CG5397 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5397
Entrez Gene ID	33313
Full Name	CG5397 gene product from transcript CG5397-RB
Synonyms	CT16527,Dmel\CG5397
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21F3-21F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_134747.3	NP_608591.1	uncharacterized protein, isoform A
NM_001272936.1	NP_001259865.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5397	NP_001259865.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013509	XP_003436330.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5397 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5397 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13447	NM_134747.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5397), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13447	NM_001272936.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5397), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5397 ( NM_134747.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608591.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG5397), mRNA.

Price & Availability↑

CloneID	OFa13447
Clone ID Related Accession (Same CDS sequence)	NM_134747.3 , NM_001272936.1
Accession Version	NM_134747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134747.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCTC AGCTGAAGCG GAACACTTTG TTGCTACTGC TACTTTCGGT GGTGATCTTG 
GCCAGCTGCA AGTCTGGCGG GGATGCGCAC GTTCGCGTGA AGCGGATAGT GGGTGGCAAG 
CAGTCGAAGG CGCCGCCCGT CGATGATCCC GTGATCTTCG CGAGACTCTT TGATCGCGAT 
GCGCGAGTGG AGGGATTCCG GAATCCCAGC ACGGGCATCT ACTCCTTTCT GGGAATGCAC 
TACGCGGAGC CACCTGTTGG TCCACTGAGA TACTCCAGAC CGGTGTACAA AAGGCTGGCT 
GGTGACTTCA ATGCCACCAA ACATGGCCCA CCTTGTATAC AGCCGCATCC GCAGTTTCCT 
CAGAGGATTA TCGGCGATGA GGATTGCCTG CTGCTGAACG TCTACACCCC GCAAATGCCG 
GATGAGACCA CAGGGCTGCC AGTGTTCGTT TGGATTCACC CGGGTGGCTA TCGATATGGA 
TCGGCGGCCC AGTACGATGC CACGCCTATG GCCCAAAGGG GCGCAATCGT GGTGGCTCCT 
CAGTATCGCC TGGGATCACT GGGAATAATG GGGGATGGCA CCAAGCAGTT CGATGGAAAC 
CTGGCCATGT TCGACTTGGC CGCCGCTCTC CGTTGGGTCA CCGATTACAT CTCCTATTTC 
GGGGGCAATC CCAAGCAGGT CCAGGCCATA GGCCACGGAT CCGGAGCAGC CAGTGCCATG 
TATCTATCGA TGTCGCCGAC GTCGCGAAGC GCTGGCGATG TTCACGGAGT GGTGGCCATG 
TCGGGAACCG CTCTCTCTCA GTATGCCATG GACAAGGAAC CCGTTCAGAG CGTCCAGGAA 
GTGGCCAAAA TCAATGGCTG TCCCACAGGA AACGAGCTGG AAATCGTCAA TTGCTTAAGG 
AGTAAAAGTG CTGAGGACAT AATCAAGAAC GATGATAAAG TTCAAACGGA GCGTTTGGCA 
GGACGTGCCC TGGTCAAAGG ACTAACTGGA AACGTGGGCT TCCAGCCTCA CATCGAGAGC 
GAGGACGATG GTAGAGCATT GCCCAGTTTG ATAGTTGGAG AACCGGAGCA GCAGCTCAAG 
AGCAGTAACT TCTCGGGGAT TCCCCTGCTG ACTGGAGTCA CCAAGCACGA GACAGCAAAT 
TCCGTCACTG TGGAAACGAT AGAAAAGGTC TTTGGATCGG CGGAGCAGTT CCTGGGATCA 
CTAAGTGATT CTCTGAACAA GCTGACGAGT TTTCTGAAAA TCGACAAGCT AACCGGTCAG 
ATCGCCAAAC CGGAACTGCC GGGCCTCACG AGTGTCCTGA CTCCCACTCT CCAGGACGTT 
TGGAAGGTGC CGCAGGCGCT GAATGTGGAT CAAGTACTTT CCAAGGTGGT GGAATCAACG 
ACCGACGTGC TGTTCAACCT TCCGGCTGTT CTGACCACCC AGGTTTGGTC CAGGCTAGCT 
CCAGCCTTTA TGTACAGCTT CGAGTACAAT GGCACCAAGT CGAAGGGAAT AAACTTCCTG 
AAGGGTCTAC CAATTGTCTC GGAAACAGCC CATGATAAAC CGGAAACGGT GGGTCATGGT 
GATGAGATCG GCTACATGTT CGATGCAAAT GACATATTCG GGAATCCCAT GGAGGAGACT 
CGTTTGACCA GCGCCGAGGA TCTCAAAGTG CGCAACAACC TCATCGATCT GTTGGTCAAG 
TTTGCCAATA AGGACAAGGA AGAGGGCGGA AAGAGTTCCA TTTTCCAGAG TGTTACGGGA 
AAAGCCACGC CTTTTATTAA GATAGATACC AAACTGCAGA CCTCCAATGA TTTCCGCTTC 
TGCGAATTGT CCGTTCTGGG CGCTTCCCTC TCTCCGCTAA GTTCCACCAG CTGTGCGGGA 
TTGGGTAACT TGCTAGGTCA ACTTGGAAGC CTGGGAGGCG GATTGGGAGG CACCCTGGGA 
GGAGTGGGCA GCACCTTGGG ATTGGGTGGC AATGGGGGAG GTGGTAAACG GGGTGGTCTC 
GGTCTTGGAA TTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608591.1
CDS	33..2030
Translation	MQSQLKRNTLLLLLLSVVILASCKSGGDAHVRVKRIVGGKQSKAPPVDDPVIFARLFDRDARVEGFRNPSTGIYSFLGMHYAEPPVGPLRYSRPVYKRLAGDFNATKHGPPCIQPHPQFPQRIIGDEDCLLLNVYTPQMPDETTGLPVFVWIHPGGYRYGSAAQYDATPMAQRGAIVVAPQYRLGSLGIMGDGTKQFDGNLAMFDLAAALRWVTDYISYFGGNPKQVQAIGHGSGAASAMYLSMSPTSRSAGDVHGVVAMSGTALSQYAMDKEPVQSVQEVAKINGCPTGNELEIVNCLRSKSAEDIIKNDDKVQTERLAGRALVKGLTGNVGFQPHIESEDDGRALPSLIVGEPEQQLKSSNFSGIPLLTGVTKHETANSVTVETIEKVFGSAEQFLGSLSDSLNKLTSFLKIDKLTGQIAKPELPGLTSVLTPTLQDVWKVPQALNVDQVLSKVVESTTDVLFNLPAVLTTQVWSRLAPAFMYSFEYNGTKSKGINFLKGLPIVSETAHDKPETVGHGDEIGYMFDANDIFGNPMEETRLTSAEDLKVRNNLIDLLVKFANKDKEEGGKSSIFQSVTGKATPFIKIDTKLQTSNDFRFCELSVLGASLSPLSSTSCAGLGNLLGQLGSLGGGLGGTLGGVGSTLGLGGNGGGGKRGGLGLGIL

Target ORF information:

RefSeq Version	NM_134747.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5397), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134747.3

ORF Insert Sequence:

ATGCAATCTC AGCTGAAGCG GAACACTTTG TTGCTACTGC TACTTTCGGT GGTGATCTTG 
GCCAGCTGCA AGTCTGGCGG GGATGCGCAC GTTCGCGTGA AGCGGATAGT GGGTGGCAAG 
CAGTCGAAGG CGCCGCCCGT CGATGATCCC GTGATCTTCG CGAGACTCTT TGATCGCGAT 
GCGCGAGTGG AGGGATTCCG GAATCCCAGC ACGGGCATCT ACTCCTTTCT GGGAATGCAC 
TACGCGGAGC CACCTGTTGG TCCACTGAGA TACTCCAGAC CGGTGTACAA AAGGCTGGCT 
GGTGACTTCA ATGCCACCAA ACATGGCCCA CCTTGTATAC AGCCGCATCC GCAGTTTCCT 
CAGAGGATTA TCGGCGATGA GGATTGCCTG CTGCTGAACG TCTACACCCC GCAAATGCCG 
GATGAGACCA CAGGGCTGCC AGTGTTCGTT TGGATTCACC CGGGTGGCTA TCGATATGGA 
TCGGCGGCCC AGTACGATGC CACGCCTATG GCCCAAAGGG GCGCAATCGT GGTGGCTCCT 
CAGTATCGCC TGGGATCACT GGGAATAATG GGGGATGGCA CCAAGCAGTT CGATGGAAAC 
CTGGCCATGT TCGACTTGGC CGCCGCTCTC CGTTGGGTCA CCGATTACAT CTCCTATTTC 
GGGGGCAATC CCAAGCAGGT CCAGGCCATA GGCCACGGAT CCGGAGCAGC CAGTGCCATG 
TATCTATCGA TGTCGCCGAC GTCGCGAAGC GCTGGCGATG TTCACGGAGT GGTGGCCATG 
TCGGGAACCG CTCTCTCTCA GTATGCCATG GACAAGGAAC CCGTTCAGAG CGTCCAGGAA 
GTGGCCAAAA TCAATGGCTG TCCCACAGGA AACGAGCTGG AAATCGTCAA TTGCTTAAGG 
AGTAAAAGTG CTGAGGACAT AATCAAGAAC GATGATAAAG TTCAAACGGA GCGTTTGGCA 
GGACGTGCCC TGGTCAAAGG ACTAACTGGA AACGTGGGCT TCCAGCCTCA CATCGAGAGC 
GAGGACGATG GTAGAGCATT GCCCAGTTTG ATAGTTGGAG AACCGGAGCA GCAGCTCAAG 
AGCAGTAACT TCTCGGGGAT TCCCCTGCTG ACTGGAGTCA CCAAGCACGA GACAGCAAAT 
TCCGTCACTG TGGAAACGAT AGAAAAGGTC TTTGGATCGG CGGAGCAGTT CCTGGGATCA 
CTAAGTGATT CTCTGAACAA GCTGACGAGT TTTCTGAAAA TCGACAAGCT AACCGGTCAG 
ATCGCCAAAC CGGAACTGCC GGGCCTCACG AGTGTCCTGA CTCCCACTCT CCAGGACGTT 
TGGAAGGTGC CGCAGGCGCT GAATGTGGAT CAAGTACTTT CCAAGGTGGT GGAATCAACG 
ACCGACGTGC TGTTCAACCT TCCGGCTGTT CTGACCACCC AGGTTTGGTC CAGGCTAGCT 
CCAGCCTTTA TGTACAGCTT CGAGTACAAT GGCACCAAGT CGAAGGGAAT AAACTTCCTG 
AAGGGTCTAC CAATTGTCTC GGAAACAGCC CATGATAAAC CGGAAACGGT GGGTCATGGT 
GATGAGATCG GCTACATGTT CGATGCAAAT GACATATTCG GGAATCCCAT GGAGGAGACT 
CGTTTGACCA GCGCCGAGGA TCTCAAAGTG CGCAACAACC TCATCGATCT GTTGGTCAAG 
TTTGCCAATA AGGACAAGGA AGAGGGCGGA AAGAGTTCCA TTTTCCAGAG TGTTACGGGA 
AAAGCCACGC CTTTTATTAA GATAGATACC AAACTGCAGA CCTCCAATGA TTTCCGCTTC 
TGCGAATTGT CCGTTCTGGG CGCTTCCCTC TCTCCGCTAA GTTCCACCAG CTGTGCGGGA 
TTGGGTAACT TGCTAGGTCA ACTTGGAAGC CTGGGAGGCG GATTGGGAGG CACCCTGGGA 
GGAGTGGGCA GCACCTTGGG ATTGGGTGGC AATGGGGGAG GTGGTAAACG GGGTGGTCTC 
GGTCTTGGAA TTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5397 ( NM_001272936.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259865.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG5397), mRNA.

Price & Availability↑

CloneID	OFa13447
Clone ID Related Accession (Same CDS sequence)	NM_134747.3 , NM_001272936.1
Accession Version	NM_001272936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCTC AGCTGAAGCG GAACACTTTG TTGCTACTGC TACTTTCGGT GGTGATCTTG 
GCCAGCTGCA AGTCTGGCGG GGATGCGCAC GTTCGCGTGA AGCGGATAGT GGGTGGCAAG 
CAGTCGAAGG CGCCGCCCGT CGATGATCCC GTGATCTTCG CGAGACTCTT TGATCGCGAT 
GCGCGAGTGG AGGGATTCCG GAATCCCAGC ACGGGCATCT ACTCCTTTCT GGGAATGCAC 
TACGCGGAGC CACCTGTTGG TCCACTGAGA TACTCCAGAC CGGTGTACAA AAGGCTGGCT 
GGTGACTTCA ATGCCACCAA ACATGGCCCA CCTTGTATAC AGCCGCATCC GCAGTTTCCT 
CAGAGGATTA TCGGCGATGA GGATTGCCTG CTGCTGAACG TCTACACCCC GCAAATGCCG 
GATGAGACCA CAGGGCTGCC AGTGTTCGTT TGGATTCACC CGGGTGGCTA TCGATATGGA 
TCGGCGGCCC AGTACGATGC CACGCCTATG GCCCAAAGGG GCGCAATCGT GGTGGCTCCT 
CAGTATCGCC TGGGATCACT GGGAATAATG GGGGATGGCA CCAAGCAGTT CGATGGAAAC 
CTGGCCATGT TCGACTTGGC CGCCGCTCTC CGTTGGGTCA CCGATTACAT CTCCTATTTC 
GGGGGCAATC CCAAGCAGGT CCAGGCCATA GGCCACGGAT CCGGAGCAGC CAGTGCCATG 
TATCTATCGA TGTCGCCGAC GTCGCGAAGC GCTGGCGATG TTCACGGAGT GGTGGCCATG 
TCGGGAACCG CTCTCTCTCA GTATGCCATG GACAAGGAAC CCGTTCAGAG CGTCCAGGAA 
GTGGCCAAAA TCAATGGCTG TCCCACAGGA AACGAGCTGG AAATCGTCAA TTGCTTAAGG 
AGTAAAAGTG CTGAGGACAT AATCAAGAAC GATGATAAAG TTCAAACGGA GCGTTTGGCA 
GGACGTGCCC TGGTCAAAGG ACTAACTGGA AACGTGGGCT TCCAGCCTCA CATCGAGAGC 
GAGGACGATG GTAGAGCATT GCCCAGTTTG ATAGTTGGAG AACCGGAGCA GCAGCTCAAG 
AGCAGTAACT TCTCGGGGAT TCCCCTGCTG ACTGGAGTCA CCAAGCACGA GACAGCAAAT 
TCCGTCACTG TGGAAACGAT AGAAAAGGTC TTTGGATCGG CGGAGCAGTT CCTGGGATCA 
CTAAGTGATT CTCTGAACAA GCTGACGAGT TTTCTGAAAA TCGACAAGCT AACCGGTCAG 
ATCGCCAAAC CGGAACTGCC GGGCCTCACG AGTGTCCTGA CTCCCACTCT CCAGGACGTT 
TGGAAGGTGC CGCAGGCGCT GAATGTGGAT CAAGTACTTT CCAAGGTGGT GGAATCAACG 
ACCGACGTGC TGTTCAACCT TCCGGCTGTT CTGACCACCC AGGTTTGGTC CAGGCTAGCT 
CCAGCCTTTA TGTACAGCTT CGAGTACAAT GGCACCAAGT CGAAGGGAAT AAACTTCCTG 
AAGGGTCTAC CAATTGTCTC GGAAACAGCC CATGATAAAC CGGAAACGGT GGGTCATGGT 
GATGAGATCG GCTACATGTT CGATGCAAAT GACATATTCG GGAATCCCAT GGAGGAGACT 
CGTTTGACCA GCGCCGAGGA TCTCAAAGTG CGCAACAACC TCATCGATCT GTTGGTCAAG 
TTTGCCAATA AGGACAAGGA AGAGGGCGGA AAGAGTTCCA TTTTCCAGAG TGTTACGGGA 
AAAGCCACGC CTTTTATTAA GATAGATACC AAACTGCAGA CCTCCAATGA TTTCCGCTTC 
TGCGAATTGT CCGTTCTGGG CGCTTCCCTC TCTCCGCTAA GTTCCACCAG CTGTGCGGGA 
TTGGGTAACT TGCTAGGTCA ACTTGGAAGC CTGGGAGGCG GATTGGGAGG CACCCTGGGA 
GGAGTGGGCA GCACCTTGGG ATTGGGTGGC AATGGGGGAG GTGGTAAACG GGGTGGTCTC 
GGTCTTGGAA TTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259865.1
CDS	33..2030
Translation	MQSQLKRNTLLLLLLSVVILASCKSGGDAHVRVKRIVGGKQSKAPPVDDPVIFARLFDRDARVEGFRNPSTGIYSFLGMHYAEPPVGPLRYSRPVYKRLAGDFNATKHGPPCIQPHPQFPQRIIGDEDCLLLNVYTPQMPDETTGLPVFVWIHPGGYRYGSAAQYDATPMAQRGAIVVAPQYRLGSLGIMGDGTKQFDGNLAMFDLAAALRWVTDYISYFGGNPKQVQAIGHGSGAASAMYLSMSPTSRSAGDVHGVVAMSGTALSQYAMDKEPVQSVQEVAKINGCPTGNELEIVNCLRSKSAEDIIKNDDKVQTERLAGRALVKGLTGNVGFQPHIESEDDGRALPSLIVGEPEQQLKSSNFSGIPLLTGVTKHETANSVTVETIEKVFGSAEQFLGSLSDSLNKLTSFLKIDKLTGQIAKPELPGLTSVLTPTLQDVWKVPQALNVDQVLSKVVESTTDVLFNLPAVLTTQVWSRLAPAFMYSFEYNGTKSKGINFLKGLPIVSETAHDKPETVGHGDEIGYMFDANDIFGNPMEETRLTSAEDLKVRNNLIDLLVKFANKDKEEGGKSSIFQSVTGKATPFIKIDTKLQTSNDFRFCELSVLGASLSPLSSTSCAGLGNLLGQLGSLGGGLGGTLGGVGSTLGLGGNGGGGKRGGLGLGIL

Target ORF information:

RefSeq Version	NM_001272936.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5397), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272936.1

ORF Insert Sequence:

ATGCAATCTC AGCTGAAGCG GAACACTTTG TTGCTACTGC TACTTTCGGT GGTGATCTTG 
GCCAGCTGCA AGTCTGGCGG GGATGCGCAC GTTCGCGTGA AGCGGATAGT GGGTGGCAAG 
CAGTCGAAGG CGCCGCCCGT CGATGATCCC GTGATCTTCG CGAGACTCTT TGATCGCGAT 
GCGCGAGTGG AGGGATTCCG GAATCCCAGC ACGGGCATCT ACTCCTTTCT GGGAATGCAC 
TACGCGGAGC CACCTGTTGG TCCACTGAGA TACTCCAGAC CGGTGTACAA AAGGCTGGCT 
GGTGACTTCA ATGCCACCAA ACATGGCCCA CCTTGTATAC AGCCGCATCC GCAGTTTCCT 
CAGAGGATTA TCGGCGATGA GGATTGCCTG CTGCTGAACG TCTACACCCC GCAAATGCCG 
GATGAGACCA CAGGGCTGCC AGTGTTCGTT TGGATTCACC CGGGTGGCTA TCGATATGGA 
TCGGCGGCCC AGTACGATGC CACGCCTATG GCCCAAAGGG GCGCAATCGT GGTGGCTCCT 
CAGTATCGCC TGGGATCACT GGGAATAATG GGGGATGGCA CCAAGCAGTT CGATGGAAAC 
CTGGCCATGT TCGACTTGGC CGCCGCTCTC CGTTGGGTCA CCGATTACAT CTCCTATTTC 
GGGGGCAATC CCAAGCAGGT CCAGGCCATA GGCCACGGAT CCGGAGCAGC CAGTGCCATG 
TATCTATCGA TGTCGCCGAC GTCGCGAAGC GCTGGCGATG TTCACGGAGT GGTGGCCATG 
TCGGGAACCG CTCTCTCTCA GTATGCCATG GACAAGGAAC CCGTTCAGAG CGTCCAGGAA 
GTGGCCAAAA TCAATGGCTG TCCCACAGGA AACGAGCTGG AAATCGTCAA TTGCTTAAGG 
AGTAAAAGTG CTGAGGACAT AATCAAGAAC GATGATAAAG TTCAAACGGA GCGTTTGGCA 
GGACGTGCCC TGGTCAAAGG ACTAACTGGA AACGTGGGCT TCCAGCCTCA CATCGAGAGC 
GAGGACGATG GTAGAGCATT GCCCAGTTTG ATAGTTGGAG AACCGGAGCA GCAGCTCAAG 
AGCAGTAACT TCTCGGGGAT TCCCCTGCTG ACTGGAGTCA CCAAGCACGA GACAGCAAAT 
TCCGTCACTG TGGAAACGAT AGAAAAGGTC TTTGGATCGG CGGAGCAGTT CCTGGGATCA 
CTAAGTGATT CTCTGAACAA GCTGACGAGT TTTCTGAAAA TCGACAAGCT AACCGGTCAG 
ATCGCCAAAC CGGAACTGCC GGGCCTCACG AGTGTCCTGA CTCCCACTCT CCAGGACGTT 
TGGAAGGTGC CGCAGGCGCT GAATGTGGAT CAAGTACTTT CCAAGGTGGT GGAATCAACG 
ACCGACGTGC TGTTCAACCT TCCGGCTGTT CTGACCACCC AGGTTTGGTC CAGGCTAGCT 
CCAGCCTTTA TGTACAGCTT CGAGTACAAT GGCACCAAGT CGAAGGGAAT AAACTTCCTG 
AAGGGTCTAC CAATTGTCTC GGAAACAGCC CATGATAAAC CGGAAACGGT GGGTCATGGT 
GATGAGATCG GCTACATGTT CGATGCAAAT GACATATTCG GGAATCCCAT GGAGGAGACT 
CGTTTGACCA GCGCCGAGGA TCTCAAAGTG CGCAACAACC TCATCGATCT GTTGGTCAAG 
TTTGCCAATA AGGACAAGGA AGAGGGCGGA AAGAGTTCCA TTTTCCAGAG TGTTACGGGA 
AAAGCCACGC CTTTTATTAA GATAGATACC AAACTGCAGA CCTCCAATGA TTTCCGCTTC 
TGCGAATTGT CCGTTCTGGG CGCTTCCCTC TCTCCGCTAA GTTCCACCAG CTGTGCGGGA 
TTGGGTAACT TGCTAGGTCA ACTTGGAAGC CTGGGAGGCG GATTGGGAGG CACCCTGGGA 
GGAGTGGGCA GCACCTTGGG ATTGGGTGGC AATGGGGGAG GTGGTAAACG GGGTGGTCTC 
GGTCTTGGAA TTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5397 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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