insv cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	insv
Entrez Gene ID	33441
Full Name	insensitive
Synonyms	BcDNA:RE55538,CG3227,Dmel\CG3227,Insv
General protein information	Preferred Names insensitive Names CG3227 gene product from transcript CG3227-RA CG3227-PA CG3227-PB insv-PA insv-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23A1-23A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_134846.3	NP_608690.2	insensitive, isoform A
NM_001298637.1	NP_001285566.1	insensitive, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data shed first light on the in vivo function for a BEN-solo protein, Insv, as an Su(H) corepressor in the Notch pathway regulating neural development.
Title: Insensitive is a corepressor for Suppressor of Hairless and regulates Notch signalling during neural development.

The following insv gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the insv cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13513	NM_134846.3 Latest version!	Drosophila melanogaster insensitive (insv), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13513	NM_001298637.1 Latest version!	Drosophila melanogaster insensitive (insv), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

insv ( NM_134846.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608690.2 Drosophila melanogaster insensitive (insv), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13513
Clone ID Related Accession (Same CDS sequence)	NM_134846.3 , NM_001298637.1
Accession Version	NM_134846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	insensitive, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134846.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAACC AATATCAGCG CATAGTCAGC GCCACTGCAC GCGAGGATTC GCAGGCAAGG 
AGGAGCAAGC CCTTGTTTCT GAATGCCTGG TCGCAGACTT CTAGCGTGGA CTTCGAGGTG 
CTTCGCAGCA TCTCCGCGCC GGACCCGCAA GTGGAGGTCG AGAACAGAGC GCTCCGCGAT 
AAGGTCCGCT ACTTGGAGGC CAAACTGCAG CAGCACAAGG ATCTGCTATC CCAGATTCAT 
GCCACCTCCG CCAGGATGCA ACAGGCATCC TCCCTGCTGG CAGAAAGTAG ACCACCCACG 
CCGCCCGCGG TCCAGAGCCA CCACATTCTC ACGCCACCGA GTTCAGAGAT ATGTGCGCCC 
GCGCAGAATC CCCAGATTCT GGACTACAAG ATCATCTCGG CACCCGACGA TGCGGATGCC 
ATTGAGATTC GCCTGGCGGC GGAGAGTCTG AACAGCCTGA GCACCTCTGC GGACTCAGAT 
CGCCTGGAGA TTTGCCTGGG CGACGAGAAT CATCAGCAGA GCAATCATCA CAATTCCCAG 
CAGCAGTACA GGATTAGCAA CGGCATAAAG AGGGATGGTT CCTCCGAGTC CGCCGACACT 
CCACTACAGT TCATCAAACG CCGCAAGCTG CAGGAGATTC AGTTGCAGGA AGAAATCCCG 
CGGCAGAACA TAAAACTACC AGCCAAACCC CAAGTCAAAG CCCGTAATGG CAGTTTTACC 
GATCTCAACA AGGGCCAACA GCAAAACATG GACAACGTGA TGGTGTCCAT TGGCCCGAAC 
AACACCTGTG TGCCCGCCAG TGTCTTCGAG AATATTAACT GGTCGGTGTG CTCGCTGGCC 
ACCCGGAAAC TACTGGTCAC GATCTTCGAC CGGGAAACTC TGGCCACCCA TTCGATGACG 
GGCAAGCCGT CGCCCGCCTT CAAGGATCAG GACAAGCCGC TCAAGCGAAT GCTCGATCCT 
GGGAAGATCC AGGACATCAT ATTCGCAGTG ACGCACAAGT GTAATGCCAG TGAGAAGGAG 
GTGCGCAACG CCATCACCAC CAAGTGCGCC GACGAGAACA AGATGATGAA GATCCAGAAC 
GTCAAGCGAC GCAGCAGTGG CATCAAACAC GAAAAGGAGA ACATTATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608690.2
CDS	353..1483
Translation	MENQYQRIVSATAREDSQARRSKPLFLNAWSQTSSVDFEVLRSISAPDPQVEVENRALRDKVRYLEAKLQQHKDLLSQIHATSARMQQASSLLAESRPPTPPAVQSHHILTPPSSEICAPAQNPQILDYKIISAPDDADAIEIRLAAESLNSLSTSADSDRLEICLGDENHQQSNHHNSQQQYRISNGIKRDGSSESADTPLQFIKRRKLQEIQLQEEIPRQNIKLPAKPQVKARNGSFTDLNKGQQQNMDNVMVSIGPNNTCVPASVFENINWSVCSLATRKLLVTIFDRETLATHSMTGKPSPAFKDQDKPLKRMLDPGKIQDIIFAVTHKCNASEKEVRNAITTKCADENKMMKIQNVKRRSSGIKHEKENII

Target ORF information:

RefSeq Version	NM_134846.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster insensitive (insv), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134846.3

ORF Insert Sequence:

ATGGAAAACC AATATCAGCG CATAGTCAGC GCCACTGCAC GCGAGGATTC GCAGGCAAGG 
AGGAGCAAGC CCTTGTTTCT GAATGCCTGG TCGCAGACTT CTAGCGTGGA CTTCGAGGTG 
CTTCGCAGCA TCTCCGCGCC GGACCCGCAA GTGGAGGTCG AGAACAGAGC GCTCCGCGAT 
AAGGTCCGCT ACTTGGAGGC CAAACTGCAG CAGCACAAGG ATCTGCTATC CCAGATTCAT 
GCCACCTCCG CCAGGATGCA ACAGGCATCC TCCCTGCTGG CAGAAAGTAG ACCACCCACG 
CCGCCCGCGG TCCAGAGCCA CCACATTCTC ACGCCACCGA GTTCAGAGAT ATGTGCGCCC 
GCGCAGAATC CCCAGATTCT GGACTACAAG ATCATCTCGG CACCCGACGA TGCGGATGCC 
ATTGAGATTC GCCTGGCGGC GGAGAGTCTG AACAGCCTGA GCACCTCTGC GGACTCAGAT 
CGCCTGGAGA TTTGCCTGGG CGACGAGAAT CATCAGCAGA GCAATCATCA CAATTCCCAG 
CAGCAGTACA GGATTAGCAA CGGCATAAAG AGGGATGGTT CCTCCGAGTC CGCCGACACT 
CCACTACAGT TCATCAAACG CCGCAAGCTG CAGGAGATTC AGTTGCAGGA AGAAATCCCG 
CGGCAGAACA TAAAACTACC AGCCAAACCC CAAGTCAAAG CCCGTAATGG CAGTTTTACC 
GATCTCAACA AGGGCCAACA GCAAAACATG GACAACGTGA TGGTGTCCAT TGGCCCGAAC 
AACACCTGTG TGCCCGCCAG TGTCTTCGAG AATATTAACT GGTCGGTGTG CTCGCTGGCC 
ACCCGGAAAC TACTGGTCAC GATCTTCGAC CGGGAAACTC TGGCCACCCA TTCGATGACG 
GGCAAGCCGT CGCCCGCCTT CAAGGATCAG GACAAGCCGC TCAAGCGAAT GCTCGATCCT 
GGGAAGATCC AGGACATCAT ATTCGCAGTG ACGCACAAGT GTAATGCCAG TGAGAAGGAG 
GTGCGCAACG CCATCACCAC CAAGTGCGCC GACGAGAACA AGATGATGAA GATCCAGAAC 
GTCAAGCGAC GCAGCAGTGG CATCAAACAC GAAAAGGAGA ACATTATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

insv ( NM_001298637.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285566.1 Drosophila melanogaster insensitive (insv), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13513
Clone ID Related Accession (Same CDS sequence)	NM_134846.3 , NM_001298637.1
Accession Version	NM_001298637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	insensitive, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAACC AATATCAGCG CATAGTCAGC GCCACTGCAC GCGAGGATTC GCAGGCAAGG 
AGGAGCAAGC CCTTGTTTCT GAATGCCTGG TCGCAGACTT CTAGCGTGGA CTTCGAGGTG 
CTTCGCAGCA TCTCCGCGCC GGACCCGCAA GTGGAGGTCG AGAACAGAGC GCTCCGCGAT 
AAGGTCCGCT ACTTGGAGGC CAAACTGCAG CAGCACAAGG ATCTGCTATC CCAGATTCAT 
GCCACCTCCG CCAGGATGCA ACAGGCATCC TCCCTGCTGG CAGAAAGTAG ACCACCCACG 
CCGCCCGCGG TCCAGAGCCA CCACATTCTC ACGCCACCGA GTTCAGAGAT ATGTGCGCCC 
GCGCAGAATC CCCAGATTCT GGACTACAAG ATCATCTCGG CACCCGACGA TGCGGATGCC 
ATTGAGATTC GCCTGGCGGC GGAGAGTCTG AACAGCCTGA GCACCTCTGC GGACTCAGAT 
CGCCTGGAGA TTTGCCTGGG CGACGAGAAT CATCAGCAGA GCAATCATCA CAATTCCCAG 
CAGCAGTACA GGATTAGCAA CGGCATAAAG AGGGATGGTT CCTCCGAGTC CGCCGACACT 
CCACTACAGT TCATCAAACG CCGCAAGCTG CAGGAGATTC AGTTGCAGGA AGAAATCCCG 
CGGCAGAACA TAAAACTACC AGCCAAACCC CAAGTCAAAG CCCGTAATGG CAGTTTTACC 
GATCTCAACA AGGGCCAACA GCAAAACATG GACAACGTGA TGGTGTCCAT TGGCCCGAAC 
AACACCTGTG TGCCCGCCAG TGTCTTCGAG AATATTAACT GGTCGGTGTG CTCGCTGGCC 
ACCCGGAAAC TACTGGTCAC GATCTTCGAC CGGGAAACTC TGGCCACCCA TTCGATGACG 
GGCAAGCCGT CGCCCGCCTT CAAGGATCAG GACAAGCCGC TCAAGCGAAT GCTCGATCCT 
GGGAAGATCC AGGACATCAT ATTCGCAGTG ACGCACAAGT GTAATGCCAG TGAGAAGGAG 
GTGCGCAACG CCATCACCAC CAAGTGCGCC GACGAGAACA AGATGATGAA GATCCAGAAC 
GTCAAGCGAC GCAGCAGTGG CATCAAACAC GAAAAGGAGA ACATTATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285566.1
CDS	34..1164
Translation	MENQYQRIVSATAREDSQARRSKPLFLNAWSQTSSVDFEVLRSISAPDPQVEVENRALRDKVRYLEAKLQQHKDLLSQIHATSARMQQASSLLAESRPPTPPAVQSHHILTPPSSEICAPAQNPQILDYKIISAPDDADAIEIRLAAESLNSLSTSADSDRLEICLGDENHQQSNHHNSQQQYRISNGIKRDGSSESADTPLQFIKRRKLQEIQLQEEIPRQNIKLPAKPQVKARNGSFTDLNKGQQQNMDNVMVSIGPNNTCVPASVFENINWSVCSLATRKLLVTIFDRETLATHSMTGKPSPAFKDQDKPLKRMLDPGKIQDIIFAVTHKCNASEKEVRNAITTKCADENKMMKIQNVKRRSSGIKHEKENII

Target ORF information:

RefSeq Version	NM_001298637.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster insensitive (insv), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298637.1

ORF Insert Sequence:

ATGGAAAACC AATATCAGCG CATAGTCAGC GCCACTGCAC GCGAGGATTC GCAGGCAAGG 
AGGAGCAAGC CCTTGTTTCT GAATGCCTGG TCGCAGACTT CTAGCGTGGA CTTCGAGGTG 
CTTCGCAGCA TCTCCGCGCC GGACCCGCAA GTGGAGGTCG AGAACAGAGC GCTCCGCGAT 
AAGGTCCGCT ACTTGGAGGC CAAACTGCAG CAGCACAAGG ATCTGCTATC CCAGATTCAT 
GCCACCTCCG CCAGGATGCA ACAGGCATCC TCCCTGCTGG CAGAAAGTAG ACCACCCACG 
CCGCCCGCGG TCCAGAGCCA CCACATTCTC ACGCCACCGA GTTCAGAGAT ATGTGCGCCC 
GCGCAGAATC CCCAGATTCT GGACTACAAG ATCATCTCGG CACCCGACGA TGCGGATGCC 
ATTGAGATTC GCCTGGCGGC GGAGAGTCTG AACAGCCTGA GCACCTCTGC GGACTCAGAT 
CGCCTGGAGA TTTGCCTGGG CGACGAGAAT CATCAGCAGA GCAATCATCA CAATTCCCAG 
CAGCAGTACA GGATTAGCAA CGGCATAAAG AGGGATGGTT CCTCCGAGTC CGCCGACACT 
CCACTACAGT TCATCAAACG CCGCAAGCTG CAGGAGATTC AGTTGCAGGA AGAAATCCCG 
CGGCAGAACA TAAAACTACC AGCCAAACCC CAAGTCAAAG CCCGTAATGG CAGTTTTACC 
GATCTCAACA AGGGCCAACA GCAAAACATG GACAACGTGA TGGTGTCCAT TGGCCCGAAC 
AACACCTGTG TGCCCGCCAG TGTCTTCGAG AATATTAACT GGTCGGTGTG CTCGCTGGCC 
ACCCGGAAAC TACTGGTCAC GATCTTCGAC CGGGAAACTC TGGCCACCCA TTCGATGACG 
GGCAAGCCGT CGCCCGCCTT CAAGGATCAG GACAAGCCGC TCAAGCGAAT GCTCGATCCT 
GGGAAGATCC AGGACATCAT ATTCGCAGTG ACGCACAAGT GTAATGCCAG TGAGAAGGAG 
GTGCGCAACG CCATCACCAC CAAGTGCGCC GACGAGAACA AGATGATGAA GATCCAGAAC 
GTCAAGCGAC GCAGCAGTGG CATCAAACAC GAAAAGGAGA ACATTATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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insv cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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