACA TGCTGCTGAC CACAGTGGTG 
CCCATCATAC CCGAGTTCCT GTACGACATA CGACATCCGG ATGCGCCGCT CGACAGCTTT 
CCACGCACAC CTTTAACGTC CAACGTTCCG CCGCCACCGA CGCCGTGTCC ATGCAACAAG 
GATGGCAGCG AGGCGGCTCC GCTCGAGATC TCCACGATTA GTCCGGAGGA GAACGAGACC 
TATTACCGGG AACTGGAGGA GCGGCACAAC GAGCTGGTGG GCGAGACCGT CGAGGTGGGA 
CTTCTATTTG CCTCCAAGGC GTTTGTCCAG CTGCTGGTTA ACCCAATTGT TGGACCCCTG 
ACTCACAGGA TTGGCTATAG TATTCCCATG TTCGCTGGCT TTGTGATAAT GTTCCTCTCA 
ACGATAATTT TCGCTTTTGG ACGATCTTAC CTGGTACTGT TCGTGGCGAG GGCACTGCAG 
GGCATTGGAT CATCCTGCTC CTCGGTTTCC GGAATGGGCA TGCTGGCAGA TCGATTTACG 
GACGACAAGG AGCGTGGAAA TGCCATGGGC ATAGCACTGG GAGGATTGGC TTTGGGCGTG 
CTCATCGGTC CACCATTCGG TGGCGTGATG TACGAGTTTG TGGGCAAGTC GGCTCCATTC 
CTGATCCTGG CTGCCCTGGC CTTGGGCGAT GGCCTGCTAC AGTTGTTCAT GCTGCAGCCA 
TCGATCCAGA AGGCCGAAAC GGAACCGCCC TCGCTGAAGA GCCTCATCAG TGATCCCTAC 
ATCCTGATTG CTGCTGGTGC CATCACGTTC GCCAATATGG GCATTGCCAT GCTGGAACCA 
TCGCTACCGC TGTGGATGGT GGACAACATG GGCGCCACTC GTTGGGAGCA GGGCGTGGCC 
TTCCTGCCCG CCTCCATTAG CTACCTGATT GGCACCAATC TCTTCGGACC GCTGGGACAC 
AAAATTGGAC GATGGTTTGC CGCGTGCTTG GGATTGATTA TCATCGGGGG ATGCTTGATA 
TTTATACCCA TGGCCACTTC GATCACTCAC CTGATCATCC CGAATGCAGG ACTTGGCTTT 
GCCATTGGAA TGGTGGACTC GTCGATGATG CCGGAACTGG GCTATCTGGT GGACATCCGA 
CACTCGGCGG TCTACGGCAG CGTTTACGCC CTGGGTGATG TGGCCTTCTG TGTTGGCTTC 
GCCGTGGGTC CCGCCTTATC TGGATCGCTG GTGAAGAGCA TCGGCTTCGA GTGGATGTTG 
TTTGGAATTG CCATACTCTG CTTTATGTAT GCTCCATTGC TGACGCTGCT CAAGAATCCG 
CCGACCAGCG ACGAGAAGAA GGTACGTATG GCCAGAGAGG CGGCTGAGGC TGCTGCAGCC 
GCTGCTGCCG CTTCAGGGGA ATTCTCTGGA ATACCCCAGA TGACCGCATC CTGCCCGGCC 
ATCATGCATG GTGCATCTGT TCCCGATTCG GATGCCGAGG CGGGCAGGAC GAACGAGGCG 
TACGAGAGCG AGAGGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014525.1
CDS	387..2327
Translation	MQSSTDAGNGGPRKHTQSTAPQKQSEISETTSFTVNANTTTNSQQQQIPGGPASNTPSKNPFKQQLDSNQQNGNMEPDCGIMGVGKEPPVPPPPTYQSQTYAGRQAPPPADQYREEDPRAAGSWAAAKSWMVSWRGSNRLVLVIVAIALLLDNMLLTTVVPIIPEFLYDIRHPDAPLDSFPRTPLTSNVPPPPTPCPCNKDGSEAAPLEISTISPEENETYYRELEERHNELVGETVEVGLLFASKAFVQLLVNPIVGPLTHRIGYSIPMFAGFVIMFLSTIIFAFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRFTDDKERGNAMGIALGGLALGVLIGPPFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKAETEPPSLKSLISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIGRWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPLLTLLKNPPTSDEKKVRMAREAAEAAAAAAAASGEFSGIPQMTASCPAIMHGASVPDSDAEAGRTNEAYESERL

Target ORF information:

RefSeq Version	NM_001014525.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vesicular monoamine transporter, transcript variant D (Vmat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014525.3

ORF Insert Sequence:

ATGCAATCAT CGACCGATGC GGGCAATGGT GGACCAAGAA AACACACGCA GAGCACAGCC 
CCCCAGAAGC AGTCGGAGAT TAGCGAGACC ACCTCCTTCA CCGTAAACGC AAACACCACC 
ACCAACAGCC AGCAGCAACA GATTCCCGGC GGACCCGCCA GCAATACGCC CAGCAAAAAT 
CCCTTCAAGC AGCAGTTGGA CAGCAATCAA CAGAATGGCA ATATGGAGCC GGATTGCGGC 
ATCATGGGCG TCGGCAAGGA GCCACCGGTA CCGCCGCCGC CCACCTACCA GTCGCAGACC 
TACGCAGGAC GACAGGCTCC GCCGCCAGCG GATCAGTACC GCGAGGAGGA TCCACGCGCT 
GCCGGGTCCT GGGCCGCCGC CAAATCCTGG ATGGTCAGCT GGCGGGGATC CAATCGCCTG 
GTGCTCGTCA TCGTGGCCAT AGCCCTGCTC CTCGACAACA TGCTGCTGAC CACAGTGGTG 
CCCATCATAC CCGAGTTCCT GTACGACATA CGACATCCGG ATGCGCCGCT CGACAGCTTT 
CCACGCACAC CTTTAACGTC CAACGTTCCG CCGCCACCGA CGCCGTGTCC ATGCAACAAG 
GATGGCAGCG AGGCGGCTCC GCTCGAGATC TCCACGATTA GTCCGGAGGA GAACGAGACC 
TATTACCGGG AACTGGAGGA GCGGCACAAC GAGCTGGTGG GCGAGACCGT CGAGGTGGGA 
CTTCTATTTG CCTCCAAGGC GTTTGTCCAG CTGCTGGTTA ACCCAATTGT TGGACCCCTG 
ACTCACAGGA TTGGCTATAG TATTCCCATG TTCGCTGGCT TTGTGATAAT GTTCCTCTCA 
ACGATAATTT TCGCTTTTGG ACGATCTTAC CTGGTACTGT TCGTGGCGAG GGCACTGCAG 
GGCATTGGAT CATCCTGCTC CTCGGTTTCC GGAATGGGCA TGCTGGCAGA TCGATTTACG 
GACGACAAGG AGCGTGGAAA TGCCATGGGC ATAGCACTGG GAGGATTGGC TTTGGGCGTG 
CTCATCGGTC CACCATTCGG TGGCGTGATG TACGAGTTTG TGGGCAAGTC GGCTCCATTC 
CTGATCCTGG CTGCCCTGGC CTTGGGCGAT GGCCTGCTAC AGTTGTTCAT GCTGCAGCCA 
TCGATCCAGA AGGCCGAAAC GGAACCGCCC TCGCTGAAGA GCCTCATCAG TGATCCCTAC 
ATCCTGATTG CTGCTGGTGC CATCACGTTC GCCAATATGG GCATTGCCAT GCTGGAACCA 
TCGCTACCGC TGTGGATGGT GGACAACATG GGCGCCACTC GTTGGGAGCA GGGCGTGGCC 
TTCCTGCCCG CCTCCATTAG CTACCTGATT GGCACCAATC TCTTCGGACC GCTGGGACAC 
AAAATTGGAC GATGGTTTGC CGCGTGCTTG GGATTGATTA TCATCGGGGG ATGCTTGATA 
TTTATACCCA TGGCCACTTC GATCACTCAC CTGATCATCC CGAATGCAGG ACTTGGCTTT 
GCCATTGGAA TGGTGGACTC GTCGATGATG CCGGAACTGG GCTATCTGGT GGACATCCGA 
CACTCGGCGG TCTACGGCAG CGTTTACGCC CTGGGTGATG TGGCCTTCTG TGTTGGCTTC 
GCCGTGGGTC CCGCCTTATC TGGATCGCTG GTGAAGAGCA TCGGCTTCGA GTGGATGTTG 
TTTGGAATTG CCATACTCTG CTTTATGTAT GCTCCATTGC TGACGCTGCT CAAGAATCCG 
CCGACCAGCG ACGAGAAGAA GGTACGTATG GCCAGAGAGG CGGCTGAGGC TGCTGCAGCC 
GCTGCTGCCG CTTCAGGGGA ATTCTCTGGA ATACCCCAGA TGACCGCATC CTGCCCGGCC 
ATCATGCATG GTGCATCTGT TCCCGATTCG GATGCCGAGG CGGGCAGGAC GAACGAGGCG 
TACGAGAGCG AGAGGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vmat cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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