Ankle2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ankle2
Entrez Gene ID	32732
Full Name	Ankyrin repeat and LEM domain containing 2
Synonyms	CG8465,Dmel\CG8465,anon-WO03040301.175,anon-WO03040301.177,anon-WO03040301.179,dAnkle2,l(1)G0222,l(1)G0316
General protein information	Preferred Names Ankyrin repeat and LEM domain containing 2 Names CG8465 gene product from transcript CG8465-RG ANKLE2 ortholog Ankle2-PD Ankle2-PE Ankle2-PF Ankle2-PG CG8465-PD CG8465-PE CG8465-PF CG8465-PG ankyrin repeat and LEM domain containing 2 l(1)G0222 lethal (1) G0222
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 16B5-16B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298446.1	NP_001285375.1	ankyrin repeat and LEM domain containing 2, isoform G
NM_167569.2	NP_728082.2	ankyrin repeat and LEM domain containing 2, isoform E
NM_132993.3	NP_573221.2	ankyrin repeat and LEM domain containing 2, isoform D
NM_167570.2	NP_728083.2	ankyrin repeat and LEM domain containing 2, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	l(1)G0222	NP_573221.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011534	XP_309974.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ankle2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankle2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01913	NM_167569.2 Latest version!	Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant E (Ankle2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa01913	NM_132993.3 Latest version!	Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant D (Ankle2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa01914	NM_167570.2 Latest version!	Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant F (Ankle2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21517	NM_001298446.1 Latest version!	Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant G (Ankle2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ankle2 ( NM_167569.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728082.2 Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant E (Ankle2), mRNA.

Price & Availability↑

CloneID	OFa01913
Clone ID Related Accession (Same CDS sequence)	NM_167569.2 , NM_132993.3
Accession Version	NM_167569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ankyrin repeat and LEM domain containing 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_167569.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACCT ATTTCGGGGT CTATATCCCG ACCTCCAAAG CGGGCTGTTT TGAGGGATCG 
GTGTCGCAGT GCATCGGCTC CATAGCCGCG GTGAACATAA AGCCATCCAA TCCGGCGTCT 
GGATCGGCAT CAGTAGCATC GGGATCGCCA TCCGGCTCGG CGGCATCCGT GCAAACGGGC 
AACGCAGACG ATGGCAGTGC TGCCACCAAG TACGAGGATC CCGACTATCC ACCGGACTCG 
CCACTGTGGC TGATCTTCAC GGAGAAATCC AAGGCGCTGG ACATCCTGCG ACACTACAAG 
GAGGCGCGCC TCCGCGAGTT TCCCAATCTG GAGCAGGCGG AGAGTTACGT TCAGTTTGGG 
TTCGAGAGCA TCGAGGCGCT CAAGAGATTT TGCAAGGCAA AGCCCGAAAG CAAGCCCATT 
CCGATAATCA GCGGTAGCGG TTACAAGAGC TCACCGACCT CGACGGACAA TTCGTGCTCC 
TCCTCGCCGA CGGGTAACGG CAGTGGCTTC ATCATTCCCC TGGGAAGCAA TTCCTCAATG 
TCGAATTTAC TGCTCAGTGA CTCACCGACT TCCTCGCCGA GCAGCTCCAG CAACGTCATT 
GCCAATGGGC GACAGCAGCA GATGCAGCAG CAACAGCAGC AGCAGCCGCA GCAGCCGGAT 
GTGTCCGGAG AAGGCCCTCC TTTCCGGGCG CCCACCAAAC AGGAACTGGT AGAGTTTCGC 
AAGCAAATCG AAGGTGGTCA CATAGACCGG GTGAAGAGGA TTATATGGGA GAATCCACGA 
TTTTTGATCA GCAGCGGTGA TACGCCCACC AGTTTGAAGG AGGGCTGTCG CTATAATGCC 
ATGCACATCT GCGCCCAGGT CAATAAGGCC AGGATCGCTC AGTTGCTGTT AAAGACCATT 
TCGGATCGGG AGTTCACTCA GCTTTACGTT GGCAAGAAGG GCAGTGGCAA GATGTGTGCT 
GCCCTCAACA TCAGTCTCCT GGACTATTAC CTGAACATGC CGGACAAGGG GCGCGGCGAA 
ACACCGCTCC ACTTTGCCGC AAAGAACGGT CATGTGGCCA TGGTCGAGGT TCTCGTTTCC 
TATCCGGAGT GCAAATCGCT GCGGAATCAT GAGGGCAAGG AGCCCAAGGA AATCATCTGC 
CTGCGTAATG CTAATGCTAC ACATGTGACC ATCAAGAAGC TGGAGCTGCT CTTGTACGAT 
CCGCATTTTG TGCCCGTACT AAGATCCCAG TCAAATACAC TGCCGCCAAA AGTGGGTCAA 
CCGTTCTCGC CCAAAGATCC ACCGAACCTG CAACACAAAG CGGACGATTA CGAGGGCCTC 
AGCGTGGACC TGGCAATCAG TGCGCTGGCG GGACCCATGT CCCGCGAAAA GGCCATGAAC 
TTCTATCGCC GTTGGAAGAC ACCACCGCGG GTCAGCAACA ATGTGATGTC GCCGCTGGCT 
GGTTCACCAT TTAGCTCGCC GGTGAAAGTA ACCCCAAGCA AGTCGATCTT TGACCGAAGT 
GCTGGAAACT CGAGTCCAGT CCACTCAGGA CGCAGAGTGC TCTTTAGTCC ATTGGCGGAG 
GCGACCAGCT CACCAAAACC GACGAAAAAC GTGCCCAATG GCACCAATGA GTGCGAGCAC 
AACAATAATA ATGTGAAGCC AGTGTATCCG TTGGAGTTCC CGGCGACACC CATTCGAAAA 
ATGAAACCGG ATTTATTCAT GGCCTATCGC AATAACAATA GCTTTGATTC GCCATCTTTG 
GCCGATGACT CCCAAATCCT GGACATGAGC CTAAGCCGCA GCCTGAATGC GTCGCTAAAT 
GACAGCTTCC GTGAGCGGCA CATCAAGAAC ACTGATATCG AGAAGGGTCT GGAGGTGGTC 
GGCCGCCAAC TGGCACGACA GGAGCAGTTA GAGTGGCGCG AGTACTGGGA TTTTCTCGAT 
TCATTTTTGG ACATTGGTAC GACCGAAGGC CTGGCCCGTC TTGAAGCGTA TTTCCTGGAA 
AAGACCGAAC AGCAGGCGGA TAAATCAGAA ACGGTCTGGA ACTTTGCCCA TCTGCATCAG 
TATTTCGATT CGATGGCCGG CGAGCAACAG CAGCAACTCC GAAAGGATAA AAATGAGGCT 
GCGGGAGCAA CTTCGCCATC CGCCGGAGTC ATGACTCCGT ACACATGCGT AGAGAAGTCG 
CTGCAAGTGT TCGCCAAGCG CATCACTAAA ACGTTGATCA ACAAAATCGG CAACATGGTG 
TCCATCAACG ACACGCTGCT CTGTGAGCTC AAAAGACTGA AATCGCTGAT TGTCAGCTTC 
AAGGATGATG CCCGCTTCAT TAGCGTGGAC TTTAGCAAGG TGCATTCACG TATCGCCCAC 
CTGGTGGCCA GCTATGTGAC CCACTCGCAG GAGGTCAGCG TAGCCATGCG TCTACAATTG 
TTGCAGATGC TCCGAAGTTT GCGGCAACTG CTGGCCGACG AGCGTGGTCG AGAACAGCAT 
TTGGGCTGCG TGTGCGCTAG TCTATTGCTG ATGCTGGAAC AGGCGCCGAC ATCCGCCGTG 
CATCTACCAG ACACTCTGAA GACCGAGGAG CTATGTTGCG CCGCCTGGGA GACGGAGCAG 
TGTTGCGCCT GTCTGTGGGA CGCAAATCTC AGCCGTAAGA CCAGTCGTCG AAAGCGCACT 
AAGTCGCTGC GGGCAGCTGC TGTTGTTCAG TCTCAGGGTC AGCTTCAGGA TACTTCGGGA 
TCGACAGGGT CGTCCGCCTT GCACGCTTCG CTTGGTGTGG GATCGACCAG TTTGGGAGCA 
TCGAGGGTCG TGGCGTCCGC TTCGAAAGAT GCTTGGCGCC GTCAACAAAG CGACGACGAG 
GACTACGACA GCGATGAGCA AGTAATCTTT TTCGACTGCA CTAATGTTAC GCTGCCTTAT 
GGAAGCAGCA GCGAGGACGA GGAAAACTTC CGTACGCCGC CGCAAAGCTT GTCGCCAGGT 
ATTTCCATGG ATTTGGAGCC GCGTTACGAG TTGTTTATTT TTGGAAACGA GCCAACCAAG 
CGAGATTTGG ATGTGCTGAA TGCCCTTTCC AATGTCGACA TTGATAAGGA AACACTGCCG 
CATGTCTACG CCTGGAAGAC TGCCATGGAG AGCTACTCCT GTGCTGAAAT GAATCTCTTC 
CCATCCCCAA GGAACGTCAA GGTTCAAAAG CCGGAGCCTT GGTATTCTGG AACCAGTTCT 
AGCCACAACA GCCAACCATT GTTGCATCCC AAGCGTCTGC TTGCCACGCC AAAGCTGAAT 
GCCGTGGTCA GCGGCAGACG CGGATCCGGA CCATTGACGG CGCCAGTTAC ACCGCGTCTG 
GCGCGAACTC CGTCCGCCGC CAGTATTCAA GTTGCATCCG AGACGAATGG CGAGTCGGTC 
GGAACTGCTG TGACTCCGGC ATCGCCGATT TTGAGTTTTG CCGCCTTGAC GGCAGCGACG 
CAGTCATTCC AAACACCATT GAACAAGGTG CGCGGCTTGT TCAGCCAATA TCGGGATCAA 
CGGTCCTATA ACGAGGGGGA CACGCCGCTG GGCAATCGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728082.2
CDS	155..3679
Translation	MSTYFGVYIPTSKAGCFEGSVSQCIGSIAAVNIKPSNPASGSASVASGSPSGSAASVQTGNADDGSAATKYEDPDYPPDSPLWLIFTEKSKALDILRHYKEARLREFPNLEQAESYVQFGFESIEALKRFCKAKPESKPIPIISGSGYKSSPTSTDNSCSSSPTGNGSGFIIPLGSNSSMSNLLLSDSPTSSPSSSSNVIANGRQQQMQQQQQQQPQQPDVSGEGPPFRAPTKQELVEFRKQIEGGHIDRVKRIIWENPRFLISSGDTPTSLKEGCRYNAMHICAQVNKARIAQLLLKTISDREFTQLYVGKKGSGKMCAALNISLLDYYLNMPDKGRGETPLHFAAKNGHVAMVEVLVSYPECKSLRNHEGKEPKEIICLRNANATHVTIKKLELLLYDPHFVPVLRSQSNTLPPKVGQPFSPKDPPNLQHKADDYEGLSVDLAISALAGPMSREKAMNFYRRWKTPPRVSNNVMSPLAGSPFSSPVKVTPSKSIFDRSAGNSSPVHSGRRVLFSPLAEATSSPKPTKNVPNGTNECEHNNNNVKPVYPLEFPATPIRKMKPDLFMAYRNNNSFDSPSLADDSQILDMSLSRSLNASLNDSFRERHIKNTDIEKGLEVVGRQLARQEQLEWREYWDFLDSFLDIGTTEGLARLEAYFLEKTEQQADKSETVWNFAHLHQYFDSMAGEQQQQLRKDKNEAAGATSPSAGVMTPYTCVEKSLQVFAKRITKTLINKIGNMVSINDTLLCELKRLKSLIVSFKDDARFISVDFSKVHSRIAHLVASYVTHSQEVSVAMRLQLLQMLRSLRQLLADERGREQHLGCVCASLLLMLEQAPTSAVHLPDTLKTEELCCAAWETEQCCACLWDANLSRKTSRRKRTKSLRAAAVVQSQGQLQDTSGSTGSSALHASLGVGSTSLGASRVVASASKDAWRRQQSDDEDYDSDEQVIFFDCTNVTLPYGSSSEDEENFRTPPQSLSPGISMDLEPRYELFIFGNEPTKRDLDVLNALSNVDIDKETLPHVYAWKTAMESYSCAEMNLFPSPRNVKVQKPEPWYSGTSSSHNSQPLLHPKRLLATPKLNAVVSGRRGSGPLTAPVTPRLARTPSAASIQVASETNGESVGTAVTPASPILSFAALTAATQSFQTPLNKVRGLFSQYRDQRSYNEGDTPLGNRN

Target ORF information:

RefSeq Version	NM_167569.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant E (Ankle2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167569.2

ORF Insert Sequence:

ATGTCGACCT ATTTCGGGGT CTATATCCCG ACCTCCAAAG CGGGCTGTTT TGAGGGATCG 
GTGTCGCAGT GCATCGGCTC CATAGCCGCG GTGAACATAA AGCCATCCAA TCCGGCGTCT 
GGATCGGCAT CAGTAGCATC GGGATCGCCA TCCGGCTCGG CGGCATCCGT GCAAACGGGC 
AACGCAGACG ATGGCAGTGC TGCCACCAAG TACGAGGATC CCGACTATCC ACCGGACTCG 
CCACTGTGGC TGATCTTCAC GGAGAAATCC AAGGCGCTGG ACATCCTGCG ACACTACAAG 
GAGGCGCGCC TCCGCGAGTT TCCCAATCTG GAGCAGGCGG AGAGTTACGT TCAGTTTGGG 
TTCGAGAGCA TCGAGGCGCT CAAGAGATTT TGCAAGGCAA AGCCCGAAAG CAAGCCCATT 
CCGATAATCA GCGGTAGCGG TTACAAGAGC TCACCGACCT CGACGGACAA TTCGTGCTCC 
TCCTCGCCGA CGGGTAACGG CAGTGGCTTC ATCATTCCCC TGGGAAGCAA TTCCTCAATG 
TCGAATTTAC TGCTCAGTGA CTCACCGACT TCCTCGCCGA GCAGCTCCAG CAACGTCATT 
GCCAATGGGC GACAGCAGCA GATGCAGCAG CAACAGCAGC AGCAGCCGCA GCAGCCGGAT 
GTGTCCGGAG AAGGCCCTCC TTTCCGGGCG CCCACCAAAC AGGAACTGGT AGAGTTTCGC 
AAGCAAATCG AAGGTGGTCA CATAGACCGG GTGAAGAGGA TTATATGGGA GAATCCACGA 
TTTTTGATCA GCAGCGGTGA TACGCCCACC AGTTTGAAGG AGGGCTGTCG CTATAATGCC 
ATGCACATCT GCGCCCAGGT CAATAAGGCC AGGATCGCTC AGTTGCTGTT AAAGACCATT 
TCGGATCGGG AGTTCACTCA GCTTTACGTT GGCAAGAAGG GCAGTGGCAA GATGTGTGCT 
GCCCTCAACA TCAGTCTCCT GGACTATTAC CTGAACATGC CGGACAAGGG GCGCGGCGAA 
ACACCGCTCC ACTTTGCCGC AAAGAACGGT CATGTGGCCA TGGTCGAGGT TCTCGTTTCC 
TATCCGGAGT GCAAATCGCT GCGGAATCAT GAGGGCAAGG AGCCCAAGGA AATCATCTGC 
CTGCGTAATG CTAATGCTAC ACATGTGACC ATCAAGAAGC TGGAGCTGCT CTTGTACGAT 
CCGCATTTTG TGCCCGTACT AAGATCCCAG TCAAATACAC TGCCGCCAAA AGTGGGTCAA 
CCGTTCTCGC CCAAAGATCC ACCGAACCTG CAACACAAAG CGGACGATTA CGAGGGCCTC 
AGCGTGGACC TGGCAATCAG TGCGCTGGCG GGACCCATGT CCCGCGAAAA GGCCATGAAC 
TTCTATCGCC GTTGGAAGAC ACCACCGCGG GTCAGCAACA ATGTGATGTC GCCGCTGGCT 
GGTTCACCAT TTAGCTCGCC GGTGAAAGTA ACCCCAAGCA AGTCGATCTT TGACCGAAGT 
GCTGGAAACT CGAGTCCAGT CCACTCAGGA CGCAGAGTGC TCTTTAGTCC ATTGGCGGAG 
GCGACCAGCT CACCAAAACC GACGAAAAAC GTGCCCAATG GCACCAATGA GTGCGAGCAC 
AACAATAATA ATGTGAAGCC AGTGTATCCG TTGGAGTTCC CGGCGACACC CATTCGAAAA 
ATGAAACCGG ATTTATTCAT GGCCTATCGC AATAACAATA GCTTTGATTC GCCATCTTTG 
GCCGATGACT CCCAAATCCT GGACATGAGC CTAAGCCGCA GCCTGAATGC GTCGCTAAAT 
GACAGCTTCC GTGAGCGGCA CATCAAGAAC ACTGATATCG AGAAGGGTCT GGAGGTGGTC 
GGCCGCCAAC TGGCACGACA GGAGCAGTTA GAGTGGCGCG AGTACTGGGA TTTTCTCGAT 
TCATTTTTGG ACATTGGTAC GACCGAAGGC CTGGCCCGTC TTGAAGCGTA TTTCCTGGAA 
AAGACCGAAC AGCAGGCGGA TAAATCAGAA ACGGTCTGGA ACTTTGCCCA TCTGCATCAG 
TATTTCGATT CGATGGCCGG CGAGCAACAG CAGCAACTCC GAAAGGATAA AAATGAGGCT 
GCGGGAGCAA CTTCGCCATC CGCCGGAGTC ATGACTCCGT ACACATGCGT AGAGAAGTCG 
CTGCAAGTGT TCGCCAAGCG CATCACTAAA ACGTTGATCA ACAAAATCGG CAACATGGTG 
TCCATCAACG ACACGCTGCT CTGTGAGCTC AAAAGACTGA AATCGCTGAT TGTCAGCTTC 
AAGGATGATG CCCGCTTCAT TAGCGTGGAC TTTAGCAAGG TGCATTCACG TATCGCCCAC 
CTGGTGGCCA GCTATGTGAC CCACTCGCAG GAGGTCAGCG TAGCCATGCG TCTACAATTG 
TTGCAGATGC TCCGAAGTTT GCGGCAACTG CTGGCCGACG AGCGTGGTCG AGAACAGCAT 
TTGGGCTGCG TGTGCGCTAG TCTATTGCTG ATGCTGGAAC AGGCGCCGAC ATCCGCCGTG 
CATCTACCAG ACACTCTGAA GACCGAGGAG CTATGTTGCG CCGCCTGGGA GACGGAGCAG 
TGTTGCGCCT GTCTGTGGGA CGCAAATCTC AGCCGTAAGA CCAGTCGTCG AAAGCGCACT 
AAGTCGCTGC GGGCAGCTGC TGTTGTTCAG TCTCAGGGTC AGCTTCAGGA TACTTCGGGA 
TCGACAGGGT CGTCCGCCTT GCACGCTTCG CTTGGTGTGG GATCGACCAG TTTGGGAGCA 
TCGAGGGTCG TGGCGTCCGC TTCGAAAGAT GCTTGGCGCC GTCAACAAAG CGACGACGAG 
GACTACGACA GCGATGAGCA AGTAATCTTT TTCGACTGCA CTAATGTTAC GCTGCCTTAT 
GGAAGCAGCA GCGAGGACGA GGAAAACTTC CGTACGCCGC CGCAAAGCTT GTCGCCAGGT 
ATTTCCATGG ATTTGGAGCC GCGTTACGAG TTGTTTATTT TTGGAAACGA GCCAACCAAG 
CGAGATTTGG ATGTGCTGAA TGCCCTTTCC AATGTCGACA TTGATAAGGA AACACTGCCG 
CATGTCTACG CCTGGAAGAC TGCCATGGAG AGCTACTCCT GTGCTGAAAT GAATCTCTTC 
CCATCCCCAA GGAACGTCAA GGTTCAAAAG CCGGAGCCTT GGTATTCTGG AACCAGTTCT 
AGCCACAACA GCCAACCATT GTTGCATCCC AAGCGTCTGC TTGCCACGCC AAAGCTGAAT 
GCCGTGGTCA GCGGCAGACG CGGATCCGGA CCATTGACGG CGCCAGTTAC ACCGCGTCTG 
GCGCGAACTC CGTCCGCCGC CAGTATTCAA GTTGCATCCG AGACGAATGG CGAGTCGGTC 
GGAACTGCTG TGACTCCGGC ATCGCCGATT TTGAGTTTTG CCGCCTTGAC GGCAGCGACG 
CAGTCATTCC AAACACCATT GAACAAGGTG CGCGGCTTGT TCAGCCAATA TCGGGATCAA 
CGGTCCTATA ACGAGGGGGA CACGCCGCTG GGCAATCGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankle2 ( NM_132993.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573221.2 Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant D (Ankle2), mRNA.

Price & Availability↑

CloneID	OFa01913
Clone ID Related Accession (Same CDS sequence)	NM_167569.2 , NM_132993.3
Accession Version	NM_132993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ankyrin repeat and LEM domain containing 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_132993.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACCT ATTTCGGGGT CTATATCCCG ACCTCCAAAG CGGGCTGTTT TGAGGGATCG 
GTGTCGCAGT GCATCGGCTC CATAGCCGCG GTGAACATAA AGCCATCCAA TCCGGCGTCT 
GGATCGGCAT CAGTAGCATC GGGATCGCCA TCCGGCTCGG CGGCATCCGT GCAAACGGGC 
AACGCAGACG ATGGCAGTGC TGCCACCAAG TACGAGGATC CCGACTATCC ACCGGACTCG 
CCACTGTGGC TGATCTTCAC GGAGAAATCC AAGGCGCTGG ACATCCTGCG ACACTACAAG 
GAGGCGCGCC TCCGCGAGTT TCCCAATCTG GAGCAGGCGG AGAGTTACGT TCAGTTTGGG 
TTCGAGAGCA TCGAGGCGCT CAAGAGATTT TGCAAGGCAA AGCCCGAAAG CAAGCCCATT 
CCGATAATCA GCGGTAGCGG TTACAAGAGC TCACCGACCT CGACGGACAA TTCGTGCTCC 
TCCTCGCCGA CGGGTAACGG CAGTGGCTTC ATCATTCCCC TGGGAAGCAA TTCCTCAATG 
TCGAATTTAC TGCTCAGTGA CTCACCGACT TCCTCGCCGA GCAGCTCCAG CAACGTCATT 
GCCAATGGGC GACAGCAGCA GATGCAGCAG CAACAGCAGC AGCAGCCGCA GCAGCCGGAT 
GTGTCCGGAG AAGGCCCTCC TTTCCGGGCG CCCACCAAAC AGGAACTGGT AGAGTTTCGC 
AAGCAAATCG AAGGTGGTCA CATAGACCGG GTGAAGAGGA TTATATGGGA GAATCCACGA 
TTTTTGATCA GCAGCGGTGA TACGCCCACC AGTTTGAAGG AGGGCTGTCG CTATAATGCC 
ATGCACATCT GCGCCCAGGT CAATAAGGCC AGGATCGCTC AGTTGCTGTT AAAGACCATT 
TCGGATCGGG AGTTCACTCA GCTTTACGTT GGCAAGAAGG GCAGTGGCAA GATGTGTGCT 
GCCCTCAACA TCAGTCTCCT GGACTATTAC CTGAACATGC CGGACAAGGG GCGCGGCGAA 
ACACCGCTCC ACTTTGCCGC AAAGAACGGT CATGTGGCCA TGGTCGAGGT TCTCGTTTCC 
TATCCGGAGT GCAAATCGCT GCGGAATCAT GAGGGCAAGG AGCCCAAGGA AATCATCTGC 
CTGCGTAATG CTAATGCTAC ACATGTGACC ATCAAGAAGC TGGAGCTGCT CTTGTACGAT 
CCGCATTTTG TGCCCGTACT AAGATCCCAG TCAAATACAC TGCCGCCAAA AGTGGGTCAA 
CCGTTCTCGC CCAAAGATCC ACCGAACCTG CAACACAAAG CGGACGATTA CGAGGGCCTC 
AGCGTGGACC TGGCAATCAG TGCGCTGGCG GGACCCATGT CCCGCGAAAA GGCCATGAAC 
TTCTATCGCC GTTGGAAGAC ACCACCGCGG GTCAGCAACA ATGTGATGTC GCCGCTGGCT 
GGTTCACCAT TTAGCTCGCC GGTGAAAGTA ACCCCAAGCA AGTCGATCTT TGACCGAAGT 
GCTGGAAACT CGAGTCCAGT CCACTCAGGA CGCAGAGTGC TCTTTAGTCC ATTGGCGGAG 
GCGACCAGCT CACCAAAACC GACGAAAAAC GTGCCCAATG GCACCAATGA GTGCGAGCAC 
AACAATAATA ATGTGAAGCC AGTGTATCCG TTGGAGTTCC CGGCGACACC CATTCGAAAA 
ATGAAACCGG ATTTATTCAT GGCCTATCGC AATAACAATA GCTTTGATTC GCCATCTTTG 
GCCGATGACT CCCAAATCCT GGACATGAGC CTAAGCCGCA GCCTGAATGC GTCGCTAAAT 
GACAGCTTCC GTGAGCGGCA CATCAAGAAC ACTGATATCG AGAAGGGTCT GGAGGTGGTC 
GGCCGCCAAC TGGCACGACA GGAGCAGTTA GAGTGGCGCG AGTACTGGGA TTTTCTCGAT 
TCATTTTTGG ACATTGGTAC GACCGAAGGC CTGGCCCGTC TTGAAGCGTA TTTCCTGGAA 
AAGACCGAAC AGCAGGCGGA TAAATCAGAA ACGGTCTGGA ACTTTGCCCA TCTGCATCAG 
TATTTCGATT CGATGGCCGG CGAGCAACAG CAGCAACTCC GAAAGGATAA AAATGAGGCT 
GCGGGAGCAA CTTCGCCATC CGCCGGAGTC ATGACTCCGT ACACATGCGT AGAGAAGTCG 
CTGCAAGTGT TCGCCAAGCG CATCACTAAA ACGTTGATCA ACAAAATCGG CAACATGGTG 
TCCATCAACG ACACGCTGCT CTGTGAGCTC AAAAGACTGA AATCGCTGAT TGTCAGCTTC 
AAGGATGATG CCCGCTTCAT TAGCGTGGAC TTTAGCAAGG TGCATTCACG TATCGCCCAC 
CTGGTGGCCA GCTATGTGAC CCACTCGCAG GAGGTCAGCG TAGCCATGCG TCTACAATTG 
TTGCAGATGC TCCGAAGTTT GCGGCAACTG CTGGCCGACG AGCGTGGTCG AGAACAGCAT 
TTGGGCTGCG TGTGCGCTAG TCTATTGCTG ATGCTGGAAC AGGCGCCGAC ATCCGCCGTG 
CATCTACCAG ACACTCTGAA GACCGAGGAG CTATGTTGCG CCGCCTGGGA GACGGAGCAG 
TGTTGCGCCT GTCTGTGGGA CGCAAATCTC AGCCGTAAGA CCAGTCGTCG AAAGCGCACT 
AAGTCGCTGC GGGCAGCTGC TGTTGTTCAG TCTCAGGGTC AGCTTCAGGA TACTTCGGGA 
TCGACAGGGT CGTCCGCCTT GCACGCTTCG CTTGGTGTGG GATCGACCAG TTTGGGAGCA 
TCGAGGGTCG TGGCGTCCGC TTCGAAAGAT GCTTGGCGCC GTCAACAAAG CGACGACGAG 
GACTACGACA GCGATGAGCA AGTAATCTTT TTCGACTGCA CTAATGTTAC GCTGCCTTAT 
GGAAGCAGCA GCGAGGACGA GGAAAACTTC CGTACGCCGC CGCAAAGCTT GTCGCCAGGT 
ATTTCCATGG ATTTGGAGCC GCGTTACGAG TTGTTTATTT TTGGAAACGA GCCAACCAAG 
CGAGATTTGG ATGTGCTGAA TGCCCTTTCC AATGTCGACA TTGATAAGGA AACACTGCCG 
CATGTCTACG CCTGGAAGAC TGCCATGGAG AGCTACTCCT GTGCTGAAAT GAATCTCTTC 
CCATCCCCAA GGAACGTCAA GGTTCAAAAG CCGGAGCCTT GGTATTCTGG AACCAGTTCT 
AGCCACAACA GCCAACCATT GTTGCATCCC AAGCGTCTGC TTGCCACGCC AAAGCTGAAT 
GCCGTGGTCA GCGGCAGACG CGGATCCGGA CCATTGACGG CGCCAGTTAC ACCGCGTCTG 
GCGCGAACTC CGTCCGCCGC CAGTATTCAA GTTGCATCCG AGACGAATGG CGAGTCGGTC 
GGAACTGCTG TGACTCCGGC ATCGCCGATT TTGAGTTTTG CCGCCTTGAC GGCAGCGACG 
CAGTCATTCC AAACACCATT GAACAAGGTG CGCGGCTTGT TCAGCCAATA TCGGGATCAA 
CGGTCCTATA ACGAGGGGGA CACGCCGCTG GGCAATCGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573221.2
CDS	149..3673
Translation	MSTYFGVYIPTSKAGCFEGSVSQCIGSIAAVNIKPSNPASGSASVASGSPSGSAASVQTGNADDGSAATKYEDPDYPPDSPLWLIFTEKSKALDILRHYKEARLREFPNLEQAESYVQFGFESIEALKRFCKAKPESKPIPIISGSGYKSSPTSTDNSCSSSPTGNGSGFIIPLGSNSSMSNLLLSDSPTSSPSSSSNVIANGRQQQMQQQQQQQPQQPDVSGEGPPFRAPTKQELVEFRKQIEGGHIDRVKRIIWENPRFLISSGDTPTSLKEGCRYNAMHICAQVNKARIAQLLLKTISDREFTQLYVGKKGSGKMCAALNISLLDYYLNMPDKGRGETPLHFAAKNGHVAMVEVLVSYPECKSLRNHEGKEPKEIICLRNANATHVTIKKLELLLYDPHFVPVLRSQSNTLPPKVGQPFSPKDPPNLQHKADDYEGLSVDLAISALAGPMSREKAMNFYRRWKTPPRVSNNVMSPLAGSPFSSPVKVTPSKSIFDRSAGNSSPVHSGRRVLFSPLAEATSSPKPTKNVPNGTNECEHNNNNVKPVYPLEFPATPIRKMKPDLFMAYRNNNSFDSPSLADDSQILDMSLSRSLNASLNDSFRERHIKNTDIEKGLEVVGRQLARQEQLEWREYWDFLDSFLDIGTTEGLARLEAYFLEKTEQQADKSETVWNFAHLHQYFDSMAGEQQQQLRKDKNEAAGATSPSAGVMTPYTCVEKSLQVFAKRITKTLINKIGNMVSINDTLLCELKRLKSLIVSFKDDARFISVDFSKVHSRIAHLVASYVTHSQEVSVAMRLQLLQMLRSLRQLLADERGREQHLGCVCASLLLMLEQAPTSAVHLPDTLKTEELCCAAWETEQCCACLWDANLSRKTSRRKRTKSLRAAAVVQSQGQLQDTSGSTGSSALHASLGVGSTSLGASRVVASASKDAWRRQQSDDEDYDSDEQVIFFDCTNVTLPYGSSSEDEENFRTPPQSLSPGISMDLEPRYELFIFGNEPTKRDLDVLNALSNVDIDKETLPHVYAWKTAMESYSCAEMNLFPSPRNVKVQKPEPWYSGTSSSHNSQPLLHPKRLLATPKLNAVVSGRRGSGPLTAPVTPRLARTPSAASIQVASETNGESVGTAVTPASPILSFAALTAATQSFQTPLNKVRGLFSQYRDQRSYNEGDTPLGNRN

Target ORF information:

RefSeq Version	NM_132993.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant D (Ankle2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132993.3

ORF Insert Sequence:

ATGTCGACCT ATTTCGGGGT CTATATCCCG ACCTCCAAAG CGGGCTGTTT TGAGGGATCG 
GTGTCGCAGT GCATCGGCTC CATAGCCGCG GTGAACATAA AGCCATCCAA TCCGGCGTCT 
GGATCGGCAT CAGTAGCATC GGGATCGCCA TCCGGCTCGG CGGCATCCGT GCAAACGGGC 
AACGCAGACG ATGGCAGTGC TGCCACCAAG TACGAGGATC CCGACTATCC ACCGGACTCG 
CCACTGTGGC TGATCTTCAC GGAGAAATCC AAGGCGCTGG ACATCCTGCG ACACTACAAG 
GAGGCGCGCC TCCGCGAGTT TCCCAATCTG GAGCAGGCGG AGAGTTACGT TCAGTTTGGG 
TTCGAGAGCA TCGAGGCGCT CAAGAGATTT TGCAAGGCAA AGCCCGAAAG CAAGCCCATT 
CCGATAATCA GCGGTAGCGG TTACAAGAGC TCACCGACCT CGACGGACAA TTCGTGCTCC 
TCCTCGCCGA CGGGTAACGG CAGTGGCTTC ATCATTCCCC TGGGAAGCAA TTCCTCAATG 
TCGAATTTAC TGCTCAGTGA CTCACCGACT TCCTCGCCGA GCAGCTCCAG CAACGTCATT 
GCCAATGGGC GACAGCAGCA GATGCAGCAG CAACAGCAGC AGCAGCCGCA GCAGCCGGAT 
GTGTCCGGAG AAGGCCCTCC TTTCCGGGCG CCCACCAAAC AGGAACTGGT AGAGTTTCGC 
AAGCAAATCG AAGGTGGTCA CATAGACCGG GTGAAGAGGA TTATATGGGA GAATCCACGA 
TTTTTGATCA GCAGCGGTGA TACGCCCACC AGTTTGAAGG AGGGCTGTCG CTATAATGCC 
ATGCACATCT GCGCCCAGGT CAATAAGGCC AGGATCGCTC AGTTGCTGTT AAAGACCATT 
TCGGATCGGG AGTTCACTCA GCTTTACGTT GGCAAGAAGG GCAGTGGCAA GATGTGTGCT 
GCCCTCAACA TCAGTCTCCT GGACTATTAC CTGAACATGC CGGACAAGGG GCGCGGCGAA 
ACACCGCTCC ACTTTGCCGC AAAGAACGGT CATGTGGCCA TGGTCGAGGT TCTCGTTTCC 
TATCCGGAGT GCAAATCGCT GCGGAATCAT GAGGGCAAGG AGCCCAAGGA AATCATCTGC 
CTGCGTAATG CTAATGCTAC ACATGTGACC ATCAAGAAGC TGGAGCTGCT CTTGTACGAT 
CCGCATTTTG TGCCCGTACT AAGATCCCAG TCAAATACAC TGCCGCCAAA AGTGGGTCAA 
CCGTTCTCGC CCAAAGATCC ACCGAACCTG CAACACAAAG CGGACGATTA CGAGGGCCTC 
AGCGTGGACC TGGCAATCAG TGCGCTGGCG GGACCCATGT CCCGCGAAAA GGCCATGAAC 
TTCTATCGCC GTTGGAAGAC ACCACCGCGG GTCAGCAACA ATGTGATGTC GCCGCTGGCT 
GGTTCACCAT TTAGCTCGCC GGTGAAAGTA ACCCCAAGCA AGTCGATCTT TGACCGAAGT 
GCTGGAAACT CGAGTCCAGT CCACTCAGGA CGCAGAGTGC TCTTTAGTCC ATTGGCGGAG 
GCGACCAGCT CACCAAAACC GACGAAAAAC GTGCCCAATG GCACCAATGA GTGCGAGCAC 
AACAATAATA ATGTGAAGCC AGTGTATCCG TTGGAGTTCC CGGCGACACC CATTCGAAAA 
ATGAAACCGG ATTTATTCAT GGCCTATCGC AATAACAATA GCTTTGATTC GCCATCTTTG 
GCCGATGACT CCCAAATCCT GGACATGAGC CTAAGCCGCA GCCTGAATGC GTCGCTAAAT 
GACAGCTTCC GTGAGCGGCA CATCAAGAAC ACTGATATCG AGAAGGGTCT GGAGGTGGTC 
GGCCGCCAAC TGGCACGACA GGAGCAGTTA GAGTGGCGCG AGTACTGGGA TTTTCTCGAT 
TCATTTTTGG ACATTGGTAC GACCGAAGGC CTGGCCCGTC TTGAAGCGTA TTTCCTGGAA 
AAGACCGAAC AGCAGGCGGA TAAATCAGAA ACGGTCTGGA ACTTTGCCCA TCTGCATCAG 
TATTTCGATT CGATGGCCGG CGAGCAACAG CAGCAACTCC GAAAGGATAA AAATGAGGCT 
GCGGGAGCAA CTTCGCCATC CGCCGGAGTC ATGACTCCGT ACACATGCGT AGAGAAGTCG 
CTGCAAGTGT TCGCCAAGCG CATCACTAAA ACGTTGATCA ACAAAATCGG CAACATGGTG 
TCCATCAACG ACACGCTGCT CTGTGAGCTC AAAAGACTGA AATCGCTGAT TGTCAGCTTC 
AAGGATGATG CCCGCTTCAT TAGCGTGGAC TTTAGCAAGG TGCATTCACG TATCGCCCAC 
CTGGTGGCCA GCTATGTGAC CCACTCGCAG GAGGTCAGCG TAGCCATGCG TCTACAATTG 
TTGCAGATGC TCCGAAGTTT GCGGCAACTG CTGGCCGACG AGCGTGGTCG AGAACAGCAT 
TTGGGCTGCG TGTGCGCTAG TCTATTGCTG ATGCTGGAAC AGGCGCCGAC ATCCGCCGTG 
CATCTACCAG ACACTCTGAA GACCGAGGAG CTATGTTGCG CCGCCTGGGA GACGGAGCAG 
TGTTGCGCCT GTCTGTGGGA CGCAAATCTC AGCCGTAAGA CCAGTCGTCG AAAGCGCACT 
AAGTCGCTGC GGGCAGCTGC TGTTGTTCAG TCTCAGGGTC AGCTTCAGGA TACTTCGGGA 
TCGACAGGGT CGTCCGCCTT GCACGCTTCG CTTGGTGTGG GATCGACCAG TTTGGGAGCA 
TCGAGGGTCG TGGCGTCCGC TTCGAAAGAT GCTTGGCGCC GTCAACAAAG CGACGACGAG 
GACTACGACA GCGATGAGCA AGTAATCTTT TTCGACTGCA CTAATGTTAC GCTGCCTTAT 
GGAAGCAGCA GCGAGGACGA GGAAAACTTC CGTACGCCGC CGCAAAGCTT GTCGCCAGGT 
ATTTCCATGG ATTTGGAGCC GCGTTACGAG TTGTTTATTT TTGGAAACGA GCCAACCAAG 
CGAGATTTGG ATGTGCTGAA TGCCCTTTCC AATGTCGACA TTGATAAGGA AACACTGCCG 
CATGTCTACG CCTGGAAGAC TGCCATGGAG AGCTACTCCT GTGCTGAAAT GAATCTCTTC 
CCATCCCCAA GGAACGTCAA GGTTCAAAAG CCGGAGCCTT GGTATTCTGG AACCAGTTCT 
AGCCACAACA GCCAACCATT GTTGCATCCC AAGCGTCTGC TTGCCACGCC AAAGCTGAAT 
GCCGTGGTCA GCGGCAGACG CGGATCCGGA CCATTGACGG CGCCAGTTAC ACCGCGTCTG 
GCGCGAACTC CGTCCGCCGC CAGTATTCAA GTTGCATCCG AGACGAATGG CGAGTCGGTC 
GGAACTGCTG TGACTCCGGC ATCGCCGATT TTGAGTTTTG CCGCCTTGAC GGCAGCGACG 
CAGTCATTCC AAACACCATT GAACAAGGTG CGCGGCTTGT TCAGCCAATA TCGGGATCAA 
CGGTCCTATA ACGAGGGGGA CACGCCGCTG GGCAATCGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankle2 ( NM_167570.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728083.2 Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant F (Ankle2), mRNA.

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CloneID	OFa01914
Clone ID Related Accession (Same CDS sequence)	NM_167570.2
Accession Version	NM_167570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ankyrin repeat and LEM domain containing 2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_167570.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCACAC ATCAACATTG CCATCGCCAC GACGGAGCAG CGGACTCGCC ACTGTGGCTG 
ATCTTCACGG AGAAATCCAA GGCGCTGGAC ATCCTGCGAC ACTACAAGGA GGCGCGCCTC 
CGCGAGTTTC CCAATCTGGA GCAGGCGGAG AGTTACGTTC AGTTTGGGTT CGAGAGCATC 
GAGGCGCTCA AGAGATTTTG CAAGGCAAAG CCCGAAAGCA AGCCCATTCC GATAATCAGC 
GGTAGCGGTT ACAAGAGCTC ACCGACCTCG ACGGACAATT CGTGCTCCTC CTCGCCGACG 
GGTAACGGCA GTGGCTTCAT CATTCCCCTG GGAAGCAATT CCTCAATGTC GAATTTACTG 
CTCAGTGACT CACCGACTTC CTCGCCGAGC AGCTCCAGCA ACGTCATTGC CAATGGGCGA 
CAGCAGCAGA TGCAGCAGCA ACAGCAGCAG CAGCCGCAGC AGCCGGATGT GTCCGGAGAA 
GGCCCTCCTT TCCGGGCGCC CACCAAACAG GAACTGGTAG AGTTTCGCAA GCAAATCGAA 
GGTGGTCACA TAGACCGGGT GAAGAGGATT ATATGGGAGA ATCCACGATT TTTGATCAGC 
AGCGGTGATA CGCCCACCAG TTTGAAGGAG GGCTGTCGCT ATAATGCCAT GCACATCTGC 
GCCCAGGTCA ATAAGGCCAG GATCGCTCAG TTGCTGTTAA AGACCATTTC GGATCGGGAG 
TTCACTCAGC TTTACGTTGG CAAGAAGGGC AGTGGCAAGA TGTGTGCTGC CCTCAACATC 
AGTCTCCTGG ACTATTACCT GAACATGCCG GACAAGGGGC GCGGCGAAAC ACCGCTCCAC 
TTTGCCGCAA AGAACGGTCA TGTGGCCATG GTCGAGGTTC TCGTTTCCTA TCCGGAGTGC 
AAATCGCTGC GGAATCATGA GGGCAAGGAG CCCAAGGAAA TCATCTGCCT GCGTAATGCT 
AATGCTACAC ATGTGACCAT CAAGAAGCTG GAGCTGCTCT TGTACGATCC GCATTTTGTG 
CCCGTACTAA GATCCCAGTC AAATACACTG CCGCCAAAAG TGGGTCAACC GTTCTCGCCC 
AAAGATCCAC CGAACCTGCA ACACAAAGCG GACGATTACG AGGGCCTCAG CGTGGACCTG 
GCAATCAGTG CGCTGGCGGG ACCCATGTCC CGCGAAAAGG CCATGAACTT CTATCGCCGT 
TGGAAGACAC CACCGCGGGT CAGCAACAAT GTGATGTCGC CGCTGGCTGG TTCACCATTT 
AGCTCGCCGG TGAAAGTAAC CCCAAGCAAG TCGATCTTTG ACCGAAGTGC TGGAAACTCG 
AGTCCAGTCC ACTCAGGACG CAGAGTGCTC TTTAGTCCAT TGGCGGAGGC GACCAGCTCA 
CCAAAACCGA CGAAAAACGT GCCCAATGGC ACCAATGAGT GCGAGCACAA CAATAATAAT 
GTGAAGCCAG TGTATCCGTT GGAGTTCCCG GCGACACCCA TTCGAAAAAT GAAACCGGAT 
TTATTCATGG CCTATCGCAA TAACAATAGC TTTGATTCGC CATCTTTGGC CGATGACTCC 
CAAATCCTGG ACATGAGCCT AAGCCGCAGC CTGAATGCGT CGCTAAATGA CAGCTTCCGT 
GAGCGGCACA TCAAGAACAC TGATATCGAG AAGGGTCTGG AGGTGGTCGG CCGCCAACTG 
GCACGACAGG AGCAGTTAGA GTGGCGCGAG TACTGGGATT TTCTCGATTC ATTTTTGGAC 
ATTGGTACGA CCGAAGGCCT GGCCCGTCTT GAAGCGTATT TCCTGGAAAA GACCGAACAG 
CAGGCGGATA AATCAGAAAC GGTCTGGAAC TTTGCCCATC TGCATCAGTA TTTCGATTCG 
ATGGCCGGCG AGCAACAGCA GCAACTCCGA AAGGATAAAA ATGAGGCTGC GGGAGCAACT 
TCGCCATCCG CCGGAGTCAT GACTCCGTAC ACATGCGTAG AGAAGTCGCT GCAAGTGTTC 
GCCAAGCGCA TCACTAAAAC GTTGATCAAC AAAATCGGCA ACATGGTGTC CATCAACGAC 
ACGCTGCTCT GTGAGCTCAA AAGACTGAAA TCGCTGATTG TCAGCTTCAA GGATGATGCC 
CGCTTCATTA GCGTGGACTT TAGCAAGGTG CATTCACGTA TCGCCCACCT GGTGGCCAGC 
TATGTGACCC ACTCGCAGGA GGTCAGCGTA GCCATGCGTC TACAATTGTT GCAGATGCTC 
CGAAGTTTGC GGCAACTGCT GGCCGACGAG CGTGGTCGAG AACAGCATTT GGGCTGCGTG 
TGCGCTAGTC TATTGCTGAT GCTGGAACAG GCGCCGACAT CCGCCGTGCA TCTACCAGAC 
ACTCTGAAGA CCGAGGAGCT ATGTTGCGCC GCCTGGGAGA CGGAGCAGTG TTGCGCCTGT 
CTGTGGGACG CAAATCTCAG CCGTAAGACC AGTCGTCGAA AGCGCACTAA GTCGCTGCGG 
GCAGCTGCTG TTGTTCAGTC TCAGGGTCAG CTTCAGGATA CTTCGGGATC GACAGGGTCG 
TCCGCCTTGC ACGCTTCGCT TGGTGTGGGA TCGACCAGTT TGGGAGCATC GAGGGTCGTG 
GCGTCCGCTT CGAAAGATGC TTGGCGCCGT CAACAAAGCG ACGACGAGGA CTACGACAGC 
GATGAGCAAG TAATCTTTTT CGACTGCACT AATGTTACGC TGCCTTATGG AAGCAGCAGC 
GAGGACGAGG AAAACTTCCG TACGCCGCCG CAAAGCTTGT CGCCAGGTAT TTCCATGGAT 
TTGGAGCCGC GTTACGAGTT GTTTATTTTT GGAAACGAGC CAACCAAGCG AGATTTGGAT 
GTGCTGAATG CCCTTTCCAA TGTCGACATT GATAAGGAAA CACTGCCGCA TGTCTACGCC 
TGGAAGACTG CCATGGAGAG CTACTCCTGT GCTGAAATGA ATCTCTTCCC ATCCCCAAGG 
AACGTCAAGG TTCAAAAGCC GGAGCCTTGG TATTCTGGAA CCAGTTCTAG CCACAACAGC 
CAACCATTGT TGCATCCCAA GCGTCTGCTT GCCACGCCAA AGCTGAATGC CGTGGTCAGC 
GGCAGACGCG GATCCGGACC ATTGACGGCG CCAGTTACAC CGCGTCTGGC GCGAACTCCG 
TCCGCCGCCA GTATTCAAGT TGCATCCGAG ACGAATGGCG AGTCGGTCGG AACTGCTGTG 
ACTCCGGCAT CGCCGATTTT GAGTTTTGCC GCCTTGACGG CAGCGACGCA GTCATTCCAA 
ACACCATTGA ACAAGGTGCG CGGCTTGTTC AGCCAATATC GGGATCAACG GTCCTATAAC 
GAGGGGGACA CGCCGCTGGG CAATCGGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728083.2
CDS	40..3372
Translation	MPTHQHCHRHDGAADSPLWLIFTEKSKALDILRHYKEARLREFPNLEQAESYVQFGFESIEALKRFCKAKPESKPIPIISGSGYKSSPTSTDNSCSSSPTGNGSGFIIPLGSNSSMSNLLLSDSPTSSPSSSSNVIANGRQQQMQQQQQQQPQQPDVSGEGPPFRAPTKQELVEFRKQIEGGHIDRVKRIIWENPRFLISSGDTPTSLKEGCRYNAMHICAQVNKARIAQLLLKTISDREFTQLYVGKKGSGKMCAALNISLLDYYLNMPDKGRGETPLHFAAKNGHVAMVEVLVSYPECKSLRNHEGKEPKEIICLRNANATHVTIKKLELLLYDPHFVPVLRSQSNTLPPKVGQPFSPKDPPNLQHKADDYEGLSVDLAISALAGPMSREKAMNFYRRWKTPPRVSNNVMSPLAGSPFSSPVKVTPSKSIFDRSAGNSSPVHSGRRVLFSPLAEATSSPKPTKNVPNGTNECEHNNNNVKPVYPLEFPATPIRKMKPDLFMAYRNNNSFDSPSLADDSQILDMSLSRSLNASLNDSFRERHIKNTDIEKGLEVVGRQLARQEQLEWREYWDFLDSFLDIGTTEGLARLEAYFLEKTEQQADKSETVWNFAHLHQYFDSMAGEQQQQLRKDKNEAAGATSPSAGVMTPYTCVEKSLQVFAKRITKTLINKIGNMVSINDTLLCELKRLKSLIVSFKDDARFISVDFSKVHSRIAHLVASYVTHSQEVSVAMRLQLLQMLRSLRQLLADERGREQHLGCVCASLLLMLEQAPTSAVHLPDTLKTEELCCAAWETEQCCACLWDANLSRKTSRRKRTKSLRAAAVVQSQGQLQDTSGSTGSSALHASLGVGSTSLGASRVVASASKDAWRRQQSDDEDYDSDEQVIFFDCTNVTLPYGSSSEDEENFRTPPQSLSPGISMDLEPRYELFIFGNEPTKRDLDVLNALSNVDIDKETLPHVYAWKTAMESYSCAEMNLFPSPRNVKVQKPEPWYSGTSSSHNSQPLLHPKRLLATPKLNAVVSGRRGSGPLTAPVTPRLARTPSAASIQVASETNGESVGTAVTPASPILSFAALTAATQSFQTPLNKVRGLFSQYRDQRSYNEGDTPLGNRN

Target ORF information:

RefSeq Version	NM_167570.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant F (Ankle2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167570.2

ORF Insert Sequence:

ATGCCCACAC ATCAACATTG CCATCGCCAC GACGGAGCAG CGGACTCGCC ACTGTGGCTG 
ATCTTCACGG AGAAATCCAA GGCGCTGGAC ATCCTGCGAC ACTACAAGGA GGCGCGCCTC 
CGCGAGTTTC CCAATCTGGA GCAGGCGGAG AGTTACGTTC AGTTTGGGTT CGAGAGCATC 
GAGGCGCTCA AGAGATTTTG CAAGGCAAAG CCCGAAAGCA AGCCCATTCC GATAATCAGC 
GGTAGCGGTT ACAAGAGCTC ACCGACCTCG ACGGACAATT CGTGCTCCTC CTCGCCGACG 
GGTAACGGCA GTGGCTTCAT CATTCCCCTG GGAAGCAATT CCTCAATGTC GAATTTACTG 
CTCAGTGACT CACCGACTTC CTCGCCGAGC AGCTCCAGCA ACGTCATTGC CAATGGGCGA 
CAGCAGCAGA TGCAGCAGCA ACAGCAGCAG CAGCCGCAGC AGCCGGATGT GTCCGGAGAA 
GGCCCTCCTT TCCGGGCGCC CACCAAACAG GAACTGGTAG AGTTTCGCAA GCAAATCGAA 
GGTGGTCACA TAGACCGGGT GAAGAGGATT ATATGGGAGA ATCCACGATT TTTGATCAGC 
AGCGGTGATA CGCCCACCAG TTTGAAGGAG GGCTGTCGCT ATAATGCCAT GCACATCTGC 
GCCCAGGTCA ATAAGGCCAG GATCGCTCAG TTGCTGTTAA AGACCATTTC GGATCGGGAG 
TTCACTCAGC TTTACGTTGG CAAGAAGGGC AGTGGCAAGA TGTGTGCTGC CCTCAACATC 
AGTCTCCTGG ACTATTACCT GAACATGCCG GACAAGGGGC GCGGCGAAAC ACCGCTCCAC 
TTTGCCGCAA AGAACGGTCA TGTGGCCATG GTCGAGGTTC TCGTTTCCTA TCCGGAGTGC 
AAATCGCTGC GGAATCATGA GGGCAAGGAG CCCAAGGAAA TCATCTGCCT GCGTAATGCT 
AATGCTACAC ATGTGACCAT CAAGAAGCTG GAGCTGCTCT TGTACGATCC GCATTTTGTG 
CCCGTACTAA GATCCCAGTC AAATACACTG CCGCCAAAAG TGGGTCAACC GTTCTCGCCC 
AAAGATCCAC CGAACCTGCA ACACAAAGCG GACGATTACG AGGGCCTCAG CGTGGACCTG 
GCAATCAGTG CGCTGGCGGG ACCCATGTCC CGCGAAAAGG CCATGAACTT CTATCGCCGT 
TGGAAGACAC CACCGCGGGT CAGCAACAAT GTGATGTCGC CGCTGGCTGG TTCACCATTT 
AGCTCGCCGG TGAAAGTAAC CCCAAGCAAG TCGATCTTTG ACCGAAGTGC TGGAAACTCG 
AGTCCAGTCC ACTCAGGACG CAGAGTGCTC TTTAGTCCAT TGGCGGAGGC GACCAGCTCA 
CCAAAACCGA CGAAAAACGT GCCCAATGGC ACCAATGAGT GCGAGCACAA CAATAATAAT 
GTGAAGCCAG TGTATCCGTT GGAGTTCCCG GCGACACCCA TTCGAAAAAT GAAACCGGAT 
TTATTCATGG CCTATCGCAA TAACAATAGC TTTGATTCGC CATCTTTGGC CGATGACTCC 
CAAATCCTGG ACATGAGCCT AAGCCGCAGC CTGAATGCGT CGCTAAATGA CAGCTTCCGT 
GAGCGGCACA TCAAGAACAC TGATATCGAG AAGGGTCTGG AGGTGGTCGG CCGCCAACTG 
GCACGACAGG AGCAGTTAGA GTGGCGCGAG TACTGGGATT TTCTCGATTC ATTTTTGGAC 
ATTGGTACGA CCGAAGGCCT GGCCCGTCTT GAAGCGTATT TCCTGGAAAA GACCGAACAG 
CAGGCGGATA AATCAGAAAC GGTCTGGAAC TTTGCCCATC TGCATCAGTA TTTCGATTCG 
ATGGCCGGCG AGCAACAGCA GCAACTCCGA AAGGATAAAA ATGAGGCTGC GGGAGCAACT 
TCGCCATCCG CCGGAGTCAT GACTCCGTAC ACATGCGTAG AGAAGTCGCT GCAAGTGTTC 
GCCAAGCGCA TCACTAAAAC GTTGATCAAC AAAATCGGCA ACATGGTGTC CATCAACGAC 
ACGCTGCTCT GTGAGCTCAA AAGACTGAAA TCGCTGATTG TCAGCTTCAA GGATGATGCC 
CGCTTCATTA GCGTGGACTT TAGCAAGGTG CATTCACGTA TCGCCCACCT GGTGGCCAGC 
TATGTGACCC ACTCGCAGGA GGTCAGCGTA GCCATGCGTC TACAATTGTT GCAGATGCTC 
CGAAGTTTGC GGCAACTGCT GGCCGACGAG CGTGGTCGAG AACAGCATTT GGGCTGCGTG 
TGCGCTAGTC TATTGCTGAT GCTGGAACAG GCGCCGACAT CCGCCGTGCA TCTACCAGAC 
ACTCTGAAGA CCGAGGAGCT ATGTTGCGCC GCCTGGGAGA CGGAGCAGTG TTGCGCCTGT 
CTGTGGGACG CAAATCTCAG CCGTAAGACC AGTCGTCGAA AGCGCACTAA GTCGCTGCGG 
GCAGCTGCTG TTGTTCAGTC TCAGGGTCAG CTTCAGGATA CTTCGGGATC GACAGGGTCG 
TCCGCCTTGC ACGCTTCGCT TGGTGTGGGA TCGACCAGTT TGGGAGCATC GAGGGTCGTG 
GCGTCCGCTT CGAAAGATGC TTGGCGCCGT CAACAAAGCG ACGACGAGGA CTACGACAGC 
GATGAGCAAG TAATCTTTTT CGACTGCACT AATGTTACGC TGCCTTATGG AAGCAGCAGC 
GAGGACGAGG AAAACTTCCG TACGCCGCCG CAAAGCTTGT CGCCAGGTAT TTCCATGGAT 
TTGGAGCCGC GTTACGAGTT GTTTATTTTT GGAAACGAGC CAACCAAGCG AGATTTGGAT 
GTGCTGAATG CCCTTTCCAA TGTCGACATT GATAAGGAAA CACTGCCGCA TGTCTACGCC 
TGGAAGACTG CCATGGAGAG CTACTCCTGT GCTGAAATGA ATCTCTTCCC ATCCCCAAGG 
AACGTCAAGG TTCAAAAGCC GGAGCCTTGG TATTCTGGAA CCAGTTCTAG CCACAACAGC 
CAACCATTGT TGCATCCCAA GCGTCTGCTT GCCACGCCAA AGCTGAATGC CGTGGTCAGC 
GGCAGACGCG GATCCGGACC ATTGACGGCG CCAGTTACAC CGCGTCTGGC GCGAACTCCG 
TCCGCCGCCA GTATTCAAGT TGCATCCGAG ACGAATGGCG AGTCGGTCGG AACTGCTGTG 
ACTCCGGCAT CGCCGATTTT GAGTTTTGCC GCCTTGACGG CAGCGACGCA GTCATTCCAA 
ACACCATTGA ACAAGGTGCG CGGCTTGTTC AGCCAATATC GGGATCAACG GTCCTATAAC 
GAGGGGGACA CGCCGCTGGG CAATCGGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankle2 ( NM_001298446.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285375.1 Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant G (Ankle2), mRNA.

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CloneID	OFa21517
Clone ID Related Accession (Same CDS sequence)	NM_001298446.1
Accession Version	NM_001298446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ankyrin repeat and LEM domain containing 2, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACCT ATTTCGGGGT CTATATCCCG ACCTCCAAAG CGGGCTGTTT TGAGGGATCG 
GTGTCGCAGT GCATCGGCTC CATAGCCGCG GTGAACATAA AGCCATCCAA TCCGGCGTCT 
GGATCGGCAT CAGTAGCATC GGGATCGCCA TCCGGCTCGG CGGCATCCGT GCAAACGGGC 
AACGCAGACG ATGGCAGTGC TGCCACCAAG TACGAGGATC CCGACTATCC ACCGGACTCG 
CCACTGTGGC TGATCTTCAC GGAGAAATCC AAGGCGCTGG ACATCCTGCG ACACTACAAG 
GAGGCGCGCC TCCGCGAGTT TCCCAATCTG GAGCAGGCGG AGAGTTACGT TCAGTTTGGG 
TTCGAGAGCA TCGAGGCGCT CAAGAGATTT TGCAAGGCAA AGCCCGAAAG CAAGCCCATT 
CCGATAATCA GCGGTAGCGG TTACAAGAGC TCACCGACCT CGACGGACAA TTCGTGCTCC 
TCCTCGCCGA CGGGTAACGG CAGTGGCTTC ATCATTCCCC TGGGAAGCAA TTCCTCAATG 
TCGAATTTAC TGCTCAGTGA CTCACCGACT TCCTCGCCGA GCAGCTCCAG CAACGTCATT 
GCCAATGGGC GACAGCAGCA GATGCAGCAG CAACAGCAGC AGCAGCCGCA GCAGCCGGAT 
GTGTCCGGAG AAGGCCCTCC TTTCCGGGCG CCCACCAAAC AGGAACTGGT AGAGTTTCGC 
AAGCAAATCG AAGGTGGTCA CATAGACCGG GTGAAGAGGA TTATATGGGA GAATCCACGA 
TTTTTGATCA GCAGCGGTGA TACGCCCACC AGTTTGAAGG AGGGCTGTCG CTATAATGCC 
ATGCACATCT GCGCCCAGGT CAATAAGGCC AGGATCGCTC AGTTGCTGTT AAAGACCATT 
TCGGATCGGG AGTTCACTCA GCTTTACGTT GGCAAGAAGG GCAGTGGCAA GATGTGTGCT 
GCCCTCAACA TCAGTCTCCT GGACTATTAC CTGAACATGC CGGACAAGGG GCGCGGCGAA 
ACACCGCTCC ACTTTGCCGC AAAGAACGGT CATGTGGCCA TGGTCGAGGT TCTCGTTTCC 
TATCCGGAGT GCAAATCGCT GCGGAATCAT GAGGGCAAGG AGCCCAAGGA AATCATCTGC 
CTGCGTAATG CTAATGCTAC ACATGTGACC ATCAAGAAGC TGGAGCTGCT CTTGTACGAT 
CCGCATTTTG TGCCCGTACT AAGATCCCAG TCAAATACAC TGCCGCCAAA AGTGGGTCAA 
CCGTTCTCGC CCAAAGATCC ACCGAACCTG CAACACAAAG CGGACGATTA CGAGGGCCTC 
AGCGTGGACC TGGCAATCAG TGCGCTGGCG GGACCCATGT CCCGCGAAAA GGCCATGAAC 
TTCTATCGCC GTTGGAAGAC ACCACCGCGG GTCAGCAACA ATGTGATGTC GCCGCTGGCT 
GGTTCACCAT TTAGCTCGCC GGTGAAAGTA ACCCCAAGCA AGTCGATCTT TGACCGAAGT 
GCTGGAAACT CGAGTCCAGT CCACTCAGGA CGCAGAGTGC TCTTTAGTCC ATTGGCGGAG 
GCGACCAGCT CACCAAAACC GACGAAAAAC GTGCCCAATG GCACCAATGA GTGCGAGCAC 
AACAATAATA ATGTGAAGCC AGTGTATCCG TTGGAGTTCC CGGCGACACC CATTCGAAAA 
ATGAAACCGG ATTTATTCAT GGCCTATCGC AATAACAATA GCTTTGATTC GCCATCTTTG 
GCCGATGACT CCCAAATCCT GGACATGAGC CTAAGCCGCA GCCTGAATGC GTCGCTAAAT 
GACAGCTTCC GTGAGCGGCA CATCAAGAAC ACTGATATCG AGAAGGGTCT GGAGGTGGTC 
GGCCGCCAAC TGGCACGACA GGAGCAGTTA GAGTGGCGCG AGTACTGGGA TTTTCTCGAT 
TCATTTTTGG ACATTGGTAC GACCGAAGGC CTGGCCCGTC TTGAAGCGTA TTTCCTGGAA 
AAGACCGAAC AGCAGGCGGA TAAATCAGAA ACGGTCTGGA ACTTTGCCCA TCTGCATCAG 
TATTTCGATT CGATGGCCGG CGAGCAACAG CAGCAACTCC GAAAGGATAA AAATGAGGCT 
GCGGGAGCAA CTTCGCCATC CGCCGGAGTC ATGACTCCGT ACACATGCGT AGAGAAGTCG 
CTGCAAGTGT TCGCCAAGCG CATCACTAAA ACGTTGATCA ACAAAATCGG CAACATGGTG 
TCCATCAACG ACACGCTGCT CTGTGAGCTC AAAAGACTGA AATCGCTGAT TGTCAGCTTC 
AAGGATGATG CCCGCTTCAT TAGCGTGGAC TTTAGCAAGG TGCATTCACG TATCGCCCAC 
CTGGTGGCCA GCTATGTGAC CCACTCGCAG GAGGTCAGCG TAGCCATGCG TCTACAATTG 
TTGCAGATGC TCCGAAGTTT GCGGCAACTG CTGGCCGACG AGCGTGGTCG AGAACAGCAT 
TTGGGCTGCG TGTGCGCTAG TCTATTGCTG ATGCTGGAAC AGGCGCCGAC ATCCGCCGTG 
CATCTACCAG ACACTCTGAA GACCGAGGAG CTATGTTGCG CCGCCTGGGA GACGGAGCAG 
TGTTGCGCCT GTCTGTGGGA CGCAAATCTC AGCCGTAAGA CCAGTCGTCG AAAGCGCACT 
AAGTCGCTGC GGGCAGCTGC TGTTGTTCAG TCTCAGGGTC AGCTTCAGGA TACTTCGGGA 
TCGACAGGGT CGTCCGCCTT GCACGCTTCG CTTGGTGTGG GATCGACCAG TTTGGGAGCA 
TCGAGGGTCG TGGCGTCCGC TTCGAAAGAT GCTTGGCGCC GTCAACAAAG CGACGACGAG 
GACTACGACA GCGATGAGCA AGTAATCTTT TTCGACTGCA CTAATGTTAC GCTGCCTTAT 
GGAAGCAGCA GCGAGGACGA GGAAAACTTC CGTACGCCGC CGCAAAGCTT GTCGCCAGGT 
ATTTCCATGG ATTTGGAGCC GCGTTACGAG TTGTTTATTT TTGGAAACGA GCCAACCAAG 
CGAGATTTGG ATGTGCTGAA TGCCCTTTCC AATGTCGACA TTGATAAGGA AACACTGCCG 
CATGTCTACG CCTGGAAGAC TGCCATGGAG AGCTACTCCT GTGCTGAAAT GAATCTGAAC 
GTCAAGGTTC AAAAGCCGGA GCCTTGGTAT TCTGGAACCA GTTCTAGCCA CAACAGCCAA 
CCATTGTTGC ATCCCAAGCG TCTGCTTGCC ACGCCAAAGC TGAATGCCGT GGTCAGCGGC 
AGACGCGGAT CCGGACCATT GACGGCGCCA GTTACACCGC GTCTGGCGCG AACTCCGTCC 
GCCGCCAGTA TTCAAGTTGC ATCCGAGACG AATGGCGAGT CGGTCGGAAC TGCTGTGACT 
CCGGCATCGC CGATTTTGAG TTTTGCCGCC TTGACGGCAG CGACGCAGTC ATTCCAAACA 
CCATTGAACA AGGTGCGCGG CTTGTTCAGC CAATATCGGG ATCAACGGTC CTATAACGAG 
GGGGACACGC CGCTGGGCAA TCGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285375.1
CDS	149..3658
Translation	MSTYFGVYIPTSKAGCFEGSVSQCIGSIAAVNIKPSNPASGSASVASGSPSGSAASVQTGNADDGSAATKYEDPDYPPDSPLWLIFTEKSKALDILRHYKEARLREFPNLEQAESYVQFGFESIEALKRFCKAKPESKPIPIISGSGYKSSPTSTDNSCSSSPTGNGSGFIIPLGSNSSMSNLLLSDSPTSSPSSSSNVIANGRQQQMQQQQQQQPQQPDVSGEGPPFRAPTKQELVEFRKQIEGGHIDRVKRIIWENPRFLISSGDTPTSLKEGCRYNAMHICAQVNKARIAQLLLKTISDREFTQLYVGKKGSGKMCAALNISLLDYYLNMPDKGRGETPLHFAAKNGHVAMVEVLVSYPECKSLRNHEGKEPKEIICLRNANATHVTIKKLELLLYDPHFVPVLRSQSNTLPPKVGQPFSPKDPPNLQHKADDYEGLSVDLAISALAGPMSREKAMNFYRRWKTPPRVSNNVMSPLAGSPFSSPVKVTPSKSIFDRSAGNSSPVHSGRRVLFSPLAEATSSPKPTKNVPNGTNECEHNNNNVKPVYPLEFPATPIRKMKPDLFMAYRNNNSFDSPSLADDSQILDMSLSRSLNASLNDSFRERHIKNTDIEKGLEVVGRQLARQEQLEWREYWDFLDSFLDIGTTEGLARLEAYFLEKTEQQADKSETVWNFAHLHQYFDSMAGEQQQQLRKDKNEAAGATSPSAGVMTPYTCVEKSLQVFAKRITKTLINKIGNMVSINDTLLCELKRLKSLIVSFKDDARFISVDFSKVHSRIAHLVASYVTHSQEVSVAMRLQLLQMLRSLRQLLADERGREQHLGCVCASLLLMLEQAPTSAVHLPDTLKTEELCCAAWETEQCCACLWDANLSRKTSRRKRTKSLRAAAVVQSQGQLQDTSGSTGSSALHASLGVGSTSLGASRVVASASKDAWRRQQSDDEDYDSDEQVIFFDCTNVTLPYGSSSEDEENFRTPPQSLSPGISMDLEPRYELFIFGNEPTKRDLDVLNALSNVDIDKETLPHVYAWKTAMESYSCAEMNLNVKVQKPEPWYSGTSSSHNSQPLLHPKRLLATPKLNAVVSGRRGSGPLTAPVTPRLARTPSAASIQVASETNGESVGTAVTPASPILSFAALTAATQSFQTPLNKVRGLFSQYRDQRSYNEGDTPLGNRN

Target ORF information:

RefSeq Version	NM_001298446.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ankyrin repeat and LEM domain containing 2, transcript variant G (Ankle2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298446.1

ORF Insert Sequence:

ATGTCGACCT ATTTCGGGGT CTATATCCCG ACCTCCAAAG CGGGCTGTTT TGAGGGATCG 
GTGTCGCAGT GCATCGGCTC CATAGCCGCG GTGAACATAA AGCCATCCAA TCCGGCGTCT 
GGATCGGCAT CAGTAGCATC GGGATCGCCA TCCGGCTCGG CGGCATCCGT GCAAACGGGC 
AACGCAGACG ATGGCAGTGC TGCCACCAAG TACGAGGATC CCGACTATCC ACCGGACTCG 
CCACTGTGGC TGATCTTCAC GGAGAAATCC AAGGCGCTGG ACATCCTGCG ACACTACAAG 
GAGGCGCGCC TCCGCGAGTT TCCCAATCTG GAGCAGGCGG AGAGTTACGT TCAGTTTGGG 
TTCGAGAGCA TCGAGGCGCT CAAGAGATTT TGCAAGGCAA AGCCCGAAAG CAAGCCCATT 
CCGATAATCA GCGGTAGCGG TTACAAGAGC TCACCGACCT CGACGGACAA TTCGTGCTCC 
TCCTCGCCGA CGGGTAACGG CAGTGGCTTC ATCATTCCCC TGGGAAGCAA TTCCTCAATG 
TCGAATTTAC TGCTCAGTGA CTCACCGACT TCCTCGCCGA GCAGCTCCAG CAACGTCATT 
GCCAATGGGC GACAGCAGCA GATGCAGCAG CAACAGCAGC AGCAGCCGCA GCAGCCGGAT 
GTGTCCGGAG AAGGCCCTCC TTTCCGGGCG CCCACCAAAC AGGAACTGGT AGAGTTTCGC 
AAGCAAATCG AAGGTGGTCA CATAGACCGG GTGAAGAGGA TTATATGGGA GAATCCACGA 
TTTTTGATCA GCAGCGGTGA TACGCCCACC AGTTTGAAGG AGGGCTGTCG CTATAATGCC 
ATGCACATCT GCGCCCAGGT CAATAAGGCC AGGATCGCTC AGTTGCTGTT AAAGACCATT 
TCGGATCGGG AGTTCACTCA GCTTTACGTT GGCAAGAAGG GCAGTGGCAA GATGTGTGCT 
GCCCTCAACA TCAGTCTCCT GGACTATTAC CTGAACATGC CGGACAAGGG GCGCGGCGAA 
ACACCGCTCC ACTTTGCCGC AAAGAACGGT CATGTGGCCA TGGTCGAGGT TCTCGTTTCC 
TATCCGGAGT GCAAATCGCT GCGGAATCAT GAGGGCAAGG AGCCCAAGGA AATCATCTGC 
CTGCGTAATG CTAATGCTAC ACATGTGACC ATCAAGAAGC TGGAGCTGCT CTTGTACGAT 
CCGCATTTTG TGCCCGTACT AAGATCCCAG TCAAATACAC TGCCGCCAAA AGTGGGTCAA 
CCGTTCTCGC CCAAAGATCC ACCGAACCTG CAACACAAAG CGGACGATTA CGAGGGCCTC 
AGCGTGGACC TGGCAATCAG TGCGCTGGCG GGACCCATGT CCCGCGAAAA GGCCATGAAC 
TTCTATCGCC GTTGGAAGAC ACCACCGCGG GTCAGCAACA ATGTGATGTC GCCGCTGGCT 
GGTTCACCAT TTAGCTCGCC GGTGAAAGTA ACCCCAAGCA AGTCGATCTT TGACCGAAGT 
GCTGGAAACT CGAGTCCAGT CCACTCAGGA CGCAGAGTGC TCTTTAGTCC ATTGGCGGAG 
GCGACCAGCT CACCAAAACC GACGAAAAAC GTGCCCAATG GCACCAATGA GTGCGAGCAC 
AACAATAATA ATGTGAAGCC AGTGTATCCG TTGGAGTTCC CGGCGACACC CATTCGAAAA 
ATGAAACCGG ATTTATTCAT GGCCTATCGC AATAACAATA GCTTTGATTC GCCATCTTTG 
GCCGATGACT CCCAAATCCT GGACATGAGC CTAAGCCGCA GCCTGAATGC GTCGCTAAAT 
GACAGCTTCC GTGAGCGGCA CATCAAGAAC ACTGATATCG AGAAGGGTCT GGAGGTGGTC 
GGCCGCCAAC TGGCACGACA GGAGCAGTTA GAGTGGCGCG AGTACTGGGA TTTTCTCGAT 
TCATTTTTGG ACATTGGTAC GACCGAAGGC CTGGCCCGTC TTGAAGCGTA TTTCCTGGAA 
AAGACCGAAC AGCAGGCGGA TAAATCAGAA ACGGTCTGGA ACTTTGCCCA TCTGCATCAG 
TATTTCGATT CGATGGCCGG CGAGCAACAG CAGCAACTCC GAAAGGATAA AAATGAGGCT 
GCGGGAGCAA CTTCGCCATC CGCCGGAGTC ATGACTCCGT ACACATGCGT AGAGAAGTCG 
CTGCAAGTGT TCGCCAAGCG CATCACTAAA ACGTTGATCA ACAAAATCGG CAACATGGTG 
TCCATCAACG ACACGCTGCT CTGTGAGCTC AAAAGACTGA AATCGCTGAT TGTCAGCTTC 
AAGGATGATG CCCGCTTCAT TAGCGTGGAC TTTAGCAAGG TGCATTCACG TATCGCCCAC 
CTGGTGGCCA GCTATGTGAC CCACTCGCAG GAGGTCAGCG TAGCCATGCG TCTACAATTG 
TTGCAGATGC TCCGAAGTTT GCGGCAACTG CTGGCCGACG AGCGTGGTCG AGAACAGCAT 
TTGGGCTGCG TGTGCGCTAG TCTATTGCTG ATGCTGGAAC AGGCGCCGAC ATCCGCCGTG 
CATCTACCAG ACACTCTGAA GACCGAGGAG CTATGTTGCG CCGCCTGGGA GACGGAGCAG 
TGTTGCGCCT GTCTGTGGGA CGCAAATCTC AGCCGTAAGA CCAGTCGTCG AAAGCGCACT 
AAGTCGCTGC GGGCAGCTGC TGTTGTTCAG TCTCAGGGTC AGCTTCAGGA TACTTCGGGA 
TCGACAGGGT CGTCCGCCTT GCACGCTTCG CTTGGTGTGG GATCGACCAG TTTGGGAGCA 
TCGAGGGTCG TGGCGTCCGC TTCGAAAGAT GCTTGGCGCC GTCAACAAAG CGACGACGAG 
GACTACGACA GCGATGAGCA AGTAATCTTT TTCGACTGCA CTAATGTTAC GCTGCCTTAT 
GGAAGCAGCA GCGAGGACGA GGAAAACTTC CGTACGCCGC CGCAAAGCTT GTCGCCAGGT 
ATTTCCATGG ATTTGGAGCC GCGTTACGAG TTGTTTATTT TTGGAAACGA GCCAACCAAG 
CGAGATTTGG ATGTGCTGAA TGCCCTTTCC AATGTCGACA TTGATAAGGA AACACTGCCG 
CATGTCTACG CCTGGAAGAC TGCCATGGAG AGCTACTCCT GTGCTGAAAT GAATCTGAAC 
GTCAAGGTTC AAAAGCCGGA GCCTTGGTAT TCTGGAACCA GTTCTAGCCA CAACAGCCAA 
CCATTGTTGC ATCCCAAGCG TCTGCTTGCC ACGCCAAAGC TGAATGCCGT GGTCAGCGGC 
AGACGCGGAT CCGGACCATT GACGGCGCCA GTTACACCGC GTCTGGCGCG AACTCCGTCC 
GCCGCCAGTA TTCAAGTTGC ATCCGAGACG AATGGCGAGT CGGTCGGAAC TGCTGTGACT 
CCGGCATCGC CGATTTTGAG TTTTGCCGCC TTGACGGCAG CGACGCAGTC ATTCCAAACA 
CCATTGAACA AGGTGCGCGG CTTGTTCAGC CAATATCGGG ATCAACGGTC CTATAACGAG 
GGGGACACGC CGCTGGGCAA TCGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ankle2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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