PNUTS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNUTS
Entrez Gene ID	33270
Full Name	CG33526 gene product from transcript CG33526-RD
Synonyms	CG31657,CG33526,CG4124,Dmel\CG33526,PNUTS-RB,PNUTSDm,Pnuts
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21E2-21E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014455.2	NP_001014455.1	PNUTS, isoform B
NM_001014454.2	NP_001014454.1	PNUTS, isoform A
NM_001272921.1	NP_001259850.1	PNUTS, isoform E
NM_001014453.2	NP_001014453.1	PNUTS, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PNUTS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNUTS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13409	NM_001272921.1 Latest version!	Drosophila melanogaster PNUTS, transcript variant E (PNUTS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa13409	NM_001014453.2 Latest version!	Drosophila melanogaster PNUTS, transcript variant D (PNUTS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa13410	NM_001014455.2 Latest version!	Drosophila melanogaster PNUTS, transcript variant B (PNUTS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa13410	NM_001014454.2 Latest version!	Drosophila melanogaster PNUTS, transcript variant A (PNUTS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNUTS ( NM_001272921.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259850.1 Drosophila melanogaster PNUTS, transcript variant E (PNUTS), mRNA.

Price & Availability↑

CloneID	OFa13409
Clone ID Related Accession (Same CDS sequence)	NM_001272921.1 , NM_001014453.2
Accession Version	NM_001272921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PNUTS, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTCGTA TAGTTCCACT GCAATTGCTG AGGTGCCTGC GGGTGCTTTT GGACAATAAT 
GGTGGAATTT TAAGTGCAGT GGAGGTGAAA CGCATTTCGG GTTTGATGGC CAAGTATTCA 
AAAAAACTCG TCTCGAAATG TGTGTATGTA CAAATTCTGA AATCCACAAA GACGGAACTA 
TTGGGCGACT TTATGGCGGT GGGCGGTTGG TCGCTGGTCT ACACCTGGTT AAATGATGCC 
ATCAGGGCCA TGAACTGGCC GCTGGTACAG GAGATCCTGG AACTGCTGCT CCTTTCTCCC 
GTGGATGTCA ATCGCCTCAA GATAAACTCA GCACCTATTC TGGTCAAGGG GCTGTGCAAG 
GATGGCGGCA ACGAAGGTGT ACGCATTCTA GCCAAGCGTT TGGTAGAGCA GTGGCTGAAG 
ATTGTGACGG AAAACACAAG CATGACGATC CAAGGTGCTG CGCCACATAT TGCAGCCACT 
ACTGCCTCTC AGGTTCCTGC ATCCGTTTCT GGAGCTGGCT CCGCTTCAGT TCCGGACTCT 
GCCTCTTCTT CAGACAGCAC TGATAGCGCC GGAGCACCTG TGACGTATAC CATCACCTCG 
GCGGCCAACA GTAGCAATTC CAACAGCATT ACCATCAAGT GGAAGCCTTT GTTGGACAAC 
CCAGATGGTG GCGACGAAAC GGATTCTGGC ACACAATCGA AAGACAACGA GGTGGAACAT 
CTGGAAAAGG GCTCCTCCGC AACCCACTTA TTAGCAGATG CTAGTCTGGG GAAAAAGTCC 
GGGTTAGATG ATAGTAGCTC CGCGGCGGAG GTTAAGTCTG GGGAGGACGT GGATAATAAG 
AAGCATAAGA GCTCAAAGAG CGACAAAAGT AAACGGTCCG AGAAGGAGCG CGAGAAGGAT 
AGAAAGAAGG AAAGTTCAAG TCATAGGTCC TCCTCATCTA GCCATAAGTC GTCCTCTTCC 
TCGTCATCCT CCTCTTCGAA GAGCAGTTCT TCCAAGAGCT CCTCATCTTC TTCCTCTTCC 
CATCGCAGTT CTAGCGATAA ATACCGCGAT AAAGACAAGG CTCGCTCAAG TTCCGGCTCC 
TCCAGCTCGA GCAAGGACCG GGAGCGCAGC AGCGGTTCGT CTTCTTCTTC CTCGAATAAA 
CACAAGTCCT CAAAGTCCTC CTCGTCGTCG TCGTCGTCGT CGTCGAGCAG CTCTGGTTCT 
AGTTCGTCCA AAGATCGCAA GTCGTCGTCG TCAACTTCGT CATCATCCTC ATCCTCGAGG 
CAGGACAAGG ACAAGGACAA CAAGCTAATG CCTCCGCCAG CTGTGGCAGC ATCAAATGCT 
GCAGGTACAT CTCCTACAGC TTCGGCAAAG GAGAGCGACG CACAGAAGCC CAGATCGATT 
CCTATCATGT CCCGCAAGGC CTCCATTTCG ATAGAAATTC GCCGGGACAC CGAAAAGACG 
GCCACTGTAA AGACGTACCA GTCCAAATTC AGATCGCATG GCCTGACGGA AGAGGCTCCG 
CCTCCCCCAT CTCGCAAGGG CCTAAAGAAG CCCACATCGT CGGCGGTTGC CGCTTCACCC 
GCTTTAATGG CAGTCCCGTC TTCACTGAAG CGACCATCGC CGCCACCCAG GGATTTGTCC 
ACCGACAAGA AGGCCAAGAT CGACATCAAC AACGTCGCGG GACATGTGGA GAGGCCCGGA 
GCCGCCAAGC TGATAGCGCC CAAAAAGATT CAAACCCTGG TGGAGACCAA CCTGTTCTCG 
GACGCCCTGG CCGCCTCCAT CGAACCCAAA AAGGTGGTGA AGCGCAAGCG TACGTCTGCA 
ACATCGCCCA CGGACAAGGA CGCGCCACTG GCGCCGCTTA AGTTCTACCA GGATACTCTG 
GACGAGTCGA AGAGCGAGGA GAAGTCGGAC GACAGCAGCA AGGAAAACGA TGATGCGCGC 
TCCTCGTCAC CTGGCAAGGA CACGGATGCC GATGACGACG ACATTCCCCT TAAGAGGGTC 
AAGGAGGACA TCGAGCAAAA GGTGCAGAAG GCGAAGGCTG CGGCAGGCGG AGAAGGCGGA 
GAAGCGGGTA ACACCTCTGA CAACGCTGAA GATCTTCCCG AGGAGCCCAA GAAGCCTGGA 
CCTGGCTGTG GACCCGATGG ACCACCGGGA GTGCTCACGC TGCACCGCCG CAAGGGCCCG 
AAAAAGAAGC TAACCTGGCA GTCAGAGGAA AAGCTCACCC AGATACGATA CTTCGAGGTC 
GATCGCTCAG AGCGCGTAAA TGTGATGAAG CAGACCTTCC TGGAAATGAA GAACATGGAG 
CGATTCAGCG AGCGGGACGC GCTGACCATT GCTCGGCGTG GATTCGACGA TACCATGGAG 
CCGCAAATGG AGTGGCGGCC ACTACTCGAA GTCGATAATG TGCCCGACCA CCCGAACGGA 
AACCTCTCCA AGCAGCGCCA GGTGCAGATG GACAGGGAGG CCACCACTCT AAGAGCCCTG 
TACTTCTCGC CCGACATGAT TCCCGACAGT CCTGCGGAGG CGGAACTGGA ACCGCATTTT 
GCTCATGACA TTCCCGTGAT ACCCATGGAT GACCTGACGG GGAATCCGGA TGCTGTCAAC 
GACTACAGTG CCTTGGCGTG GCCGGAGCCC AAGAAATACG CGCCTAGCTC GGGAGTCGGA 
GCAAATATCA GCGGATCGGA CATGGGCTTC AATGACAATA TGTTGACCGG ACTGGGAGCT 
GGACCGAACC TAATGCAAAA TCCATTCGAT CCGTTCATGG GAGGCATGCC TGCTCCCACG 
GGAATGCCAC CCGGACCAAA CATGATGCCA AATCAAGTGC CCAACGGAAC GCCGCAAATC 
TGGCAGAACC AAATGGGTCC GGTAGGTGGG CCGCCCGGTG TACCGCCCAT GATGAACGGA 
CCCGGTGGCC CCGGAATGGA TATGAGCAAT ATGAACATGA ACAACATGCC ACCGCAAAAC 
TTTATGGGCA ATCAGTTTGG AAGTCCAGTG TCTCCATTCG GAAGCGGCCC ACCACCTGGA 
TTCAATCAGT ACCCGCCCAT GATGATGAAT GGACCAGGTC CGGGACCGGT AATGGGACCC 
AATGGTCCAG TGATGGGACC CAACGGCCCC ATGATGAACG GCCCACCCAA TGGACCCATG 
ATGAACGGGC CGCCCAATGG AATGGGGCCA GGACTGATGA TGGGCGGACC TAGGAATAAC 
AACAACAACA ACAACAGGAA CAAAAACAAC GGCGGCGGCA TCTGGCGCAG CGGGGGCAAC 
AACTTCCAGG AGAACTCCGG CGGCAACTGG CGCTCAGCAG GATCTGGCCC CAATCGAGGA 
GGTGGAGGAG GCAATGGCAA CACCGGCGTC TGCAAGCAGT TCATGCGAGG ACACTGCAAC 
ATGGGCAAGA ACTGCAAGTA TGTGCATCCG CAGAAGAAGC GCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259850.1
CDS	322..3729
Translation	MPRIVPLQLLRCLRVLLDNNGGILSAVEVKRISGLMAKYSKKLVSKCVYVQILKSTKTELLGDFMAVGGWSLVYTWLNDAIRAMNWPLVQEILELLLLSPVDVNRLKINSAPILVKGLCKDGGNEGVRILAKRLVEQWLKIVTENTSMTIQGAAPHIAATTASQVPASVSGAGSASVPDSASSSDSTDSAGAPVTYTITSAANSSNSNSITIKWKPLLDNPDGGDETDSGTQSKDNEVEHLEKGSSATHLLADASLGKKSGLDDSSSAAEVKSGEDVDNKKHKSSKSDKSKRSEKEREKDRKKESSSHRSSSSSHKSSSSSSSSSSKSSSSKSSSSSSSSHRSSSDKYRDKDKARSSSGSSSSSKDRERSSGSSSSSSNKHKSSKSSSSSSSSSSSSSGSSSSKDRKSSSSTSSSSSSSRQDKDKDNKLMPPPAVAASNAAGTSPTASAKESDAQKPRSIPIMSRKASISIEIRRDTEKTATVKTYQSKFRSHGLTEEAPPPPSRKGLKKPTSSAVAASPALMAVPSSLKRPSPPPRDLSTDKKAKIDINNVAGHVERPGAAKLIAPKKIQTLVETNLFSDALAASIEPKKVVKRKRTSATSPTDKDAPLAPLKFYQDTLDESKSEEKSDDSSKENDDARSSSPGKDTDADDDDIPLKRVKEDIEQKVQKAKAAAGGEGGEAGNTSDNAEDLPEEPKKPGPGCGPDGPPGVLTLHRRKGPKKKLTWQSEEKLTQIRYFEVDRSERVNVMKQTFLEMKNMERFSERDALTIARRGFDDTMEPQMEWRPLLEVDNVPDHPNGNLSKQRQVQMDREATTLRALYFSPDMIPDSPAEAELEPHFAHDIPVIPMDDLTGNPDAVNDYSALAWPEPKKYAPSSGVGANISGSDMGFNDNMLTGLGAGPNLMQNPFDPFMGGMPAPTGMPPGPNMMPNQVPNGTPQIWQNQMGPVGGPPGVPPMMNGPGGPGMDMSNMNMNNMPPQNFMGNQFGSPVSPFGSGPPPGFNQYPPMMMNGPGPGPVMGPNGPVMGPNGPMMNGPPNGPMMNGPPNGMGPGLMMGGPRNNNNNNNRNKNNGGGIWRSGGNNFQENSGGNWRSAGSGPNRGGGGGNGNTGVCKQFMRGHCNMGKNCKYVHPQKKRL

Target ORF information:

RefSeq Version	NM_001272921.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PNUTS, transcript variant E (PNUTS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272921.1

ORF Insert Sequence:

ATGCCTCGTA TAGTTCCACT GCAATTGCTG AGGTGCCTGC GGGTGCTTTT GGACAATAAT 
GGTGGAATTT TAAGTGCAGT GGAGGTGAAA CGCATTTCGG GTTTGATGGC CAAGTATTCA 
AAAAAACTCG TCTCGAAATG TGTGTATGTA CAAATTCTGA AATCCACAAA GACGGAACTA 
TTGGGCGACT TTATGGCGGT GGGCGGTTGG TCGCTGGTCT ACACCTGGTT AAATGATGCC 
ATCAGGGCCA TGAACTGGCC GCTGGTACAG GAGATCCTGG AACTGCTGCT CCTTTCTCCC 
GTGGATGTCA ATCGCCTCAA GATAAACTCA GCACCTATTC TGGTCAAGGG GCTGTGCAAG 
GATGGCGGCA ACGAAGGTGT ACGCATTCTA GCCAAGCGTT TGGTAGAGCA GTGGCTGAAG 
ATTGTGACGG AAAACACAAG CATGACGATC CAAGGTGCTG CGCCACATAT TGCAGCCACT 
ACTGCCTCTC AGGTTCCTGC ATCCGTTTCT GGAGCTGGCT CCGCTTCAGT TCCGGACTCT 
GCCTCTTCTT CAGACAGCAC TGATAGCGCC GGAGCACCTG TGACGTATAC CATCACCTCG 
GCGGCCAACA GTAGCAATTC CAACAGCATT ACCATCAAGT GGAAGCCTTT GTTGGACAAC 
CCAGATGGTG GCGACGAAAC GGATTCTGGC ACACAATCGA AAGACAACGA GGTGGAACAT 
CTGGAAAAGG GCTCCTCCGC AACCCACTTA TTAGCAGATG CTAGTCTGGG GAAAAAGTCC 
GGGTTAGATG ATAGTAGCTC CGCGGCGGAG GTTAAGTCTG GGGAGGACGT GGATAATAAG 
AAGCATAAGA GCTCAAAGAG CGACAAAAGT AAACGGTCCG AGAAGGAGCG CGAGAAGGAT 
AGAAAGAAGG AAAGTTCAAG TCATAGGTCC TCCTCATCTA GCCATAAGTC GTCCTCTTCC 
TCGTCATCCT CCTCTTCGAA GAGCAGTTCT TCCAAGAGCT CCTCATCTTC TTCCTCTTCC 
CATCGCAGTT CTAGCGATAA ATACCGCGAT AAAGACAAGG CTCGCTCAAG TTCCGGCTCC 
TCCAGCTCGA GCAAGGACCG GGAGCGCAGC AGCGGTTCGT CTTCTTCTTC CTCGAATAAA 
CACAAGTCCT CAAAGTCCTC CTCGTCGTCG TCGTCGTCGT CGTCGAGCAG CTCTGGTTCT 
AGTTCGTCCA AAGATCGCAA GTCGTCGTCG TCAACTTCGT CATCATCCTC ATCCTCGAGG 
CAGGACAAGG ACAAGGACAA CAAGCTAATG CCTCCGCCAG CTGTGGCAGC ATCAAATGCT 
GCAGGTACAT CTCCTACAGC TTCGGCAAAG GAGAGCGACG CACAGAAGCC CAGATCGATT 
CCTATCATGT CCCGCAAGGC CTCCATTTCG ATAGAAATTC GCCGGGACAC CGAAAAGACG 
GCCACTGTAA AGACGTACCA GTCCAAATTC AGATCGCATG GCCTGACGGA AGAGGCTCCG 
CCTCCCCCAT CTCGCAAGGG CCTAAAGAAG CCCACATCGT CGGCGGTTGC CGCTTCACCC 
GCTTTAATGG CAGTCCCGTC TTCACTGAAG CGACCATCGC CGCCACCCAG GGATTTGTCC 
ACCGACAAGA AGGCCAAGAT CGACATCAAC AACGTCGCGG GACATGTGGA GAGGCCCGGA 
GCCGCCAAGC TGATAGCGCC CAAAAAGATT CAAACCCTGG TGGAGACCAA CCTGTTCTCG 
GACGCCCTGG CCGCCTCCAT CGAACCCAAA AAGGTGGTGA AGCGCAAGCG TACGTCTGCA 
ACATCGCCCA CGGACAAGGA CGCGCCACTG GCGCCGCTTA AGTTCTACCA GGATACTCTG 
GACGAGTCGA AGAGCGAGGA GAAGTCGGAC GACAGCAGCA AGGAAAACGA TGATGCGCGC 
TCCTCGTCAC CTGGCAAGGA CACGGATGCC GATGACGACG ACATTCCCCT TAAGAGGGTC 
AAGGAGGACA TCGAGCAAAA GGTGCAGAAG GCGAAGGCTG CGGCAGGCGG AGAAGGCGGA 
GAAGCGGGTA ACACCTCTGA CAACGCTGAA GATCTTCCCG AGGAGCCCAA GAAGCCTGGA 
CCTGGCTGTG GACCCGATGG ACCACCGGGA GTGCTCACGC TGCACCGCCG CAAGGGCCCG 
AAAAAGAAGC TAACCTGGCA GTCAGAGGAA AAGCTCACCC AGATACGATA CTTCGAGGTC 
GATCGCTCAG AGCGCGTAAA TGTGATGAAG CAGACCTTCC TGGAAATGAA GAACATGGAG 
CGATTCAGCG AGCGGGACGC GCTGACCATT GCTCGGCGTG GATTCGACGA TACCATGGAG 
CCGCAAATGG AGTGGCGGCC ACTACTCGAA GTCGATAATG TGCCCGACCA CCCGAACGGA 
AACCTCTCCA AGCAGCGCCA GGTGCAGATG GACAGGGAGG CCACCACTCT AAGAGCCCTG 
TACTTCTCGC CCGACATGAT TCCCGACAGT CCTGCGGAGG CGGAACTGGA ACCGCATTTT 
GCTCATGACA TTCCCGTGAT ACCCATGGAT GACCTGACGG GGAATCCGGA TGCTGTCAAC 
GACTACAGTG CCTTGGCGTG GCCGGAGCCC AAGAAATACG CGCCTAGCTC GGGAGTCGGA 
GCAAATATCA GCGGATCGGA CATGGGCTTC AATGACAATA TGTTGACCGG ACTGGGAGCT 
GGACCGAACC TAATGCAAAA TCCATTCGAT CCGTTCATGG GAGGCATGCC TGCTCCCACG 
GGAATGCCAC CCGGACCAAA CATGATGCCA AATCAAGTGC CCAACGGAAC GCCGCAAATC 
TGGCAGAACC AAATGGGTCC GGTAGGTGGG CCGCCCGGTG TACCGCCCAT GATGAACGGA 
CCCGGTGGCC CCGGAATGGA TATGAGCAAT ATGAACATGA ACAACATGCC ACCGCAAAAC 
TTTATGGGCA ATCAGTTTGG AAGTCCAGTG TCTCCATTCG GAAGCGGCCC ACCACCTGGA 
TTCAATCAGT ACCCGCCCAT GATGATGAAT GGACCAGGTC CGGGACCGGT AATGGGACCC 
AATGGTCCAG TGATGGGACC CAACGGCCCC ATGATGAACG GCCCACCCAA TGGACCCATG 
ATGAACGGGC CGCCCAATGG AATGGGGCCA GGACTGATGA TGGGCGGACC TAGGAATAAC 
AACAACAACA ACAACAGGAA CAAAAACAAC GGCGGCGGCA TCTGGCGCAG CGGGGGCAAC 
AACTTCCAGG AGAACTCCGG CGGCAACTGG CGCTCAGCAG GATCTGGCCC CAATCGAGGA 
GGTGGAGGAG GCAATGGCAA CACCGGCGTC TGCAAGCAGT TCATGCGAGG ACACTGCAAC 
ATGGGCAAGA ACTGCAAGTA TGTGCATCCG CAGAAGAAGC GCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNUTS ( NM_001014453.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014453.1 Drosophila melanogaster PNUTS, transcript variant D (PNUTS), mRNA.

Price & Availability↑

CloneID	OFa13409
Clone ID Related Accession (Same CDS sequence)	NM_001272921.1 , NM_001014453.2
Accession Version	NM_001014453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PNUTS, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001014453.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTCGTA TAGTTCCACT GCAATTGCTG AGGTGCCTGC GGGTGCTTTT GGACAATAAT 
GGTGGAATTT TAAGTGCAGT GGAGGTGAAA CGCATTTCGG GTTTGATGGC CAAGTATTCA 
AAAAAACTCG TCTCGAAATG TGTGTATGTA CAAATTCTGA AATCCACAAA GACGGAACTA 
TTGGGCGACT TTATGGCGGT GGGCGGTTGG TCGCTGGTCT ACACCTGGTT AAATGATGCC 
ATCAGGGCCA TGAACTGGCC GCTGGTACAG GAGATCCTGG AACTGCTGCT CCTTTCTCCC 
GTGGATGTCA ATCGCCTCAA GATAAACTCA GCACCTATTC TGGTCAAGGG GCTGTGCAAG 
GATGGCGGCA ACGAAGGTGT ACGCATTCTA GCCAAGCGTT TGGTAGAGCA GTGGCTGAAG 
ATTGTGACGG AAAACACAAG CATGACGATC CAAGGTGCTG CGCCACATAT TGCAGCCACT 
ACTGCCTCTC AGGTTCCTGC ATCCGTTTCT GGAGCTGGCT CCGCTTCAGT TCCGGACTCT 
GCCTCTTCTT CAGACAGCAC TGATAGCGCC GGAGCACCTG TGACGTATAC CATCACCTCG 
GCGGCCAACA GTAGCAATTC CAACAGCATT ACCATCAAGT GGAAGCCTTT GTTGGACAAC 
CCAGATGGTG GCGACGAAAC GGATTCTGGC ACACAATCGA AAGACAACGA GGTGGAACAT 
CTGGAAAAGG GCTCCTCCGC AACCCACTTA TTAGCAGATG CTAGTCTGGG GAAAAAGTCC 
GGGTTAGATG ATAGTAGCTC CGCGGCGGAG GTTAAGTCTG GGGAGGACGT GGATAATAAG 
AAGCATAAGA GCTCAAAGAG CGACAAAAGT AAACGGTCCG AGAAGGAGCG CGAGAAGGAT 
AGAAAGAAGG AAAGTTCAAG TCATAGGTCC TCCTCATCTA GCCATAAGTC GTCCTCTTCC 
TCGTCATCCT CCTCTTCGAA GAGCAGTTCT TCCAAGAGCT CCTCATCTTC TTCCTCTTCC 
CATCGCAGTT CTAGCGATAA ATACCGCGAT AAAGACAAGG CTCGCTCAAG TTCCGGCTCC 
TCCAGCTCGA GCAAGGACCG GGAGCGCAGC AGCGGTTCGT CTTCTTCTTC CTCGAATAAA 
CACAAGTCCT CAAAGTCCTC CTCGTCGTCG TCGTCGTCGT CGTCGAGCAG CTCTGGTTCT 
AGTTCGTCCA AAGATCGCAA GTCGTCGTCG TCAACTTCGT CATCATCCTC ATCCTCGAGG 
CAGGACAAGG ACAAGGACAA CAAGCTAATG CCTCCGCCAG CTGTGGCAGC ATCAAATGCT 
GCAGGTACAT CTCCTACAGC TTCGGCAAAG GAGAGCGACG CACAGAAGCC CAGATCGATT 
CCTATCATGT CCCGCAAGGC CTCCATTTCG ATAGAAATTC GCCGGGACAC CGAAAAGACG 
GCCACTGTAA AGACGTACCA GTCCAAATTC AGATCGCATG GCCTGACGGA AGAGGCTCCG 
CCTCCCCCAT CTCGCAAGGG CCTAAAGAAG CCCACATCGT CGGCGGTTGC CGCTTCACCC 
GCTTTAATGG CAGTCCCGTC TTCACTGAAG CGACCATCGC CGCCACCCAG GGATTTGTCC 
ACCGACAAGA AGGCCAAGAT CGACATCAAC AACGTCGCGG GACATGTGGA GAGGCCCGGA 
GCCGCCAAGC TGATAGCGCC CAAAAAGATT CAAACCCTGG TGGAGACCAA CCTGTTCTCG 
GACGCCCTGG CCGCCTCCAT CGAACCCAAA AAGGTGGTGA AGCGCAAGCG TACGTCTGCA 
ACATCGCCCA CGGACAAGGA CGCGCCACTG GCGCCGCTTA AGTTCTACCA GGATACTCTG 
GACGAGTCGA AGAGCGAGGA GAAGTCGGAC GACAGCAGCA AGGAAAACGA TGATGCGCGC 
TCCTCGTCAC CTGGCAAGGA CACGGATGCC GATGACGACG ACATTCCCCT TAAGAGGGTC 
AAGGAGGACA TCGAGCAAAA GGTGCAGAAG GCGAAGGCTG CGGCAGGCGG AGAAGGCGGA 
GAAGCGGGTA ACACCTCTGA CAACGCTGAA GATCTTCCCG AGGAGCCCAA GAAGCCTGGA 
CCTGGCTGTG GACCCGATGG ACCACCGGGA GTGCTCACGC TGCACCGCCG CAAGGGCCCG 
AAAAAGAAGC TAACCTGGCA GTCAGAGGAA AAGCTCACCC AGATACGATA CTTCGAGGTC 
GATCGCTCAG AGCGCGTAAA TGTGATGAAG CAGACCTTCC TGGAAATGAA GAACATGGAG 
CGATTCAGCG AGCGGGACGC GCTGACCATT GCTCGGCGTG GATTCGACGA TACCATGGAG 
CCGCAAATGG AGTGGCGGCC ACTACTCGAA GTCGATAATG TGCCCGACCA CCCGAACGGA 
AACCTCTCCA AGCAGCGCCA GGTGCAGATG GACAGGGAGG CCACCACTCT AAGAGCCCTG 
TACTTCTCGC CCGACATGAT TCCCGACAGT CCTGCGGAGG CGGAACTGGA ACCGCATTTT 
GCTCATGACA TTCCCGTGAT ACCCATGGAT GACCTGACGG GGAATCCGGA TGCTGTCAAC 
GACTACAGTG CCTTGGCGTG GCCGGAGCCC AAGAAATACG CGCCTAGCTC GGGAGTCGGA 
GCAAATATCA GCGGATCGGA CATGGGCTTC AATGACAATA TGTTGACCGG ACTGGGAGCT 
GGACCGAACC TAATGCAAAA TCCATTCGAT CCGTTCATGG GAGGCATGCC TGCTCCCACG 
GGAATGCCAC CCGGACCAAA CATGATGCCA AATCAAGTGC CCAACGGAAC GCCGCAAATC 
TGGCAGAACC AAATGGGTCC GGTAGGTGGG CCGCCCGGTG TACCGCCCAT GATGAACGGA 
CCCGGTGGCC CCGGAATGGA TATGAGCAAT ATGAACATGA ACAACATGCC ACCGCAAAAC 
TTTATGGGCA ATCAGTTTGG AAGTCCAGTG TCTCCATTCG GAAGCGGCCC ACCACCTGGA 
TTCAATCAGT ACCCGCCCAT GATGATGAAT GGACCAGGTC CGGGACCGGT AATGGGACCC 
AATGGTCCAG TGATGGGACC CAACGGCCCC ATGATGAACG GCCCACCCAA TGGACCCATG 
ATGAACGGGC CGCCCAATGG AATGGGGCCA GGACTGATGA TGGGCGGACC TAGGAATAAC 
AACAACAACA ACAACAGGAA CAAAAACAAC GGCGGCGGCA TCTGGCGCAG CGGGGGCAAC 
AACTTCCAGG AGAACTCCGG CGGCAACTGG CGCTCAGCAG GATCTGGCCC CAATCGAGGA 
GGTGGAGGAG GCAATGGCAA CACCGGCGTC TGCAAGCAGT TCATGCGAGG ACACTGCAAC 
ATGGGCAAGA ACTGCAAGTA TGTGCATCCG CAGAAGAAGC GCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014453.1
CDS	1110..4517
Translation	MPRIVPLQLLRCLRVLLDNNGGILSAVEVKRISGLMAKYSKKLVSKCVYVQILKSTKTELLGDFMAVGGWSLVYTWLNDAIRAMNWPLVQEILELLLLSPVDVNRLKINSAPILVKGLCKDGGNEGVRILAKRLVEQWLKIVTENTSMTIQGAAPHIAATTASQVPASVSGAGSASVPDSASSSDSTDSAGAPVTYTITSAANSSNSNSITIKWKPLLDNPDGGDETDSGTQSKDNEVEHLEKGSSATHLLADASLGKKSGLDDSSSAAEVKSGEDVDNKKHKSSKSDKSKRSEKEREKDRKKESSSHRSSSSSHKSSSSSSSSSSKSSSSKSSSSSSSSHRSSSDKYRDKDKARSSSGSSSSSKDRERSSGSSSSSSNKHKSSKSSSSSSSSSSSSSGSSSSKDRKSSSSTSSSSSSSRQDKDKDNKLMPPPAVAASNAAGTSPTASAKESDAQKPRSIPIMSRKASISIEIRRDTEKTATVKTYQSKFRSHGLTEEAPPPPSRKGLKKPTSSAVAASPALMAVPSSLKRPSPPPRDLSTDKKAKIDINNVAGHVERPGAAKLIAPKKIQTLVETNLFSDALAASIEPKKVVKRKRTSATSPTDKDAPLAPLKFYQDTLDESKSEEKSDDSSKENDDARSSSPGKDTDADDDDIPLKRVKEDIEQKVQKAKAAAGGEGGEAGNTSDNAEDLPEEPKKPGPGCGPDGPPGVLTLHRRKGPKKKLTWQSEEKLTQIRYFEVDRSERVNVMKQTFLEMKNMERFSERDALTIARRGFDDTMEPQMEWRPLLEVDNVPDHPNGNLSKQRQVQMDREATTLRALYFSPDMIPDSPAEAELEPHFAHDIPVIPMDDLTGNPDAVNDYSALAWPEPKKYAPSSGVGANISGSDMGFNDNMLTGLGAGPNLMQNPFDPFMGGMPAPTGMPPGPNMMPNQVPNGTPQIWQNQMGPVGGPPGVPPMMNGPGGPGMDMSNMNMNNMPPQNFMGNQFGSPVSPFGSGPPPGFNQYPPMMMNGPGPGPVMGPNGPVMGPNGPMMNGPPNGPMMNGPPNGMGPGLMMGGPRNNNNNNNRNKNNGGGIWRSGGNNFQENSGGNWRSAGSGPNRGGGGGNGNTGVCKQFMRGHCNMGKNCKYVHPQKKRL

Target ORF information:

RefSeq Version	NM_001014453.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PNUTS, transcript variant D (PNUTS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014453.2

ORF Insert Sequence:

ATGCCTCGTA TAGTTCCACT GCAATTGCTG AGGTGCCTGC GGGTGCTTTT GGACAATAAT 
GGTGGAATTT TAAGTGCAGT GGAGGTGAAA CGCATTTCGG GTTTGATGGC CAAGTATTCA 
AAAAAACTCG TCTCGAAATG TGTGTATGTA CAAATTCTGA AATCCACAAA GACGGAACTA 
TTGGGCGACT TTATGGCGGT GGGCGGTTGG TCGCTGGTCT ACACCTGGTT AAATGATGCC 
ATCAGGGCCA TGAACTGGCC GCTGGTACAG GAGATCCTGG AACTGCTGCT CCTTTCTCCC 
GTGGATGTCA ATCGCCTCAA GATAAACTCA GCACCTATTC TGGTCAAGGG GCTGTGCAAG 
GATGGCGGCA ACGAAGGTGT ACGCATTCTA GCCAAGCGTT TGGTAGAGCA GTGGCTGAAG 
ATTGTGACGG AAAACACAAG CATGACGATC CAAGGTGCTG CGCCACATAT TGCAGCCACT 
ACTGCCTCTC AGGTTCCTGC ATCCGTTTCT GGAGCTGGCT CCGCTTCAGT TCCGGACTCT 
GCCTCTTCTT CAGACAGCAC TGATAGCGCC GGAGCACCTG TGACGTATAC CATCACCTCG 
GCGGCCAACA GTAGCAATTC CAACAGCATT ACCATCAAGT GGAAGCCTTT GTTGGACAAC 
CCAGATGGTG GCGACGAAAC GGATTCTGGC ACACAATCGA AAGACAACGA GGTGGAACAT 
CTGGAAAAGG GCTCCTCCGC AACCCACTTA TTAGCAGATG CTAGTCTGGG GAAAAAGTCC 
GGGTTAGATG ATAGTAGCTC CGCGGCGGAG GTTAAGTCTG GGGAGGACGT GGATAATAAG 
AAGCATAAGA GCTCAAAGAG CGACAAAAGT AAACGGTCCG AGAAGGAGCG CGAGAAGGAT 
AGAAAGAAGG AAAGTTCAAG TCATAGGTCC TCCTCATCTA GCCATAAGTC GTCCTCTTCC 
TCGTCATCCT CCTCTTCGAA GAGCAGTTCT TCCAAGAGCT CCTCATCTTC TTCCTCTTCC 
CATCGCAGTT CTAGCGATAA ATACCGCGAT AAAGACAAGG CTCGCTCAAG TTCCGGCTCC 
TCCAGCTCGA GCAAGGACCG GGAGCGCAGC AGCGGTTCGT CTTCTTCTTC CTCGAATAAA 
CACAAGTCCT CAAAGTCCTC CTCGTCGTCG TCGTCGTCGT CGTCGAGCAG CTCTGGTTCT 
AGTTCGTCCA AAGATCGCAA GTCGTCGTCG TCAACTTCGT CATCATCCTC ATCCTCGAGG 
CAGGACAAGG ACAAGGACAA CAAGCTAATG CCTCCGCCAG CTGTGGCAGC ATCAAATGCT 
GCAGGTACAT CTCCTACAGC TTCGGCAAAG GAGAGCGACG CACAGAAGCC CAGATCGATT 
CCTATCATGT CCCGCAAGGC CTCCATTTCG ATAGAAATTC GCCGGGACAC CGAAAAGACG 
GCCACTGTAA AGACGTACCA GTCCAAATTC AGATCGCATG GCCTGACGGA AGAGGCTCCG 
CCTCCCCCAT CTCGCAAGGG CCTAAAGAAG CCCACATCGT CGGCGGTTGC CGCTTCACCC 
GCTTTAATGG CAGTCCCGTC TTCACTGAAG CGACCATCGC CGCCACCCAG GGATTTGTCC 
ACCGACAAGA AGGCCAAGAT CGACATCAAC AACGTCGCGG GACATGTGGA GAGGCCCGGA 
GCCGCCAAGC TGATAGCGCC CAAAAAGATT CAAACCCTGG TGGAGACCAA CCTGTTCTCG 
GACGCCCTGG CCGCCTCCAT CGAACCCAAA AAGGTGGTGA AGCGCAAGCG TACGTCTGCA 
ACATCGCCCA CGGACAAGGA CGCGCCACTG GCGCCGCTTA AGTTCTACCA GGATACTCTG 
GACGAGTCGA AGAGCGAGGA GAAGTCGGAC GACAGCAGCA AGGAAAACGA TGATGCGCGC 
TCCTCGTCAC CTGGCAAGGA CACGGATGCC GATGACGACG ACATTCCCCT TAAGAGGGTC 
AAGGAGGACA TCGAGCAAAA GGTGCAGAAG GCGAAGGCTG CGGCAGGCGG AGAAGGCGGA 
GAAGCGGGTA ACACCTCTGA CAACGCTGAA GATCTTCCCG AGGAGCCCAA GAAGCCTGGA 
CCTGGCTGTG GACCCGATGG ACCACCGGGA GTGCTCACGC TGCACCGCCG CAAGGGCCCG 
AAAAAGAAGC TAACCTGGCA GTCAGAGGAA AAGCTCACCC AGATACGATA CTTCGAGGTC 
GATCGCTCAG AGCGCGTAAA TGTGATGAAG CAGACCTTCC TGGAAATGAA GAACATGGAG 
CGATTCAGCG AGCGGGACGC GCTGACCATT GCTCGGCGTG GATTCGACGA TACCATGGAG 
CCGCAAATGG AGTGGCGGCC ACTACTCGAA GTCGATAATG TGCCCGACCA CCCGAACGGA 
AACCTCTCCA AGCAGCGCCA GGTGCAGATG GACAGGGAGG CCACCACTCT AAGAGCCCTG 
TACTTCTCGC CCGACATGAT TCCCGACAGT CCTGCGGAGG CGGAACTGGA ACCGCATTTT 
GCTCATGACA TTCCCGTGAT ACCCATGGAT GACCTGACGG GGAATCCGGA TGCTGTCAAC 
GACTACAGTG CCTTGGCGTG GCCGGAGCCC AAGAAATACG CGCCTAGCTC GGGAGTCGGA 
GCAAATATCA GCGGATCGGA CATGGGCTTC AATGACAATA TGTTGACCGG ACTGGGAGCT 
GGACCGAACC TAATGCAAAA TCCATTCGAT CCGTTCATGG GAGGCATGCC TGCTCCCACG 
GGAATGCCAC CCGGACCAAA CATGATGCCA AATCAAGTGC CCAACGGAAC GCCGCAAATC 
TGGCAGAACC AAATGGGTCC GGTAGGTGGG CCGCCCGGTG TACCGCCCAT GATGAACGGA 
CCCGGTGGCC CCGGAATGGA TATGAGCAAT ATGAACATGA ACAACATGCC ACCGCAAAAC 
TTTATGGGCA ATCAGTTTGG AAGTCCAGTG TCTCCATTCG GAAGCGGCCC ACCACCTGGA 
TTCAATCAGT ACCCGCCCAT GATGATGAAT GGACCAGGTC CGGGACCGGT AATGGGACCC 
AATGGTCCAG TGATGGGACC CAACGGCCCC ATGATGAACG GCCCACCCAA TGGACCCATG 
ATGAACGGGC CGCCCAATGG AATGGGGCCA GGACTGATGA TGGGCGGACC TAGGAATAAC 
AACAACAACA ACAACAGGAA CAAAAACAAC GGCGGCGGCA TCTGGCGCAG CGGGGGCAAC 
AACTTCCAGG AGAACTCCGG CGGCAACTGG CGCTCAGCAG GATCTGGCCC CAATCGAGGA 
GGTGGAGGAG GCAATGGCAA CACCGGCGTC TGCAAGCAGT TCATGCGAGG ACACTGCAAC 
ATGGGCAAGA ACTGCAAGTA TGTGCATCCG CAGAAGAAGC GCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNUTS ( NM_001014455.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014455.1 Drosophila melanogaster PNUTS, transcript variant B (PNUTS), mRNA.

Price & Availability↑

CloneID	OFa13410
Clone ID Related Accession (Same CDS sequence)	NM_001014455.2 , NM_001014454.2
Accession Version	NM_001014455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PNUTS, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001014455.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTCGTA TAGTTCCACT GCAATTGCTG AGGTGCCTGC GGGTGCTTTT GGACAATAAT 
GGTGGAATTT TAAGTGCAGT GGAGGTGAAA CGCATTTCGG GTTTGATGGC CAAGTATTCA 
AAAAAACTCG TCTCGAAATG TGTGTATGTA CAAATTCTGA AATCCACAAA GACGGAACTA 
TTGGGCGACT TTATGGCGGT GGGCGGTTGG TCGCTGGTCT ACACCTGGTT AAATGATGCC 
ATCAGGGCCA TGAACTGGCC GCTGGTACAG GAGATCCTGG AACTGCTGCT CCTTTCTCCC 
GTGGATGTCA ATCGCCTCAA GATAAACTCA GCACCTATTC TGGTCAAGGG GCTGTGCAAG 
GATGGCGGCA ACGAAGGTGT ACGCATTCTA GCCAAGCGTT TGGTAGAGCA GTGGCTGAAG 
ATTGTGACGG AAAACACAAG CATGACGATC CAAGGTGCTG CGCCACATAT TGCAGCCACT 
ACTGCCTCTC AGGTTCCTGC ATCCGTTTCT GGAGCTGGCT CCGCTTCAGT TCCGGACTCT 
GCCTCTTCTT CAGACAGCAC TGATAGCGCC GGAGCACCTG TGACGTATAC CATCACCTCG 
GCGGCCAACA GTAGCAATTC CAACAGCATT ACCATCAAGT GGAAGCCTTT GTTGGACAAC 
CCAGATGGTG GCGACGAAAC GGATTCTGGC ACACAATCGA AAGACAACGA GGTGGAACAT 
CTGGAAAAGG GCTCCTCCGC AACCCACTTA TTAGCAGATG CTAGTCTGGG GAAAAAGTCC 
GGGTTAGATG ATAGTAGCTC CGCGGCGGAG GTTAAGTCTG GGGAGGACGT GGATAATAAG 
AAGCATAAGA GCTCAAAGAG CGACAAAAGT AAACGGTCCG AGAAGGAGCG CGAGAAGGAT 
AGAAAGAAGG AAAGTTCAAG TCATAGGTCC TCCTCATCTA GCCATAAGTC GTCCTCTTCC 
TCGTCATCCT CCTCTTCGAA GAGCAGTTCT TCCAAGAGCT CCTCATCTTC TTCCTCTTCC 
CATCGCAGTT CTAGCGATAA ATACCGCGAT AAAGACAAGG CTCGCTCAAG TTCCGGCTCC 
TCCAGCTCGA GCAAGGACCG GGAGCGCAGC AGCGGTTCGT CTTCTTCTTC CTCGAATAAA 
CACAAGTCCT CAAAGTCCTC CTCGTCGTCG TCGTCGTCGT CGTCGAGCAG CTCTGGTTCT 
AGTTCGTCCA AAGATCGCAA GTCGTCGTCG TCAACTTCGT CATCATCCTC ATCCTCGAGG 
CAGGACAAGG ACAAGGACAA CAAGCTAATG CCTCCGCCAG CTGTGGCAGC ATCAAATGCT 
GCAGGTACAT CTCCTACAGC TTCGGCAAAG GAGAGCGACG CACAGAAGCC CAGATCGATT 
CCTATCATGT CCCGCAAGGC CTCCATTTCG ATAGAAATTC GCCGGGACAC CGAAAAGACG 
GCCACTGTAA AGACGTACCA GTCCAAATTC AGATCGCATG GCCTGACGGA AGAGGCTCCG 
CCTCCCCCAT CTCGCAAGGG CCTAAAGAAG CCCACATCGT CGGCGGTTGC CGCTTCACCC 
GCTTTAATGG CAGTCCCGTC TTCACTGAAG CGACCATCGC CGCCACCCAG GGATTTGTCC 
ACCGACAAGA AGGCCAAGAT CGACATCAAC AACGTCGCGG GACATGTGGA GAGGCCCGGA 
GCCGCCAAGC TGATAGCGCC CAAAAAGAGT AAGCAACAGA TGCTAGAATC ACTATTAATG 
CTCTTCTCTA TTTTATTCCA CAGCCCAAGA ACTTTGGTGG CTTTTGCTTT TGTTTTCGAT 
CGGAGTCGAA TGTCGAACTC CGCCGTATTA TTTGTTGATC TCCATTCCAT TCTCAAGGTG 
TTTAATTTTG CTTGGGGCGG GTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014455.1
CDS	322..2208
Translation	MPRIVPLQLLRCLRVLLDNNGGILSAVEVKRISGLMAKYSKKLVSKCVYVQILKSTKTELLGDFMAVGGWSLVYTWLNDAIRAMNWPLVQEILELLLLSPVDVNRLKINSAPILVKGLCKDGGNEGVRILAKRLVEQWLKIVTENTSMTIQGAAPHIAATTASQVPASVSGAGSASVPDSASSSDSTDSAGAPVTYTITSAANSSNSNSITIKWKPLLDNPDGGDETDSGTQSKDNEVEHLEKGSSATHLLADASLGKKSGLDDSSSAAEVKSGEDVDNKKHKSSKSDKSKRSEKEREKDRKKESSSHRSSSSSHKSSSSSSSSSSKSSSSKSSSSSSSSHRSSSDKYRDKDKARSSSGSSSSSKDRERSSGSSSSSSNKHKSSKSSSSSSSSSSSSSGSSSSKDRKSSSSTSSSSSSSRQDKDKDNKLMPPPAVAASNAAGTSPTASAKESDAQKPRSIPIMSRKASISIEIRRDTEKTATVKTYQSKFRSHGLTEEAPPPPSRKGLKKPTSSAVAASPALMAVPSSLKRPSPPPRDLSTDKKAKIDINNVAGHVERPGAAKLIAPKKSKQQMLESLLMLFSILFHSPRTLVAFAFVFDRSRMSNSAVLFVDLHSILKVFNFAWGGS

Target ORF information:

RefSeq Version	NM_001014455.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PNUTS, transcript variant B (PNUTS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014455.2

ORF Insert Sequence:

ATGCCTCGTA TAGTTCCACT GCAATTGCTG AGGTGCCTGC GGGTGCTTTT GGACAATAAT 
GGTGGAATTT TAAGTGCAGT GGAGGTGAAA CGCATTTCGG GTTTGATGGC CAAGTATTCA 
AAAAAACTCG TCTCGAAATG TGTGTATGTA CAAATTCTGA AATCCACAAA GACGGAACTA 
TTGGGCGACT TTATGGCGGT GGGCGGTTGG TCGCTGGTCT ACACCTGGTT AAATGATGCC 
ATCAGGGCCA TGAACTGGCC GCTGGTACAG GAGATCCTGG AACTGCTGCT CCTTTCTCCC 
GTGGATGTCA ATCGCCTCAA GATAAACTCA GCACCTATTC TGGTCAAGGG GCTGTGCAAG 
GATGGCGGCA ACGAAGGTGT ACGCATTCTA GCCAAGCGTT TGGTAGAGCA GTGGCTGAAG 
ATTGTGACGG AAAACACAAG CATGACGATC CAAGGTGCTG CGCCACATAT TGCAGCCACT 
ACTGCCTCTC AGGTTCCTGC ATCCGTTTCT GGAGCTGGCT CCGCTTCAGT TCCGGACTCT 
GCCTCTTCTT CAGACAGCAC TGATAGCGCC GGAGCACCTG TGACGTATAC CATCACCTCG 
GCGGCCAACA GTAGCAATTC CAACAGCATT ACCATCAAGT GGAAGCCTTT GTTGGACAAC 
CCAGATGGTG GCGACGAAAC GGATTCTGGC ACACAATCGA AAGACAACGA GGTGGAACAT 
CTGGAAAAGG GCTCCTCCGC AACCCACTTA TTAGCAGATG CTAGTCTGGG GAAAAAGTCC 
GGGTTAGATG ATAGTAGCTC CGCGGCGGAG GTTAAGTCTG GGGAGGACGT GGATAATAAG 
AAGCATAAGA GCTCAAAGAG CGACAAAAGT AAACGGTCCG AGAAGGAGCG CGAGAAGGAT 
AGAAAGAAGG AAAGTTCAAG TCATAGGTCC TCCTCATCTA GCCATAAGTC GTCCTCTTCC 
TCGTCATCCT CCTCTTCGAA GAGCAGTTCT TCCAAGAGCT CCTCATCTTC TTCCTCTTCC 
CATCGCAGTT CTAGCGATAA ATACCGCGAT AAAGACAAGG CTCGCTCAAG TTCCGGCTCC 
TCCAGCTCGA GCAAGGACCG GGAGCGCAGC AGCGGTTCGT CTTCTTCTTC CTCGAATAAA 
CACAAGTCCT CAAAGTCCTC CTCGTCGTCG TCGTCGTCGT CGTCGAGCAG CTCTGGTTCT 
AGTTCGTCCA AAGATCGCAA GTCGTCGTCG TCAACTTCGT CATCATCCTC ATCCTCGAGG 
CAGGACAAGG ACAAGGACAA CAAGCTAATG CCTCCGCCAG CTGTGGCAGC ATCAAATGCT 
GCAGGTACAT CTCCTACAGC TTCGGCAAAG GAGAGCGACG CACAGAAGCC CAGATCGATT 
CCTATCATGT CCCGCAAGGC CTCCATTTCG ATAGAAATTC GCCGGGACAC CGAAAAGACG 
GCCACTGTAA AGACGTACCA GTCCAAATTC AGATCGCATG GCCTGACGGA AGAGGCTCCG 
CCTCCCCCAT CTCGCAAGGG CCTAAAGAAG CCCACATCGT CGGCGGTTGC CGCTTCACCC 
GCTTTAATGG CAGTCCCGTC TTCACTGAAG CGACCATCGC CGCCACCCAG GGATTTGTCC 
ACCGACAAGA AGGCCAAGAT CGACATCAAC AACGTCGCGG GACATGTGGA GAGGCCCGGA 
GCCGCCAAGC TGATAGCGCC CAAAAAGAGT AAGCAACAGA TGCTAGAATC ACTATTAATG 
CTCTTCTCTA TTTTATTCCA CAGCCCAAGA ACTTTGGTGG CTTTTGCTTT TGTTTTCGAT 
CGGAGTCGAA TGTCGAACTC CGCCGTATTA TTTGTTGATC TCCATTCCAT TCTCAAGGTG 
TTTAATTTTG CTTGGGGCGG GTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNUTS ( NM_001014454.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014454.1 Drosophila melanogaster PNUTS, transcript variant A (PNUTS), mRNA.

Price & Availability↑

CloneID	OFa13410
Clone ID Related Accession (Same CDS sequence)	NM_001014455.2 , NM_001014454.2
Accession Version	NM_001014454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PNUTS, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001014454.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTCGTA TAGTTCCACT GCAATTGCTG AGGTGCCTGC GGGTGCTTTT GGACAATAAT 
GGTGGAATTT TAAGTGCAGT GGAGGTGAAA CGCATTTCGG GTTTGATGGC CAAGTATTCA 
AAAAAACTCG TCTCGAAATG TGTGTATGTA CAAATTCTGA AATCCACAAA GACGGAACTA 
TTGGGCGACT TTATGGCGGT GGGCGGTTGG TCGCTGGTCT ACACCTGGTT AAATGATGCC 
ATCAGGGCCA TGAACTGGCC GCTGGTACAG GAGATCCTGG AACTGCTGCT CCTTTCTCCC 
GTGGATGTCA ATCGCCTCAA GATAAACTCA GCACCTATTC TGGTCAAGGG GCTGTGCAAG 
GATGGCGGCA ACGAAGGTGT ACGCATTCTA GCCAAGCGTT TGGTAGAGCA GTGGCTGAAG 
ATTGTGACGG AAAACACAAG CATGACGATC CAAGGTGCTG CGCCACATAT TGCAGCCACT 
ACTGCCTCTC AGGTTCCTGC ATCCGTTTCT GGAGCTGGCT CCGCTTCAGT TCCGGACTCT 
GCCTCTTCTT CAGACAGCAC TGATAGCGCC GGAGCACCTG TGACGTATAC CATCACCTCG 
GCGGCCAACA GTAGCAATTC CAACAGCATT ACCATCAAGT GGAAGCCTTT GTTGGACAAC 
CCAGATGGTG GCGACGAAAC GGATTCTGGC ACACAATCGA AAGACAACGA GGTGGAACAT 
CTGGAAAAGG GCTCCTCCGC AACCCACTTA TTAGCAGATG CTAGTCTGGG GAAAAAGTCC 
GGGTTAGATG ATAGTAGCTC CGCGGCGGAG GTTAAGTCTG GGGAGGACGT GGATAATAAG 
AAGCATAAGA GCTCAAAGAG CGACAAAAGT AAACGGTCCG AGAAGGAGCG CGAGAAGGAT 
AGAAAGAAGG AAAGTTCAAG TCATAGGTCC TCCTCATCTA GCCATAAGTC GTCCTCTTCC 
TCGTCATCCT CCTCTTCGAA GAGCAGTTCT TCCAAGAGCT CCTCATCTTC TTCCTCTTCC 
CATCGCAGTT CTAGCGATAA ATACCGCGAT AAAGACAAGG CTCGCTCAAG TTCCGGCTCC 
TCCAGCTCGA GCAAGGACCG GGAGCGCAGC AGCGGTTCGT CTTCTTCTTC CTCGAATAAA 
CACAAGTCCT CAAAGTCCTC CTCGTCGTCG TCGTCGTCGT CGTCGAGCAG CTCTGGTTCT 
AGTTCGTCCA AAGATCGCAA GTCGTCGTCG TCAACTTCGT CATCATCCTC ATCCTCGAGG 
CAGGACAAGG ACAAGGACAA CAAGCTAATG CCTCCGCCAG CTGTGGCAGC ATCAAATGCT 
GCAGGTACAT CTCCTACAGC TTCGGCAAAG GAGAGCGACG CACAGAAGCC CAGATCGATT 
CCTATCATGT CCCGCAAGGC CTCCATTTCG ATAGAAATTC GCCGGGACAC CGAAAAGACG 
GCCACTGTAA AGACGTACCA GTCCAAATTC AGATCGCATG GCCTGACGGA AGAGGCTCCG 
CCTCCCCCAT CTCGCAAGGG CCTAAAGAAG CCCACATCGT CGGCGGTTGC CGCTTCACCC 
GCTTTAATGG CAGTCCCGTC TTCACTGAAG CGACCATCGC CGCCACCCAG GGATTTGTCC 
ACCGACAAGA AGGCCAAGAT CGACATCAAC AACGTCGCGG GACATGTGGA GAGGCCCGGA 
GCCGCCAAGC TGATAGCGCC CAAAAAGAGT AAGCAACAGA TGCTAGAATC ACTATTAATG 
CTCTTCTCTA TTTTATTCCA CAGCCCAAGA ACTTTGGTGG CTTTTGCTTT TGTTTTCGAT 
CGGAGTCGAA TGTCGAACTC CGCCGTATTA TTTGTTGATC TCCATTCCAT TCTCAAGGTG 
TTTAATTTTG CTTGGGGCGG GTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014454.1
CDS	414..2300
Translation	MPRIVPLQLLRCLRVLLDNNGGILSAVEVKRISGLMAKYSKKLVSKCVYVQILKSTKTELLGDFMAVGGWSLVYTWLNDAIRAMNWPLVQEILELLLLSPVDVNRLKINSAPILVKGLCKDGGNEGVRILAKRLVEQWLKIVTENTSMTIQGAAPHIAATTASQVPASVSGAGSASVPDSASSSDSTDSAGAPVTYTITSAANSSNSNSITIKWKPLLDNPDGGDETDSGTQSKDNEVEHLEKGSSATHLLADASLGKKSGLDDSSSAAEVKSGEDVDNKKHKSSKSDKSKRSEKEREKDRKKESSSHRSSSSSHKSSSSSSSSSSKSSSSKSSSSSSSSHRSSSDKYRDKDKARSSSGSSSSSKDRERSSGSSSSSSNKHKSSKSSSSSSSSSSSSSGSSSSKDRKSSSSTSSSSSSSRQDKDKDNKLMPPPAVAASNAAGTSPTASAKESDAQKPRSIPIMSRKASISIEIRRDTEKTATVKTYQSKFRSHGLTEEAPPPPSRKGLKKPTSSAVAASPALMAVPSSLKRPSPPPRDLSTDKKAKIDINNVAGHVERPGAAKLIAPKKSKQQMLESLLMLFSILFHSPRTLVAFAFVFDRSRMSNSAVLFVDLHSILKVFNFAWGGS

Target ORF information:

RefSeq Version	NM_001014454.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PNUTS, transcript variant A (PNUTS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014454.2

ORF Insert Sequence:

ATGCCTCGTA TAGTTCCACT GCAATTGCTG AGGTGCCTGC GGGTGCTTTT GGACAATAAT 
GGTGGAATTT TAAGTGCAGT GGAGGTGAAA CGCATTTCGG GTTTGATGGC CAAGTATTCA 
AAAAAACTCG TCTCGAAATG TGTGTATGTA CAAATTCTGA AATCCACAAA GACGGAACTA 
TTGGGCGACT TTATGGCGGT GGGCGGTTGG TCGCTGGTCT ACACCTGGTT AAATGATGCC 
ATCAGGGCCA TGAACTGGCC GCTGGTACAG GAGATCCTGG AACTGCTGCT CCTTTCTCCC 
GTGGATGTCA ATCGCCTCAA GATAAACTCA GCACCTATTC TGGTCAAGGG GCTGTGCAAG 
GATGGCGGCA ACGAAGGTGT ACGCATTCTA GCCAAGCGTT TGGTAGAGCA GTGGCTGAAG 
ATTGTGACGG AAAACACAAG CATGACGATC CAAGGTGCTG CGCCACATAT TGCAGCCACT 
ACTGCCTCTC AGGTTCCTGC ATCCGTTTCT GGAGCTGGCT CCGCTTCAGT TCCGGACTCT 
GCCTCTTCTT CAGACAGCAC TGATAGCGCC GGAGCACCTG TGACGTATAC CATCACCTCG 
GCGGCCAACA GTAGCAATTC CAACAGCATT ACCATCAAGT GGAAGCCTTT GTTGGACAAC 
CCAGATGGTG GCGACGAAAC GGATTCTGGC ACACAATCGA AAGACAACGA GGTGGAACAT 
CTGGAAAAGG GCTCCTCCGC AACCCACTTA TTAGCAGATG CTAGTCTGGG GAAAAAGTCC 
GGGTTAGATG ATAGTAGCTC CGCGGCGGAG GTTAAGTCTG GGGAGGACGT GGATAATAAG 
AAGCATAAGA GCTCAAAGAG CGACAAAAGT AAACGGTCCG AGAAGGAGCG CGAGAAGGAT 
AGAAAGAAGG AAAGTTCAAG TCATAGGTCC TCCTCATCTA GCCATAAGTC GTCCTCTTCC 
TCGTCATCCT CCTCTTCGAA GAGCAGTTCT TCCAAGAGCT CCTCATCTTC TTCCTCTTCC 
CATCGCAGTT CTAGCGATAA ATACCGCGAT AAAGACAAGG CTCGCTCAAG TTCCGGCTCC 
TCCAGCTCGA GCAAGGACCG GGAGCGCAGC AGCGGTTCGT CTTCTTCTTC CTCGAATAAA 
CACAAGTCCT CAAAGTCCTC CTCGTCGTCG TCGTCGTCGT CGTCGAGCAG CTCTGGTTCT 
AGTTCGTCCA AAGATCGCAA GTCGTCGTCG TCAACTTCGT CATCATCCTC ATCCTCGAGG 
CAGGACAAGG ACAAGGACAA CAAGCTAATG CCTCCGCCAG CTGTGGCAGC ATCAAATGCT 
GCAGGTACAT CTCCTACAGC TTCGGCAAAG GAGAGCGACG CACAGAAGCC CAGATCGATT 
CCTATCATGT CCCGCAAGGC CTCCATTTCG ATAGAAATTC GCCGGGACAC CGAAAAGACG 
GCCACTGTAA AGACGTACCA GTCCAAATTC AGATCGCATG GCCTGACGGA AGAGGCTCCG 
CCTCCCCCAT CTCGCAAGGG CCTAAAGAAG CCCACATCGT CGGCGGTTGC CGCTTCACCC 
GCTTTAATGG CAGTCCCGTC TTCACTGAAG CGACCATCGC CGCCACCCAG GGATTTGTCC 
ACCGACAAGA AGGCCAAGAT CGACATCAAC AACGTCGCGG GACATGTGGA GAGGCCCGGA 
GCCGCCAAGC TGATAGCGCC CAAAAAGAGT AAGCAACAGA TGCTAGAATC ACTATTAATG 
CTCTTCTCTA TTTTATTCCA CAGCCCAAGA ACTTTGGTGG CTTTTGCTTT TGTTTTCGAT 
CGGAGTCGAA TGTCGAACTC CGCCGTATTA TTTGTTGATC TCCATTCCAT TCTCAAGGTG 
TTTAATTTTG CTTGGGGCGG GTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PNUTS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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