e(y)3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	e(y)3
Entrez Gene ID	32965
Full Name	enhancer of yellow 3
Synonyms	CG12238,Dmel\CG12238,SAYP,e(y)[3],l(1)G0084,l(1)G0266,l(1)G0283,l(1)G0381,l(1)G0409,sayp
General protein information	Preferred Names enhancer of yellow 3 Names CG12238 gene product from transcript CG12238-RB CG12238-PA CG12238-PB CG12238-PC CG12238-PD CG12238-PE SAYP e(y)3-PA e(y)3-PB e(y)3-PC e(y)3-PD e(y)3-PE lethal (1) G0084 supporter of activation of yellow protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18D8-18D11\|1-62.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_134490.3	NP_608334.3	enhancer of yellow 3, isoform A
NM_001272791.1	NP_001259720.1	enhancer of yellow 3, isoform D
NM_001272790.1	NP_001259719.1	enhancer of yellow 3, isoform C
NM_001272792.1	NP_001259721.1	enhancer of yellow 3, isoform E
NM_001272789.1	NP_001259718.1	enhancer of yellow 3, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the repressed state of the ftz-f1 gene, the Pol II complex is pre-recruited on the promoter; Pol II starts transcription but is paused 1.5 kb downstream of the promoter, with SAYP and Brahma forming a nucleosomal barrier ahead of paused Pol II.
Title: SAYP and Brahma are important for 'repressive' and 'transient' Pol II pausing.

SAYP interacts with DHR3 nuclear receptor and activates the corresponding genes by recruiting the BTFly (Brahma and TFIID) coactivator supercomplex during development and have multiple common targets across the genome.
Title: [SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation].

The transcription coactivators SAYP and ENY2 act in gene expression as cooperative nanoregulators,determining the molecular mechanisms of spatial and temporal coordination of the processes involved in the realization of genetic information.
Title: [Functioning of macromolecular complexes at successive stages of gene expression as self-coordinated molecular machines].

DHR3 and SAYP interact during development and have multiple common targets across the genome
Title: SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation.

Novel conservative domain of SAYP coactivator mediates TFIID and Brahma transcriptional complexes interaction
Title: [Novel conservative domain of SAYP coactivator mediates TFIID and Brahma transcriptional complexes interaction].

The following e(y)3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the e(y)3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02152	NM_134490.3 Latest version!	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa02152	NM_001272792.1 Latest version!	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa02152	NM_001272789.1 Latest version!	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03167	NM_001272790.1 Latest version!	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03168	NM_001272791.1 Latest version!	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

e(y)3 ( NM_134490.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608334.3 Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02152
Clone ID Related Accession (Same CDS sequence)	NM_134490.3 , NM_001272792.1 , NM_001272789.1
Accession Version	NM_134490.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of yellow 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_134490.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGACTCCCT CCCAGCAAAC AGCGACGTCT GGACAACGAC 
GAGAACGACC AGCAGACGCA GAATTGCCAC GATCCCGCCA GAAAGTGCAG TGAATCACTG 
CAGGCGCTTC CGCCGTCACA TCGCTCGCGC AAGCAGAAGC ACGAGCGAAT CCTGAACACA 
GATCTGGAGG GTTCGCTTTT TCCACCCACC CAACAGCAGT CGATTTCAAC GCCGGATCAA 
AATGGAGCCT TGTCCTCGGA GGTCGAAGGC GAGGACGCAA AGGCGCAAGA TCCGCTAGAA 
CCGGCGACGC CTCAGCCCCC ACCGGTAGCG ACGCCGGCAG GAACGGGCAA GAAGCGTGGG 
CGGCCGAAGC GTATCCAAAA CCAGAGCTCG CCGGATGGTG GAGGTGCAGT TACTCCCAAA 
CCCGGCACTC CACAGGAGGA GGCCAACAGC GCTGTCAAGT CGCGTCGCGT ACAACTGCTG 
CGCAAGCGAC TGGCCATCGA CATGGTGAGC GTGGGCCAAG AGCAGGCGGA CATGAAAGCT 
AAGGAAAAGG AGTCGTCTGT GGGCGTGCCA AATGCAAGAG TTGAAGACGG GCTTGCCGAA 
ACTCTGGAAT CTCCAAAGAC TCGCGACCAT CGCCCCCTGA GAGCCACGCG CCGCACCACG 
ACCAGCACAA ACACCAACCT GCAGCCCACG CCCAAGTCCA CAAGGAAGCG CCAGAGCAAG 
GCCAGTGTCC AACAAAGCCA GTTGCCGCCG CCGACGATGG CTCAGTTCGG TAGTTCGGAG 
TCCGAGTCCA ACAACAACAA TAATAGCAGC ATATCCTTTG CCCTGAGCGC GCAGATCGAC 
CTCACAATGT GCTCGTCCAG TTCGTCGACC TCCTCTGGCG CTGCCGCCAA CCAGCAGGTG 
ATCGGTGGAA GTGGCAGCAG CTCGATGCTA CCTCCGACGA CGATTCTGTC TTCTTCGGAT 
CCGCTGCCCG ATGTCATCTT CCAGCCAAAT GACTTCTCCT CTATCATGGC CACCCAGCAG 
CTGCGCTCCT CGCGCCCCTC AAGCATCAGC TGTGGCAGTA CCGGCGGTAG CCAGCCGGAC 
TGTCACGACG AGGACAACTA CACCAGCGCG CTGGACAATT CCGGAGACGA AACTGCGTTG 
CCTATGCCCC CCACGCGGGG ACGTGGTCGT GGCCGGCGAT CCAGGGGAGG CCGCGGTAGA 
GGATCTTCTT CGGTGGATCG GGCTGTCAGC GTCGGAGGCA CAGCCTCCAC AACGCCCGCC 
ACGCAGCCTA GGGCCCCGCG AATGAGCCGA GGAGCAAGTG CCGTAGCAAA GGCCATCGCC 
ATGAGTCGAC CACGATGCGT GGGCGGCCTC AAGCACCAGC CCGATCCCGA GAGACTTAAA 
GGCTTGTTCA GTCCGTCGCC GCAAGTGTTT GAGGAGGACA CGCGTATGAG CGCCGATCTT 
AGCAACAGCA ATCAGTCGAT GGCCAGCCTG ATGGAACCCC CGCTAACGCC ACAGAAGCAA 
CCCGACTTCC TCAACAACGA GGAGTCACAG TCGTCCATGG TGAGCAATGT GAGCATGATG 
GACTCCAACC AGGGTCAGTC CGCCGACGCG ATTCTGGGTT CTGCTCTCAA ACGTCCAAAG 
AAGAAGAAGA TGGAAACTTG CGTGGCCGAA GACACCGACT ACAGCGCCTC CAGCATCGCC 
GAGTACGACT GGCCACCGCC TAAAGGTTGC TGCCCATCAA AGAATCGGGA CACCTTCATG 
ATTCAAGAGC AGGTGGCGCT CTATCTGGGC ATTACCAGCT TTAAGCGCAA GTACCCGGAT 
CTGCCACGCC GATCGGTCGA CATGGAGGAG CGCAATTGGC TGCAGGAGAA GGGGCTGGTT 
AGCGAGAAGT TGTGCGACCT GGGCATCACC GCTGTGTGGG CCTCCGACAT ATTGGACATC 
ATGTACGCGG ACTTTTTCGA CAAGTACGAA GAGTACAAGG AGTACATTCG CAGAAAGCAT 
TTGCGCGAGA TCGAGGCTAA GCAGAAGGCC CTAGGACTCA CAGTCGGGGC CGGACGAGGT 
CTGCAAGCTC GCGACCGTGC CATGCTTTCG GCCACCAAGT GGAACGCTTA CTTCAACAAG 
TCCCGTAAGG ATGAGCGCCA GTCCTGCATG GACCTGCAGA CCTTCACGAT AAATCAGCCG 
CAGCCAAGGA CCGCGCCCAC CTGTACACGC CTAGAGCGCT CCACTTCGGA TTTGATACGG 
GCAGTGGCAG AAGAGGCGAA TATCCCGGCA CCGCCTAATC TCCTGCCACC CCGCGACTAT 
GACGAGGCCT TCCGCAACTC AGACTACGCT TATCCACTCA CGGTGGTACC GGACCAGTTC 
TCAATGGCCT ACCGCCAGTT TGAGCCCGCC GAGCTCAGAG CCTACCCACT GGACACGGCG 
CTGGACAAGC CGCCGACCGA TCTGATGGCT CAGCTGCTAC AGGCAAAATC CGAAGCTGTA 
GGCAGCGATG AAATCAAAAC GTCCGCCCCG GCCCCCAAGG ATCTGGGGCA AGAGCAGTCC 
GCCATCAAAT CCGTAACGGT AACGGCGCCC GTCCGACGCA GCAGAAGGTC CACACGCCAG 
CAGACGGACA AGGTTCGCAC GGCTAGCAGC TCCAGCACGT CCTCCGCTCA ATCGGTCTCA 
TCCGCGTCCA GCGGCAACGG GAGCAGCAGT GATACGGAAA GCGGCGATGA AAGCGACTTT 
AGCAGCACAT CGAGCTGCAG CAGTTCGACG GGTGCCTCAA GTGGAGCAGG CAGCGAGGAC 
GAGGATGGGA ACGAGTGCTC ATCGTCGGTC AGGCTGTCCA CCTGCGGCGT CTGTCTGCGC 
AGTCAGCATC GCAATGCTAG GGACATGCCG GAGGCTTTCA TCCGCTGTTA CACATGCCGA 
AAGCGCGTCC ATCCCAGCTG CGTTGATATG CCACCGCGCA TGGTGGGTCG CGTGAGGAAC 
TACAATTGGC AGTGCGCCGG GTGCAAATGC TGCATCAAGT GCCGTAGCAG CCAACGTCCA 
GGAAAGATGC TCTATTGCGA GCAGTGCGAC CGAGGCTACC ACATCTACTG CTTGGGCCTC 
AGGACAGTCC CAGATGGGCG CTGGAGCTGT GAGCGCTGCT GTTTCTGCAT GCGATGCGGG 
GCCACCAAGC CGGAGGGTCT GCCCCAGGTG GCCGCTCTGT CCCAGGCGTC CGGAGGACCG 
TCGGCCAACG GGGATCGCTC AAAGGCAGCG CGCAATAAGC GCTTGAAATG GGTGCACGAG 
TACCGCATCG ATCATGTGAC GAAGATCCGG GAGCACGCCG CCATGTTCTG CGTACCATGT 
GCGCGAAACA AGCCCGCCAA GCGGCAGTCG GCGGCGGGTG CGGCCGGTGC GGCAGCAGTT 
ACTCCTGTCT TGGAGGCCAC TTCCGCGCAA ACGGATGACA GTCCGATGCC CAGTCCCGGC 
CTGACAACCA ACGGAGGACG CGCCCTTTCG CCAACGGCGG CACTGAGTCC AAAGGCAGCT 
GTGCCCGTGG CATCGCTGCC GCCCGTTCTT GAAGCGACGA CGGTGACCAC AAATATTGCG 
GGAACGATAG GCAGACGGCA GGCGGGTAAT GCTGTCAATA TTACAACGAT GCAGTGCAGC 
AGCAGCAGCA GCAGCAACTT CAGCGGGAAC GGAGTCACCG AGGACGCGGC GAATGTCACT 
GCAACGGGAA CGGCTACGGC GGCCGCAGGA GCACCCGCTG CCACTCCAAT AGGCATAGCT 
CCGCCGCCCG TTGTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608334.3
CDS	785..6805
Translation	MNDLRQQQMVAATSSSGSESGTAVESAAATSTAGSAGAAGRPQSNCSANSNAKSVAASSTSEEEQRVSSTSSPAQRDQQLNADRDREQEPEPQQQQQREEALQHQHNQPGHITSTTASPPPTLPPPTTPCDDAPSTTGASASASSASGEAPSAASAAGAAGGPMAATALEVESEERDGHKIILKLSKHANPNSNANESQPGGDERRVEPLRIQLPCGGAEGGLVAKQQDAFDADASSCSSSCAEDEVATTLGQQLRNTPHIVPKLTIRAANERRVGSVVPKLTIKLPENPAASGSNSNSGSCSAAVSGAQSAMPAKNDAHLSSLSPASASSSSASSSSSSSSSSSLAEMQTVPKLMIKTTLAGSSCISSSEELPQQQQQIPKLTIKTGGGGQEHVHTVIMTHDLNNAQSIPKLTIKTKSIEMIEDEQAAKLEQQLQPLPKLTIKNLCSPKHKVRAVLEEKPPTAASKLAIPKPTPNPTPAPMTNGGESNSSSQEFCGFSDPDADIAAPASDDVRRNSDDMVIDDSLSKEHDPKIFHNLPPMPASNGIASGGSKASKASKSAQSQHNVVDMVDLTSSPSPGSSPTHVPNNFTGRISPKGLLIDCLRMQTAIGCYVPEEADSRVRPAASPNSNILLSQLTAPAKSFTTTTPKSKSSKYPQLTERLMANGSGTGGGSVIAAESEAVVGPLPAANPAQSIIESIEILDTPDGSPRVAYMDEDSNPMLNKHLQTIHKLAMDHGVEQRMDTQDNNNENHLKRTNSEGNESPSSRLPPSKQRRLDNDENDQQTQNCHDPARKCSESLQALPPSHRSRKQKHERILNTDLEGSLFPPTQQQSISTPDQNGALSSEVEGEDAKAQDPLEPATPQPPPVATPAGTGKKRGRPKRIQNQSSPDGGGAVTPKPGTPQEEANSAVKSRRVQLLRKRLAIDMVSVGQEQADMKAKEKESSVGVPNARVEDGLAETLESPKTRDHRPLRATRRTTTSTNTNLQPTPKSTRKRQSKASVQQSQLPPPTMAQFGSSESESNNNNNSSISFALSAQIDLTMCSSSSSTSSGAAANQQVIGGSGSSSMLPPTTILSSSDPLPDVIFQPNDFSSIMATQQLRSSRPSSISCGSTGGSQPDCHDEDNYTSALDNSGDETALPMPPTRGRGRGRRSRGGRGRGSSSVDRAVSVGGTASTTPATQPRAPRMSRGASAVAKAIAMSRPRCVGGLKHQPDPERLKGLFSPSPQVFEEDTRMSADLSNSNQSMASLMEPPLTPQKQPDFLNNEESQSSMVSNVSMMDSNQGQSADAILGSALKRPKKKKMETCVAEDTDYSASSIAEYDWPPPKGCCPSKNRDTFMIQEQVALYLGITSFKRKYPDLPRRSVDMEERNWLQEKGLVSEKLCDLGITAVWASDILDIMYADFFDKYEEYKEYIRRKHLREIEAKQKALGLTVGAGRGLQARDRAMLSATKWNAYFNKSRKDERQSCMDLQTFTINQPQPRTAPTCTRLERSTSDLIRAVAEEANIPAPPNLLPPRDYDEAFRNSDYAYPLTVVPDQFSMAYRQFEPAELRAYPLDTALDKPPTDLMAQLLQAKSEAVGSDEIKTSAPAPKDLGQEQSAIKSVTVTAPVRRSRRSTRQQTDKVRTASSSSTSSAQSVSSASSGNGSSSDTESGDESDFSSTSSCSSSTGASSGAGSEDEDGNECSSSVRLSTCGVCLRSQHRNARDMPEAFIRCYTCRKRVHPSCVDMPPRMVGRVRNYNWQCAGCKCCIKCRSSQRPGKMLYCEQCDRGYHIYCLGLRTVPDGRWSCERCCFCMRCGATKPEGLPQVAALSQASGGPSANGDRSKAARNKRLKWVHEYRIDHVTKIREHAAMFCVPCARNKPAKRQSAAGAAGAAAVTPVLEATSAQTDDSPMPSPGLTTNGGRALSPTAALSPKAAVPVASLPPVLEATTVTTNIAGTIGRRQAGNAVNITTMQCSSSSSSNFSGNGVTEDAANVTATGTATAAAGAPAATPIGIAPPPVVA

Target ORF information:

RefSeq Version	NM_134490.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134490.3

ORF Insert Sequence:

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGACTCCCT CCCAGCAAAC AGCGACGTCT GGACAACGAC 
GAGAACGACC AGCAGACGCA GAATTGCCAC GATCCCGCCA GAAAGTGCAG TGAATCACTG 
CAGGCGCTTC CGCCGTCACA TCGCTCGCGC AAGCAGAAGC ACGAGCGAAT CCTGAACACA 
GATCTGGAGG GTTCGCTTTT TCCACCCACC CAACAGCAGT CGATTTCAAC GCCGGATCAA 
AATGGAGCCT TGTCCTCGGA GGTCGAAGGC GAGGACGCAA AGGCGCAAGA TCCGCTAGAA 
CCGGCGACGC CTCAGCCCCC ACCGGTAGCG ACGCCGGCAG GAACGGGCAA GAAGCGTGGG 
CGGCCGAAGC GTATCCAAAA CCAGAGCTCG CCGGATGGTG GAGGTGCAGT TACTCCCAAA 
CCCGGCACTC CACAGGAGGA GGCCAACAGC GCTGTCAAGT CGCGTCGCGT ACAACTGCTG 
CGCAAGCGAC TGGCCATCGA CATGGTGAGC GTGGGCCAAG AGCAGGCGGA CATGAAAGCT 
AAGGAAAAGG AGTCGTCTGT GGGCGTGCCA AATGCAAGAG TTGAAGACGG GCTTGCCGAA 
ACTCTGGAAT CTCCAAAGAC TCGCGACCAT CGCCCCCTGA GAGCCACGCG CCGCACCACG 
ACCAGCACAA ACACCAACCT GCAGCCCACG CCCAAGTCCA CAAGGAAGCG CCAGAGCAAG 
GCCAGTGTCC AACAAAGCCA GTTGCCGCCG CCGACGATGG CTCAGTTCGG TAGTTCGGAG 
TCCGAGTCCA ACAACAACAA TAATAGCAGC ATATCCTTTG CCCTGAGCGC GCAGATCGAC 
CTCACAATGT GCTCGTCCAG TTCGTCGACC TCCTCTGGCG CTGCCGCCAA CCAGCAGGTG 
ATCGGTGGAA GTGGCAGCAG CTCGATGCTA CCTCCGACGA CGATTCTGTC TTCTTCGGAT 
CCGCTGCCCG ATGTCATCTT CCAGCCAAAT GACTTCTCCT CTATCATGGC CACCCAGCAG 
CTGCGCTCCT CGCGCCCCTC AAGCATCAGC TGTGGCAGTA CCGGCGGTAG CCAGCCGGAC 
TGTCACGACG AGGACAACTA CACCAGCGCG CTGGACAATT CCGGAGACGA AACTGCGTTG 
CCTATGCCCC CCACGCGGGG ACGTGGTCGT GGCCGGCGAT CCAGGGGAGG CCGCGGTAGA 
GGATCTTCTT CGGTGGATCG GGCTGTCAGC GTCGGAGGCA CAGCCTCCAC AACGCCCGCC 
ACGCAGCCTA GGGCCCCGCG AATGAGCCGA GGAGCAAGTG CCGTAGCAAA GGCCATCGCC 
ATGAGTCGAC CACGATGCGT GGGCGGCCTC AAGCACCAGC CCGATCCCGA GAGACTTAAA 
GGCTTGTTCA GTCCGTCGCC GCAAGTGTTT GAGGAGGACA CGCGTATGAG CGCCGATCTT 
AGCAACAGCA ATCAGTCGAT GGCCAGCCTG ATGGAACCCC CGCTAACGCC ACAGAAGCAA 
CCCGACTTCC TCAACAACGA GGAGTCACAG TCGTCCATGG TGAGCAATGT GAGCATGATG 
GACTCCAACC AGGGTCAGTC CGCCGACGCG ATTCTGGGTT CTGCTCTCAA ACGTCCAAAG 
AAGAAGAAGA TGGAAACTTG CGTGGCCGAA GACACCGACT ACAGCGCCTC CAGCATCGCC 
GAGTACGACT GGCCACCGCC TAAAGGTTGC TGCCCATCAA AGAATCGGGA CACCTTCATG 
ATTCAAGAGC AGGTGGCGCT CTATCTGGGC ATTACCAGCT TTAAGCGCAA GTACCCGGAT 
CTGCCACGCC GATCGGTCGA CATGGAGGAG CGCAATTGGC TGCAGGAGAA GGGGCTGGTT 
AGCGAGAAGT TGTGCGACCT GGGCATCACC GCTGTGTGGG CCTCCGACAT ATTGGACATC 
ATGTACGCGG ACTTTTTCGA CAAGTACGAA GAGTACAAGG AGTACATTCG CAGAAAGCAT 
TTGCGCGAGA TCGAGGCTAA GCAGAAGGCC CTAGGACTCA CAGTCGGGGC CGGACGAGGT 
CTGCAAGCTC GCGACCGTGC CATGCTTTCG GCCACCAAGT GGAACGCTTA CTTCAACAAG 
TCCCGTAAGG ATGAGCGCCA GTCCTGCATG GACCTGCAGA CCTTCACGAT AAATCAGCCG 
CAGCCAAGGA CCGCGCCCAC CTGTACACGC CTAGAGCGCT CCACTTCGGA TTTGATACGG 
GCAGTGGCAG AAGAGGCGAA TATCCCGGCA CCGCCTAATC TCCTGCCACC CCGCGACTAT 
GACGAGGCCT TCCGCAACTC AGACTACGCT TATCCACTCA CGGTGGTACC GGACCAGTTC 
TCAATGGCCT ACCGCCAGTT TGAGCCCGCC GAGCTCAGAG CCTACCCACT GGACACGGCG 
CTGGACAAGC CGCCGACCGA TCTGATGGCT CAGCTGCTAC AGGCAAAATC CGAAGCTGTA 
GGCAGCGATG AAATCAAAAC GTCCGCCCCG GCCCCCAAGG ATCTGGGGCA AGAGCAGTCC 
GCCATCAAAT CCGTAACGGT AACGGCGCCC GTCCGACGCA GCAGAAGGTC CACACGCCAG 
CAGACGGACA AGGTTCGCAC GGCTAGCAGC TCCAGCACGT CCTCCGCTCA ATCGGTCTCA 
TCCGCGTCCA GCGGCAACGG GAGCAGCAGT GATACGGAAA GCGGCGATGA AAGCGACTTT 
AGCAGCACAT CGAGCTGCAG CAGTTCGACG GGTGCCTCAA GTGGAGCAGG CAGCGAGGAC 
GAGGATGGGA ACGAGTGCTC ATCGTCGGTC AGGCTGTCCA CCTGCGGCGT CTGTCTGCGC 
AGTCAGCATC GCAATGCTAG GGACATGCCG GAGGCTTTCA TCCGCTGTTA CACATGCCGA 
AAGCGCGTCC ATCCCAGCTG CGTTGATATG CCACCGCGCA TGGTGGGTCG CGTGAGGAAC 
TACAATTGGC AGTGCGCCGG GTGCAAATGC TGCATCAAGT GCCGTAGCAG CCAACGTCCA 
GGAAAGATGC TCTATTGCGA GCAGTGCGAC CGAGGCTACC ACATCTACTG CTTGGGCCTC 
AGGACAGTCC CAGATGGGCG CTGGAGCTGT GAGCGCTGCT GTTTCTGCAT GCGATGCGGG 
GCCACCAAGC CGGAGGGTCT GCCCCAGGTG GCCGCTCTGT CCCAGGCGTC CGGAGGACCG 
TCGGCCAACG GGGATCGCTC AAAGGCAGCG CGCAATAAGC GCTTGAAATG GGTGCACGAG 
TACCGCATCG ATCATGTGAC GAAGATCCGG GAGCACGCCG CCATGTTCTG CGTACCATGT 
GCGCGAAACA AGCCCGCCAA GCGGCAGTCG GCGGCGGGTG CGGCCGGTGC GGCAGCAGTT 
ACTCCTGTCT TGGAGGCCAC TTCCGCGCAA ACGGATGACA GTCCGATGCC CAGTCCCGGC 
CTGACAACCA ACGGAGGACG CGCCCTTTCG CCAACGGCGG CACTGAGTCC AAAGGCAGCT 
GTGCCCGTGG CATCGCTGCC GCCCGTTCTT GAAGCGACGA CGGTGACCAC AAATATTGCG 
GGAACGATAG GCAGACGGCA GGCGGGTAAT GCTGTCAATA TTACAACGAT GCAGTGCAGC 
AGCAGCAGCA GCAGCAACTT CAGCGGGAAC GGAGTCACCG AGGACGCGGC GAATGTCACT 
GCAACGGGAA CGGCTACGGC GGCCGCAGGA GCACCCGCTG CCACTCCAAT AGGCATAGCT 
CCGCCGCCCG TTGTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

e(y)3 ( NM_001272792.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259721.1 Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant E, mRNA.

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CloneID	OFa02152
Clone ID Related Accession (Same CDS sequence)	NM_134490.3 , NM_001272792.1 , NM_001272789.1
Accession Version	NM_001272792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of yellow 3, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGACTCCCT CCCAGCAAAC AGCGACGTCT GGACAACGAC 
GAGAACGACC AGCAGACGCA GAATTGCCAC GATCCCGCCA GAAAGTGCAG TGAATCACTG 
CAGGCGCTTC CGCCGTCACA TCGCTCGCGC AAGCAGAAGC ACGAGCGAAT CCTGAACACA 
GATCTGGAGG GTTCGCTTTT TCCACCCACC CAACAGCAGT CGATTTCAAC GCCGGATCAA 
AATGGAGCCT TGTCCTCGGA GGTCGAAGGC GAGGACGCAA AGGCGCAAGA TCCGCTAGAA 
CCGGCGACGC CTCAGCCCCC ACCGGTAGCG ACGCCGGCAG GAACGGGCAA GAAGCGTGGG 
CGGCCGAAGC GTATCCAAAA CCAGAGCTCG CCGGATGGTG GAGGTGCAGT TACTCCCAAA 
CCCGGCACTC CACAGGAGGA GGCCAACAGC GCTGTCAAGT CGCGTCGCGT ACAACTGCTG 
CGCAAGCGAC TGGCCATCGA CATGGTGAGC GTGGGCCAAG AGCAGGCGGA CATGAAAGCT 
AAGGAAAAGG AGTCGTCTGT GGGCGTGCCA AATGCAAGAG TTGAAGACGG GCTTGCCGAA 
ACTCTGGAAT CTCCAAAGAC TCGCGACCAT CGCCCCCTGA GAGCCACGCG CCGCACCACG 
ACCAGCACAA ACACCAACCT GCAGCCCACG CCCAAGTCCA CAAGGAAGCG CCAGAGCAAG 
GCCAGTGTCC AACAAAGCCA GTTGCCGCCG CCGACGATGG CTCAGTTCGG TAGTTCGGAG 
TCCGAGTCCA ACAACAACAA TAATAGCAGC ATATCCTTTG CCCTGAGCGC GCAGATCGAC 
CTCACAATGT GCTCGTCCAG TTCGTCGACC TCCTCTGGCG CTGCCGCCAA CCAGCAGGTG 
ATCGGTGGAA GTGGCAGCAG CTCGATGCTA CCTCCGACGA CGATTCTGTC TTCTTCGGAT 
CCGCTGCCCG ATGTCATCTT CCAGCCAAAT GACTTCTCCT CTATCATGGC CACCCAGCAG 
CTGCGCTCCT CGCGCCCCTC AAGCATCAGC TGTGGCAGTA CCGGCGGTAG CCAGCCGGAC 
TGTCACGACG AGGACAACTA CACCAGCGCG CTGGACAATT CCGGAGACGA AACTGCGTTG 
CCTATGCCCC CCACGCGGGG ACGTGGTCGT GGCCGGCGAT CCAGGGGAGG CCGCGGTAGA 
GGATCTTCTT CGGTGGATCG GGCTGTCAGC GTCGGAGGCA CAGCCTCCAC AACGCCCGCC 
ACGCAGCCTA GGGCCCCGCG AATGAGCCGA GGAGCAAGTG CCGTAGCAAA GGCCATCGCC 
ATGAGTCGAC CACGATGCGT GGGCGGCCTC AAGCACCAGC CCGATCCCGA GAGACTTAAA 
GGCTTGTTCA GTCCGTCGCC GCAAGTGTTT GAGGAGGACA CGCGTATGAG CGCCGATCTT 
AGCAACAGCA ATCAGTCGAT GGCCAGCCTG ATGGAACCCC CGCTAACGCC ACAGAAGCAA 
CCCGACTTCC TCAACAACGA GGAGTCACAG TCGTCCATGG TGAGCAATGT GAGCATGATG 
GACTCCAACC AGGGTCAGTC CGCCGACGCG ATTCTGGGTT CTGCTCTCAA ACGTCCAAAG 
AAGAAGAAGA TGGAAACTTG CGTGGCCGAA GACACCGACT ACAGCGCCTC CAGCATCGCC 
GAGTACGACT GGCCACCGCC TAAAGGTTGC TGCCCATCAA AGAATCGGGA CACCTTCATG 
ATTCAAGAGC AGGTGGCGCT CTATCTGGGC ATTACCAGCT TTAAGCGCAA GTACCCGGAT 
CTGCCACGCC GATCGGTCGA CATGGAGGAG CGCAATTGGC TGCAGGAGAA GGGGCTGGTT 
AGCGAGAAGT TGTGCGACCT GGGCATCACC GCTGTGTGGG CCTCCGACAT ATTGGACATC 
ATGTACGCGG ACTTTTTCGA CAAGTACGAA GAGTACAAGG AGTACATTCG CAGAAAGCAT 
TTGCGCGAGA TCGAGGCTAA GCAGAAGGCC CTAGGACTCA CAGTCGGGGC CGGACGAGGT 
CTGCAAGCTC GCGACCGTGC CATGCTTTCG GCCACCAAGT GGAACGCTTA CTTCAACAAG 
TCCCGTAAGG ATGAGCGCCA GTCCTGCATG GACCTGCAGA CCTTCACGAT AAATCAGCCG 
CAGCCAAGGA CCGCGCCCAC CTGTACACGC CTAGAGCGCT CCACTTCGGA TTTGATACGG 
GCAGTGGCAG AAGAGGCGAA TATCCCGGCA CCGCCTAATC TCCTGCCACC CCGCGACTAT 
GACGAGGCCT TCCGCAACTC AGACTACGCT TATCCACTCA CGGTGGTACC GGACCAGTTC 
TCAATGGCCT ACCGCCAGTT TGAGCCCGCC GAGCTCAGAG CCTACCCACT GGACACGGCG 
CTGGACAAGC CGCCGACCGA TCTGATGGCT CAGCTGCTAC AGGCAAAATC CGAAGCTGTA 
GGCAGCGATG AAATCAAAAC GTCCGCCCCG GCCCCCAAGG ATCTGGGGCA AGAGCAGTCC 
GCCATCAAAT CCGTAACGGT AACGGCGCCC GTCCGACGCA GCAGAAGGTC CACACGCCAG 
CAGACGGACA AGGTTCGCAC GGCTAGCAGC TCCAGCACGT CCTCCGCTCA ATCGGTCTCA 
TCCGCGTCCA GCGGCAACGG GAGCAGCAGT GATACGGAAA GCGGCGATGA AAGCGACTTT 
AGCAGCACAT CGAGCTGCAG CAGTTCGACG GGTGCCTCAA GTGGAGCAGG CAGCGAGGAC 
GAGGATGGGA ACGAGTGCTC ATCGTCGGTC AGGCTGTCCA CCTGCGGCGT CTGTCTGCGC 
AGTCAGCATC GCAATGCTAG GGACATGCCG GAGGCTTTCA TCCGCTGTTA CACATGCCGA 
AAGCGCGTCC ATCCCAGCTG CGTTGATATG CCACCGCGCA TGGTGGGTCG CGTGAGGAAC 
TACAATTGGC AGTGCGCCGG GTGCAAATGC TGCATCAAGT GCCGTAGCAG CCAACGTCCA 
GGAAAGATGC TCTATTGCGA GCAGTGCGAC CGAGGCTACC ACATCTACTG CTTGGGCCTC 
AGGACAGTCC CAGATGGGCG CTGGAGCTGT GAGCGCTGCT GTTTCTGCAT GCGATGCGGG 
GCCACCAAGC CGGAGGGTCT GCCCCAGGTG GCCGCTCTGT CCCAGGCGTC CGGAGGACCG 
TCGGCCAACG GGGATCGCTC AAAGGCAGCG CGCAATAAGC GCTTGAAATG GGTGCACGAG 
TACCGCATCG ATCATGTGAC GAAGATCCGG GAGCACGCCG CCATGTTCTG CGTACCATGT 
GCGCGAAACA AGCCCGCCAA GCGGCAGTCG GCGGCGGGTG CGGCCGGTGC GGCAGCAGTT 
ACTCCTGTCT TGGAGGCCAC TTCCGCGCAA ACGGATGACA GTCCGATGCC CAGTCCCGGC 
CTGACAACCA ACGGAGGACG CGCCCTTTCG CCAACGGCGG CACTGAGTCC AAAGGCAGCT 
GTGCCCGTGG CATCGCTGCC GCCCGTTCTT GAAGCGACGA CGGTGACCAC AAATATTGCG 
GGAACGATAG GCAGACGGCA GGCGGGTAAT GCTGTCAATA TTACAACGAT GCAGTGCAGC 
AGCAGCAGCA GCAGCAACTT CAGCGGGAAC GGAGTCACCG AGGACGCGGC GAATGTCACT 
GCAACGGGAA CGGCTACGGC GGCCGCAGGA GCACCCGCTG CCACTCCAAT AGGCATAGCT 
CCGCCGCCCG TTGTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259721.1
CDS	379..6399
Translation	MNDLRQQQMVAATSSSGSESGTAVESAAATSTAGSAGAAGRPQSNCSANSNAKSVAASSTSEEEQRVSSTSSPAQRDQQLNADRDREQEPEPQQQQQREEALQHQHNQPGHITSTTASPPPTLPPPTTPCDDAPSTTGASASASSASGEAPSAASAAGAAGGPMAATALEVESEERDGHKIILKLSKHANPNSNANESQPGGDERRVEPLRIQLPCGGAEGGLVAKQQDAFDADASSCSSSCAEDEVATTLGQQLRNTPHIVPKLTIRAANERRVGSVVPKLTIKLPENPAASGSNSNSGSCSAAVSGAQSAMPAKNDAHLSSLSPASASSSSASSSSSSSSSSSLAEMQTVPKLMIKTTLAGSSCISSSEELPQQQQQIPKLTIKTGGGGQEHVHTVIMTHDLNNAQSIPKLTIKTKSIEMIEDEQAAKLEQQLQPLPKLTIKNLCSPKHKVRAVLEEKPPTAASKLAIPKPTPNPTPAPMTNGGESNSSSQEFCGFSDPDADIAAPASDDVRRNSDDMVIDDSLSKEHDPKIFHNLPPMPASNGIASGGSKASKASKSAQSQHNVVDMVDLTSSPSPGSSPTHVPNNFTGRISPKGLLIDCLRMQTAIGCYVPEEADSRVRPAASPNSNILLSQLTAPAKSFTTTTPKSKSSKYPQLTERLMANGSGTGGGSVIAAESEAVVGPLPAANPAQSIIESIEILDTPDGSPRVAYMDEDSNPMLNKHLQTIHKLAMDHGVEQRMDTQDNNNENHLKRTNSEGNESPSSRLPPSKQRRLDNDENDQQTQNCHDPARKCSESLQALPPSHRSRKQKHERILNTDLEGSLFPPTQQQSISTPDQNGALSSEVEGEDAKAQDPLEPATPQPPPVATPAGTGKKRGRPKRIQNQSSPDGGGAVTPKPGTPQEEANSAVKSRRVQLLRKRLAIDMVSVGQEQADMKAKEKESSVGVPNARVEDGLAETLESPKTRDHRPLRATRRTTTSTNTNLQPTPKSTRKRQSKASVQQSQLPPPTMAQFGSSESESNNNNNSSISFALSAQIDLTMCSSSSSTSSGAAANQQVIGGSGSSSMLPPTTILSSSDPLPDVIFQPNDFSSIMATQQLRSSRPSSISCGSTGGSQPDCHDEDNYTSALDNSGDETALPMPPTRGRGRGRRSRGGRGRGSSSVDRAVSVGGTASTTPATQPRAPRMSRGASAVAKAIAMSRPRCVGGLKHQPDPERLKGLFSPSPQVFEEDTRMSADLSNSNQSMASLMEPPLTPQKQPDFLNNEESQSSMVSNVSMMDSNQGQSADAILGSALKRPKKKKMETCVAEDTDYSASSIAEYDWPPPKGCCPSKNRDTFMIQEQVALYLGITSFKRKYPDLPRRSVDMEERNWLQEKGLVSEKLCDLGITAVWASDILDIMYADFFDKYEEYKEYIRRKHLREIEAKQKALGLTVGAGRGLQARDRAMLSATKWNAYFNKSRKDERQSCMDLQTFTINQPQPRTAPTCTRLERSTSDLIRAVAEEANIPAPPNLLPPRDYDEAFRNSDYAYPLTVVPDQFSMAYRQFEPAELRAYPLDTALDKPPTDLMAQLLQAKSEAVGSDEIKTSAPAPKDLGQEQSAIKSVTVTAPVRRSRRSTRQQTDKVRTASSSSTSSAQSVSSASSGNGSSSDTESGDESDFSSTSSCSSSTGASSGAGSEDEDGNECSSSVRLSTCGVCLRSQHRNARDMPEAFIRCYTCRKRVHPSCVDMPPRMVGRVRNYNWQCAGCKCCIKCRSSQRPGKMLYCEQCDRGYHIYCLGLRTVPDGRWSCERCCFCMRCGATKPEGLPQVAALSQASGGPSANGDRSKAARNKRLKWVHEYRIDHVTKIREHAAMFCVPCARNKPAKRQSAAGAAGAAAVTPVLEATSAQTDDSPMPSPGLTTNGGRALSPTAALSPKAAVPVASLPPVLEATTVTTNIAGTIGRRQAGNAVNITTMQCSSSSSSNFSGNGVTEDAANVTATGTATAAAGAPAATPIGIAPPPVVA

Target ORF information:

RefSeq Version	NM_001272792.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272792.1

ORF Insert Sequence:

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGACTCCCT CCCAGCAAAC AGCGACGTCT GGACAACGAC 
GAGAACGACC AGCAGACGCA GAATTGCCAC GATCCCGCCA GAAAGTGCAG TGAATCACTG 
CAGGCGCTTC CGCCGTCACA TCGCTCGCGC AAGCAGAAGC ACGAGCGAAT CCTGAACACA 
GATCTGGAGG GTTCGCTTTT TCCACCCACC CAACAGCAGT CGATTTCAAC GCCGGATCAA 
AATGGAGCCT TGTCCTCGGA GGTCGAAGGC GAGGACGCAA AGGCGCAAGA TCCGCTAGAA 
CCGGCGACGC CTCAGCCCCC ACCGGTAGCG ACGCCGGCAG GAACGGGCAA GAAGCGTGGG 
CGGCCGAAGC GTATCCAAAA CCAGAGCTCG CCGGATGGTG GAGGTGCAGT TACTCCCAAA 
CCCGGCACTC CACAGGAGGA GGCCAACAGC GCTGTCAAGT CGCGTCGCGT ACAACTGCTG 
CGCAAGCGAC TGGCCATCGA CATGGTGAGC GTGGGCCAAG AGCAGGCGGA CATGAAAGCT 
AAGGAAAAGG AGTCGTCTGT GGGCGTGCCA AATGCAAGAG TTGAAGACGG GCTTGCCGAA 
ACTCTGGAAT CTCCAAAGAC TCGCGACCAT CGCCCCCTGA GAGCCACGCG CCGCACCACG 
ACCAGCACAA ACACCAACCT GCAGCCCACG CCCAAGTCCA CAAGGAAGCG CCAGAGCAAG 
GCCAGTGTCC AACAAAGCCA GTTGCCGCCG CCGACGATGG CTCAGTTCGG TAGTTCGGAG 
TCCGAGTCCA ACAACAACAA TAATAGCAGC ATATCCTTTG CCCTGAGCGC GCAGATCGAC 
CTCACAATGT GCTCGTCCAG TTCGTCGACC TCCTCTGGCG CTGCCGCCAA CCAGCAGGTG 
ATCGGTGGAA GTGGCAGCAG CTCGATGCTA CCTCCGACGA CGATTCTGTC TTCTTCGGAT 
CCGCTGCCCG ATGTCATCTT CCAGCCAAAT GACTTCTCCT CTATCATGGC CACCCAGCAG 
CTGCGCTCCT CGCGCCCCTC AAGCATCAGC TGTGGCAGTA CCGGCGGTAG CCAGCCGGAC 
TGTCACGACG AGGACAACTA CACCAGCGCG CTGGACAATT CCGGAGACGA AACTGCGTTG 
CCTATGCCCC CCACGCGGGG ACGTGGTCGT GGCCGGCGAT CCAGGGGAGG CCGCGGTAGA 
GGATCTTCTT CGGTGGATCG GGCTGTCAGC GTCGGAGGCA CAGCCTCCAC AACGCCCGCC 
ACGCAGCCTA GGGCCCCGCG AATGAGCCGA GGAGCAAGTG CCGTAGCAAA GGCCATCGCC 
ATGAGTCGAC CACGATGCGT GGGCGGCCTC AAGCACCAGC CCGATCCCGA GAGACTTAAA 
GGCTTGTTCA GTCCGTCGCC GCAAGTGTTT GAGGAGGACA CGCGTATGAG CGCCGATCTT 
AGCAACAGCA ATCAGTCGAT GGCCAGCCTG ATGGAACCCC CGCTAACGCC ACAGAAGCAA 
CCCGACTTCC TCAACAACGA GGAGTCACAG TCGTCCATGG TGAGCAATGT GAGCATGATG 
GACTCCAACC AGGGTCAGTC CGCCGACGCG ATTCTGGGTT CTGCTCTCAA ACGTCCAAAG 
AAGAAGAAGA TGGAAACTTG CGTGGCCGAA GACACCGACT ACAGCGCCTC CAGCATCGCC 
GAGTACGACT GGCCACCGCC TAAAGGTTGC TGCCCATCAA AGAATCGGGA CACCTTCATG 
ATTCAAGAGC AGGTGGCGCT CTATCTGGGC ATTACCAGCT TTAAGCGCAA GTACCCGGAT 
CTGCCACGCC GATCGGTCGA CATGGAGGAG CGCAATTGGC TGCAGGAGAA GGGGCTGGTT 
AGCGAGAAGT TGTGCGACCT GGGCATCACC GCTGTGTGGG CCTCCGACAT ATTGGACATC 
ATGTACGCGG ACTTTTTCGA CAAGTACGAA GAGTACAAGG AGTACATTCG CAGAAAGCAT 
TTGCGCGAGA TCGAGGCTAA GCAGAAGGCC CTAGGACTCA CAGTCGGGGC CGGACGAGGT 
CTGCAAGCTC GCGACCGTGC CATGCTTTCG GCCACCAAGT GGAACGCTTA CTTCAACAAG 
TCCCGTAAGG ATGAGCGCCA GTCCTGCATG GACCTGCAGA CCTTCACGAT AAATCAGCCG 
CAGCCAAGGA CCGCGCCCAC CTGTACACGC CTAGAGCGCT CCACTTCGGA TTTGATACGG 
GCAGTGGCAG AAGAGGCGAA TATCCCGGCA CCGCCTAATC TCCTGCCACC CCGCGACTAT 
GACGAGGCCT TCCGCAACTC AGACTACGCT TATCCACTCA CGGTGGTACC GGACCAGTTC 
TCAATGGCCT ACCGCCAGTT TGAGCCCGCC GAGCTCAGAG CCTACCCACT GGACACGGCG 
CTGGACAAGC CGCCGACCGA TCTGATGGCT CAGCTGCTAC AGGCAAAATC CGAAGCTGTA 
GGCAGCGATG AAATCAAAAC GTCCGCCCCG GCCCCCAAGG ATCTGGGGCA AGAGCAGTCC 
GCCATCAAAT CCGTAACGGT AACGGCGCCC GTCCGACGCA GCAGAAGGTC CACACGCCAG 
CAGACGGACA AGGTTCGCAC GGCTAGCAGC TCCAGCACGT CCTCCGCTCA ATCGGTCTCA 
TCCGCGTCCA GCGGCAACGG GAGCAGCAGT GATACGGAAA GCGGCGATGA AAGCGACTTT 
AGCAGCACAT CGAGCTGCAG CAGTTCGACG GGTGCCTCAA GTGGAGCAGG CAGCGAGGAC 
GAGGATGGGA ACGAGTGCTC ATCGTCGGTC AGGCTGTCCA CCTGCGGCGT CTGTCTGCGC 
AGTCAGCATC GCAATGCTAG GGACATGCCG GAGGCTTTCA TCCGCTGTTA CACATGCCGA 
AAGCGCGTCC ATCCCAGCTG CGTTGATATG CCACCGCGCA TGGTGGGTCG CGTGAGGAAC 
TACAATTGGC AGTGCGCCGG GTGCAAATGC TGCATCAAGT GCCGTAGCAG CCAACGTCCA 
GGAAAGATGC TCTATTGCGA GCAGTGCGAC CGAGGCTACC ACATCTACTG CTTGGGCCTC 
AGGACAGTCC CAGATGGGCG CTGGAGCTGT GAGCGCTGCT GTTTCTGCAT GCGATGCGGG 
GCCACCAAGC CGGAGGGTCT GCCCCAGGTG GCCGCTCTGT CCCAGGCGTC CGGAGGACCG 
TCGGCCAACG GGGATCGCTC AAAGGCAGCG CGCAATAAGC GCTTGAAATG GGTGCACGAG 
TACCGCATCG ATCATGTGAC GAAGATCCGG GAGCACGCCG CCATGTTCTG CGTACCATGT 
GCGCGAAACA AGCCCGCCAA GCGGCAGTCG GCGGCGGGTG CGGCCGGTGC GGCAGCAGTT 
ACTCCTGTCT TGGAGGCCAC TTCCGCGCAA ACGGATGACA GTCCGATGCC CAGTCCCGGC 
CTGACAACCA ACGGAGGACG CGCCCTTTCG CCAACGGCGG CACTGAGTCC AAAGGCAGCT 
GTGCCCGTGG CATCGCTGCC GCCCGTTCTT GAAGCGACGA CGGTGACCAC AAATATTGCG 
GGAACGATAG GCAGACGGCA GGCGGGTAAT GCTGTCAATA TTACAACGAT GCAGTGCAGC 
AGCAGCAGCA GCAGCAACTT CAGCGGGAAC GGAGTCACCG AGGACGCGGC GAATGTCACT 
GCAACGGGAA CGGCTACGGC GGCCGCAGGA GCACCCGCTG CCACTCCAAT AGGCATAGCT 
CCGCCGCCCG TTGTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

e(y)3 ( NM_001272789.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259718.1 Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant B, mRNA.

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CloneID	OFa02152
Clone ID Related Accession (Same CDS sequence)	NM_134490.3 , NM_001272792.1 , NM_001272789.1
Accession Version	NM_001272789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of yellow 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGACTCCCT CCCAGCAAAC AGCGACGTCT GGACAACGAC 
GAGAACGACC AGCAGACGCA GAATTGCCAC GATCCCGCCA GAAAGTGCAG TGAATCACTG 
CAGGCGCTTC CGCCGTCACA TCGCTCGCGC AAGCAGAAGC ACGAGCGAAT CCTGAACACA 
GATCTGGAGG GTTCGCTTTT TCCACCCACC CAACAGCAGT CGATTTCAAC GCCGGATCAA 
AATGGAGCCT TGTCCTCGGA GGTCGAAGGC GAGGACGCAA AGGCGCAAGA TCCGCTAGAA 
CCGGCGACGC CTCAGCCCCC ACCGGTAGCG ACGCCGGCAG GAACGGGCAA GAAGCGTGGG 
CGGCCGAAGC GTATCCAAAA CCAGAGCTCG CCGGATGGTG GAGGTGCAGT TACTCCCAAA 
CCCGGCACTC CACAGGAGGA GGCCAACAGC GCTGTCAAGT CGCGTCGCGT ACAACTGCTG 
CGCAAGCGAC TGGCCATCGA CATGGTGAGC GTGGGCCAAG AGCAGGCGGA CATGAAAGCT 
AAGGAAAAGG AGTCGTCTGT GGGCGTGCCA AATGCAAGAG TTGAAGACGG GCTTGCCGAA 
ACTCTGGAAT CTCCAAAGAC TCGCGACCAT CGCCCCCTGA GAGCCACGCG CCGCACCACG 
ACCAGCACAA ACACCAACCT GCAGCCCACG CCCAAGTCCA CAAGGAAGCG CCAGAGCAAG 
GCCAGTGTCC AACAAAGCCA GTTGCCGCCG CCGACGATGG CTCAGTTCGG TAGTTCGGAG 
TCCGAGTCCA ACAACAACAA TAATAGCAGC ATATCCTTTG CCCTGAGCGC GCAGATCGAC 
CTCACAATGT GCTCGTCCAG TTCGTCGACC TCCTCTGGCG CTGCCGCCAA CCAGCAGGTG 
ATCGGTGGAA GTGGCAGCAG CTCGATGCTA CCTCCGACGA CGATTCTGTC TTCTTCGGAT 
CCGCTGCCCG ATGTCATCTT CCAGCCAAAT GACTTCTCCT CTATCATGGC CACCCAGCAG 
CTGCGCTCCT CGCGCCCCTC AAGCATCAGC TGTGGCAGTA CCGGCGGTAG CCAGCCGGAC 
TGTCACGACG AGGACAACTA CACCAGCGCG CTGGACAATT CCGGAGACGA AACTGCGTTG 
CCTATGCCCC CCACGCGGGG ACGTGGTCGT GGCCGGCGAT CCAGGGGAGG CCGCGGTAGA 
GGATCTTCTT CGGTGGATCG GGCTGTCAGC GTCGGAGGCA CAGCCTCCAC AACGCCCGCC 
ACGCAGCCTA GGGCCCCGCG AATGAGCCGA GGAGCAAGTG CCGTAGCAAA GGCCATCGCC 
ATGAGTCGAC CACGATGCGT GGGCGGCCTC AAGCACCAGC CCGATCCCGA GAGACTTAAA 
GGCTTGTTCA GTCCGTCGCC GCAAGTGTTT GAGGAGGACA CGCGTATGAG CGCCGATCTT 
AGCAACAGCA ATCAGTCGAT GGCCAGCCTG ATGGAACCCC CGCTAACGCC ACAGAAGCAA 
CCCGACTTCC TCAACAACGA GGAGTCACAG TCGTCCATGG TGAGCAATGT GAGCATGATG 
GACTCCAACC AGGGTCAGTC CGCCGACGCG ATTCTGGGTT CTGCTCTCAA ACGTCCAAAG 
AAGAAGAAGA TGGAAACTTG CGTGGCCGAA GACACCGACT ACAGCGCCTC CAGCATCGCC 
GAGTACGACT GGCCACCGCC TAAAGGTTGC TGCCCATCAA AGAATCGGGA CACCTTCATG 
ATTCAAGAGC AGGTGGCGCT CTATCTGGGC ATTACCAGCT TTAAGCGCAA GTACCCGGAT 
CTGCCACGCC GATCGGTCGA CATGGAGGAG CGCAATTGGC TGCAGGAGAA GGGGCTGGTT 
AGCGAGAAGT TGTGCGACCT GGGCATCACC GCTGTGTGGG CCTCCGACAT ATTGGACATC 
ATGTACGCGG ACTTTTTCGA CAAGTACGAA GAGTACAAGG AGTACATTCG CAGAAAGCAT 
TTGCGCGAGA TCGAGGCTAA GCAGAAGGCC CTAGGACTCA CAGTCGGGGC CGGACGAGGT 
CTGCAAGCTC GCGACCGTGC CATGCTTTCG GCCACCAAGT GGAACGCTTA CTTCAACAAG 
TCCCGTAAGG ATGAGCGCCA GTCCTGCATG GACCTGCAGA CCTTCACGAT AAATCAGCCG 
CAGCCAAGGA CCGCGCCCAC CTGTACACGC CTAGAGCGCT CCACTTCGGA TTTGATACGG 
GCAGTGGCAG AAGAGGCGAA TATCCCGGCA CCGCCTAATC TCCTGCCACC CCGCGACTAT 
GACGAGGCCT TCCGCAACTC AGACTACGCT TATCCACTCA CGGTGGTACC GGACCAGTTC 
TCAATGGCCT ACCGCCAGTT TGAGCCCGCC GAGCTCAGAG CCTACCCACT GGACACGGCG 
CTGGACAAGC CGCCGACCGA TCTGATGGCT CAGCTGCTAC AGGCAAAATC CGAAGCTGTA 
GGCAGCGATG AAATCAAAAC GTCCGCCCCG GCCCCCAAGG ATCTGGGGCA AGAGCAGTCC 
GCCATCAAAT CCGTAACGGT AACGGCGCCC GTCCGACGCA GCAGAAGGTC CACACGCCAG 
CAGACGGACA AGGTTCGCAC GGCTAGCAGC TCCAGCACGT CCTCCGCTCA ATCGGTCTCA 
TCCGCGTCCA GCGGCAACGG GAGCAGCAGT GATACGGAAA GCGGCGATGA AAGCGACTTT 
AGCAGCACAT CGAGCTGCAG CAGTTCGACG GGTGCCTCAA GTGGAGCAGG CAGCGAGGAC 
GAGGATGGGA ACGAGTGCTC ATCGTCGGTC AGGCTGTCCA CCTGCGGCGT CTGTCTGCGC 
AGTCAGCATC GCAATGCTAG GGACATGCCG GAGGCTTTCA TCCGCTGTTA CACATGCCGA 
AAGCGCGTCC ATCCCAGCTG CGTTGATATG CCACCGCGCA TGGTGGGTCG CGTGAGGAAC 
TACAATTGGC AGTGCGCCGG GTGCAAATGC TGCATCAAGT GCCGTAGCAG CCAACGTCCA 
GGAAAGATGC TCTATTGCGA GCAGTGCGAC CGAGGCTACC ACATCTACTG CTTGGGCCTC 
AGGACAGTCC CAGATGGGCG CTGGAGCTGT GAGCGCTGCT GTTTCTGCAT GCGATGCGGG 
GCCACCAAGC CGGAGGGTCT GCCCCAGGTG GCCGCTCTGT CCCAGGCGTC CGGAGGACCG 
TCGGCCAACG GGGATCGCTC AAAGGCAGCG CGCAATAAGC GCTTGAAATG GGTGCACGAG 
TACCGCATCG ATCATGTGAC GAAGATCCGG GAGCACGCCG CCATGTTCTG CGTACCATGT 
GCGCGAAACA AGCCCGCCAA GCGGCAGTCG GCGGCGGGTG CGGCCGGTGC GGCAGCAGTT 
ACTCCTGTCT TGGAGGCCAC TTCCGCGCAA ACGGATGACA GTCCGATGCC CAGTCCCGGC 
CTGACAACCA ACGGAGGACG CGCCCTTTCG CCAACGGCGG CACTGAGTCC AAAGGCAGCT 
GTGCCCGTGG CATCGCTGCC GCCCGTTCTT GAAGCGACGA CGGTGACCAC AAATATTGCG 
GGAACGATAG GCAGACGGCA GGCGGGTAAT GCTGTCAATA TTACAACGAT GCAGTGCAGC 
AGCAGCAGCA GCAGCAACTT CAGCGGGAAC GGAGTCACCG AGGACGCGGC GAATGTCACT 
GCAACGGGAA CGGCTACGGC GGCCGCAGGA GCACCCGCTG CCACTCCAAT AGGCATAGCT 
CCGCCGCCCG TTGTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259718.1
CDS	213..6233
Translation	MNDLRQQQMVAATSSSGSESGTAVESAAATSTAGSAGAAGRPQSNCSANSNAKSVAASSTSEEEQRVSSTSSPAQRDQQLNADRDREQEPEPQQQQQREEALQHQHNQPGHITSTTASPPPTLPPPTTPCDDAPSTTGASASASSASGEAPSAASAAGAAGGPMAATALEVESEERDGHKIILKLSKHANPNSNANESQPGGDERRVEPLRIQLPCGGAEGGLVAKQQDAFDADASSCSSSCAEDEVATTLGQQLRNTPHIVPKLTIRAANERRVGSVVPKLTIKLPENPAASGSNSNSGSCSAAVSGAQSAMPAKNDAHLSSLSPASASSSSASSSSSSSSSSSLAEMQTVPKLMIKTTLAGSSCISSSEELPQQQQQIPKLTIKTGGGGQEHVHTVIMTHDLNNAQSIPKLTIKTKSIEMIEDEQAAKLEQQLQPLPKLTIKNLCSPKHKVRAVLEEKPPTAASKLAIPKPTPNPTPAPMTNGGESNSSSQEFCGFSDPDADIAAPASDDVRRNSDDMVIDDSLSKEHDPKIFHNLPPMPASNGIASGGSKASKASKSAQSQHNVVDMVDLTSSPSPGSSPTHVPNNFTGRISPKGLLIDCLRMQTAIGCYVPEEADSRVRPAASPNSNILLSQLTAPAKSFTTTTPKSKSSKYPQLTERLMANGSGTGGGSVIAAESEAVVGPLPAANPAQSIIESIEILDTPDGSPRVAYMDEDSNPMLNKHLQTIHKLAMDHGVEQRMDTQDNNNENHLKRTNSEGNESPSSRLPPSKQRRLDNDENDQQTQNCHDPARKCSESLQALPPSHRSRKQKHERILNTDLEGSLFPPTQQQSISTPDQNGALSSEVEGEDAKAQDPLEPATPQPPPVATPAGTGKKRGRPKRIQNQSSPDGGGAVTPKPGTPQEEANSAVKSRRVQLLRKRLAIDMVSVGQEQADMKAKEKESSVGVPNARVEDGLAETLESPKTRDHRPLRATRRTTTSTNTNLQPTPKSTRKRQSKASVQQSQLPPPTMAQFGSSESESNNNNNSSISFALSAQIDLTMCSSSSSTSSGAAANQQVIGGSGSSSMLPPTTILSSSDPLPDVIFQPNDFSSIMATQQLRSSRPSSISCGSTGGSQPDCHDEDNYTSALDNSGDETALPMPPTRGRGRGRRSRGGRGRGSSSVDRAVSVGGTASTTPATQPRAPRMSRGASAVAKAIAMSRPRCVGGLKHQPDPERLKGLFSPSPQVFEEDTRMSADLSNSNQSMASLMEPPLTPQKQPDFLNNEESQSSMVSNVSMMDSNQGQSADAILGSALKRPKKKKMETCVAEDTDYSASSIAEYDWPPPKGCCPSKNRDTFMIQEQVALYLGITSFKRKYPDLPRRSVDMEERNWLQEKGLVSEKLCDLGITAVWASDILDIMYADFFDKYEEYKEYIRRKHLREIEAKQKALGLTVGAGRGLQARDRAMLSATKWNAYFNKSRKDERQSCMDLQTFTINQPQPRTAPTCTRLERSTSDLIRAVAEEANIPAPPNLLPPRDYDEAFRNSDYAYPLTVVPDQFSMAYRQFEPAELRAYPLDTALDKPPTDLMAQLLQAKSEAVGSDEIKTSAPAPKDLGQEQSAIKSVTVTAPVRRSRRSTRQQTDKVRTASSSSTSSAQSVSSASSGNGSSSDTESGDESDFSSTSSCSSSTGASSGAGSEDEDGNECSSSVRLSTCGVCLRSQHRNARDMPEAFIRCYTCRKRVHPSCVDMPPRMVGRVRNYNWQCAGCKCCIKCRSSQRPGKMLYCEQCDRGYHIYCLGLRTVPDGRWSCERCCFCMRCGATKPEGLPQVAALSQASGGPSANGDRSKAARNKRLKWVHEYRIDHVTKIREHAAMFCVPCARNKPAKRQSAAGAAGAAAVTPVLEATSAQTDDSPMPSPGLTTNGGRALSPTAALSPKAAVPVASLPPVLEATTVTTNIAGTIGRRQAGNAVNITTMQCSSSSSSNFSGNGVTEDAANVTATGTATAAAGAPAATPIGIAPPPVVA

Target ORF information:

RefSeq Version	NM_001272789.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272789.1

ORF Insert Sequence:

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGACTCCCT CCCAGCAAAC AGCGACGTCT GGACAACGAC 
GAGAACGACC AGCAGACGCA GAATTGCCAC GATCCCGCCA GAAAGTGCAG TGAATCACTG 
CAGGCGCTTC CGCCGTCACA TCGCTCGCGC AAGCAGAAGC ACGAGCGAAT CCTGAACACA 
GATCTGGAGG GTTCGCTTTT TCCACCCACC CAACAGCAGT CGATTTCAAC GCCGGATCAA 
AATGGAGCCT TGTCCTCGGA GGTCGAAGGC GAGGACGCAA AGGCGCAAGA TCCGCTAGAA 
CCGGCGACGC CTCAGCCCCC ACCGGTAGCG ACGCCGGCAG GAACGGGCAA GAAGCGTGGG 
CGGCCGAAGC GTATCCAAAA CCAGAGCTCG CCGGATGGTG GAGGTGCAGT TACTCCCAAA 
CCCGGCACTC CACAGGAGGA GGCCAACAGC GCTGTCAAGT CGCGTCGCGT ACAACTGCTG 
CGCAAGCGAC TGGCCATCGA CATGGTGAGC GTGGGCCAAG AGCAGGCGGA CATGAAAGCT 
AAGGAAAAGG AGTCGTCTGT GGGCGTGCCA AATGCAAGAG TTGAAGACGG GCTTGCCGAA 
ACTCTGGAAT CTCCAAAGAC TCGCGACCAT CGCCCCCTGA GAGCCACGCG CCGCACCACG 
ACCAGCACAA ACACCAACCT GCAGCCCACG CCCAAGTCCA CAAGGAAGCG CCAGAGCAAG 
GCCAGTGTCC AACAAAGCCA GTTGCCGCCG CCGACGATGG CTCAGTTCGG TAGTTCGGAG 
TCCGAGTCCA ACAACAACAA TAATAGCAGC ATATCCTTTG CCCTGAGCGC GCAGATCGAC 
CTCACAATGT GCTCGTCCAG TTCGTCGACC TCCTCTGGCG CTGCCGCCAA CCAGCAGGTG 
ATCGGTGGAA GTGGCAGCAG CTCGATGCTA CCTCCGACGA CGATTCTGTC TTCTTCGGAT 
CCGCTGCCCG ATGTCATCTT CCAGCCAAAT GACTTCTCCT CTATCATGGC CACCCAGCAG 
CTGCGCTCCT CGCGCCCCTC AAGCATCAGC TGTGGCAGTA CCGGCGGTAG CCAGCCGGAC 
TGTCACGACG AGGACAACTA CACCAGCGCG CTGGACAATT CCGGAGACGA AACTGCGTTG 
CCTATGCCCC CCACGCGGGG ACGTGGTCGT GGCCGGCGAT CCAGGGGAGG CCGCGGTAGA 
GGATCTTCTT CGGTGGATCG GGCTGTCAGC GTCGGAGGCA CAGCCTCCAC AACGCCCGCC 
ACGCAGCCTA GGGCCCCGCG AATGAGCCGA GGAGCAAGTG CCGTAGCAAA GGCCATCGCC 
ATGAGTCGAC CACGATGCGT GGGCGGCCTC AAGCACCAGC CCGATCCCGA GAGACTTAAA 
GGCTTGTTCA GTCCGTCGCC GCAAGTGTTT GAGGAGGACA CGCGTATGAG CGCCGATCTT 
AGCAACAGCA ATCAGTCGAT GGCCAGCCTG ATGGAACCCC CGCTAACGCC ACAGAAGCAA 
CCCGACTTCC TCAACAACGA GGAGTCACAG TCGTCCATGG TGAGCAATGT GAGCATGATG 
GACTCCAACC AGGGTCAGTC CGCCGACGCG ATTCTGGGTT CTGCTCTCAA ACGTCCAAAG 
AAGAAGAAGA TGGAAACTTG CGTGGCCGAA GACACCGACT ACAGCGCCTC CAGCATCGCC 
GAGTACGACT GGCCACCGCC TAAAGGTTGC TGCCCATCAA AGAATCGGGA CACCTTCATG 
ATTCAAGAGC AGGTGGCGCT CTATCTGGGC ATTACCAGCT TTAAGCGCAA GTACCCGGAT 
CTGCCACGCC GATCGGTCGA CATGGAGGAG CGCAATTGGC TGCAGGAGAA GGGGCTGGTT 
AGCGAGAAGT TGTGCGACCT GGGCATCACC GCTGTGTGGG CCTCCGACAT ATTGGACATC 
ATGTACGCGG ACTTTTTCGA CAAGTACGAA GAGTACAAGG AGTACATTCG CAGAAAGCAT 
TTGCGCGAGA TCGAGGCTAA GCAGAAGGCC CTAGGACTCA CAGTCGGGGC CGGACGAGGT 
CTGCAAGCTC GCGACCGTGC CATGCTTTCG GCCACCAAGT GGAACGCTTA CTTCAACAAG 
TCCCGTAAGG ATGAGCGCCA GTCCTGCATG GACCTGCAGA CCTTCACGAT AAATCAGCCG 
CAGCCAAGGA CCGCGCCCAC CTGTACACGC CTAGAGCGCT CCACTTCGGA TTTGATACGG 
GCAGTGGCAG AAGAGGCGAA TATCCCGGCA CCGCCTAATC TCCTGCCACC CCGCGACTAT 
GACGAGGCCT TCCGCAACTC AGACTACGCT TATCCACTCA CGGTGGTACC GGACCAGTTC 
TCAATGGCCT ACCGCCAGTT TGAGCCCGCC GAGCTCAGAG CCTACCCACT GGACACGGCG 
CTGGACAAGC CGCCGACCGA TCTGATGGCT CAGCTGCTAC AGGCAAAATC CGAAGCTGTA 
GGCAGCGATG AAATCAAAAC GTCCGCCCCG GCCCCCAAGG ATCTGGGGCA AGAGCAGTCC 
GCCATCAAAT CCGTAACGGT AACGGCGCCC GTCCGACGCA GCAGAAGGTC CACACGCCAG 
CAGACGGACA AGGTTCGCAC GGCTAGCAGC TCCAGCACGT CCTCCGCTCA ATCGGTCTCA 
TCCGCGTCCA GCGGCAACGG GAGCAGCAGT GATACGGAAA GCGGCGATGA AAGCGACTTT 
AGCAGCACAT CGAGCTGCAG CAGTTCGACG GGTGCCTCAA GTGGAGCAGG CAGCGAGGAC 
GAGGATGGGA ACGAGTGCTC ATCGTCGGTC AGGCTGTCCA CCTGCGGCGT CTGTCTGCGC 
AGTCAGCATC GCAATGCTAG GGACATGCCG GAGGCTTTCA TCCGCTGTTA CACATGCCGA 
AAGCGCGTCC ATCCCAGCTG CGTTGATATG CCACCGCGCA TGGTGGGTCG CGTGAGGAAC 
TACAATTGGC AGTGCGCCGG GTGCAAATGC TGCATCAAGT GCCGTAGCAG CCAACGTCCA 
GGAAAGATGC TCTATTGCGA GCAGTGCGAC CGAGGCTACC ACATCTACTG CTTGGGCCTC 
AGGACAGTCC CAGATGGGCG CTGGAGCTGT GAGCGCTGCT GTTTCTGCAT GCGATGCGGG 
GCCACCAAGC CGGAGGGTCT GCCCCAGGTG GCCGCTCTGT CCCAGGCGTC CGGAGGACCG 
TCGGCCAACG GGGATCGCTC AAAGGCAGCG CGCAATAAGC GCTTGAAATG GGTGCACGAG 
TACCGCATCG ATCATGTGAC GAAGATCCGG GAGCACGCCG CCATGTTCTG CGTACCATGT 
GCGCGAAACA AGCCCGCCAA GCGGCAGTCG GCGGCGGGTG CGGCCGGTGC GGCAGCAGTT 
ACTCCTGTCT TGGAGGCCAC TTCCGCGCAA ACGGATGACA GTCCGATGCC CAGTCCCGGC 
CTGACAACCA ACGGAGGACG CGCCCTTTCG CCAACGGCGG CACTGAGTCC AAAGGCAGCT 
GTGCCCGTGG CATCGCTGCC GCCCGTTCTT GAAGCGACGA CGGTGACCAC AAATATTGCG 
GGAACGATAG GCAGACGGCA GGCGGGTAAT GCTGTCAATA TTACAACGAT GCAGTGCAGC 
AGCAGCAGCA GCAGCAACTT CAGCGGGAAC GGAGTCACCG AGGACGCGGC GAATGTCACT 
GCAACGGGAA CGGCTACGGC GGCCGCAGGA GCACCCGCTG CCACTCCAAT AGGCATAGCT 
CCGCCGCCCG TTGTTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

e(y)3 ( NM_001272790.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259719.1 Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant C, mRNA.

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CloneID	OFa03167
Clone ID Related Accession (Same CDS sequence)	NM_001272790.1
Accession Version	NM_001272790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of yellow 3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGACTCCCT CCCAGCAAAC AGCGACGTCT GGACAACGAC 
GAGAACGACC AGCAGACGCA GAATTGCCAC GATCCCGCCA GAAAGTGCAG TGAATCACTG 
CAGGCGCTTC CGCCGTCACA TCGCTCGCGC AAGCAGAAGC ACGAGCGAAT CCTGAACACA 
GATCTGGAGG GTTCGCTTTT TCCACCCACC CAACAGCAGT CGATTTCAAC GCCGGATCAA 
AATGGAGCCT TGTCCTCGGA GGTCGAAGGC GAGGACGCAA AGGCGCAAGA TCCGCTAGAA 
CCGGCGACGC CTCAGCCCCC ACCGGTAGCG ACGCCGGCAG GAACGGGCAA GAAGCGTGGG 
CGGCCGAAGC GTATCCAAAA CCAGAGCTCG CCGGATGGTG GAGGTGCAGT TACTCCCAAA 
CCCGGCACTC CACAGGAGGA GGCCAACAGC GCTGTCAAGT CGCGTCGCGT ACAACTGCTG 
CGCAAGCGAC TGGCCATCGA CATGGTGAGC GTGGGCCAAG AGCAGGCGGA CATGAAAGCT 
AAGGAAAAGG AGTCGTCTGT GGGCGTGCCA AATGCAAGAG TTGAAGACGG GCTTGCCGAA 
ACTCTGGAAT CTCCAAAGAC TCGCGACCAT CGCCCCCTGA GAGCCACGCG CCGCACCACG 
ACCAGCACAA ACACCAACCT GCAGCCCACG CCCAAGTCCA CAAGGAAGCG CCAGAGCAAG 
GCCAGTGTCC AACAAAGCCA GTTGCCGCCG CCGACGATGG CTCAGTTCGG TAGTTCGGAG 
TCCGAGTCCA ACAACAACAA TAATAGCAGC ATATCCTTTG CCCTGAGCGC GCAGATCGAC 
CTCACAATGT GCTCGTCCAG TTCGTCGACC TCCTCTGGCG CTGCCGCCAA CCAGCAGGTG 
ATCGGTGGAA GTGGCAGCAG CTCGATGCTA CCTCCGACGA CGATTCTGTC TTCTTCGGAT 
CCGCTGCCCG ATGTCATCTT CCAGCCAAAT GACTTCTCCT CTATCATGGC CACCCAGCAG 
CTGCGCTCCT CGCGCCCCTC AAGCATCAGC TGTGGCAGTA CCGGCGGTAG CCAGCCGGAC 
TGTCACGACG AGGACAACTA CACCAGCGCG CTGGACAATT CCGGAGTAAA CTCTTCCTCT 
TCAGACGAAA CTGCGTTGCC TATGCCCCCC ACGCGGGGAC GTGGTCGTGG CCGGCGATCC 
AGGGGAGGCC GCGGTAGAGG ATCTTCTTCG GTGGATCGGG CTGTCAGCGT CGGAGGCACA 
GCCTCCACAA CGCCCGCCAC GCAGCCTAGG GCCCCGCGAA TGAGCCGAGG AGCAAGTGCC 
GTAGCAAAGG CCATCGCCAT GAGTCGACCA CGATGCGTGG GCGGCCTCAA GCACCAGCCC 
GATCCCGAGA GACTTAAAGG CTTGTTCAGT CCGTCGCCGC AAGTGTTTGA GGAGGACACG 
CGTATGAGCG CCGATCTTAG CAACAGCAAT CAGTCGATGG CCAGCCTGAT GGAACCCCCG 
CTAACGCCAC AGAAGCAACC CGACTTCCTC AACAACGAGG AGTCACAGTC GTCCATGGTG 
AGCAATGTGA GCATGATGGA CTCCAACCAG GGTCAGTCCG CCGACGCGAT TCTGGGTTCT 
GCTCTCAAAC GTCCAAAGAA GAAGAAGATG GAAACTTGCG TGGCCGAAGA CACCGACTAC 
AGCGCCTCCA GCATCGCCGA GTACGACTGG CCACCGCCTA AAGGTTGCTG CCCATCAAAG 
AATCGGGACA CCTTCATGAT TCAAGAGCAG GTGGCGCTCT ATCTGGGCAT TACCAGCTTT 
AAGCGCAAGT ACCCGGATCT GCCACGCCGA TCGGTCGACA TGGAGGAGCG CAATTGGCTG 
CAGGAGAAGG GGCTGGTTAG CGAGAAGTTG TGCGACCTGG GCATCACCGC TGTGTGGGCC 
TCCGACATAT TGGACATCAT GTACGCGGAC TTTTTCGACA AGTACGAAGA GTACAAGGAG 
TACATTCGCA GAAAGCATTT GCGCGAGATC GAGGCTAAGC AGAAGGCCCT AGGACTCACA 
GTCGGGGCCG GACGAGGTCT GCAAGCTCGC GACCGTGCCA TGCTTTCGGC CACCAAGTGG 
AACGCTTACT TCAACAAGTC CCGTAAGGAT GAGCGCCAGT CCTGCATGGA CCTGCAGACC 
TTCACGATAA ATCAGCCGCA GCCAAGGACC GCGCCCACCT GTACACGCCT AGAGCGCTCC 
ACTTCGGATT TGATACGGGC AGTGGCAGAA GAGGCGAATA TCCCGGCACC GCCTAATCTC 
CTGCCACCCC GCGACTATGA CGAGGCCTTC CGCAACTCAG ACTACGCTTA TCCACTCACG 
GTGGTACCGG ACCAGTTCTC AATGGCCTAC CGCCAGTTTG AGCCCGCCGA GCTCAGAGCC 
TACCCACTGG ACACGGCGCT GGACAAGCCG CCGACCGATC TGATGGCTCA GCTGCTACAG 
GCAAAATCCG AAGCTGTAGG CAGCGATGAA ATCAAAACGT CCGCCCCGGC CCCCAAGGAT 
CTGGGGCAAG AGCAGTCCGC CATCAAATCC GTAACGGTAA CGGCGCCCGT CCGACGCAGC 
AGAAGGTCCA CACGCCAGCA GACGGACAAG GTTCGCACGG CTAGCAGCTC CAGCACGTCC 
TCCGCTCAAT CGGTCTCATC CGCGTCCAGC GGCAACGGGA GCAGCAGTGA TACGGAAAGC 
GGCGATGAAA GCGACTTTAG CAGCACATCG AGCTGCAGCA GTTCGACGGG TGCCTCAAGT 
GGAGCAGGCA GCGAGGACGA GGATGGGAAC GAGTGCTCAT CGTCGGTCAG GCTGTCCACC 
TGCGGCGTCT GTCTGCGCAG TCAGCATCGC AATGCTAGGG ACATGCCGGA GGCTTTCATC 
CGCTGTTACA CATGCCGAAA GCGCGTCCAT CCCAGCTGCG TTGATATGCC ACCGCGCATG 
GTGGGTCGCG TGAGGAACTA CAATTGGCAG TGCGCCGGGT GCAAATGCTG CATCAAGTGC 
CGTAGCAGCC AACGTCCAGG AAAGATGCTC TATTGCGAGC AGTGCGACCG AGGCTACCAC 
ATCTACTGCT TGGGCCTCAG GACAGTCCCA GATGGGCGCT GGAGCTGTGA GCGCTGCTGT 
TTCTGCATGC GATGCGGGGC CACCAAGCCG GAGGGTCTGC CCCAGGTGGC CGCTCTGTCC 
CAGGCGTCCG GAGGACCGTC GGCCAACGGG GATCGCTCAA AGGCAGCGCG CAATAAGCGC 
TTGAAATGGG TGCACGAGTA CCGCATCGAT CATGTGACGA AGATCCGGGA GCACGCCGCC 
ATGTTCTGCG TACCATGTGC GCGAAACAAG CCCGCCAAGC GGCAGTCGGC GGCGGGTGCG 
GCCGGTGCGG CAGCAGTTAC TCCTGTCTTG GAGGCCACTT CCGCGCAAAC GGATGACAGT 
CCGATGCCCA GTCCCGGCCT GACAACCAAC GGAGGACGCG CCCTTTCGCC AACGGCGGCA 
CTGAGTCCAA AGGCAGCTGT GCCCGTGGCA TCGCTGCCGC CCGTTCTTGA AGCGACGACG 
GTGACCACAA ATATTGCGGG AACGATAGGC AGACGGCAGG CGGGTAATGC TGTCAATATT 
ACAACGATGC AGTGCAGCAG CAGCAGCAGC AGCAACTTCA GCGGGAACGG AGTCACCGAG 
GACGCGGCGA ATGTCACTGC AACGGGAACG GCTACGGCGG CCGCAGGAGC ACCCGCTGCC 
ACTCCAATAG GCATAGCTCC GCCGCCCGTT GTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259719.1
CDS	1030..7068
Translation	MNDLRQQQMVAATSSSGSESGTAVESAAATSTAGSAGAAGRPQSNCSANSNAKSVAASSTSEEEQRVSSTSSPAQRDQQLNADRDREQEPEPQQQQQREEALQHQHNQPGHITSTTASPPPTLPPPTTPCDDAPSTTGASASASSASGEAPSAASAAGAAGGPMAATALEVESEERDGHKIILKLSKHANPNSNANESQPGGDERRVEPLRIQLPCGGAEGGLVAKQQDAFDADASSCSSSCAEDEVATTLGQQLRNTPHIVPKLTIRAANERRVGSVVPKLTIKLPENPAASGSNSNSGSCSAAVSGAQSAMPAKNDAHLSSLSPASASSSSASSSSSSSSSSSLAEMQTVPKLMIKTTLAGSSCISSSEELPQQQQQIPKLTIKTGGGGQEHVHTVIMTHDLNNAQSIPKLTIKTKSIEMIEDEQAAKLEQQLQPLPKLTIKNLCSPKHKVRAVLEEKPPTAASKLAIPKPTPNPTPAPMTNGGESNSSSQEFCGFSDPDADIAAPASDDVRRNSDDMVIDDSLSKEHDPKIFHNLPPMPASNGIASGGSKASKASKSAQSQHNVVDMVDLTSSPSPGSSPTHVPNNFTGRISPKGLLIDCLRMQTAIGCYVPEEADSRVRPAASPNSNILLSQLTAPAKSFTTTTPKSKSSKYPQLTERLMANGSGTGGGSVIAAESEAVVGPLPAANPAQSIIESIEILDTPDGSPRVAYMDEDSNPMLNKHLQTIHKLAMDHGVEQRMDTQDNNNENHLKRTNSEGNESPSSRLPPSKQRRLDNDENDQQTQNCHDPARKCSESLQALPPSHRSRKQKHERILNTDLEGSLFPPTQQQSISTPDQNGALSSEVEGEDAKAQDPLEPATPQPPPVATPAGTGKKRGRPKRIQNQSSPDGGGAVTPKPGTPQEEANSAVKSRRVQLLRKRLAIDMVSVGQEQADMKAKEKESSVGVPNARVEDGLAETLESPKTRDHRPLRATRRTTTSTNTNLQPTPKSTRKRQSKASVQQSQLPPPTMAQFGSSESESNNNNNSSISFALSAQIDLTMCSSSSSTSSGAAANQQVIGGSGSSSMLPPTTILSSSDPLPDVIFQPNDFSSIMATQQLRSSRPSSISCGSTGGSQPDCHDEDNYTSALDNSGVNSSSSDETALPMPPTRGRGRGRRSRGGRGRGSSSVDRAVSVGGTASTTPATQPRAPRMSRGASAVAKAIAMSRPRCVGGLKHQPDPERLKGLFSPSPQVFEEDTRMSADLSNSNQSMASLMEPPLTPQKQPDFLNNEESQSSMVSNVSMMDSNQGQSADAILGSALKRPKKKKMETCVAEDTDYSASSIAEYDWPPPKGCCPSKNRDTFMIQEQVALYLGITSFKRKYPDLPRRSVDMEERNWLQEKGLVSEKLCDLGITAVWASDILDIMYADFFDKYEEYKEYIRRKHLREIEAKQKALGLTVGAGRGLQARDRAMLSATKWNAYFNKSRKDERQSCMDLQTFTINQPQPRTAPTCTRLERSTSDLIRAVAEEANIPAPPNLLPPRDYDEAFRNSDYAYPLTVVPDQFSMAYRQFEPAELRAYPLDTALDKPPTDLMAQLLQAKSEAVGSDEIKTSAPAPKDLGQEQSAIKSVTVTAPVRRSRRSTRQQTDKVRTASSSSTSSAQSVSSASSGNGSSSDTESGDESDFSSTSSCSSSTGASSGAGSEDEDGNECSSSVRLSTCGVCLRSQHRNARDMPEAFIRCYTCRKRVHPSCVDMPPRMVGRVRNYNWQCAGCKCCIKCRSSQRPGKMLYCEQCDRGYHIYCLGLRTVPDGRWSCERCCFCMRCGATKPEGLPQVAALSQASGGPSANGDRSKAARNKRLKWVHEYRIDHVTKIREHAAMFCVPCARNKPAKRQSAAGAAGAAAVTPVLEATSAQTDDSPMPSPGLTTNGGRALSPTAALSPKAAVPVASLPPVLEATTVTTNIAGTIGRRQAGNAVNITTMQCSSSSSSNFSGNGVTEDAANVTATGTATAAAGAPAATPIGIAPPPVVA

Target ORF information:

RefSeq Version	NM_001272790.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272790.1

ORF Insert Sequence:

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGACTCCCT CCCAGCAAAC AGCGACGTCT GGACAACGAC 
GAGAACGACC AGCAGACGCA GAATTGCCAC GATCCCGCCA GAAAGTGCAG TGAATCACTG 
CAGGCGCTTC CGCCGTCACA TCGCTCGCGC AAGCAGAAGC ACGAGCGAAT CCTGAACACA 
GATCTGGAGG GTTCGCTTTT TCCACCCACC CAACAGCAGT CGATTTCAAC GCCGGATCAA 
AATGGAGCCT TGTCCTCGGA GGTCGAAGGC GAGGACGCAA AGGCGCAAGA TCCGCTAGAA 
CCGGCGACGC CTCAGCCCCC ACCGGTAGCG ACGCCGGCAG GAACGGGCAA GAAGCGTGGG 
CGGCCGAAGC GTATCCAAAA CCAGAGCTCG CCGGATGGTG GAGGTGCAGT TACTCCCAAA 
CCCGGCACTC CACAGGAGGA GGCCAACAGC GCTGTCAAGT CGCGTCGCGT ACAACTGCTG 
CGCAAGCGAC TGGCCATCGA CATGGTGAGC GTGGGCCAAG AGCAGGCGGA CATGAAAGCT 
AAGGAAAAGG AGTCGTCTGT GGGCGTGCCA AATGCAAGAG TTGAAGACGG GCTTGCCGAA 
ACTCTGGAAT CTCCAAAGAC TCGCGACCAT CGCCCCCTGA GAGCCACGCG CCGCACCACG 
ACCAGCACAA ACACCAACCT GCAGCCCACG CCCAAGTCCA CAAGGAAGCG CCAGAGCAAG 
GCCAGTGTCC AACAAAGCCA GTTGCCGCCG CCGACGATGG CTCAGTTCGG TAGTTCGGAG 
TCCGAGTCCA ACAACAACAA TAATAGCAGC ATATCCTTTG CCCTGAGCGC GCAGATCGAC 
CTCACAATGT GCTCGTCCAG TTCGTCGACC TCCTCTGGCG CTGCCGCCAA CCAGCAGGTG 
ATCGGTGGAA GTGGCAGCAG CTCGATGCTA CCTCCGACGA CGATTCTGTC TTCTTCGGAT 
CCGCTGCCCG ATGTCATCTT CCAGCCAAAT GACTTCTCCT CTATCATGGC CACCCAGCAG 
CTGCGCTCCT CGCGCCCCTC AAGCATCAGC TGTGGCAGTA CCGGCGGTAG CCAGCCGGAC 
TGTCACGACG AGGACAACTA CACCAGCGCG CTGGACAATT CCGGAGTAAA CTCTTCCTCT 
TCAGACGAAA CTGCGTTGCC TATGCCCCCC ACGCGGGGAC GTGGTCGTGG CCGGCGATCC 
AGGGGAGGCC GCGGTAGAGG ATCTTCTTCG GTGGATCGGG CTGTCAGCGT CGGAGGCACA 
GCCTCCACAA CGCCCGCCAC GCAGCCTAGG GCCCCGCGAA TGAGCCGAGG AGCAAGTGCC 
GTAGCAAAGG CCATCGCCAT GAGTCGACCA CGATGCGTGG GCGGCCTCAA GCACCAGCCC 
GATCCCGAGA GACTTAAAGG CTTGTTCAGT CCGTCGCCGC AAGTGTTTGA GGAGGACACG 
CGTATGAGCG CCGATCTTAG CAACAGCAAT CAGTCGATGG CCAGCCTGAT GGAACCCCCG 
CTAACGCCAC AGAAGCAACC CGACTTCCTC AACAACGAGG AGTCACAGTC GTCCATGGTG 
AGCAATGTGA GCATGATGGA CTCCAACCAG GGTCAGTCCG CCGACGCGAT TCTGGGTTCT 
GCTCTCAAAC GTCCAAAGAA GAAGAAGATG GAAACTTGCG TGGCCGAAGA CACCGACTAC 
AGCGCCTCCA GCATCGCCGA GTACGACTGG CCACCGCCTA AAGGTTGCTG CCCATCAAAG 
AATCGGGACA CCTTCATGAT TCAAGAGCAG GTGGCGCTCT ATCTGGGCAT TACCAGCTTT 
AAGCGCAAGT ACCCGGATCT GCCACGCCGA TCGGTCGACA TGGAGGAGCG CAATTGGCTG 
CAGGAGAAGG GGCTGGTTAG CGAGAAGTTG TGCGACCTGG GCATCACCGC TGTGTGGGCC 
TCCGACATAT TGGACATCAT GTACGCGGAC TTTTTCGACA AGTACGAAGA GTACAAGGAG 
TACATTCGCA GAAAGCATTT GCGCGAGATC GAGGCTAAGC AGAAGGCCCT AGGACTCACA 
GTCGGGGCCG GACGAGGTCT GCAAGCTCGC GACCGTGCCA TGCTTTCGGC CACCAAGTGG 
AACGCTTACT TCAACAAGTC CCGTAAGGAT GAGCGCCAGT CCTGCATGGA CCTGCAGACC 
TTCACGATAA ATCAGCCGCA GCCAAGGACC GCGCCCACCT GTACACGCCT AGAGCGCTCC 
ACTTCGGATT TGATACGGGC AGTGGCAGAA GAGGCGAATA TCCCGGCACC GCCTAATCTC 
CTGCCACCCC GCGACTATGA CGAGGCCTTC CGCAACTCAG ACTACGCTTA TCCACTCACG 
GTGGTACCGG ACCAGTTCTC AATGGCCTAC CGCCAGTTTG AGCCCGCCGA GCTCAGAGCC 
TACCCACTGG ACACGGCGCT GGACAAGCCG CCGACCGATC TGATGGCTCA GCTGCTACAG 
GCAAAATCCG AAGCTGTAGG CAGCGATGAA ATCAAAACGT CCGCCCCGGC CCCCAAGGAT 
CTGGGGCAAG AGCAGTCCGC CATCAAATCC GTAACGGTAA CGGCGCCCGT CCGACGCAGC 
AGAAGGTCCA CACGCCAGCA GACGGACAAG GTTCGCACGG CTAGCAGCTC CAGCACGTCC 
TCCGCTCAAT CGGTCTCATC CGCGTCCAGC GGCAACGGGA GCAGCAGTGA TACGGAAAGC 
GGCGATGAAA GCGACTTTAG CAGCACATCG AGCTGCAGCA GTTCGACGGG TGCCTCAAGT 
GGAGCAGGCA GCGAGGACGA GGATGGGAAC GAGTGCTCAT CGTCGGTCAG GCTGTCCACC 
TGCGGCGTCT GTCTGCGCAG TCAGCATCGC AATGCTAGGG ACATGCCGGA GGCTTTCATC 
CGCTGTTACA CATGCCGAAA GCGCGTCCAT CCCAGCTGCG TTGATATGCC ACCGCGCATG 
GTGGGTCGCG TGAGGAACTA CAATTGGCAG TGCGCCGGGT GCAAATGCTG CATCAAGTGC 
CGTAGCAGCC AACGTCCAGG AAAGATGCTC TATTGCGAGC AGTGCGACCG AGGCTACCAC 
ATCTACTGCT TGGGCCTCAG GACAGTCCCA GATGGGCGCT GGAGCTGTGA GCGCTGCTGT 
TTCTGCATGC GATGCGGGGC CACCAAGCCG GAGGGTCTGC CCCAGGTGGC CGCTCTGTCC 
CAGGCGTCCG GAGGACCGTC GGCCAACGGG GATCGCTCAA AGGCAGCGCG CAATAAGCGC 
TTGAAATGGG TGCACGAGTA CCGCATCGAT CATGTGACGA AGATCCGGGA GCACGCCGCC 
ATGTTCTGCG TACCATGTGC GCGAAACAAG CCCGCCAAGC GGCAGTCGGC GGCGGGTGCG 
GCCGGTGCGG CAGCAGTTAC TCCTGTCTTG GAGGCCACTT CCGCGCAAAC GGATGACAGT 
CCGATGCCCA GTCCCGGCCT GACAACCAAC GGAGGACGCG CCCTTTCGCC AACGGCGGCA 
CTGAGTCCAA AGGCAGCTGT GCCCGTGGCA TCGCTGCCGC CCGTTCTTGA AGCGACGACG 
GTGACCACAA ATATTGCGGG AACGATAGGC AGACGGCAGG CGGGTAATGC TGTCAATATT 
ACAACGATGC AGTGCAGCAG CAGCAGCAGC AGCAACTTCA GCGGGAACGG AGTCACCGAG 
GACGCGGCGA ATGTCACTGC AACGGGAACG GCTACGGCGG CCGCAGGAGC ACCCGCTGCC 
ACTCCAATAG GCATAGCTCC GCCGCCCGTT GTTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

e(y)3 ( NM_001272791.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259720.1 Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant D, mRNA.

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CloneID	OFa03168
Clone ID Related Accession (Same CDS sequence)	NM_001272791.1
Accession Version	NM_001272791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of yellow 3, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGATTTTTC TTTTCGCAGC TCCCTCCCAG CAAACAGCGA 
CGTCTGGACA ACGACGAGAA CGACCAGCAG ACGCAGAATT GCCACGATCC CGCCAGAAAG 
TGCAGTGAAT CACTGCAGGC GCTTCCGCCG TCACATCGCT CGCGCAAGCA GAAGCACGAG 
CGAATCCTGA ACACAGATCT GGAGGGTTCG CTTTTTCCAC CCACCCAACA GCAGTCGATT 
TCAACGCCGG ATCAAAATGG AGCCTTGTCC TCGGAGGTCG AAGGCGAGGA CGCAAAGGCG 
CAAGATCCGC TAGAACCGGC GACGCCTCAG CCCCCACCGG TAGCGACGCC GGCAGGAACG 
GGCAAGAAGC GTGGGCGGCC GAAGCGTATC CAAAACCAGA GCTCGCCGGA TGGTGGAGGT 
GCAGTTACTC CCAAACCCGG CACTCCACAG GAGGAGGCCA ACAGCGCTGT CAAGTCGCGT 
CGCGTACAAC TGCTGCGCAA GCGACTGGCC ATCGACATGG TGAGCGTGGG CCAAGAGCAG 
GCGGACATGA AAGCTAAGGA AAAGGAGTCG TCTGTGGGCG TGCCAAATGC AAGAGTTGAA 
GACGGGCTTG CCGAAACTCT GGAATCTCCA AAGACTCGCG ACCATCGCCC CCTGAGAGCC 
ACGCGCCGCA CCACGACCAG CACAAACACC AACCTGCAGC CCACGCCCAA GTCCACAAGG 
AAGCGCCAGA GCAAGGCCAG TGTCCAACAA AGCCAGTTGC CGCCGCCGAC GATGGCTCAG 
TTCGGTAGTT CGGAGTCCGA GTCCAACAAC AACAATAATA GCAGCATATC CTTTGCCCTG 
AGCGCGCAGA TCGACCTCAC AATGTGCTCG TCCAGTTCGT CGACCTCCTC TGGCGCTGCC 
GCCAACCAGC AGGTGATCGG TGGAAGTGGC AGCAGCTCGA TGCTACCTCC GACGACGATT 
CTGTCTTCTT CGGATCCGCT GCCCGATGTC ATCTTCCAGC CAAATGACTT CTCCTCTATC 
ATGGCCACCC AGCAGCTGCG CTCCTCGCGC CCCTCAAGCA TCAGCTGTGG CAGTACCGGC 
GGTAGCCAGC CGGACTGTCA CGACGAGGAC AACTACACCA GCGCGCTGGA CAATTCCGGA 
GACGAAACTG CGTTGCCTAT GCCCCCCACG CGGGGACGTG GTCGTGGCCG GCGATCCAGG 
GGAGGCCGCG GTAGAGGATC TTCTTCGGTG GATCGGGCTG TCAGCGTCGG AGGCACAGCC 
TCCACAACGC CCGCCACGCA GCCTAGGGCC CCGCGAATGA GCCGAGGAGC AAGTGCCGTA 
GCAAAGGCCA TCGCCATGAG TCGACCACGA TGCGTGGGCG GCCTCAAGCA CCAGCCCGAT 
CCCGAGAGAC TTAAAGGCTT GTTCAGTCCG TCGCCGCAAG TGTTTGAGGA GGACACGCGT 
ATGAGCGCCG ATCTTAGCAA CAGCAATCAG TCGATGGCCA GCCTGATGGA ACCCCCGCTA 
ACGCCACAGA AGCAACCCGA CTTCCTCAAC AACGAGGAGT CACAGTCGTC CATGGTGAGC 
AATGTGAGCA TGATGGACTC CAACCAGGGT CAGTCCGCCG ACGCGATTCT GGGTTCTGCT 
CTCAAACGTC CAAAGAAGAA GAAGATGGAA ACTTGCGTGG CCGAAGACAC CGACTACAGC 
GCCTCCAGCA TCGCCGAGTA CGACTGGCCA CCGCCTAAAG GTTGCTGCCC ATCAAAGAAT 
CGGGACACCT TCATGATTCA AGAGCAGGTG GCGCTCTATC TGGGCATTAC CAGCTTTAAG 
CGCAAGTACC CGGATCTGCC ACGCCGATCG GTCGACATGG AGGAGCGCAA TTGGCTGCAG 
GAGAAGGGGC TGGTTAGCGA GAAGTTGTGC GACCTGGGCA TCACCGCTGT GTGGGCCTCC 
GACATATTGG ACATCATGTA CGCGGACTTT TTCGACAAGT ACGAAGAGTA CAAGGAGTAC 
ATTCGCAGAA AGCATTTGCG CGAGATCGAG GCTAAGCAGA AGGCCCTAGG ACTCACAGTC 
GGGGCCGGAC GAGGTCTGCA AGCTCGCGAC CGTGCCATGC TTTCGGCCAC CAAGTGGAAC 
GCTTACTTCA ACAAGTCCCG TAAGGATGAG CGCCAGTCCT GCATGGACCT GCAGACCTTC 
ACGATAAATC AGCCGCAGCC AAGGACCGCG CCCACCTGTA CACGCCTAGA GCGCTCCACT 
TCGGATTTGA TACGGGCAGT GGCAGAAGAG GCGAATATCC CGGCACCGCC TAATCTCCTG 
CCACCCCGCG ACTATGACGA GGCCTTCCGC AACTCAGACT ACGCTTATCC ACTCACGGTG 
GTACCGGACC AGTTCTCAAT GGCCTACCGC CAGTTTGAGC CCGCCGAGCT CAGAGCCTAC 
CCACTGGACA CGGCGCTGGA CAAGCCGCCG ACCGATCTGA TGGCTCAGCT GCTACAGGCA 
AAATCCGAAG CTGTAGGCAG CGATGAAATC AAAACGTCCG CCCCGGCCCC CAAGGATCTG 
GGGCAAGAGC AGTCCGCCAT CAAATCCGTA ACGGTAACGG CGCCCGTCCG ACGCAGCAGA 
AGGTCCACAC GCCAGCAGAC GGACAAGGTT CGCACGGCTA GCAGCTCCAG CACGTCCTCC 
GCTCAATCGG TCTCATCCGC GTCCAGCGGC AACGGGAGCA GCAGTGATAC GGAAAGCGGC 
GATGAAAGCG ACTTTAGCAG CACATCGAGC TGCAGCAGTT CGACGGGTGC CTCAAGTGGA 
GCAGGCAGCG AGGACGAGGA TGGGAACGAG TGCTCATCGT CGGTCAGGCT GTCCACCTGC 
GGCGTCTGTC TGCGCAGTCA GCATCGCAAT GCTAGGGACA TGCCGGAGGC TTTCATCCGC 
TGTTACACAT GCCGAAAGCG CGTCCATCCC AGCTGCGTTG ATATGCCACC GCGCATGGTG 
GGTCGCGTGA GGAACTACAA TTGGCAGTGC GCCGGGTGCA AATGCTGCAT CAAGTGCCGT 
AGCAGCCAAC GTCCAGGAAA GATGCTCTAT TGCGAGCAGT GCGACCGAGG CTACCACATC 
TACTGCTTGG GCCTCAGGAC AGTCCCAGAT GGGCGCTGGA GCTGTGAGCG CTGCTGTTTC 
TGCATGCGAT GCGGGGCCAC CAAGCCGGAG GGTCTGCCCC AGGTGGCCGC TCTGTCCCAG 
GCGTCCGGAG GACCGTCGGC CAACGGGGAT CGCTCAAAGG CAGCGCGCAA TAAGCGCTTG 
AAATGGGTGC ACGAGTACCG CATCGATCAT GTGACGAAGA TCCGGGAGCA CGCCGCCATG 
TTCTGCGTAC CATGTGCGCG AAACAAGCCC GCCAAGCGGC AGTCGGCGGC GGGTGCGGCC 
GGTGCGGCAG CAGTTACTCC TGTCTTGGAG GCCACTTCCG CGCAAACGGA TGACAGTCCG 
ATGCCCAGTC CCGGCCTGAC AACCAACGGA GGACGCGCCC TTTCGCCAAC GGCGGCACTG 
AGTCCAAAGG CAGCTGTGCC CGTGGCATCG CTGCCGCCCG TTCTTGAAGC GACGACGGTG 
ACCACAAATA TTGCGGGAAC GATAGGCAGA CGGCAGGCGG GTAATGCTGT CAATATTACA 
ACGATGCAGT GCAGCAGCAG CAGCAGCAGC AACTTCAGCG GGAACGGAGT CACCGAGGAC 
GCGGCGAATG TCACTGCAAC GGGAACGGCT ACGGCGGCCG CAGGAGCACC CGCTGCCACT 
CCAATAGGCA TAGCTCCGCC GCCCGTTGTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259720.1
CDS	1551..7586
Translation	MNDLRQQQMVAATSSSGSESGTAVESAAATSTAGSAGAAGRPQSNCSANSNAKSVAASSTSEEEQRVSSTSSPAQRDQQLNADRDREQEPEPQQQQQREEALQHQHNQPGHITSTTASPPPTLPPPTTPCDDAPSTTGASASASSASGEAPSAASAAGAAGGPMAATALEVESEERDGHKIILKLSKHANPNSNANESQPGGDERRVEPLRIQLPCGGAEGGLVAKQQDAFDADASSCSSSCAEDEVATTLGQQLRNTPHIVPKLTIRAANERRVGSVVPKLTIKLPENPAASGSNSNSGSCSAAVSGAQSAMPAKNDAHLSSLSPASASSSSASSSSSSSSSSSLAEMQTVPKLMIKTTLAGSSCISSSEELPQQQQQIPKLTIKTGGGGQEHVHTVIMTHDLNNAQSIPKLTIKTKSIEMIEDEQAAKLEQQLQPLPKLTIKNLCSPKHKVRAVLEEKPPTAASKLAIPKPTPNPTPAPMTNGGESNSSSQEFCGFSDPDADIAAPASDDVRRNSDDMVIDDSLSKEHDPKIFHNLPPMPASNGIASGGSKASKASKSAQSQHNVVDMVDLTSSPSPGSSPTHVPNNFTGRISPKGLLIDCLRMQTAIGCYVPEEADSRVRPAASPNSNILLSQLTAPAKSFTTTTPKSKSSKYPQLTERLMANGSGTGGGSVIAAESEAVVGPLPAANPAQSIIESIEILDTPDGSPRVAYMDEDSNPMLNKHLQTIHKLAMDHGVEQRMDTQDNNNENHLKRTNSEGNESPSSRFFFSQLPPSKQRRLDNDENDQQTQNCHDPARKCSESLQALPPSHRSRKQKHERILNTDLEGSLFPPTQQQSISTPDQNGALSSEVEGEDAKAQDPLEPATPQPPPVATPAGTGKKRGRPKRIQNQSSPDGGGAVTPKPGTPQEEANSAVKSRRVQLLRKRLAIDMVSVGQEQADMKAKEKESSVGVPNARVEDGLAETLESPKTRDHRPLRATRRTTTSTNTNLQPTPKSTRKRQSKASVQQSQLPPPTMAQFGSSESESNNNNNSSISFALSAQIDLTMCSSSSSTSSGAAANQQVIGGSGSSSMLPPTTILSSSDPLPDVIFQPNDFSSIMATQQLRSSRPSSISCGSTGGSQPDCHDEDNYTSALDNSGDETALPMPPTRGRGRGRRSRGGRGRGSSSVDRAVSVGGTASTTPATQPRAPRMSRGASAVAKAIAMSRPRCVGGLKHQPDPERLKGLFSPSPQVFEEDTRMSADLSNSNQSMASLMEPPLTPQKQPDFLNNEESQSSMVSNVSMMDSNQGQSADAILGSALKRPKKKKMETCVAEDTDYSASSIAEYDWPPPKGCCPSKNRDTFMIQEQVALYLGITSFKRKYPDLPRRSVDMEERNWLQEKGLVSEKLCDLGITAVWASDILDIMYADFFDKYEEYKEYIRRKHLREIEAKQKALGLTVGAGRGLQARDRAMLSATKWNAYFNKSRKDERQSCMDLQTFTINQPQPRTAPTCTRLERSTSDLIRAVAEEANIPAPPNLLPPRDYDEAFRNSDYAYPLTVVPDQFSMAYRQFEPAELRAYPLDTALDKPPTDLMAQLLQAKSEAVGSDEIKTSAPAPKDLGQEQSAIKSVTVTAPVRRSRRSTRQQTDKVRTASSSSTSSAQSVSSASSGNGSSSDTESGDESDFSSTSSCSSSTGASSGAGSEDEDGNECSSSVRLSTCGVCLRSQHRNARDMPEAFIRCYTCRKRVHPSCVDMPPRMVGRVRNYNWQCAGCKCCIKCRSSQRPGKMLYCEQCDRGYHIYCLGLRTVPDGRWSCERCCFCMRCGATKPEGLPQVAALSQASGGPSANGDRSKAARNKRLKWVHEYRIDHVTKIREHAAMFCVPCARNKPAKRQSAAGAAGAAAVTPVLEATSAQTDDSPMPSPGLTTNGGRALSPTAALSPKAAVPVASLPPVLEATTVTTNIAGTIGRRQAGNAVNITTMQCSSSSSSNFSGNGVTEDAANVTATGTATAAAGAPAATPIGIAPPPVVA

Target ORF information:

RefSeq Version	NM_001272791.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of yellow 3 (e(y)3), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272791.1

ORF Insert Sequence:

ATGAATGATT TACGTCAGCA GCAGATGGTA GCAGCGACAT CGTCATCAGG ATCGGAATCG 
GGAACAGCGG TAGAATCGGC AGCCGCCACG TCCACAGCAG GATCAGCCGG AGCAGCAGGT 
CGCCCCCAGT CTAACTGTAG CGCTAATAGC AACGCCAAGT CAGTGGCAGC GAGCAGCACG 
AGCGAGGAGG AGCAGCGAGT TAGCTCCACG TCGTCGCCAG CCCAGCGGGA TCAGCAGCTG 
AATGCAGATC GGGACCGGGA ACAGGAGCCA GAGCCACAGC AGCAGCAGCA GCGGGAGGAG 
GCACTGCAGC ACCAGCATAA TCAGCCCGGC CATATAACCA GCACCACAGC GTCGCCTCCG 
CCGACGCTGC CACCGCCGAC GACGCCGTGC GACGATGCCC CAAGCACAAC TGGAGCATCT 
GCATCGGCCA GCTCAGCATC CGGAGAAGCA CCATCAGCAG CATCAGCAGC GGGAGCAGCA 
GGTGGACCGA TGGCCGCTAC GGCGCTTGAG GTAGAGAGCG AGGAGCGGGA TGGACATAAG 
ATCATCCTGA AGCTCTCCAA GCACGCCAAT CCCAACTCAA ACGCAAATGA ATCTCAGCCG 
GGCGGAGATG AGAGGCGCGT GGAGCCCCTG CGCATTCAGC TGCCCTGCGG CGGAGCGGAA 
GGAGGCTTGG TTGCGAAGCA GCAAGATGCC TTCGATGCGG ACGCCTCTTC CTGCTCCTCG 
TCGTGCGCCG AGGACGAGGT GGCTACCACC CTCGGCCAAC AACTACGAAA CACCCCGCAC 
ATTGTGCCAA AGCTGACCAT CCGCGCCGCG AACGAGAGGC GCGTAGGCAG CGTGGTGCCT 
AAGCTGACCA TCAAGTTGCC TGAGAATCCG GCTGCTTCCG GCTCCAACTC CAACTCTGGA 
TCGTGCTCAG CAGCTGTGAG TGGCGCCCAG TCAGCTATGC CCGCCAAGAA CGATGCCCAC 
CTGTCCAGCC TCTCGCCCGC ATCCGCCTCG TCTTCATCTG CCTCGTCCTC CTCCTCATCG 
TCGTCGTCGT CCTCACTGGC GGAGATGCAG ACGGTGCCCA AGCTGATGAT CAAGACAACG 
CTAGCCGGTA GTAGCTGCAT CAGCAGCAGT GAGGAGCTTC CGCAGCAGCA GCAGCAGATA 
CCAAAGTTAA CAATCAAGAC AGGTGGCGGC GGTCAGGAGC ACGTGCACAC GGTCATAATG 
ACGCACGACC TAAACAACGC CCAGAGCATC CCCAAACTCA CCATTAAAAC CAAATCCATA 
GAGATGATCG AGGACGAACA GGCGGCTAAG CTGGAGCAGC AATTGCAGCC GCTGCCCAAG 
CTGACCATCA AGAACTTATG CTCACCGAAG CACAAGGTGC GTGCAGTGCT CGAGGAGAAG 
CCACCAACGG CGGCCTCCAA GCTGGCTATC CCAAAACCCA CGCCCAATCC CACGCCGGCT 
CCCATGACGA ATGGCGGCGA GTCGAACAGC AGTAGTCAGG AATTCTGCGG CTTCTCCGAC 
CCAGACGCAG ATATAGCTGC GCCAGCCTCG GACGACGTTC GCCGCAATTC GGACGACATG 
GTGATCGACG ATTCGCTCTC CAAGGAGCAC GATCCCAAGA TTTTTCACAA TCTACCGCCC 
ATGCCCGCCA GCAACGGCAT AGCCAGCGGT GGGAGTAAGG CCAGTAAAGC CAGCAAGTCT 
GCCCAATCCC AGCACAACGT TGTGGACATG GTGGACCTGA CCTCGTCACC GTCGCCGGGC 
TCTTCGCCAA CGCATGTGCC TAACAACTTC ACGGGACGCA TTTCGCCGAA AGGGTTGCTC 
ATCGACTGCC TGCGGATGCA AACTGCGATC GGCTGTTATG TGCCCGAGGA GGCCGACAGT 
CGCGTTAGGC CAGCTGCGTC GCCCAATTCG AACATATTGC TTAGCCAGCT GACGGCACCG 
GCTAAAAGCT TCACAACGAC CACGCCTAAA AGCAAATCCA GCAAGTATCC CCAGCTAACG 
GAGCGCCTGA TGGCGAATGG AAGCGGTACC GGTGGGGGAT CTGTTATTGC TGCAGAAAGC 
GAGGCAGTCG TTGGTCCCTT GCCCGCCGCG AATCCCGCCC AATCCATAAT CGAGTCCATT 
GAAATCCTGG ACACGCCTGA TGGCAGCCCG AGGGTAGCCT ACATGGACGA GGATTCTAAC 
CCGATGCTCA ACAAACATCT CCAAACTATT CACAAGCTCG CAATGGATCA CGGAGTGGAA 
CAGCGTATGG ATACCCAGGA CAACAACAAC GAGAACCACT TGAAACGCAC TAACAGCGAG 
GGAAACGAAT CTCCCAGCAG TAGATTTTTC TTTTCGCAGC TCCCTCCCAG CAAACAGCGA 
CGTCTGGACA ACGACGAGAA CGACCAGCAG ACGCAGAATT GCCACGATCC CGCCAGAAAG 
TGCAGTGAAT CACTGCAGGC GCTTCCGCCG TCACATCGCT CGCGCAAGCA GAAGCACGAG 
CGAATCCTGA ACACAGATCT GGAGGGTTCG CTTTTTCCAC CCACCCAACA GCAGTCGATT 
TCAACGCCGG ATCAAAATGG AGCCTTGTCC TCGGAGGTCG AAGGCGAGGA CGCAAAGGCG 
CAAGATCCGC TAGAACCGGC GACGCCTCAG CCCCCACCGG TAGCGACGCC GGCAGGAACG 
GGCAAGAAGC GTGGGCGGCC GAAGCGTATC CAAAACCAGA GCTCGCCGGA TGGTGGAGGT 
GCAGTTACTC CCAAACCCGG CACTCCACAG GAGGAGGCCA ACAGCGCTGT CAAGTCGCGT 
CGCGTACAAC TGCTGCGCAA GCGACTGGCC ATCGACATGG TGAGCGTGGG CCAAGAGCAG 
GCGGACATGA AAGCTAAGGA AAAGGAGTCG TCTGTGGGCG TGCCAAATGC AAGAGTTGAA 
GACGGGCTTG CCGAAACTCT GGAATCTCCA AAGACTCGCG ACCATCGCCC CCTGAGAGCC 
ACGCGCCGCA CCACGACCAG CACAAACACC AACCTGCAGC CCACGCCCAA GTCCACAAGG 
AAGCGCCAGA GCAAGGCCAG TGTCCAACAA AGCCAGTTGC CGCCGCCGAC GATGGCTCAG 
TTCGGTAGTT CGGAGTCCGA GTCCAACAAC AACAATAATA GCAGCATATC CTTTGCCCTG 
AGCGCGCAGA TCGACCTCAC AATGTGCTCG TCCAGTTCGT CGACCTCCTC TGGCGCTGCC 
GCCAACCAGC AGGTGATCGG TGGAAGTGGC AGCAGCTCGA TGCTACCTCC GACGACGATT 
CTGTCTTCTT CGGATCCGCT GCCCGATGTC ATCTTCCAGC CAAATGACTT CTCCTCTATC 
ATGGCCACCC AGCAGCTGCG CTCCTCGCGC CCCTCAAGCA TCAGCTGTGG CAGTACCGGC 
GGTAGCCAGC CGGACTGTCA CGACGAGGAC AACTACACCA GCGCGCTGGA CAATTCCGGA 
GACGAAACTG CGTTGCCTAT GCCCCCCACG CGGGGACGTG GTCGTGGCCG GCGATCCAGG 
GGAGGCCGCG GTAGAGGATC TTCTTCGGTG GATCGGGCTG TCAGCGTCGG AGGCACAGCC 
TCCACAACGC CCGCCACGCA GCCTAGGGCC CCGCGAATGA GCCGAGGAGC AAGTGCCGTA 
GCAAAGGCCA TCGCCATGAG TCGACCACGA TGCGTGGGCG GCCTCAAGCA CCAGCCCGAT 
CCCGAGAGAC TTAAAGGCTT GTTCAGTCCG TCGCCGCAAG TGTTTGAGGA GGACACGCGT 
ATGAGCGCCG ATCTTAGCAA CAGCAATCAG TCGATGGCCA GCCTGATGGA ACCCCCGCTA 
ACGCCACAGA AGCAACCCGA CTTCCTCAAC AACGAGGAGT CACAGTCGTC CATGGTGAGC 
AATGTGAGCA TGATGGACTC CAACCAGGGT CAGTCCGCCG ACGCGATTCT GGGTTCTGCT 
CTCAAACGTC CAAAGAAGAA GAAGATGGAA ACTTGCGTGG CCGAAGACAC CGACTACAGC 
GCCTCCAGCA TCGCCGAGTA CGACTGGCCA CCGCCTAAAG GTTGCTGCCC ATCAAAGAAT 
CGGGACACCT TCATGATTCA AGAGCAGGTG GCGCTCTATC TGGGCATTAC CAGCTTTAAG 
CGCAAGTACC CGGATCTGCC ACGCCGATCG GTCGACATGG AGGAGCGCAA TTGGCTGCAG 
GAGAAGGGGC TGGTTAGCGA GAAGTTGTGC GACCTGGGCA TCACCGCTGT GTGGGCCTCC 
GACATATTGG ACATCATGTA CGCGGACTTT TTCGACAAGT ACGAAGAGTA CAAGGAGTAC 
ATTCGCAGAA AGCATTTGCG CGAGATCGAG GCTAAGCAGA AGGCCCTAGG ACTCACAGTC 
GGGGCCGGAC GAGGTCTGCA AGCTCGCGAC CGTGCCATGC TTTCGGCCAC CAAGTGGAAC 
GCTTACTTCA ACAAGTCCCG TAAGGATGAG CGCCAGTCCT GCATGGACCT GCAGACCTTC 
ACGATAAATC AGCCGCAGCC AAGGACCGCG CCCACCTGTA CACGCCTAGA GCGCTCCACT 
TCGGATTTGA TACGGGCAGT GGCAGAAGAG GCGAATATCC CGGCACCGCC TAATCTCCTG 
CCACCCCGCG ACTATGACGA GGCCTTCCGC AACTCAGACT ACGCTTATCC ACTCACGGTG 
GTACCGGACC AGTTCTCAAT GGCCTACCGC CAGTTTGAGC CCGCCGAGCT CAGAGCCTAC 
CCACTGGACA CGGCGCTGGA CAAGCCGCCG ACCGATCTGA TGGCTCAGCT GCTACAGGCA 
AAATCCGAAG CTGTAGGCAG CGATGAAATC AAAACGTCCG CCCCGGCCCC CAAGGATCTG 
GGGCAAGAGC AGTCCGCCAT CAAATCCGTA ACGGTAACGG CGCCCGTCCG ACGCAGCAGA 
AGGTCCACAC GCCAGCAGAC GGACAAGGTT CGCACGGCTA GCAGCTCCAG CACGTCCTCC 
GCTCAATCGG TCTCATCCGC GTCCAGCGGC AACGGGAGCA GCAGTGATAC GGAAAGCGGC 
GATGAAAGCG ACTTTAGCAG CACATCGAGC TGCAGCAGTT CGACGGGTGC CTCAAGTGGA 
GCAGGCAGCG AGGACGAGGA TGGGAACGAG TGCTCATCGT CGGTCAGGCT GTCCACCTGC 
GGCGTCTGTC TGCGCAGTCA GCATCGCAAT GCTAGGGACA TGCCGGAGGC TTTCATCCGC 
TGTTACACAT GCCGAAAGCG CGTCCATCCC AGCTGCGTTG ATATGCCACC GCGCATGGTG 
GGTCGCGTGA GGAACTACAA TTGGCAGTGC GCCGGGTGCA AATGCTGCAT CAAGTGCCGT 
AGCAGCCAAC GTCCAGGAAA GATGCTCTAT TGCGAGCAGT GCGACCGAGG CTACCACATC 
TACTGCTTGG GCCTCAGGAC AGTCCCAGAT GGGCGCTGGA GCTGTGAGCG CTGCTGTTTC 
TGCATGCGAT GCGGGGCCAC CAAGCCGGAG GGTCTGCCCC AGGTGGCCGC TCTGTCCCAG 
GCGTCCGGAG GACCGTCGGC CAACGGGGAT CGCTCAAAGG CAGCGCGCAA TAAGCGCTTG 
AAATGGGTGC ACGAGTACCG CATCGATCAT GTGACGAAGA TCCGGGAGCA CGCCGCCATG 
TTCTGCGTAC CATGTGCGCG AAACAAGCCC GCCAAGCGGC AGTCGGCGGC GGGTGCGGCC 
GGTGCGGCAG CAGTTACTCC TGTCTTGGAG GCCACTTCCG CGCAAACGGA TGACAGTCCG 
ATGCCCAGTC CCGGCCTGAC AACCAACGGA GGACGCGCCC TTTCGCCAAC GGCGGCACTG 
AGTCCAAAGG CAGCTGTGCC CGTGGCATCG CTGCCGCCCG TTCTTGAAGC GACGACGGTG 
ACCACAAATA TTGCGGGAAC GATAGGCAGA CGGCAGGCGG GTAATGCTGT CAATATTACA 
ACGATGCAGT GCAGCAGCAG CAGCAGCAGC AACTTCAGCG GGAACGGAGT CACCGAGGAC 
GCGGCGAATG TCACTGCAAC GGGAACGGCT ACGGCGGCCG CAGGAGCACC CGCTGCCACT 
CCAATAGGCA TAGCTCCGCC GCCCGTTGTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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e(y)3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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