ex cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ex
Entrez Gene ID	33218
Full Name	expanded
Synonyms	1270,CG4114,Dmel\CG4114,Ex,Expanded,brl,l(2)01270,l(2)ey
General protein information	Preferred Names expanded Names CG4114 gene product from transcript CG4114-RA CG4114-PA CG4114-PB braille ex-PA ex-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21C2-21C2\|2-0.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057492.4	NP_476840.2	expanded, isoform A
NM_001272894.1	NP_001259823.1	expanded, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009126	XP_319884.4
Drosophila melanogaster (fruit fly)	ex	NP_001259823.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Merlin and Kibra activate Hippo signaling in parallel to Expanded at a spatially distinct cellular domain, the medial apical cortex.
Title: Kibra and Merlin Activate the Hippo Pathway Spatially Distinct from and Independent of Expanded.

results show that Zyxin is a functional antagonist of Expanded in growth control
Title: Zyxin antagonizes the FERM protein expanded to couple F-actin and Yorkie-dependent organ growth.

The Ex-regulatory domain of Crb maps to the juxtamembrane FERM-binding motif (JM), a cytoskeletal interaction domain distinct from the PDZ-binding motif (PBM) through which Crb binds polarity factors.
Title: Crumbs regulates Salvador/Warts/Hippo signaling in Drosophila via the FERM-domain protein Expanded.

Kibra functions together with Mer and Ex in a protein complex localized to the apical domain of epithelial cells, and this protein complex regulates the Hippo kinase cascade via direct binding to Hpo and Sav.
Title: Kibra functions as a tumor suppressor protein that regulates Hippo signaling in conjunction with Merlin and Expanded.

Mer and Ex signal through the Hippo pathway in Drosophila.
Title: The Hippo tumor-suppressor pathway regulates apical-domain size in parallel to tissue growth.

The following ex gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ex cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13349	NM_001272894.1 Latest version!	Drosophila melanogaster expanded (ex), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa13349	NM_057492.4 Latest version!	Drosophila melanogaster expanded (ex), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ex ( NM_057492.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476840.2 Drosophila melanogaster expanded (ex), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13349
Clone ID Related Accession (Same CDS sequence)	NM_057492.4 , NM_001272894.1
Accession Version	NM_057492.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	expanded, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057492.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAGCAT TTTGCACCGT CAGCGCGCCT TTGGAAGTAT GCGCGTCCTC AGCCGAGCAA 
CTATCACCAG GCTCGCGATT CCTGGCCCTG AGACTTCTGG GGCAGCAGCA GCCGAAAACC 
TTGTACTTCC TAGTCGATGC CAAGAGTCGA GTTCGAGAGG TTTACACGCA GACATGTCTG 
CACTTTGCCA CGCAAGGGAT GCTGGACACC GAACTCTTCG GTCTGGCTGT GCTCATAGAT 
GGCGAGTACA TGTTCGCAGA TCCCGAGAGC AAGCTCTCAA AATATGGCCC AAAGAGCTGG 
CGATCATCGC ACACCCACGG GCTGGATGCC AACGGACGAC CGCTCCTGGA GCTACACTTT 
CGCGTGCAGT TCTACATCGA GAGCCCGTTC ATGCTGAAAG ATGAGACGTC CCGCCATAAT 
TACTACCTGC AGCTGCGCCA CAACATCTTG CAGCGCGATT TGCCGAGAGA ACAGGCGGAG 
CAGGCACTGG TCTTCCTTGC TGGACTTGCT CTTCAGGCGG ACCTGGGTGA TGCTCCTCCG 
GGCACGAGTA ATTCCAAGGA TGATTCAGGA GAGGAGACAT CGGCATCTCC TTCTAACGGA 
GGAAGAGGTC TAAGTGCCAC CACTACATTA CCCAAGATCA GCAAACGTGC TAATGAGAGG 
ATGCTTAGAC TCTCGACATA TGTGGCGTCC ACATCAAAGA GAGAAACAAT ACCATTACCA 
CCCAGCCTGC CACCGAATGG AGCAGATTAC TACCGGATCG AGGATTACCT ACCCAGTGGA 
CTGCACACTC CCTGGGCGAG AAGTGCAATG AGAGCCTGTC ATCGGGAGCA TTTGGGCATG 
GCCACGGCAG AGGCGGAGCT GCTATATATT CAACAGGCAT GCAGTCTTCA TGAAACCATC 
AATGCCCATA CCTACAGGAT GCGATTGGCC AAAAGCGAGC AGGGATCTGG TAGTGCCTGG 
TTTGTGGTCT ATGCCAAGGG AATCAAGATT CTGGGAGGAG AGTCTACCAA CAGCTCTTCC 
AATCCCGAAA CCACAACCTT TCTGTGGCCG AATATCACGA AGCTCTCCTT CGAGCGGAAA 
AAGTTTGAAA TACGATCTGG AGAGAGCCGA ATCACCCTGT ACGCCGCATC CGATGAGAAA 
AATAAACTCC TATTGACCCT CTGCAAGGAT ACACATCAGT GGAGCATGAA ACTGGCTGCC 
AGGCTAAAGG AGGTATCCAA ACGCGAGGAG GAGGAGGCTG CCGAATCACA AAGGCTACAT 
GCCAGCTACG CTTGTTCCAG AAGTCTGCTC TTGCCCTACA AAAGTAAAAA CGAGCAGCGA 
ATATCGGTTA TCTCGAGTAC CAGCTCCAAC ACAACCTCTG GGATAGTAAG TGATCGAGTG 
CACTCCGAAG ACGAGCTGGA GATCATGATC AATACACCAC CAGCACCCTT AGCTGCTCCG 
TCAACGGAAA GTTTGGCCTT GGCTCATCTT TTGGACAGAC CCAGTGTGAG CAGACAAACT 
TCGAGCGTGG GTCAGATGTC TCTGAAGGAT TTGGAAGAAC AACTGGCTGC CTTGAGTGTG 
AGGCCACAAG ACGCGAGTTC TAACGGGGCC ACGATCGTCA CGAACTCCTC TGTTCAGAGA 
AATTCCATGG GAACAACTGC CAATGATTCA TCTACCGCCA CTGATTCACC CAGTTCGCAG 
CATAACATCG GCTCTCAGTG CTCATCCACT TGTAGCACTG TGGTGGTAAC ATCTCCTGTA 
AATGGAGCAG GCGCTTCCTC TTCAGGTGCT CCCATACCTG TGCACTCCAC TTCATCTAGT 
TTGGAGCTTG GCTTTAGTCA CACCGCTCAA AACTCGGCCT TGAGCGAAAC GAGTCCCGAC 
GATTTTCTCT CCACATCGGC AAGAGAAGAA ACGGAGAGTG TATCGGGCGC ATCTGGGGTT 
TATACTTTGG CGCACGGAGC TCCACCCACG GAAACCTCAG GCGTTTACAC CATGCACAGC 
AGTGAGCTAA CTGGACAATC ATCGGAGATC GCCGAGTCGG AAAAGAGTTC GCACTACGGG 
ATGTTCCAGC CCCAAAAACT AGAAGAAACC CACGTGCAAC ATTCTGATTC CGTAGATGGA 
AAAAAGAAAG AAGACTTCAG ACCGCGATCG GATTCTAACG TCAGCACAGG GAGTTCCTTT 
AGAGGCGATG GCAGCGATCC AACAGACAAC AAACATTCCT TGCTCTCCGC AGAAGAGTTA 
ACCAATTTGA TTGTGGGCAG GGGGACCTAC CCCAGTCGGA AAACAGTATC TAGTAGCCTT 
CACTCGGACT GTGATTACGT GACGTTACCA CTAGGAGATC AAGGAGAAGA GGAGGTAGAC 
CAACCACCAG CACCACCACC ACCCTACAGT GCTAGGCATG AGAAAACTGG TCTCTGTGGC 
CCACCTATCG CCAAACCTAT CCCTAAACCT ATAGCTGTGG TAGCTCCCAA ACCAGATTCA 
CCGCCATGTA GTCCACCGGT ACCGCCAGCA CCAATTCCAG CCCCACCGCC GGCTATTCGA 
CGAAGAGATC CACCGCCATA TTCCATCTCT AGCAAGCCTA GACCCACATC CCTGATATCT 
GTCAGTTCGT CGGCACATCC TGCGCCCAGT GCTGCCGGCT CGATGAGTTC TTTAAAGTCC 
GAGGAGGTTA CAGCCAGATT TATCACCACC AGGCCACAGA TTAGTATTCT GAAAGCCCAC 
ACCAGCCTGA TTCCCGATGG GGCCAAGCCA AGTTACGCGG CACCGCACCA TTGTTCATCA 
GTTGCCTCCT CCAATGGATC GGTTTGCAGC CATCAGTTGA GTCAGCAATC GCTGCATAAC 
TCCAACTATG CCGGCGGATC CCAGGCTTCA CTGCACCATC ATCACGTGCC GTCACACCAC 
CGACATTCTG GATCGGCAGC CATTGGAATA GTTCCTTACG GTCTGCACAA GAGCACGGCA 
TCACTGCATC ATCAGCAGTC ATGTGTACTA CTACCGGTTA TTAAACCTCG GCAGTTCCTG 
GCACCACCAC CGCCCAGTTT GCCGCGGCAG CCTCCACCAC CGCCCCCACC GAATCACCCC 
CATTTGGCGA GTCACCTTTA CGAGAGGGAA ATGGCTAGAA AACAATTGGA GCTCTACCAG 
CAGCAGCTTT ACAGCGATGT GGATTACGTA ATTTACCCCA TCCAGGATCC AGCTGTAAGC 
CAGCAAGAGT ATCTGGATGC CAAACAGGGA TCCTTACTGG CGGCAATGGC CCAAGCAGCA 
CCACCCCCAC CGCACCACCC ATATCTGGCC ATGCAGGTGT CGCCGGCGAT TTACAGAAGC 
ACCCCCTACC TACCACTGAC TCTGTCGACC CACTCACGAT ATGCATCCAC CCAAAATCTA 
TCGGATACAT ATGTGCAGCT TCCAGGACCC GGATACTCAC CCCTGTACTC TCCTTCGATG 
GCCAGCCTAT GCTCCTCGTA TGAGCCTCCA CCGCCTCCTC CCCTTCACCC GGCTGCTCTA 
GCAGCAGCTG CTGCGGCGGG GGCTGGCTCC TCGTCTTCCT CGATGTTTGC GCGATCGCGA 
TCAGATGACA ATATTCTGAA CTCACTAGAT TTGCTGCCCA AGGGAAAACG ACTGCCACCG 
CCGCCACCAC CACCGTATGT AAACAGACGG CTAAAGAAAC CACCCATGCC GGCGCCCAGT 
GAAAAGCCAC CTCCAATACC CTCTAAGCCA ATTCCTAGCA GGATGAGCCC GATTCCACCA 
CGAAAACCCC CAACGTTGAA TCCGCATCAT GCGAACTCAC CGCTAACAAA GACCTCCAGC 
GGCGCCCAGT GGGCAGGTGA GCGTCCGAGA CCTGACTTGG GTCTTGGTCT GGGATTGAAC 
CGGGGAAACA ACAGCATTTT GGCCCAGCTG CAGGCTTCAA TGGTAGCCCA GTCTCATGCC 
CAGGCACAGG CCCAAGCGCT AGACATTGCG CTTCTTCGGG AGAAGAGCAA GCATCTAGAT 
CTGCCGCTGA TCTCGGCGCT CTGCAACGAT CGCTCCTTGC TGAAACAGAC TAAGGTTGTT 
ATAAATCCGA AGACTGGCCA GGAGATGCCC ACTTCCAGTG CGCAACCTTC AGGTGCCACC 
ACAAATGGAG TTGCCAACTC CTCTGCAGGA GCAGGAACCC TAAGCAAGGC CAGAAAAGGA 
TCTACCGTAA GCCACCGTCA TCCGCAGGAC AAACTGCCGC CTTTACCTGT CCAACAATTG 
GCGGAGGCCA ACAACTACGT GATCGATCCC GCTGTCATGA TGAAGCAACA GCAGCAGCAG 
CAGCAGCACA ACAAGACCAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476840.2
CDS	937..5220
Translation	MRAFCTVSAPLEVCASSAEQLSPGSRFLALRLLGQQQPKTLYFLVDAKSRVREVYTQTCLHFATQGMLDTELFGLAVLIDGEYMFADPESKLSKYGPKSWRSSHTHGLDANGRPLLELHFRVQFYIESPFMLKDETSRHNYYLQLRHNILQRDLPREQAEQALVFLAGLALQADLGDAPPGTSNSKDDSGEETSASPSNGGRGLSATTTLPKISKRANERMLRLSTYVASTSKRETIPLPPSLPPNGADYYRIEDYLPSGLHTPWARSAMRACHREHLGMATAEAELLYIQQACSLHETINAHTYRMRLAKSEQGSGSAWFVVYAKGIKILGGESTNSSSNPETTTFLWPNITKLSFERKKFEIRSGESRITLYAASDEKNKLLLTLCKDTHQWSMKLAARLKEVSKREEEEAAESQRLHASYACSRSLLLPYKSKNEQRISVISSTSSNTTSGIVSDRVHSEDELEIMINTPPAPLAAPSTESLALAHLLDRPSVSRQTSSVGQMSLKDLEEQLAALSVRPQDASSNGATIVTNSSVQRNSMGTTANDSSTATDSPSSQHNIGSQCSSTCSTVVVTSPVNGAGASSSGAPIPVHSTSSSLELGFSHTAQNSALSETSPDDFLSTSAREETESVSGASGVYTLAHGAPPTETSGVYTMHSSELTGQSSEIAESEKSSHYGMFQPQKLEETHVQHSDSVDGKKKEDFRPRSDSNVSTGSSFRGDGSDPTDNKHSLLSAEELTNLIVGRGTYPSRKTVSSSLHSDCDYVTLPLGDQGEEEVDQPPAPPPPYSARHEKTGLCGPPIAKPIPKPIAVVAPKPDSPPCSPPVPPAPIPAPPPAIRRRDPPPYSISSKPRPTSLISVSSSAHPAPSAAGSMSSLKSEEVTARFITTRPQISILKAHTSLIPDGAKPSYAAPHHCSSVASSNGSVCSHQLSQQSLHNSNYAGGSQASLHHHHVPSHHRHSGSAAIGIVPYGLHKSTASLHHQQSCVLLPVIKPRQFLAPPPPSLPRQPPPPPPPNHPHLASHLYEREMARKQLELYQQQLYSDVDYVIYPIQDPAVSQQEYLDAKQGSLLAAMAQAAPPPPHHPYLAMQVSPAIYRSTPYLPLTLSTHSRYASTQNLSDTYVQLPGPGYSPLYSPSMASLCSSYEPPPPPPLHPAALAAAAAAGAGSSSSSMFARSRSDDNILNSLDLLPKGKRLPPPPPPPYVNRRLKKPPMPAPSEKPPPIPSKPIPSRMSPIPPRKPPTLNPHHANSPLTKTSSGAQWAGERPRPDLGLGLGLNRGNNSILAQLQASMVAQSHAQAQAQALDIALLREKSKHLDLPLISALCNDRSLLKQTKVVINPKTGQEMPTSSAQPSGATTNGVANSSAGAGTLSKARKGSTVSHRHPQDKLPPLPVQQLAEANNYVIDPAVMMKQQQQQQQHNKTS

Target ORF information:

RefSeq Version	NM_057492.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster expanded (ex), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057492.4

ORF Insert Sequence:

ATGCGAGCAT TTTGCACCGT CAGCGCGCCT TTGGAAGTAT GCGCGTCCTC AGCCGAGCAA 
CTATCACCAG GCTCGCGATT CCTGGCCCTG AGACTTCTGG GGCAGCAGCA GCCGAAAACC 
TTGTACTTCC TAGTCGATGC CAAGAGTCGA GTTCGAGAGG TTTACACGCA GACATGTCTG 
CACTTTGCCA CGCAAGGGAT GCTGGACACC GAACTCTTCG GTCTGGCTGT GCTCATAGAT 
GGCGAGTACA TGTTCGCAGA TCCCGAGAGC AAGCTCTCAA AATATGGCCC AAAGAGCTGG 
CGATCATCGC ACACCCACGG GCTGGATGCC AACGGACGAC CGCTCCTGGA GCTACACTTT 
CGCGTGCAGT TCTACATCGA GAGCCCGTTC ATGCTGAAAG ATGAGACGTC CCGCCATAAT 
TACTACCTGC AGCTGCGCCA CAACATCTTG CAGCGCGATT TGCCGAGAGA ACAGGCGGAG 
CAGGCACTGG TCTTCCTTGC TGGACTTGCT CTTCAGGCGG ACCTGGGTGA TGCTCCTCCG 
GGCACGAGTA ATTCCAAGGA TGATTCAGGA GAGGAGACAT CGGCATCTCC TTCTAACGGA 
GGAAGAGGTC TAAGTGCCAC CACTACATTA CCCAAGATCA GCAAACGTGC TAATGAGAGG 
ATGCTTAGAC TCTCGACATA TGTGGCGTCC ACATCAAAGA GAGAAACAAT ACCATTACCA 
CCCAGCCTGC CACCGAATGG AGCAGATTAC TACCGGATCG AGGATTACCT ACCCAGTGGA 
CTGCACACTC CCTGGGCGAG AAGTGCAATG AGAGCCTGTC ATCGGGAGCA TTTGGGCATG 
GCCACGGCAG AGGCGGAGCT GCTATATATT CAACAGGCAT GCAGTCTTCA TGAAACCATC 
AATGCCCATA CCTACAGGAT GCGATTGGCC AAAAGCGAGC AGGGATCTGG TAGTGCCTGG 
TTTGTGGTCT ATGCCAAGGG AATCAAGATT CTGGGAGGAG AGTCTACCAA CAGCTCTTCC 
AATCCCGAAA CCACAACCTT TCTGTGGCCG AATATCACGA AGCTCTCCTT CGAGCGGAAA 
AAGTTTGAAA TACGATCTGG AGAGAGCCGA ATCACCCTGT ACGCCGCATC CGATGAGAAA 
AATAAACTCC TATTGACCCT CTGCAAGGAT ACACATCAGT GGAGCATGAA ACTGGCTGCC 
AGGCTAAAGG AGGTATCCAA ACGCGAGGAG GAGGAGGCTG CCGAATCACA AAGGCTACAT 
GCCAGCTACG CTTGTTCCAG AAGTCTGCTC TTGCCCTACA AAAGTAAAAA CGAGCAGCGA 
ATATCGGTTA TCTCGAGTAC CAGCTCCAAC ACAACCTCTG GGATAGTAAG TGATCGAGTG 
CACTCCGAAG ACGAGCTGGA GATCATGATC AATACACCAC CAGCACCCTT AGCTGCTCCG 
TCAACGGAAA GTTTGGCCTT GGCTCATCTT TTGGACAGAC CCAGTGTGAG CAGACAAACT 
TCGAGCGTGG GTCAGATGTC TCTGAAGGAT TTGGAAGAAC AACTGGCTGC CTTGAGTGTG 
AGGCCACAAG ACGCGAGTTC TAACGGGGCC ACGATCGTCA CGAACTCCTC TGTTCAGAGA 
AATTCCATGG GAACAACTGC CAATGATTCA TCTACCGCCA CTGATTCACC CAGTTCGCAG 
CATAACATCG GCTCTCAGTG CTCATCCACT TGTAGCACTG TGGTGGTAAC ATCTCCTGTA 
AATGGAGCAG GCGCTTCCTC TTCAGGTGCT CCCATACCTG TGCACTCCAC TTCATCTAGT 
TTGGAGCTTG GCTTTAGTCA CACCGCTCAA AACTCGGCCT TGAGCGAAAC GAGTCCCGAC 
GATTTTCTCT CCACATCGGC AAGAGAAGAA ACGGAGAGTG TATCGGGCGC ATCTGGGGTT 
TATACTTTGG CGCACGGAGC TCCACCCACG GAAACCTCAG GCGTTTACAC CATGCACAGC 
AGTGAGCTAA CTGGACAATC ATCGGAGATC GCCGAGTCGG AAAAGAGTTC GCACTACGGG 
ATGTTCCAGC CCCAAAAACT AGAAGAAACC CACGTGCAAC ATTCTGATTC CGTAGATGGA 
AAAAAGAAAG AAGACTTCAG ACCGCGATCG GATTCTAACG TCAGCACAGG GAGTTCCTTT 
AGAGGCGATG GCAGCGATCC AACAGACAAC AAACATTCCT TGCTCTCCGC AGAAGAGTTA 
ACCAATTTGA TTGTGGGCAG GGGGACCTAC CCCAGTCGGA AAACAGTATC TAGTAGCCTT 
CACTCGGACT GTGATTACGT GACGTTACCA CTAGGAGATC AAGGAGAAGA GGAGGTAGAC 
CAACCACCAG CACCACCACC ACCCTACAGT GCTAGGCATG AGAAAACTGG TCTCTGTGGC 
CCACCTATCG CCAAACCTAT CCCTAAACCT ATAGCTGTGG TAGCTCCCAA ACCAGATTCA 
CCGCCATGTA GTCCACCGGT ACCGCCAGCA CCAATTCCAG CCCCACCGCC GGCTATTCGA 
CGAAGAGATC CACCGCCATA TTCCATCTCT AGCAAGCCTA GACCCACATC CCTGATATCT 
GTCAGTTCGT CGGCACATCC TGCGCCCAGT GCTGCCGGCT CGATGAGTTC TTTAAAGTCC 
GAGGAGGTTA CAGCCAGATT TATCACCACC AGGCCACAGA TTAGTATTCT GAAAGCCCAC 
ACCAGCCTGA TTCCCGATGG GGCCAAGCCA AGTTACGCGG CACCGCACCA TTGTTCATCA 
GTTGCCTCCT CCAATGGATC GGTTTGCAGC CATCAGTTGA GTCAGCAATC GCTGCATAAC 
TCCAACTATG CCGGCGGATC CCAGGCTTCA CTGCACCATC ATCACGTGCC GTCACACCAC 
CGACATTCTG GATCGGCAGC CATTGGAATA GTTCCTTACG GTCTGCACAA GAGCACGGCA 
TCACTGCATC ATCAGCAGTC ATGTGTACTA CTACCGGTTA TTAAACCTCG GCAGTTCCTG 
GCACCACCAC CGCCCAGTTT GCCGCGGCAG CCTCCACCAC CGCCCCCACC GAATCACCCC 
CATTTGGCGA GTCACCTTTA CGAGAGGGAA ATGGCTAGAA AACAATTGGA GCTCTACCAG 
CAGCAGCTTT ACAGCGATGT GGATTACGTA ATTTACCCCA TCCAGGATCC AGCTGTAAGC 
CAGCAAGAGT ATCTGGATGC CAAACAGGGA TCCTTACTGG CGGCAATGGC CCAAGCAGCA 
CCACCCCCAC CGCACCACCC ATATCTGGCC ATGCAGGTGT CGCCGGCGAT TTACAGAAGC 
ACCCCCTACC TACCACTGAC TCTGTCGACC CACTCACGAT ATGCATCCAC CCAAAATCTA 
TCGGATACAT ATGTGCAGCT TCCAGGACCC GGATACTCAC CCCTGTACTC TCCTTCGATG 
GCCAGCCTAT GCTCCTCGTA TGAGCCTCCA CCGCCTCCTC CCCTTCACCC GGCTGCTCTA 
GCAGCAGCTG CTGCGGCGGG GGCTGGCTCC TCGTCTTCCT CGATGTTTGC GCGATCGCGA 
TCAGATGACA ATATTCTGAA CTCACTAGAT TTGCTGCCCA AGGGAAAACG ACTGCCACCG 
CCGCCACCAC CACCGTATGT AAACAGACGG CTAAAGAAAC CACCCATGCC GGCGCCCAGT 
GAAAAGCCAC CTCCAATACC CTCTAAGCCA ATTCCTAGCA GGATGAGCCC GATTCCACCA 
CGAAAACCCC CAACGTTGAA TCCGCATCAT GCGAACTCAC CGCTAACAAA GACCTCCAGC 
GGCGCCCAGT GGGCAGGTGA GCGTCCGAGA CCTGACTTGG GTCTTGGTCT GGGATTGAAC 
CGGGGAAACA ACAGCATTTT GGCCCAGCTG CAGGCTTCAA TGGTAGCCCA GTCTCATGCC 
CAGGCACAGG CCCAAGCGCT AGACATTGCG CTTCTTCGGG AGAAGAGCAA GCATCTAGAT 
CTGCCGCTGA TCTCGGCGCT CTGCAACGAT CGCTCCTTGC TGAAACAGAC TAAGGTTGTT 
ATAAATCCGA AGACTGGCCA GGAGATGCCC ACTTCCAGTG CGCAACCTTC AGGTGCCACC 
ACAAATGGAG TTGCCAACTC CTCTGCAGGA GCAGGAACCC TAAGCAAGGC CAGAAAAGGA 
TCTACCGTAA GCCACCGTCA TCCGCAGGAC AAACTGCCGC CTTTACCTGT CCAACAATTG 
GCGGAGGCCA ACAACTACGT GATCGATCCC GCTGTCATGA TGAAGCAACA GCAGCAGCAG 
CAGCAGCACA ACAAGACCAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ex ( NM_001272894.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259823.1 Drosophila melanogaster expanded (ex), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13349
Clone ID Related Accession (Same CDS sequence)	NM_057492.4 , NM_001272894.1
Accession Version	NM_001272894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	expanded, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAGCAT TTTGCACCGT CAGCGCGCCT TTGGAAGTAT GCGCGTCCTC AGCCGAGCAA 
CTATCACCAG GCTCGCGATT CCTGGCCCTG AGACTTCTGG GGCAGCAGCA GCCGAAAACC 
TTGTACTTCC TAGTCGATGC CAAGAGTCGA GTTCGAGAGG TTTACACGCA GACATGTCTG 
CACTTTGCCA CGCAAGGGAT GCTGGACACC GAACTCTTCG GTCTGGCTGT GCTCATAGAT 
GGCGAGTACA TGTTCGCAGA TCCCGAGAGC AAGCTCTCAA AATATGGCCC AAAGAGCTGG 
CGATCATCGC ACACCCACGG GCTGGATGCC AACGGACGAC CGCTCCTGGA GCTACACTTT 
CGCGTGCAGT TCTACATCGA GAGCCCGTTC ATGCTGAAAG ATGAGACGTC CCGCCATAAT 
TACTACCTGC AGCTGCGCCA CAACATCTTG CAGCGCGATT TGCCGAGAGA ACAGGCGGAG 
CAGGCACTGG TCTTCCTTGC TGGACTTGCT CTTCAGGCGG ACCTGGGTGA TGCTCCTCCG 
GGCACGAGTA ATTCCAAGGA TGATTCAGGA GAGGAGACAT CGGCATCTCC TTCTAACGGA 
GGAAGAGGTC TAAGTGCCAC CACTACATTA CCCAAGATCA GCAAACGTGC TAATGAGAGG 
ATGCTTAGAC TCTCGACATA TGTGGCGTCC ACATCAAAGA GAGAAACAAT ACCATTACCA 
CCCAGCCTGC CACCGAATGG AGCAGATTAC TACCGGATCG AGGATTACCT ACCCAGTGGA 
CTGCACACTC CCTGGGCGAG AAGTGCAATG AGAGCCTGTC ATCGGGAGCA TTTGGGCATG 
GCCACGGCAG AGGCGGAGCT GCTATATATT CAACAGGCAT GCAGTCTTCA TGAAACCATC 
AATGCCCATA CCTACAGGAT GCGATTGGCC AAAAGCGAGC AGGGATCTGG TAGTGCCTGG 
TTTGTGGTCT ATGCCAAGGG AATCAAGATT CTGGGAGGAG AGTCTACCAA CAGCTCTTCC 
AATCCCGAAA CCACAACCTT TCTGTGGCCG AATATCACGA AGCTCTCCTT CGAGCGGAAA 
AAGTTTGAAA TACGATCTGG AGAGAGCCGA ATCACCCTGT ACGCCGCATC CGATGAGAAA 
AATAAACTCC TATTGACCCT CTGCAAGGAT ACACATCAGT GGAGCATGAA ACTGGCTGCC 
AGGCTAAAGG AGGTATCCAA ACGCGAGGAG GAGGAGGCTG CCGAATCACA AAGGCTACAT 
GCCAGCTACG CTTGTTCCAG AAGTCTGCTC TTGCCCTACA AAAGTAAAAA CGAGCAGCGA 
ATATCGGTTA TCTCGAGTAC CAGCTCCAAC ACAACCTCTG GGATAGTAAG TGATCGAGTG 
CACTCCGAAG ACGAGCTGGA GATCATGATC AATACACCAC CAGCACCCTT AGCTGCTCCG 
TCAACGGAAA GTTTGGCCTT GGCTCATCTT TTGGACAGAC CCAGTGTGAG CAGACAAACT 
TCGAGCGTGG GTCAGATGTC TCTGAAGGAT TTGGAAGAAC AACTGGCTGC CTTGAGTGTG 
AGGCCACAAG ACGCGAGTTC TAACGGGGCC ACGATCGTCA CGAACTCCTC TGTTCAGAGA 
AATTCCATGG GAACAACTGC CAATGATTCA TCTACCGCCA CTGATTCACC CAGTTCGCAG 
CATAACATCG GCTCTCAGTG CTCATCCACT TGTAGCACTG TGGTGGTAAC ATCTCCTGTA 
AATGGAGCAG GCGCTTCCTC TTCAGGTGCT CCCATACCTG TGCACTCCAC TTCATCTAGT 
TTGGAGCTTG GCTTTAGTCA CACCGCTCAA AACTCGGCCT TGAGCGAAAC GAGTCCCGAC 
GATTTTCTCT CCACATCGGC AAGAGAAGAA ACGGAGAGTG TATCGGGCGC ATCTGGGGTT 
TATACTTTGG CGCACGGAGC TCCACCCACG GAAACCTCAG GCGTTTACAC CATGCACAGC 
AGTGAGCTAA CTGGACAATC ATCGGAGATC GCCGAGTCGG AAAAGAGTTC GCACTACGGG 
ATGTTCCAGC CCCAAAAACT AGAAGAAACC CACGTGCAAC ATTCTGATTC CGTAGATGGA 
AAAAAGAAAG AAGACTTCAG ACCGCGATCG GATTCTAACG TCAGCACAGG GAGTTCCTTT 
AGAGGCGATG GCAGCGATCC AACAGACAAC AAACATTCCT TGCTCTCCGC AGAAGAGTTA 
ACCAATTTGA TTGTGGGCAG GGGGACCTAC CCCAGTCGGA AAACAGTATC TAGTAGCCTT 
CACTCGGACT GTGATTACGT GACGTTACCA CTAGGAGATC AAGGAGAAGA GGAGGTAGAC 
CAACCACCAG CACCACCACC ACCCTACAGT GCTAGGCATG AGAAAACTGG TCTCTGTGGC 
CCACCTATCG CCAAACCTAT CCCTAAACCT ATAGCTGTGG TAGCTCCCAA ACCAGATTCA 
CCGCCATGTA GTCCACCGGT ACCGCCAGCA CCAATTCCAG CCCCACCGCC GGCTATTCGA 
CGAAGAGATC CACCGCCATA TTCCATCTCT AGCAAGCCTA GACCCACATC CCTGATATCT 
GTCAGTTCGT CGGCACATCC TGCGCCCAGT GCTGCCGGCT CGATGAGTTC TTTAAAGTCC 
GAGGAGGTTA CAGCCAGATT TATCACCACC AGGCCACAGA TTAGTATTCT GAAAGCCCAC 
ACCAGCCTGA TTCCCGATGG GGCCAAGCCA AGTTACGCGG CACCGCACCA TTGTTCATCA 
GTTGCCTCCT CCAATGGATC GGTTTGCAGC CATCAGTTGA GTCAGCAATC GCTGCATAAC 
TCCAACTATG CCGGCGGATC CCAGGCTTCA CTGCACCATC ATCACGTGCC GTCACACCAC 
CGACATTCTG GATCGGCAGC CATTGGAATA GTTCCTTACG GTCTGCACAA GAGCACGGCA 
TCACTGCATC ATCAGCAGTC ATGTGTACTA CTACCGGTTA TTAAACCTCG GCAGTTCCTG 
GCACCACCAC CGCCCAGTTT GCCGCGGCAG CCTCCACCAC CGCCCCCACC GAATCACCCC 
CATTTGGCGA GTCACCTTTA CGAGAGGGAA ATGGCTAGAA AACAATTGGA GCTCTACCAG 
CAGCAGCTTT ACAGCGATGT GGATTACGTA ATTTACCCCA TCCAGGATCC AGCTGTAAGC 
CAGCAAGAGT ATCTGGATGC CAAACAGGGA TCCTTACTGG CGGCAATGGC CCAAGCAGCA 
CCACCCCCAC CGCACCACCC ATATCTGGCC ATGCAGGTGT CGCCGGCGAT TTACAGAAGC 
ACCCCCTACC TACCACTGAC TCTGTCGACC CACTCACGAT ATGCATCCAC CCAAAATCTA 
TCGGATACAT ATGTGCAGCT TCCAGGACCC GGATACTCAC CCCTGTACTC TCCTTCGATG 
GCCAGCCTAT GCTCCTCGTA TGAGCCTCCA CCGCCTCCTC CCCTTCACCC GGCTGCTCTA 
GCAGCAGCTG CTGCGGCGGG GGCTGGCTCC TCGTCTTCCT CGATGTTTGC GCGATCGCGA 
TCAGATGACA ATATTCTGAA CTCACTAGAT TTGCTGCCCA AGGGAAAACG ACTGCCACCG 
CCGCCACCAC CACCGTATGT AAACAGACGG CTAAAGAAAC CACCCATGCC GGCGCCCAGT 
GAAAAGCCAC CTCCAATACC CTCTAAGCCA ATTCCTAGCA GGATGAGCCC GATTCCACCA 
CGAAAACCCC CAACGTTGAA TCCGCATCAT GCGAACTCAC CGCTAACAAA GACCTCCAGC 
GGCGCCCAGT GGGCAGGTGA GCGTCCGAGA CCTGACTTGG GTCTTGGTCT GGGATTGAAC 
CGGGGAAACA ACAGCATTTT GGCCCAGCTG CAGGCTTCAA TGGTAGCCCA GTCTCATGCC 
CAGGCACAGG CCCAAGCGCT AGACATTGCG CTTCTTCGGG AGAAGAGCAA GCATCTAGAT 
CTGCCGCTGA TCTCGGCGCT CTGCAACGAT CGCTCCTTGC TGAAACAGAC TAAGGTTGTT 
ATAAATCCGA AGACTGGCCA GGAGATGCCC ACTTCCAGTG CGCAACCTTC AGGTGCCACC 
ACAAATGGAG TTGCCAACTC CTCTGCAGGA GCAGGAACCC TAAGCAAGGC CAGAAAAGGA 
TCTACCGTAA GCCACCGTCA TCCGCAGGAC AAACTGCCGC CTTTACCTGT CCAACAATTG 
GCGGAGGCCA ACAACTACGT GATCGATCCC GCTGTCATGA TGAAGCAACA GCAGCAGCAG 
CAGCAGCACA ACAAGACCAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259823.1
CDS	719..5002
Translation	MRAFCTVSAPLEVCASSAEQLSPGSRFLALRLLGQQQPKTLYFLVDAKSRVREVYTQTCLHFATQGMLDTELFGLAVLIDGEYMFADPESKLSKYGPKSWRSSHTHGLDANGRPLLELHFRVQFYIESPFMLKDETSRHNYYLQLRHNILQRDLPREQAEQALVFLAGLALQADLGDAPPGTSNSKDDSGEETSASPSNGGRGLSATTTLPKISKRANERMLRLSTYVASTSKRETIPLPPSLPPNGADYYRIEDYLPSGLHTPWARSAMRACHREHLGMATAEAELLYIQQACSLHETINAHTYRMRLAKSEQGSGSAWFVVYAKGIKILGGESTNSSSNPETTTFLWPNITKLSFERKKFEIRSGESRITLYAASDEKNKLLLTLCKDTHQWSMKLAARLKEVSKREEEEAAESQRLHASYACSRSLLLPYKSKNEQRISVISSTSSNTTSGIVSDRVHSEDELEIMINTPPAPLAAPSTESLALAHLLDRPSVSRQTSSVGQMSLKDLEEQLAALSVRPQDASSNGATIVTNSSVQRNSMGTTANDSSTATDSPSSQHNIGSQCSSTCSTVVVTSPVNGAGASSSGAPIPVHSTSSSLELGFSHTAQNSALSETSPDDFLSTSAREETESVSGASGVYTLAHGAPPTETSGVYTMHSSELTGQSSEIAESEKSSHYGMFQPQKLEETHVQHSDSVDGKKKEDFRPRSDSNVSTGSSFRGDGSDPTDNKHSLLSAEELTNLIVGRGTYPSRKTVSSSLHSDCDYVTLPLGDQGEEEVDQPPAPPPPYSARHEKTGLCGPPIAKPIPKPIAVVAPKPDSPPCSPPVPPAPIPAPPPAIRRRDPPPYSISSKPRPTSLISVSSSAHPAPSAAGSMSSLKSEEVTARFITTRPQISILKAHTSLIPDGAKPSYAAPHHCSSVASSNGSVCSHQLSQQSLHNSNYAGGSQASLHHHHVPSHHRHSGSAAIGIVPYGLHKSTASLHHQQSCVLLPVIKPRQFLAPPPPSLPRQPPPPPPPNHPHLASHLYEREMARKQLELYQQQLYSDVDYVIYPIQDPAVSQQEYLDAKQGSLLAAMAQAAPPPPHHPYLAMQVSPAIYRSTPYLPLTLSTHSRYASTQNLSDTYVQLPGPGYSPLYSPSMASLCSSYEPPPPPPLHPAALAAAAAAGAGSSSSSMFARSRSDDNILNSLDLLPKGKRLPPPPPPPYVNRRLKKPPMPAPSEKPPPIPSKPIPSRMSPIPPRKPPTLNPHHANSPLTKTSSGAQWAGERPRPDLGLGLGLNRGNNSILAQLQASMVAQSHAQAQAQALDIALLREKSKHLDLPLISALCNDRSLLKQTKVVINPKTGQEMPTSSAQPSGATTNGVANSSAGAGTLSKARKGSTVSHRHPQDKLPPLPVQQLAEANNYVIDPAVMMKQQQQQQQHNKTS

Target ORF information:

RefSeq Version	NM_001272894.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster expanded (ex), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272894.1

ORF Insert Sequence:

ATGCGAGCAT TTTGCACCGT CAGCGCGCCT TTGGAAGTAT GCGCGTCCTC AGCCGAGCAA 
CTATCACCAG GCTCGCGATT CCTGGCCCTG AGACTTCTGG GGCAGCAGCA GCCGAAAACC 
TTGTACTTCC TAGTCGATGC CAAGAGTCGA GTTCGAGAGG TTTACACGCA GACATGTCTG 
CACTTTGCCA CGCAAGGGAT GCTGGACACC GAACTCTTCG GTCTGGCTGT GCTCATAGAT 
GGCGAGTACA TGTTCGCAGA TCCCGAGAGC AAGCTCTCAA AATATGGCCC AAAGAGCTGG 
CGATCATCGC ACACCCACGG GCTGGATGCC AACGGACGAC CGCTCCTGGA GCTACACTTT 
CGCGTGCAGT TCTACATCGA GAGCCCGTTC ATGCTGAAAG ATGAGACGTC CCGCCATAAT 
TACTACCTGC AGCTGCGCCA CAACATCTTG CAGCGCGATT TGCCGAGAGA ACAGGCGGAG 
CAGGCACTGG TCTTCCTTGC TGGACTTGCT CTTCAGGCGG ACCTGGGTGA TGCTCCTCCG 
GGCACGAGTA ATTCCAAGGA TGATTCAGGA GAGGAGACAT CGGCATCTCC TTCTAACGGA 
GGAAGAGGTC TAAGTGCCAC CACTACATTA CCCAAGATCA GCAAACGTGC TAATGAGAGG 
ATGCTTAGAC TCTCGACATA TGTGGCGTCC ACATCAAAGA GAGAAACAAT ACCATTACCA 
CCCAGCCTGC CACCGAATGG AGCAGATTAC TACCGGATCG AGGATTACCT ACCCAGTGGA 
CTGCACACTC CCTGGGCGAG AAGTGCAATG AGAGCCTGTC ATCGGGAGCA TTTGGGCATG 
GCCACGGCAG AGGCGGAGCT GCTATATATT CAACAGGCAT GCAGTCTTCA TGAAACCATC 
AATGCCCATA CCTACAGGAT GCGATTGGCC AAAAGCGAGC AGGGATCTGG TAGTGCCTGG 
TTTGTGGTCT ATGCCAAGGG AATCAAGATT CTGGGAGGAG AGTCTACCAA CAGCTCTTCC 
AATCCCGAAA CCACAACCTT TCTGTGGCCG AATATCACGA AGCTCTCCTT CGAGCGGAAA 
AAGTTTGAAA TACGATCTGG AGAGAGCCGA ATCACCCTGT ACGCCGCATC CGATGAGAAA 
AATAAACTCC TATTGACCCT CTGCAAGGAT ACACATCAGT GGAGCATGAA ACTGGCTGCC 
AGGCTAAAGG AGGTATCCAA ACGCGAGGAG GAGGAGGCTG CCGAATCACA AAGGCTACAT 
GCCAGCTACG CTTGTTCCAG AAGTCTGCTC TTGCCCTACA AAAGTAAAAA CGAGCAGCGA 
ATATCGGTTA TCTCGAGTAC CAGCTCCAAC ACAACCTCTG GGATAGTAAG TGATCGAGTG 
CACTCCGAAG ACGAGCTGGA GATCATGATC AATACACCAC CAGCACCCTT AGCTGCTCCG 
TCAACGGAAA GTTTGGCCTT GGCTCATCTT TTGGACAGAC CCAGTGTGAG CAGACAAACT 
TCGAGCGTGG GTCAGATGTC TCTGAAGGAT TTGGAAGAAC AACTGGCTGC CTTGAGTGTG 
AGGCCACAAG ACGCGAGTTC TAACGGGGCC ACGATCGTCA CGAACTCCTC TGTTCAGAGA 
AATTCCATGG GAACAACTGC CAATGATTCA TCTACCGCCA CTGATTCACC CAGTTCGCAG 
CATAACATCG GCTCTCAGTG CTCATCCACT TGTAGCACTG TGGTGGTAAC ATCTCCTGTA 
AATGGAGCAG GCGCTTCCTC TTCAGGTGCT CCCATACCTG TGCACTCCAC TTCATCTAGT 
TTGGAGCTTG GCTTTAGTCA CACCGCTCAA AACTCGGCCT TGAGCGAAAC GAGTCCCGAC 
GATTTTCTCT CCACATCGGC AAGAGAAGAA ACGGAGAGTG TATCGGGCGC ATCTGGGGTT 
TATACTTTGG CGCACGGAGC TCCACCCACG GAAACCTCAG GCGTTTACAC CATGCACAGC 
AGTGAGCTAA CTGGACAATC ATCGGAGATC GCCGAGTCGG AAAAGAGTTC GCACTACGGG 
ATGTTCCAGC CCCAAAAACT AGAAGAAACC CACGTGCAAC ATTCTGATTC CGTAGATGGA 
AAAAAGAAAG AAGACTTCAG ACCGCGATCG GATTCTAACG TCAGCACAGG GAGTTCCTTT 
AGAGGCGATG GCAGCGATCC AACAGACAAC AAACATTCCT TGCTCTCCGC AGAAGAGTTA 
ACCAATTTGA TTGTGGGCAG GGGGACCTAC CCCAGTCGGA AAACAGTATC TAGTAGCCTT 
CACTCGGACT GTGATTACGT GACGTTACCA CTAGGAGATC AAGGAGAAGA GGAGGTAGAC 
CAACCACCAG CACCACCACC ACCCTACAGT GCTAGGCATG AGAAAACTGG TCTCTGTGGC 
CCACCTATCG CCAAACCTAT CCCTAAACCT ATAGCTGTGG TAGCTCCCAA ACCAGATTCA 
CCGCCATGTA GTCCACCGGT ACCGCCAGCA CCAATTCCAG CCCCACCGCC GGCTATTCGA 
CGAAGAGATC CACCGCCATA TTCCATCTCT AGCAAGCCTA GACCCACATC CCTGATATCT 
GTCAGTTCGT CGGCACATCC TGCGCCCAGT GCTGCCGGCT CGATGAGTTC TTTAAAGTCC 
GAGGAGGTTA CAGCCAGATT TATCACCACC AGGCCACAGA TTAGTATTCT GAAAGCCCAC 
ACCAGCCTGA TTCCCGATGG GGCCAAGCCA AGTTACGCGG CACCGCACCA TTGTTCATCA 
GTTGCCTCCT CCAATGGATC GGTTTGCAGC CATCAGTTGA GTCAGCAATC GCTGCATAAC 
TCCAACTATG CCGGCGGATC CCAGGCTTCA CTGCACCATC ATCACGTGCC GTCACACCAC 
CGACATTCTG GATCGGCAGC CATTGGAATA GTTCCTTACG GTCTGCACAA GAGCACGGCA 
TCACTGCATC ATCAGCAGTC ATGTGTACTA CTACCGGTTA TTAAACCTCG GCAGTTCCTG 
GCACCACCAC CGCCCAGTTT GCCGCGGCAG CCTCCACCAC CGCCCCCACC GAATCACCCC 
CATTTGGCGA GTCACCTTTA CGAGAGGGAA ATGGCTAGAA AACAATTGGA GCTCTACCAG 
CAGCAGCTTT ACAGCGATGT GGATTACGTA ATTTACCCCA TCCAGGATCC AGCTGTAAGC 
CAGCAAGAGT ATCTGGATGC CAAACAGGGA TCCTTACTGG CGGCAATGGC CCAAGCAGCA 
CCACCCCCAC CGCACCACCC ATATCTGGCC ATGCAGGTGT CGCCGGCGAT TTACAGAAGC 
ACCCCCTACC TACCACTGAC TCTGTCGACC CACTCACGAT ATGCATCCAC CCAAAATCTA 
TCGGATACAT ATGTGCAGCT TCCAGGACCC GGATACTCAC CCCTGTACTC TCCTTCGATG 
GCCAGCCTAT GCTCCTCGTA TGAGCCTCCA CCGCCTCCTC CCCTTCACCC GGCTGCTCTA 
GCAGCAGCTG CTGCGGCGGG GGCTGGCTCC TCGTCTTCCT CGATGTTTGC GCGATCGCGA 
TCAGATGACA ATATTCTGAA CTCACTAGAT TTGCTGCCCA AGGGAAAACG ACTGCCACCG 
CCGCCACCAC CACCGTATGT AAACAGACGG CTAAAGAAAC CACCCATGCC GGCGCCCAGT 
GAAAAGCCAC CTCCAATACC CTCTAAGCCA ATTCCTAGCA GGATGAGCCC GATTCCACCA 
CGAAAACCCC CAACGTTGAA TCCGCATCAT GCGAACTCAC CGCTAACAAA GACCTCCAGC 
GGCGCCCAGT GGGCAGGTGA GCGTCCGAGA CCTGACTTGG GTCTTGGTCT GGGATTGAAC 
CGGGGAAACA ACAGCATTTT GGCCCAGCTG CAGGCTTCAA TGGTAGCCCA GTCTCATGCC 
CAGGCACAGG CCCAAGCGCT AGACATTGCG CTTCTTCGGG AGAAGAGCAA GCATCTAGAT 
CTGCCGCTGA TCTCGGCGCT CTGCAACGAT CGCTCCTTGC TGAAACAGAC TAAGGTTGTT 
ATAAATCCGA AGACTGGCCA GGAGATGCCC ACTTCCAGTG CGCAACCTTC AGGTGCCACC 
ACAAATGGAG TTGCCAACTC CTCTGCAGGA GCAGGAACCC TAAGCAAGGC CAGAAAAGGA 
TCTACCGTAA GCCACCGTCA TCCGCAGGAC AAACTGCCGC CTTTACCTGT CCAACAATTG 
GCGGAGGCCA ACAACTACGT GATCGATCCC GCTGTCATGA TGAAGCAACA GCAGCAGCAG 
CAGCAGCACA ACAAGACCAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ex cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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