Ssu72 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ssu72
Entrez Gene ID	32973
Full Name	Ssu72 CTD phosphatase
Synonyms	BEST:CK01830,CG14216,Dmel\CG14216,dSsu72
General protein information	Preferred Names Ssu72 CTD phosphatase Names CG14216 gene product from transcript CG14216-RA CG14216-PA CG14216-PB CG14216-PC Ssu72 ortholog Ssu72-PA Ssu72-PB Ssu72-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18D13-18D13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298526.1	NP_001285455.1	Ssu72 CTD phosphatase, isoform C
NM_134498.4	NP_608342.1	Ssu72 CTD phosphatase, isoform A
NM_001272796.2	NP_001259725.1	Ssu72 CTD phosphatase, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ssu72	NP_957022.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011470	XP_317836.3
Schizosaccharomyces pombe (fission yeast)	ssu72	NP_594076.1
Oryza sativa (rice)	Os12g0168200	NP_001066253.1
Xenopus tropicalis (tropical clawed frog)	ssu72	NP_001016264.1
	SSU72	XP_001094964.1
Mus musculus (house mouse)	Ssu72	NP_081175.2
Gallus gallus (chicken)	SSU72	NP_001007876.1
Drosophila melanogaster (fruit fly)	Ssu72	NP_608342.1
Caenorhabditis elegans (roundworm)	ssup-72	NP_495386.3
Kluyveromyces lactis	KLLA0D19514g	XP_453928.1
Bos taurus (cattle)	SSU72	NP_001069817.1
Homo sapiens (human)	SSU72	NP_054907.1
Canis lupus familiaris (dog)	SSU72	NP_001239254.1
Eremothecium gossypii	AGOS_ABL190W	NP_982757.1
Magnaporthe oryzae (rice blast fungus)	MGG_03704	XP_003716177.1
Neurospora crassa	NCU03114	XP_964973.1
Arabidopsis thaliana (thale cress)	AT1G73820	NP_177523.1
Pan troglodytes (chimpanzee)	SSU72	XP_001147261.1
Rattus norvegicus (Norway rat)	Ssu72	NP_001020828.1
Saccharomyces cerevisiae (baker's yeast)	SSU72	NP_014177.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Determined are the kinetic and structural properties of Drosophila Ssu72-symplekin in complex with the C-terminal domain of RNA polymerase II peptide with consecutive phosphorylated Thr4 and Ser5. [Ssu72]
Title: novel modifications on C-terminal domain of RNA polymerase II can fine-tune the phosphatase activity of Ssu72.

The Ssu72 structure exhibits a core fold with a similar topology to that of LMWPTPs [low-molecular-mass PTPs (protein tyrosine phosphatases)].
Title: Crystal structure of Ssu72, an essential eukaryotic phosphatase specific for the C-terminal domain of RNA polymerase II, in complex with a transition state analogue.

The following Ssu72 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ssu72 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02160	NM_001298526.1 Latest version!	Drosophila melanogaster Ssu72 CTD phosphatase (Ssu72), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa02160	NM_134498.4 Latest version!	Drosophila melanogaster Ssu72 CTD phosphatase (Ssu72), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa03170	NM_001272796.2 Latest version!	Drosophila melanogaster Ssu72 CTD phosphatase (Ssu72), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ssu72 ( NM_001298526.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285455.1 Drosophila melanogaster Ssu72 CTD phosphatase (Ssu72), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa02160
Clone ID Related Accession (Same CDS sequence)	NM_001298526.1 , NM_134498.4
Accession Version	NM_001298526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ssu72 CTD phosphatase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGATC CTAGCAAGCT CGCCGTCGCC GTAGTCTGTT CCTCGAACAT GAATCGCTCA 
ATGGAGGCGC ACAACTTTCT GGCCAAGAAG GGTTTCAATG TGCGCTCCTA CGGCACCGGC 
GAGCGCGTCA AGCTACCGGG CATGGCGTTC GACAAGCCGA ATGTTTACGA GTTTGGCACC 
AAATACGAGG ACATCTACCG CGACCTGGAG TCCAAGGACA AGGAGTTCTA CACACAGAAC 
GGCCTGCTAC ACATGCTCGA CCGCAATCGT CGCATAAAGA AGTGCCCGGA ACGGTTTCAG 
GACACCAAAG AGCAGTTCGA CATCATCGTC ACTGTGGAAG AGCGCGTCTA CGACCTGGTG 
GTGATGCACA TGGAGTCCAT GGAATCCGTG GACAACCGAC CCGTGCACGT TCTCAACGTG 
GATGTGGTGG ACAACGCTGA AGATGCGCTA ATGGGCGCCT TTGTCATCAC AGACATGATA 
AACATGATGG CCAAGTCCAC GGACCTGGAC AACGACATCG ACGAATTGAT CCAGGAGTTC 
GAGGAGCGCC GCAAGCGGGT CATCCTGCAC TCGGTGCTCT TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285455.1
CDS	86..673
Translation	MTDPSKLAVAVVCSSNMNRSMEAHNFLAKKGFNVRSYGTGERVKLPGMAFDKPNVYEFGTKYEDIYRDLESKDKEFYTQNGLLHMLDRNRRIKKCPERFQDTKEQFDIIVTVEERVYDLVVMHMESMESVDNRPVHVLNVDVVDNAEDALMGAFVITDMINMMAKSTDLDNDIDELIQEFEERRKRVILHSVLFY

Target ORF information:

RefSeq Version	NM_001298526.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ssu72 CTD phosphatase (Ssu72), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298526.1

ORF Insert Sequence:

ATGACTGATC CTAGCAAGCT CGCCGTCGCC GTAGTCTGTT CCTCGAACAT GAATCGCTCA 
ATGGAGGCGC ACAACTTTCT GGCCAAGAAG GGTTTCAATG TGCGCTCCTA CGGCACCGGC 
GAGCGCGTCA AGCTACCGGG CATGGCGTTC GACAAGCCGA ATGTTTACGA GTTTGGCACC 
AAATACGAGG ACATCTACCG CGACCTGGAG TCCAAGGACA AGGAGTTCTA CACACAGAAC 
GGCCTGCTAC ACATGCTCGA CCGCAATCGT CGCATAAAGA AGTGCCCGGA ACGGTTTCAG 
GACACCAAAG AGCAGTTCGA CATCATCGTC ACTGTGGAAG AGCGCGTCTA CGACCTGGTG 
GTGATGCACA TGGAGTCCAT GGAATCCGTG GACAACCGAC CCGTGCACGT TCTCAACGTG 
GATGTGGTGG ACAACGCTGA AGATGCGCTA ATGGGCGCCT TTGTCATCAC AGACATGATA 
AACATGATGG CCAAGTCCAC GGACCTGGAC AACGACATCG ACGAATTGAT CCAGGAGTTC 
GAGGAGCGCC GCAAGCGGGT CATCCTGCAC TCGGTGCTCT TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssu72 ( NM_134498.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608342.1 Drosophila melanogaster Ssu72 CTD phosphatase (Ssu72), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02160
Clone ID Related Accession (Same CDS sequence)	NM_001298526.1 , NM_134498.4
Accession Version	NM_134498.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ssu72 CTD phosphatase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_134498.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGATC CTAGCAAGCT CGCCGTCGCC GTAGTCTGTT CCTCGAACAT GAATCGCTCA 
ATGGAGGCGC ACAACTTTCT GGCCAAGAAG GGTTTCAATG TGCGCTCCTA CGGCACCGGC 
GAGCGCGTCA AGCTACCGGG CATGGCGTTC GACAAGCCGA ATGTTTACGA GTTTGGCACC 
AAATACGAGG ACATCTACCG CGACCTGGAG TCCAAGGACA AGGAGTTCTA CACACAGAAC 
GGCCTGCTAC ACATGCTCGA CCGCAATCGT CGCATAAAGA AGTGCCCGGA ACGGTTTCAG 
GACACCAAAG AGCAGTTCGA CATCATCGTC ACTGTGGAAG AGCGCGTCTA CGACCTGGTG 
GTGATGCACA TGGAGTCCAT GGAATCCGTG GACAACCGAC CCGTGCACGT TCTCAACGTG 
GATGTGGTGG ACAACGCTGA AGATGCGCTA ATGGGCGCCT TTGTCATCAC AGACATGATA 
AACATGATGG CCAAGTCCAC GGACCTGGAC AACGACATCG ACGAATTGAT CCAGGAGTTC 
GAGGAGCGCC GCAAGCGGGT CATCCTGCAC TCGGTGCTCT TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608342.1
CDS	86..673
Translation	MTDPSKLAVAVVCSSNMNRSMEAHNFLAKKGFNVRSYGTGERVKLPGMAFDKPNVYEFGTKYEDIYRDLESKDKEFYTQNGLLHMLDRNRRIKKCPERFQDTKEQFDIIVTVEERVYDLVVMHMESMESVDNRPVHVLNVDVVDNAEDALMGAFVITDMINMMAKSTDLDNDIDELIQEFEERRKRVILHSVLFY

Target ORF information:

RefSeq Version	NM_134498.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ssu72 CTD phosphatase (Ssu72), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134498.4

ORF Insert Sequence:

ATGACTGATC CTAGCAAGCT CGCCGTCGCC GTAGTCTGTT CCTCGAACAT GAATCGCTCA 
ATGGAGGCGC ACAACTTTCT GGCCAAGAAG GGTTTCAATG TGCGCTCCTA CGGCACCGGC 
GAGCGCGTCA AGCTACCGGG CATGGCGTTC GACAAGCCGA ATGTTTACGA GTTTGGCACC 
AAATACGAGG ACATCTACCG CGACCTGGAG TCCAAGGACA AGGAGTTCTA CACACAGAAC 
GGCCTGCTAC ACATGCTCGA CCGCAATCGT CGCATAAAGA AGTGCCCGGA ACGGTTTCAG 
GACACCAAAG AGCAGTTCGA CATCATCGTC ACTGTGGAAG AGCGCGTCTA CGACCTGGTG 
GTGATGCACA TGGAGTCCAT GGAATCCGTG GACAACCGAC CCGTGCACGT TCTCAACGTG 
GATGTGGTGG ACAACGCTGA AGATGCGCTA ATGGGCGCCT TTGTCATCAC AGACATGATA 
AACATGATGG CCAAGTCCAC GGACCTGGAC AACGACATCG ACGAATTGAT CCAGGAGTTC 
GAGGAGCGCC GCAAGCGGGT CATCCTGCAC TCGGTGCTCT TCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ssu72 ( NM_001272796.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259725.1 Drosophila melanogaster Ssu72 CTD phosphatase (Ssu72), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03170
Clone ID Related Accession (Same CDS sequence)	NM_001272796.2
Accession Version	NM_001272796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ssu72 CTD phosphatase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272796.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGATC CTAGCAAGCT CGCCGTCGCC GTAGTCTGTT CCTCGAACAT GAATCGCTCA 
ATGGAGGCGC ACAACTTTCT GGCCAAGAAG GGTTTCAATG TGCGCTCCTA CGGCACCGGC 
GAGCGCGTCA AGCTACCGGG CATGGCGTTC GACAAGCCGA ATGTTTACGA GTTTGGCACC 
AAATACGAGG ACATCTACCG CGACCTGGAG TCCAAGGACA AGGACTACAC ACAGAACGGC 
CTGCTACACA TGCTCGACCG CAATCGTCGC ATAAAGAAGT GCCCGGAACG GTTTCAGGAC 
ACCAAAGAGC AGTTCGACAT CATCGTCACT GTGGAAGAGC GCGTCTACGA CCTGGTGGTG 
ATGCACATGG AGTCCATGGA ATCCGTGGAC AACCGACCCG TGCACGTTCT CAACGTGGAT 
GTGGTGGACA ACGCTGAAGA TGCGCTAATG GGCGCCTTTG TCATCACAGA CATGATAAAC 
ATGATGGCCA AGTCCACGGA CCTGGACAAC GACATCGACG AATTGATCCA GGAGTTCGAG 
GAGCGCCGCA AGCGGGTCAT CCTGCACTCG GTGCTCTTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259725.1
CDS	86..670
Translation	MTDPSKLAVAVVCSSNMNRSMEAHNFLAKKGFNVRSYGTGERVKLPGMAFDKPNVYEFGTKYEDIYRDLESKDKDYTQNGLLHMLDRNRRIKKCPERFQDTKEQFDIIVTVEERVYDLVVMHMESMESVDNRPVHVLNVDVVDNAEDALMGAFVITDMINMMAKSTDLDNDIDELIQEFEERRKRVILHSVLFY

Target ORF information:

RefSeq Version	NM_001272796.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ssu72 CTD phosphatase (Ssu72), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272796.2

ORF Insert Sequence:

ATGACTGATC CTAGCAAGCT CGCCGTCGCC GTAGTCTGTT CCTCGAACAT GAATCGCTCA 
ATGGAGGCGC ACAACTTTCT GGCCAAGAAG GGTTTCAATG TGCGCTCCTA CGGCACCGGC 
GAGCGCGTCA AGCTACCGGG CATGGCGTTC GACAAGCCGA ATGTTTACGA GTTTGGCACC 
AAATACGAGG ACATCTACCG CGACCTGGAG TCCAAGGACA AGGACTACAC ACAGAACGGC 
CTGCTACACA TGCTCGACCG CAATCGTCGC ATAAAGAAGT GCCCGGAACG GTTTCAGGAC 
ACCAAAGAGC AGTTCGACAT CATCGTCACT GTGGAAGAGC GCGTCTACGA CCTGGTGGTG 
ATGCACATGG AGTCCATGGA ATCCGTGGAC AACCGACCCG TGCACGTTCT CAACGTGGAT 
GTGGTGGACA ACGCTGAAGA TGCGCTAATG GGCGCCTTTG TCATCACAGA CATGATAAAC 
ATGATGGCCA AGTCCACGGA CCTGGACAAC GACATCGACG AATTGATCCA GGAGTTCGAG 
GAGCGCCGCA AGCGGGTCAT CCTGCACTCG GTGCTCTTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ssu72 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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