ebi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ebi
Entrez Gene ID	33212
Full Name	CG4063 gene product from transcript CG4063-RA
Synonyms	CG4063,Dmel\CG4063,E-2f,E-2g,Ebi,FBXW4,Tb11,Tbl1,l(2)k16213
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21C2-21C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057981.4	NP_477329.1	ebi

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TBL1X	XP_001140707.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007739	XP_317781.4
Xenopus tropicalis (tropical clawed frog)	tbl1x	NP_001120367.1
Danio rerio (zebrafish)	tbl1x	NP_001076463.1
Drosophila melanogaster (fruit fly)	ebi	NP_477329.1
Mus musculus (house mouse)	Tbl1x	NP_065626.1
Rattus norvegicus (Norway rat)	Tbl1x	NP_001100434.1
Canis lupus familiaris (dog)	TBL1X	XP_005641150.1
Homo sapiens (human)	TBL1X	NP_005638.1
Bos taurus (cattle)	TBL1X	NP_001138704.1
Gallus gallus (chicken)	TBL1X	NP_001153226.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study proposes that the interplay of Crb expression in the DV boundary and ubiquitin-dependent Crb downregulation by Ebi provides a mechanism for the maintenance of Notch signaling during wing development.
Title: Ebi modulates wing growth by ubiquitin-dependent downregulation of Crumbs in Drosophila.

Results offer clear in vivo evidence that the Ebi-containing co-repressor complex acts in a distinct manner to regulate transcription that is required for modulating the output of various processes during Drosophila development.
Title: Cellular Defense and Sensory Cell Survival Require Distinct Functions of ebi in Drosophila.

The antagonistic function of ebi against the Polycomb group silencing complex plays a role in the G1/S phase transition.
Title: Ebi alleviates excessive growth signaling through multiple epigenetic functions in Drosophila.

Ebi/AP-1 suppresses basal transcription levels of apoptotic genes and thereby protects sensory neurons from degeneration
Title: Ebi/AP-1 suppresses pro-apoptotic genes expression and permits long-term survival of Drosophila sensory neurons.

EBI acts to activate (de-repress) transcription of Notch target genes important for Drosophila wing growth by functioning as a corepressor/coactivator exchange factor for SU(H).
Title: Genetic characterization of ebi reveals its critical role in Drosophila wing growth.

The following ebi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ebi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13343	NM_057981.4 Latest version!	Drosophila melanogaster ebi (ebi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ebi ( NM_057981.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477329.1 Drosophila melanogaster ebi (ebi), mRNA.

Price & Availability↑

CloneID	OFa13343
Clone ID Related Accession (Same CDS sequence)	NM_057981.4
Accession Version	NM_057981.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ebi
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057981.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTTTT CCAGCGACGA GGTGAACTTT CTGGTTTACC GATACCTGCA GGAGTCGGGC 
TTTCTGCACT CGGCCTACGT GTTTGGCATT GAGTCACACA TCTCGCAAAG TAATATCAAT 
GGCGCATTGG TTCCACCTGC CGCTTTGCTC ACCATCCTGC AGAAGGGTCT TCTGTACACT 
GAAGTGGAGT GGAGCGTAGG CGAAGACGGC GAGGTGGCGC GACCCATTGA AGGACTCTCT 
CTAATAGACG CTGTCATGCC GGAGGTGAAG CCACTCAAGC CCATTGTGAA AACAGAGCCG 
GGAAAACCCG GGGCAGTCGA CTCTTCTGCC CCTGCCGGTG GCAACCAGAA CAACAATGCC 
AAGCCTGAGA TAAAAATAGA ACCGGGTACC GGAGTGGCGG GCTCAGCAGG TGGAAACAAA 
ATTGCTGGCA GCACCACGGG CACAAGCACA CCAACCGATC AGTCGGCAAG CGAGGTAGAC 
TCCAGTGGAA ATGCGGCAAA TAATGCTGGC GGCACATACG CCGGAAACAA TGGAGCTGGA 
GGGAACCAGG CCAGTACTGG AGGATCTAAT AGCACAAGCA CACCAGCTGG TGGAGATTTG 
GCAGCACCTG GGGCAAGTCA AAAGAAATCC CAGAACTCGA ATGAAGCGGG AAGCTCATCT 
AGTGGCAATG CAGGCAATGC AAATGCTACC AGTACCGATG ATGCAGCCTC CTCAACATCC 
ACAAATGGCA ATAGCAGCAC TTCCAGCAGC GTAGAACAAC CTACAAGCGG GCTGACGCCC 
GCTGGTGGAA CTGTATCAAC CTCCAATCCG GACGCAGCAG CATCGGGCGG AGCTTCGACT 
GCAACAGGAT CAAAGGCGCC AAGTGGAGCG GTTACGATTC GCGTAGGAGC CCAAGGAAAC 
AACGTGCAGT CTGGTTCGTC CAATGCTCAA AGCTCTGCTC CCAGTGGAAC CATCTCCTCG 
TCTACCAGCG GAGGTGCTGG GACTCCCGCA GCTCTTGTTC CCATGGACAT TGACGAGAAC 
ATCGAAATAC CCGAATCCAA GGCTCGTGTA TTACGCGGCC ACGAAAGCGA GGTGTTTATC 
TGCGCATGGA ATCCTAGCCG GGATTTACTG GCAAGCGGTT CGGGAGACAG CACTGCTAGG 
ATATGGGACA TGTCCGATGC AAACACTAAC TCCAACCAGT TGGTGCTGCG CCACTGCATC 
CAGAAAGGCG GCGCTGAGGT GCCTAGCAAC AAGGACGTCA CCTCTCTGGA CTGGAATTGC 
GATGGTTCCT TGCTCGCCAC CGGCAGCTAC GATGGTTACG CCCGAATTTG GAAAACCGAC 
GGTCGACTTG CTTCTACTTT GGGACAACAC AAGGGTCCGA TCTTTGCTCT GAAGTGGAAC 
AAGTGCGGCA ACTACATCCT CTCGGCTGGC GTGGACAAGA CGACGATCAT CTGGGACGCA 
TCCACGGGCC AATGCACCCA GCAATTTGCC TTTCACAGTG CTCCAGCCTT GGATGTGGAC 
TGGCAGACAA ACCAGGCCTT TGCCTCTTGC AGTACGGATC AGCGGATACA TGTGTGCCGG 
TTGGGTGTAA ATGAACCCAT CAAGACCTTT AAAGGACACA CAAACGAGGT GAATGCAATC 
AAGTGGTGTC CGCAGGGCCA ACTGCTGGCG TCCTGCTCTG ATGACATGAC CCTCAAGATC 
TGGAGCATGA ACCGAGATCG TTGCTGCCAC GATCTGCAGG CTCACTCAAA GGAGATCTAT 
ACGATAAAAT GGTCGCCCAC GGGGCCGGGA ACCAATAATC CAAACACAAA CCTGATCCTC 
GCATCCGCTT CGTTCGATTC CACGGTAAGA CTGTGGGACG TGGAGAGGGG CAGCTGTATC 
CACACGCTCA CCAAGCACAC AGAGCCAGTC TATTCGGTGG CCTTCAGTCC GGATGGAAAG 
CATTTGGCCT CCGGCAGCTT CGACAAGTGC GTACACATTT GGAGCACGCA AACGGGACAG 
CTAGTGCACA GTTACAAGGG CACGGGCGGA ATCTTTGAGG TGTGCTGGAA CTCCAAGGGC 
ACTAAGGTGG GTGCCAGTGC CTCCGACGGT AGCGTTTTCG TGTTGGACCT GCGAAAGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477329.1
CDS	323..2425
Translation	MSFSSDEVNFLVYRYLQESGFLHSAYVFGIESHISQSNINGALVPPAALLTILQKGLLYTEVEWSVGEDGEVARPIEGLSLIDAVMPEVKPLKPIVKTEPGKPGAVDSSAPAGGNQNNNAKPEIKIEPGTGVAGSAGGNKIAGSTTGTSTPTDQSASEVDSSGNAANNAGGTYAGNNGAGGNQASTGGSNSTSTPAGGDLAAPGASQKKSQNSNEAGSSSSGNAGNANATSTDDAASSTSTNGNSSTSSSVEQPTSGLTPAGGTVSTSNPDAAASGGASTATGSKAPSGAVTIRVGAQGNNVQSGSSNAQSSAPSGTISSSTSGGAGTPAALVPMDIDENIEIPESKARVLRGHESEVFICAWNPSRDLLASGSGDSTARIWDMSDANTNSNQLVLRHCIQKGGAEVPSNKDVTSLDWNCDGSLLATGSYDGYARIWKTDGRLASTLGQHKGPIFALKWNKCGNYILSAGVDKTTIIWDASTGQCTQQFAFHSAPALDVDWQTNQAFASCSTDQRIHVCRLGVNEPIKTFKGHTNEVNAIKWCPQGQLLASCSDDMTLKIWSMNRDRCCHDLQAHSKEIYTIKWSPTGPGTNNPNTNLILASASFDSTVRLWDVERGSCIHTLTKHTEPVYSVAFSPDGKHLASGSFDKCVHIWSTQTGQLVHSYKGTGGIFEVCWNSKGTKVGASASDGSVFVLDLRKF

Target ORF information:

RefSeq Version	NM_057981.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ebi (ebi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057981.4

ORF Insert Sequence:

ATGAGTTTTT CCAGCGACGA GGTGAACTTT CTGGTTTACC GATACCTGCA GGAGTCGGGC 
TTTCTGCACT CGGCCTACGT GTTTGGCATT GAGTCACACA TCTCGCAAAG TAATATCAAT 
GGCGCATTGG TTCCACCTGC CGCTTTGCTC ACCATCCTGC AGAAGGGTCT TCTGTACACT 
GAAGTGGAGT GGAGCGTAGG CGAAGACGGC GAGGTGGCGC GACCCATTGA AGGACTCTCT 
CTAATAGACG CTGTCATGCC GGAGGTGAAG CCACTCAAGC CCATTGTGAA AACAGAGCCG 
GGAAAACCCG GGGCAGTCGA CTCTTCTGCC CCTGCCGGTG GCAACCAGAA CAACAATGCC 
AAGCCTGAGA TAAAAATAGA ACCGGGTACC GGAGTGGCGG GCTCAGCAGG TGGAAACAAA 
ATTGCTGGCA GCACCACGGG CACAAGCACA CCAACCGATC AGTCGGCAAG CGAGGTAGAC 
TCCAGTGGAA ATGCGGCAAA TAATGCTGGC GGCACATACG CCGGAAACAA TGGAGCTGGA 
GGGAACCAGG CCAGTACTGG AGGATCTAAT AGCACAAGCA CACCAGCTGG TGGAGATTTG 
GCAGCACCTG GGGCAAGTCA AAAGAAATCC CAGAACTCGA ATGAAGCGGG AAGCTCATCT 
AGTGGCAATG CAGGCAATGC AAATGCTACC AGTACCGATG ATGCAGCCTC CTCAACATCC 
ACAAATGGCA ATAGCAGCAC TTCCAGCAGC GTAGAACAAC CTACAAGCGG GCTGACGCCC 
GCTGGTGGAA CTGTATCAAC CTCCAATCCG GACGCAGCAG CATCGGGCGG AGCTTCGACT 
GCAACAGGAT CAAAGGCGCC AAGTGGAGCG GTTACGATTC GCGTAGGAGC CCAAGGAAAC 
AACGTGCAGT CTGGTTCGTC CAATGCTCAA AGCTCTGCTC CCAGTGGAAC CATCTCCTCG 
TCTACCAGCG GAGGTGCTGG GACTCCCGCA GCTCTTGTTC CCATGGACAT TGACGAGAAC 
ATCGAAATAC CCGAATCCAA GGCTCGTGTA TTACGCGGCC ACGAAAGCGA GGTGTTTATC 
TGCGCATGGA ATCCTAGCCG GGATTTACTG GCAAGCGGTT CGGGAGACAG CACTGCTAGG 
ATATGGGACA TGTCCGATGC AAACACTAAC TCCAACCAGT TGGTGCTGCG CCACTGCATC 
CAGAAAGGCG GCGCTGAGGT GCCTAGCAAC AAGGACGTCA CCTCTCTGGA CTGGAATTGC 
GATGGTTCCT TGCTCGCCAC CGGCAGCTAC GATGGTTACG CCCGAATTTG GAAAACCGAC 
GGTCGACTTG CTTCTACTTT GGGACAACAC AAGGGTCCGA TCTTTGCTCT GAAGTGGAAC 
AAGTGCGGCA ACTACATCCT CTCGGCTGGC GTGGACAAGA CGACGATCAT CTGGGACGCA 
TCCACGGGCC AATGCACCCA GCAATTTGCC TTTCACAGTG CTCCAGCCTT GGATGTGGAC 
TGGCAGACAA ACCAGGCCTT TGCCTCTTGC AGTACGGATC AGCGGATACA TGTGTGCCGG 
TTGGGTGTAA ATGAACCCAT CAAGACCTTT AAAGGACACA CAAACGAGGT GAATGCAATC 
AAGTGGTGTC CGCAGGGCCA ACTGCTGGCG TCCTGCTCTG ATGACATGAC CCTCAAGATC 
TGGAGCATGA ACCGAGATCG TTGCTGCCAC GATCTGCAGG CTCACTCAAA GGAGATCTAT 
ACGATAAAAT GGTCGCCCAC GGGGCCGGGA ACCAATAATC CAAACACAAA CCTGATCCTC 
GCATCCGCTT CGTTCGATTC CACGGTAAGA CTGTGGGACG TGGAGAGGGG CAGCTGTATC 
CACACGCTCA CCAAGCACAC AGAGCCAGTC TATTCGGTGG CCTTCAGTCC GGATGGAAAG 
CATTTGGCCT CCGGCAGCTT CGACAAGTGC GTACACATTT GGAGCACGCA AACGGGACAG 
CTAGTGCACA GTTACAAGGG CACGGGCGGA ATCTTTGAGG TGTGCTGGAA CTCCAAGGGC 
ACTAAGGTGG GTGCCAGTGC CTCCGACGGT AGCGTTTTCG TGTTGGACCT GCGAAAGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ebi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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