CG32547 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG32547
Entrez Gene ID	3346150
Full Name	CG32547 gene product from transcript CG32547-RD
Synonyms	CG12610,CG15049,CG15050,Dmel\CG32547
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 17B1-17B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272753.2	NP_001259682.1	uncharacterized protein, isoform D
NM_167622.3	NP_728174.2	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG32547 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG32547 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02566	NM_001272753.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG32547), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa02566	NM_167622.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32547), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG32547 ( NM_001272753.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259682.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG32547), mRNA.

Price & Availability↑

CloneID	OFa02566
Clone ID Related Accession (Same CDS sequence)	NM_001272753.2 , NM_167622.3
Accession Version	NM_001272753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272753.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCCAG CTGAGCAACT TCGCTTGGTC GGAGTATCGG ATGCCTACCA GTTGGCGGCA 
AGCAGTGGAG GAGCAGGAGG AGGAGGAGGA GGAGGAGGCG GCGGCGGCGG CGGCGGAGGA 
CTGGGAGGAT ATGGAGGCGG TGGATCCGGA GGAGATGCCG GCGGCAGTGG CGAGAAAATG 
AAGGATGTGC CCGACAAATA TGTGACGGCA CTCTCGCACT TCCTCGACTG GCATTCCAAT 
GGCACGGTTG ACATGGAGCG ACTTAGTGGT CCCATCCTAA AGTCCAGCAT CAAGTCGGTC 
TATTGGCTGT TTCTCATCCA GTACGCAGCT CTCGCCCTAC TGGGCGTGGT GCTCAATGTG 
ATCATCGTGG TGTACATAAT GTACCATCGA CTTTACAAGG ATGTGACCCA TGCGTTCATC 
ATCAATCTGG CCCTATGCCA TTTTGTCCAG TGCGCCCTCG TCCTGCCCGT CTCGCTGATG 
GTCATGCTCA TCCAGAACTG GATCTTTGGC CAGTTTCTGT GCTTCTTTCT GCCCATGCTG 
CAGGACATAC CGCTGCACGT GGCCATGATC TCCCACATCC TGATCGCCTG GGACCGGATG 
CGCTGGCTGA ACGATCCGCT GAAGGGGCGC CTGCCAGGAT TCGTCTGCTG CTGCGCCACC 
TGGCTAACCG GAATGGTTAT TGCCCTGCCC TACCCCATCT ACACCATCTA CGTGGAGCTA 
GGGGACTACA TGCCGCAGCT GTCGGGGATC GGCCTGTGCG TGGTCAATCT GATGGACGAC 
ATGCAGGAGT ACACGCGCGG ACTCTTCCTG CTGATGTACT GCGGTCCGGC CATCCTGCTG 
TCCTACCTGT ACATCCGCAC CTCCCAGGAG CTGCGTCCGC CCGACGGACC CTTCGCCGTC 
ATGATGTACG AGCATCGCGC GGATCTGCGG ATGCGCCAGA GGAACTCCTC CACATCCTCG 
GTGGAGCCGC GCCACCTGAG CGGCGGGGGT GTGGCAGGAT TGAGCAATGG AGGCGGCAAC 
TCCGCCCGAT CCTACGACTT GTACAGCGCC GAATTGGACG TGCATCGCGA GAAGCGGAAG 
CAGCGCAACT TTGGCAGCAT GGCCGCCACC CAGGTGGTGT GCATGTGCCC ACTGATGATA 
CTGCGCTTCG CCAGGCTCTC GCTGGAGGAG ACCTACGAGA ATGCCAAGCA TTTCGACTTC 
ACCTACCTGA TGTTCGTGTG GGTGGCCTTT CTGCCCACGG TCATCTTTCC CTGCATCTAC 
GCCTCGCAGA TATTGCCCAG AGATGAACAG GAGCGTTTGA GGGGCTATTT TCGTTTATCC 
TCGAAGCGCA AGCAGAAGTC CCAACGTCGC AGCGATGCTG GCGGAGGATC CGGTGTGGGG 
GGCAGTTCCC GCGAGGATTC CATCGAGAAG GACGAGGCCT CGAACACGAC CAGTGTTCAC 
CACGCCGCTG AACCGCACAA GCATTCGTCA AAGTTGCGGC ACGAGCGTGA GGTGCGTCTG 
CCAGGACACG GCTCGTCGGC TGGCGGTGAT CGCTACACAG GAGCACCATA TCGCCAGGGC 
AAGGACAGGG AAAGGGAAAG GGAAAGGGAC AGGGATAGGG AGCGCGATAG GGATAGAGAG 
AGGGAGCGGG AACGGCAGAG GGCGGGTGGT CGCGGTGCCG GAGATGCCGG CGTGGTCAAC 
AATCTGGGCA AAGATGTGCG CGGCAAGAAG CACGACGTCA AGATCAACAT CATCTCAGAT 
GGCGTCGGTC ATGGCGCCAC CAGTCACTCC CATGCCGCTC ATCCCGCCCA TCGCAAACAG 
ACGGGCGGCA ACAACAATAA CAACAACAAC AGCAGTCGAA GTCGCACCAA TCCCGGCCAG 
CGGCATGGTG GCAGCAAGCT AACCGCCGAC TGTCTGTCCA ACGTGACCGC CTCGACGTTC 
TGCAACGGCA GCGGAAGCGT AACTGCCGGC GCAACCGGAA ACGGAAATGG CCCGAGCGGC 
GTACTTATCC CAGACGACAG CAGCACAAGC AACTATGGCG ACGGCGAGGA GAGCTCCGTG 
GTCAGCAGCA TGATGGTGCC GCCGCAACGC TGGCCAGGAT CGGGCGGCGG TGTGCTGCGC 
CACAAGGACG TTTCATTTAG CGAGTGCAGC AGCACCTTTT CGTCGAGCAC TCTGGAGCGC 
GACCTAGAGA TTATGGACCA GCTGGAGCGG GAGCGGAGCA TGGACATCCA AGAGATGCTG 
CAGCGCGAAC GGGAGCGGGA AAAGGTGCGT CGCCAGCTGC CGGACATCGA GAAGCTATAC 
GCGCAGCGTT CGCCGAAGGG AAAGCGCGGC GAGGCAGCTG GAGCCGGAGC CGGAACCGGA 
GCGGGACTGG CGCTGGTCAT GCCCGGCAGT GATCCGCTGA GCAGTCTCTC GCAATCCCGC 
TCGATCTCCA CGGAGTACAG CCTGTGCTCC ACCTTGGAGA CCAGCGGCGC TGGTGTGTCC 
GTTGGCCACG ACGAGGTGCT GCCGCACCAC CTGGAGGAGG TGGAGGAGGA GGTGGTGCCG 
CCGGATTTCT ATGACAGCTA TACGCCAGGC GGCACCCAGA ACCTGCCACC CAATGCCGGT 
GGCGCTGTGC CGGTGGCCAC TGCTGCAGTT CCCGCCTATC AGTACCAGTA CAGTCGCAAT 
CGGCTGAGCC GCAAGAGTTC CGGCTCCAGT TCGGGCCACC ATCATCACGG TCATCATCAT 
GGCCAGCATG GCGGCGGGGG CGTTGGAGGC GTGGCCGGAG GACGCGGCTC CAAGCGGGAC 
AGCTTCAATT CGCTAAACGG CACGGATCTG ATTGCCGGCG CCTTCTGCGA GCTGGATCCC 
ACGCAACCGC TGAACAAGAT GGCCGTGATC GAGGGCCGGA TGCGACGCAG CAGTCCGCGC 
AACGCCAGCT TTAGCTCCGG ATCCGGGGTG TCCGGACGCA GCTCGATGAA GTCCTCGTCG 
CGGGACTATG ACTACGGGCA CGGCTCCTCC TCGCACTCGG CGCATCCGGA GCTCGATTTC 
AGGGAGAACA TCTTTGCGGA GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259682.1
CDS	333..3359
Translation	MSPAEQLRLVGVSDAYQLAASSGGAGGGGGGGGGGGGGGGLGGYGGGGSGGDAGGSGEKMKDVPDKYVTALSHFLDWHSNGTVDMERLSGPILKSSIKSVYWLFLIQYAALALLGVVLNVIIVVYIMYHRLYKDVTHAFIINLALCHFVQCALVLPVSLMVMLIQNWIFGQFLCFFLPMLQDIPLHVAMISHILIAWDRMRWLNDPLKGRLPGFVCCCATWLTGMVIALPYPIYTIYVELGDYMPQLSGIGLCVVNLMDDMQEYTRGLFLLMYCGPAILLSYLYIRTSQELRPPDGPFAVMMYEHRADLRMRQRNSSTSSVEPRHLSGGGVAGLSNGGGNSARSYDLYSAELDVHREKRKQRNFGSMAATQVVCMCPLMILRFARLSLEETYENAKHFDFTYLMFVWVAFLPTVIFPCIYASQILPRDEQERLRGYFRLSSKRKQKSQRRSDAGGGSGVGGSSREDSIEKDEASNTTSVHHAAEPHKHSSKLRHEREVRLPGHGSSAGGDRYTGAPYRQGKDRERERERDRDRERDRDRERERERQRAGGRGAGDAGVVNNLGKDVRGKKHDVKINIISDGVGHGATSHSHAAHPAHRKQTGGNNNNNNNSSRSRTNPGQRHGGSKLTADCLSNVTASTFCNGSGSVTAGATGNGNGPSGVLIPDDSSTSNYGDGEESSVVSSMMVPPQRWPGSGGGVLRHKDVSFSECSSTFSSSTLERDLEIMDQLERERSMDIQEMLQREREREKVRRQLPDIEKLYAQRSPKGKRGEAAGAGAGTGAGLALVMPGSDPLSSLSQSRSISTEYSLCSTLETSGAGVSVGHDEVLPHHLEEVEEEVVPPDFYDSYTPGGTQNLPPNAGGAVPVATAAVPAYQYQYSRNRLSRKSSGSSSGHHHHGHHHGQHGGGGVGGVAGGRGSKRDSFNSLNGTDLIAGAFCELDPTQPLNKMAVIEGRMRRSSPRNASFSSGSGVSGRSSMKSSSRDYDYGHGSSSHSAHPELDFRENIFAEL

Target ORF information:

RefSeq Version	NM_001272753.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG32547), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272753.2

ORF Insert Sequence:

ATGTCGCCAG CTGAGCAACT TCGCTTGGTC GGAGTATCGG ATGCCTACCA GTTGGCGGCA 
AGCAGTGGAG GAGCAGGAGG AGGAGGAGGA GGAGGAGGCG GCGGCGGCGG CGGCGGAGGA 
CTGGGAGGAT ATGGAGGCGG TGGATCCGGA GGAGATGCCG GCGGCAGTGG CGAGAAAATG 
AAGGATGTGC CCGACAAATA TGTGACGGCA CTCTCGCACT TCCTCGACTG GCATTCCAAT 
GGCACGGTTG ACATGGAGCG ACTTAGTGGT CCCATCCTAA AGTCCAGCAT CAAGTCGGTC 
TATTGGCTGT TTCTCATCCA GTACGCAGCT CTCGCCCTAC TGGGCGTGGT GCTCAATGTG 
ATCATCGTGG TGTACATAAT GTACCATCGA CTTTACAAGG ATGTGACCCA TGCGTTCATC 
ATCAATCTGG CCCTATGCCA TTTTGTCCAG TGCGCCCTCG TCCTGCCCGT CTCGCTGATG 
GTCATGCTCA TCCAGAACTG GATCTTTGGC CAGTTTCTGT GCTTCTTTCT GCCCATGCTG 
CAGGACATAC CGCTGCACGT GGCCATGATC TCCCACATCC TGATCGCCTG GGACCGGATG 
CGCTGGCTGA ACGATCCGCT GAAGGGGCGC CTGCCAGGAT TCGTCTGCTG CTGCGCCACC 
TGGCTAACCG GAATGGTTAT TGCCCTGCCC TACCCCATCT ACACCATCTA CGTGGAGCTA 
GGGGACTACA TGCCGCAGCT GTCGGGGATC GGCCTGTGCG TGGTCAATCT GATGGACGAC 
ATGCAGGAGT ACACGCGCGG ACTCTTCCTG CTGATGTACT GCGGTCCGGC CATCCTGCTG 
TCCTACCTGT ACATCCGCAC CTCCCAGGAG CTGCGTCCGC CCGACGGACC CTTCGCCGTC 
ATGATGTACG AGCATCGCGC GGATCTGCGG ATGCGCCAGA GGAACTCCTC CACATCCTCG 
GTGGAGCCGC GCCACCTGAG CGGCGGGGGT GTGGCAGGAT TGAGCAATGG AGGCGGCAAC 
TCCGCCCGAT CCTACGACTT GTACAGCGCC GAATTGGACG TGCATCGCGA GAAGCGGAAG 
CAGCGCAACT TTGGCAGCAT GGCCGCCACC CAGGTGGTGT GCATGTGCCC ACTGATGATA 
CTGCGCTTCG CCAGGCTCTC GCTGGAGGAG ACCTACGAGA ATGCCAAGCA TTTCGACTTC 
ACCTACCTGA TGTTCGTGTG GGTGGCCTTT CTGCCCACGG TCATCTTTCC CTGCATCTAC 
GCCTCGCAGA TATTGCCCAG AGATGAACAG GAGCGTTTGA GGGGCTATTT TCGTTTATCC 
TCGAAGCGCA AGCAGAAGTC CCAACGTCGC AGCGATGCTG GCGGAGGATC CGGTGTGGGG 
GGCAGTTCCC GCGAGGATTC CATCGAGAAG GACGAGGCCT CGAACACGAC CAGTGTTCAC 
CACGCCGCTG AACCGCACAA GCATTCGTCA AAGTTGCGGC ACGAGCGTGA GGTGCGTCTG 
CCAGGACACG GCTCGTCGGC TGGCGGTGAT CGCTACACAG GAGCACCATA TCGCCAGGGC 
AAGGACAGGG AAAGGGAAAG GGAAAGGGAC AGGGATAGGG AGCGCGATAG GGATAGAGAG 
AGGGAGCGGG AACGGCAGAG GGCGGGTGGT CGCGGTGCCG GAGATGCCGG CGTGGTCAAC 
AATCTGGGCA AAGATGTGCG CGGCAAGAAG CACGACGTCA AGATCAACAT CATCTCAGAT 
GGCGTCGGTC ATGGCGCCAC CAGTCACTCC CATGCCGCTC ATCCCGCCCA TCGCAAACAG 
ACGGGCGGCA ACAACAATAA CAACAACAAC AGCAGTCGAA GTCGCACCAA TCCCGGCCAG 
CGGCATGGTG GCAGCAAGCT AACCGCCGAC TGTCTGTCCA ACGTGACCGC CTCGACGTTC 
TGCAACGGCA GCGGAAGCGT AACTGCCGGC GCAACCGGAA ACGGAAATGG CCCGAGCGGC 
GTACTTATCC CAGACGACAG CAGCACAAGC AACTATGGCG ACGGCGAGGA GAGCTCCGTG 
GTCAGCAGCA TGATGGTGCC GCCGCAACGC TGGCCAGGAT CGGGCGGCGG TGTGCTGCGC 
CACAAGGACG TTTCATTTAG CGAGTGCAGC AGCACCTTTT CGTCGAGCAC TCTGGAGCGC 
GACCTAGAGA TTATGGACCA GCTGGAGCGG GAGCGGAGCA TGGACATCCA AGAGATGCTG 
CAGCGCGAAC GGGAGCGGGA AAAGGTGCGT CGCCAGCTGC CGGACATCGA GAAGCTATAC 
GCGCAGCGTT CGCCGAAGGG AAAGCGCGGC GAGGCAGCTG GAGCCGGAGC CGGAACCGGA 
GCGGGACTGG CGCTGGTCAT GCCCGGCAGT GATCCGCTGA GCAGTCTCTC GCAATCCCGC 
TCGATCTCCA CGGAGTACAG CCTGTGCTCC ACCTTGGAGA CCAGCGGCGC TGGTGTGTCC 
GTTGGCCACG ACGAGGTGCT GCCGCACCAC CTGGAGGAGG TGGAGGAGGA GGTGGTGCCG 
CCGGATTTCT ATGACAGCTA TACGCCAGGC GGCACCCAGA ACCTGCCACC CAATGCCGGT 
GGCGCTGTGC CGGTGGCCAC TGCTGCAGTT CCCGCCTATC AGTACCAGTA CAGTCGCAAT 
CGGCTGAGCC GCAAGAGTTC CGGCTCCAGT TCGGGCCACC ATCATCACGG TCATCATCAT 
GGCCAGCATG GCGGCGGGGG CGTTGGAGGC GTGGCCGGAG GACGCGGCTC CAAGCGGGAC 
AGCTTCAATT CGCTAAACGG CACGGATCTG ATTGCCGGCG CCTTCTGCGA GCTGGATCCC 
ACGCAACCGC TGAACAAGAT GGCCGTGATC GAGGGCCGGA TGCGACGCAG CAGTCCGCGC 
AACGCCAGCT TTAGCTCCGG ATCCGGGGTG TCCGGACGCA GCTCGATGAA GTCCTCGTCG 
CGGGACTATG ACTACGGGCA CGGCTCCTCC TCGCACTCGG CGCATCCGGA GCTCGATTTC 
AGGGAGAACA TCTTTGCGGA GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32547 ( NM_167622.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728174.2 Drosophila melanogaster uncharacterized protein, transcript variant C (CG32547), mRNA.

Price & Availability↑

CloneID	OFa02566
Clone ID Related Accession (Same CDS sequence)	NM_001272753.2 , NM_167622.3
Accession Version	NM_167622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167622.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCCAG CTGAGCAACT TCGCTTGGTC GGAGTATCGG ATGCCTACCA GTTGGCGGCA 
AGCAGTGGAG GAGCAGGAGG AGGAGGAGGA GGAGGAGGCG GCGGCGGCGG CGGCGGAGGA 
CTGGGAGGAT ATGGAGGCGG TGGATCCGGA GGAGATGCCG GCGGCAGTGG CGAGAAAATG 
AAGGATGTGC CCGACAAATA TGTGACGGCA CTCTCGCACT TCCTCGACTG GCATTCCAAT 
GGCACGGTTG ACATGGAGCG ACTTAGTGGT CCCATCCTAA AGTCCAGCAT CAAGTCGGTC 
TATTGGCTGT TTCTCATCCA GTACGCAGCT CTCGCCCTAC TGGGCGTGGT GCTCAATGTG 
ATCATCGTGG TGTACATAAT GTACCATCGA CTTTACAAGG ATGTGACCCA TGCGTTCATC 
ATCAATCTGG CCCTATGCCA TTTTGTCCAG TGCGCCCTCG TCCTGCCCGT CTCGCTGATG 
GTCATGCTCA TCCAGAACTG GATCTTTGGC CAGTTTCTGT GCTTCTTTCT GCCCATGCTG 
CAGGACATAC CGCTGCACGT GGCCATGATC TCCCACATCC TGATCGCCTG GGACCGGATG 
CGCTGGCTGA ACGATCCGCT GAAGGGGCGC CTGCCAGGAT TCGTCTGCTG CTGCGCCACC 
TGGCTAACCG GAATGGTTAT TGCCCTGCCC TACCCCATCT ACACCATCTA CGTGGAGCTA 
GGGGACTACA TGCCGCAGCT GTCGGGGATC GGCCTGTGCG TGGTCAATCT GATGGACGAC 
ATGCAGGAGT ACACGCGCGG ACTCTTCCTG CTGATGTACT GCGGTCCGGC CATCCTGCTG 
TCCTACCTGT ACATCCGCAC CTCCCAGGAG CTGCGTCCGC CCGACGGACC CTTCGCCGTC 
ATGATGTACG AGCATCGCGC GGATCTGCGG ATGCGCCAGA GGAACTCCTC CACATCCTCG 
GTGGAGCCGC GCCACCTGAG CGGCGGGGGT GTGGCAGGAT TGAGCAATGG AGGCGGCAAC 
TCCGCCCGAT CCTACGACTT GTACAGCGCC GAATTGGACG TGCATCGCGA GAAGCGGAAG 
CAGCGCAACT TTGGCAGCAT GGCCGCCACC CAGGTGGTGT GCATGTGCCC ACTGATGATA 
CTGCGCTTCG CCAGGCTCTC GCTGGAGGAG ACCTACGAGA ATGCCAAGCA TTTCGACTTC 
ACCTACCTGA TGTTCGTGTG GGTGGCCTTT CTGCCCACGG TCATCTTTCC CTGCATCTAC 
GCCTCGCAGA TATTGCCCAG AGATGAACAG GAGCGTTTGA GGGGCTATTT TCGTTTATCC 
TCGAAGCGCA AGCAGAAGTC CCAACGTCGC AGCGATGCTG GCGGAGGATC CGGTGTGGGG 
GGCAGTTCCC GCGAGGATTC CATCGAGAAG GACGAGGCCT CGAACACGAC CAGTGTTCAC 
CACGCCGCTG AACCGCACAA GCATTCGTCA AAGTTGCGGC ACGAGCGTGA GGTGCGTCTG 
CCAGGACACG GCTCGTCGGC TGGCGGTGAT CGCTACACAG GAGCACCATA TCGCCAGGGC 
AAGGACAGGG AAAGGGAAAG GGAAAGGGAC AGGGATAGGG AGCGCGATAG GGATAGAGAG 
AGGGAGCGGG AACGGCAGAG GGCGGGTGGT CGCGGTGCCG GAGATGCCGG CGTGGTCAAC 
AATCTGGGCA AAGATGTGCG CGGCAAGAAG CACGACGTCA AGATCAACAT CATCTCAGAT 
GGCGTCGGTC ATGGCGCCAC CAGTCACTCC CATGCCGCTC ATCCCGCCCA TCGCAAACAG 
ACGGGCGGCA ACAACAATAA CAACAACAAC AGCAGTCGAA GTCGCACCAA TCCCGGCCAG 
CGGCATGGTG GCAGCAAGCT AACCGCCGAC TGTCTGTCCA ACGTGACCGC CTCGACGTTC 
TGCAACGGCA GCGGAAGCGT AACTGCCGGC GCAACCGGAA ACGGAAATGG CCCGAGCGGC 
GTACTTATCC CAGACGACAG CAGCACAAGC AACTATGGCG ACGGCGAGGA GAGCTCCGTG 
GTCAGCAGCA TGATGGTGCC GCCGCAACGC TGGCCAGGAT CGGGCGGCGG TGTGCTGCGC 
CACAAGGACG TTTCATTTAG CGAGTGCAGC AGCACCTTTT CGTCGAGCAC TCTGGAGCGC 
GACCTAGAGA TTATGGACCA GCTGGAGCGG GAGCGGAGCA TGGACATCCA AGAGATGCTG 
CAGCGCGAAC GGGAGCGGGA AAAGGTGCGT CGCCAGCTGC CGGACATCGA GAAGCTATAC 
GCGCAGCGTT CGCCGAAGGG AAAGCGCGGC GAGGCAGCTG GAGCCGGAGC CGGAACCGGA 
GCGGGACTGG CGCTGGTCAT GCCCGGCAGT GATCCGCTGA GCAGTCTCTC GCAATCCCGC 
TCGATCTCCA CGGAGTACAG CCTGTGCTCC ACCTTGGAGA CCAGCGGCGC TGGTGTGTCC 
GTTGGCCACG ACGAGGTGCT GCCGCACCAC CTGGAGGAGG TGGAGGAGGA GGTGGTGCCG 
CCGGATTTCT ATGACAGCTA TACGCCAGGC GGCACCCAGA ACCTGCCACC CAATGCCGGT 
GGCGCTGTGC CGGTGGCCAC TGCTGCAGTT CCCGCCTATC AGTACCAGTA CAGTCGCAAT 
CGGCTGAGCC GCAAGAGTTC CGGCTCCAGT TCGGGCCACC ATCATCACGG TCATCATCAT 
GGCCAGCATG GCGGCGGGGG CGTTGGAGGC GTGGCCGGAG GACGCGGCTC CAAGCGGGAC 
AGCTTCAATT CGCTAAACGG CACGGATCTG ATTGCCGGCG CCTTCTGCGA GCTGGATCCC 
ACGCAACCGC TGAACAAGAT GGCCGTGATC GAGGGCCGGA TGCGACGCAG CAGTCCGCGC 
AACGCCAGCT TTAGCTCCGG ATCCGGGGTG TCCGGACGCA GCTCGATGAA GTCCTCGTCG 
CGGGACTATG ACTACGGGCA CGGCTCCTCC TCGCACTCGG CGCATCCGGA GCTCGATTTC 
AGGGAGAACA TCTTTGCGGA GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728174.2
CDS	333..3359
Translation	MSPAEQLRLVGVSDAYQLAASSGGAGGGGGGGGGGGGGGGLGGYGGGGSGGDAGGSGEKMKDVPDKYVTALSHFLDWHSNGTVDMERLSGPILKSSIKSVYWLFLIQYAALALLGVVLNVIIVVYIMYHRLYKDVTHAFIINLALCHFVQCALVLPVSLMVMLIQNWIFGQFLCFFLPMLQDIPLHVAMISHILIAWDRMRWLNDPLKGRLPGFVCCCATWLTGMVIALPYPIYTIYVELGDYMPQLSGIGLCVVNLMDDMQEYTRGLFLLMYCGPAILLSYLYIRTSQELRPPDGPFAVMMYEHRADLRMRQRNSSTSSVEPRHLSGGGVAGLSNGGGNSARSYDLYSAELDVHREKRKQRNFGSMAATQVVCMCPLMILRFARLSLEETYENAKHFDFTYLMFVWVAFLPTVIFPCIYASQILPRDEQERLRGYFRLSSKRKQKSQRRSDAGGGSGVGGSSREDSIEKDEASNTTSVHHAAEPHKHSSKLRHEREVRLPGHGSSAGGDRYTGAPYRQGKDRERERERDRDRERDRDRERERERQRAGGRGAGDAGVVNNLGKDVRGKKHDVKINIISDGVGHGATSHSHAAHPAHRKQTGGNNNNNNNSSRSRTNPGQRHGGSKLTADCLSNVTASTFCNGSGSVTAGATGNGNGPSGVLIPDDSSTSNYGDGEESSVVSSMMVPPQRWPGSGGGVLRHKDVSFSECSSTFSSSTLERDLEIMDQLERERSMDIQEMLQREREREKVRRQLPDIEKLYAQRSPKGKRGEAAGAGAGTGAGLALVMPGSDPLSSLSQSRSISTEYSLCSTLETSGAGVSVGHDEVLPHHLEEVEEEVVPPDFYDSYTPGGTQNLPPNAGGAVPVATAAVPAYQYQYSRNRLSRKSSGSSSGHHHHGHHHGQHGGGGVGGVAGGRGSKRDSFNSLNGTDLIAGAFCELDPTQPLNKMAVIEGRMRRSSPRNASFSSGSGVSGRSSMKSSSRDYDYGHGSSSHSAHPELDFRENIFAEL

Target ORF information:

RefSeq Version	NM_167622.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32547), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167622.3

ORF Insert Sequence:

ATGTCGCCAG CTGAGCAACT TCGCTTGGTC GGAGTATCGG ATGCCTACCA GTTGGCGGCA 
AGCAGTGGAG GAGCAGGAGG AGGAGGAGGA GGAGGAGGCG GCGGCGGCGG CGGCGGAGGA 
CTGGGAGGAT ATGGAGGCGG TGGATCCGGA GGAGATGCCG GCGGCAGTGG CGAGAAAATG 
AAGGATGTGC CCGACAAATA TGTGACGGCA CTCTCGCACT TCCTCGACTG GCATTCCAAT 
GGCACGGTTG ACATGGAGCG ACTTAGTGGT CCCATCCTAA AGTCCAGCAT CAAGTCGGTC 
TATTGGCTGT TTCTCATCCA GTACGCAGCT CTCGCCCTAC TGGGCGTGGT GCTCAATGTG 
ATCATCGTGG TGTACATAAT GTACCATCGA CTTTACAAGG ATGTGACCCA TGCGTTCATC 
ATCAATCTGG CCCTATGCCA TTTTGTCCAG TGCGCCCTCG TCCTGCCCGT CTCGCTGATG 
GTCATGCTCA TCCAGAACTG GATCTTTGGC CAGTTTCTGT GCTTCTTTCT GCCCATGCTG 
CAGGACATAC CGCTGCACGT GGCCATGATC TCCCACATCC TGATCGCCTG GGACCGGATG 
CGCTGGCTGA ACGATCCGCT GAAGGGGCGC CTGCCAGGAT TCGTCTGCTG CTGCGCCACC 
TGGCTAACCG GAATGGTTAT TGCCCTGCCC TACCCCATCT ACACCATCTA CGTGGAGCTA 
GGGGACTACA TGCCGCAGCT GTCGGGGATC GGCCTGTGCG TGGTCAATCT GATGGACGAC 
ATGCAGGAGT ACACGCGCGG ACTCTTCCTG CTGATGTACT GCGGTCCGGC CATCCTGCTG 
TCCTACCTGT ACATCCGCAC CTCCCAGGAG CTGCGTCCGC CCGACGGACC CTTCGCCGTC 
ATGATGTACG AGCATCGCGC GGATCTGCGG ATGCGCCAGA GGAACTCCTC CACATCCTCG 
GTGGAGCCGC GCCACCTGAG CGGCGGGGGT GTGGCAGGAT TGAGCAATGG AGGCGGCAAC 
TCCGCCCGAT CCTACGACTT GTACAGCGCC GAATTGGACG TGCATCGCGA GAAGCGGAAG 
CAGCGCAACT TTGGCAGCAT GGCCGCCACC CAGGTGGTGT GCATGTGCCC ACTGATGATA 
CTGCGCTTCG CCAGGCTCTC GCTGGAGGAG ACCTACGAGA ATGCCAAGCA TTTCGACTTC 
ACCTACCTGA TGTTCGTGTG GGTGGCCTTT CTGCCCACGG TCATCTTTCC CTGCATCTAC 
GCCTCGCAGA TATTGCCCAG AGATGAACAG GAGCGTTTGA GGGGCTATTT TCGTTTATCC 
TCGAAGCGCA AGCAGAAGTC CCAACGTCGC AGCGATGCTG GCGGAGGATC CGGTGTGGGG 
GGCAGTTCCC GCGAGGATTC CATCGAGAAG GACGAGGCCT CGAACACGAC CAGTGTTCAC 
CACGCCGCTG AACCGCACAA GCATTCGTCA AAGTTGCGGC ACGAGCGTGA GGTGCGTCTG 
CCAGGACACG GCTCGTCGGC TGGCGGTGAT CGCTACACAG GAGCACCATA TCGCCAGGGC 
AAGGACAGGG AAAGGGAAAG GGAAAGGGAC AGGGATAGGG AGCGCGATAG GGATAGAGAG 
AGGGAGCGGG AACGGCAGAG GGCGGGTGGT CGCGGTGCCG GAGATGCCGG CGTGGTCAAC 
AATCTGGGCA AAGATGTGCG CGGCAAGAAG CACGACGTCA AGATCAACAT CATCTCAGAT 
GGCGTCGGTC ATGGCGCCAC CAGTCACTCC CATGCCGCTC ATCCCGCCCA TCGCAAACAG 
ACGGGCGGCA ACAACAATAA CAACAACAAC AGCAGTCGAA GTCGCACCAA TCCCGGCCAG 
CGGCATGGTG GCAGCAAGCT AACCGCCGAC TGTCTGTCCA ACGTGACCGC CTCGACGTTC 
TGCAACGGCA GCGGAAGCGT AACTGCCGGC GCAACCGGAA ACGGAAATGG CCCGAGCGGC 
GTACTTATCC CAGACGACAG CAGCACAAGC AACTATGGCG ACGGCGAGGA GAGCTCCGTG 
GTCAGCAGCA TGATGGTGCC GCCGCAACGC TGGCCAGGAT CGGGCGGCGG TGTGCTGCGC 
CACAAGGACG TTTCATTTAG CGAGTGCAGC AGCACCTTTT CGTCGAGCAC TCTGGAGCGC 
GACCTAGAGA TTATGGACCA GCTGGAGCGG GAGCGGAGCA TGGACATCCA AGAGATGCTG 
CAGCGCGAAC GGGAGCGGGA AAAGGTGCGT CGCCAGCTGC CGGACATCGA GAAGCTATAC 
GCGCAGCGTT CGCCGAAGGG AAAGCGCGGC GAGGCAGCTG GAGCCGGAGC CGGAACCGGA 
GCGGGACTGG CGCTGGTCAT GCCCGGCAGT GATCCGCTGA GCAGTCTCTC GCAATCCCGC 
TCGATCTCCA CGGAGTACAG CCTGTGCTCC ACCTTGGAGA CCAGCGGCGC TGGTGTGTCC 
GTTGGCCACG ACGAGGTGCT GCCGCACCAC CTGGAGGAGG TGGAGGAGGA GGTGGTGCCG 
CCGGATTTCT ATGACAGCTA TACGCCAGGC GGCACCCAGA ACCTGCCACC CAATGCCGGT 
GGCGCTGTGC CGGTGGCCAC TGCTGCAGTT CCCGCCTATC AGTACCAGTA CAGTCGCAAT 
CGGCTGAGCC GCAAGAGTTC CGGCTCCAGT TCGGGCCACC ATCATCACGG TCATCATCAT 
GGCCAGCATG GCGGCGGGGG CGTTGGAGGC GTGGCCGGAG GACGCGGCTC CAAGCGGGAC 
AGCTTCAATT CGCTAAACGG CACGGATCTG ATTGCCGGCG CCTTCTGCGA GCTGGATCCC 
ACGCAACCGC TGAACAAGAT GGCCGTGATC GAGGGCCGGA TGCGACGCAG CAGTCCGCGC 
AACGCCAGCT TTAGCTCCGG ATCCGGGGTG TCCGGACGCA GCTCGATGAA GTCCTCGTCG 
CGGGACTATG ACTACGGGCA CGGCTCCTCC TCGCACTCGG CGCATCCGGA GCTCGATTTC 
AGGGAGAACA TCTTTGCGGA GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG32547 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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