l(1)G0196 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following l(1)G0196 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(1)G0196 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa02274 NM_176778.2
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa21571 NM_001298605.1
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa02271 NM_176777.2
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa02272 NM_001103571.2
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa02273 NM_176780.2
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa02275 NM_176779.3
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa21569 NM_001298606.1
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant O, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa21570 NM_001298607.1
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant P, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa21572 NM_001298604.1
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa21573 NM_001298603.1
Latest version!
Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa02274
    Clone ID Related Accession (Same CDS sequence) NM_176778.2
    Accession Version NM_176778.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 26, 2009 this sequence version replaced NM_176778.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GATAGCCAAG TCTCGGTGTC GGTCTCGGCA
    TCGGTGTCAT CGGCCAACGC ATCCACGTCG TCGCGTCGCC AAAGACACAG TATTGCCGGC
    CAGATGTCCT ATATGAAAAT GTTGGGTTTC GGTGGTTTTA GCAAAAAGAT GGCCACCAGC
    GCAAATAGCC TTTTCAGCAC CGCCGTCATC AGCGGCAGCT CGTCCGCTCC AAATCTTCGC
    GACATGATAA CGGTCTCCTC TTCCGGATTT GGCGATGTAC CACCAATCCG CCCGCTGGAG
    ACACTGCACA ACGCCCTTTC GCTGCGCAAG CTGGACAGTT TCTTGCAGGA CATGATCCTG
    GCGCAGATCT TTAAGACGCC GACAGGGTCC CCGCCGCGGG GCTTCTCTAA GAATACCTTG
    CCAGCGGTTT CCTCGATGAC TCTAACCGCC TCAAATCAGA CAGAGGTTGT CGAACATGAG
    CCGATATCAA CGACCGTAAC GCCCACCTTT GACCGGCGTG GCAGCGAGTC CGGATCCACA
    GCGGATGCCC ATCTGCGTCT TCTTTCGGAG AGCCAGTGCC CTAATCTGGA CGATAGCAAC
    ACCGAGCTGC GAGAATCGCT GGCTGGAAAA ATGGAGCAAT ATCCAACAGA GCCCAGCATT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788951.2
    CDS488..4390
    Translation

    Target ORF information:

    RefSeq Version NM_176778.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176778.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GATAGCCAAG TCTCGGTGTC GGTCTCGGCA
    TCGGTGTCAT CGGCCAACGC ATCCACGTCG TCGCGTCGCC AAAGACACAG TATTGCCGGC
    CAGATGTCCT ATATGAAAAT GTTGGGTTTC GGTGGTTTTA GCAAAAAGAT GGCCACCAGC
    GCAAATAGCC TTTTCAGCAC CGCCGTCATC AGCGGCAGCT CGTCCGCTCC AAATCTTCGC
    GACATGATAA CGGTCTCCTC TTCCGGATTT GGCGATGTAC CACCAATCCG CCCGCTGGAG
    ACACTGCACA ACGCCCTTTC GCTGCGCAAG CTGGACAGTT TCTTGCAGGA CATGATCCTG
    GCGCAGATCT TTAAGACGCC GACAGGGTCC CCGCCGCGGG GCTTCTCTAA GAATACCTTG
    CCAGCGGTTT CCTCGATGAC TCTAACCGCC TCAAATCAGA CAGAGGTTGT CGAACATGAG
    CCGATATCAA CGACCGTAAC GCCCACCTTT GACCGGCGTG GCAGCGAGTC CGGATCCACA
    GCGGATGCCC ATCTGCGTCT TCTTTCGGAG AGCCAGTGCC CTAATCTGGA CGATAGCAAC
    ACCGAGCTGC GAGAATCGCT GGCTGGAAAA ATGGAGCAAT ATCCAACAGA GCCCAGCATT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21571
    Clone ID Related Accession (Same CDS sequence) NM_001298605.1
    Accession Version NM_001298605.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GCTTTCCTGA ATAATTGCGC GTTCCTCTTT
    TGTCTTACAC ATCTCACAAC CACACGATTG GCTATTCCCA GTTTTAGACT AATCCGCTCT
    GATTCGGTTT GGTTTTTCCT AATTAAACAT TGTTTGTTTT CTATCACAAT TCGACAATGT
    AATAATGACA TTGGATATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285534.1
    CDS488..3928
    Translation

    Target ORF information:

    RefSeq Version NM_001298605.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298605.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GCTTTCCTGA ATAATTGCGC GTTCCTCTTT
    TGTCTTACAC ATCTCACAAC CACACGATTG GCTATTCCCA GTTTTAGACT AATCCGCTCT
    GATTCGGTTT GGTTTTTCCT AATTAAACAT TGTTTGTTTT CTATCACAAT TCGACAATGT
    AATAATGACA TTGGATATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02271
    Clone ID Related Accession (Same CDS sequence) NM_176777.2
    Accession Version NM_176777.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 26, 2009 this sequence version replaced NM_176777.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GATAGCCAAG TCTCGGTGTC GGTCTCGGCA
    TCGGTGTCAT CGGCCAACGC ATCCACGTCG TCGCGTCGCC AAAGACACAG TATTGCCGGC
    CAGATGTCCT ATATGAAAAT GTTGGGTTTC GGTGGTTTTA GCAAAAAGAT GGCCACCAGC
    GCAAATAGCC TTTTCAGCAC CGCCGTCATC AGCGGCAGCT CGTCCGCTCC AAATCTTCGC
    GACATGATAA CGGTCTCCTC TTCCGGATTT GGCGATGTAC CACCAATCCG CCCGCTGGAG
    ACACTGCACA ACGCCCTTTC GCTGCGCAAG CTGGACAGTT TCTTGCAGGA CATGATCCTG
    GCGCAGATCT TTAAGACGCC GACAGGGTCC CCGCCGCGGG GCTTCTCTAA GAATACCTTG
    CCAGCGGTTT CCTCGATGAC TCTAACCGCC TCAAATCAGA CAGAGGTTGT CGAACATGAG
    CCGATATCAA CGACCGTAAC GCCCACCTTT GACCGGCGTG GCAGCGAGTC CGGATCCACA
    GCGGATGCCC ATCTGCGTCT TCTTTCGGAG AGCCAGTGCC CTAATCTGGA CGATAGCAAC
    ACCGAGCTGC GAGAATCGCT GGCTGGAAAA ATGGAGCTTT GGCCACAGGA CATTGTGAAG
    GCAGCCGAGG ATGAGGAATT AAACCTGTCA GAATTGGAAA CAAAACCAAG CTTGGACCTT
    ACCATGGAGA TTATGGAGCG CGGGGTCGCG GGTATCGCCT CCATCCAGCC AATGAATCGG
    GACAGCGATG AAACGATGGG CGGTGGAGTC TTTCTGAGTG TTTGTGAGGA ACAGGGCAGC
    GGAAGCACCT GCCTTACACC AGTTAGTTTT GGGATGGATC TCGACCTGAG CATGGTGGCT
    AACAAGGGCT CCATTACGCT GTCAATGGAA GGGTTCGATG ATGATGAAGA TGCCACTTTG
    TCGGCGGCCA CAACACCTTC CCTTTTGGCG GACTGTGAGC CGCGCCTGGA GAGTTGCTAT
    TGTTGCCCCA GCCATGCAGA GGCCCCGCCT GAGGTTCCCA GCGATGATCC ACGGTTTGGC
    TTTGCGCTGC CCGTTCGAGT CACACAAGCT TCACCGGAGC ATGCGCGACC TGTGCGCCGT
    GCTCATGATC CCGTCTCGCC AAGGATCCAG AAACAGATCA GCTTGTTCGA GGGAAACGCG
    GCTATGGCAA CGGGTCAGGA AAAGACTGAG TCCAGCGGGT CGGTTGGGGG CGGTGCAATT
    CTGCACGCCT CCATTAACCT GCCGGCGGCG GGACCGCATC ATTTGCGCCA AGATGCTCGC
    CTGCGCAAGT TTGAGAACCT CACGCAGTCC ACATCAAACT CGAACTTTCC CTTCGAAAGC
    AACACGCTGA AGCGGGTGCC CATGCAGACC ACCAAAGACT ACGACAACGT GAGCCACACA
    CAGAGCTGCA TAAATCTCAA AAGTGGCAGC AGTGGAGTCC TCGGGGGATC GCCGCAGCGT
    CAGAGAGGAT CCGATGGAGG AGGCGTAGGT GCAAGTGGAG TCCCGGCAGA GAGCCGGGAG
    CCGACGCGGG TGCATTATGG ACGGATGAAC AGTACATGCT GCTCTGCCTC GGCATCGCCG
    TCCCCCTCCC CGGGGGCGCT GATTGTCAAG GAGCGGTTCA TCGAACCGCC CAAGCGCGGT
    GTCGTGCGCG GATACCATGG CAAAACGCAG TCCATGGACG CCGACTTCCT GTTCAACGAG
    TTTCTACTGC TGCCGGCCAT GGCGCCCGCC AAGATATCCT TTGATAGTTC CGATATAGAC
    CAGGCCAGCG ATGACGACTC CCCGAGCAGC TCGAAACAAA GGCACGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788950.2
    CDS488..5578
    Translation

    Target ORF information:

    RefSeq Version NM_176777.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176777.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GATAGCCAAG TCTCGGTGTC GGTCTCGGCA
    TCGGTGTCAT CGGCCAACGC ATCCACGTCG TCGCGTCGCC AAAGACACAG TATTGCCGGC
    CAGATGTCCT ATATGAAAAT GTTGGGTTTC GGTGGTTTTA GCAAAAAGAT GGCCACCAGC
    GCAAATAGCC TTTTCAGCAC CGCCGTCATC AGCGGCAGCT CGTCCGCTCC AAATCTTCGC
    GACATGATAA CGGTCTCCTC TTCCGGATTT GGCGATGTAC CACCAATCCG CCCGCTGGAG
    ACACTGCACA ACGCCCTTTC GCTGCGCAAG CTGGACAGTT TCTTGCAGGA CATGATCCTG
    GCGCAGATCT TTAAGACGCC GACAGGGTCC CCGCCGCGGG GCTTCTCTAA GAATACCTTG
    CCAGCGGTTT CCTCGATGAC TCTAACCGCC TCAAATCAGA CAGAGGTTGT CGAACATGAG
    CCGATATCAA CGACCGTAAC GCCCACCTTT GACCGGCGTG GCAGCGAGTC CGGATCCACA
    GCGGATGCCC ATCTGCGTCT TCTTTCGGAG AGCCAGTGCC CTAATCTGGA CGATAGCAAC
    ACCGAGCTGC GAGAATCGCT GGCTGGAAAA ATGGAGCTTT GGCCACAGGA CATTGTGAAG
    GCAGCCGAGG ATGAGGAATT AAACCTGTCA GAATTGGAAA CAAAACCAAG CTTGGACCTT
    ACCATGGAGA TTATGGAGCG CGGGGTCGCG GGTATCGCCT CCATCCAGCC AATGAATCGG
    GACAGCGATG AAACGATGGG CGGTGGAGTC TTTCTGAGTG TTTGTGAGGA ACAGGGCAGC
    GGAAGCACCT GCCTTACACC AGTTAGTTTT GGGATGGATC TCGACCTGAG CATGGTGGCT
    AACAAGGGCT CCATTACGCT GTCAATGGAA GGGTTCGATG ATGATGAAGA TGCCACTTTG
    TCGGCGGCCA CAACACCTTC CCTTTTGGCG GACTGTGAGC CGCGCCTGGA GAGTTGCTAT
    TGTTGCCCCA GCCATGCAGA GGCCCCGCCT GAGGTTCCCA GCGATGATCC ACGGTTTGGC
    TTTGCGCTGC CCGTTCGAGT CACACAAGCT TCACCGGAGC ATGCGCGACC TGTGCGCCGT
    GCTCATGATC CCGTCTCGCC AAGGATCCAG AAACAGATCA GCTTGTTCGA GGGAAACGCG
    GCTATGGCAA CGGGTCAGGA AAAGACTGAG TCCAGCGGGT CGGTTGGGGG CGGTGCAATT
    CTGCACGCCT CCATTAACCT GCCGGCGGCG GGACCGCATC ATTTGCGCCA AGATGCTCGC
    CTGCGCAAGT TTGAGAACCT CACGCAGTCC ACATCAAACT CGAACTTTCC CTTCGAAAGC
    AACACGCTGA AGCGGGTGCC CATGCAGACC ACCAAAGACT ACGACAACGT GAGCCACACA
    CAGAGCTGCA TAAATCTCAA AAGTGGCAGC AGTGGAGTCC TCGGGGGATC GCCGCAGCGT
    CAGAGAGGAT CCGATGGAGG AGGCGTAGGT GCAAGTGGAG TCCCGGCAGA GAGCCGGGAG
    CCGACGCGGG TGCATTATGG ACGGATGAAC AGTACATGCT GCTCTGCCTC GGCATCGCCG
    TCCCCCTCCC CGGGGGCGCT GATTGTCAAG GAGCGGTTCA TCGAACCGCC CAAGCGCGGT
    GTCGTGCGCG GATACCATGG CAAAACGCAG TCCATGGACG CCGACTTCCT GTTCAACGAG
    TTTCTACTGC TGCCGGCCAT GGCGCCCGCC AAGATATCCT TTGATAGTTC CGATATAGAC
    CAGGCCAGCG ATGACGACTC CCCGAGCAGC TCGAAACAAA GGCACGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02272
    Clone ID Related Accession (Same CDS sequence) NM_001103571.2
    Accession Version NM_001103571.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 26, 2009 this sequence version replaced NM_001103571.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GTTTCTTCTC CTGATTTCGG GGATAATTCT
    CGGACTCGTT CTTCTGAATT CGGGGAGACT TGTCATGCTC GAGTGGGCAC TGCTAGTAAC
    TCAGATGGGG GCGGGTCTCA TCCGAGAGCG GATCATCGCA CTGCCTTTCA GATTCGTGTT
    ACAAATAGTT TGTCCTTCTT TAAAATTGAC TCTTCGACAA ACGAGCTGCC TTTGTCGGAC
    ATTGATTTTT CGCTGCACCC ACCCACTCCG CAATGCGGTC CCTTGTCGCA CAAGCGCTTC
    CACATCTTGA CTATGCGGCG GATGGAGAGT TGCGACGATG GTGCAGAACT CGAACAGGTT
    CGTCATTTGC CACAGATCTC GCCGATGGCC ACCAATGAGC GACCCTTAAG TTCCTGCAAT
    TGCAGTAGCT CAGCGGCCCA AGCGCATTCG CACTCAAAAA GTCTGATGGA CTTGGCACAA
    GCGGTGGTTA TGACTTCACC GCAAGAAACT GGACCGAATT CGGAGCAAGG TTGTGACCCA
    ACAACGGCGG CTGATGTGAG TGTTAGTAGT TTCGATGATG ACTTTCAGTT GTCTAGCTCA
    GCACCGGCCA TCCTGATGAG CGCACACTTC GGCAATAGGC CTGTGGTGGC GTCTCTATCG
    TCAATGGTCC ATGTCACTAC CTCGCCCTCT GCCTCTACTC TACAGCTCTG TCGGGACAAG
    GACAAGGCCC TGGCTTCTGG CACATCCTCT GCACACAGCA AAGCCACTAG TAACTCCTGT
    GGTCAGCTCT CCATGGCAGG TTCTGCTCCT GTGGTGACAG AAAACCCATT TCGATTCACC
    GTTTCGTCAT TGGGCTCTGC AGCTACCAAT ACCGCCTGTT TTGTAGGAAG CTTCGAGCCC
    ATTGAAGAGC AGATCACGTC CATTGTGGAG GTAGATTCAA AGCCCCTGCA GCCAGAGCCT
    CAAGTAGTAT ATAATCTGCC CACTGTGCTC ATTACGGGAA CAGCAAGTAG CTCAGAATTG
    TCGACCAAAC TAAATAGCAA TATACTACCG ACTGTTACAA ACGCTTTTTC TGCAATAGAT
    ACCGAAATAA ATGACGAAAT AGGTATATCG AAGGAAGTAG GAATAGGGAC AATAAGAATT
    ACAAACACAC ATACACCCTG CAATAAGGCA ACAGTTACTC GACCAGATAC AAAAATAGCT
    ATAACAACTG ATCCACAAAC GGTTACAGCA ACAGAGACAG CAGAAGATAC ATCAATGGAT
    ATACAGCCAA AGATATGTAT TGGAAAATGT GCACCTGGGC TGCTTACACC AACAGTTACT
    ACTACCGATA TTTTAAAAGG TATACGAGAC ATCGCTCCTA CAGCCACCCA AACATCCCCA
    CCAACATTTA CACCATCAAC CACTACTACA ACTGGAGCAG CAGCAGCTTT GTTTGATAAT
    ACAGCAACAA TGTCTTCTAA TCGACCATTT ACTAACCAAT TCCAATCGAT TGATCCCACT
    TCAAACGACG CACCACACCA ACATCATGTA CATCAACACC AATACCAACA TCACCGAAAA
    ACAACAACGA CCATCATCAA TAACACTCTG AACGAAAACC ACACCACCGC CACAATAACA
    AACAACACTA CCACGACCCC GTCTTGTTGT ACCAAAACCA TCGCCACAGA TAGCCAAGTC
    TCGGTGTCGG TCTCGGCATC GGTGTCATCG GCCAACGCAT CCACGTCGTC GCGTCGCCAA
    AGACACAGTA TTGCCGGCCA GATGTCCTAT ATGAAAATGT TGGGTTTCGG TGGTTTTAGC
    AAAAAGATGG CCACCAGCGC AAATAGCCTT TTCAGCACCG CCGTCATCAG CGGCAGCTCG
    TCCGCTCCAA ATCTTCGCGA CATGATAACG GTCTCCTCTT CCGGATTTGG CGATGTACCA
    CCAATCCGCC CGCTGGAGAC ACTGCACAAC GCCCTTTCGC TGCGCAAGCT GGACAGTTTC
    TTGCAGGACA TGATCCTGGC GCAGATCTTT AAGACGCCGA CAGGGTCCCC GCCGCGGGGC
    TTCTCTAAGA ATACCTTGCC AGCGGTTTCC TCGATGACTC TAACCGCCTC AAATCAGACA
    GAGGTTGTCG AACATGAGCC GATATCAACG ACCGTAACGC CCACCTTTGA CCGGCGTGGC
    AGCGAGTCCG GATCCACAGC GGATGCCCAT CTGCGTCTTC TTTCGGAGAG CCAGTGCCCT
    AATCTGGACG ATAGCAACAC CGAGCTGCGA GAATCGCTGG CTGGAAAAAT GGAGCAATAT
    CCAACAGAGC CCAGCATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097041.2
    CDS488..6028
    Translation

    Target ORF information:

    RefSeq Version NM_001103571.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103571.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GTTTCTTCTC CTGATTTCGG GGATAATTCT
    CGGACTCGTT CTTCTGAATT CGGGGAGACT TGTCATGCTC GAGTGGGCAC TGCTAGTAAC
    TCAGATGGGG GCGGGTCTCA TCCGAGAGCG GATCATCGCA CTGCCTTTCA GATTCGTGTT
    ACAAATAGTT TGTCCTTCTT TAAAATTGAC TCTTCGACAA ACGAGCTGCC TTTGTCGGAC
    ATTGATTTTT CGCTGCACCC ACCCACTCCG CAATGCGGTC CCTTGTCGCA CAAGCGCTTC
    CACATCTTGA CTATGCGGCG GATGGAGAGT TGCGACGATG GTGCAGAACT CGAACAGGTT
    CGTCATTTGC CACAGATCTC GCCGATGGCC ACCAATGAGC GACCCTTAAG TTCCTGCAAT
    TGCAGTAGCT CAGCGGCCCA AGCGCATTCG CACTCAAAAA GTCTGATGGA CTTGGCACAA
    GCGGTGGTTA TGACTTCACC GCAAGAAACT GGACCGAATT CGGAGCAAGG TTGTGACCCA
    ACAACGGCGG CTGATGTGAG TGTTAGTAGT TTCGATGATG ACTTTCAGTT GTCTAGCTCA
    GCACCGGCCA TCCTGATGAG CGCACACTTC GGCAATAGGC CTGTGGTGGC GTCTCTATCG
    TCAATGGTCC ATGTCACTAC CTCGCCCTCT GCCTCTACTC TACAGCTCTG TCGGGACAAG
    GACAAGGCCC TGGCTTCTGG CACATCCTCT GCACACAGCA AAGCCACTAG TAACTCCTGT
    GGTCAGCTCT CCATGGCAGG TTCTGCTCCT GTGGTGACAG AAAACCCATT TCGATTCACC
    GTTTCGTCAT TGGGCTCTGC AGCTACCAAT ACCGCCTGTT TTGTAGGAAG CTTCGAGCCC
    ATTGAAGAGC AGATCACGTC CATTGTGGAG GTAGATTCAA AGCCCCTGCA GCCAGAGCCT
    CAAGTAGTAT ATAATCTGCC CACTGTGCTC ATTACGGGAA CAGCAAGTAG CTCAGAATTG
    TCGACCAAAC TAAATAGCAA TATACTACCG ACTGTTACAA ACGCTTTTTC TGCAATAGAT
    ACCGAAATAA ATGACGAAAT AGGTATATCG AAGGAAGTAG GAATAGGGAC AATAAGAATT
    ACAAACACAC ATACACCCTG CAATAAGGCA ACAGTTACTC GACCAGATAC AAAAATAGCT
    ATAACAACTG ATCCACAAAC GGTTACAGCA ACAGAGACAG CAGAAGATAC ATCAATGGAT
    ATACAGCCAA AGATATGTAT TGGAAAATGT GCACCTGGGC TGCTTACACC AACAGTTACT
    ACTACCGATA TTTTAAAAGG TATACGAGAC ATCGCTCCTA CAGCCACCCA AACATCCCCA
    CCAACATTTA CACCATCAAC CACTACTACA ACTGGAGCAG CAGCAGCTTT GTTTGATAAT
    ACAGCAACAA TGTCTTCTAA TCGACCATTT ACTAACCAAT TCCAATCGAT TGATCCCACT
    TCAAACGACG CACCACACCA ACATCATGTA CATCAACACC AATACCAACA TCACCGAAAA
    ACAACAACGA CCATCATCAA TAACACTCTG AACGAAAACC ACACCACCGC CACAATAACA
    AACAACACTA CCACGACCCC GTCTTGTTGT ACCAAAACCA TCGCCACAGA TAGCCAAGTC
    TCGGTGTCGG TCTCGGCATC GGTGTCATCG GCCAACGCAT CCACGTCGTC GCGTCGCCAA
    AGACACAGTA TTGCCGGCCA GATGTCCTAT ATGAAAATGT TGGGTTTCGG TGGTTTTAGC
    AAAAAGATGG CCACCAGCGC AAATAGCCTT TTCAGCACCG CCGTCATCAG CGGCAGCTCG
    TCCGCTCCAA ATCTTCGCGA CATGATAACG GTCTCCTCTT CCGGATTTGG CGATGTACCA
    CCAATCCGCC CGCTGGAGAC ACTGCACAAC GCCCTTTCGC TGCGCAAGCT GGACAGTTTC
    TTGCAGGACA TGATCCTGGC GCAGATCTTT AAGACGCCGA CAGGGTCCCC GCCGCGGGGC
    TTCTCTAAGA ATACCTTGCC AGCGGTTTCC TCGATGACTC TAACCGCCTC AAATCAGACA
    GAGGTTGTCG AACATGAGCC GATATCAACG ACCGTAACGC CCACCTTTGA CCGGCGTGGC
    AGCGAGTCCG GATCCACAGC GGATGCCCAT CTGCGTCTTC TTTCGGAGAG CCAGTGCCCT
    AATCTGGACG ATAGCAACAC CGAGCTGCGA GAATCGCTGG CTGGAAAAAT GGAGCAATAT
    CCAACAGAGC CCAGCATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02273
    Clone ID Related Accession (Same CDS sequence) NM_176780.2
    Accession Version NM_176780.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 26, 2009 this sequence version replaced NM_176780.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GCAACAGAGA CAGCAGAAGA TACATCAATG
    GATATACAGC CAAAGATATG TATTGGAAAA TGTGCACCTG GGCTGCTTAC ACCAACAGTT
    ACTACTACCG ATATTTTAAA AGGTATACGA GACATCGCTC CTACAGCCAC CCAAACATCC
    CCACCAACAT TTACACCATC AACCACTACT ACAACTGGAG CAGCAGCAGC TTTGTTTGAT
    AATACAGCAA CAATGTCTTC TAATCGACCA TTTACTAACC AATTCCAATC GATTGATCCC
    ACTTCAAACG ACGCACCACA CCAACATCAT GTACATCAAC ACCAATACCA ACATCACCGA
    AAAACAACAA CGACCATCAT CAATAACACT CTGAACGAAA ACCACACCAC CGCCACAATA
    ACAAACAACA CTACCACGAC CCCGTCTTGT TGTACCAAAA CCATCGCCAC AGATAGCCAA
    GTCTCGGTGT CGGTCTCGGC ATCGGTGTCA TCGGCCAACG CATCCACGTC GTCGCGTCGC
    CAAAGACACA GTATTGCCGG CCAGATGTCC TATATGAAAA TGTTGGGTTT CGGTGGTTTT
    AGCAAAAAGA TGGCCACCAG CGCAAATAGC CTTTTCAGCA CCGCCGTCAT CAGCGGCAGC
    TCGTCCGCTC CAAATCTTCG CGACATGATA ACGGTCTCCT CTTCCGGATT TGGCGATGTA
    CCACCAATCC GCCCGCTGGA GACACTGCAC AACGCCCTTT CGCTGCGCAA GCTGGACAGT
    TTCTTGCAGG ACATGATCCT GGCGCAGATC TTTAAGACGC CGACAGGGTC CCCGCCGCGG
    GGCTTCTCTA AGAATACCTT GCCAGCGGTT TCCTCGATGA CTCTAACCGC CTCAAATCAG
    ACAGAGGTTG TCGAACATGA GCCGATATCA ACGACCGTAA CGCCCACCTT TGACCGGCGT
    GGCAGCGAGT CCGGATCCAC AGCGGATGCC CATCTGCGTC TTCTTTCGGA GAGCCAGTGC
    CCTAATCTGG ACGATAGCAA CACCGAGCTG CGAGAATCGC TGGCTGGAAA AATGGAGCAA
    TATCCAACAG AGCCCAGCAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788953.2
    CDS488..4831
    Translation

    Target ORF information:

    RefSeq Version NM_176780.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176780.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GCAACAGAGA CAGCAGAAGA TACATCAATG
    GATATACAGC CAAAGATATG TATTGGAAAA TGTGCACCTG GGCTGCTTAC ACCAACAGTT
    ACTACTACCG ATATTTTAAA AGGTATACGA GACATCGCTC CTACAGCCAC CCAAACATCC
    CCACCAACAT TTACACCATC AACCACTACT ACAACTGGAG CAGCAGCAGC TTTGTTTGAT
    AATACAGCAA CAATGTCTTC TAATCGACCA TTTACTAACC AATTCCAATC GATTGATCCC
    ACTTCAAACG ACGCACCACA CCAACATCAT GTACATCAAC ACCAATACCA ACATCACCGA
    AAAACAACAA CGACCATCAT CAATAACACT CTGAACGAAA ACCACACCAC CGCCACAATA
    ACAAACAACA CTACCACGAC CCCGTCTTGT TGTACCAAAA CCATCGCCAC AGATAGCCAA
    GTCTCGGTGT CGGTCTCGGC ATCGGTGTCA TCGGCCAACG CATCCACGTC GTCGCGTCGC
    CAAAGACACA GTATTGCCGG CCAGATGTCC TATATGAAAA TGTTGGGTTT CGGTGGTTTT
    AGCAAAAAGA TGGCCACCAG CGCAAATAGC CTTTTCAGCA CCGCCGTCAT CAGCGGCAGC
    TCGTCCGCTC CAAATCTTCG CGACATGATA ACGGTCTCCT CTTCCGGATT TGGCGATGTA
    CCACCAATCC GCCCGCTGGA GACACTGCAC AACGCCCTTT CGCTGCGCAA GCTGGACAGT
    TTCTTGCAGG ACATGATCCT GGCGCAGATC TTTAAGACGC CGACAGGGTC CCCGCCGCGG
    GGCTTCTCTA AGAATACCTT GCCAGCGGTT TCCTCGATGA CTCTAACCGC CTCAAATCAG
    ACAGAGGTTG TCGAACATGA GCCGATATCA ACGACCGTAA CGCCCACCTT TGACCGGCGT
    GGCAGCGAGT CCGGATCCAC AGCGGATGCC CATCTGCGTC TTCTTTCGGA GAGCCAGTGC
    CCTAATCTGG ACGATAGCAA CACCGAGCTG CGAGAATCGC TGGCTGGAAA AATGGAGCAA
    TATCCAACAG AGCCCAGCAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02275
    Clone ID Related Accession (Same CDS sequence) NM_176779.3
    Accession Version NM_176779.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_176779.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTGGA CTTGGTTCAA AGACTGGTGG CGTCTCAAGA AGCTCCGATC TCGTCACCAG 
    CGACATAAGA AAAAACTCCA AGCAACCGCG GCGTCCGCTG AAGCTGCGGT GACAGCAAGC
    GCATTTTTGG CCGGCTCTGG GTGCGCGGAA AGTGGTGGTT CCCAGGATCC TCAAACCAAT
    CGTCTCCACA GCTTGGACGC AGTGCCATCT GATGGAACTT TAGCCAGAAG ACGCCATGGT
    AGTCGTGATA GTCTACGCCG GAATCGCTTG CTGCAGCGCC AGCGACGGAG TCAAAGTGCC
    GCAGTTCGCA GTCATAGAAG CCACGACGAC GACGACGATG GGGAGTACGG ACCCTTCAAC
    GTCAGTGATT ACGAGGATTA CGGGCCAAAT CATGGCAGCG ACGAGGAGAG TGATGATTTC
    TGCTACTGTG AAGTCTGCAT GAATGGCGAC ACGGACTTCG GAGACAGCAA TGACGGGATG
    GACTCCGACA CGAGCACCTC TTCCAGCAAC AGCAAGCAGG TGGTTGTCGG CATCTGCGCG
    ATGGCCAAGA AGACACAGTC AAAGCCAATG AAGGAGATCC TGACACGTCT TGGAGAATTC
    GAGTTCATCA AACTGGTGAC GTTCGAGGAG AACGTTATCC TGCGAGAACC GGTCCAAAAT
    TGGCCCACCT GCGACTGCCT GGTATCGTTT CACTCGAAAG GCTTTCCCCT AGAGAAAGCC
    ATCGAGTATG CCCAGCTTAG GAATCCATTT GTGCTGAATA ATCTGCATAT GCAGTACGAT
    ATTCAGGACA GGAGAAGAGT GTACGCCATT CTGGAGAAGG AGGGTATTGA GATTCCGCGC
    TATGCCGTCC TCGATCGTGA TTCGCCGGAC CCTAAACACC ATGAGCTTAT TGAGTCCGAG
    GACCATGTGG AGGTCAATGG TATTACCTTT AACAAGCCGT TTGTTGAGAA GCCTGTGTCG
    GCGGAGGACC ACAACATATA CATATATTAC CCGACGTCGG CGGGGGGAGG AAGTCAGCGA
    CTTTTCCGAA AGATCGGCAG CCGGAGCAGC GTGTATTCTC CGGAATCAAG GGTGCGAAAG
    ACAGGATCAT TTATCTACGA GGACTTTATG CCCACCGATG TGTACTTTTC AGGCACGGAT
    GTCAAGGTGT ACACCGTGGG ACCAGACTAC GCCCATGCCG AAGCACGTAA AAGTCCCGCG
    TTGGATGGCA AAGTAGAGCG CGACAGCGAA GGAAAAGAGA TTCGCTATCC AGTGATCCTC
    AATCATTCCG AGAAGCTAAT CTCACGGAAG GTGTGCCTGG CCTTTAAGCA AACCGTCTGT
    GGATTTGATT TGTTGCGAGC CAACGGAAAG AGCTATGTTT GCGATGTTAA CGGCTTTAGC
    TTCGTGAAGA ACTCGAACAA GTACTATGAT GATTGTGCCA AAATACTGGG CAACATGATT
    CTCAGGGAGC TAACACCTAC CCTGCACATA CCCTGGTCAG TGCCCTTTCA ATTAGACGAT
    CCCCCCATTG TGCCCACTAC TTTCGGCAAA ATGATGGAGC TGCGCTGCGT GGTGGCCGTA
    ATTAGACATG GCGATCGCAC GCCCAAACAA AAAATGAAGG TTGAGGTGCG GCATCCCAAA
    TTTTTCGAGA TCTTCGAGAA GTACGACGGC TACAAGCTGG GCCACGTTAA GCTAAAGCGA
    CCCAAACAGC TTCAAGAGAT TTTGGACATC GCCCGCTTCC TGCTAAGCGA GATCCACACC
    AAAGCTCATG CCGAGATTGA GGAAAAGGAA AGCAAGCTTG AGCAGCTTAA GAACGTTCTG
    GAGATGTACG GTCACTTTTC GGGCATAAAC CGGAAGGTTC AAATGAAATA CCAACCAAAG
    GGACGTCCAC GTGGCTCCAG CTCCGATGAT ACCAATCTAG CAGCGGATCA ACCAGTGGAA
    CCTTCCCTGG TCCTCATCCT CAAGTGGGGC GGCGAACTGA CTCCAGCAGG CCGCATCCAG
    GCGGAGGAGT TGGGTCGCAT ATTTCGGTGC ATGTATCCAG GTGGCCAAGG AAGATCGGAT
    TACTCGGGCA CCCAAGGACT AGGTTTGCTA AGATTGCACT CGACGTTCCG GCATGACTTG
    AAGATCTACG CCTCAGATGA GGGTCGTGTC CAGATGACGG CTGCCGCTTT TGCAAAGGGT
    TTGCTGGCCC TGGAAGGAGA ACTTACTCCC ATTTTGGTCC AGATGGTAAA GAGTGCCAAT
    ACAAATGGAC TGCTGGACAA TGATTGCGAC TCCAGCAAGT ACCAAAACTT GGCTAAAGGT
    CGCCTACACG AGCTTATGCA AAACGATCGA GAGTTCTCTA AGGAGGATCG TGAGCTGATT
    AATCCTTGCA ATAGCAAATC GATTACCCAG GCTCTGGATT TTGTAAAAAA TCCTGTTGAC
    TGCTGTCACC ACGTACACCT GCTTATTCGC GAGCTTCTTC ACATTATCAG CATCAAAAAG
    GATGATCCAA AGACCAAGGA CGCCATCTTA TATCACGGCG AGACGTGGGA CCTGATGCGT
    TGTCGCTGGG AAAAAATTGA GAAGGATTTT AGCACCAAAT CGAAGCTGTT TGACATCTCA
    AAGATACCAG ATATATATGA TTGCATCAAA TACGATCTGC AACATAATCA GCACACGTTG
    CAATACGATC AGGCGGAGGA GCTATACATC TATGCGAAGA ATCTGGCTGA TATAGTCATA
    CCACAGGAGT ATGGACTGAC GCCACAGGAG AAACTGGCAA TTGGTCAAGG TATCTGCTCC
    CCATTACTGA GAAAGATCAA GGGCGATCTG CAGCGAAACA TCGACGAAGT GGAAGACGAG
    TTTATGAACC GTTTGAATCC ACATTATAGC CATGGTGTTG CTAGCCCTCA GAGGCATGTT
    CGTACAAGGC TCTATTTTAC CAGCGAATCG CATGTTCACT CGCTGCTCAC CGTTTTGCGT
    TATGGAGGAT TGCTCAATGT GGTCACAGAT GAGCAGTGGC GTCGGGCTAT GGACTATATT
    TCGATGGTAT CGGAGCTCAA CTATATGTCC CAGATCGTTA TCATGCTCTA CGAGGATCCG
    ACCAAAGATC CAACTTCTGA GGAACGCTTC CATGTCGAAC TGCACTTTAG TCCGGGTGTT
    AATTGCTGTG TGCAAAAGAA TCTACCACCA GGTCCGGGAT TTCGCCCGCA TTCGCACGGC
    GATAATGCTT GCAATGTAAG TTTGCAATCC TCGGACGAGT CAAATCCTGC CAGAATCGAG
    GAGGAGAATG ACTCGAATTC AGGAGAAGAA CGGGAGGGCA AAAAGCGAGG GACCTCAGGC
    CAAAGGAGTA CGGATCGCAG TGCGGAACGC ATCTCCCCTG CTTTCGGATT CAATCGATTA
    GAGCTCAGAT CCAAGCAGTT CAAATCGAAA CCTATCCCCA TCGGAGCCCA TCACACGGTC
    AGTGGCCATG AAGCAATGGA TCTAGCCAAG CGGCTAAACG AGGAGCTGGC ATCGCACCAA
    CAGCAGCAAA ACCAGCAGCT TCGACCCATC AGTCCGGATA TAAGGGCAGT AACTCCTGAC
    TGCGAACCAC GCTCCCGGAG TTTTGAGCAG CGTCCAACGT CCGGCGTATG TGCCAAAGAA
    CCGGATAGCC AAGTCTCGGT GTCGGTCTCG GCATCGGTGT CATCGGCCAA CGCATCCACG
    TCGTCGCGTC GCCAAAGACA CAGTATTGCC GGCCAGATGT CCTATATGAA AATGTTGGGT
    TTCGGTGGTT TTAGCAAAAA GATGGCCACC AGCGCAAATA GCCTTTTCAG CACCGCCGTC
    ATCAGCGGCA GCTCGTCCGC TCCAAATCTT CGCGACATGA TAACGGTCTC CTCTTCCGGA
    TTTGGCGATG TACCACCAAT CCGCCCGCTG GAGACACTGC ACAACGCCCT TTCGCTGCGC
    AAGCTGGACA GTTTCTTGCA GGACATGATC CTGGCGCAGA TCTTTAAGAC GCCGACAGGG
    TCCCCGCCGC GGGGCTTCTC TAAGAATACC TTGCCAGCGG TTTCCTCGAT GACTCTAACC
    GCCTCAAATC AGACAGAGGT TGTCGAACAT GAGCCGATAT CAACGACCGT AACGCCCACC
    TTTGACCGGC GTGGCAGCGA GTCCGGATCC ACAGCGGATG CCCATCTGCG TCTTCTTTCG
    GAGAGCCAGT GCCCTAATCT GGACGATAGC AACACCGAGC TGCGAGAATC GCTGGCTGGA
    AAAATGGAGC GTAAGTGCCG AAAAAAGTCA GTGGTCGAGT GGCAGTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788952.2
    CDS384..4634
    Translation

    Target ORF information:

    RefSeq Version NM_176779.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176779.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTGGA CTTGGTTCAA AGACTGGTGG CGTCTCAAGA AGCTCCGATC TCGTCACCAG 
    CGACATAAGA AAAAACTCCA AGCAACCGCG GCGTCCGCTG AAGCTGCGGT GACAGCAAGC
    GCATTTTTGG CCGGCTCTGG GTGCGCGGAA AGTGGTGGTT CCCAGGATCC TCAAACCAAT
    CGTCTCCACA GCTTGGACGC AGTGCCATCT GATGGAACTT TAGCCAGAAG ACGCCATGGT
    AGTCGTGATA GTCTACGCCG GAATCGCTTG CTGCAGCGCC AGCGACGGAG TCAAAGTGCC
    GCAGTTCGCA GTCATAGAAG CCACGACGAC GACGACGATG GGGAGTACGG ACCCTTCAAC
    GTCAGTGATT ACGAGGATTA CGGGCCAAAT CATGGCAGCG ACGAGGAGAG TGATGATTTC
    TGCTACTGTG AAGTCTGCAT GAATGGCGAC ACGGACTTCG GAGACAGCAA TGACGGGATG
    GACTCCGACA CGAGCACCTC TTCCAGCAAC AGCAAGCAGG TGGTTGTCGG CATCTGCGCG
    ATGGCCAAGA AGACACAGTC AAAGCCAATG AAGGAGATCC TGACACGTCT TGGAGAATTC
    GAGTTCATCA AACTGGTGAC GTTCGAGGAG AACGTTATCC TGCGAGAACC GGTCCAAAAT
    TGGCCCACCT GCGACTGCCT GGTATCGTTT CACTCGAAAG GCTTTCCCCT AGAGAAAGCC
    ATCGAGTATG CCCAGCTTAG GAATCCATTT GTGCTGAATA ATCTGCATAT GCAGTACGAT
    ATTCAGGACA GGAGAAGAGT GTACGCCATT CTGGAGAAGG AGGGTATTGA GATTCCGCGC
    TATGCCGTCC TCGATCGTGA TTCGCCGGAC CCTAAACACC ATGAGCTTAT TGAGTCCGAG
    GACCATGTGG AGGTCAATGG TATTACCTTT AACAAGCCGT TTGTTGAGAA GCCTGTGTCG
    GCGGAGGACC ACAACATATA CATATATTAC CCGACGTCGG CGGGGGGAGG AAGTCAGCGA
    CTTTTCCGAA AGATCGGCAG CCGGAGCAGC GTGTATTCTC CGGAATCAAG GGTGCGAAAG
    ACAGGATCAT TTATCTACGA GGACTTTATG CCCACCGATG TGTACTTTTC AGGCACGGAT
    GTCAAGGTGT ACACCGTGGG ACCAGACTAC GCCCATGCCG AAGCACGTAA AAGTCCCGCG
    TTGGATGGCA AAGTAGAGCG CGACAGCGAA GGAAAAGAGA TTCGCTATCC AGTGATCCTC
    AATCATTCCG AGAAGCTAAT CTCACGGAAG GTGTGCCTGG CCTTTAAGCA AACCGTCTGT
    GGATTTGATT TGTTGCGAGC CAACGGAAAG AGCTATGTTT GCGATGTTAA CGGCTTTAGC
    TTCGTGAAGA ACTCGAACAA GTACTATGAT GATTGTGCCA AAATACTGGG CAACATGATT
    CTCAGGGAGC TAACACCTAC CCTGCACATA CCCTGGTCAG TGCCCTTTCA ATTAGACGAT
    CCCCCCATTG TGCCCACTAC TTTCGGCAAA ATGATGGAGC TGCGCTGCGT GGTGGCCGTA
    ATTAGACATG GCGATCGCAC GCCCAAACAA AAAATGAAGG TTGAGGTGCG GCATCCCAAA
    TTTTTCGAGA TCTTCGAGAA GTACGACGGC TACAAGCTGG GCCACGTTAA GCTAAAGCGA
    CCCAAACAGC TTCAAGAGAT TTTGGACATC GCCCGCTTCC TGCTAAGCGA GATCCACACC
    AAAGCTCATG CCGAGATTGA GGAAAAGGAA AGCAAGCTTG AGCAGCTTAA GAACGTTCTG
    GAGATGTACG GTCACTTTTC GGGCATAAAC CGGAAGGTTC AAATGAAATA CCAACCAAAG
    GGACGTCCAC GTGGCTCCAG CTCCGATGAT ACCAATCTAG CAGCGGATCA ACCAGTGGAA
    CCTTCCCTGG TCCTCATCCT CAAGTGGGGC GGCGAACTGA CTCCAGCAGG CCGCATCCAG
    GCGGAGGAGT TGGGTCGCAT ATTTCGGTGC ATGTATCCAG GTGGCCAAGG AAGATCGGAT
    TACTCGGGCA CCCAAGGACT AGGTTTGCTA AGATTGCACT CGACGTTCCG GCATGACTTG
    AAGATCTACG CCTCAGATGA GGGTCGTGTC CAGATGACGG CTGCCGCTTT TGCAAAGGGT
    TTGCTGGCCC TGGAAGGAGA ACTTACTCCC ATTTTGGTCC AGATGGTAAA GAGTGCCAAT
    ACAAATGGAC TGCTGGACAA TGATTGCGAC TCCAGCAAGT ACCAAAACTT GGCTAAAGGT
    CGCCTACACG AGCTTATGCA AAACGATCGA GAGTTCTCTA AGGAGGATCG TGAGCTGATT
    AATCCTTGCA ATAGCAAATC GATTACCCAG GCTCTGGATT TTGTAAAAAA TCCTGTTGAC
    TGCTGTCACC ACGTACACCT GCTTATTCGC GAGCTTCTTC ACATTATCAG CATCAAAAAG
    GATGATCCAA AGACCAAGGA CGCCATCTTA TATCACGGCG AGACGTGGGA CCTGATGCGT
    TGTCGCTGGG AAAAAATTGA GAAGGATTTT AGCACCAAAT CGAAGCTGTT TGACATCTCA
    AAGATACCAG ATATATATGA TTGCATCAAA TACGATCTGC AACATAATCA GCACACGTTG
    CAATACGATC AGGCGGAGGA GCTATACATC TATGCGAAGA ATCTGGCTGA TATAGTCATA
    CCACAGGAGT ATGGACTGAC GCCACAGGAG AAACTGGCAA TTGGTCAAGG TATCTGCTCC
    CCATTACTGA GAAAGATCAA GGGCGATCTG CAGCGAAACA TCGACGAAGT GGAAGACGAG
    TTTATGAACC GTTTGAATCC ACATTATAGC CATGGTGTTG CTAGCCCTCA GAGGCATGTT
    CGTACAAGGC TCTATTTTAC CAGCGAATCG CATGTTCACT CGCTGCTCAC CGTTTTGCGT
    TATGGAGGAT TGCTCAATGT GGTCACAGAT GAGCAGTGGC GTCGGGCTAT GGACTATATT
    TCGATGGTAT CGGAGCTCAA CTATATGTCC CAGATCGTTA TCATGCTCTA CGAGGATCCG
    ACCAAAGATC CAACTTCTGA GGAACGCTTC CATGTCGAAC TGCACTTTAG TCCGGGTGTT
    AATTGCTGTG TGCAAAAGAA TCTACCACCA GGTCCGGGAT TTCGCCCGCA TTCGCACGGC
    GATAATGCTT GCAATGTAAG TTTGCAATCC TCGGACGAGT CAAATCCTGC CAGAATCGAG
    GAGGAGAATG ACTCGAATTC AGGAGAAGAA CGGGAGGGCA AAAAGCGAGG GACCTCAGGC
    CAAAGGAGTA CGGATCGCAG TGCGGAACGC ATCTCCCCTG CTTTCGGATT CAATCGATTA
    GAGCTCAGAT CCAAGCAGTT CAAATCGAAA CCTATCCCCA TCGGAGCCCA TCACACGGTC
    AGTGGCCATG AAGCAATGGA TCTAGCCAAG CGGCTAAACG AGGAGCTGGC ATCGCACCAA
    CAGCAGCAAA ACCAGCAGCT TCGACCCATC AGTCCGGATA TAAGGGCAGT AACTCCTGAC
    TGCGAACCAC GCTCCCGGAG TTTTGAGCAG CGTCCAACGT CCGGCGTATG TGCCAAAGAA
    CCGGATAGCC AAGTCTCGGT GTCGGTCTCG GCATCGGTGT CATCGGCCAA CGCATCCACG
    TCGTCGCGTC GCCAAAGACA CAGTATTGCC GGCCAGATGT CCTATATGAA AATGTTGGGT
    TTCGGTGGTT TTAGCAAAAA GATGGCCACC AGCGCAAATA GCCTTTTCAG CACCGCCGTC
    ATCAGCGGCA GCTCGTCCGC TCCAAATCTT CGCGACATGA TAACGGTCTC CTCTTCCGGA
    TTTGGCGATG TACCACCAAT CCGCCCGCTG GAGACACTGC ACAACGCCCT TTCGCTGCGC
    AAGCTGGACA GTTTCTTGCA GGACATGATC CTGGCGCAGA TCTTTAAGAC GCCGACAGGG
    TCCCCGCCGC GGGGCTTCTC TAAGAATACC TTGCCAGCGG TTTCCTCGAT GACTCTAACC
    GCCTCAAATC AGACAGAGGT TGTCGAACAT GAGCCGATAT CAACGACCGT AACGCCCACC
    TTTGACCGGC GTGGCAGCGA GTCCGGATCC ACAGCGGATG CCCATCTGCG TCTTCTTTCG
    GAGAGCCAGT GCCCTAATCT GGACGATAGC AACACCGAGC TGCGAGAATC GCTGGCTGGA
    AAAATGGAGC GTAAGTGCCG AAAAAAGTCA GTGGTCGAGT GGCAGTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21569
    Clone ID Related Accession (Same CDS sequence) NM_001298606.1
    Accession Version NM_001298606.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5103bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGT GTACTTTTCA GGCACGGATG TCAAGGTGTA CACCGTGGGA
    CCAGACTACG CCCATGCCGA AGCACGTAAA AGTCCCGCGT TGGATGGCAA AGTAGAGCGC
    GACAGCGAAG GAAAAGAGAT TCGCTATCCA GTGATCCTCA ATCATTCCGA GAAGCTAATC
    TCACGGAAGG TGTGCCTGGC CTTTAAGCAA ACCGTCTGTG GATTTGATTT GTTGCGAGCC
    AACGGAAAGA GCTATGTTTG CGATGTTAAC GGCTTTAGCT TCGTGAAGAA CTCGAACAAG
    TACTATGATG ATTGTGCCAA AATACTGGGC AACATGATTC TCAGGGAGCT AACACCTACC
    CTGCACATAC CCTGGTCAGT GCCCTTTCAA TTAGACGATC CCCCCATTGT GCCCACTACT
    TTCGGCAAAA TGATGGAGCT GCGCTGCGTG GTGGCCGTAA TTAGACATGG CGATCGCACG
    CCCAAACAAA AAATGAAGGT TGAGGTGCGG CATCCCAAAT TTTTCGAGAT CTTCGAGAAG
    TACGACGGCT ACAAGCTGGG CCACGTTAAG CTAAAGCGAC CCAAACAGCT TCAAGAGATT
    TTGGACATCG CCCGCTTCCT GCTAAGCGAG ATCCACACCA AAGCTCATGC CGAGATTGAG
    GAAAAGGAAA GCAAGCTTGA GCAGCTTAAG AACGTTCTGG AGATGTACGG TCACTTTTCG
    GGCATAAACC GGAAGGTTCA AATGAAATAC CAACCAAAGG GACGTCCACG TGGCTCCAGC
    TCCGATGATA CCAATCTAGC AGCGGATCAA CCAGTGGAAC CTTCCCTGGT CCTCATCCTC
    AAGTGGGGCG GCGAACTGAC TCCAGCAGGC CGCATCCAGG CGGAGGAGTT GGGTCGCATA
    TTTCGGTGCA TGTATCCAGG TGGCCAAGGA AGATCGGATT ACTCGGGCAC CCAAGGACTA
    GGTTTGCTAA GATTGCACTC GACGTTCCGG CATGACTTGA AGATCTACGC CTCAGATGAG
    GGTCGTGTCC AGATGACGGC TGCCGCTTTT GCAAAGGGTT TGCTGGCCCT GGAAGGAGAA
    CTTACTCCCA TTTTGGTCCA GATGGTAAAG AGTGCCAATA CAAATGGACT GCTGGACAAT
    GATTGCGACT CCAGCAAGTA CCAAAACTTG GCTAAAGGTC GCCTACACGA GCTTATGCAA
    AACGATCGAG AGTTCTCTAA GGAGGATCGT GAGCTGATTA ATCCTTGCAA TAGCAAATCG
    ATTACCCAGG CTCTGGATTT TGTAAAAAAT CCTGTTGACT GCTGTCACCA CGTACACCTG
    CTTATTCGCG AGCTTCTTCA CATTATCAGC ATCAAAAAGG ATGATCCAAA GACCAAGGAC
    GCCATCTTAT ATCACGGCGA GACGTGGGAC CTGATGCGTT GTCGCTGGGA AAAAATTGAG
    AAGGATTTTA GCACCAAATC GAAGCTGTTT GACATCTCAA AGATACCAGA TATATATGAT
    TGCATCAAAT ACGATCTGCA ACATAATCAG CACACGTTGC AATACGATCA GGCGGAGGAG
    CTATACATCT ATGCGAAGAA TCTGGCTGAT ATAGTCATAC CACAGGAGTA TGGACTGACG
    CCACAGGAGA AACTGGCAAT TGGTCAAGGT ATCTGCTCCC CATTACTGAG AAAGATCAAG
    GGCGATCTGC AGCGAAACAT CGACGAAGTG GAAGACGAGT TTATGAACCG TTTGAATCCA
    CATTATAGCC ATGGTGTTGC TAGCCCTCAG AGGCATGTTC GTACAAGGCT CTATTTTACC
    AGCGAATCGC ATGTTCACTC GCTGCTCACC GTTTTGCGTT ATGGAGGATT GCTCAATGTG
    GTCACAGATG AGCAGTGGCG TCGGGCTATG GACTATATTT CGATGGTATC GGAGCTCAAC
    TATATGTCCC AGATCGTTAT CATGCTCTAC GAGGATCCGA CCAAAGATCC AACTTCTGAG
    GAACGCTTCC ATGTCGAACT GCACTTTAGT CCGGGTGTTA ATTGCTGTGT GCAAAAGAAT
    CTACCACCAG GTCCGGGATT TCGCCCGCAT TCGCACGGCG ATAATGCTTG CAATGTAAGT
    TTGCAATCCT CGGACGAGTC AAATCCTGCC AGAATCGAGG AGGAGAATGA CTCGAATTCA
    GGAGAAGAAC GGGAGGGCAA AAAGCGAGGG ACCTCAGGCC AAAGGAGTAC GGATCGCAGT
    GCGGAACGCA TCTCCCCTGC TTTCGGATTC AATCGATTAG AGCTCAGATC CAAGCAGTTC
    AAATCGAAAC CTATCCCCAT CGGAGCCCAT CACACGGTCA GTGGCCATGA AGCAATGGAT
    CTAGCCAAGC GGCTAAACGA GGAGCTGGCA TCGCACCAAC AGCAGCAAAA CCAGCAGCTT
    CGACCCATCA GTCCGGATAT AAGGGCAGTA ACTCCTGACT GCGAACCACG CTCCCGGAGT
    TTTGAGCAGC GTCCAACGTC CGGCGTATGT GCCAAAGAAC CGGATAGCCA AGTCTCGGTG
    TCGGTCTCGG CATCGGTGTC ATCGGCCAAC GCATCCACGT CGTCGCGTCG CCAAAGACAC
    AGTATTGCCG GCCAGATGTC CTATATGAAA ATGTTGGGTT TCGGTGGTTT TAGCAAAAAG
    ATGGCCACCA GCGCAAATAG CCTTTTCAGC ACCGCCGTCA TCAGCGGCAG CTCGTCCGCT
    CCAAATCTTC GCGACATGAT AACGGTCTCC TCTTCCGGAT TTGGCGATGT ACCACCAATC
    CGCCCGCTGG AGACACTGCA CAACGCCCTT TCGCTGCGCA AGCTGGACAG TTTCTTGCAG
    GACATGATCC TGGCGCAGAT CTTTAAGACG CCGACAGGGT CCCCGCCGCG GGGCTTCTCT
    AAGAATACCT TGCCAGCGGT TTCCTCGATG ACTCTAACCG CCTCAAATCA GACAGAGGTT
    GTCGAACATG AGCCGATATC AACGACCGTA ACGCCCACCT TTGACCGGCG TGGCAGCGAG
    TCCGGATCCA CAGCGGATGC CCATCTGCGT CTTCTTTCGG AGAGCCAGTG CCCTAATCTG
    GACGATAGCA ACACCGAGCT GCGAGAATCG CTGGCTGGAA AAATGGAGCT TTGGCCACAG
    GACATTGTGA AGGCAGCCGA GGATGAGGAA TTAAACCTGT CAGAATTGGA AACAAAACCA
    AGCTTGGACC TTACCATGGA GATTATGGAG CGCGGGGTCG CGGGTATCGC CTCCATCCAG
    CCAATGAATC GGGACAGCGA TGAAACGATG GGCGGTGGAG TCTTTCTGAG TGTTTGTGAG
    GAACAGGGCA GCGGAAGCAC CTGCCTTACA CCAGTTAGTT TTGGGATGGA TCTCGACCTG
    AGCATGGTGG CTAACAAGGG CTCCATTACG CTGTCAATGG AAGGGTTCGA TGATGATGAA
    GATGCCACTT TGTCGGCGGC CACAACACCT TCCCTTTTGG CGGACTGTGA GCCGCGCCTG
    GAGAGTTGCT ATTGTTGCCC CAGCCATGCA GAGGCCCCGC CTGAGGTTCC CAGCGATGAT
    CCACGGTTTG GCTTTGCGCT GCCCGTTCGA GTCACACAAG CTTCACCGGA GCATGCGCGA
    CCTGTGCGCC GTGCTCATGA TCCCGTCTCG CCAAGGATCC AGAAACAGAT CAGCTTGTTC
    GAGGGAAACG CGGCTATGGC AACGGGTCAG GAAAAGACTG AGTCCAGCGG GTCGGTTGGG
    GGCGGTGCAA TTCTGCACGC CTCCATTAAC CTGCCGGCGG CGGGACCGCA TCATTTGCGC
    CAAGATGCTC GCCTGCGCAA GTTTGAGAAC CTCACGCAGT CCACATCAAA CTCGAACTTT
    CCCTTCGAAA GCAACACGCT GAAGCGGGTG CCCATGCAGA CCACCAAAGA CTACGACAAC
    GTGAGCCACA CACAGAGCTG CATAAATCTC AAAAGTGGCA GCAGTGGAGT CCTCGGGGGA
    TCGCCGCAGC GTCAGAGAGG ATCCGATGGA GGAGGCGTAG GTGCAAGTGG AGTCCCGGCA
    GAGAGCCGGG AGCCGACGCG GGTGCATTAT GGACGGATGA ACAGTACATG CTGCTCTGCC
    TCGGCATCGC CGTCCCCCTC CCCGGGGGCG CTGATTGTCA AGGAGCGGTT CATCGAACCG
    CCCAAGCGCG GTGTCGTGCG CGGATACCAT GGCAAAACGC AGTCCATGGA CGCCGACTTC
    CTGTTCAACG AGTTTCTACT GCTGCCGGCC ATGGCGCCCG CCAAGATATC CTTTGATAGT
    TCCGATATAG ACCAGGCCAG CGATGACGAC TCCCCGAGCA GCTCGAAACA AAGGCACGCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285535.1
    CDS488..5590
    Translation

    Target ORF information:

    RefSeq Version NM_001298606.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant O, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298606.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGT GTACTTTTCA GGCACGGATG TCAAGGTGTA CACCGTGGGA
    CCAGACTACG CCCATGCCGA AGCACGTAAA AGTCCCGCGT TGGATGGCAA AGTAGAGCGC
    GACAGCGAAG GAAAAGAGAT TCGCTATCCA GTGATCCTCA ATCATTCCGA GAAGCTAATC
    TCACGGAAGG TGTGCCTGGC CTTTAAGCAA ACCGTCTGTG GATTTGATTT GTTGCGAGCC
    AACGGAAAGA GCTATGTTTG CGATGTTAAC GGCTTTAGCT TCGTGAAGAA CTCGAACAAG
    TACTATGATG ATTGTGCCAA AATACTGGGC AACATGATTC TCAGGGAGCT AACACCTACC
    CTGCACATAC CCTGGTCAGT GCCCTTTCAA TTAGACGATC CCCCCATTGT GCCCACTACT
    TTCGGCAAAA TGATGGAGCT GCGCTGCGTG GTGGCCGTAA TTAGACATGG CGATCGCACG
    CCCAAACAAA AAATGAAGGT TGAGGTGCGG CATCCCAAAT TTTTCGAGAT CTTCGAGAAG
    TACGACGGCT ACAAGCTGGG CCACGTTAAG CTAAAGCGAC CCAAACAGCT TCAAGAGATT
    TTGGACATCG CCCGCTTCCT GCTAAGCGAG ATCCACACCA AAGCTCATGC CGAGATTGAG
    GAAAAGGAAA GCAAGCTTGA GCAGCTTAAG AACGTTCTGG AGATGTACGG TCACTTTTCG
    GGCATAAACC GGAAGGTTCA AATGAAATAC CAACCAAAGG GACGTCCACG TGGCTCCAGC
    TCCGATGATA CCAATCTAGC AGCGGATCAA CCAGTGGAAC CTTCCCTGGT CCTCATCCTC
    AAGTGGGGCG GCGAACTGAC TCCAGCAGGC CGCATCCAGG CGGAGGAGTT GGGTCGCATA
    TTTCGGTGCA TGTATCCAGG TGGCCAAGGA AGATCGGATT ACTCGGGCAC CCAAGGACTA
    GGTTTGCTAA GATTGCACTC GACGTTCCGG CATGACTTGA AGATCTACGC CTCAGATGAG
    GGTCGTGTCC AGATGACGGC TGCCGCTTTT GCAAAGGGTT TGCTGGCCCT GGAAGGAGAA
    CTTACTCCCA TTTTGGTCCA GATGGTAAAG AGTGCCAATA CAAATGGACT GCTGGACAAT
    GATTGCGACT CCAGCAAGTA CCAAAACTTG GCTAAAGGTC GCCTACACGA GCTTATGCAA
    AACGATCGAG AGTTCTCTAA GGAGGATCGT GAGCTGATTA ATCCTTGCAA TAGCAAATCG
    ATTACCCAGG CTCTGGATTT TGTAAAAAAT CCTGTTGACT GCTGTCACCA CGTACACCTG
    CTTATTCGCG AGCTTCTTCA CATTATCAGC ATCAAAAAGG ATGATCCAAA GACCAAGGAC
    GCCATCTTAT ATCACGGCGA GACGTGGGAC CTGATGCGTT GTCGCTGGGA AAAAATTGAG
    AAGGATTTTA GCACCAAATC GAAGCTGTTT GACATCTCAA AGATACCAGA TATATATGAT
    TGCATCAAAT ACGATCTGCA ACATAATCAG CACACGTTGC AATACGATCA GGCGGAGGAG
    CTATACATCT ATGCGAAGAA TCTGGCTGAT ATAGTCATAC CACAGGAGTA TGGACTGACG
    CCACAGGAGA AACTGGCAAT TGGTCAAGGT ATCTGCTCCC CATTACTGAG AAAGATCAAG
    GGCGATCTGC AGCGAAACAT CGACGAAGTG GAAGACGAGT TTATGAACCG TTTGAATCCA
    CATTATAGCC ATGGTGTTGC TAGCCCTCAG AGGCATGTTC GTACAAGGCT CTATTTTACC
    AGCGAATCGC ATGTTCACTC GCTGCTCACC GTTTTGCGTT ATGGAGGATT GCTCAATGTG
    GTCACAGATG AGCAGTGGCG TCGGGCTATG GACTATATTT CGATGGTATC GGAGCTCAAC
    TATATGTCCC AGATCGTTAT CATGCTCTAC GAGGATCCGA CCAAAGATCC AACTTCTGAG
    GAACGCTTCC ATGTCGAACT GCACTTTAGT CCGGGTGTTA ATTGCTGTGT GCAAAAGAAT
    CTACCACCAG GTCCGGGATT TCGCCCGCAT TCGCACGGCG ATAATGCTTG CAATGTAAGT
    TTGCAATCCT CGGACGAGTC AAATCCTGCC AGAATCGAGG AGGAGAATGA CTCGAATTCA
    GGAGAAGAAC GGGAGGGCAA AAAGCGAGGG ACCTCAGGCC AAAGGAGTAC GGATCGCAGT
    GCGGAACGCA TCTCCCCTGC TTTCGGATTC AATCGATTAG AGCTCAGATC CAAGCAGTTC
    AAATCGAAAC CTATCCCCAT CGGAGCCCAT CACACGGTCA GTGGCCATGA AGCAATGGAT
    CTAGCCAAGC GGCTAAACGA GGAGCTGGCA TCGCACCAAC AGCAGCAAAA CCAGCAGCTT
    CGACCCATCA GTCCGGATAT AAGGGCAGTA ACTCCTGACT GCGAACCACG CTCCCGGAGT
    TTTGAGCAGC GTCCAACGTC CGGCGTATGT GCCAAAGAAC CGGATAGCCA AGTCTCGGTG
    TCGGTCTCGG CATCGGTGTC ATCGGCCAAC GCATCCACGT CGTCGCGTCG CCAAAGACAC
    AGTATTGCCG GCCAGATGTC CTATATGAAA ATGTTGGGTT TCGGTGGTTT TAGCAAAAAG
    ATGGCCACCA GCGCAAATAG CCTTTTCAGC ACCGCCGTCA TCAGCGGCAG CTCGTCCGCT
    CCAAATCTTC GCGACATGAT AACGGTCTCC TCTTCCGGAT TTGGCGATGT ACCACCAATC
    CGCCCGCTGG AGACACTGCA CAACGCCCTT TCGCTGCGCA AGCTGGACAG TTTCTTGCAG
    GACATGATCC TGGCGCAGAT CTTTAAGACG CCGACAGGGT CCCCGCCGCG GGGCTTCTCT
    AAGAATACCT TGCCAGCGGT TTCCTCGATG ACTCTAACCG CCTCAAATCA GACAGAGGTT
    GTCGAACATG AGCCGATATC AACGACCGTA ACGCCCACCT TTGACCGGCG TGGCAGCGAG
    TCCGGATCCA CAGCGGATGC CCATCTGCGT CTTCTTTCGG AGAGCCAGTG CCCTAATCTG
    GACGATAGCA ACACCGAGCT GCGAGAATCG CTGGCTGGAA AAATGGAGCT TTGGCCACAG
    GACATTGTGA AGGCAGCCGA GGATGAGGAA TTAAACCTGT CAGAATTGGA AACAAAACCA
    AGCTTGGACC TTACCATGGA GATTATGGAG CGCGGGGTCG CGGGTATCGC CTCCATCCAG
    CCAATGAATC GGGACAGCGA TGAAACGATG GGCGGTGGAG TCTTTCTGAG TGTTTGTGAG
    GAACAGGGCA GCGGAAGCAC CTGCCTTACA CCAGTTAGTT TTGGGATGGA TCTCGACCTG
    AGCATGGTGG CTAACAAGGG CTCCATTACG CTGTCAATGG AAGGGTTCGA TGATGATGAA
    GATGCCACTT TGTCGGCGGC CACAACACCT TCCCTTTTGG CGGACTGTGA GCCGCGCCTG
    GAGAGTTGCT ATTGTTGCCC CAGCCATGCA GAGGCCCCGC CTGAGGTTCC CAGCGATGAT
    CCACGGTTTG GCTTTGCGCT GCCCGTTCGA GTCACACAAG CTTCACCGGA GCATGCGCGA
    CCTGTGCGCC GTGCTCATGA TCCCGTCTCG CCAAGGATCC AGAAACAGAT CAGCTTGTTC
    GAGGGAAACG CGGCTATGGC AACGGGTCAG GAAAAGACTG AGTCCAGCGG GTCGGTTGGG
    GGCGGTGCAA TTCTGCACGC CTCCATTAAC CTGCCGGCGG CGGGACCGCA TCATTTGCGC
    CAAGATGCTC GCCTGCGCAA GTTTGAGAAC CTCACGCAGT CCACATCAAA CTCGAACTTT
    CCCTTCGAAA GCAACACGCT GAAGCGGGTG CCCATGCAGA CCACCAAAGA CTACGACAAC
    GTGAGCCACA CACAGAGCTG CATAAATCTC AAAAGTGGCA GCAGTGGAGT CCTCGGGGGA
    TCGCCGCAGC GTCAGAGAGG ATCCGATGGA GGAGGCGTAG GTGCAAGTGG AGTCCCGGCA
    GAGAGCCGGG AGCCGACGCG GGTGCATTAT GGACGGATGA ACAGTACATG CTGCTCTGCC
    TCGGCATCGC CGTCCCCCTC CCCGGGGGCG CTGATTGTCA AGGAGCGGTT CATCGAACCG
    CCCAAGCGCG GTGTCGTGCG CGGATACCAT GGCAAAACGC AGTCCATGGA CGCCGACTTC
    CTGTTCAACG AGTTTCTACT GCTGCCGGCC ATGGCGCCCG CCAAGATATC CTTTGATAGT
    TCCGATATAG ACCAGGCCAG CGATGACGAC TCCCCGAGCA GCTCGAAACA AAGGCACGCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21570
    Clone ID Related Accession (Same CDS sequence) NM_001298607.1
    Accession Version NM_001298607.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GTAGTTTCTT CTCCTGATTT CGGGGATAAT
    TCTCGGACTC GTTCTTCTGA ATTCGGGGAG ACTTGTCATG CTCGAGTGGG CACTGCTAGT
    AACTCAGATG GGGGCGGGTC TCATCCGAGA GCGGATCATC GCACTGCCTT TCAGATTCGT
    GTTACAAATA GTTTGTCCTT CTTTAAAATT GACTCTTCGA CAAACGAGCT GCCTTTGTCG
    GACATTGATT TTTCGCTGCA CCCACCCACT CCGCAATGCG GTCCCTTGTC GCACAAGCGC
    TTCCACATCT TGACTATGCG GCGGATGGAG AGTTGCGACG ATGGTGCAGA ACTCGAACAG
    GTTCGTCATT TGCCACAGAT CTCGCCGATG GCCACCAATG AGCGACCCTT AAGTTCCTGC
    AATTGCAGTA GCTCAGCGGC CCAAGCGCAT TCGCACTCAA AAAGTCTGAT GGACTTGGCA
    CAAGCGGTGG TTATGACTTC ACCGCAAGAA ACTGGACCGA ATTCGGAGCA AGGTTGTGAC
    CCAACAACGG CGGCTGATCT CTGTCGGGAC AAGGACAAGG CCCTGGCTTC TGGCACATCC
    TCTGCACACA GCAAAGCCAC TAGTAACTCC TGTGGTCAGC TCTCCATGGC AGGTTCTGCT
    CCTGTGGTGA CAGAAAACCC ATTTCGATTC ACCGTTTCGT CATTGGGCTC TGCAGCTACC
    AATACCGCCT GTTTTGTAGG AAGCTTCGAG CCCATTGAAG AGCAGATCAC GTCCATTGTG
    GAGGTAGATT CAAAGCCCCT GCAGCCAGAG CCTCAAGTAG TATATAATCT GCCCACTGTG
    CTCATTACGG GAACAGCAAG TAGCTCAGAA TTGTCGACCA AACTAAATAG CAATATACTA
    CCGACTGTTA CAAACGCTTT TTCTGCAATA GATACCGAAA TAAATGACGA AATAGGTATA
    TCGAAGGAAG TAGGAATAGG GACAATAAGA ATTACAAACA CACATACACC CTGCAATAAG
    GCAACAGTTA CTCGACCAGA TACAAAAATA GCTATAACAA CTGATCCACA AACGGTTACA
    GCAACAGAGA CAGCAGAAGA TACATCAATG GATATACAGC CAAAGATATG TATTGGAAAA
    TGTGCACCTG GGCTGCTTAC ACCAACAGTT ACTACTACCG ATATTTTAAA AGATAGCCAA
    GTCTCGGTGT CGGTCTCGGC ATCGGTGTCA TCGGCCAACG CATCCACGTC GTCGCGTCGC
    CAAAGACACA GTATTGCCGG CCAGATGTCC TATATGAAAA TGTTGGGTTT CGGTGGTTTT
    AGCAAAAAGA TGGCCACCAG CGCAAATAGC CTTTTCAGCA CCGCCGTCAT CAGCGGCAGC
    TCGTCCGCTC CAAATCTTCG CGACATGATA ACGGTCTCCT CTTCCGGATT TGGCGATGTA
    CCACCAATCC GCCCGCTGGA GACACTGCAC AACGCCCTTT CGCTGCGCAA GCTGGACAGT
    TTCTTGCAGG ACATGATCCT GGCGCAGATC TTTAAGACGC CGACAGGGTC CCCGCCGCGG
    GGCTTCTCTA AGAATACCTT GCCAGCGGTT TCCTCGATGA CTCTAACCGC CTCAAATCAG
    ACAGAGGTTG TCGAACATGA GCCGATATCA ACGACCGTAA CGCCCACCTT TGACCGGCGT
    GGCAGCGAGT CCGGATCCAC AGCGGATGCC CATCTGCGTC TTCTTTCGGA GAGCCAGTGC
    CCTAATCTGG ACGATAGCAA CACCGAGCTG CGAGAATCGC TGGCTGGAAA AATGGAGCAA
    TATCCAACAG AGCCCAGCAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285536.1
    CDS488..5551
    Translation

    Target ORF information:

    RefSeq Version NM_001298607.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant P, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298607.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCTACA CCGAGTTGGA ATCAGGGTAC CAGGATATAT CCCAGCAGCA TCAGCAGCAA 
    AATCCGCATC AAAGTCAGCC GCAGCAGCGG GTCGGTTTCT ACTTGGGATT ACAGGATGGC
    AACGGCGACA CGGACTTCGG AGACAGCAAT GACGGGATGG ACTCCGACAC GAGCACCTCT
    TCCAGCAACA GCAAGCAGGT GGTTGTCGGC ATCTGCGCGA TGGCCAAGAA GACACAGTCA
    AAGCCAATGA AGGAGATCCT GACACGTCTT GGAGAATTCG AGTTCATCAA ACTGGTGACG
    TTCGAGGAGA ACGTTATCCT GCGAGAACCG GTCCAAAATT GGCCCACCTG CGACTGCCTG
    GTATCGTTTC ACTCGAAAGG CTTTCCCCTA GAGAAAGCCA TCGAGTATGC CCAGCTTAGG
    AATCCATTTG TGCTGAATAA TCTGCATATG CAGTACGATA TTCAGGACAG GAGAAGAGTG
    TACGCCATTC TGGAGAAGGA GGGTATTGAG ATTCCGCGCT ATGCCGTCCT CGATCGTGAT
    TCGCCGGACC CTAAACACCA TGAGCTTATT GAGTCCGAGG ACCATGTGGA GGTCAATGGT
    ATTACCTTTA ACAAGCCGTT TGTTGAGAAG CCTGTGTCGG CGGAGGACCA CAACATATAC
    ATATATTACC CGACGTCGGC GGGGGGAGGA AGTCAGCGAC TTTTCCGAAA GATCGGCAGC
    CGGAGCAGCG TGTATTCTCC GGAATCAAGG GTGCGAAAGA CAGGATCATT TATCTACGAG
    GACTTTATGC CCACCGATGG CACGGATGTC AAGGTGTACA CCGTGGGACC AGACTACGCC
    CATGCCGAAG CACGTAAAAG TCCCGCGTTG GATGGCAAAG TAGAGCGCGA CAGCGAAGGA
    AAAGAGATTC GCTATCCAGT GATCCTCAAT CATTCCGAGA AGCTAATCTC ACGGAAGGTG
    TGCCTGGCCT TTAAGCAAAC CGTCTGTGGA TTTGATTTGT TGCGAGCCAA CGGAAAGAGC
    TATGTTTGCG ATGTTAACGG CTTTAGCTTC GTGAAGAACT CGAACAAGTA CTATGATGAT
    TGTGCCAAAA TACTGGGCAA CATGATTCTC AGGGAGCTAA CACCTACCCT GCACATACCC
    TGGTCAGTGC CCTTTCAATT AGACGATCCC CCCATTGTGC CCACTACTTT CGGCAAAATG
    ATGGAGCTGC GCTGCGTGGT GGCCGTAATT AGACATGGCG ATCGCACGCC CAAACAAAAA
    ATGAAGGTTG AGGTGCGGCA TCCCAAATTT TTCGAGATCT TCGAGAAGTA CGACGGCTAC
    AAGCTGGGCC ACGTTAAGCT AAAGCGACCC AAACAGCTTC AAGAGATTTT GGACATCGCC
    CGCTTCCTGC TAAGCGAGAT CCACACCAAA GCTCATGCCG AGATTGAGGA AAAGGAAAGC
    AAGCTTGAGC AGCTTAAGAA CGTTCTGGAG ATGTACGGTC ACTTTTCGGG CATAAACCGG
    AAGGTTCAAA TGAAATACCA ACCAAAGGGA CGTCCACGTG GCTCCAGCTC CGATGATACC
    AATCTAGCAG CGGATCAACC AGTGGAACCT TCCCTGGTCC TCATCCTCAA GTGGGGCGGC
    GAACTGACTC CAGCAGGCCG CATCCAGGCG GAGGAGTTGG GTCGCATATT TCGGTGCATG
    TATCCAGGTG GCCAAGGAAG ATCGGATTAC TCGGGCACCC AAGGACTAGG TTTGCTAAGA
    TTGCACTCGA CGTTCCGGCA TGACTTGAAG ATCTACGCCT CAGATGAGGG TCGTGTCCAG
    ATGACGGCTG CCGCTTTTGC AAAGGGTTTG CTGGCCCTGG AAGGAGAACT TACTCCCATT
    TTGGTCCAGA TGGTAAAGAG TGCCAATACA AATGGACTGC TGGACAATGA TTGCGACTCC
    AGCAAGTACC AAAACTTGGC TAAAGGTCGC CTACACGAGC TTATGCAAAA CGATCGAGAG
    TTCTCTAAGG AGGATCGTGA GCTGATTAAT CCTTGCAATA GCAAATCGAT TACCCAGGCT
    CTGGATTTTG TAAAAAATCC TGTTGACTGC TGTCACCACG TACACCTGCT TATTCGCGAG
    CTTCTTCACA TTATCAGCAT CAAAAAGGAT GATCCAAAGA CCAAGGACGC CATCTTATAT
    CACGGCGAGA CGTGGGACCT GATGCGTTGT CGCTGGGAAA AAATTGAGAA GGATTTTAGC
    ACCAAATCGA AGCTGTTTGA CATCTCAAAG ATACCAGATA TATATGATTG CATCAAATAC
    GATCTGCAAC ATAATCAGCA CACGTTGCAA TACGATCAGG CGGAGGAGCT ATACATCTAT
    GCGAAGAATC TGGCTGATAT AGTCATACCA CAGGAGTATG GACTGACGCC ACAGGAGAAA
    CTGGCAATTG GTCAAGGTAT CTGCTCCCCA TTACTGAGAA AGATCAAGGG CGATCTGCAG
    CGAAACATCG ACGAAGTGGA AGACGAGTTT ATGAACCGTT TGAATCCACA TTATAGCCAT
    GGTGTTGCTA GCCCTCAGAG GCATGTTCGT ACAAGGCTCT ATTTTACCAG CGAATCGCAT
    GTTCACTCGC TGCTCACCGT TTTGCGTTAT GGAGGATTGC TCAATGTGGT CACAGATGAG
    CAGTGGCGTC GGGCTATGGA CTATATTTCG ATGGTATCGG AGCTCAACTA TATGTCCCAG
    ATCGTTATCA TGCTCTACGA GGATCCGACC AAAGATCCAA CTTCTGAGGA ACGCTTCCAT
    GTCGAACTGC ACTTTAGTCC GGGTGTTAAT TGCTGTGTGC AAAAGAATCT ACCACCAGGT
    CCGGGATTTC GCCCGCATTC GCACGGCGAT AATGCTTGCA ATGTAAGTTT GCAATCCTCG
    GACGAGTCAA ATCCTGCCAG AATCGAGGAG GAGAATGACT CGAATTCAGG AGAAGAACGG
    GAGGGCAAAA AGCGAGGGAC CTCAGGCCAA AGGAGTACGG ATCGCAGTGC GGAACGCATC
    TCCCCTGCTT TCGGATTCAA TCGATTAGAG CTCAGATCCA AGCAGTTCAA ATCGAAACCT
    ATCCCCATCG GAGCCCATCA CACGGTCAGT GGCCATGAAG CAATGGATCT AGCCAAGCGG
    CTAAACGAGG AGCTGGCATC GCACCAACAG CAGCAAAACC AGCAGCTTCG ACCCATCAGT
    CCGGATATAA GGGCAGTAAC TCCTGACTGC GAACCACGCT CCCGGAGTTT TGAGCAGCGT
    CCAACGTCCG GCGTATGTGC CAAAGAACCG GTAGTTTCTT CTCCTGATTT CGGGGATAAT
    TCTCGGACTC GTTCTTCTGA ATTCGGGGAG ACTTGTCATG CTCGAGTGGG CACTGCTAGT
    AACTCAGATG GGGGCGGGTC TCATCCGAGA GCGGATCATC GCACTGCCTT TCAGATTCGT
    GTTACAAATA GTTTGTCCTT CTTTAAAATT GACTCTTCGA CAAACGAGCT GCCTTTGTCG
    GACATTGATT TTTCGCTGCA CCCACCCACT CCGCAATGCG GTCCCTTGTC GCACAAGCGC
    TTCCACATCT TGACTATGCG GCGGATGGAG AGTTGCGACG ATGGTGCAGA ACTCGAACAG
    GTTCGTCATT TGCCACAGAT CTCGCCGATG GCCACCAATG AGCGACCCTT AAGTTCCTGC
    AATTGCAGTA GCTCAGCGGC CCAAGCGCAT TCGCACTCAA AAAGTCTGAT GGACTTGGCA
    CAAGCGGTGG TTATGACTTC ACCGCAAGAA ACTGGACCGA ATTCGGAGCA AGGTTGTGAC
    CCAACAACGG CGGCTGATCT CTGTCGGGAC AAGGACAAGG CCCTGGCTTC TGGCACATCC
    TCTGCACACA GCAAAGCCAC TAGTAACTCC TGTGGTCAGC TCTCCATGGC AGGTTCTGCT
    CCTGTGGTGA CAGAAAACCC ATTTCGATTC ACCGTTTCGT CATTGGGCTC TGCAGCTACC
    AATACCGCCT GTTTTGTAGG AAGCTTCGAG CCCATTGAAG AGCAGATCAC GTCCATTGTG
    GAGGTAGATT CAAAGCCCCT GCAGCCAGAG CCTCAAGTAG TATATAATCT GCCCACTGTG
    CTCATTACGG GAACAGCAAG TAGCTCAGAA TTGTCGACCA AACTAAATAG CAATATACTA
    CCGACTGTTA CAAACGCTTT TTCTGCAATA GATACCGAAA TAAATGACGA AATAGGTATA
    TCGAAGGAAG TAGGAATAGG GACAATAAGA ATTACAAACA CACATACACC CTGCAATAAG
    GCAACAGTTA CTCGACCAGA TACAAAAATA GCTATAACAA CTGATCCACA AACGGTTACA
    GCAACAGAGA CAGCAGAAGA TACATCAATG GATATACAGC CAAAGATATG TATTGGAAAA
    TGTGCACCTG GGCTGCTTAC ACCAACAGTT ACTACTACCG ATATTTTAAA AGATAGCCAA
    GTCTCGGTGT CGGTCTCGGC ATCGGTGTCA TCGGCCAACG CATCCACGTC GTCGCGTCGC
    CAAAGACACA GTATTGCCGG CCAGATGTCC TATATGAAAA TGTTGGGTTT CGGTGGTTTT
    AGCAAAAAGA TGGCCACCAG CGCAAATAGC CTTTTCAGCA CCGCCGTCAT CAGCGGCAGC
    TCGTCCGCTC CAAATCTTCG CGACATGATA ACGGTCTCCT CTTCCGGATT TGGCGATGTA
    CCACCAATCC GCCCGCTGGA GACACTGCAC AACGCCCTTT CGCTGCGCAA GCTGGACAGT
    TTCTTGCAGG ACATGATCCT GGCGCAGATC TTTAAGACGC CGACAGGGTC CCCGCCGCGG
    GGCTTCTCTA AGAATACCTT GCCAGCGGTT TCCTCGATGA CTCTAACCGC CTCAAATCAG
    ACAGAGGTTG TCGAACATGA GCCGATATCA ACGACCGTAA CGCCCACCTT TGACCGGCGT
    GGCAGCGAGT CCGGATCCAC AGCGGATGCC CATCTGCGTC TTCTTTCGGA GAGCCAGTGC
    CCTAATCTGG ACGATAGCAA CACCGAGCTG CGAGAATCGC TGGCTGGAAA AATGGAGCAA
    TATCCAACAG AGCCCAGCAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21572
    Clone ID Related Accession (Same CDS sequence) NM_001298604.1
    Accession Version NM_001298604.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTGGA CTTGGTTCAA AGACTGGTGG CGTCTCAAGA AGCTCCGATC TCGTCACCAG 
    CGACATAAGA AAAAACTCCA AGCAACCGCG GCGTCCGCTG AAGCTGCGGT GACAGCAAGC
    GCATTTTTGG CCGGCTCTGG GTGCGCGGAA AGTGGTGGTT CCCAGGATCC TCAAACCAAT
    CGTCTCCACA GCTTGGACGC AGTGCCATCT GATGGAACTT TAGCCAGAAG ACGCCATGGT
    AGTCGTGATA GTCTACGCCG GAATCGCTTG CTGCAGCGCC AGCGACGGAG TCAAAGTGCC
    GCAGTTCGCA GTCATAGAAG CCACGACGAC GACGACGATG GGGAGTACGG ACCCTTCAAC
    GTCAGTGATT ACGAGGATTA CGGGCCAAAT CATGGCAGCG ACGAGGAGAG TGATGATTTC
    TGCTACTGTG AAGTCTGCAT GAATGGCGAC ACGGACTTCG GAGACAGCAA TGACGGGATG
    GACTCCGACA CGAGCACCTC TTCCAGCAAC AGCAAGCAGG TGGTTGTCGG CATCTGCGCG
    ATGGCCAAGA AGACACAGTC AAAGCCAATG AAGGAGATCC TGACACGTCT TGGAGAATTC
    GAGTTCATCA AACTGGTGAC GTTCGAGGAG AACGTTATCC TGCGAGAACC GGTCCAAAAT
    TGGCCCACCT GCGACTGCCT GGTATCGTTT CACTCGAAAG GCTTTCCCCT AGAGAAAGCC
    ATCGAGTATG CCCAGCTTAG GAATCCATTT GTGCTGAATA ATCTGCATAT GCAGTACGAT
    ATTCAGGACA GGAGAAGAGT GTACGCCATT CTGGAGAAGG AGGGTATTGA GATTCCGCGC
    TATGCCGTCC TCGATCGTGA TTCGCCGGAC CCTAAACACC ATGAGCTTAT TGAGTCCGAG
    GACCATGTGG AGGTCAATGG TATTACCTTT AACAAGCCGT TTGTTGAGAA GCCTGTGTCG
    GCGGAGGACC ACAACATATA CATATATTAC CCGACGTCGG CGGGGGGAGG AAGTCAGCGA
    CTTTTCCGAA AGATCGGCAG CCGGAGCAGC GTGTATTCTC CGGAATCAAG GGTGCGAAAG
    ACAGGATCAT TTATCTACGA GGACTTTATG CCCACCGATG TGTACTTTTC AGGCACGGAT
    GTCAAGGTGT ACACCGTGGG ACCAGACTAC GCCCATGCCG AAGCACGTAA AAGTCCCGCG
    TTGGATGGCA AAGTAGAGCG CGACAGCGAA GGAAAAGAGA TTCGCTATCC AGTGATCCTC
    AATCATTCCG AGAAGCTAAT CTCACGGAAG GTGTGCCTGG CCTTTAAGCA AACCGTCTGT
    GGATTTGATT TGTTGCGAGC CAACGGAAAG AGCTATGTTT GCGATGTTAA CGGCTTTAGC
    TTCGTGAAGA ACTCGAACAA GTACTATGAT GATTGTGCCA AAATACTGGG CAACATGATT
    CTCAGGGAGC TAACACCTAC CCTGCACATA CCCTGGTCAG TGCCCTTTCA ATTAGACGAT
    CCCCCCATTG TGCCCACTAC TTTCGGCAAA ATGATGGAGC TGCGCTGCGT GGTGGCCGTA
    ATTAGACATG GCGATCGCAC GCCCAAACAA AAAATGAAGG TTGAGGTGCG GCATCCCAAA
    TTTTTCGAGA TCTTCGAGAA GTACGACGGC TACAAGCTGG GCCACGTTAA GCTAAAGCGA
    CCCAAACAGC TTCAAGAGAT TTTGGACATC GCCCGCTTCC TGCTAAGCGA GATCCACACC
    AAAGCTCATG CCGAGATTGA GGAAAAGGAA AGCAAGCTTG AGCAGCTTAA GAACGTTCTG
    GAGATGTACG GTCACTTTTC GGGCATAAAC CGGAAGGTTC AAATGAAATA CCAACCAAAG
    GGACGTCCAC GTGGCTCCAG CTCCGATGAT ACAGCGGATC AACCAGTGGA ACCTTCCCTG
    GTCCTCATCC TCAAGTGGGG CGGCGAACTG ACTCCAGCAG GCCGCATCCA GGCGGAGGAG
    TTGGGTCGCA TATTTCGGTG CATGTATCCA GGTGGCCAAG GAAGATCGGA TTACTCGGGC
    ACCCAAGGAC TAGGTTTGCT AAGATTGCAC TCGACGTTCC GGCATGACTT GAAGATCTAC
    GCCTCAGATG AGGGTCGTGT CCAGATGACG GCTGCCGCTT TTGCAAAGGG TTTGCTGGCC
    CTGGAAGGAG AACTTACTCC CATTTTGGTC CAGATGGTAA AGAGTGCCAA TACAAATGGA
    CTGCTGGACA ATGATTGCGA CTCCAGCAAG TACCAAAACT TGGCTAAAGG TCGCCTACAC
    GAGCTTATGC AAAACGATCG AGAGTTCTCT AAGGAGGATC GTGAGCTGAT TAATCCTTGC
    AATAGCAAAT CGATTACCCA GGCTCTGGAT TTTGTAAAAA ATCCTGTTGA CTGCTGTCAC
    CACGTACACC TGCTTATTCG CGAGCTTCTT CACATTATCA GCATCAAAAA GGATGATCCA
    AAGACCAAGG ACGCCATCTT ATATCACGGC GAGACGTGGG ACCTGATGCG TTGTCGCTGG
    GAAAAAATTG AGAAGGATTT TAGCACCAAA TCGAAGCTGT TTGACATCTC AAAGATACCA
    GATATATATG ATTGCATCAA ATACGATCTG CAACATAATC AGCACACGTT GCAATACGAT
    CAGGCGGAGG AGCTATACAT CTATGCGAAG AATCTGGCTG ATATAGTCAT ACCACAGGAG
    TATGGACTGA CGCCACAGGA GAAACTGGCA ATTGGTCAAG GTATCTGCTC CCCATTACTG
    AGAAAGATCA AGGGCGATCT GCAGCGAAAC ATCGACGAAG TGGAAGACGA GTTTATGAAC
    CGTTTGAATC CACATTATAG CCATGGTGTT GCTAGCCCTC AGAGGCATGT TCGTACAAGG
    CTCTATTTTA CCAGCGAATC GCATGTTCAC TCGCTGCTCA CCGTTTTGCG TTATGGAGGA
    TTGCTCAATG TGGTCACAGA TGAGCAGTGG CGTCGGGCTA TGGACTATAT TTCGATGGTA
    TCGGAGCTCA ACTATATGTC CCAGATCGTT ATCATGCTCT ACGAGGATCC GACCAAAGAT
    CCAACTTCTG AGGAACGCTT CCATGTCGAA CTGCACTTTA GTCCGGGTGT TAATTGCTGT
    GTGCAAAAGA ATCTACCACC AGGTCCGGGA TTTCGCCCGC ATTCGCACGG CGATAATGCT
    TGCAATGTAA GTTTGCAATC CTCGGACGAG TCAAATCCTG CCAGAATCGA GGAGGAGAAT
    GACTCGAATT CAGGAGAAGA ACGGGAGGGC AAAAAGCGAG GGACCTCAGG CCAAAGGAGT
    ACGGATCGCA GTGCGGAACG CATCTCCCCT GCTTTCGGAT TCAATCGATT AGAGCTCAGA
    TCCAAGCAGT TCAAATCGAA ACCTATCCCC ATCGGAGCCC ATCACACGGT CAGTGGCCAT
    GAAGCAATGG ATCTAGCCAA GCGGCTAAAC GAGGAGCTGG CATCGCACCA ACAGCAGCAA
    AACCAGCAGC TTCGACCCAT CAGTCCGGAT ATAAGGGCAG TAACTCCTGA CTGCGAACCA
    CGCTCCCGGA GTTTTGAGCA GCGTCCAACG TCCGGCGTAT GTGCCAAAGA ACCGGATAGC
    CAAGTCTCGG TGTCGGTCTC GGCATCGGTG TCATCGGCCA ACGCATCCAC GTCGTCGCGT
    CGCCAAAGAC ACAGTATTGC CGGCCAGATG TCCTATATGA AAATGTTGGG TTTCGGTGGT
    TTTAGCAAAA AGATGGCCAC CAGCGCAAAT AGCCTTTTCA GCACCGCCGT CATCAGCGGC
    AGCTCGTCCG CTCCAAATCT TCGCGACATG ATAACGGTCT CCTCTTCCGG ATTTGGCGAT
    GTACCACCAA TCCGCCCGCT GGAGACACTG CACAACGCCC TTTCGCTGCG CAAGCTGGAC
    AGTTTCTTGC AGGACATGAT CCTGGCGCAG ATCTTTAAGA CGCCGACAGG GTCCCCGCCG
    CGGGGCTTCT CTAAGAATAC CTTGCCAGCG GTTTCCTCGA TGACTCTAAC CGCCTCAAAT
    CAGACAGAGG TTGTCGAACA TGAGCCGATA TCAACGACCG TAACGCCCAC CTTTGACCGG
    CGTGGCAGCG AGTCCGGATC CACAGCGGAT GCCCATCTGC GTCTTCTTTC GGAGAGCCAG
    TGCCCTAATC TGGACGATAG CAACACCGAG CTGCGAGAAT CGCTGGCTGG AAAAATGGAG
    CGTAAGTGCC GAAAAAAGTC AGTGGTCGAG TGGCAGTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285533.1
    CDS384..4625
    Translation

    Target ORF information:

    RefSeq Version NM_001298604.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298604.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAGTGGA CTTGGTTCAA AGACTGGTGG CGTCTCAAGA AGCTCCGATC TCGTCACCAG 
    CGACATAAGA AAAAACTCCA AGCAACCGCG GCGTCCGCTG AAGCTGCGGT GACAGCAAGC
    GCATTTTTGG CCGGCTCTGG GTGCGCGGAA AGTGGTGGTT CCCAGGATCC TCAAACCAAT
    CGTCTCCACA GCTTGGACGC AGTGCCATCT GATGGAACTT TAGCCAGAAG ACGCCATGGT
    AGTCGTGATA GTCTACGCCG GAATCGCTTG CTGCAGCGCC AGCGACGGAG TCAAAGTGCC
    GCAGTTCGCA GTCATAGAAG CCACGACGAC GACGACGATG GGGAGTACGG ACCCTTCAAC
    GTCAGTGATT ACGAGGATTA CGGGCCAAAT CATGGCAGCG ACGAGGAGAG TGATGATTTC
    TGCTACTGTG AAGTCTGCAT GAATGGCGAC ACGGACTTCG GAGACAGCAA TGACGGGATG
    GACTCCGACA CGAGCACCTC TTCCAGCAAC AGCAAGCAGG TGGTTGTCGG CATCTGCGCG
    ATGGCCAAGA AGACACAGTC AAAGCCAATG AAGGAGATCC TGACACGTCT TGGAGAATTC
    GAGTTCATCA AACTGGTGAC GTTCGAGGAG AACGTTATCC TGCGAGAACC GGTCCAAAAT
    TGGCCCACCT GCGACTGCCT GGTATCGTTT CACTCGAAAG GCTTTCCCCT AGAGAAAGCC
    ATCGAGTATG CCCAGCTTAG GAATCCATTT GTGCTGAATA ATCTGCATAT GCAGTACGAT
    ATTCAGGACA GGAGAAGAGT GTACGCCATT CTGGAGAAGG AGGGTATTGA GATTCCGCGC
    TATGCCGTCC TCGATCGTGA TTCGCCGGAC CCTAAACACC ATGAGCTTAT TGAGTCCGAG
    GACCATGTGG AGGTCAATGG TATTACCTTT AACAAGCCGT TTGTTGAGAA GCCTGTGTCG
    GCGGAGGACC ACAACATATA CATATATTAC CCGACGTCGG CGGGGGGAGG AAGTCAGCGA
    CTTTTCCGAA AGATCGGCAG CCGGAGCAGC GTGTATTCTC CGGAATCAAG GGTGCGAAAG
    ACAGGATCAT TTATCTACGA GGACTTTATG CCCACCGATG TGTACTTTTC AGGCACGGAT
    GTCAAGGTGT ACACCGTGGG ACCAGACTAC GCCCATGCCG AAGCACGTAA AAGTCCCGCG
    TTGGATGGCA AAGTAGAGCG CGACAGCGAA GGAAAAGAGA TTCGCTATCC AGTGATCCTC
    AATCATTCCG AGAAGCTAAT CTCACGGAAG GTGTGCCTGG CCTTTAAGCA AACCGTCTGT
    GGATTTGATT TGTTGCGAGC CAACGGAAAG AGCTATGTTT GCGATGTTAA CGGCTTTAGC
    TTCGTGAAGA ACTCGAACAA GTACTATGAT GATTGTGCCA AAATACTGGG CAACATGATT
    CTCAGGGAGC TAACACCTAC CCTGCACATA CCCTGGTCAG TGCCCTTTCA ATTAGACGAT
    CCCCCCATTG TGCCCACTAC TTTCGGCAAA ATGATGGAGC TGCGCTGCGT GGTGGCCGTA
    ATTAGACATG GCGATCGCAC GCCCAAACAA AAAATGAAGG TTGAGGTGCG GCATCCCAAA
    TTTTTCGAGA TCTTCGAGAA GTACGACGGC TACAAGCTGG GCCACGTTAA GCTAAAGCGA
    CCCAAACAGC TTCAAGAGAT TTTGGACATC GCCCGCTTCC TGCTAAGCGA GATCCACACC
    AAAGCTCATG CCGAGATTGA GGAAAAGGAA AGCAAGCTTG AGCAGCTTAA GAACGTTCTG
    GAGATGTACG GTCACTTTTC GGGCATAAAC CGGAAGGTTC AAATGAAATA CCAACCAAAG
    GGACGTCCAC GTGGCTCCAG CTCCGATGAT ACAGCGGATC AACCAGTGGA ACCTTCCCTG
    GTCCTCATCC TCAAGTGGGG CGGCGAACTG ACTCCAGCAG GCCGCATCCA GGCGGAGGAG
    TTGGGTCGCA TATTTCGGTG CATGTATCCA GGTGGCCAAG GAAGATCGGA TTACTCGGGC
    ACCCAAGGAC TAGGTTTGCT AAGATTGCAC TCGACGTTCC GGCATGACTT GAAGATCTAC
    GCCTCAGATG AGGGTCGTGT CCAGATGACG GCTGCCGCTT TTGCAAAGGG TTTGCTGGCC
    CTGGAAGGAG AACTTACTCC CATTTTGGTC CAGATGGTAA AGAGTGCCAA TACAAATGGA
    CTGCTGGACA ATGATTGCGA CTCCAGCAAG TACCAAAACT TGGCTAAAGG TCGCCTACAC
    GAGCTTATGC AAAACGATCG AGAGTTCTCT AAGGAGGATC GTGAGCTGAT TAATCCTTGC
    AATAGCAAAT CGATTACCCA GGCTCTGGAT TTTGTAAAAA ATCCTGTTGA CTGCTGTCAC
    CACGTACACC TGCTTATTCG CGAGCTTCTT CACATTATCA GCATCAAAAA GGATGATCCA
    AAGACCAAGG ACGCCATCTT ATATCACGGC GAGACGTGGG ACCTGATGCG TTGTCGCTGG
    GAAAAAATTG AGAAGGATTT TAGCACCAAA TCGAAGCTGT TTGACATCTC AAAGATACCA
    GATATATATG ATTGCATCAA ATACGATCTG CAACATAATC AGCACACGTT GCAATACGAT
    CAGGCGGAGG AGCTATACAT CTATGCGAAG AATCTGGCTG ATATAGTCAT ACCACAGGAG
    TATGGACTGA CGCCACAGGA GAAACTGGCA ATTGGTCAAG GTATCTGCTC CCCATTACTG
    AGAAAGATCA AGGGCGATCT GCAGCGAAAC ATCGACGAAG TGGAAGACGA GTTTATGAAC
    CGTTTGAATC CACATTATAG CCATGGTGTT GCTAGCCCTC AGAGGCATGT TCGTACAAGG
    CTCTATTTTA CCAGCGAATC GCATGTTCAC TCGCTGCTCA CCGTTTTGCG TTATGGAGGA
    TTGCTCAATG TGGTCACAGA TGAGCAGTGG CGTCGGGCTA TGGACTATAT TTCGATGGTA
    TCGGAGCTCA ACTATATGTC CCAGATCGTT ATCATGCTCT ACGAGGATCC GACCAAAGAT
    CCAACTTCTG AGGAACGCTT CCATGTCGAA CTGCACTTTA GTCCGGGTGT TAATTGCTGT
    GTGCAAAAGA ATCTACCACC AGGTCCGGGA TTTCGCCCGC ATTCGCACGG CGATAATGCT
    TGCAATGTAA GTTTGCAATC CTCGGACGAG TCAAATCCTG CCAGAATCGA GGAGGAGAAT
    GACTCGAATT CAGGAGAAGA ACGGGAGGGC AAAAAGCGAG GGACCTCAGG CCAAAGGAGT
    ACGGATCGCA GTGCGGAACG CATCTCCCCT GCTTTCGGAT TCAATCGATT AGAGCTCAGA
    TCCAAGCAGT TCAAATCGAA ACCTATCCCC ATCGGAGCCC ATCACACGGT CAGTGGCCAT
    GAAGCAATGG ATCTAGCCAA GCGGCTAAAC GAGGAGCTGG CATCGCACCA ACAGCAGCAA
    AACCAGCAGC TTCGACCCAT CAGTCCGGAT ATAAGGGCAG TAACTCCTGA CTGCGAACCA
    CGCTCCCGGA GTTTTGAGCA GCGTCCAACG TCCGGCGTAT GTGCCAAAGA ACCGGATAGC
    CAAGTCTCGG TGTCGGTCTC GGCATCGGTG TCATCGGCCA ACGCATCCAC GTCGTCGCGT
    CGCCAAAGAC ACAGTATTGC CGGCCAGATG TCCTATATGA AAATGTTGGG TTTCGGTGGT
    TTTAGCAAAA AGATGGCCAC CAGCGCAAAT AGCCTTTTCA GCACCGCCGT CATCAGCGGC
    AGCTCGTCCG CTCCAAATCT TCGCGACATG ATAACGGTCT CCTCTTCCGG ATTTGGCGAT
    GTACCACCAA TCCGCCCGCT GGAGACACTG CACAACGCCC TTTCGCTGCG CAAGCTGGAC
    AGTTTCTTGC AGGACATGAT CCTGGCGCAG ATCTTTAAGA CGCCGACAGG GTCCCCGCCG
    CGGGGCTTCT CTAAGAATAC CTTGCCAGCG GTTTCCTCGA TGACTCTAAC CGCCTCAAAT
    CAGACAGAGG TTGTCGAACA TGAGCCGATA TCAACGACCG TAACGCCCAC CTTTGACCGG
    CGTGGCAGCG AGTCCGGATC CACAGCGGAT GCCCATCTGC GTCTTCTTTC GGAGAGCCAG
    TGCCCTAATC TGGACGATAG CAACACCGAG CTGCGAGAAT CGCTGGCTGG AAAAATGGAG
    CGTAAGTGCC GAAAAAAGTC AGTGGTCGAG TGGCAGTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21573
    Clone ID Related Accession (Same CDS sequence) NM_001298603.1
    Accession Version NM_001298603.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (1) G0196, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTGGA CTTGGTTCAA AGACTGGTGG CGTCTCAAGA AGCTCCGATC TCGTCACCAG 
    CGACATAAGA AAAAACTCCA AGCAACCGCG GCGTCCGCTG AAGCTGCGGT GACAGCAAGC
    GCATTTTTGG CCGGCTCTGG GTGCGCGGAA AGTGGTGGTT CCCAGGATCC TCAAACCAAT
    CGTCTCCACA GCTTGGACGC AGTGCCATCT GATGGAACTT TAGCCAGAAG ACGCCATGGT
    AGTCGTGATA GTCTACGCCG GAATCGCTTG CTGCAGCGCC AGCGACGGAG TCAAAGTGCC
    GCAGTTCGCA GTCATAGAAG CCACGACGAC GACGACGATG GGGAGTACGG ACCCTTCAAC
    GTCAGTGATT ACGAGGATTA CGGGCCAAAT CATGGCAGCG ACGAGGAGAG TGATGATTTC
    TGCTACTGTG AAGTCTGCAT GAATGGCGAC ACGGACTTCG GAGACAGCAA TGACGGGATG
    GACTCCGACA CGAGCACCTC TTCCAGCAAC AGCAAGCAGG TGGTTGTCGG CATCTGCGCG
    ATGGCCAAGA AGACACAGTC AAAGCCAATG AAGGAGATCC TGACACGTCT TGGAGAATTC
    GAGTTCATCA AACTGGTGAC GTTCGAGGAG AACGTTATCC TGCGAGAACC GGTCCAAAAT
    TGGCCCACCT GCGACTGCCT GGTATCGTTT CACTCGAAAG GCTTTCCCCT AGAGAAAGCC
    ATCGAGTATG CCCAGCTTAG GAATCCATTT GTGCTGAATA ATCTGCATAT GCAGTACGAT
    ATTCAGGACA GGAGAAGAGT GTACGCCATT CTGGAGAAGG AGGGTATTGA GATTCCGCGC
    TATGCCGTCC TCGATCGTGA TTCGCCGGAC CCTAAACACC ATGAGCTTAT TGAGTCCGAG
    GACCATGTGG AGGTCAATGG TATTACCTTT AACAAGCCGT TTGTTGAGAA GCCTGTGTCG
    GCGGAGGACC ACAACATATA CATATATTAC CCGACGTCGG CGGGGGGAGG AAGTCAGCGA
    CTTTTCCGAA AGATCGGCAG CCGGAGCAGC GTGTATTCTC CGGAATCAAG GGTGCGAAAG
    ACAGGATCAT TTATCTACGA GGACTTTATG CCCACCGATG TGTACTTTTC AGGCACGGAT
    GTCAAGGTGT ACACCGTGGG ACCAGACTAC GCCCATGCCG AAGCACGTAA AAGTCCCGCG
    TTGGATGGCA AAGTAGAGCG CGACAGCGAA GGAAAAGAGA TTCGCTATCC AGTGATCCTC
    AATCATTCCG AGAAGCTAAT CTCACGGAAG GTGTGCCTGG CCTTTAAGCA AACCGTCTGT
    GGATTTGATT TGTTGCGAGC CAACGGAAAG AGCTATGTTT GCGATGTTAA CGGCTTTAGC
    TTCGTGAAGA ACTCGAACAA GTACTATGAT GATTGTGCCA AAATACTGGG CAACATGATT
    CTCAGGGAGC TAACACCTAC CCTGCACATA CCCTGGTCAG TGCCCTTTCA ATTAGACGAT
    CCCCCCATTG TGCCCACTAC TTTCGGCAAA ATGATGGAGC TGCGCTGCGT GGTGGCCGTA
    ATTAGACATG GCGATCGCAC GCCCAAACAA AAAATGAAGG TTGAGGTGCG GCATCCCAAA
    TTTTTCGAGA TCTTCGAGAA GTACGACGGC TACAAGCTGG GCCACGTTAA GCTAAAGCGA
    CCCAAACAGC TTCAAGAGAT TTTGGACATC GCCCGCTTCC TGCTAAGCGA GATCCACACC
    AAAGCTCATG CCGAGATTGA GGAAAAGGAA AGCAAGCTTG AGCAGCTTAA GAACGTTCTG
    GAGATGTACG GTCACTTTTC GGGCATAAAC CGGAAGGTTC AAATGAAATA CCAACCAAAG
    GGACGTCCAC GTGGCTCCAG CTCCGATGAT ACGGATCAAC CAGTGGAACC TTCCCTGGTC
    CTCATCCTCA AGTGGGGCGG CGAACTGACT CCAGCAGGCC GCATCCAGGC GGAGGAGTTG
    GGTCGCATAT TTCGGTGCAT GTATCCAGGT GGCCAAGGAA GATCGGATTA CTCGGGCACC
    CAAGGACTAG GTTTGCTAAG ATTGCACTCG ACGTTCCGGC ATGACTTGAA GATCTACGCC
    TCAGATGAGG GTCGTGTCCA GATGACGGCT GCCGCTTTTG CAAAGGGTTT GCTGGCCCTG
    GAAGGAGAAC TTACTCCCAT TTTGGTCCAG ATGGTAAAGA GTGCCAATAC AAATGGACTG
    CTGGACAATG ATTGCGACTC CAGCAAGTAC CAAAACTTGG CTAAAGGTCG CCTACACGAG
    CTTATGCAAA ACGATCGAGA GTTCTCTAAG GAGGATCGTG AGCTGATTAA TCCTTGCAAT
    AGCAAATCGA TTACCCAGGC TCTGGATTTT GTAAAAAATC CTGTTGACTG CTGTCACCAC
    GTACACCTGC TTATTCGCGA GCTTCTTCAC ATTATCAGCA TCAAAAAGGA TGATCCAAAG
    ACCAAGGACG CCATCTTATA TCACGGCGAG ACGTGGGACC TGATGCGTTG TCGCTGGGAA
    AAAATTGAGA AGGATTTTAG CACCAAATCG AAGCTGTTTG ACATCTCAAA GATACCAGAT
    ATATATGATT GCATCAAATA CGATCTGCAA CATAATCAGC ACACGTTGCA ATACGATCAG
    GCGGAGGAGC TATACATCTA TGCGAAGAAT CTGGCTGATA TAGTCATACC ACAGGAGTAT
    GGACTGACGC CACAGGAGAA ACTGGCAATT GGTCAAGGTA TCTGCTCCCC ATTACTGAGA
    AAGATCAAGG GCGATCTGCA GCGAAACATC GACGAAGTGG AAGACGAGTT TATGAACCGT
    TTGAATCCAC ATTATAGCCA TGGTGTTGCT AGCCCTCAGA GGCATGTTCG TACAAGGCTC
    TATTTTACCA GCGAATCGCA TGTTCACTCG CTGCTCACCG TTTTGCGTTA TGGAGGATTG
    CTCAATGTGG TCACAGATGA GCAGTGGCGT CGGGCTATGG ACTATATTTC GATGGTATCG
    GAGCTCAACT ATATGTCCCA GATCGTTATC ATGCTCTACG AGGATCCGAC CAAAGATCCA
    ACTTCTGAGG AACGCTTCCA TGTCGAACTG CACTTTAGTC CGGGTGTTAA TTGCTGTGTG
    CAAAAGAATC TACCACCAGG TCCGGGATTT CGCCCGCATT CGCACGGCGA TAATGCTTGC
    AATGTAAGTT TGCAATCCTC GGACGAGTCA AATCCTGCCA GAATCGAGGA GGAGAATGAC
    TCGAATTCAG GAGAAGAACG GGAGGGCAAA AAGCGAGGGA CCTCAGGCCA AAGGAGTACG
    GATCGCAGTG CGGAACGCAT CTCCCCTGCT TTCGGATTCA ATCGATTAGA GCTCAGATCC
    AAGCAGTTCA AATCGAAACC TATCCCCATC GGAGCCCATC ACACGGTCAG TGGCCATGAA
    GCAATGGATC TAGCCAAGCG GCTAAACGAG GAGCTGGCAT CGCACCAACA GCAGCAAAAC
    CAGCAGCTTC GACCCATCAG TCCGGATATA AGGGCAGTAA CTCCTGACTG CGAACCACGC
    TCCCGGAGTT TTGAGCAGCG TCCAACGTCC GGCGTATGTG CCAAAGAACC GGGATTTGGC
    GATGTACCAC CAATCCGCCC GCTGGAGACA CTGCACAACG CCCTTTCGCT GCGCAAGCTG
    GACAGTTTCT TGCAGGACAT GATCCTGGCG CAGATCTTTA AGACGCCGAC AGGGTCCCCG
    CCGCGGGGCT TCTCTAAGAA TACCTTGCCA GCGGTTTCCT CGATGACTCT AACCGCCTCA
    AATCAGACAG AGGTTGTCGA ACATGAGCCG ATATCAACGA CCGTAACGCC CACCTTTGAC
    CGGCGTGGCA GCGAGTCCGG ATCCACAGCG GATGCCCATC TGCGTCTTCT TTCGGAGAGC
    CAGTGCCCTA ATCTGGACGA TAGCAACACC GAGCTGCGAG AATCGCTGGC TGGAAAAATG
    GAGCGTAAGT GCCGAAAAAA GTCAGTGGTC GAGTGGCAGT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285532.1
    CDS384..4388
    Translation

    Target ORF information:

    RefSeq Version NM_001298603.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (1) G0196 (l(1)G0196), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTGGA CTTGGTTCAA AGACTGGTGG CGTCTCAAGA AGCTCCGATC TCGTCACCAG 
    CGACATAAGA AAAAACTCCA AGCAACCGCG GCGTCCGCTG AAGCTGCGGT GACAGCAAGC
    GCATTTTTGG CCGGCTCTGG GTGCGCGGAA AGTGGTGGTT CCCAGGATCC TCAAACCAAT
    CGTCTCCACA GCTTGGACGC AGTGCCATCT GATGGAACTT TAGCCAGAAG ACGCCATGGT
    AGTCGTGATA GTCTACGCCG GAATCGCTTG CTGCAGCGCC AGCGACGGAG TCAAAGTGCC
    GCAGTTCGCA GTCATAGAAG CCACGACGAC GACGACGATG GGGAGTACGG ACCCTTCAAC
    GTCAGTGATT ACGAGGATTA CGGGCCAAAT CATGGCAGCG ACGAGGAGAG TGATGATTTC
    TGCTACTGTG AAGTCTGCAT GAATGGCGAC ACGGACTTCG GAGACAGCAA TGACGGGATG
    GACTCCGACA CGAGCACCTC TTCCAGCAAC AGCAAGCAGG TGGTTGTCGG CATCTGCGCG
    ATGGCCAAGA AGACACAGTC AAAGCCAATG AAGGAGATCC TGACACGTCT TGGAGAATTC
    GAGTTCATCA AACTGGTGAC GTTCGAGGAG AACGTTATCC TGCGAGAACC GGTCCAAAAT
    TGGCCCACCT GCGACTGCCT GGTATCGTTT CACTCGAAAG GCTTTCCCCT AGAGAAAGCC
    ATCGAGTATG CCCAGCTTAG GAATCCATTT GTGCTGAATA ATCTGCATAT GCAGTACGAT
    ATTCAGGACA GGAGAAGAGT GTACGCCATT CTGGAGAAGG AGGGTATTGA GATTCCGCGC
    TATGCCGTCC TCGATCGTGA TTCGCCGGAC CCTAAACACC ATGAGCTTAT TGAGTCCGAG
    GACCATGTGG AGGTCAATGG TATTACCTTT AACAAGCCGT TTGTTGAGAA GCCTGTGTCG
    GCGGAGGACC ACAACATATA CATATATTAC CCGACGTCGG CGGGGGGAGG AAGTCAGCGA
    CTTTTCCGAA AGATCGGCAG CCGGAGCAGC GTGTATTCTC CGGAATCAAG GGTGCGAAAG
    ACAGGATCAT TTATCTACGA GGACTTTATG CCCACCGATG TGTACTTTTC AGGCACGGAT
    GTCAAGGTGT ACACCGTGGG ACCAGACTAC GCCCATGCCG AAGCACGTAA AAGTCCCGCG
    TTGGATGGCA AAGTAGAGCG CGACAGCGAA GGAAAAGAGA TTCGCTATCC AGTGATCCTC
    AATCATTCCG AGAAGCTAAT CTCACGGAAG GTGTGCCTGG CCTTTAAGCA AACCGTCTGT
    GGATTTGATT TGTTGCGAGC CAACGGAAAG AGCTATGTTT GCGATGTTAA CGGCTTTAGC
    TTCGTGAAGA ACTCGAACAA GTACTATGAT GATTGTGCCA AAATACTGGG CAACATGATT
    CTCAGGGAGC TAACACCTAC CCTGCACATA CCCTGGTCAG TGCCCTTTCA ATTAGACGAT
    CCCCCCATTG TGCCCACTAC TTTCGGCAAA ATGATGGAGC TGCGCTGCGT GGTGGCCGTA
    ATTAGACATG GCGATCGCAC GCCCAAACAA AAAATGAAGG TTGAGGTGCG GCATCCCAAA
    TTTTTCGAGA TCTTCGAGAA GTACGACGGC TACAAGCTGG GCCACGTTAA GCTAAAGCGA
    CCCAAACAGC TTCAAGAGAT TTTGGACATC GCCCGCTTCC TGCTAAGCGA GATCCACACC
    AAAGCTCATG CCGAGATTGA GGAAAAGGAA AGCAAGCTTG AGCAGCTTAA GAACGTTCTG
    GAGATGTACG GTCACTTTTC GGGCATAAAC CGGAAGGTTC AAATGAAATA CCAACCAAAG
    GGACGTCCAC GTGGCTCCAG CTCCGATGAT ACGGATCAAC CAGTGGAACC TTCCCTGGTC
    CTCATCCTCA AGTGGGGCGG CGAACTGACT CCAGCAGGCC GCATCCAGGC GGAGGAGTTG
    GGTCGCATAT TTCGGTGCAT GTATCCAGGT GGCCAAGGAA GATCGGATTA CTCGGGCACC
    CAAGGACTAG GTTTGCTAAG ATTGCACTCG ACGTTCCGGC ATGACTTGAA GATCTACGCC
    TCAGATGAGG GTCGTGTCCA GATGACGGCT GCCGCTTTTG CAAAGGGTTT GCTGGCCCTG
    GAAGGAGAAC TTACTCCCAT TTTGGTCCAG ATGGTAAAGA GTGCCAATAC AAATGGACTG
    CTGGACAATG ATTGCGACTC CAGCAAGTAC CAAAACTTGG CTAAAGGTCG CCTACACGAG
    CTTATGCAAA ACGATCGAGA GTTCTCTAAG GAGGATCGTG AGCTGATTAA TCCTTGCAAT
    AGCAAATCGA TTACCCAGGC TCTGGATTTT GTAAAAAATC CTGTTGACTG CTGTCACCAC
    GTACACCTGC TTATTCGCGA GCTTCTTCAC ATTATCAGCA TCAAAAAGGA TGATCCAAAG
    ACCAAGGACG CCATCTTATA TCACGGCGAG ACGTGGGACC TGATGCGTTG TCGCTGGGAA
    AAAATTGAGA AGGATTTTAG CACCAAATCG AAGCTGTTTG ACATCTCAAA GATACCAGAT
    ATATATGATT GCATCAAATA CGATCTGCAA CATAATCAGC ACACGTTGCA ATACGATCAG
    GCGGAGGAGC TATACATCTA TGCGAAGAAT CTGGCTGATA TAGTCATACC ACAGGAGTAT
    GGACTGACGC CACAGGAGAA ACTGGCAATT GGTCAAGGTA TCTGCTCCCC ATTACTGAGA
    AAGATCAAGG GCGATCTGCA GCGAAACATC GACGAAGTGG AAGACGAGTT TATGAACCGT
    TTGAATCCAC ATTATAGCCA TGGTGTTGCT AGCCCTCAGA GGCATGTTCG TACAAGGCTC
    TATTTTACCA GCGAATCGCA TGTTCACTCG CTGCTCACCG TTTTGCGTTA TGGAGGATTG
    CTCAATGTGG TCACAGATGA GCAGTGGCGT CGGGCTATGG ACTATATTTC GATGGTATCG
    GAGCTCAACT ATATGTCCCA GATCGTTATC ATGCTCTACG AGGATCCGAC CAAAGATCCA
    ACTTCTGAGG AACGCTTCCA TGTCGAACTG CACTTTAGTC CGGGTGTTAA TTGCTGTGTG
    CAAAAGAATC TACCACCAGG TCCGGGATTT CGCCCGCATT CGCACGGCGA TAATGCTTGC
    AATGTAAGTT TGCAATCCTC GGACGAGTCA AATCCTGCCA GAATCGAGGA GGAGAATGAC
    TCGAATTCAG GAGAAGAACG GGAGGGCAAA AAGCGAGGGA CCTCAGGCCA AAGGAGTACG
    GATCGCAGTG CGGAACGCAT CTCCCCTGCT TTCGGATTCA ATCGATTAGA GCTCAGATCC
    AAGCAGTTCA AATCGAAACC TATCCCCATC GGAGCCCATC ACACGGTCAG TGGCCATGAA
    GCAATGGATC TAGCCAAGCG GCTAAACGAG GAGCTGGCAT CGCACCAACA GCAGCAAAAC
    CAGCAGCTTC GACCCATCAG TCCGGATATA AGGGCAGTAA CTCCTGACTG CGAACCACGC
    TCCCGGAGTT TTGAGCAGCG TCCAACGTCC GGCGTATGTG CCAAAGAACC GGGATTTGGC
    GATGTACCAC CAATCCGCCC GCTGGAGACA CTGCACAACG CCCTTTCGCT GCGCAAGCTG
    GACAGTTTCT TGCAGGACAT GATCCTGGCG CAGATCTTTA AGACGCCGAC AGGGTCCCCG
    CCGCGGGGCT TCTCTAAGAA TACCTTGCCA GCGGTTTCCT CGATGACTCT AACCGCCTCA
    AATCAGACAG AGGTTGTCGA ACATGAGCCG ATATCAACGA CCGTAACGCC CACCTTTGAC
    CGGCGTGGCA GCGAGTCCGG ATCCACAGCG GATGCCCATC TGCGTCTTCT TTCGGAGAGC
    CAGTGCCCTA ATCTGGACGA TAGCAACACC GAGCTGCGAG AATCGCTGGC TGGAAAAATG
    GAGCGTAAGT GCCGAAAAAA GTCAGTGGTC GAGTGGCAGT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.