unc-4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	unc-4
Entrez Gene ID	32757
Full Name	CG6269 gene product from transcript CG6269-RC
Synonyms	CG6269,DPHD-1,Dmel\CG6269,Dunc-4,Unc4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 16C10-16D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298460.1	NP_001285389.1	unc-4, isoform B
NM_133014.3	NP_573242.2	unc-4, isoform C
NM_001298461.1	NP_001285390.1	unc-4, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001495	XP_001238516.3
Drosophila melanogaster (fruit fly)	unc-4	NP_573242.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following unc-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa21525	NM_133014.3 Latest version!	Drosophila melanogaster unc-4, transcript variant C (unc-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21525	NM_001298460.1 Latest version!	Drosophila melanogaster unc-4, transcript variant B (unc-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21526	NM_001298461.1 Latest version!	Drosophila melanogaster unc-4, transcript variant D (unc-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc-4 ( NM_001298460.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285389.1 Drosophila melanogaster unc-4, transcript variant B (unc-4), mRNA.

Price & Availability↑

CloneID	OFa21525
Clone ID Related Accession (Same CDS sequence)	NM_001298460.1 , NM_133014.3
Accession Version	NM_001298460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTTGG AGACCTCCGA GGGCGATACC AAAAAGTTCC TGACCAACAA CAATAACTCG 
AGTGCCAGCC GAAATAACAA CAGCCACAAT AACAACAACA ACAATCACAG TCCCAAGGAA 
ATTCCGGAGG AGACTGGCCG GAGTAGCAGC ACCAGCAGTA ACAGCATCCC CAACGCCCAC 
CGGACCAACG CCGGTCAGCA TCTGTTGGGT GGCTCCCCAT CGAGCGCCTG CTCCACATCC 
GTATCCGGTT GCGGCATGCC CTCCGAGGGC CTCCATCCGA CGGCCGCACT CCAGCTTTAT 
GCCGCCGCCG CCCAACTGGC GCCTAATGGC GTCCGCGTAC CGCCCTGGGG TCCCTTCCTT 
CAGTTCGGCG TGCCGGGCGT CTTTGGTCCG AACGGACCCT TCCTCGGGCG GCCCCGTTTC 
GATGCCGCCT CCGCCGGCGG ACATCCGAAT TCCGCTGCTG CCGCCGCGGC CGCCACGCAA 
ATGGCCGCTG TGAATGCTAG CAATGCGTTC GCCAATCTAA CGGGACTGAG TGCGGCGGCG 
CTGCGAAACG TGAGTGCCGC CCAAACGACG GCGGTGGCGG CCGTCGCCAG TACAGTGGCC 
ACCATCCAGC ACCGACTGAT GATCGGCAAC CGGCAGAGTT TGCCGCCGGC GGGACCACCC 
AGCGAAGGAT CTAATGAGGA TGGCGGTTTT CCCGGCGACG GCGACGACGA CAGCAGTGCG 
GCCAAGCGGC GCAGGTCGCG CACCAACTTC AACTCCTGGC AACTGGAGGA GCTGGAGAGG 
GCCTTCTCCG CCAGCCACTA TCCGGACATC TTCATGCGGG AGGCCCTGGC CATGCGGCTG 
GACCTCAAGG AGTCGCGAGT TGCGGTATGG TTCCAGAATC GACGTGCCAA GGTGCGTAAG 
CGCGAGCACA CGAAGAAGGG ACCCGGCCGC CCCGCCCACA ACGCCCAGCC GCAGACCTGC 
AGCGGTGAGC CCATTCCGCC CAACGAGCTC AAGGCCAAAG AGCGAGCCCG TCGTCGAAAG 
AAGCTGGCCA AGGCCATCGA CCGGCAGGCC CGGAAACTGC AGGCCAAGGG CATCACCGTG 
GACCTGGAGG CCCTCAAGGC CGAGTACATA TCACAGCACA AGGCGAACGG CACCTTCTCC 
GATTCGGACC TGGAGGACGA TGGCATCCAG ATCGACGTGG TGGGCGGCAC GGACAGCGAC 
GACGAGGGCG ACAGCGACGC CGTCAGTCCG GTGCGTCTCG GTGGCGGAGG AGGTGGTGGC 
GGTGGCACTG GAGGCGGCGG AGGTGGTGGA GCCTCCTCGT CGCTCCACTG CGGACTGGAT 
GGCGATGGCG ACAGTTCGCG GGCGGGCAGC TTTATCGGCG GCGGCGGTAG CCTGGGCAGC 
CCCAGTTCGG TGGCCCCACC CTCGATGCTC TCCAATGGAG CGGTCCAGTT CGGCAAGCTG 
GAGCCAATGG ATGGCAATGA GTCCGAGGAG CGGGACAGGG AGCGCGACTC GCCAAAGCCG 
CTCCTCTTTC CCGCCAAGGC CTTCCATCAA CTGAACCTGC AGAGCAGCCA ACAGCACCAC 
GGTCTGGTGG TGGGCCAGGG TCAGGGATCG GGATCTGGAC ACGGGCACGG GCACCAGCAC 
AACCACCATC AGCACCACCT TCATCATGGC AGTGCATCCG CCTCGGCGGC GGCAGCAGTG 
GCCAATCTGG TGCACCAGAC ATCGCCGATC AGCATGCGCC GGAGCAATCC CTTCAGCATC 
GAGTCCTTGC TGTTCAACAA CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285389.1
CDS	363..2129
Translation	MVLETSEGDTKKFLTNNNNSSASRNNNSHNNNNNNHSPKEIPEETGRSSSTSSNSIPNAHRTNAGQHLLGGSPSSACSTSVSGCGMPSEGLHPTAALQLYAAAAQLAPNGVRVPPWGPFLQFGVPGVFGPNGPFLGRPRFDAASAGGHPNSAAAAAAATQMAAVNASNAFANLTGLSAAALRNVSAAQTTAVAAVASTVATIQHRLMIGNRQSLPPAGPPSEGSNEDGGFPGDGDDDSSAAKRRRSRTNFNSWQLEELERAFSASHYPDIFMREALAMRLDLKESRVAVWFQNRRAKVRKREHTKKGPGRPAHNAQPQTCSGEPIPPNELKAKERARRRKKLAKAIDRQARKLQAKGITVDLEALKAEYISQHKANGTFSDSDLEDDGIQIDVVGGTDSDDEGDSDAVSPVRLGGGGGGGGGTGGGGGGGASSSLHCGLDGDGDSSRAGSFIGGGGSLGSPSSVAPPSMLSNGAVQFGKLEPMDGNESEERDRERDSPKPLLFPAKAFHQLNLQSSQQHHGLVVGQGQGSGSGHGHGHQHNHHQHHLHHGSASASAAAAVANLVHQTSPISMRRSNPFSIESLLFNNT

Target ORF information:

RefSeq Version	NM_001298460.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-4, transcript variant B (unc-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298460.1

ORF Insert Sequence:

ATGGTTTTGG AGACCTCCGA GGGCGATACC AAAAAGTTCC TGACCAACAA CAATAACTCG 
AGTGCCAGCC GAAATAACAA CAGCCACAAT AACAACAACA ACAATCACAG TCCCAAGGAA 
ATTCCGGAGG AGACTGGCCG GAGTAGCAGC ACCAGCAGTA ACAGCATCCC CAACGCCCAC 
CGGACCAACG CCGGTCAGCA TCTGTTGGGT GGCTCCCCAT CGAGCGCCTG CTCCACATCC 
GTATCCGGTT GCGGCATGCC CTCCGAGGGC CTCCATCCGA CGGCCGCACT CCAGCTTTAT 
GCCGCCGCCG CCCAACTGGC GCCTAATGGC GTCCGCGTAC CGCCCTGGGG TCCCTTCCTT 
CAGTTCGGCG TGCCGGGCGT CTTTGGTCCG AACGGACCCT TCCTCGGGCG GCCCCGTTTC 
GATGCCGCCT CCGCCGGCGG ACATCCGAAT TCCGCTGCTG CCGCCGCGGC CGCCACGCAA 
ATGGCCGCTG TGAATGCTAG CAATGCGTTC GCCAATCTAA CGGGACTGAG TGCGGCGGCG 
CTGCGAAACG TGAGTGCCGC CCAAACGACG GCGGTGGCGG CCGTCGCCAG TACAGTGGCC 
ACCATCCAGC ACCGACTGAT GATCGGCAAC CGGCAGAGTT TGCCGCCGGC GGGACCACCC 
AGCGAAGGAT CTAATGAGGA TGGCGGTTTT CCCGGCGACG GCGACGACGA CAGCAGTGCG 
GCCAAGCGGC GCAGGTCGCG CACCAACTTC AACTCCTGGC AACTGGAGGA GCTGGAGAGG 
GCCTTCTCCG CCAGCCACTA TCCGGACATC TTCATGCGGG AGGCCCTGGC CATGCGGCTG 
GACCTCAAGG AGTCGCGAGT TGCGGTATGG TTCCAGAATC GACGTGCCAA GGTGCGTAAG 
CGCGAGCACA CGAAGAAGGG ACCCGGCCGC CCCGCCCACA ACGCCCAGCC GCAGACCTGC 
AGCGGTGAGC CCATTCCGCC CAACGAGCTC AAGGCCAAAG AGCGAGCCCG TCGTCGAAAG 
AAGCTGGCCA AGGCCATCGA CCGGCAGGCC CGGAAACTGC AGGCCAAGGG CATCACCGTG 
GACCTGGAGG CCCTCAAGGC CGAGTACATA TCACAGCACA AGGCGAACGG CACCTTCTCC 
GATTCGGACC TGGAGGACGA TGGCATCCAG ATCGACGTGG TGGGCGGCAC GGACAGCGAC 
GACGAGGGCG ACAGCGACGC CGTCAGTCCG GTGCGTCTCG GTGGCGGAGG AGGTGGTGGC 
GGTGGCACTG GAGGCGGCGG AGGTGGTGGA GCCTCCTCGT CGCTCCACTG CGGACTGGAT 
GGCGATGGCG ACAGTTCGCG GGCGGGCAGC TTTATCGGCG GCGGCGGTAG CCTGGGCAGC 
CCCAGTTCGG TGGCCCCACC CTCGATGCTC TCCAATGGAG CGGTCCAGTT CGGCAAGCTG 
GAGCCAATGG ATGGCAATGA GTCCGAGGAG CGGGACAGGG AGCGCGACTC GCCAAAGCCG 
CTCCTCTTTC CCGCCAAGGC CTTCCATCAA CTGAACCTGC AGAGCAGCCA ACAGCACCAC 
GGTCTGGTGG TGGGCCAGGG TCAGGGATCG GGATCTGGAC ACGGGCACGG GCACCAGCAC 
AACCACCATC AGCACCACCT TCATCATGGC AGTGCATCCG CCTCGGCGGC GGCAGCAGTG 
GCCAATCTGG TGCACCAGAC ATCGCCGATC AGCATGCGCC GGAGCAATCC CTTCAGCATC 
GAGTCCTTGC TGTTCAACAA CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-4 ( NM_133014.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573242.2 Drosophila melanogaster unc-4, transcript variant C (unc-4), mRNA.

Price & Availability↑

CloneID	OFa21525
Clone ID Related Accession (Same CDS sequence)	NM_001298460.1 , NM_133014.3
Accession Version	NM_133014.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_133014.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTTGG AGACCTCCGA GGGCGATACC AAAAAGTTCC TGACCAACAA CAATAACTCG 
AGTGCCAGCC GAAATAACAA CAGCCACAAT AACAACAACA ACAATCACAG TCCCAAGGAA 
ATTCCGGAGG AGACTGGCCG GAGTAGCAGC ACCAGCAGTA ACAGCATCCC CAACGCCCAC 
CGGACCAACG CCGGTCAGCA TCTGTTGGGT GGCTCCCCAT CGAGCGCCTG CTCCACATCC 
GTATCCGGTT GCGGCATGCC CTCCGAGGGC CTCCATCCGA CGGCCGCACT CCAGCTTTAT 
GCCGCCGCCG CCCAACTGGC GCCTAATGGC GTCCGCGTAC CGCCCTGGGG TCCCTTCCTT 
CAGTTCGGCG TGCCGGGCGT CTTTGGTCCG AACGGACCCT TCCTCGGGCG GCCCCGTTTC 
GATGCCGCCT CCGCCGGCGG ACATCCGAAT TCCGCTGCTG CCGCCGCGGC CGCCACGCAA 
ATGGCCGCTG TGAATGCTAG CAATGCGTTC GCCAATCTAA CGGGACTGAG TGCGGCGGCG 
CTGCGAAACG TGAGTGCCGC CCAAACGACG GCGGTGGCGG CCGTCGCCAG TACAGTGGCC 
ACCATCCAGC ACCGACTGAT GATCGGCAAC CGGCAGAGTT TGCCGCCGGC GGGACCACCC 
AGCGAAGGAT CTAATGAGGA TGGCGGTTTT CCCGGCGACG GCGACGACGA CAGCAGTGCG 
GCCAAGCGGC GCAGGTCGCG CACCAACTTC AACTCCTGGC AACTGGAGGA GCTGGAGAGG 
GCCTTCTCCG CCAGCCACTA TCCGGACATC TTCATGCGGG AGGCCCTGGC CATGCGGCTG 
GACCTCAAGG AGTCGCGAGT TGCGGTATGG TTCCAGAATC GACGTGCCAA GGTGCGTAAG 
CGCGAGCACA CGAAGAAGGG ACCCGGCCGC CCCGCCCACA ACGCCCAGCC GCAGACCTGC 
AGCGGTGAGC CCATTCCGCC CAACGAGCTC AAGGCCAAAG AGCGAGCCCG TCGTCGAAAG 
AAGCTGGCCA AGGCCATCGA CCGGCAGGCC CGGAAACTGC AGGCCAAGGG CATCACCGTG 
GACCTGGAGG CCCTCAAGGC CGAGTACATA TCACAGCACA AGGCGAACGG CACCTTCTCC 
GATTCGGACC TGGAGGACGA TGGCATCCAG ATCGACGTGG TGGGCGGCAC GGACAGCGAC 
GACGAGGGCG ACAGCGACGC CGTCAGTCCG GTGCGTCTCG GTGGCGGAGG AGGTGGTGGC 
GGTGGCACTG GAGGCGGCGG AGGTGGTGGA GCCTCCTCGT CGCTCCACTG CGGACTGGAT 
GGCGATGGCG ACAGTTCGCG GGCGGGCAGC TTTATCGGCG GCGGCGGTAG CCTGGGCAGC 
CCCAGTTCGG TGGCCCCACC CTCGATGCTC TCCAATGGAG CGGTCCAGTT CGGCAAGCTG 
GAGCCAATGG ATGGCAATGA GTCCGAGGAG CGGGACAGGG AGCGCGACTC GCCAAAGCCG 
CTCCTCTTTC CCGCCAAGGC CTTCCATCAA CTGAACCTGC AGAGCAGCCA ACAGCACCAC 
GGTCTGGTGG TGGGCCAGGG TCAGGGATCG GGATCTGGAC ACGGGCACGG GCACCAGCAC 
AACCACCATC AGCACCACCT TCATCATGGC AGTGCATCCG CCTCGGCGGC GGCAGCAGTG 
GCCAATCTGG TGCACCAGAC ATCGCCGATC AGCATGCGCC GGAGCAATCC CTTCAGCATC 
GAGTCCTTGC TGTTCAACAA CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573242.2
CDS	363..2129
Translation	MVLETSEGDTKKFLTNNNNSSASRNNNSHNNNNNNHSPKEIPEETGRSSSTSSNSIPNAHRTNAGQHLLGGSPSSACSTSVSGCGMPSEGLHPTAALQLYAAAAQLAPNGVRVPPWGPFLQFGVPGVFGPNGPFLGRPRFDAASAGGHPNSAAAAAAATQMAAVNASNAFANLTGLSAAALRNVSAAQTTAVAAVASTVATIQHRLMIGNRQSLPPAGPPSEGSNEDGGFPGDGDDDSSAAKRRRSRTNFNSWQLEELERAFSASHYPDIFMREALAMRLDLKESRVAVWFQNRRAKVRKREHTKKGPGRPAHNAQPQTCSGEPIPPNELKAKERARRRKKLAKAIDRQARKLQAKGITVDLEALKAEYISQHKANGTFSDSDLEDDGIQIDVVGGTDSDDEGDSDAVSPVRLGGGGGGGGGTGGGGGGGASSSLHCGLDGDGDSSRAGSFIGGGGSLGSPSSVAPPSMLSNGAVQFGKLEPMDGNESEERDRERDSPKPLLFPAKAFHQLNLQSSQQHHGLVVGQGQGSGSGHGHGHQHNHHQHHLHHGSASASAAAAVANLVHQTSPISMRRSNPFSIESLLFNNT

Target ORF information:

RefSeq Version	NM_133014.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-4, transcript variant C (unc-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133014.3

ORF Insert Sequence:

ATGGTTTTGG AGACCTCCGA GGGCGATACC AAAAAGTTCC TGACCAACAA CAATAACTCG 
AGTGCCAGCC GAAATAACAA CAGCCACAAT AACAACAACA ACAATCACAG TCCCAAGGAA 
ATTCCGGAGG AGACTGGCCG GAGTAGCAGC ACCAGCAGTA ACAGCATCCC CAACGCCCAC 
CGGACCAACG CCGGTCAGCA TCTGTTGGGT GGCTCCCCAT CGAGCGCCTG CTCCACATCC 
GTATCCGGTT GCGGCATGCC CTCCGAGGGC CTCCATCCGA CGGCCGCACT CCAGCTTTAT 
GCCGCCGCCG CCCAACTGGC GCCTAATGGC GTCCGCGTAC CGCCCTGGGG TCCCTTCCTT 
CAGTTCGGCG TGCCGGGCGT CTTTGGTCCG AACGGACCCT TCCTCGGGCG GCCCCGTTTC 
GATGCCGCCT CCGCCGGCGG ACATCCGAAT TCCGCTGCTG CCGCCGCGGC CGCCACGCAA 
ATGGCCGCTG TGAATGCTAG CAATGCGTTC GCCAATCTAA CGGGACTGAG TGCGGCGGCG 
CTGCGAAACG TGAGTGCCGC CCAAACGACG GCGGTGGCGG CCGTCGCCAG TACAGTGGCC 
ACCATCCAGC ACCGACTGAT GATCGGCAAC CGGCAGAGTT TGCCGCCGGC GGGACCACCC 
AGCGAAGGAT CTAATGAGGA TGGCGGTTTT CCCGGCGACG GCGACGACGA CAGCAGTGCG 
GCCAAGCGGC GCAGGTCGCG CACCAACTTC AACTCCTGGC AACTGGAGGA GCTGGAGAGG 
GCCTTCTCCG CCAGCCACTA TCCGGACATC TTCATGCGGG AGGCCCTGGC CATGCGGCTG 
GACCTCAAGG AGTCGCGAGT TGCGGTATGG TTCCAGAATC GACGTGCCAA GGTGCGTAAG 
CGCGAGCACA CGAAGAAGGG ACCCGGCCGC CCCGCCCACA ACGCCCAGCC GCAGACCTGC 
AGCGGTGAGC CCATTCCGCC CAACGAGCTC AAGGCCAAAG AGCGAGCCCG TCGTCGAAAG 
AAGCTGGCCA AGGCCATCGA CCGGCAGGCC CGGAAACTGC AGGCCAAGGG CATCACCGTG 
GACCTGGAGG CCCTCAAGGC CGAGTACATA TCACAGCACA AGGCGAACGG CACCTTCTCC 
GATTCGGACC TGGAGGACGA TGGCATCCAG ATCGACGTGG TGGGCGGCAC GGACAGCGAC 
GACGAGGGCG ACAGCGACGC CGTCAGTCCG GTGCGTCTCG GTGGCGGAGG AGGTGGTGGC 
GGTGGCACTG GAGGCGGCGG AGGTGGTGGA GCCTCCTCGT CGCTCCACTG CGGACTGGAT 
GGCGATGGCG ACAGTTCGCG GGCGGGCAGC TTTATCGGCG GCGGCGGTAG CCTGGGCAGC 
CCCAGTTCGG TGGCCCCACC CTCGATGCTC TCCAATGGAG CGGTCCAGTT CGGCAAGCTG 
GAGCCAATGG ATGGCAATGA GTCCGAGGAG CGGGACAGGG AGCGCGACTC GCCAAAGCCG 
CTCCTCTTTC CCGCCAAGGC CTTCCATCAA CTGAACCTGC AGAGCAGCCA ACAGCACCAC 
GGTCTGGTGG TGGGCCAGGG TCAGGGATCG GGATCTGGAC ACGGGCACGG GCACCAGCAC 
AACCACCATC AGCACCACCT TCATCATGGC AGTGCATCCG CCTCGGCGGC GGCAGCAGTG 
GCCAATCTGG TGCACCAGAC ATCGCCGATC AGCATGCGCC GGAGCAATCC CTTCAGCATC 
GAGTCCTTGC TGTTCAACAA CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-4 ( NM_001298461.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285390.1 Drosophila melanogaster unc-4, transcript variant D (unc-4), mRNA.

Price & Availability↑

CloneID	OFa21526
Clone ID Related Accession (Same CDS sequence)	NM_001298461.1
Accession Version	NM_001298461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-4, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTTGG AGACCTCCGA GGGCGATACC AAAAAGTTCC TGACCAACAA CAATAACTCG 
AGTGCCAGCC GAAATAACAA CAGCCACAAT AACAACAACA ACAATCACAG TCCCAAGGAA 
ATTCCGGAGG AGACTGGCCG GAGTAGCAGC ACCAGCAGTA ACAGCATCCC CAACGCCCAC 
CGGACCAACG CCGGTCAGCA TCTGTTGGGT GGCTCCCCAT CGAGCGCCTG CTCCACATCC 
GTATCCGGTT GCGGCATGCC CTCCGAGGGC CTCCATCCGA CGGCCGCACT CCAGCTTTAT 
GCCGCCGCCG CCCAACTGGC GCCTAATGGC GTCCGCGTAC CGCCCTGGGG TCCCTTCCTT 
CAGTTCGGCG TGCCGGGCGT CTTTGGTCCG AACGGACCCT TCCTCGGGCG GCCCCGTTTC 
GATGCCGCCT CCGCCGGCGG ACATCCGAAT TCCGCTGCTG CCGCCGCGGC CGCCACGCAA 
ATGGCCGCTG TGAATGCTAG CAATGCGTTC GCCAATCTAA CGGGACTGAG TGCGGCGGCG 
CTGCGAAACG TGAGTGCCGC CCAAACGACG GCGGTGGCGG CCGTCGCCAG TACAGTGGCC 
ACCATCCAGC ACCGACTGAT GATCGGCAAC CGGCAGAGTT TGCCGCCGGC GGGACCACCC 
AGCGAAGGAT CTAATGAGGA TGGCGGTTTT CCCGGCGACG GCGACGACGA CAGCAGTGCG 
GCCAAGCGGC GCAGGTCGCG CACCAACTTC AACTCCTGGC AACTGGAGGA GCTGGAGAGG 
GCCTTCTCCG CCAGCCACTA TCCGGACATC TTCATGCGGG AGGCCCTGGC CATGCGGCTG 
GACCTCAAGG AGTCGCGAGT TGCGATAGGT ATGGTTCCAG AATCGACGTG CCAAGGTGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285390.1
CDS	363..1265
Translation	MVLETSEGDTKKFLTNNNNSSASRNNNSHNNNNNNHSPKEIPEETGRSSSTSSNSIPNAHRTNAGQHLLGGSPSSACSTSVSGCGMPSEGLHPTAALQLYAAAAQLAPNGVRVPPWGPFLQFGVPGVFGPNGPFLGRPRFDAASAGGHPNSAAAAAAATQMAAVNASNAFANLTGLSAAALRNVSAAQTTAVAAVASTVATIQHRLMIGNRQSLPPAGPPSEGSNEDGGFPGDGDDDSSAAKRRRSRTNFNSWQLEELERAFSASHYPDIFMREALAMRLDLKESRVAIGMVPESTCQGA

Target ORF information:

RefSeq Version	NM_001298461.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-4, transcript variant D (unc-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298461.1

ORF Insert Sequence:

ATGGTTTTGG AGACCTCCGA GGGCGATACC AAAAAGTTCC TGACCAACAA CAATAACTCG 
AGTGCCAGCC GAAATAACAA CAGCCACAAT AACAACAACA ACAATCACAG TCCCAAGGAA 
ATTCCGGAGG AGACTGGCCG GAGTAGCAGC ACCAGCAGTA ACAGCATCCC CAACGCCCAC 
CGGACCAACG CCGGTCAGCA TCTGTTGGGT GGCTCCCCAT CGAGCGCCTG CTCCACATCC 
GTATCCGGTT GCGGCATGCC CTCCGAGGGC CTCCATCCGA CGGCCGCACT CCAGCTTTAT 
GCCGCCGCCG CCCAACTGGC GCCTAATGGC GTCCGCGTAC CGCCCTGGGG TCCCTTCCTT 
CAGTTCGGCG TGCCGGGCGT CTTTGGTCCG AACGGACCCT TCCTCGGGCG GCCCCGTTTC 
GATGCCGCCT CCGCCGGCGG ACATCCGAAT TCCGCTGCTG CCGCCGCGGC CGCCACGCAA 
ATGGCCGCTG TGAATGCTAG CAATGCGTTC GCCAATCTAA CGGGACTGAG TGCGGCGGCG 
CTGCGAAACG TGAGTGCCGC CCAAACGACG GCGGTGGCGG CCGTCGCCAG TACAGTGGCC 
ACCATCCAGC ACCGACTGAT GATCGGCAAC CGGCAGAGTT TGCCGCCGGC GGGACCACCC 
AGCGAAGGAT CTAATGAGGA TGGCGGTTTT CCCGGCGACG GCGACGACGA CAGCAGTGCG 
GCCAAGCGGC GCAGGTCGCG CACCAACTTC AACTCCTGGC AACTGGAGGA GCTGGAGAGG 
GCCTTCTCCG CCAGCCACTA TCCGGACATC TTCATGCGGG AGGCCCTGGC CATGCGGCTG 
GACCTCAAGG AGTCGCGAGT TGCGATAGGT ATGGTTCCAG AATCGACGTG CCAAGGTGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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unc-4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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