ush cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ush
Entrez Gene ID	33225
Full Name	u-shaped
Synonyms	CG2762,Dmel\CG2762,FOG,USH,Ush,ZFPM2,dFOG,l(2)19,u-sh,ushaped
General protein information	Preferred Names u-shaped Names CG2762 gene product from transcript CG2762-RE CG2762-PA CG2762-PB CG2762-PC CG2762-PD CG2762-PE U-shaped drosophila friend of GATA friend of GATA ush-PA ush-PB ush-PC ush-PD ush-PE ushaped
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21D1-21E2\|2-0.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272902.1	NP_001259831.1	u-shaped, isoform E
NM_001272899.1	NP_001259828.1	u-shaped, isoform B
NM_057432.3	NP_476780.1	u-shaped, isoform A
NM_001272900.1	NP_001259829.1	u-shaped, isoform C
NM_001272901.1	NP_001259830.1	u-shaped, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ush	NP_001259829.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009066	XP_319815.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It has been shown that Dorsal repressed Ush expression levels to promote hemocytes differentiation, whereas Cactus maintained Ush levels to block differentiation.
Title: The Friend of GATA Transcriptional Co-Regulator, U-Shaped, Is a Downstream Antagonist of Dorsal-Driven Prohemocyte Differentiation in Drosophila.

Mechanistic insights into hh transcriptional regulation in hematopoietic progenitor niche cells, demonstrate the requirement of the Srp, Su(H) and Ush proteins in the control of niche cell differentiation and blood cell precursor maintenance.
Title: Serpent, suppressor of hairless and U-shaped are crucial regulators of hedgehog niche expression and prohemocyte maintenance during Drosophila larval hematopoiesis.

This study uncovers complex transcriptional interactions between the pnr isoforms and the cofactor Ush that may be important for regulation of proneural expression and thorax patterning.
Title: Transcriptional interactions between the pannier isoforms and the cofactor U-shaped during neural development in Drosophila.

a conserved microRNA miR-8 and its target, USH, regulate body size in Drosophila. miR-8 null flies are smaller in size and defective in insulin signaling in fat body that is the fly counterpart of liver and adipose tissue.
Title: Conserved MicroRNA miR-8/miR-200 and its target USH/FOG2 control growth by regulating PI3K.

The Friend of GATA protein U-shaped is an important regulator of lymph gland prohemocyte potency and differentiation.
Title: Upregulation of the Drosophila Friend of GATA gene U-shaped by JAK/STAT signaling maintains lymph gland prohemocyte potency.

The following ush gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ush cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13357	NM_057432.3 Latest version!	Drosophila melanogaster u-shaped (ush), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15869	NM_001272902.1 Latest version!	Drosophila melanogaster u-shaped (ush), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15869	NM_001272899.1 Latest version!	Drosophila melanogaster u-shaped (ush), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15870	NM_001272901.1 Latest version!	Drosophila melanogaster u-shaped (ush), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15871	NM_001272900.1 Latest version!	Drosophila melanogaster u-shaped (ush), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ush ( NM_057432.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476780.1 Drosophila melanogaster u-shaped (ush), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13357
Clone ID Related Accession (Same CDS sequence)	NM_057432.3
Accession Version	NM_057432.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	u-shaped, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057432.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTAGCA GCAATACAAG AGGTGATTGT TCCGATACCG CAGAAGAGAT GACCGTCGAC 
AGCAGAGATT CCAAAGATCT GAGTGCTCAG GATATAGGCG AGCAGAAGCA GCAGCAAATG 
GAGGACCAGT TGGAGGATCA GTTGAACGAT TCCAGAGACC CCCAAAACAA CAATAACAAT 
ATCGATGACG ACGCTGATGA AGATGCTGAG TTCGAGGAGC CAGAAAAGGC CAATCCCCAA 
CAGGATCAGG ATTTGGGAGA GACAGAGATG GAACAGGAAC ACGATCTTCA GCAGGAGGAT 
CTTCAGCAGG AACTGCCTGC AAACAGCCCA AGCACTCCGC CCAGGAGTCC CAGCTCTCCC 
CAGCTGATTC CCAAGCTGGA ACAGCCCGCT ACGCCGCCCT CGGAGCCAGA AGCCTCTCCG 
TGCCCCTCAC CCTCACCATG TCCCACGCCC AAGTACCCCA AAGTGCGTCT GAATGCGCTC 
CTGGCCTCAG ATCCTGCCCT TAAGCCAGAT GCCAAGGAGC TGACCCTGCC GGACTCTCGT 
CTCCTGGCTC CTCCGCCGCT CGTCAAGCCG GACACGCAAG CGCAACCGGA GGTAGCAGAA 
CCTCTGCTAA AACCAGCCAG ATTTATGTGC CTGCCCTGTG GGATTGCATT CAGTTCGCCC 
TCAACTCTGG AGGCCCATCA AGCCTACTAT TGCTCTCATA GAATTAAGGA CACGGACGAG 
GCTGGCAGTG ATAAGTCAGG GGCAGGAGGA TCGGGAGCCA CAGCTGGCGA TGCAGCAGGG 
TTGACCGGAG GCTCCACCGA GCCACCAGCC AAAATGGCCA GGACAGGAAA GCAATATGGC 
TGCACCCAGT GCTCCTACAG CGCGGATAAG AAGGTATCCC TAAATCGCCA CATGCGCATG 
CACCAGACTT CGCCAGCAGC TCCCACACTG GCCGGCTTGC CAAGTCTTCT CCAGAACGGA 
ATAGCGCCGC CGGGAGTTAC GCCCAATCCG ATGGAGGATA GCTCTAGTCA ACAAACCGAT 
CGCTACTGCA GCCACTGTGA TATCCGGTTC AACAACATCA AGACCTATCG AGCGCACAAA 
CAACACTACT GCAGTTCGCG GCGACCAGAG GGACAGCTCA CGCCCAAGCC AGACGCCTCT 
CCGGGAGCAG GTTCCGGACC GGGTTCCGCA GGCGGATCCA TAGGAGTATC AGCCCAGGCG 
GCTACGCCCG GCAAGCTGAG TCCGCAGGCA AGGAACAAGA CTCCGACTCC CGCAATGGTC 
GCCGTCGCAG CTGCAGCAGC GGCTGCCGCT GCCTCCCTCC AGGCCACGCC GCATTCGCAT 
CCACCTTTCC TGGCACTGCC CACCCACCCG ATCATAATTG TGCCCTGCTC GCTGATCCGT 
GCAGCTAGCT TTATTCCAGG CCCACTGCCC ACGCCGAACT CGGGAATCGT GAATCCTGAA 
ACCACCTGTT TTACCGTGGA CAACGGAACG ATTAAACCAC TGGCCACTGC CCTAGTTGGC 
GCCACCTTAG AACCCGAACG CCCTTCAGCT CCATCATCTG CTGCTGAGGC CACAGAAGCC 
AAGAGTAGTC CTCCGGAGCC CAAGCGAAAA GAGGCAGGGT TAACTAGAGA ATCTGCGCCT 
CTCGATCTCT CGCTGCGTCG ATCGCCAATC ACCCTGAATT CACTGAGCTT GCGTCAGCGC 
CAACTGCGGA ATGCTCTCTT AGATGTGGAA GAAGTACTCC TTGCCGGCGT AGGAACCGGC 
AAGGAGAACG TTGAGACTCC GAGAGGAGGT GGAAGTGTGA CTCCCGAGCA AATCGTTTGC 
GCTCCCTCCC TGCCGAGCAG TCCTTCGATG AGTCCCTCGC CCAAAAGACG AGCCATCAGT 
CCACGCAGTT CCGGGGCTGG TAGCGCCTCC TCCATGTCAC CACCTGGTCT CAATGTGGCT 
GTTCCCCATC TGCTCGACAT GCGCTCCATG CTACCTGCGG ACTTTGGACT CTCCGAGTCG 
CTGTTGGCCA AAACCAACCC GGAACTGGCT CTGAAACTGG CGGCGGCAGC GGCCGCAGCT 
GCTGTGGCGG GCAGTAGTGG AGCAGCAGCC TTCCCGCCCG CTTCTCTTCC TGCCCAAACT 
AGTAGTGGGA ATCCGGGTAG TGGAGGATCG GCAGGTGGCG CCCAGCAGCC TCAGATCTAT 
GTGAAAAAGG GCGTGTCCAA GTGCATGGAG TGCAATATTG TGTTTTGCAA GTATGAGAAC 
TACTTGGCAC ACAAACAGCA TTATTGCTCG GCAAGAAGCC AAGAAGGTGC GAGTGAAGTG 
GATGTCAAGT CGGCCGTTTC ACCATCCATC GCTGGAGCAG GAGGACTGGG AGCCGGAGCT 
GCAGAGGCTG CTTCGTCGGT GGAAACCACA CCGGTGGCAT ATCAACAGTT GATCTGCGCC 
GCCTGCGGAA TTAAGTACAC TTCCCTGGAC AACCTGCGAG CCCATCAGAA CTACTATTGC 
CCCAAGGGAG GAGCAGTTGC TGCCCCTGCC GCGACGCCCA CGGATCCTGG CCAACTGGGC 
ATGCCAAAGG AGAAGTGCGG AAAGTGCAAG ACATTGCATG AAATTGGTCT GCCTTGTCCA 
CCACCCGTTG CGAATCCTCT CGCAGCGCCG ACAGTCAATC CGCAACCCGC CACCAATAGT 
CTGAACAAGT GTCCTGTTTG CGGGGTAGTG AGTCCCACAG CAGCACTGGC CAAGAAACAC 
ATGGAGATGC ACGGAACAGT AAAGGCATAC CGCTGCAGCA TCTGCCAGTA CAAGGGGAAC 
ACTCTTCGCG GCATGCGCAC CCACATTCGA ACCCATTTCG ACAAGAAGAC TAGCGACGTA 
AACGAGGAGC TATACATGAC CTGCATCTTT GAAGAGGATG CTAGCGCGCT GAGTCAGGAA 
CTGGTTACTC CAACAGGGGC ATCTACCACA ACTGGACACG ATTCCATGGA TCATCCCTCG 
CAGATGTTCA ACTGTGATTA CTGCAACTAT GTCTCCACCT ACAAGGGCAA TGTGCTGCGC 
CACATGAAGC TGATGCATCC CCATGTGGCC ATCAACTCTC CCTCCATTTC CCCCGACACC 
AGAGATCAAG ATGTGACTTC CAACCCCACT ACCAACCAAC ACTCGAATTC CGATGTATCC 
AACGGTGAAG CACCCAGTTT CCACATTAAA TCGGAGCCTC TGGATCCACC ACCGACTGTG 
AATCTGGTGC ACGAGAACAA CAACTCCCCG ATCGCCACGC CACATATTAA GGCTGAGCCA 
ATCGAAGTCG GAGCGGATGC TGCGCCCGGC GGGCTGGTTC CGCCGATGAC TTCTCCGCTG 
GGAAACAGCA GCAGCGTGGC TGCCGCAGCA GCTGCCGCCG CCGAGGTGAT GAAGAAGTAC 
TGCTCAACCT GTGACATATC CTTCAATTAC GTGAAGACCT ACCTGGCTCA CAAGCAGTTC 
TACTGCAAAA ACAAGCCCAT TCGCCCCGAG GCCAGCGACA GCCCCAGTCC GAATCACCTG 
GGCGGAGGCG TTGCCGTGGG CCTGGGCATT GGCGGCCTGG TCGGCGGACA CGGCCAGCAG 
AAGAACAAGG AAAACCTGCA GGAGGCGGCC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476780.1
CDS	573..4148
Translation	MLSSNTRGDCSDTAEEMTVDSRDSKDLSAQDIGEQKQQQMEDQLEDQLNDSRDPQNNNNNIDDDADEDAEFEEPEKANPQQDQDLGETEMEQEHDLQQEDLQQELPANSPSTPPRSPSSPQLIPKLEQPATPPSEPEASPCPSPSPCPTPKYPKVRLNALLASDPALKPDAKELTLPDSRLLAPPPLVKPDTQAQPEVAEPLLKPARFMCLPCGIAFSSPSTLEAHQAYYCSHRIKDTDEAGSDKSGAGGSGATAGDAAGLTGGSTEPPAKMARTGKQYGCTQCSYSADKKVSLNRHMRMHQTSPAAPTLAGLPSLLQNGIAPPGVTPNPMEDSSSQQTDRYCSHCDIRFNNIKTYRAHKQHYCSSRRPEGQLTPKPDASPGAGSGPGSAGGSIGVSAQAATPGKLSPQARNKTPTPAMVAVAAAAAAAAASLQATPHSHPPFLALPTHPIIIVPCSLIRAASFIPGPLPTPNSGIVNPETTCFTVDNGTIKPLATALVGATLEPERPSAPSSAAEATEAKSSPPEPKRKEAGLTRESAPLDLSLRRSPITLNSLSLRQRQLRNALLDVEEVLLAGVGTGKENVETPRGGGSVTPEQIVCAPSLPSSPSMSPSPKRRAISPRSSGAGSASSMSPPGLNVAVPHLLDMRSMLPADFGLSESLLAKTNPELALKLAAAAAAAAVAGSSGAAAFPPASLPAQTSSGNPGSGGSAGGAQQPQIYVKKGVSKCMECNIVFCKYENYLAHKQHYCSARSQEGASEVDVKSAVSPSIAGAGGLGAGAAEAASSVETTPVAYQQLICAACGIKYTSLDNLRAHQNYYCPKGGAVAAPAATPTDPGQLGMPKEKCGKCKTLHEIGLPCPPPVANPLAAPTVNPQPATNSLNKCPVCGVVSPTAALAKKHMEMHGTVKAYRCSICQYKGNTLRGMRTHIRTHFDKKTSDVNEELYMTCIFEEDASALSQELVTPTGASTTTGHDSMDHPSQMFNCDYCNYVSTYKGNVLRHMKLMHPHVAINSPSISPDTRDQDVTSNPTTNQHSNSDVSNGEAPSFHIKSEPLDPPPTVNLVHENNNSPIATPHIKAEPIEVGADAAPGGLVPPMTSPLGNSSSVAAAAAAAAEVMKKYCSTCDISFNYVKTYLAHKQFYCKNKPIRPEASDSPSPNHLGGGVAVGLGIGGLVGGHGQQKNKENLQEAAI

Target ORF information:

RefSeq Version	NM_057432.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster u-shaped (ush), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057432.3

ORF Insert Sequence:

ATGCTTAGCA GCAATACAAG AGGTGATTGT TCCGATACCG CAGAAGAGAT GACCGTCGAC 
AGCAGAGATT CCAAAGATCT GAGTGCTCAG GATATAGGCG AGCAGAAGCA GCAGCAAATG 
GAGGACCAGT TGGAGGATCA GTTGAACGAT TCCAGAGACC CCCAAAACAA CAATAACAAT 
ATCGATGACG ACGCTGATGA AGATGCTGAG TTCGAGGAGC CAGAAAAGGC CAATCCCCAA 
CAGGATCAGG ATTTGGGAGA GACAGAGATG GAACAGGAAC ACGATCTTCA GCAGGAGGAT 
CTTCAGCAGG AACTGCCTGC AAACAGCCCA AGCACTCCGC CCAGGAGTCC CAGCTCTCCC 
CAGCTGATTC CCAAGCTGGA ACAGCCCGCT ACGCCGCCCT CGGAGCCAGA AGCCTCTCCG 
TGCCCCTCAC CCTCACCATG TCCCACGCCC AAGTACCCCA AAGTGCGTCT GAATGCGCTC 
CTGGCCTCAG ATCCTGCCCT TAAGCCAGAT GCCAAGGAGC TGACCCTGCC GGACTCTCGT 
CTCCTGGCTC CTCCGCCGCT CGTCAAGCCG GACACGCAAG CGCAACCGGA GGTAGCAGAA 
CCTCTGCTAA AACCAGCCAG ATTTATGTGC CTGCCCTGTG GGATTGCATT CAGTTCGCCC 
TCAACTCTGG AGGCCCATCA AGCCTACTAT TGCTCTCATA GAATTAAGGA CACGGACGAG 
GCTGGCAGTG ATAAGTCAGG GGCAGGAGGA TCGGGAGCCA CAGCTGGCGA TGCAGCAGGG 
TTGACCGGAG GCTCCACCGA GCCACCAGCC AAAATGGCCA GGACAGGAAA GCAATATGGC 
TGCACCCAGT GCTCCTACAG CGCGGATAAG AAGGTATCCC TAAATCGCCA CATGCGCATG 
CACCAGACTT CGCCAGCAGC TCCCACACTG GCCGGCTTGC CAAGTCTTCT CCAGAACGGA 
ATAGCGCCGC CGGGAGTTAC GCCCAATCCG ATGGAGGATA GCTCTAGTCA ACAAACCGAT 
CGCTACTGCA GCCACTGTGA TATCCGGTTC AACAACATCA AGACCTATCG AGCGCACAAA 
CAACACTACT GCAGTTCGCG GCGACCAGAG GGACAGCTCA CGCCCAAGCC AGACGCCTCT 
CCGGGAGCAG GTTCCGGACC GGGTTCCGCA GGCGGATCCA TAGGAGTATC AGCCCAGGCG 
GCTACGCCCG GCAAGCTGAG TCCGCAGGCA AGGAACAAGA CTCCGACTCC CGCAATGGTC 
GCCGTCGCAG CTGCAGCAGC GGCTGCCGCT GCCTCCCTCC AGGCCACGCC GCATTCGCAT 
CCACCTTTCC TGGCACTGCC CACCCACCCG ATCATAATTG TGCCCTGCTC GCTGATCCGT 
GCAGCTAGCT TTATTCCAGG CCCACTGCCC ACGCCGAACT CGGGAATCGT GAATCCTGAA 
ACCACCTGTT TTACCGTGGA CAACGGAACG ATTAAACCAC TGGCCACTGC CCTAGTTGGC 
GCCACCTTAG AACCCGAACG CCCTTCAGCT CCATCATCTG CTGCTGAGGC CACAGAAGCC 
AAGAGTAGTC CTCCGGAGCC CAAGCGAAAA GAGGCAGGGT TAACTAGAGA ATCTGCGCCT 
CTCGATCTCT CGCTGCGTCG ATCGCCAATC ACCCTGAATT CACTGAGCTT GCGTCAGCGC 
CAACTGCGGA ATGCTCTCTT AGATGTGGAA GAAGTACTCC TTGCCGGCGT AGGAACCGGC 
AAGGAGAACG TTGAGACTCC GAGAGGAGGT GGAAGTGTGA CTCCCGAGCA AATCGTTTGC 
GCTCCCTCCC TGCCGAGCAG TCCTTCGATG AGTCCCTCGC CCAAAAGACG AGCCATCAGT 
CCACGCAGTT CCGGGGCTGG TAGCGCCTCC TCCATGTCAC CACCTGGTCT CAATGTGGCT 
GTTCCCCATC TGCTCGACAT GCGCTCCATG CTACCTGCGG ACTTTGGACT CTCCGAGTCG 
CTGTTGGCCA AAACCAACCC GGAACTGGCT CTGAAACTGG CGGCGGCAGC GGCCGCAGCT 
GCTGTGGCGG GCAGTAGTGG AGCAGCAGCC TTCCCGCCCG CTTCTCTTCC TGCCCAAACT 
AGTAGTGGGA ATCCGGGTAG TGGAGGATCG GCAGGTGGCG CCCAGCAGCC TCAGATCTAT 
GTGAAAAAGG GCGTGTCCAA GTGCATGGAG TGCAATATTG TGTTTTGCAA GTATGAGAAC 
TACTTGGCAC ACAAACAGCA TTATTGCTCG GCAAGAAGCC AAGAAGGTGC GAGTGAAGTG 
GATGTCAAGT CGGCCGTTTC ACCATCCATC GCTGGAGCAG GAGGACTGGG AGCCGGAGCT 
GCAGAGGCTG CTTCGTCGGT GGAAACCACA CCGGTGGCAT ATCAACAGTT GATCTGCGCC 
GCCTGCGGAA TTAAGTACAC TTCCCTGGAC AACCTGCGAG CCCATCAGAA CTACTATTGC 
CCCAAGGGAG GAGCAGTTGC TGCCCCTGCC GCGACGCCCA CGGATCCTGG CCAACTGGGC 
ATGCCAAAGG AGAAGTGCGG AAAGTGCAAG ACATTGCATG AAATTGGTCT GCCTTGTCCA 
CCACCCGTTG CGAATCCTCT CGCAGCGCCG ACAGTCAATC CGCAACCCGC CACCAATAGT 
CTGAACAAGT GTCCTGTTTG CGGGGTAGTG AGTCCCACAG CAGCACTGGC CAAGAAACAC 
ATGGAGATGC ACGGAACAGT AAAGGCATAC CGCTGCAGCA TCTGCCAGTA CAAGGGGAAC 
ACTCTTCGCG GCATGCGCAC CCACATTCGA ACCCATTTCG ACAAGAAGAC TAGCGACGTA 
AACGAGGAGC TATACATGAC CTGCATCTTT GAAGAGGATG CTAGCGCGCT GAGTCAGGAA 
CTGGTTACTC CAACAGGGGC ATCTACCACA ACTGGACACG ATTCCATGGA TCATCCCTCG 
CAGATGTTCA ACTGTGATTA CTGCAACTAT GTCTCCACCT ACAAGGGCAA TGTGCTGCGC 
CACATGAAGC TGATGCATCC CCATGTGGCC ATCAACTCTC CCTCCATTTC CCCCGACACC 
AGAGATCAAG ATGTGACTTC CAACCCCACT ACCAACCAAC ACTCGAATTC CGATGTATCC 
AACGGTGAAG CACCCAGTTT CCACATTAAA TCGGAGCCTC TGGATCCACC ACCGACTGTG 
AATCTGGTGC ACGAGAACAA CAACTCCCCG ATCGCCACGC CACATATTAA GGCTGAGCCA 
ATCGAAGTCG GAGCGGATGC TGCGCCCGGC GGGCTGGTTC CGCCGATGAC TTCTCCGCTG 
GGAAACAGCA GCAGCGTGGC TGCCGCAGCA GCTGCCGCCG CCGAGGTGAT GAAGAAGTAC 
TGCTCAACCT GTGACATATC CTTCAATTAC GTGAAGACCT ACCTGGCTCA CAAGCAGTTC 
TACTGCAAAA ACAAGCCCAT TCGCCCCGAG GCCAGCGACA GCCCCAGTCC GAATCACCTG 
GGCGGAGGCG TTGCCGTGGG CCTGGGCATT GGCGGCCTGG TCGGCGGACA CGGCCAGCAG 
AAGAACAAGG AAAACCTGCA GGAGGCGGCC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush ( NM_001272902.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259831.1 Drosophila melanogaster u-shaped (ush), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa15869
Clone ID Related Accession (Same CDS sequence)	NM_001272902.1 , NM_001272899.1
Accession Version	NM_001272902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	u-shaped, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCACGAC GCAAACAGTC CAACCCGAAG CCGTTGAATA AAGGTGATTG TTCCGATACC 
GCAGAAGAGA TGACCGTCGA CAGCAGAGAT TCCAAAGATC TGAGTGCTCA GGATATAGGC 
GAGCAGAAGC AGCAGCAAAT GGAGGACCAG TTGGAGGATC AGTTGAACGA TTCCAGAGAC 
CCCCAAAACA ACAATAACAA TATCGATGAC GACGCTGATG AAGATGCTGA GTTCGAGGAG 
CCAGAAAAGG CCAATCCCCA ACAGGATCAG GATTTGGGAG AGACAGAGAT GGAACAGGAA 
CACGATCTTC AGCAGGAGGA TCTTCAGCAG GAACTGCCTG CAAACAGCCC AAGCACTCCG 
CCCAGGAGTC CCAGCTCTCC CCAGCTGATT CCCAAGCTGG AACAGCCCGC TACGCCGCCC 
TCGGAGCCAG AAGCCTCTCC GTGCCCCTCA CCCTCACCAT GTCCCACGCC CAAGTACCCC 
AAAGTGCGTC TGAATGCGCT CCTGGCCTCA GATCCTGCCC TTAAGCCAGA TGCCAAGGAG 
CTGACCCTGC CGGACTCTCG TCTCCTGGCT CCTCCGCCGC TCGTCAAGCC GGACACGCAA 
GCGCAACCGG AGGTAGCAGA ACCTCTGCTA AAACCAGCCA GATTTATGTG CCTGCCCTGT 
GGGATTGCAT TCAGTTCGCC CTCAACTCTG GAGGCCCATC AAGCCTACTA TTGCTCTCAT 
AGAATTAAGG ACACGGACGA GGCTGGCAGT GATAAGTCAG GGGCAGGAGG ATCGGGAGCC 
ACAGCTGGCG ATGCAGCAGG GTTGACCGGA GGCTCCACCG AGCCACCAGC CAAAATGGCC 
AGGACAGGAA AGCAATATGG CTGCACCCAG TGCTCCTACA GCGCGGATAA GAAGGTATCC 
CTAAATCGCC ACATGCGCAT GCACCAGACT TCGCCAGCAG CTCCCACACT GGCCGGCTTG 
CCAAGTCTTC TCCAGAACGG AATAGCGCCG CCGGGAGTTA CGCCCAATCC GATGGAGGAT 
AGCTCTAGTC AACAAACCGA TCGCTACTGC AGCCACTGTG ATATCCGGTT CAACAACATC 
AAGACCTATC GAGCGCACAA ACAACACTAC TGCAGTTCGC GGCGACCAGA GGGACAGCTC 
ACGCCCAAGC CAGACGCCTC TCCGGGAGCA GGTTCCGGAC CGGGTTCCGC AGGCGGATCC 
ATAGGAGTAT CAGCCCAGGC GGCTACGCCC GGCAAGCTGA GTCCGCAGGC AAGGAACAAG 
ACTCCGACTC CCGCAATGGT CGCCGTCGCA GCTGCAGCAG CGGCTGCCGC TGCCTCCCTC 
CAGGCCACGC CGCATTCGCA TCCACCTTTC CTGGCACTGC CCACCCACCC GATCATAATT 
GTGCCCTGCT CGCTGATCCG TGCAGCTAGC TTTATTCCAG GCCCACTGCC CACGCCGAAC 
TCGGGAATCG TGAATCCTGA AACCACCTGT TTTACCGTGG ACAACGGAAC GATTAAACCA 
CTGGCCACTG CCCTAGTTGG CGCCACCTTA GAACCCGAAC GCCCTTCAGC TCCATCATCT 
GCTGCTGAGG CCACAGAAGC CAAGAGTAGT CCTCCGGAGC CCAAGCGAAA AGAGGCAGGG 
TTAACTAGAG AATCTGCGCC TCTCGATCTC TCGCTGCGTC GATCGCCAAT CACCCTGAAT 
TCACTGAGCT TGCGTCAGCG CCAACTGCGG AATGCTCTCT TAGATGTGGA AGAAGTACTC 
CTTGCCGGCG TAGGAACCGG CAAGGAGAAC GTTGAGACTC CGAGAGGAGG TGGAAGTGTG 
ACTCCCGAGC AAATCGTTTG CGCTCCCTCC CTGCCGAGCA GTCCTTCGAT GAGTCCCTCG 
CCCAAAAGAC GAGCCATCAG TCCACGCAGT TCCGGGGCTG GTAGCGCCTC CTCCATGTCA 
CCACCTGGTC TCAATGTGGC TGTTCCCCAT CTGCTCGACA TGCGCTCCAT GCTACCTGCG 
GACTTTGGAC TCTCCGAGTC GCTGTTGGCC AAAACCAACC CGGAACTGGC TCTGAAACTG 
GCGGCGGCAG CGGCCGCAGC TGCTGTGGCG GGCAGTAGTG GAGCAGCAGC CTTCCCGCCC 
GCTTCTCTTC CTGCCCAAAC TAGTAGTGGG AATCCGGGTA GTGGAGGATC GGCAGGTGGC 
GCCCAGCAGC CTCAGATCTA TGTGAAAAAG GGCGTGTCCA AGTGCATGGA GTGCAATATT 
GTGTTTTGCA AGTATGAGAA CTACTTGGCA CACAAACAGC ATTATTGCTC GGCAAGAAGC 
CAAGAAGGTG CGAGTGAAGT GGATGTCAAG TCGGCCGTTT CACCATCCAT CGCTGGAGCA 
GGAGGACTGG GAGCCGGAGC TGCAGAGGCT GCTTCGTCGG TGGAAACCAC ACCGGTGGCA 
TATCAACAGT TGATCTGCGC CGCCTGCGGA ATTAAGTACA CTTCCCTGGA CAACCTGCGA 
GCCCATCAGA ACTACTATTG CCCCAAGGGA GGAGCAGTTG CTGCCCCTGC CGCGACGCCC 
ACGGATCCTG GCCAACTGGG CATGCCAAAG GAGAAGTGCG GAAAGTGCAA GACATTGCAT 
GAAATTGGTC TGCCTTGTCC ACCACCCGTT GCGAATCCTC TCGCAGCGCC GACAGTCAAT 
CCGCAACCCG CCACCAATAG TCTGAACAAG TGTCCTGTTT GCGGGGTAGT GAGTCCCACA 
GCAGCACTGG CCAAGAAACA CATGGAGATG CACGGAACAG TAAAGGCATA CCGCTGCAGC 
ATCTGCCAGT ACAAGGGGAA CACTCTTCGC GGCATGCGCA CCCACATTCG AACCCATTTC 
GACAAGAAGA CTAGCGACGT AAACGAGGAG CTATACATGA CCTGCATCTT TGAAGAGGAT 
GCTAGCGCGC TGAGTCAGGA ACTGGTTACT CCAACAGGGG CATCTACCAC AACTGGACAC 
GATTCCATGG ATCATCCCTC GCAGATGTTC AACTGTGATT ACTGCAACTA TGTCTCCACC 
TACAAGGGCA ATGTGCTGCG CCACATGAAG CTGATGCATC CCCATGTGGC CATCAACTCT 
CCCTCCATTT CCCCCGACAC CAGAGATCAA GATGTGACTT CCAACCCCAC TACCAACCAA 
CACTCGAATT CCGATGTATC CAACGGTGAA GCACCCAGTT TCCACATTAA ATCGGAGCCT 
CTGGATCCAC CACCGACTGT GAATCTGGTG CACGAGAACA ACAACTCCCC GATCGCCACG 
CCACATATTA AGGCTGAGCC AATCGAAGTC GGAGCGGATG CTGCGCCCGG CGGGCTGGTT 
CCGCCGATGA CTTCTCCGCT GGGAAACAGC AGCAGCGTGG CTGCCGCAGC AGCTGCCGCC 
GCCGAGGTGA TGAAGAAGTA CTGCTCAACC TGTGACATAT CCTTCAATTA CGTGAAGACC 
TACCTGGCTC ACAAGCAGTT CTACTGCAAA AACAAGCCCA TTCGCCCCGA GGCCAGCGAC 
AGCCCCAGTC CGAATCACCT GGGCGGAGGC GTTGCCGTGG GCCTGGGCAT TGGCGGCCTG 
GTCGGCGGAC ACGGCCAGCA GAAGAACAAG GAAAACCTGC AGGAGGCGGC CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259831.1
CDS	1912..5508
Translation	MSRRKQSNPKPLNKGDCSDTAEEMTVDSRDSKDLSAQDIGEQKQQQMEDQLEDQLNDSRDPQNNNNNIDDDADEDAEFEEPEKANPQQDQDLGETEMEQEHDLQQEDLQQELPANSPSTPPRSPSSPQLIPKLEQPATPPSEPEASPCPSPSPCPTPKYPKVRLNALLASDPALKPDAKELTLPDSRLLAPPPLVKPDTQAQPEVAEPLLKPARFMCLPCGIAFSSPSTLEAHQAYYCSHRIKDTDEAGSDKSGAGGSGATAGDAAGLTGGSTEPPAKMARTGKQYGCTQCSYSADKKVSLNRHMRMHQTSPAAPTLAGLPSLLQNGIAPPGVTPNPMEDSSSQQTDRYCSHCDIRFNNIKTYRAHKQHYCSSRRPEGQLTPKPDASPGAGSGPGSAGGSIGVSAQAATPGKLSPQARNKTPTPAMVAVAAAAAAAAASLQATPHSHPPFLALPTHPIIIVPCSLIRAASFIPGPLPTPNSGIVNPETTCFTVDNGTIKPLATALVGATLEPERPSAPSSAAEATEAKSSPPEPKRKEAGLTRESAPLDLSLRRSPITLNSLSLRQRQLRNALLDVEEVLLAGVGTGKENVETPRGGGSVTPEQIVCAPSLPSSPSMSPSPKRRAISPRSSGAGSASSMSPPGLNVAVPHLLDMRSMLPADFGLSESLLAKTNPELALKLAAAAAAAAVAGSSGAAAFPPASLPAQTSSGNPGSGGSAGGAQQPQIYVKKGVSKCMECNIVFCKYENYLAHKQHYCSARSQEGASEVDVKSAVSPSIAGAGGLGAGAAEAASSVETTPVAYQQLICAACGIKYTSLDNLRAHQNYYCPKGGAVAAPAATPTDPGQLGMPKEKCGKCKTLHEIGLPCPPPVANPLAAPTVNPQPATNSLNKCPVCGVVSPTAALAKKHMEMHGTVKAYRCSICQYKGNTLRGMRTHIRTHFDKKTSDVNEELYMTCIFEEDASALSQELVTPTGASTTTGHDSMDHPSQMFNCDYCNYVSTYKGNVLRHMKLMHPHVAINSPSISPDTRDQDVTSNPTTNQHSNSDVSNGEAPSFHIKSEPLDPPPTVNLVHENNNSPIATPHIKAEPIEVGADAAPGGLVPPMTSPLGNSSSVAAAAAAAAEVMKKYCSTCDISFNYVKTYLAHKQFYCKNKPIRPEASDSPSPNHLGGGVAVGLGIGGLVGGHGQQKNKENLQEAAI

Target ORF information:

RefSeq Version	NM_001272902.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster u-shaped (ush), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272902.1

ORF Insert Sequence:

ATGTCACGAC GCAAACAGTC CAACCCGAAG CCGTTGAATA AAGGTGATTG TTCCGATACC 
GCAGAAGAGA TGACCGTCGA CAGCAGAGAT TCCAAAGATC TGAGTGCTCA GGATATAGGC 
GAGCAGAAGC AGCAGCAAAT GGAGGACCAG TTGGAGGATC AGTTGAACGA TTCCAGAGAC 
CCCCAAAACA ACAATAACAA TATCGATGAC GACGCTGATG AAGATGCTGA GTTCGAGGAG 
CCAGAAAAGG CCAATCCCCA ACAGGATCAG GATTTGGGAG AGACAGAGAT GGAACAGGAA 
CACGATCTTC AGCAGGAGGA TCTTCAGCAG GAACTGCCTG CAAACAGCCC AAGCACTCCG 
CCCAGGAGTC CCAGCTCTCC CCAGCTGATT CCCAAGCTGG AACAGCCCGC TACGCCGCCC 
TCGGAGCCAG AAGCCTCTCC GTGCCCCTCA CCCTCACCAT GTCCCACGCC CAAGTACCCC 
AAAGTGCGTC TGAATGCGCT CCTGGCCTCA GATCCTGCCC TTAAGCCAGA TGCCAAGGAG 
CTGACCCTGC CGGACTCTCG TCTCCTGGCT CCTCCGCCGC TCGTCAAGCC GGACACGCAA 
GCGCAACCGG AGGTAGCAGA ACCTCTGCTA AAACCAGCCA GATTTATGTG CCTGCCCTGT 
GGGATTGCAT TCAGTTCGCC CTCAACTCTG GAGGCCCATC AAGCCTACTA TTGCTCTCAT 
AGAATTAAGG ACACGGACGA GGCTGGCAGT GATAAGTCAG GGGCAGGAGG ATCGGGAGCC 
ACAGCTGGCG ATGCAGCAGG GTTGACCGGA GGCTCCACCG AGCCACCAGC CAAAATGGCC 
AGGACAGGAA AGCAATATGG CTGCACCCAG TGCTCCTACA GCGCGGATAA GAAGGTATCC 
CTAAATCGCC ACATGCGCAT GCACCAGACT TCGCCAGCAG CTCCCACACT GGCCGGCTTG 
CCAAGTCTTC TCCAGAACGG AATAGCGCCG CCGGGAGTTA CGCCCAATCC GATGGAGGAT 
AGCTCTAGTC AACAAACCGA TCGCTACTGC AGCCACTGTG ATATCCGGTT CAACAACATC 
AAGACCTATC GAGCGCACAA ACAACACTAC TGCAGTTCGC GGCGACCAGA GGGACAGCTC 
ACGCCCAAGC CAGACGCCTC TCCGGGAGCA GGTTCCGGAC CGGGTTCCGC AGGCGGATCC 
ATAGGAGTAT CAGCCCAGGC GGCTACGCCC GGCAAGCTGA GTCCGCAGGC AAGGAACAAG 
ACTCCGACTC CCGCAATGGT CGCCGTCGCA GCTGCAGCAG CGGCTGCCGC TGCCTCCCTC 
CAGGCCACGC CGCATTCGCA TCCACCTTTC CTGGCACTGC CCACCCACCC GATCATAATT 
GTGCCCTGCT CGCTGATCCG TGCAGCTAGC TTTATTCCAG GCCCACTGCC CACGCCGAAC 
TCGGGAATCG TGAATCCTGA AACCACCTGT TTTACCGTGG ACAACGGAAC GATTAAACCA 
CTGGCCACTG CCCTAGTTGG CGCCACCTTA GAACCCGAAC GCCCTTCAGC TCCATCATCT 
GCTGCTGAGG CCACAGAAGC CAAGAGTAGT CCTCCGGAGC CCAAGCGAAA AGAGGCAGGG 
TTAACTAGAG AATCTGCGCC TCTCGATCTC TCGCTGCGTC GATCGCCAAT CACCCTGAAT 
TCACTGAGCT TGCGTCAGCG CCAACTGCGG AATGCTCTCT TAGATGTGGA AGAAGTACTC 
CTTGCCGGCG TAGGAACCGG CAAGGAGAAC GTTGAGACTC CGAGAGGAGG TGGAAGTGTG 
ACTCCCGAGC AAATCGTTTG CGCTCCCTCC CTGCCGAGCA GTCCTTCGAT GAGTCCCTCG 
CCCAAAAGAC GAGCCATCAG TCCACGCAGT TCCGGGGCTG GTAGCGCCTC CTCCATGTCA 
CCACCTGGTC TCAATGTGGC TGTTCCCCAT CTGCTCGACA TGCGCTCCAT GCTACCTGCG 
GACTTTGGAC TCTCCGAGTC GCTGTTGGCC AAAACCAACC CGGAACTGGC TCTGAAACTG 
GCGGCGGCAG CGGCCGCAGC TGCTGTGGCG GGCAGTAGTG GAGCAGCAGC CTTCCCGCCC 
GCTTCTCTTC CTGCCCAAAC TAGTAGTGGG AATCCGGGTA GTGGAGGATC GGCAGGTGGC 
GCCCAGCAGC CTCAGATCTA TGTGAAAAAG GGCGTGTCCA AGTGCATGGA GTGCAATATT 
GTGTTTTGCA AGTATGAGAA CTACTTGGCA CACAAACAGC ATTATTGCTC GGCAAGAAGC 
CAAGAAGGTG CGAGTGAAGT GGATGTCAAG TCGGCCGTTT CACCATCCAT CGCTGGAGCA 
GGAGGACTGG GAGCCGGAGC TGCAGAGGCT GCTTCGTCGG TGGAAACCAC ACCGGTGGCA 
TATCAACAGT TGATCTGCGC CGCCTGCGGA ATTAAGTACA CTTCCCTGGA CAACCTGCGA 
GCCCATCAGA ACTACTATTG CCCCAAGGGA GGAGCAGTTG CTGCCCCTGC CGCGACGCCC 
ACGGATCCTG GCCAACTGGG CATGCCAAAG GAGAAGTGCG GAAAGTGCAA GACATTGCAT 
GAAATTGGTC TGCCTTGTCC ACCACCCGTT GCGAATCCTC TCGCAGCGCC GACAGTCAAT 
CCGCAACCCG CCACCAATAG TCTGAACAAG TGTCCTGTTT GCGGGGTAGT GAGTCCCACA 
GCAGCACTGG CCAAGAAACA CATGGAGATG CACGGAACAG TAAAGGCATA CCGCTGCAGC 
ATCTGCCAGT ACAAGGGGAA CACTCTTCGC GGCATGCGCA CCCACATTCG AACCCATTTC 
GACAAGAAGA CTAGCGACGT AAACGAGGAG CTATACATGA CCTGCATCTT TGAAGAGGAT 
GCTAGCGCGC TGAGTCAGGA ACTGGTTACT CCAACAGGGG CATCTACCAC AACTGGACAC 
GATTCCATGG ATCATCCCTC GCAGATGTTC AACTGTGATT ACTGCAACTA TGTCTCCACC 
TACAAGGGCA ATGTGCTGCG CCACATGAAG CTGATGCATC CCCATGTGGC CATCAACTCT 
CCCTCCATTT CCCCCGACAC CAGAGATCAA GATGTGACTT CCAACCCCAC TACCAACCAA 
CACTCGAATT CCGATGTATC CAACGGTGAA GCACCCAGTT TCCACATTAA ATCGGAGCCT 
CTGGATCCAC CACCGACTGT GAATCTGGTG CACGAGAACA ACAACTCCCC GATCGCCACG 
CCACATATTA AGGCTGAGCC AATCGAAGTC GGAGCGGATG CTGCGCCCGG CGGGCTGGTT 
CCGCCGATGA CTTCTCCGCT GGGAAACAGC AGCAGCGTGG CTGCCGCAGC AGCTGCCGCC 
GCCGAGGTGA TGAAGAAGTA CTGCTCAACC TGTGACATAT CCTTCAATTA CGTGAAGACC 
TACCTGGCTC ACAAGCAGTT CTACTGCAAA AACAAGCCCA TTCGCCCCGA GGCCAGCGAC 
AGCCCCAGTC CGAATCACCT GGGCGGAGGC GTTGCCGTGG GCCTGGGCAT TGGCGGCCTG 
GTCGGCGGAC ACGGCCAGCA GAAGAACAAG GAAAACCTGC AGGAGGCGGC CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush ( NM_001272899.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259828.1 Drosophila melanogaster u-shaped (ush), transcript variant B, mRNA.

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CloneID	OFa15869
Clone ID Related Accession (Same CDS sequence)	NM_001272902.1 , NM_001272899.1
Accession Version	NM_001272899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	u-shaped, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCACGAC GCAAACAGTC CAACCCGAAG CCGTTGAATA AAGGTGATTG TTCCGATACC 
GCAGAAGAGA TGACCGTCGA CAGCAGAGAT TCCAAAGATC TGAGTGCTCA GGATATAGGC 
GAGCAGAAGC AGCAGCAAAT GGAGGACCAG TTGGAGGATC AGTTGAACGA TTCCAGAGAC 
CCCCAAAACA ACAATAACAA TATCGATGAC GACGCTGATG AAGATGCTGA GTTCGAGGAG 
CCAGAAAAGG CCAATCCCCA ACAGGATCAG GATTTGGGAG AGACAGAGAT GGAACAGGAA 
CACGATCTTC AGCAGGAGGA TCTTCAGCAG GAACTGCCTG CAAACAGCCC AAGCACTCCG 
CCCAGGAGTC CCAGCTCTCC CCAGCTGATT CCCAAGCTGG AACAGCCCGC TACGCCGCCC 
TCGGAGCCAG AAGCCTCTCC GTGCCCCTCA CCCTCACCAT GTCCCACGCC CAAGTACCCC 
AAAGTGCGTC TGAATGCGCT CCTGGCCTCA GATCCTGCCC TTAAGCCAGA TGCCAAGGAG 
CTGACCCTGC CGGACTCTCG TCTCCTGGCT CCTCCGCCGC TCGTCAAGCC GGACACGCAA 
GCGCAACCGG AGGTAGCAGA ACCTCTGCTA AAACCAGCCA GATTTATGTG CCTGCCCTGT 
GGGATTGCAT TCAGTTCGCC CTCAACTCTG GAGGCCCATC AAGCCTACTA TTGCTCTCAT 
AGAATTAAGG ACACGGACGA GGCTGGCAGT GATAAGTCAG GGGCAGGAGG ATCGGGAGCC 
ACAGCTGGCG ATGCAGCAGG GTTGACCGGA GGCTCCACCG AGCCACCAGC CAAAATGGCC 
AGGACAGGAA AGCAATATGG CTGCACCCAG TGCTCCTACA GCGCGGATAA GAAGGTATCC 
CTAAATCGCC ACATGCGCAT GCACCAGACT TCGCCAGCAG CTCCCACACT GGCCGGCTTG 
CCAAGTCTTC TCCAGAACGG AATAGCGCCG CCGGGAGTTA CGCCCAATCC GATGGAGGAT 
AGCTCTAGTC AACAAACCGA TCGCTACTGC AGCCACTGTG ATATCCGGTT CAACAACATC 
AAGACCTATC GAGCGCACAA ACAACACTAC TGCAGTTCGC GGCGACCAGA GGGACAGCTC 
ACGCCCAAGC CAGACGCCTC TCCGGGAGCA GGTTCCGGAC CGGGTTCCGC AGGCGGATCC 
ATAGGAGTAT CAGCCCAGGC GGCTACGCCC GGCAAGCTGA GTCCGCAGGC AAGGAACAAG 
ACTCCGACTC CCGCAATGGT CGCCGTCGCA GCTGCAGCAG CGGCTGCCGC TGCCTCCCTC 
CAGGCCACGC CGCATTCGCA TCCACCTTTC CTGGCACTGC CCACCCACCC GATCATAATT 
GTGCCCTGCT CGCTGATCCG TGCAGCTAGC TTTATTCCAG GCCCACTGCC CACGCCGAAC 
TCGGGAATCG TGAATCCTGA AACCACCTGT TTTACCGTGG ACAACGGAAC GATTAAACCA 
CTGGCCACTG CCCTAGTTGG CGCCACCTTA GAACCCGAAC GCCCTTCAGC TCCATCATCT 
GCTGCTGAGG CCACAGAAGC CAAGAGTAGT CCTCCGGAGC CCAAGCGAAA AGAGGCAGGG 
TTAACTAGAG AATCTGCGCC TCTCGATCTC TCGCTGCGTC GATCGCCAAT CACCCTGAAT 
TCACTGAGCT TGCGTCAGCG CCAACTGCGG AATGCTCTCT TAGATGTGGA AGAAGTACTC 
CTTGCCGGCG TAGGAACCGG CAAGGAGAAC GTTGAGACTC CGAGAGGAGG TGGAAGTGTG 
ACTCCCGAGC AAATCGTTTG CGCTCCCTCC CTGCCGAGCA GTCCTTCGAT GAGTCCCTCG 
CCCAAAAGAC GAGCCATCAG TCCACGCAGT TCCGGGGCTG GTAGCGCCTC CTCCATGTCA 
CCACCTGGTC TCAATGTGGC TGTTCCCCAT CTGCTCGACA TGCGCTCCAT GCTACCTGCG 
GACTTTGGAC TCTCCGAGTC GCTGTTGGCC AAAACCAACC CGGAACTGGC TCTGAAACTG 
GCGGCGGCAG CGGCCGCAGC TGCTGTGGCG GGCAGTAGTG GAGCAGCAGC CTTCCCGCCC 
GCTTCTCTTC CTGCCCAAAC TAGTAGTGGG AATCCGGGTA GTGGAGGATC GGCAGGTGGC 
GCCCAGCAGC CTCAGATCTA TGTGAAAAAG GGCGTGTCCA AGTGCATGGA GTGCAATATT 
GTGTTTTGCA AGTATGAGAA CTACTTGGCA CACAAACAGC ATTATTGCTC GGCAAGAAGC 
CAAGAAGGTG CGAGTGAAGT GGATGTCAAG TCGGCCGTTT CACCATCCAT CGCTGGAGCA 
GGAGGACTGG GAGCCGGAGC TGCAGAGGCT GCTTCGTCGG TGGAAACCAC ACCGGTGGCA 
TATCAACAGT TGATCTGCGC CGCCTGCGGA ATTAAGTACA CTTCCCTGGA CAACCTGCGA 
GCCCATCAGA ACTACTATTG CCCCAAGGGA GGAGCAGTTG CTGCCCCTGC CGCGACGCCC 
ACGGATCCTG GCCAACTGGG CATGCCAAAG GAGAAGTGCG GAAAGTGCAA GACATTGCAT 
GAAATTGGTC TGCCTTGTCC ACCACCCGTT GCGAATCCTC TCGCAGCGCC GACAGTCAAT 
CCGCAACCCG CCACCAATAG TCTGAACAAG TGTCCTGTTT GCGGGGTAGT GAGTCCCACA 
GCAGCACTGG CCAAGAAACA CATGGAGATG CACGGAACAG TAAAGGCATA CCGCTGCAGC 
ATCTGCCAGT ACAAGGGGAA CACTCTTCGC GGCATGCGCA CCCACATTCG AACCCATTTC 
GACAAGAAGA CTAGCGACGT AAACGAGGAG CTATACATGA CCTGCATCTT TGAAGAGGAT 
GCTAGCGCGC TGAGTCAGGA ACTGGTTACT CCAACAGGGG CATCTACCAC AACTGGACAC 
GATTCCATGG ATCATCCCTC GCAGATGTTC AACTGTGATT ACTGCAACTA TGTCTCCACC 
TACAAGGGCA ATGTGCTGCG CCACATGAAG CTGATGCATC CCCATGTGGC CATCAACTCT 
CCCTCCATTT CCCCCGACAC CAGAGATCAA GATGTGACTT CCAACCCCAC TACCAACCAA 
CACTCGAATT CCGATGTATC CAACGGTGAA GCACCCAGTT TCCACATTAA ATCGGAGCCT 
CTGGATCCAC CACCGACTGT GAATCTGGTG CACGAGAACA ACAACTCCCC GATCGCCACG 
CCACATATTA AGGCTGAGCC AATCGAAGTC GGAGCGGATG CTGCGCCCGG CGGGCTGGTT 
CCGCCGATGA CTTCTCCGCT GGGAAACAGC AGCAGCGTGG CTGCCGCAGC AGCTGCCGCC 
GCCGAGGTGA TGAAGAAGTA CTGCTCAACC TGTGACATAT CCTTCAATTA CGTGAAGACC 
TACCTGGCTC ACAAGCAGTT CTACTGCAAA AACAAGCCCA TTCGCCCCGA GGCCAGCGAC 
AGCCCCAGTC CGAATCACCT GGGCGGAGGC GTTGCCGTGG GCCTGGGCAT TGGCGGCCTG 
GTCGGCGGAC ACGGCCAGCA GAAGAACAAG GAAAACCTGC AGGAGGCGGC CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259828.1
CDS	482..4078
Translation	MSRRKQSNPKPLNKGDCSDTAEEMTVDSRDSKDLSAQDIGEQKQQQMEDQLEDQLNDSRDPQNNNNNIDDDADEDAEFEEPEKANPQQDQDLGETEMEQEHDLQQEDLQQELPANSPSTPPRSPSSPQLIPKLEQPATPPSEPEASPCPSPSPCPTPKYPKVRLNALLASDPALKPDAKELTLPDSRLLAPPPLVKPDTQAQPEVAEPLLKPARFMCLPCGIAFSSPSTLEAHQAYYCSHRIKDTDEAGSDKSGAGGSGATAGDAAGLTGGSTEPPAKMARTGKQYGCTQCSYSADKKVSLNRHMRMHQTSPAAPTLAGLPSLLQNGIAPPGVTPNPMEDSSSQQTDRYCSHCDIRFNNIKTYRAHKQHYCSSRRPEGQLTPKPDASPGAGSGPGSAGGSIGVSAQAATPGKLSPQARNKTPTPAMVAVAAAAAAAAASLQATPHSHPPFLALPTHPIIIVPCSLIRAASFIPGPLPTPNSGIVNPETTCFTVDNGTIKPLATALVGATLEPERPSAPSSAAEATEAKSSPPEPKRKEAGLTRESAPLDLSLRRSPITLNSLSLRQRQLRNALLDVEEVLLAGVGTGKENVETPRGGGSVTPEQIVCAPSLPSSPSMSPSPKRRAISPRSSGAGSASSMSPPGLNVAVPHLLDMRSMLPADFGLSESLLAKTNPELALKLAAAAAAAAVAGSSGAAAFPPASLPAQTSSGNPGSGGSAGGAQQPQIYVKKGVSKCMECNIVFCKYENYLAHKQHYCSARSQEGASEVDVKSAVSPSIAGAGGLGAGAAEAASSVETTPVAYQQLICAACGIKYTSLDNLRAHQNYYCPKGGAVAAPAATPTDPGQLGMPKEKCGKCKTLHEIGLPCPPPVANPLAAPTVNPQPATNSLNKCPVCGVVSPTAALAKKHMEMHGTVKAYRCSICQYKGNTLRGMRTHIRTHFDKKTSDVNEELYMTCIFEEDASALSQELVTPTGASTTTGHDSMDHPSQMFNCDYCNYVSTYKGNVLRHMKLMHPHVAINSPSISPDTRDQDVTSNPTTNQHSNSDVSNGEAPSFHIKSEPLDPPPTVNLVHENNNSPIATPHIKAEPIEVGADAAPGGLVPPMTSPLGNSSSVAAAAAAAAEVMKKYCSTCDISFNYVKTYLAHKQFYCKNKPIRPEASDSPSPNHLGGGVAVGLGIGGLVGGHGQQKNKENLQEAAI

Target ORF information:

RefSeq Version	NM_001272899.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster u-shaped (ush), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272899.1

ORF Insert Sequence:

ATGTCACGAC GCAAACAGTC CAACCCGAAG CCGTTGAATA AAGGTGATTG TTCCGATACC 
GCAGAAGAGA TGACCGTCGA CAGCAGAGAT TCCAAAGATC TGAGTGCTCA GGATATAGGC 
GAGCAGAAGC AGCAGCAAAT GGAGGACCAG TTGGAGGATC AGTTGAACGA TTCCAGAGAC 
CCCCAAAACA ACAATAACAA TATCGATGAC GACGCTGATG AAGATGCTGA GTTCGAGGAG 
CCAGAAAAGG CCAATCCCCA ACAGGATCAG GATTTGGGAG AGACAGAGAT GGAACAGGAA 
CACGATCTTC AGCAGGAGGA TCTTCAGCAG GAACTGCCTG CAAACAGCCC AAGCACTCCG 
CCCAGGAGTC CCAGCTCTCC CCAGCTGATT CCCAAGCTGG AACAGCCCGC TACGCCGCCC 
TCGGAGCCAG AAGCCTCTCC GTGCCCCTCA CCCTCACCAT GTCCCACGCC CAAGTACCCC 
AAAGTGCGTC TGAATGCGCT CCTGGCCTCA GATCCTGCCC TTAAGCCAGA TGCCAAGGAG 
CTGACCCTGC CGGACTCTCG TCTCCTGGCT CCTCCGCCGC TCGTCAAGCC GGACACGCAA 
GCGCAACCGG AGGTAGCAGA ACCTCTGCTA AAACCAGCCA GATTTATGTG CCTGCCCTGT 
GGGATTGCAT TCAGTTCGCC CTCAACTCTG GAGGCCCATC AAGCCTACTA TTGCTCTCAT 
AGAATTAAGG ACACGGACGA GGCTGGCAGT GATAAGTCAG GGGCAGGAGG ATCGGGAGCC 
ACAGCTGGCG ATGCAGCAGG GTTGACCGGA GGCTCCACCG AGCCACCAGC CAAAATGGCC 
AGGACAGGAA AGCAATATGG CTGCACCCAG TGCTCCTACA GCGCGGATAA GAAGGTATCC 
CTAAATCGCC ACATGCGCAT GCACCAGACT TCGCCAGCAG CTCCCACACT GGCCGGCTTG 
CCAAGTCTTC TCCAGAACGG AATAGCGCCG CCGGGAGTTA CGCCCAATCC GATGGAGGAT 
AGCTCTAGTC AACAAACCGA TCGCTACTGC AGCCACTGTG ATATCCGGTT CAACAACATC 
AAGACCTATC GAGCGCACAA ACAACACTAC TGCAGTTCGC GGCGACCAGA GGGACAGCTC 
ACGCCCAAGC CAGACGCCTC TCCGGGAGCA GGTTCCGGAC CGGGTTCCGC AGGCGGATCC 
ATAGGAGTAT CAGCCCAGGC GGCTACGCCC GGCAAGCTGA GTCCGCAGGC AAGGAACAAG 
ACTCCGACTC CCGCAATGGT CGCCGTCGCA GCTGCAGCAG CGGCTGCCGC TGCCTCCCTC 
CAGGCCACGC CGCATTCGCA TCCACCTTTC CTGGCACTGC CCACCCACCC GATCATAATT 
GTGCCCTGCT CGCTGATCCG TGCAGCTAGC TTTATTCCAG GCCCACTGCC CACGCCGAAC 
TCGGGAATCG TGAATCCTGA AACCACCTGT TTTACCGTGG ACAACGGAAC GATTAAACCA 
CTGGCCACTG CCCTAGTTGG CGCCACCTTA GAACCCGAAC GCCCTTCAGC TCCATCATCT 
GCTGCTGAGG CCACAGAAGC CAAGAGTAGT CCTCCGGAGC CCAAGCGAAA AGAGGCAGGG 
TTAACTAGAG AATCTGCGCC TCTCGATCTC TCGCTGCGTC GATCGCCAAT CACCCTGAAT 
TCACTGAGCT TGCGTCAGCG CCAACTGCGG AATGCTCTCT TAGATGTGGA AGAAGTACTC 
CTTGCCGGCG TAGGAACCGG CAAGGAGAAC GTTGAGACTC CGAGAGGAGG TGGAAGTGTG 
ACTCCCGAGC AAATCGTTTG CGCTCCCTCC CTGCCGAGCA GTCCTTCGAT GAGTCCCTCG 
CCCAAAAGAC GAGCCATCAG TCCACGCAGT TCCGGGGCTG GTAGCGCCTC CTCCATGTCA 
CCACCTGGTC TCAATGTGGC TGTTCCCCAT CTGCTCGACA TGCGCTCCAT GCTACCTGCG 
GACTTTGGAC TCTCCGAGTC GCTGTTGGCC AAAACCAACC CGGAACTGGC TCTGAAACTG 
GCGGCGGCAG CGGCCGCAGC TGCTGTGGCG GGCAGTAGTG GAGCAGCAGC CTTCCCGCCC 
GCTTCTCTTC CTGCCCAAAC TAGTAGTGGG AATCCGGGTA GTGGAGGATC GGCAGGTGGC 
GCCCAGCAGC CTCAGATCTA TGTGAAAAAG GGCGTGTCCA AGTGCATGGA GTGCAATATT 
GTGTTTTGCA AGTATGAGAA CTACTTGGCA CACAAACAGC ATTATTGCTC GGCAAGAAGC 
CAAGAAGGTG CGAGTGAAGT GGATGTCAAG TCGGCCGTTT CACCATCCAT CGCTGGAGCA 
GGAGGACTGG GAGCCGGAGC TGCAGAGGCT GCTTCGTCGG TGGAAACCAC ACCGGTGGCA 
TATCAACAGT TGATCTGCGC CGCCTGCGGA ATTAAGTACA CTTCCCTGGA CAACCTGCGA 
GCCCATCAGA ACTACTATTG CCCCAAGGGA GGAGCAGTTG CTGCCCCTGC CGCGACGCCC 
ACGGATCCTG GCCAACTGGG CATGCCAAAG GAGAAGTGCG GAAAGTGCAA GACATTGCAT 
GAAATTGGTC TGCCTTGTCC ACCACCCGTT GCGAATCCTC TCGCAGCGCC GACAGTCAAT 
CCGCAACCCG CCACCAATAG TCTGAACAAG TGTCCTGTTT GCGGGGTAGT GAGTCCCACA 
GCAGCACTGG CCAAGAAACA CATGGAGATG CACGGAACAG TAAAGGCATA CCGCTGCAGC 
ATCTGCCAGT ACAAGGGGAA CACTCTTCGC GGCATGCGCA CCCACATTCG AACCCATTTC 
GACAAGAAGA CTAGCGACGT AAACGAGGAG CTATACATGA CCTGCATCTT TGAAGAGGAT 
GCTAGCGCGC TGAGTCAGGA ACTGGTTACT CCAACAGGGG CATCTACCAC AACTGGACAC 
GATTCCATGG ATCATCCCTC GCAGATGTTC AACTGTGATT ACTGCAACTA TGTCTCCACC 
TACAAGGGCA ATGTGCTGCG CCACATGAAG CTGATGCATC CCCATGTGGC CATCAACTCT 
CCCTCCATTT CCCCCGACAC CAGAGATCAA GATGTGACTT CCAACCCCAC TACCAACCAA 
CACTCGAATT CCGATGTATC CAACGGTGAA GCACCCAGTT TCCACATTAA ATCGGAGCCT 
CTGGATCCAC CACCGACTGT GAATCTGGTG CACGAGAACA ACAACTCCCC GATCGCCACG 
CCACATATTA AGGCTGAGCC AATCGAAGTC GGAGCGGATG CTGCGCCCGG CGGGCTGGTT 
CCGCCGATGA CTTCTCCGCT GGGAAACAGC AGCAGCGTGG CTGCCGCAGC AGCTGCCGCC 
GCCGAGGTGA TGAAGAAGTA CTGCTCAACC TGTGACATAT CCTTCAATTA CGTGAAGACC 
TACCTGGCTC ACAAGCAGTT CTACTGCAAA AACAAGCCCA TTCGCCCCGA GGCCAGCGAC 
AGCCCCAGTC CGAATCACCT GGGCGGAGGC GTTGCCGTGG GCCTGGGCAT TGGCGGCCTG 
GTCGGCGGAC ACGGCCAGCA GAAGAACAAG GAAAACCTGC AGGAGGCGGC CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush ( NM_001272901.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259830.1 Drosophila melanogaster u-shaped (ush), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa15870
Clone ID Related Accession (Same CDS sequence)	NM_001272901.1
Accession Version	NM_001272901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	u-shaped, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGTCG ACAGCAGAGA TTCCAAAGAT CTGAGTGCTC AGGATATAGG CGAGCAGAAG 
CAGCAGCAAA TGGAGGACCA GTTGGAGGAT CAGTTGAACG ATTCCAGAGA CCCCCAAAAC 
AACAATAACA ATATCGATGA CGACGCTGAT GAAGATGCTG AGTTCGAGGA GCCAGAAAAG 
GCCAATCCCC AACAGGATCA GGATTTGGGA GAGACAGAGA TGGAACAGGA ACACGATCTT 
CAGCAGGAGG ATCTTCAGCA GGAACTGCCT GCAAACAGCC CAAGCACTCC GCCCAGGAGT 
CCCAGCTCTC CCCAGCTGAT TCCCAAGCTG GAACAGCCCG CTACGCCGCC CTCGGAGCCA 
GAAGCCTCTC CGTGCCCCTC ACCCTCACCA TGTCCCACGC CCAAGTACCC CAAAGTGCGT 
CTGAATGCGC TCCTGGCCTC AGATCCTGCC CTTAAGCCAG ATGCCAAGGA GCTGACCCTG 
CCGGACTCTC GTCTCCTGGC TCCTCCGCCG CTCGTCAAGC CGGACACGCA AGCGCAACCG 
GAGGTAGCAG AACCTCTGCT AAAACCAGCC AGATTTATGT GCCTGCCCTG TGGGATTGCA 
TTCAGTTCGC CCTCAACTCT GGAGGCCCAT CAAGCCTACT ATTGCTCTCA TAGAATTAAG 
GACACGGACG AGGCTGGCAG TGATAAGTCA GGGGCAGGAG GATCGGGAGC CACAGCTGGC 
GATGCAGCAG GGTTGACCGG AGGCTCCACC GAGCCACCAG CCAAAATGGC CAGGACAGGA 
AAGCAATATG GCTGCACCCA GTGCTCCTAC AGCGCGGATA AGAAGGTATC CCTAAATCGC 
CACATGCGCA TGCACCAGAC TTCGCCAGCA GCTCCCACAC TGGCCGGCTT GCCAAGTCTT 
CTCCAGAACG GAATAGCGCC GCCGGGAGTT ACGCCCAATC CGATGGAGGA TAGCTCTAGT 
CAACAAACCG ATCGCTACTG CAGCCACTGT GATATCCGGT TCAACAACAT CAAGACCTAT 
CGAGCGCACA AACAACACTA CTGCAGTTCG CGGCGACCAG AGGGACAGCT CACGCCCAAG 
CCAGACGCCT CTCCGGGAGC AGGTTCCGGA CCGGGTTCCG CAGGCGGATC CATAGGAGTA 
TCAGCCCAGG CGGCTACGCC CGGCAAGCTG AGTCCGCAGG CAAGGAACAA GACTCCGACT 
CCCGCAATGG TCGCCGTCGC AGCTGCAGCA GCGGCTGCCG CTGCCTCCCT CCAGGCCACG 
CCGCATTCGC ATCCACCTTT CCTGGCACTG CCCACCCACC CGATCATAAT TGTGCCCTGC 
TCGCTGATCC GTGCAGCTAG CTTTATTCCA GGCCCACTGC CCACGCCGAA CTCGGGAATC 
GTGAATCCTG AAACCACCTG TTTTACCGTG GACAACGGAA CGATTAAACC ACTGGCCACT 
GCCCTAGTTG GCGCCACCTT AGAACCCGAA CGCCCTTCAG CTCCATCATC TGCTGCTGAG 
GCCACAGAAG CCAAGAGTAG TCCTCCGGAG CCCAAGCGAA AAGAGGCAGG GTTAACTAGA 
GAATCTGCGC CTCTCGATCT CTCGCTGCGT CGATCGCCAA TCACCCTGAA TTCACTGAGC 
TTGCGTCAGC GCCAACTGCG GAATGCTCTC TTAGATGTGG AAGAAGTACT CCTTGCCGGC 
GTAGGAACCG GCAAGGAGAA CGTTGAGACT CCGAGAGGAG GTGGAAGTGT GACTCCCGAG 
CAAATCGTTT GCGCTCCCTC CCTGCCGAGC AGTCCTTCGA TGAGTCCCTC GCCCAAAAGA 
CGAGCCATCA GTCCACGCAG TTCCGGGGCT GGTAGCGCCT CCTCCATGTC ACCACCTGGT 
CTCAATGTGG CTGTTCCCCA TCTGCTCGAC ATGCGCTCCA TGCTACCTGC GGACTTTGGA 
CTCTCCGAGT CGCTGTTGGC CAAAACCAAC CCGGAACTGG CTCTGAAACT GGCGGCGGCA 
GCGGCCGCAG CTGCTGTGGC GGGCAGTAGT GGAGCAGCAG CCTTCCCGCC CGCTTCTCTT 
CCTGCCCAAA CTAGTAGTGG GAATCCGGGT AGTGGAGGAT CGGCAGGTGG CGCCCAGCAG 
CCTCAGATCT ATGTGAAAAA GGGCGTGTCC AAGTGCATGG AGTGCAATAT TGTGTTTTGC 
AAGTATGAGA ACTACTTGGC ACACAAACAG CATTATTGCT CGGCAAGAAG CCAAGAAGGT 
GCGAGTGAAG TGGATGTCAA GTCGGCCGTT TCACCATCCA TCGCTGGAGC AGGAGGACTG 
GGAGCCGGAG CTGCAGAGGC TGCTTCGTCG GTGGAAACCA CACCGGTGGC ATATCAACAG 
TTGATCTGCG CCGCCTGCGG AATTAAGTAC ACTTCCCTGG ACAACCTGCG AGCCCATCAG 
AACTACTATT GCCCCAAGGG AGGAGCAGTT GCTGCCCCTG CCGCGACGCC CACGGATCCT 
GGCCAACTGG GCATGCCAAA GGAGAAGTGC GGAAAGTGCA AGACATTGCA TGAAATTGGT 
CTGCCTTGTC CACCACCCGT TGCGAATCCT CTCGCAGCGC CGACAGTCAA TCCGCAACCC 
GCCACCAATA GTCTGAACAA GTGTCCTGTT TGCGGGGTAG TGAGTCCCAC AGCAGCACTG 
GCCAAGAAAC ACATGGAGAT GCACGGAACA GTAAAGGCAT ACCGCTGCAG CATCTGCCAG 
TACAAGGGGA ACACTCTTCG CGGCATGCGC ACCCACATTC GAACCCATTT CGACAAGAAG 
ACTAGCGACG TAAACGAGGA GCTATACATG ACCTGCATCT TTGAAGAGGA TGCTAGCGCG 
CTGAGTCAGG AACTGGTTAC TCCAACAGGG GCATCTACCA CAACTGGACA CGATTCCATG 
GATCATCCCT CGCAGATGTT CAACTGTGAT TACTGCAACT ATGTCTCCAC CTACAAGGGC 
AATGTGCTGC GCCACATGAA GCTGATGCAT CCCCATGTGG CCATCAACTC TCCCTCCATT 
TCCCCCGACA CCAGAGATCA AGATGTGACT TCCAACCCCA CTACCAACCA ACACTCGAAT 
TCCGATGTAT CCAACGGTGA AGCACCCAGT TTCCACATTA AATCGGAGCC TCTGGATCCA 
CCACCGACTG TGAATCTGGT GCACGAGAAC AACAACTCCC CGATCGCCAC GCCACATATT 
AAGGCTGAGC CAATCGAAGT CGGAGCGGAT GCTGCGCCCG GCGGGCTGGT TCCGCCGATG 
ACTTCTCCGC TGGGAAACAG CAGCAGCGTG GCTGCCGCAG CAGCTGCCGC CGCCGAGGTG 
ATGAAGAAGT ACTGCTCAAC CTGTGACATA TCCTTCAATT ACGTGAAGAC CTACCTGGCT 
CACAAGCAGT TCTACTGCAA AAACAAGCCC ATTCGCCCCG AGGCCAGCGA CAGCCCCAGT 
CCGAATCACC TGGGCGGAGG CGTTGCCGTG GGCCTGGGCA TTGGCGGCCT GGTCGGCGGA 
CACGGCCAGC AGAAGAACAA GGAAAACCTG CAGGAGGCGG CCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259830.1
CDS	139..3666
Translation	MTVDSRDSKDLSAQDIGEQKQQQMEDQLEDQLNDSRDPQNNNNNIDDDADEDAEFEEPEKANPQQDQDLGETEMEQEHDLQQEDLQQELPANSPSTPPRSPSSPQLIPKLEQPATPPSEPEASPCPSPSPCPTPKYPKVRLNALLASDPALKPDAKELTLPDSRLLAPPPLVKPDTQAQPEVAEPLLKPARFMCLPCGIAFSSPSTLEAHQAYYCSHRIKDTDEAGSDKSGAGGSGATAGDAAGLTGGSTEPPAKMARTGKQYGCTQCSYSADKKVSLNRHMRMHQTSPAAPTLAGLPSLLQNGIAPPGVTPNPMEDSSSQQTDRYCSHCDIRFNNIKTYRAHKQHYCSSRRPEGQLTPKPDASPGAGSGPGSAGGSIGVSAQAATPGKLSPQARNKTPTPAMVAVAAAAAAAAASLQATPHSHPPFLALPTHPIIIVPCSLIRAASFIPGPLPTPNSGIVNPETTCFTVDNGTIKPLATALVGATLEPERPSAPSSAAEATEAKSSPPEPKRKEAGLTRESAPLDLSLRRSPITLNSLSLRQRQLRNALLDVEEVLLAGVGTGKENVETPRGGGSVTPEQIVCAPSLPSSPSMSPSPKRRAISPRSSGAGSASSMSPPGLNVAVPHLLDMRSMLPADFGLSESLLAKTNPELALKLAAAAAAAAVAGSSGAAAFPPASLPAQTSSGNPGSGGSAGGAQQPQIYVKKGVSKCMECNIVFCKYENYLAHKQHYCSARSQEGASEVDVKSAVSPSIAGAGGLGAGAAEAASSVETTPVAYQQLICAACGIKYTSLDNLRAHQNYYCPKGGAVAAPAATPTDPGQLGMPKEKCGKCKTLHEIGLPCPPPVANPLAAPTVNPQPATNSLNKCPVCGVVSPTAALAKKHMEMHGTVKAYRCSICQYKGNTLRGMRTHIRTHFDKKTSDVNEELYMTCIFEEDASALSQELVTPTGASTTTGHDSMDHPSQMFNCDYCNYVSTYKGNVLRHMKLMHPHVAINSPSISPDTRDQDVTSNPTTNQHSNSDVSNGEAPSFHIKSEPLDPPPTVNLVHENNNSPIATPHIKAEPIEVGADAAPGGLVPPMTSPLGNSSSVAAAAAAAAEVMKKYCSTCDISFNYVKTYLAHKQFYCKNKPIRPEASDSPSPNHLGGGVAVGLGIGGLVGGHGQQKNKENLQEAAI

Target ORF information:

RefSeq Version	NM_001272901.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster u-shaped (ush), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272901.1

ORF Insert Sequence:

ATGACCGTCG ACAGCAGAGA TTCCAAAGAT CTGAGTGCTC AGGATATAGG CGAGCAGAAG 
CAGCAGCAAA TGGAGGACCA GTTGGAGGAT CAGTTGAACG ATTCCAGAGA CCCCCAAAAC 
AACAATAACA ATATCGATGA CGACGCTGAT GAAGATGCTG AGTTCGAGGA GCCAGAAAAG 
GCCAATCCCC AACAGGATCA GGATTTGGGA GAGACAGAGA TGGAACAGGA ACACGATCTT 
CAGCAGGAGG ATCTTCAGCA GGAACTGCCT GCAAACAGCC CAAGCACTCC GCCCAGGAGT 
CCCAGCTCTC CCCAGCTGAT TCCCAAGCTG GAACAGCCCG CTACGCCGCC CTCGGAGCCA 
GAAGCCTCTC CGTGCCCCTC ACCCTCACCA TGTCCCACGC CCAAGTACCC CAAAGTGCGT 
CTGAATGCGC TCCTGGCCTC AGATCCTGCC CTTAAGCCAG ATGCCAAGGA GCTGACCCTG 
CCGGACTCTC GTCTCCTGGC TCCTCCGCCG CTCGTCAAGC CGGACACGCA AGCGCAACCG 
GAGGTAGCAG AACCTCTGCT AAAACCAGCC AGATTTATGT GCCTGCCCTG TGGGATTGCA 
TTCAGTTCGC CCTCAACTCT GGAGGCCCAT CAAGCCTACT ATTGCTCTCA TAGAATTAAG 
GACACGGACG AGGCTGGCAG TGATAAGTCA GGGGCAGGAG GATCGGGAGC CACAGCTGGC 
GATGCAGCAG GGTTGACCGG AGGCTCCACC GAGCCACCAG CCAAAATGGC CAGGACAGGA 
AAGCAATATG GCTGCACCCA GTGCTCCTAC AGCGCGGATA AGAAGGTATC CCTAAATCGC 
CACATGCGCA TGCACCAGAC TTCGCCAGCA GCTCCCACAC TGGCCGGCTT GCCAAGTCTT 
CTCCAGAACG GAATAGCGCC GCCGGGAGTT ACGCCCAATC CGATGGAGGA TAGCTCTAGT 
CAACAAACCG ATCGCTACTG CAGCCACTGT GATATCCGGT TCAACAACAT CAAGACCTAT 
CGAGCGCACA AACAACACTA CTGCAGTTCG CGGCGACCAG AGGGACAGCT CACGCCCAAG 
CCAGACGCCT CTCCGGGAGC AGGTTCCGGA CCGGGTTCCG CAGGCGGATC CATAGGAGTA 
TCAGCCCAGG CGGCTACGCC CGGCAAGCTG AGTCCGCAGG CAAGGAACAA GACTCCGACT 
CCCGCAATGG TCGCCGTCGC AGCTGCAGCA GCGGCTGCCG CTGCCTCCCT CCAGGCCACG 
CCGCATTCGC ATCCACCTTT CCTGGCACTG CCCACCCACC CGATCATAAT TGTGCCCTGC 
TCGCTGATCC GTGCAGCTAG CTTTATTCCA GGCCCACTGC CCACGCCGAA CTCGGGAATC 
GTGAATCCTG AAACCACCTG TTTTACCGTG GACAACGGAA CGATTAAACC ACTGGCCACT 
GCCCTAGTTG GCGCCACCTT AGAACCCGAA CGCCCTTCAG CTCCATCATC TGCTGCTGAG 
GCCACAGAAG CCAAGAGTAG TCCTCCGGAG CCCAAGCGAA AAGAGGCAGG GTTAACTAGA 
GAATCTGCGC CTCTCGATCT CTCGCTGCGT CGATCGCCAA TCACCCTGAA TTCACTGAGC 
TTGCGTCAGC GCCAACTGCG GAATGCTCTC TTAGATGTGG AAGAAGTACT CCTTGCCGGC 
GTAGGAACCG GCAAGGAGAA CGTTGAGACT CCGAGAGGAG GTGGAAGTGT GACTCCCGAG 
CAAATCGTTT GCGCTCCCTC CCTGCCGAGC AGTCCTTCGA TGAGTCCCTC GCCCAAAAGA 
CGAGCCATCA GTCCACGCAG TTCCGGGGCT GGTAGCGCCT CCTCCATGTC ACCACCTGGT 
CTCAATGTGG CTGTTCCCCA TCTGCTCGAC ATGCGCTCCA TGCTACCTGC GGACTTTGGA 
CTCTCCGAGT CGCTGTTGGC CAAAACCAAC CCGGAACTGG CTCTGAAACT GGCGGCGGCA 
GCGGCCGCAG CTGCTGTGGC GGGCAGTAGT GGAGCAGCAG CCTTCCCGCC CGCTTCTCTT 
CCTGCCCAAA CTAGTAGTGG GAATCCGGGT AGTGGAGGAT CGGCAGGTGG CGCCCAGCAG 
CCTCAGATCT ATGTGAAAAA GGGCGTGTCC AAGTGCATGG AGTGCAATAT TGTGTTTTGC 
AAGTATGAGA ACTACTTGGC ACACAAACAG CATTATTGCT CGGCAAGAAG CCAAGAAGGT 
GCGAGTGAAG TGGATGTCAA GTCGGCCGTT TCACCATCCA TCGCTGGAGC AGGAGGACTG 
GGAGCCGGAG CTGCAGAGGC TGCTTCGTCG GTGGAAACCA CACCGGTGGC ATATCAACAG 
TTGATCTGCG CCGCCTGCGG AATTAAGTAC ACTTCCCTGG ACAACCTGCG AGCCCATCAG 
AACTACTATT GCCCCAAGGG AGGAGCAGTT GCTGCCCCTG CCGCGACGCC CACGGATCCT 
GGCCAACTGG GCATGCCAAA GGAGAAGTGC GGAAAGTGCA AGACATTGCA TGAAATTGGT 
CTGCCTTGTC CACCACCCGT TGCGAATCCT CTCGCAGCGC CGACAGTCAA TCCGCAACCC 
GCCACCAATA GTCTGAACAA GTGTCCTGTT TGCGGGGTAG TGAGTCCCAC AGCAGCACTG 
GCCAAGAAAC ACATGGAGAT GCACGGAACA GTAAAGGCAT ACCGCTGCAG CATCTGCCAG 
TACAAGGGGA ACACTCTTCG CGGCATGCGC ACCCACATTC GAACCCATTT CGACAAGAAG 
ACTAGCGACG TAAACGAGGA GCTATACATG ACCTGCATCT TTGAAGAGGA TGCTAGCGCG 
CTGAGTCAGG AACTGGTTAC TCCAACAGGG GCATCTACCA CAACTGGACA CGATTCCATG 
GATCATCCCT CGCAGATGTT CAACTGTGAT TACTGCAACT ATGTCTCCAC CTACAAGGGC 
AATGTGCTGC GCCACATGAA GCTGATGCAT CCCCATGTGG CCATCAACTC TCCCTCCATT 
TCCCCCGACA CCAGAGATCA AGATGTGACT TCCAACCCCA CTACCAACCA ACACTCGAAT 
TCCGATGTAT CCAACGGTGA AGCACCCAGT TTCCACATTA AATCGGAGCC TCTGGATCCA 
CCACCGACTG TGAATCTGGT GCACGAGAAC AACAACTCCC CGATCGCCAC GCCACATATT 
AAGGCTGAGC CAATCGAAGT CGGAGCGGAT GCTGCGCCCG GCGGGCTGGT TCCGCCGATG 
ACTTCTCCGC TGGGAAACAG CAGCAGCGTG GCTGCCGCAG CAGCTGCCGC CGCCGAGGTG 
ATGAAGAAGT ACTGCTCAAC CTGTGACATA TCCTTCAATT ACGTGAAGAC CTACCTGGCT 
CACAAGCAGT TCTACTGCAA AAACAAGCCC ATTCGCCCCG AGGCCAGCGA CAGCCCCAGT 
CCGAATCACC TGGGCGGAGG CGTTGCCGTG GGCCTGGGCA TTGGCGGCCT GGTCGGCGGA 
CACGGCCAGC AGAAGAACAA GGAAAACCTG CAGGAGGCGG CCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush ( NM_001272900.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259829.1 Drosophila melanogaster u-shaped (ush), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15871
Clone ID Related Accession (Same CDS sequence)	NM_001272900.1
Accession Version	NM_001272900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	u-shaped, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTAGCA GCAATACAAG AGGTGATTGT TCCGATACCG CAGAAGAGAT GACCGTCGAC 
AGCAGAGATT CCAAAGATCT GAGTGCTCAG GATATAGGCG AGCAGAAGCA GCAGCAAATG 
GAGGACCAGT TGGAGGATCA GTTGAACGAT TCCAGAGACC CCCAAAACAA CAATAACAAT 
ATCGATGACG ACGCTGATGA AGATGCTGAG TTCGAGGAGC CAGAAAAGGC CAATCCCCAA 
CAGGATCAGG ATTTGGGAGA GACAGAGATG GAACAGGAAC ACGATCTTCA GCAGGAGGAT 
CTTCAGCAGG AACTGCCTGC AAACAGCCCA AGCACTCCGC CCAGGAGTCC CAGCTCTCCC 
CAGCTGATTC CCAAGCTGGA ACAGCCCGCT ACGCCGCCCT CGGAGCCAGA AGCCTCTCCG 
TGCCCCTCAC CCTCACCATG TCCCACGCCC AAGTACCCCA AAGTGCGTCT GAATGCGCTC 
CTGGCCTCAG ATCCTGCCCT TAAGCCAGAT GCCAAGGAGC TGACCCTGCC GGACTCTCGT 
CTCCTGGCTC CTCCGCCGCT CGTCAAGCCG GACACGCAAG CGCAACCGGA GGTAGCAGAA 
CCTCTGCTAA AACCAGCCAG ATTTATGTGC CTGCCCTGTG GGATTGCATT CAGTTCGCCC 
TCAACTCTGG AGGCCCATCA AGCCTACTAT TGCTCTCATA GAATTAAGGA CACGGACGAG 
GCTGGCAGTG ATAAGTCAGG GGCAGGAGGA TCGGGAGCCA CAGCTGGCGA TGCAGCAGGG 
TTGACCGGAG GCTCCACCGA GCCACCAGCC AAAATGGCCA GGACAGGAAA GCAATATGGC 
TGCACCCAGT GCTCCTACAG CGCGGATAAG AAGGTATCCC TAAATCGCCA CATGCGCATG 
CACCAGACTT CGCCAGCAGC TCCCACACTG GCCGGCTTGC CAAGTCTTCT CCAGAACGGA 
ATAGCGCCGC CGGGAGTTAC GCCCAATCCG ATGGAGGATA GCTCTAGTCA ACAAACCGAT 
CGCTACTGCA GCCACTGTGA TATCCGGTTC AACAACATCA AGACCTATCG AGCGCACAAA 
CAACACTACT GCAGTTCGCG GCGACCAGAG GGACAGCTCA CGCCCAAGCC AGACGCCTCT 
CCGGGAGCAG GTTCCGGACC GGGTTCCGCA GGCGGATCCA TAGGAGTATC AGCCCAGGCG 
GCTACGCCCG GCAAGCTGAG TCCGCAGGCA AGGAACAAGA CTCCGACTCC CGCAATGGTC 
GCCGTCGCAG CTGCAGCAGC GGCTGCCGCT GCCTCCCTCC AGGCCACGCC GCATTCGCAT 
CCACCTTTCC TGGCACTGCC CACCCACCCG ATCATAATTG TGCCCTGCTC GCTGATCCGT 
GCAGCTAGCT TTATTCCAGG CCCACTGCCC ACGCCGAACT CGGGAATCGT GAATCCTGAA 
ACCACCTGTT TTACCGTGGA CAACGGAACG ATTAAACCAC TGGCCACTGC CCTAGTTGGC 
GCCACCTTAG AACCCGAACG CCCTTCAGCT CCATCATCTG CTGCTGAGGC CACAGAAGCC 
AAGAGTAGTC CTCCGGAGCC CAAGCGAAAA GAGGCAGGGT TAACTAGAGA ATCTGCGCCT 
CTCGATCTCT CGCTGCGTCG ATCGCCAATC ACCCTGAATT CACTGAGCTT GCGTCAGCGC 
CAACTGCGGA ATGCTCTCTT AGATGTGGAA GAAGTACTCC TTGCCGGCGT AGGAACCGGC 
AAGGAGAACG TTGAGACTCC GAGAGGAGGT GGAAGTGTGA CTCCCGAGCA AATCGTTTGC 
GCTCCCTCCC TGCCGAGCAG TCCTTCGATG AGTCCCTCGC CCAAAAGACG AGCCATCAGT 
CCACGCAGTT CCGGGGCTGG TAGCGCCTCC TCCATGTCAC CACCTGGTCT CAATGTGGCT 
GTTCCCCATC TGCTCGACAT GCGCTCCATG CTACCTGCGG ACTTTGGACT CTCCGAGTCG 
CTGTTGGCCA AAACCAACCC GGAACTGGCT CTGAAACTGG CGGCGGCAGC GGCCGCAGCT 
GCTGTGGCGG GCAGTAGTGG AGCAGCAGCC TTCCCGCCCG CTTCTCTTCC TGCCCAAACT 
AGTAGTGGGA ATCCGGGTAG TGGAGGATCG GCAGGTGGCG CCCAGCAGCC TCAGATCTAT 
GTGAAAAAGG GCGTGTCCAA GTGCATGGAG TGCAATATTG TGTTTTGCAA GTATGAGAAC 
TACTTGGCAC ACAAACAGCA TTATTGCTCG GCAAGAAGCC AAGAAGGTGC GAGTGAAGTG 
GATGTCAAGT CGGCCGTTTC ACCATCCATC GCTGGAGCAG GAGGACTGGG AGCCGGAGCT 
GCAGAGGCTG CTTCGTCGGT GGAAACCACA CCGGTGGCAT ATCAACAGTT GATCTGCGCC 
GCCTGCGGAA TTAAGTACAC TTCCCTGGAC AACCTGCGAG CCCATCAGAA CTACTATTGC 
CCCAAGGGAG GAGCAGTTGC TGCCCCTGCC GCGACGCCCA CGGATCCTGG CCAACTGGGC 
ATGCCAAAGG AGAAGTGCGG AAAGTGCAAG ACATTGCATG AAATTGGTCT GCCTTGTCCA 
CCACCCGTTG CGAATCCTCT CGCAGCGCCG ACAGTCAATC CGCAACCCGC CACCAATAGT 
CTGAACAAGT GTCCTGTTTG CGGGGTAGTG AGTCCCACAG CAGCACTGGC CAAGAAACAC 
ATGGAGATGC ACGGAACAGT AAAGGCATAC CGCTGCAGCA TCTGCCAGTA CAAGGGGAAC 
ACTCTTCGCG GCATGCGCAC CCACATTCGA ACCCATTTCG ACAAGAAGAC TAGCGACGTA 
AACGAGGAGC TATACATGAC CTGCATCTTT GAAGAGGATG CTAGCGCGCT GAGTCAGGAA 
CTGGTTACTC CAACAGGGGC ATCTACCACA ACTGGACACG ATTCCATGGA TCATCCCTCG 
CAGATGTTCA ACTGTGATTA CTGCAACTAT GTCTCCACCT ACAAGGGCAA TGTGCTGCGC 
CACATGAAGC TGATGCATCC CCATGTGGCC ATCAACTCTC CCTCCATTTC CCCCGACACC 
AGAGATCAAG ATGTGACTTC CAACCCCACT ACCAACCAAC ACTCGAATTC CGATGTATCC 
AACGGTGAAG CACCCAGTTT CCACATTAAA TCGGAGCCTC TGGATCCACC ACCGACTGTG 
AATCTGGTGC ACGAGAACAA CAACTCCCCG ATCGCCACGC CACATATTAA GGCTGAGCCA 
ATCGAAGTCG GAGCGGATGC TGCGCCCGGC GGGCTGGTTC CGCCGATGAC TTCTCCGCTG 
GGAAACAGCA GCAGCGTGGC TGCCGCAGCA GCTGCCGCCG CCGAGGTGAT GAAGAAGTAC 
TGCTCAACCT GTGACATATC CTTCAATTAC GTGAAGACCT ACCTGGCTCA CAAGCAGTTC 
TACTGCAAAA ACAAGCCCAT TCGCCCCGAG GCCAGCGACA GCCCCAGTCC GAATCACCTG 
GGCGGAGGCG TTGCCGTGGG CCTGGGCATT GGCGGCCTGG TCGGCGGACA CGGCCAGCAG 
AAGAACAAGG AAAACCTGCA GGAGGCGGCC ATTTGAGAAA GCCAGCTGCG GTGGGAGGCG 
CAGCGCAAGC AGCTTGAGTG GTATTACACG TGCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259829.1
CDS	303..3941
Translation	MLSSNTRGDCSDTAEEMTVDSRDSKDLSAQDIGEQKQQQMEDQLEDQLNDSRDPQNNNNNIDDDADEDAEFEEPEKANPQQDQDLGETEMEQEHDLQQEDLQQELPANSPSTPPRSPSSPQLIPKLEQPATPPSEPEASPCPSPSPCPTPKYPKVRLNALLASDPALKPDAKELTLPDSRLLAPPPLVKPDTQAQPEVAEPLLKPARFMCLPCGIAFSSPSTLEAHQAYYCSHRIKDTDEAGSDKSGAGGSGATAGDAAGLTGGSTEPPAKMARTGKQYGCTQCSYSADKKVSLNRHMRMHQTSPAAPTLAGLPSLLQNGIAPPGVTPNPMEDSSSQQTDRYCSHCDIRFNNIKTYRAHKQHYCSSRRPEGQLTPKPDASPGAGSGPGSAGGSIGVSAQAATPGKLSPQARNKTPTPAMVAVAAAAAAAAASLQATPHSHPPFLALPTHPIIIVPCSLIRAASFIPGPLPTPNSGIVNPETTCFTVDNGTIKPLATALVGATLEPERPSAPSSAAEATEAKSSPPEPKRKEAGLTRESAPLDLSLRRSPITLNSLSLRQRQLRNALLDVEEVLLAGVGTGKENVETPRGGGSVTPEQIVCAPSLPSSPSMSPSPKRRAISPRSSGAGSASSMSPPGLNVAVPHLLDMRSMLPADFGLSESLLAKTNPELALKLAAAAAAAAVAGSSGAAAFPPASLPAQTSSGNPGSGGSAGGAQQPQIYVKKGVSKCMECNIVFCKYENYLAHKQHYCSARSQEGASEVDVKSAVSPSIAGAGGLGAGAAEAASSVETTPVAYQQLICAACGIKYTSLDNLRAHQNYYCPKGGAVAAPAATPTDPGQLGMPKEKCGKCKTLHEIGLPCPPPVANPLAAPTVNPQPATNSLNKCPVCGVVSPTAALAKKHMEMHGTVKAYRCSICQYKGNTLRGMRTHIRTHFDKKTSDVNEELYMTCIFEEDASALSQELVTPTGASTTTGHDSMDHPSQMFNCDYCNYVSTYKGNVLRHMKLMHPHVAINSPSISPDTRDQDVTSNPTTNQHSNSDVSNGEAPSFHIKSEPLDPPPTVNLVHENNNSPIATPHIKAEPIEVGADAAPGGLVPPMTSPLGNSSSVAAAAAAAAEVMKKYCSTCDISFNYVKTYLAHKQFYCKNKPIRPEASDSPSPNHLGGGVAVGLGIGGLVGGHGQQKNKENLQEAAI*ESQLRWEAQRKQLEWYYTCF

Target ORF information:

RefSeq Version	NM_001272900.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster u-shaped (ush), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272900.1

ORF Insert Sequence:

ATGCTTAGCA GCAATACAAG AGGTGATTGT TCCGATACCG CAGAAGAGAT GACCGTCGAC 
AGCAGAGATT CCAAAGATCT GAGTGCTCAG GATATAGGCG AGCAGAAGCA GCAGCAAATG 
GAGGACCAGT TGGAGGATCA GTTGAACGAT TCCAGAGACC CCCAAAACAA CAATAACAAT 
ATCGATGACG ACGCTGATGA AGATGCTGAG TTCGAGGAGC CAGAAAAGGC CAATCCCCAA 
CAGGATCAGG ATTTGGGAGA GACAGAGATG GAACAGGAAC ACGATCTTCA GCAGGAGGAT 
CTTCAGCAGG AACTGCCTGC AAACAGCCCA AGCACTCCGC CCAGGAGTCC CAGCTCTCCC 
CAGCTGATTC CCAAGCTGGA ACAGCCCGCT ACGCCGCCCT CGGAGCCAGA AGCCTCTCCG 
TGCCCCTCAC CCTCACCATG TCCCACGCCC AAGTACCCCA AAGTGCGTCT GAATGCGCTC 
CTGGCCTCAG ATCCTGCCCT TAAGCCAGAT GCCAAGGAGC TGACCCTGCC GGACTCTCGT 
CTCCTGGCTC CTCCGCCGCT CGTCAAGCCG GACACGCAAG CGCAACCGGA GGTAGCAGAA 
CCTCTGCTAA AACCAGCCAG ATTTATGTGC CTGCCCTGTG GGATTGCATT CAGTTCGCCC 
TCAACTCTGG AGGCCCATCA AGCCTACTAT TGCTCTCATA GAATTAAGGA CACGGACGAG 
GCTGGCAGTG ATAAGTCAGG GGCAGGAGGA TCGGGAGCCA CAGCTGGCGA TGCAGCAGGG 
TTGACCGGAG GCTCCACCGA GCCACCAGCC AAAATGGCCA GGACAGGAAA GCAATATGGC 
TGCACCCAGT GCTCCTACAG CGCGGATAAG AAGGTATCCC TAAATCGCCA CATGCGCATG 
CACCAGACTT CGCCAGCAGC TCCCACACTG GCCGGCTTGC CAAGTCTTCT CCAGAACGGA 
ATAGCGCCGC CGGGAGTTAC GCCCAATCCG ATGGAGGATA GCTCTAGTCA ACAAACCGAT 
CGCTACTGCA GCCACTGTGA TATCCGGTTC AACAACATCA AGACCTATCG AGCGCACAAA 
CAACACTACT GCAGTTCGCG GCGACCAGAG GGACAGCTCA CGCCCAAGCC AGACGCCTCT 
CCGGGAGCAG GTTCCGGACC GGGTTCCGCA GGCGGATCCA TAGGAGTATC AGCCCAGGCG 
GCTACGCCCG GCAAGCTGAG TCCGCAGGCA AGGAACAAGA CTCCGACTCC CGCAATGGTC 
GCCGTCGCAG CTGCAGCAGC GGCTGCCGCT GCCTCCCTCC AGGCCACGCC GCATTCGCAT 
CCACCTTTCC TGGCACTGCC CACCCACCCG ATCATAATTG TGCCCTGCTC GCTGATCCGT 
GCAGCTAGCT TTATTCCAGG CCCACTGCCC ACGCCGAACT CGGGAATCGT GAATCCTGAA 
ACCACCTGTT TTACCGTGGA CAACGGAACG ATTAAACCAC TGGCCACTGC CCTAGTTGGC 
GCCACCTTAG AACCCGAACG CCCTTCAGCT CCATCATCTG CTGCTGAGGC CACAGAAGCC 
AAGAGTAGTC CTCCGGAGCC CAAGCGAAAA GAGGCAGGGT TAACTAGAGA ATCTGCGCCT 
CTCGATCTCT CGCTGCGTCG ATCGCCAATC ACCCTGAATT CACTGAGCTT GCGTCAGCGC 
CAACTGCGGA ATGCTCTCTT AGATGTGGAA GAAGTACTCC TTGCCGGCGT AGGAACCGGC 
AAGGAGAACG TTGAGACTCC GAGAGGAGGT GGAAGTGTGA CTCCCGAGCA AATCGTTTGC 
GCTCCCTCCC TGCCGAGCAG TCCTTCGATG AGTCCCTCGC CCAAAAGACG AGCCATCAGT 
CCACGCAGTT CCGGGGCTGG TAGCGCCTCC TCCATGTCAC CACCTGGTCT CAATGTGGCT 
GTTCCCCATC TGCTCGACAT GCGCTCCATG CTACCTGCGG ACTTTGGACT CTCCGAGTCG 
CTGTTGGCCA AAACCAACCC GGAACTGGCT CTGAAACTGG CGGCGGCAGC GGCCGCAGCT 
GCTGTGGCGG GCAGTAGTGG AGCAGCAGCC TTCCCGCCCG CTTCTCTTCC TGCCCAAACT 
AGTAGTGGGA ATCCGGGTAG TGGAGGATCG GCAGGTGGCG CCCAGCAGCC TCAGATCTAT 
GTGAAAAAGG GCGTGTCCAA GTGCATGGAG TGCAATATTG TGTTTTGCAA GTATGAGAAC 
TACTTGGCAC ACAAACAGCA TTATTGCTCG GCAAGAAGCC AAGAAGGTGC GAGTGAAGTG 
GATGTCAAGT CGGCCGTTTC ACCATCCATC GCTGGAGCAG GAGGACTGGG AGCCGGAGCT 
GCAGAGGCTG CTTCGTCGGT GGAAACCACA CCGGTGGCAT ATCAACAGTT GATCTGCGCC 
GCCTGCGGAA TTAAGTACAC TTCCCTGGAC AACCTGCGAG CCCATCAGAA CTACTATTGC 
CCCAAGGGAG GAGCAGTTGC TGCCCCTGCC GCGACGCCCA CGGATCCTGG CCAACTGGGC 
ATGCCAAAGG AGAAGTGCGG AAAGTGCAAG ACATTGCATG AAATTGGTCT GCCTTGTCCA 
CCACCCGTTG CGAATCCTCT CGCAGCGCCG ACAGTCAATC CGCAACCCGC CACCAATAGT 
CTGAACAAGT GTCCTGTTTG CGGGGTAGTG AGTCCCACAG CAGCACTGGC CAAGAAACAC 
ATGGAGATGC ACGGAACAGT AAAGGCATAC CGCTGCAGCA TCTGCCAGTA CAAGGGGAAC 
ACTCTTCGCG GCATGCGCAC CCACATTCGA ACCCATTTCG ACAAGAAGAC TAGCGACGTA 
AACGAGGAGC TATACATGAC CTGCATCTTT GAAGAGGATG CTAGCGCGCT GAGTCAGGAA 
CTGGTTACTC CAACAGGGGC ATCTACCACA ACTGGACACG ATTCCATGGA TCATCCCTCG 
CAGATGTTCA ACTGTGATTA CTGCAACTAT GTCTCCACCT ACAAGGGCAA TGTGCTGCGC 
CACATGAAGC TGATGCATCC CCATGTGGCC ATCAACTCTC CCTCCATTTC CCCCGACACC 
AGAGATCAAG ATGTGACTTC CAACCCCACT ACCAACCAAC ACTCGAATTC CGATGTATCC 
AACGGTGAAG CACCCAGTTT CCACATTAAA TCGGAGCCTC TGGATCCACC ACCGACTGTG 
AATCTGGTGC ACGAGAACAA CAACTCCCCG ATCGCCACGC CACATATTAA GGCTGAGCCA 
ATCGAAGTCG GAGCGGATGC TGCGCCCGGC GGGCTGGTTC CGCCGATGAC TTCTCCGCTG 
GGAAACAGCA GCAGCGTGGC TGCCGCAGCA GCTGCCGCCG CCGAGGTGAT GAAGAAGTAC 
TGCTCAACCT GTGACATATC CTTCAATTAC GTGAAGACCT ACCTGGCTCA CAAGCAGTTC 
TACTGCAAAA ACAAGCCCAT TCGCCCCGAG GCCAGCGACA GCCCCAGTCC GAATCACCTG 
GGCGGAGGCG TTGCCGTGGG CCTGGGCATT GGCGGCCTGG TCGGCGGACA CGGCCAGCAG 
AAGAACAAGG AAAACCTGCA GGAGGCGGCC ATTTGAGAAA GCCAGCTGCG GTGGGAGGCG 
CAGCGCAAGC AGCTTGAGTG GTATTACACG TGCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ush cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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