Ubr1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ubr1
Entrez Gene ID	32687
Full Name	Ubr1 ubiquitin ligase
Synonyms	CG9086,Dmel\CG9086,UBR1,ubr1
General protein information	Preferred Names Ubr1 ubiquitin ligase Names CG9086 gene product from transcript CG9086-RC CG9086-PA CG9086-PB CG9086-PC Ubr1-PA Ubr1-PB Ubr1-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 15C4-15C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298427.1	NP_001285356.1	Ubr1 ubiquitin ligase, isoform C
NM_132956.3	NP_573184.1	Ubr1 ubiquitin ligase, isoform A
NM_001258794.2	NP_001245723.1	Ubr1 ubiquitin ligase, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ubr2	NP_001171542.1
Gallus gallus (chicken)	UBR2	XP_004935398.1
Drosophila melanogaster (fruit fly)	CG9086	NP_001245723.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000171	XP_310967.5
Caenorhabditis elegans (roundworm)	ubr-1	NP_491231.2
Xenopus tropicalis (tropical clawed frog)	ubr2	XP_002934736.1
	UBR2	XP_001088074.2
Bos taurus (cattle)	UBR2	NP_001179567.1
Danio rerio (zebrafish)	ubr2	XP_005156554.1
Mus musculus (house mouse)	Ubr2	NP_001170845.1
Homo sapiens (human)	UBR2	NP_056070.1
Pan troglodytes (chimpanzee)	UBR2	XP_001134707.1
Canis lupus familiaris (dog)	UBR2	XP_005627416.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ubr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01861	NM_001298427.1 Latest version!	Drosophila melanogaster Ubr1 ubiquitin ligase (Ubr1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa01861	NM_001258794.2 Latest version!	Drosophila melanogaster Ubr1 ubiquitin ligase (Ubr1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa01861	NM_132956.3 Latest version!	Drosophila melanogaster Ubr1 ubiquitin ligase (Ubr1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ubr1 ( NM_001298427.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285356.1 Drosophila melanogaster Ubr1 ubiquitin ligase (Ubr1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01861
Clone ID Related Accession (Same CDS sequence)	NM_001298427.1 , NM_132956.3 , NM_001258794.2
Accession Version	NM_001298427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubr1 ubiquitin ligase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGCT ACGACATGGA GGACGTCGTT GTGGCGCCCC CTGCCGAGTG CAGTTCGCCG 
CTCAAGGAGT GGCGCCTCAA GCGCCAGGCG GGCACACTAG ACCGCTCCGA TATCATCGAG 
TTCTTAAAGC GCGAGTCTCC CAAGTATTTT GATTACCAGA CTAGCGCTAC AGTTAAAGAT 
ACCAATGTGA TTACTCTAAA GTGCATGTTT AAGGAGTCGC TGGCCAAGGA GGAGATCATC 
GATGTCGTTG TGGAGTTTAT GCTGGGCGAC AATCCTTCCT CCGCACTGGA GAAGCTCCGA 
CTGGAGGGCA ACACGGCCAC CGTCTGTGGC AAGGTCTTTA AGAACGGGGA GCCCACCTAC 
AGCTGCCGCG AGTGCGGCGT CGATCCCACC TGCGTGCTTT GCGTCAACTG TTTCAAGCGC 
TCGGCGCATC GCTTTCACAA GTACAAGATG TCCACTTCCG GCGGCGGCGG CTGCTGTGAC 
TGCGGAGACG ACGAGGCCTG GAAGAAGGAT CAGTACTGCG AGCTGCATCT GGCAAATCGC 
AAGAATCCGC TGGAGAGCAA GATCCTGACC GACGCAGTAC TTGAGCGGGT TGAGATCTGC 
TTTGGGGCAA TCCTCGCCTT CTGCGTGAGT TATCTGGAAA TCGAGCCAAA CGCCAGTCTA 
CAGTGCCTGG ATGGAAACGT GGAAGGCGGC CAAGTGGACG GAGCGCAGTA TTGCACTGTG 
CTCTACAACG ACGAGTCGCA CACGTTCGAC CAAGTCATTC AGACGCTGAC CAAAATTGCC 
AAGTGTCGGG CAAAGGACGC CATGGAAATC GTGGCGGCAA TTGATCGCGA GGGACGAGCG 
GTGGTCAAAT GCGATACATT TGAGGAGTGC AACAAACTAA AGGTATCCAT CGAGAACCAA 
ATGATTCTGC CGACCTCCCT GGTGTCGACG GCACGCAACA ATCAGTCACT CAGGACATCG 
GTGCTGCACA TCGGGGCCGT GGCCTGTCAG CAGTTTGCCC TGCAGCTTCT CGGCTGGTTC 
CAGGAGTTCC TGGTGCGACA CTACCTCTTC CGGAAGACGT TCTCCGAGTT GGTGCAGCGC 
AAGCAGGAGA CATTCTGCAT CCGGCACATC CTTGAGTACG ACGTGAAGCT TTGGAAGACG 
GCGCGCACCT GCTGGCACCG CCTCCTGATC TCTGGTATGC TTATGGAGTA CGACAACAAA 
ATGATCCTAG CCCAGGAGTT TTCGCGACGC TACGCCACTA TTGTGGAGGA CTTTATCAGC 
GATGATCATG ATCACGCCTT TTCGATTGTG TCGCTCAGTG TCCAGCTGTT CACGGTGCCC 
AGCATAGCCC ATCATCTGAT CGCTCATGAG GGCATCTTTG ACAAGCTGCT GCACACGTTC 
TACCACGTTG CCATCGAGAA GTTCATTCGT AACAAGACGC TGCACTTTAG CAAGAACATC 
GCCAGCCTCA CCTTCTTCAA GCGGGCTAAC TACATCCTCT ACGATCTGCG ATACCTGCTC 
AGCCTAAAGC CGGATGTGCT GAGCAATGAT TTACGAAACG GTTTCCTAGA AGGCTGCCGG 
GCTTTGATGC GCGTTCTAAA CGTGATGCAG GGCATGGAGT CGATGACCAG ACAGACGGGC 
CAACATATGG ACTACGAGCC AGAGTGGGAG TGCGCCTTCA ACCTGCACAT CAAGCTGGCC 
ACGACCATCT CGCAGGTGAT TGATTGGGCG TCCGGCGATG TGAAGCTGCT GCGTAAGCTC 
TACAAGATGA CAATGCGGGC GCTGGTGAGC AACAGCTTTA TTGTGGGTGG CGAGAAGGTC 
ATGCAGCCGA AAAAAGTGGC GGACCATGTA GCCAACTGTC TAGTTTACGA CATATCAGTG 
CAGCCGGTAT CCATCCACTT GCCTTTGTCG CGTTTCTTCG CCGGAATCTA TCTGCACTTG 
GGTGCCCACG ATTTAACGTA CGACGGGCTG CAGACGGAAA CAGAAGCGTT GAGCATTAAG 
CTAACGCCCA GGGAGATTAT AGAACCGGTT TTGTGCACCC AAGCAATGAT CGCCCAGGTG 
GGCGCAGGAC TGTGGCGCCG CAATGGTTAC ACCCTGCTGC ATCAGCTCTA CTTCTACCGA 
AATGTACGTT GCCGCGTGGA GATGCTGGAC AGGGACATAG CCTGCCTGCA GATAGGCGCC 
TCCCTAATGG AGAGCAACGA GTTCCTGATC CATGTGTTGA ATAGGTTCAA CACGATCCCC 
TGGTTACAGG AAAACTACTG GTCCCTATTG TCGGGTAATG AAATGAACGA TGATATCATT 
CGAGAGGCGT CAATCTTTGA CGAGTTCCTG GAACTTCTGA TCGTGATCAT TGGAGAACGT 
TGGATGCCTG GCGTTTCGAT GGTGACCGAA GAGGATCGCC TGCGCAAGGA GATCATCCAG 
CTGTTGTGTA TCAAGCCATA CTCACACTCG GAGTTGAGTC GCGCCTTGCC AGATGGCAAT 
AGCGGAAATA GCGACAACGT TTTCGAGGAA GTTATCAACA CGGTTGCCGT GTTCAAGAAA 
CCCGTTGGAG CCGACAGCAA GGGGGTGTAC GAACTAAAGG AACATTTGCT TAAGGAGTTC 
AACATGTACT TCTATCACTA CACCAAAGAG GACAAGTCCA AGGCCGAGGA ACTGCAACGG 
GAGCGCCGAA AGGCCAAGAA GCAGCTTGTC TGCTGCCCGC CACCAATGTT GCCAAAACTA 
ACACCAGCCT TCACACCCAT GGCCAACATC CTGCAGTGCC CTGTGTTCCT CAACATCTGC 
AGCTTGATTA TGGAAAGGGC TTTAAATGCT TACAGTCGCA GCTTTACCGA ATCGCATCTT 
CAAAAGGTCC TTCACCTGCT GGGTTATGCC ATTCAGGAGG AGCTTAGCGA GCACTATCCC 
TTCTTGAGCT TCTACGAGCG TTCCCAGGAG TACGGAATTC TGGAGAAGTT GGAAGAGCTC 
GCTCGCTGCC CGAGGTTGGA GGCTCATTAT GACTTTGTGC TGTGGACCAT CGAGCGCTTC 
AAGCAGCTGC AGGCCAAGCA GGCTCCCAGT GATGGTAGAG CAGGACCCAG TTGCTCTCAG 
CAAGGAACCG GAGGGAAATT GTCACTCAGC GCTGAGGAGC AAGCGCGAGA GGAGAGGGAG 
AATCGTGCTC GTCTAGCCGC CGAGCGTCGA GCCCACATTA TGGCCCAGAT GCAGAAAGCC 
CAAAAGTCGT TCATAAGCAG CAATGCAGAA ATGTTTGCGG ACACCGAAAA CGAAACACGC 
AAGGAGTCAG CATCCACGGG TCCAATGGAT TGGGAGGATA TTCCCCCCGA GGAAGAACAG 
GGAGCAGTGG CCCTTGAGTC GAAGGTAGCC TGCTTGGGGC CGGACCGCAA GTTTTATCAC 
GGCACCGATG ATACCTTTAA ATGCATCCTC TGCTTCGAGA ACTGTGCCAT TAGCCGTGGG 
GGCCGGCAAT TGGTTAGCTC TGCCTTTGTC CAAACCTCGC GTGTGATCTT CACTACGCCC 
AATTTGCGTA ATAGTCAGAG CGCACTGCAC ATTAGTTGCT GCGGTCATGT GATGCACTAC 
AGCTGCTGGC TGGAGTACTT CACCAACGAG GAGTTCAAGG AGCTGCGTCG TCCGCACCGC 
AATCGTGCGG CCCTAGCCCA GGCCGCCAAC GTGGAGTTCC AATGTCCGTA TTGCCGAACC 
CTAAGTAACG CTATAATACC GGTAACCGAA ACGCTGCCCG CTTTTTCGGC GCCTCCTTCA 
CCGAACGAAA GCTATTTACC ATTGGACAGT TTCGTGGAGA TCATGAGCAC ACTGGCCATC 
GAACTGGGTA ACGTGAAGGA CCACGAATTG ACAACACTTC CAAGTGTGTC CAACATTTTG 
CGCTTGTCCG GCGTGGTCGG TGGCTTGGCT CAATTCGAGC GGAGCGTGCA GCTCATCAAG 
AATCCTCCTA GACTGCATGC TGACTACATC GAAGGAATTG AATTCCTTAA AAAAGCCCTG 
CTAAATACCA TGAAGATTCA GCAGTCACAT CTAAAGGACC ATCCAGCCAT CGAAAGTATC 
GAAATGGTAC CCATACTGTG GGACAGCTGC AGCTACACGC TGCAGGCACT GGAGATCTAT 
TTGTACGCGG TCGAGAAGCC ATTGAAGGCC GAGCTGTCAA TGCGCCACCA GAGCTGTGCC 
AGGAACTTGG TGAGGGCTTG CTCACGCAGT TCGGCCTTGG AATGGGAAAC TGATCTTCCA 
TTGCTTCCGC CGATGCGTTC CCAGGCGGAG TTTTCCTCTA GACTGCTGGA TACAATCTTC 
AATCAAAATG ACACCAGTGT GCTCGAGTGG GACTGCTTCC GCGTGCTAGT GCCGTTCCAG 
TTTGGAGTGC TCAATCTGAT GGTACCCGAG AAGGGATATA AAACAATCAT ACCCAGCGGC 
AGCATGTTCG ACTTTTACAT CATGCAGACT ATGTTCCTGG CCCAGCTGAC CAAGGCGGTC 
CTCTGCTTTG ACGTGGAGAA GGAGAAGGCC AAACGAGCGG AGAAGGCGCC CAATTCTGAA 
CTTACACAGT TGGATTACAT AGAGCAGCTA CCGTCACGGA TTCGGGACAA CATGATCGAC 
TTTTATCGTC GCTACAATAT CCCAGCGAGA GTGCTGCAGA AGACTAAACA GAAACAGCTG 
GTGGAGGAAG AATCGGAAGA GAATCAGGGT CATGGGCAAA CAGTGGTGAT ACCCTGTGAA 
TCGCATCATT TGGCCCTTCT TCTCGAGTAC GTGCAGCGAC AGATGAGCTC GTTCCTGCGC 
TGCTCCTGTC TCTTCTATCG CTTTCTCACC GATGTGGATT TCCCAGACAC ATTTCCCACA 
GATCAACCGG ATCGCTTTGA TCTGATGTGC CAGTACTTGG GCCTGGACCC CATGCTGGGT 
GTCTACTTTG ACATGGAGAC GGTATACGCC ACCATGATGC ACTCGTTTGC CTCGCATCCT 
CACATTGACC GTGAAGTGGA ACAGCGTTGT CAGCCTGACG CCCGTCGTTC CCTTCAGGTG 
GAACCTTGCC TGCGCCCGCT GCCCCGCTTG AAGGTACTGT GTGATGACTT CAGCGATTTG 
ATCAACAGCG TCTCGGACAT CTTCTGTCCG AACAACGAGC GCGAAGAGAT GAAGACACCA 
ACGATGTGTC TGATATGCGG CTTAATCCTG TGCGGTCAGT CGTACTGCTG CCAGCCAGAG 
CTGGGCAAGG TCTCGGTGGG CGCATGCACT CATCACGCTC ACGCTTGTGG CGCCGAAGTG 
GGCATCTTTC TGCGCATTCG CGACTGCCAG GTGGTGTACC TGGGCCGTGG CAAGGGGTGC 
TTCGTCCCGC CGCCATATTT GGACGAGTAC GGCGAGACGG ATATGGGTCT GAGGCGCGGT 
AATCCGCTGA GACTGTCCCA GGCGGCTTAC AGAAAGATCT ACCTGCAGTG GCTGGGGCAT 
GGGCTGCACG GCGAGATCGC CCGTCTGAAC GACAACGCCA ATGTGGCAGC GGCGGCGCAG 
TGGCATCACA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285356.1
CDS	360..5834
Translation	MDRYDMEDVVVAPPAECSSPLKEWRLKRQAGTLDRSDIIEFLKRESPKYFDYQTSATVKDTNVITLKCMFKESLAKEEIIDVVVEFMLGDNPSSALEKLRLEGNTATVCGKVFKNGEPTYSCRECGVDPTCVLCVNCFKRSAHRFHKYKMSTSGGGGCCDCGDDEAWKKDQYCELHLANRKNPLESKILTDAVLERVEICFGAILAFCVSYLEIEPNASLQCLDGNVEGGQVDGAQYCTVLYNDESHTFDQVIQTLTKIAKCRAKDAMEIVAAIDREGRAVVKCDTFEECNKLKVSIENQMILPTSLVSTARNNQSLRTSVLHIGAVACQQFALQLLGWFQEFLVRHYLFRKTFSELVQRKQETFCIRHILEYDVKLWKTARTCWHRLLISGMLMEYDNKMILAQEFSRRYATIVEDFISDDHDHAFSIVSLSVQLFTVPSIAHHLIAHEGIFDKLLHTFYHVAIEKFIRNKTLHFSKNIASLTFFKRANYILYDLRYLLSLKPDVLSNDLRNGFLEGCRALMRVLNVMQGMESMTRQTGQHMDYEPEWECAFNLHIKLATTISQVIDWASGDVKLLRKLYKMTMRALVSNSFIVGGEKVMQPKKVADHVANCLVYDISVQPVSIHLPLSRFFAGIYLHLGAHDLTYDGLQTETEALSIKLTPREIIEPVLCTQAMIAQVGAGLWRRNGYTLLHQLYFYRNVRCRVEMLDRDIACLQIGASLMESNEFLIHVLNRFNTIPWLQENYWSLLSGNEMNDDIIREASIFDEFLELLIVIIGERWMPGVSMVTEEDRLRKEIIQLLCIKPYSHSELSRALPDGNSGNSDNVFEEVINTVAVFKKPVGADSKGVYELKEHLLKEFNMYFYHYTKEDKSKAEELQRERRKAKKQLVCCPPPMLPKLTPAFTPMANILQCPVFLNICSLIMERALNAYSRSFTESHLQKVLHLLGYAIQEELSEHYPFLSFYERSQEYGILEKLEELARCPRLEAHYDFVLWTIERFKQLQAKQAPSDGRAGPSCSQQGTGGKLSLSAEEQAREERENRARLAAERRAHIMAQMQKAQKSFISSNAEMFADTENETRKESASTGPMDWEDIPPEEEQGAVALESKVACLGPDRKFYHGTDDTFKCILCFENCAISRGGRQLVSSAFVQTSRVIFTTPNLRNSQSALHISCCGHVMHYSCWLEYFTNEEFKELRRPHRNRAALAQAANVEFQCPYCRTLSNAIIPVTETLPAFSAPPSPNESYLPLDSFVEIMSTLAIELGNVKDHELTTLPSVSNILRLSGVVGGLAQFERSVQLIKNPPRLHADYIEGIEFLKKALLNTMKIQQSHLKDHPAIESIEMVPILWDSCSYTLQALEIYLYAVEKPLKAELSMRHQSCARNLVRACSRSSALEWETDLPLLPPMRSQAEFSSRLLDTIFNQNDTSVLEWDCFRVLVPFQFGVLNLMVPEKGYKTIIPSGSMFDFYIMQTMFLAQLTKAVLCFDVEKEKAKRAEKAPNSELTQLDYIEQLPSRIRDNMIDFYRRYNIPARVLQKTKQKQLVEEESEENQGHGQTVVIPCESHHLALLLEYVQRQMSSFLRCSCLFYRFLTDVDFPDTFPTDQPDRFDLMCQYLGLDPMLGVYFDMETVYATMMHSFASHPHIDREVEQRCQPDARRSLQVEPCLRPLPRLKVLCDDFSDLINSVSDIFCPNNEREEMKTPTMCLICGLILCGQSYCCQPELGKVSVGACTHHAHACGAEVGIFLRIRDCQVVYLGRGKGCFVPPPYLDEYGETDMGLRRGNPLRLSQAAYRKIYLQWLGHGLHGEIARLNDNANVAAAAQWHHM

Target ORF information:

RefSeq Version	NM_001298427.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubr1 ubiquitin ligase (Ubr1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298427.1

ORF Insert Sequence:

ATGGATCGCT ACGACATGGA GGACGTCGTT GTGGCGCCCC CTGCCGAGTG CAGTTCGCCG 
CTCAAGGAGT GGCGCCTCAA GCGCCAGGCG GGCACACTAG ACCGCTCCGA TATCATCGAG 
TTCTTAAAGC GCGAGTCTCC CAAGTATTTT GATTACCAGA CTAGCGCTAC AGTTAAAGAT 
ACCAATGTGA TTACTCTAAA GTGCATGTTT AAGGAGTCGC TGGCCAAGGA GGAGATCATC 
GATGTCGTTG TGGAGTTTAT GCTGGGCGAC AATCCTTCCT CCGCACTGGA GAAGCTCCGA 
CTGGAGGGCA ACACGGCCAC CGTCTGTGGC AAGGTCTTTA AGAACGGGGA GCCCACCTAC 
AGCTGCCGCG AGTGCGGCGT CGATCCCACC TGCGTGCTTT GCGTCAACTG TTTCAAGCGC 
TCGGCGCATC GCTTTCACAA GTACAAGATG TCCACTTCCG GCGGCGGCGG CTGCTGTGAC 
TGCGGAGACG ACGAGGCCTG GAAGAAGGAT CAGTACTGCG AGCTGCATCT GGCAAATCGC 
AAGAATCCGC TGGAGAGCAA GATCCTGACC GACGCAGTAC TTGAGCGGGT TGAGATCTGC 
TTTGGGGCAA TCCTCGCCTT CTGCGTGAGT TATCTGGAAA TCGAGCCAAA CGCCAGTCTA 
CAGTGCCTGG ATGGAAACGT GGAAGGCGGC CAAGTGGACG GAGCGCAGTA TTGCACTGTG 
CTCTACAACG ACGAGTCGCA CACGTTCGAC CAAGTCATTC AGACGCTGAC CAAAATTGCC 
AAGTGTCGGG CAAAGGACGC CATGGAAATC GTGGCGGCAA TTGATCGCGA GGGACGAGCG 
GTGGTCAAAT GCGATACATT TGAGGAGTGC AACAAACTAA AGGTATCCAT CGAGAACCAA 
ATGATTCTGC CGACCTCCCT GGTGTCGACG GCACGCAACA ATCAGTCACT CAGGACATCG 
GTGCTGCACA TCGGGGCCGT GGCCTGTCAG CAGTTTGCCC TGCAGCTTCT CGGCTGGTTC 
CAGGAGTTCC TGGTGCGACA CTACCTCTTC CGGAAGACGT TCTCCGAGTT GGTGCAGCGC 
AAGCAGGAGA CATTCTGCAT CCGGCACATC CTTGAGTACG ACGTGAAGCT TTGGAAGACG 
GCGCGCACCT GCTGGCACCG CCTCCTGATC TCTGGTATGC TTATGGAGTA CGACAACAAA 
ATGATCCTAG CCCAGGAGTT TTCGCGACGC TACGCCACTA TTGTGGAGGA CTTTATCAGC 
GATGATCATG ATCACGCCTT TTCGATTGTG TCGCTCAGTG TCCAGCTGTT CACGGTGCCC 
AGCATAGCCC ATCATCTGAT CGCTCATGAG GGCATCTTTG ACAAGCTGCT GCACACGTTC 
TACCACGTTG CCATCGAGAA GTTCATTCGT AACAAGACGC TGCACTTTAG CAAGAACATC 
GCCAGCCTCA CCTTCTTCAA GCGGGCTAAC TACATCCTCT ACGATCTGCG ATACCTGCTC 
AGCCTAAAGC CGGATGTGCT GAGCAATGAT TTACGAAACG GTTTCCTAGA AGGCTGCCGG 
GCTTTGATGC GCGTTCTAAA CGTGATGCAG GGCATGGAGT CGATGACCAG ACAGACGGGC 
CAACATATGG ACTACGAGCC AGAGTGGGAG TGCGCCTTCA ACCTGCACAT CAAGCTGGCC 
ACGACCATCT CGCAGGTGAT TGATTGGGCG TCCGGCGATG TGAAGCTGCT GCGTAAGCTC 
TACAAGATGA CAATGCGGGC GCTGGTGAGC AACAGCTTTA TTGTGGGTGG CGAGAAGGTC 
ATGCAGCCGA AAAAAGTGGC GGACCATGTA GCCAACTGTC TAGTTTACGA CATATCAGTG 
CAGCCGGTAT CCATCCACTT GCCTTTGTCG CGTTTCTTCG CCGGAATCTA TCTGCACTTG 
GGTGCCCACG ATTTAACGTA CGACGGGCTG CAGACGGAAA CAGAAGCGTT GAGCATTAAG 
CTAACGCCCA GGGAGATTAT AGAACCGGTT TTGTGCACCC AAGCAATGAT CGCCCAGGTG 
GGCGCAGGAC TGTGGCGCCG CAATGGTTAC ACCCTGCTGC ATCAGCTCTA CTTCTACCGA 
AATGTACGTT GCCGCGTGGA GATGCTGGAC AGGGACATAG CCTGCCTGCA GATAGGCGCC 
TCCCTAATGG AGAGCAACGA GTTCCTGATC CATGTGTTGA ATAGGTTCAA CACGATCCCC 
TGGTTACAGG AAAACTACTG GTCCCTATTG TCGGGTAATG AAATGAACGA TGATATCATT 
CGAGAGGCGT CAATCTTTGA CGAGTTCCTG GAACTTCTGA TCGTGATCAT TGGAGAACGT 
TGGATGCCTG GCGTTTCGAT GGTGACCGAA GAGGATCGCC TGCGCAAGGA GATCATCCAG 
CTGTTGTGTA TCAAGCCATA CTCACACTCG GAGTTGAGTC GCGCCTTGCC AGATGGCAAT 
AGCGGAAATA GCGACAACGT TTTCGAGGAA GTTATCAACA CGGTTGCCGT GTTCAAGAAA 
CCCGTTGGAG CCGACAGCAA GGGGGTGTAC GAACTAAAGG AACATTTGCT TAAGGAGTTC 
AACATGTACT TCTATCACTA CACCAAAGAG GACAAGTCCA AGGCCGAGGA ACTGCAACGG 
GAGCGCCGAA AGGCCAAGAA GCAGCTTGTC TGCTGCCCGC CACCAATGTT GCCAAAACTA 
ACACCAGCCT TCACACCCAT GGCCAACATC CTGCAGTGCC CTGTGTTCCT CAACATCTGC 
AGCTTGATTA TGGAAAGGGC TTTAAATGCT TACAGTCGCA GCTTTACCGA ATCGCATCTT 
CAAAAGGTCC TTCACCTGCT GGGTTATGCC ATTCAGGAGG AGCTTAGCGA GCACTATCCC 
TTCTTGAGCT TCTACGAGCG TTCCCAGGAG TACGGAATTC TGGAGAAGTT GGAAGAGCTC 
GCTCGCTGCC CGAGGTTGGA GGCTCATTAT GACTTTGTGC TGTGGACCAT CGAGCGCTTC 
AAGCAGCTGC AGGCCAAGCA GGCTCCCAGT GATGGTAGAG CAGGACCCAG TTGCTCTCAG 
CAAGGAACCG GAGGGAAATT GTCACTCAGC GCTGAGGAGC AAGCGCGAGA GGAGAGGGAG 
AATCGTGCTC GTCTAGCCGC CGAGCGTCGA GCCCACATTA TGGCCCAGAT GCAGAAAGCC 
CAAAAGTCGT TCATAAGCAG CAATGCAGAA ATGTTTGCGG ACACCGAAAA CGAAACACGC 
AAGGAGTCAG CATCCACGGG TCCAATGGAT TGGGAGGATA TTCCCCCCGA GGAAGAACAG 
GGAGCAGTGG CCCTTGAGTC GAAGGTAGCC TGCTTGGGGC CGGACCGCAA GTTTTATCAC 
GGCACCGATG ATACCTTTAA ATGCATCCTC TGCTTCGAGA ACTGTGCCAT TAGCCGTGGG 
GGCCGGCAAT TGGTTAGCTC TGCCTTTGTC CAAACCTCGC GTGTGATCTT CACTACGCCC 
AATTTGCGTA ATAGTCAGAG CGCACTGCAC ATTAGTTGCT GCGGTCATGT GATGCACTAC 
AGCTGCTGGC TGGAGTACTT CACCAACGAG GAGTTCAAGG AGCTGCGTCG TCCGCACCGC 
AATCGTGCGG CCCTAGCCCA GGCCGCCAAC GTGGAGTTCC AATGTCCGTA TTGCCGAACC 
CTAAGTAACG CTATAATACC GGTAACCGAA ACGCTGCCCG CTTTTTCGGC GCCTCCTTCA 
CCGAACGAAA GCTATTTACC ATTGGACAGT TTCGTGGAGA TCATGAGCAC ACTGGCCATC 
GAACTGGGTA ACGTGAAGGA CCACGAATTG ACAACACTTC CAAGTGTGTC CAACATTTTG 
CGCTTGTCCG GCGTGGTCGG TGGCTTGGCT CAATTCGAGC GGAGCGTGCA GCTCATCAAG 
AATCCTCCTA GACTGCATGC TGACTACATC GAAGGAATTG AATTCCTTAA AAAAGCCCTG 
CTAAATACCA TGAAGATTCA GCAGTCACAT CTAAAGGACC ATCCAGCCAT CGAAAGTATC 
GAAATGGTAC CCATACTGTG GGACAGCTGC AGCTACACGC TGCAGGCACT GGAGATCTAT 
TTGTACGCGG TCGAGAAGCC ATTGAAGGCC GAGCTGTCAA TGCGCCACCA GAGCTGTGCC 
AGGAACTTGG TGAGGGCTTG CTCACGCAGT TCGGCCTTGG AATGGGAAAC TGATCTTCCA 
TTGCTTCCGC CGATGCGTTC CCAGGCGGAG TTTTCCTCTA GACTGCTGGA TACAATCTTC 
AATCAAAATG ACACCAGTGT GCTCGAGTGG GACTGCTTCC GCGTGCTAGT GCCGTTCCAG 
TTTGGAGTGC TCAATCTGAT GGTACCCGAG AAGGGATATA AAACAATCAT ACCCAGCGGC 
AGCATGTTCG ACTTTTACAT CATGCAGACT ATGTTCCTGG CCCAGCTGAC CAAGGCGGTC 
CTCTGCTTTG ACGTGGAGAA GGAGAAGGCC AAACGAGCGG AGAAGGCGCC CAATTCTGAA 
CTTACACAGT TGGATTACAT AGAGCAGCTA CCGTCACGGA TTCGGGACAA CATGATCGAC 
TTTTATCGTC GCTACAATAT CCCAGCGAGA GTGCTGCAGA AGACTAAACA GAAACAGCTG 
GTGGAGGAAG AATCGGAAGA GAATCAGGGT CATGGGCAAA CAGTGGTGAT ACCCTGTGAA 
TCGCATCATT TGGCCCTTCT TCTCGAGTAC GTGCAGCGAC AGATGAGCTC GTTCCTGCGC 
TGCTCCTGTC TCTTCTATCG CTTTCTCACC GATGTGGATT TCCCAGACAC ATTTCCCACA 
GATCAACCGG ATCGCTTTGA TCTGATGTGC CAGTACTTGG GCCTGGACCC CATGCTGGGT 
GTCTACTTTG ACATGGAGAC GGTATACGCC ACCATGATGC ACTCGTTTGC CTCGCATCCT 
CACATTGACC GTGAAGTGGA ACAGCGTTGT CAGCCTGACG CCCGTCGTTC CCTTCAGGTG 
GAACCTTGCC TGCGCCCGCT GCCCCGCTTG AAGGTACTGT GTGATGACTT CAGCGATTTG 
ATCAACAGCG TCTCGGACAT CTTCTGTCCG AACAACGAGC GCGAAGAGAT GAAGACACCA 
ACGATGTGTC TGATATGCGG CTTAATCCTG TGCGGTCAGT CGTACTGCTG CCAGCCAGAG 
CTGGGCAAGG TCTCGGTGGG CGCATGCACT CATCACGCTC ACGCTTGTGG CGCCGAAGTG 
GGCATCTTTC TGCGCATTCG CGACTGCCAG GTGGTGTACC TGGGCCGTGG CAAGGGGTGC 
TTCGTCCCGC CGCCATATTT GGACGAGTAC GGCGAGACGG ATATGGGTCT GAGGCGCGGT 
AATCCGCTGA GACTGTCCCA GGCGGCTTAC AGAAAGATCT ACCTGCAGTG GCTGGGGCAT 
GGGCTGCACG GCGAGATCGC CCGTCTGAAC GACAACGCCA ATGTGGCAGC GGCGGCGCAG 
TGGCATCACA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr1 ( NM_132956.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573184.1 Drosophila melanogaster Ubr1 ubiquitin ligase (Ubr1), transcript variant A, mRNA.

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CloneID	OFa01861
Clone ID Related Accession (Same CDS sequence)	NM_001298427.1 , NM_132956.3 , NM_001258794.2
Accession Version	NM_132956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubr1 ubiquitin ligase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132956.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGCT ACGACATGGA GGACGTCGTT GTGGCGCCCC CTGCCGAGTG CAGTTCGCCG 
CTCAAGGAGT GGCGCCTCAA GCGCCAGGCG GGCACACTAG ACCGCTCCGA TATCATCGAG 
TTCTTAAAGC GCGAGTCTCC CAAGTATTTT GATTACCAGA CTAGCGCTAC AGTTAAAGAT 
ACCAATGTGA TTACTCTAAA GTGCATGTTT AAGGAGTCGC TGGCCAAGGA GGAGATCATC 
GATGTCGTTG TGGAGTTTAT GCTGGGCGAC AATCCTTCCT CCGCACTGGA GAAGCTCCGA 
CTGGAGGGCA ACACGGCCAC CGTCTGTGGC AAGGTCTTTA AGAACGGGGA GCCCACCTAC 
AGCTGCCGCG AGTGCGGCGT CGATCCCACC TGCGTGCTTT GCGTCAACTG TTTCAAGCGC 
TCGGCGCATC GCTTTCACAA GTACAAGATG TCCACTTCCG GCGGCGGCGG CTGCTGTGAC 
TGCGGAGACG ACGAGGCCTG GAAGAAGGAT CAGTACTGCG AGCTGCATCT GGCAAATCGC 
AAGAATCCGC TGGAGAGCAA GATCCTGACC GACGCAGTAC TTGAGCGGGT TGAGATCTGC 
TTTGGGGCAA TCCTCGCCTT CTGCGTGAGT TATCTGGAAA TCGAGCCAAA CGCCAGTCTA 
CAGTGCCTGG ATGGAAACGT GGAAGGCGGC CAAGTGGACG GAGCGCAGTA TTGCACTGTG 
CTCTACAACG ACGAGTCGCA CACGTTCGAC CAAGTCATTC AGACGCTGAC CAAAATTGCC 
AAGTGTCGGG CAAAGGACGC CATGGAAATC GTGGCGGCAA TTGATCGCGA GGGACGAGCG 
GTGGTCAAAT GCGATACATT TGAGGAGTGC AACAAACTAA AGGTATCCAT CGAGAACCAA 
ATGATTCTGC CGACCTCCCT GGTGTCGACG GCACGCAACA ATCAGTCACT CAGGACATCG 
GTGCTGCACA TCGGGGCCGT GGCCTGTCAG CAGTTTGCCC TGCAGCTTCT CGGCTGGTTC 
CAGGAGTTCC TGGTGCGACA CTACCTCTTC CGGAAGACGT TCTCCGAGTT GGTGCAGCGC 
AAGCAGGAGA CATTCTGCAT CCGGCACATC CTTGAGTACG ACGTGAAGCT TTGGAAGACG 
GCGCGCACCT GCTGGCACCG CCTCCTGATC TCTGGTATGC TTATGGAGTA CGACAACAAA 
ATGATCCTAG CCCAGGAGTT TTCGCGACGC TACGCCACTA TTGTGGAGGA CTTTATCAGC 
GATGATCATG ATCACGCCTT TTCGATTGTG TCGCTCAGTG TCCAGCTGTT CACGGTGCCC 
AGCATAGCCC ATCATCTGAT CGCTCATGAG GGCATCTTTG ACAAGCTGCT GCACACGTTC 
TACCACGTTG CCATCGAGAA GTTCATTCGT AACAAGACGC TGCACTTTAG CAAGAACATC 
GCCAGCCTCA CCTTCTTCAA GCGGGCTAAC TACATCCTCT ACGATCTGCG ATACCTGCTC 
AGCCTAAAGC CGGATGTGCT GAGCAATGAT TTACGAAACG GTTTCCTAGA AGGCTGCCGG 
GCTTTGATGC GCGTTCTAAA CGTGATGCAG GGCATGGAGT CGATGACCAG ACAGACGGGC 
CAACATATGG ACTACGAGCC AGAGTGGGAG TGCGCCTTCA ACCTGCACAT CAAGCTGGCC 
ACGACCATCT CGCAGGTGAT TGATTGGGCG TCCGGCGATG TGAAGCTGCT GCGTAAGCTC 
TACAAGATGA CAATGCGGGC GCTGGTGAGC AACAGCTTTA TTGTGGGTGG CGAGAAGGTC 
ATGCAGCCGA AAAAAGTGGC GGACCATGTA GCCAACTGTC TAGTTTACGA CATATCAGTG 
CAGCCGGTAT CCATCCACTT GCCTTTGTCG CGTTTCTTCG CCGGAATCTA TCTGCACTTG 
GGTGCCCACG ATTTAACGTA CGACGGGCTG CAGACGGAAA CAGAAGCGTT GAGCATTAAG 
CTAACGCCCA GGGAGATTAT AGAACCGGTT TTGTGCACCC AAGCAATGAT CGCCCAGGTG 
GGCGCAGGAC TGTGGCGCCG CAATGGTTAC ACCCTGCTGC ATCAGCTCTA CTTCTACCGA 
AATGTACGTT GCCGCGTGGA GATGCTGGAC AGGGACATAG CCTGCCTGCA GATAGGCGCC 
TCCCTAATGG AGAGCAACGA GTTCCTGATC CATGTGTTGA ATAGGTTCAA CACGATCCCC 
TGGTTACAGG AAAACTACTG GTCCCTATTG TCGGGTAATG AAATGAACGA TGATATCATT 
CGAGAGGCGT CAATCTTTGA CGAGTTCCTG GAACTTCTGA TCGTGATCAT TGGAGAACGT 
TGGATGCCTG GCGTTTCGAT GGTGACCGAA GAGGATCGCC TGCGCAAGGA GATCATCCAG 
CTGTTGTGTA TCAAGCCATA CTCACACTCG GAGTTGAGTC GCGCCTTGCC AGATGGCAAT 
AGCGGAAATA GCGACAACGT TTTCGAGGAA GTTATCAACA CGGTTGCCGT GTTCAAGAAA 
CCCGTTGGAG CCGACAGCAA GGGGGTGTAC GAACTAAAGG AACATTTGCT TAAGGAGTTC 
AACATGTACT TCTATCACTA CACCAAAGAG GACAAGTCCA AGGCCGAGGA ACTGCAACGG 
GAGCGCCGAA AGGCCAAGAA GCAGCTTGTC TGCTGCCCGC CACCAATGTT GCCAAAACTA 
ACACCAGCCT TCACACCCAT GGCCAACATC CTGCAGTGCC CTGTGTTCCT CAACATCTGC 
AGCTTGATTA TGGAAAGGGC TTTAAATGCT TACAGTCGCA GCTTTACCGA ATCGCATCTT 
CAAAAGGTCC TTCACCTGCT GGGTTATGCC ATTCAGGAGG AGCTTAGCGA GCACTATCCC 
TTCTTGAGCT TCTACGAGCG TTCCCAGGAG TACGGAATTC TGGAGAAGTT GGAAGAGCTC 
GCTCGCTGCC CGAGGTTGGA GGCTCATTAT GACTTTGTGC TGTGGACCAT CGAGCGCTTC 
AAGCAGCTGC AGGCCAAGCA GGCTCCCAGT GATGGTAGAG CAGGACCCAG TTGCTCTCAG 
CAAGGAACCG GAGGGAAATT GTCACTCAGC GCTGAGGAGC AAGCGCGAGA GGAGAGGGAG 
AATCGTGCTC GTCTAGCCGC CGAGCGTCGA GCCCACATTA TGGCCCAGAT GCAGAAAGCC 
CAAAAGTCGT TCATAAGCAG CAATGCAGAA ATGTTTGCGG ACACCGAAAA CGAAACACGC 
AAGGAGTCAG CATCCACGGG TCCAATGGAT TGGGAGGATA TTCCCCCCGA GGAAGAACAG 
GGAGCAGTGG CCCTTGAGTC GAAGGTAGCC TGCTTGGGGC CGGACCGCAA GTTTTATCAC 
GGCACCGATG ATACCTTTAA ATGCATCCTC TGCTTCGAGA ACTGTGCCAT TAGCCGTGGG 
GGCCGGCAAT TGGTTAGCTC TGCCTTTGTC CAAACCTCGC GTGTGATCTT CACTACGCCC 
AATTTGCGTA ATAGTCAGAG CGCACTGCAC ATTAGTTGCT GCGGTCATGT GATGCACTAC 
AGCTGCTGGC TGGAGTACTT CACCAACGAG GAGTTCAAGG AGCTGCGTCG TCCGCACCGC 
AATCGTGCGG CCCTAGCCCA GGCCGCCAAC GTGGAGTTCC AATGTCCGTA TTGCCGAACC 
CTAAGTAACG CTATAATACC GGTAACCGAA ACGCTGCCCG CTTTTTCGGC GCCTCCTTCA 
CCGAACGAAA GCTATTTACC ATTGGACAGT TTCGTGGAGA TCATGAGCAC ACTGGCCATC 
GAACTGGGTA ACGTGAAGGA CCACGAATTG ACAACACTTC CAAGTGTGTC CAACATTTTG 
CGCTTGTCCG GCGTGGTCGG TGGCTTGGCT CAATTCGAGC GGAGCGTGCA GCTCATCAAG 
AATCCTCCTA GACTGCATGC TGACTACATC GAAGGAATTG AATTCCTTAA AAAAGCCCTG 
CTAAATACCA TGAAGATTCA GCAGTCACAT CTAAAGGACC ATCCAGCCAT CGAAAGTATC 
GAAATGGTAC CCATACTGTG GGACAGCTGC AGCTACACGC TGCAGGCACT GGAGATCTAT 
TTGTACGCGG TCGAGAAGCC ATTGAAGGCC GAGCTGTCAA TGCGCCACCA GAGCTGTGCC 
AGGAACTTGG TGAGGGCTTG CTCACGCAGT TCGGCCTTGG AATGGGAAAC TGATCTTCCA 
TTGCTTCCGC CGATGCGTTC CCAGGCGGAG TTTTCCTCTA GACTGCTGGA TACAATCTTC 
AATCAAAATG ACACCAGTGT GCTCGAGTGG GACTGCTTCC GCGTGCTAGT GCCGTTCCAG 
TTTGGAGTGC TCAATCTGAT GGTACCCGAG AAGGGATATA AAACAATCAT ACCCAGCGGC 
AGCATGTTCG ACTTTTACAT CATGCAGACT ATGTTCCTGG CCCAGCTGAC CAAGGCGGTC 
CTCTGCTTTG ACGTGGAGAA GGAGAAGGCC AAACGAGCGG AGAAGGCGCC CAATTCTGAA 
CTTACACAGT TGGATTACAT AGAGCAGCTA CCGTCACGGA TTCGGGACAA CATGATCGAC 
TTTTATCGTC GCTACAATAT CCCAGCGAGA GTGCTGCAGA AGACTAAACA GAAACAGCTG 
GTGGAGGAAG AATCGGAAGA GAATCAGGGT CATGGGCAAA CAGTGGTGAT ACCCTGTGAA 
TCGCATCATT TGGCCCTTCT TCTCGAGTAC GTGCAGCGAC AGATGAGCTC GTTCCTGCGC 
TGCTCCTGTC TCTTCTATCG CTTTCTCACC GATGTGGATT TCCCAGACAC ATTTCCCACA 
GATCAACCGG ATCGCTTTGA TCTGATGTGC CAGTACTTGG GCCTGGACCC CATGCTGGGT 
GTCTACTTTG ACATGGAGAC GGTATACGCC ACCATGATGC ACTCGTTTGC CTCGCATCCT 
CACATTGACC GTGAAGTGGA ACAGCGTTGT CAGCCTGACG CCCGTCGTTC CCTTCAGGTG 
GAACCTTGCC TGCGCCCGCT GCCCCGCTTG AAGGTACTGT GTGATGACTT CAGCGATTTG 
ATCAACAGCG TCTCGGACAT CTTCTGTCCG AACAACGAGC GCGAAGAGAT GAAGACACCA 
ACGATGTGTC TGATATGCGG CTTAATCCTG TGCGGTCAGT CGTACTGCTG CCAGCCAGAG 
CTGGGCAAGG TCTCGGTGGG CGCATGCACT CATCACGCTC ACGCTTGTGG CGCCGAAGTG 
GGCATCTTTC TGCGCATTCG CGACTGCCAG GTGGTGTACC TGGGCCGTGG CAAGGGGTGC 
TTCGTCCCGC CGCCATATTT GGACGAGTAC GGCGAGACGG ATATGGGTCT GAGGCGCGGT 
AATCCGCTGA GACTGTCCCA GGCGGCTTAC AGAAAGATCT ACCTGCAGTG GCTGGGGCAT 
GGGCTGCACG GCGAGATCGC CCGTCTGAAC GACAACGCCA ATGTGGCAGC GGCGGCGCAG 
TGGCATCACA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573184.1
CDS	360..5834
Translation	MDRYDMEDVVVAPPAECSSPLKEWRLKRQAGTLDRSDIIEFLKRESPKYFDYQTSATVKDTNVITLKCMFKESLAKEEIIDVVVEFMLGDNPSSALEKLRLEGNTATVCGKVFKNGEPTYSCRECGVDPTCVLCVNCFKRSAHRFHKYKMSTSGGGGCCDCGDDEAWKKDQYCELHLANRKNPLESKILTDAVLERVEICFGAILAFCVSYLEIEPNASLQCLDGNVEGGQVDGAQYCTVLYNDESHTFDQVIQTLTKIAKCRAKDAMEIVAAIDREGRAVVKCDTFEECNKLKVSIENQMILPTSLVSTARNNQSLRTSVLHIGAVACQQFALQLLGWFQEFLVRHYLFRKTFSELVQRKQETFCIRHILEYDVKLWKTARTCWHRLLISGMLMEYDNKMILAQEFSRRYATIVEDFISDDHDHAFSIVSLSVQLFTVPSIAHHLIAHEGIFDKLLHTFYHVAIEKFIRNKTLHFSKNIASLTFFKRANYILYDLRYLLSLKPDVLSNDLRNGFLEGCRALMRVLNVMQGMESMTRQTGQHMDYEPEWECAFNLHIKLATTISQVIDWASGDVKLLRKLYKMTMRALVSNSFIVGGEKVMQPKKVADHVANCLVYDISVQPVSIHLPLSRFFAGIYLHLGAHDLTYDGLQTETEALSIKLTPREIIEPVLCTQAMIAQVGAGLWRRNGYTLLHQLYFYRNVRCRVEMLDRDIACLQIGASLMESNEFLIHVLNRFNTIPWLQENYWSLLSGNEMNDDIIREASIFDEFLELLIVIIGERWMPGVSMVTEEDRLRKEIIQLLCIKPYSHSELSRALPDGNSGNSDNVFEEVINTVAVFKKPVGADSKGVYELKEHLLKEFNMYFYHYTKEDKSKAEELQRERRKAKKQLVCCPPPMLPKLTPAFTPMANILQCPVFLNICSLIMERALNAYSRSFTESHLQKVLHLLGYAIQEELSEHYPFLSFYERSQEYGILEKLEELARCPRLEAHYDFVLWTIERFKQLQAKQAPSDGRAGPSCSQQGTGGKLSLSAEEQAREERENRARLAAERRAHIMAQMQKAQKSFISSNAEMFADTENETRKESASTGPMDWEDIPPEEEQGAVALESKVACLGPDRKFYHGTDDTFKCILCFENCAISRGGRQLVSSAFVQTSRVIFTTPNLRNSQSALHISCCGHVMHYSCWLEYFTNEEFKELRRPHRNRAALAQAANVEFQCPYCRTLSNAIIPVTETLPAFSAPPSPNESYLPLDSFVEIMSTLAIELGNVKDHELTTLPSVSNILRLSGVVGGLAQFERSVQLIKNPPRLHADYIEGIEFLKKALLNTMKIQQSHLKDHPAIESIEMVPILWDSCSYTLQALEIYLYAVEKPLKAELSMRHQSCARNLVRACSRSSALEWETDLPLLPPMRSQAEFSSRLLDTIFNQNDTSVLEWDCFRVLVPFQFGVLNLMVPEKGYKTIIPSGSMFDFYIMQTMFLAQLTKAVLCFDVEKEKAKRAEKAPNSELTQLDYIEQLPSRIRDNMIDFYRRYNIPARVLQKTKQKQLVEEESEENQGHGQTVVIPCESHHLALLLEYVQRQMSSFLRCSCLFYRFLTDVDFPDTFPTDQPDRFDLMCQYLGLDPMLGVYFDMETVYATMMHSFASHPHIDREVEQRCQPDARRSLQVEPCLRPLPRLKVLCDDFSDLINSVSDIFCPNNEREEMKTPTMCLICGLILCGQSYCCQPELGKVSVGACTHHAHACGAEVGIFLRIRDCQVVYLGRGKGCFVPPPYLDEYGETDMGLRRGNPLRLSQAAYRKIYLQWLGHGLHGEIARLNDNANVAAAAQWHHM

Target ORF information:

RefSeq Version	NM_132956.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubr1 ubiquitin ligase (Ubr1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132956.3

ORF Insert Sequence:

ATGGATCGCT ACGACATGGA GGACGTCGTT GTGGCGCCCC CTGCCGAGTG CAGTTCGCCG 
CTCAAGGAGT GGCGCCTCAA GCGCCAGGCG GGCACACTAG ACCGCTCCGA TATCATCGAG 
TTCTTAAAGC GCGAGTCTCC CAAGTATTTT GATTACCAGA CTAGCGCTAC AGTTAAAGAT 
ACCAATGTGA TTACTCTAAA GTGCATGTTT AAGGAGTCGC TGGCCAAGGA GGAGATCATC 
GATGTCGTTG TGGAGTTTAT GCTGGGCGAC AATCCTTCCT CCGCACTGGA GAAGCTCCGA 
CTGGAGGGCA ACACGGCCAC CGTCTGTGGC AAGGTCTTTA AGAACGGGGA GCCCACCTAC 
AGCTGCCGCG AGTGCGGCGT CGATCCCACC TGCGTGCTTT GCGTCAACTG TTTCAAGCGC 
TCGGCGCATC GCTTTCACAA GTACAAGATG TCCACTTCCG GCGGCGGCGG CTGCTGTGAC 
TGCGGAGACG ACGAGGCCTG GAAGAAGGAT CAGTACTGCG AGCTGCATCT GGCAAATCGC 
AAGAATCCGC TGGAGAGCAA GATCCTGACC GACGCAGTAC TTGAGCGGGT TGAGATCTGC 
TTTGGGGCAA TCCTCGCCTT CTGCGTGAGT TATCTGGAAA TCGAGCCAAA CGCCAGTCTA 
CAGTGCCTGG ATGGAAACGT GGAAGGCGGC CAAGTGGACG GAGCGCAGTA TTGCACTGTG 
CTCTACAACG ACGAGTCGCA CACGTTCGAC CAAGTCATTC AGACGCTGAC CAAAATTGCC 
AAGTGTCGGG CAAAGGACGC CATGGAAATC GTGGCGGCAA TTGATCGCGA GGGACGAGCG 
GTGGTCAAAT GCGATACATT TGAGGAGTGC AACAAACTAA AGGTATCCAT CGAGAACCAA 
ATGATTCTGC CGACCTCCCT GGTGTCGACG GCACGCAACA ATCAGTCACT CAGGACATCG 
GTGCTGCACA TCGGGGCCGT GGCCTGTCAG CAGTTTGCCC TGCAGCTTCT CGGCTGGTTC 
CAGGAGTTCC TGGTGCGACA CTACCTCTTC CGGAAGACGT TCTCCGAGTT GGTGCAGCGC 
AAGCAGGAGA CATTCTGCAT CCGGCACATC CTTGAGTACG ACGTGAAGCT TTGGAAGACG 
GCGCGCACCT GCTGGCACCG CCTCCTGATC TCTGGTATGC TTATGGAGTA CGACAACAAA 
ATGATCCTAG CCCAGGAGTT TTCGCGACGC TACGCCACTA TTGTGGAGGA CTTTATCAGC 
GATGATCATG ATCACGCCTT TTCGATTGTG TCGCTCAGTG TCCAGCTGTT CACGGTGCCC 
AGCATAGCCC ATCATCTGAT CGCTCATGAG GGCATCTTTG ACAAGCTGCT GCACACGTTC 
TACCACGTTG CCATCGAGAA GTTCATTCGT AACAAGACGC TGCACTTTAG CAAGAACATC 
GCCAGCCTCA CCTTCTTCAA GCGGGCTAAC TACATCCTCT ACGATCTGCG ATACCTGCTC 
AGCCTAAAGC CGGATGTGCT GAGCAATGAT TTACGAAACG GTTTCCTAGA AGGCTGCCGG 
GCTTTGATGC GCGTTCTAAA CGTGATGCAG GGCATGGAGT CGATGACCAG ACAGACGGGC 
CAACATATGG ACTACGAGCC AGAGTGGGAG TGCGCCTTCA ACCTGCACAT CAAGCTGGCC 
ACGACCATCT CGCAGGTGAT TGATTGGGCG TCCGGCGATG TGAAGCTGCT GCGTAAGCTC 
TACAAGATGA CAATGCGGGC GCTGGTGAGC AACAGCTTTA TTGTGGGTGG CGAGAAGGTC 
ATGCAGCCGA AAAAAGTGGC GGACCATGTA GCCAACTGTC TAGTTTACGA CATATCAGTG 
CAGCCGGTAT CCATCCACTT GCCTTTGTCG CGTTTCTTCG CCGGAATCTA TCTGCACTTG 
GGTGCCCACG ATTTAACGTA CGACGGGCTG CAGACGGAAA CAGAAGCGTT GAGCATTAAG 
CTAACGCCCA GGGAGATTAT AGAACCGGTT TTGTGCACCC AAGCAATGAT CGCCCAGGTG 
GGCGCAGGAC TGTGGCGCCG CAATGGTTAC ACCCTGCTGC ATCAGCTCTA CTTCTACCGA 
AATGTACGTT GCCGCGTGGA GATGCTGGAC AGGGACATAG CCTGCCTGCA GATAGGCGCC 
TCCCTAATGG AGAGCAACGA GTTCCTGATC CATGTGTTGA ATAGGTTCAA CACGATCCCC 
TGGTTACAGG AAAACTACTG GTCCCTATTG TCGGGTAATG AAATGAACGA TGATATCATT 
CGAGAGGCGT CAATCTTTGA CGAGTTCCTG GAACTTCTGA TCGTGATCAT TGGAGAACGT 
TGGATGCCTG GCGTTTCGAT GGTGACCGAA GAGGATCGCC TGCGCAAGGA GATCATCCAG 
CTGTTGTGTA TCAAGCCATA CTCACACTCG GAGTTGAGTC GCGCCTTGCC AGATGGCAAT 
AGCGGAAATA GCGACAACGT TTTCGAGGAA GTTATCAACA CGGTTGCCGT GTTCAAGAAA 
CCCGTTGGAG CCGACAGCAA GGGGGTGTAC GAACTAAAGG AACATTTGCT TAAGGAGTTC 
AACATGTACT TCTATCACTA CACCAAAGAG GACAAGTCCA AGGCCGAGGA ACTGCAACGG 
GAGCGCCGAA AGGCCAAGAA GCAGCTTGTC TGCTGCCCGC CACCAATGTT GCCAAAACTA 
ACACCAGCCT TCACACCCAT GGCCAACATC CTGCAGTGCC CTGTGTTCCT CAACATCTGC 
AGCTTGATTA TGGAAAGGGC TTTAAATGCT TACAGTCGCA GCTTTACCGA ATCGCATCTT 
CAAAAGGTCC TTCACCTGCT GGGTTATGCC ATTCAGGAGG AGCTTAGCGA GCACTATCCC 
TTCTTGAGCT TCTACGAGCG TTCCCAGGAG TACGGAATTC TGGAGAAGTT GGAAGAGCTC 
GCTCGCTGCC CGAGGTTGGA GGCTCATTAT GACTTTGTGC TGTGGACCAT CGAGCGCTTC 
AAGCAGCTGC AGGCCAAGCA GGCTCCCAGT GATGGTAGAG CAGGACCCAG TTGCTCTCAG 
CAAGGAACCG GAGGGAAATT GTCACTCAGC GCTGAGGAGC AAGCGCGAGA GGAGAGGGAG 
AATCGTGCTC GTCTAGCCGC CGAGCGTCGA GCCCACATTA TGGCCCAGAT GCAGAAAGCC 
CAAAAGTCGT TCATAAGCAG CAATGCAGAA ATGTTTGCGG ACACCGAAAA CGAAACACGC 
AAGGAGTCAG CATCCACGGG TCCAATGGAT TGGGAGGATA TTCCCCCCGA GGAAGAACAG 
GGAGCAGTGG CCCTTGAGTC GAAGGTAGCC TGCTTGGGGC CGGACCGCAA GTTTTATCAC 
GGCACCGATG ATACCTTTAA ATGCATCCTC TGCTTCGAGA ACTGTGCCAT TAGCCGTGGG 
GGCCGGCAAT TGGTTAGCTC TGCCTTTGTC CAAACCTCGC GTGTGATCTT CACTACGCCC 
AATTTGCGTA ATAGTCAGAG CGCACTGCAC ATTAGTTGCT GCGGTCATGT GATGCACTAC 
AGCTGCTGGC TGGAGTACTT CACCAACGAG GAGTTCAAGG AGCTGCGTCG TCCGCACCGC 
AATCGTGCGG CCCTAGCCCA GGCCGCCAAC GTGGAGTTCC AATGTCCGTA TTGCCGAACC 
CTAAGTAACG CTATAATACC GGTAACCGAA ACGCTGCCCG CTTTTTCGGC GCCTCCTTCA 
CCGAACGAAA GCTATTTACC ATTGGACAGT TTCGTGGAGA TCATGAGCAC ACTGGCCATC 
GAACTGGGTA ACGTGAAGGA CCACGAATTG ACAACACTTC CAAGTGTGTC CAACATTTTG 
CGCTTGTCCG GCGTGGTCGG TGGCTTGGCT CAATTCGAGC GGAGCGTGCA GCTCATCAAG 
AATCCTCCTA GACTGCATGC TGACTACATC GAAGGAATTG AATTCCTTAA AAAAGCCCTG 
CTAAATACCA TGAAGATTCA GCAGTCACAT CTAAAGGACC ATCCAGCCAT CGAAAGTATC 
GAAATGGTAC CCATACTGTG GGACAGCTGC AGCTACACGC TGCAGGCACT GGAGATCTAT 
TTGTACGCGG TCGAGAAGCC ATTGAAGGCC GAGCTGTCAA TGCGCCACCA GAGCTGTGCC 
AGGAACTTGG TGAGGGCTTG CTCACGCAGT TCGGCCTTGG AATGGGAAAC TGATCTTCCA 
TTGCTTCCGC CGATGCGTTC CCAGGCGGAG TTTTCCTCTA GACTGCTGGA TACAATCTTC 
AATCAAAATG ACACCAGTGT GCTCGAGTGG GACTGCTTCC GCGTGCTAGT GCCGTTCCAG 
TTTGGAGTGC TCAATCTGAT GGTACCCGAG AAGGGATATA AAACAATCAT ACCCAGCGGC 
AGCATGTTCG ACTTTTACAT CATGCAGACT ATGTTCCTGG CCCAGCTGAC CAAGGCGGTC 
CTCTGCTTTG ACGTGGAGAA GGAGAAGGCC AAACGAGCGG AGAAGGCGCC CAATTCTGAA 
CTTACACAGT TGGATTACAT AGAGCAGCTA CCGTCACGGA TTCGGGACAA CATGATCGAC 
TTTTATCGTC GCTACAATAT CCCAGCGAGA GTGCTGCAGA AGACTAAACA GAAACAGCTG 
GTGGAGGAAG AATCGGAAGA GAATCAGGGT CATGGGCAAA CAGTGGTGAT ACCCTGTGAA 
TCGCATCATT TGGCCCTTCT TCTCGAGTAC GTGCAGCGAC AGATGAGCTC GTTCCTGCGC 
TGCTCCTGTC TCTTCTATCG CTTTCTCACC GATGTGGATT TCCCAGACAC ATTTCCCACA 
GATCAACCGG ATCGCTTTGA TCTGATGTGC CAGTACTTGG GCCTGGACCC CATGCTGGGT 
GTCTACTTTG ACATGGAGAC GGTATACGCC ACCATGATGC ACTCGTTTGC CTCGCATCCT 
CACATTGACC GTGAAGTGGA ACAGCGTTGT CAGCCTGACG CCCGTCGTTC CCTTCAGGTG 
GAACCTTGCC TGCGCCCGCT GCCCCGCTTG AAGGTACTGT GTGATGACTT CAGCGATTTG 
ATCAACAGCG TCTCGGACAT CTTCTGTCCG AACAACGAGC GCGAAGAGAT GAAGACACCA 
ACGATGTGTC TGATATGCGG CTTAATCCTG TGCGGTCAGT CGTACTGCTG CCAGCCAGAG 
CTGGGCAAGG TCTCGGTGGG CGCATGCACT CATCACGCTC ACGCTTGTGG CGCCGAAGTG 
GGCATCTTTC TGCGCATTCG CGACTGCCAG GTGGTGTACC TGGGCCGTGG CAAGGGGTGC 
TTCGTCCCGC CGCCATATTT GGACGAGTAC GGCGAGACGG ATATGGGTCT GAGGCGCGGT 
AATCCGCTGA GACTGTCCCA GGCGGCTTAC AGAAAGATCT ACCTGCAGTG GCTGGGGCAT 
GGGCTGCACG GCGAGATCGC CCGTCTGAAC GACAACGCCA ATGTGGCAGC GGCGGCGCAG 
TGGCATCACA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr1 ( NM_001258794.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245723.1 Drosophila melanogaster Ubr1 ubiquitin ligase (Ubr1), transcript variant B, mRNA.

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CloneID	OFa01861
Clone ID Related Accession (Same CDS sequence)	NM_001298427.1 , NM_132956.3 , NM_001258794.2
Accession Version	NM_001258794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubr1 ubiquitin ligase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258794.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGCT ACGACATGGA GGACGTCGTT GTGGCGCCCC CTGCCGAGTG CAGTTCGCCG 
CTCAAGGAGT GGCGCCTCAA GCGCCAGGCG GGCACACTAG ACCGCTCCGA TATCATCGAG 
TTCTTAAAGC GCGAGTCTCC CAAGTATTTT GATTACCAGA CTAGCGCTAC AGTTAAAGAT 
ACCAATGTGA TTACTCTAAA GTGCATGTTT AAGGAGTCGC TGGCCAAGGA GGAGATCATC 
GATGTCGTTG TGGAGTTTAT GCTGGGCGAC AATCCTTCCT CCGCACTGGA GAAGCTCCGA 
CTGGAGGGCA ACACGGCCAC CGTCTGTGGC AAGGTCTTTA AGAACGGGGA GCCCACCTAC 
AGCTGCCGCG AGTGCGGCGT CGATCCCACC TGCGTGCTTT GCGTCAACTG TTTCAAGCGC 
TCGGCGCATC GCTTTCACAA GTACAAGATG TCCACTTCCG GCGGCGGCGG CTGCTGTGAC 
TGCGGAGACG ACGAGGCCTG GAAGAAGGAT CAGTACTGCG AGCTGCATCT GGCAAATCGC 
AAGAATCCGC TGGAGAGCAA GATCCTGACC GACGCAGTAC TTGAGCGGGT TGAGATCTGC 
TTTGGGGCAA TCCTCGCCTT CTGCGTGAGT TATCTGGAAA TCGAGCCAAA CGCCAGTCTA 
CAGTGCCTGG ATGGAAACGT GGAAGGCGGC CAAGTGGACG GAGCGCAGTA TTGCACTGTG 
CTCTACAACG ACGAGTCGCA CACGTTCGAC CAAGTCATTC AGACGCTGAC CAAAATTGCC 
AAGTGTCGGG CAAAGGACGC CATGGAAATC GTGGCGGCAA TTGATCGCGA GGGACGAGCG 
GTGGTCAAAT GCGATACATT TGAGGAGTGC AACAAACTAA AGGTATCCAT CGAGAACCAA 
ATGATTCTGC CGACCTCCCT GGTGTCGACG GCACGCAACA ATCAGTCACT CAGGACATCG 
GTGCTGCACA TCGGGGCCGT GGCCTGTCAG CAGTTTGCCC TGCAGCTTCT CGGCTGGTTC 
CAGGAGTTCC TGGTGCGACA CTACCTCTTC CGGAAGACGT TCTCCGAGTT GGTGCAGCGC 
AAGCAGGAGA CATTCTGCAT CCGGCACATC CTTGAGTACG ACGTGAAGCT TTGGAAGACG 
GCGCGCACCT GCTGGCACCG CCTCCTGATC TCTGGTATGC TTATGGAGTA CGACAACAAA 
ATGATCCTAG CCCAGGAGTT TTCGCGACGC TACGCCACTA TTGTGGAGGA CTTTATCAGC 
GATGATCATG ATCACGCCTT TTCGATTGTG TCGCTCAGTG TCCAGCTGTT CACGGTGCCC 
AGCATAGCCC ATCATCTGAT CGCTCATGAG GGCATCTTTG ACAAGCTGCT GCACACGTTC 
TACCACGTTG CCATCGAGAA GTTCATTCGT AACAAGACGC TGCACTTTAG CAAGAACATC 
GCCAGCCTCA CCTTCTTCAA GCGGGCTAAC TACATCCTCT ACGATCTGCG ATACCTGCTC 
AGCCTAAAGC CGGATGTGCT GAGCAATGAT TTACGAAACG GTTTCCTAGA AGGCTGCCGG 
GCTTTGATGC GCGTTCTAAA CGTGATGCAG GGCATGGAGT CGATGACCAG ACAGACGGGC 
CAACATATGG ACTACGAGCC AGAGTGGGAG TGCGCCTTCA ACCTGCACAT CAAGCTGGCC 
ACGACCATCT CGCAGGTGAT TGATTGGGCG TCCGGCGATG TGAAGCTGCT GCGTAAGCTC 
TACAAGATGA CAATGCGGGC GCTGGTGAGC AACAGCTTTA TTGTGGGTGG CGAGAAGGTC 
ATGCAGCCGA AAAAAGTGGC GGACCATGTA GCCAACTGTC TAGTTTACGA CATATCAGTG 
CAGCCGGTAT CCATCCACTT GCCTTTGTCG CGTTTCTTCG CCGGAATCTA TCTGCACTTG 
GGTGCCCACG ATTTAACGTA CGACGGGCTG CAGACGGAAA CAGAAGCGTT GAGCATTAAG 
CTAACGCCCA GGGAGATTAT AGAACCGGTT TTGTGCACCC AAGCAATGAT CGCCCAGGTG 
GGCGCAGGAC TGTGGCGCCG CAATGGTTAC ACCCTGCTGC ATCAGCTCTA CTTCTACCGA 
AATGTACGTT GCCGCGTGGA GATGCTGGAC AGGGACATAG CCTGCCTGCA GATAGGCGCC 
TCCCTAATGG AGAGCAACGA GTTCCTGATC CATGTGTTGA ATAGGTTCAA CACGATCCCC 
TGGTTACAGG AAAACTACTG GTCCCTATTG TCGGGTAATG AAATGAACGA TGATATCATT 
CGAGAGGCGT CAATCTTTGA CGAGTTCCTG GAACTTCTGA TCGTGATCAT TGGAGAACGT 
TGGATGCCTG GCGTTTCGAT GGTGACCGAA GAGGATCGCC TGCGCAAGGA GATCATCCAG 
CTGTTGTGTA TCAAGCCATA CTCACACTCG GAGTTGAGTC GCGCCTTGCC AGATGGCAAT 
AGCGGAAATA GCGACAACGT TTTCGAGGAA GTTATCAACA CGGTTGCCGT GTTCAAGAAA 
CCCGTTGGAG CCGACAGCAA GGGGGTGTAC GAACTAAAGG AACATTTGCT TAAGGAGTTC 
AACATGTACT TCTATCACTA CACCAAAGAG GACAAGTCCA AGGCCGAGGA ACTGCAACGG 
GAGCGCCGAA AGGCCAAGAA GCAGCTTGTC TGCTGCCCGC CACCAATGTT GCCAAAACTA 
ACACCAGCCT TCACACCCAT GGCCAACATC CTGCAGTGCC CTGTGTTCCT CAACATCTGC 
AGCTTGATTA TGGAAAGGGC TTTAAATGCT TACAGTCGCA GCTTTACCGA ATCGCATCTT 
CAAAAGGTCC TTCACCTGCT GGGTTATGCC ATTCAGGAGG AGCTTAGCGA GCACTATCCC 
TTCTTGAGCT TCTACGAGCG TTCCCAGGAG TACGGAATTC TGGAGAAGTT GGAAGAGCTC 
GCTCGCTGCC CGAGGTTGGA GGCTCATTAT GACTTTGTGC TGTGGACCAT CGAGCGCTTC 
AAGCAGCTGC AGGCCAAGCA GGCTCCCAGT GATGGTAGAG CAGGACCCAG TTGCTCTCAG 
CAAGGAACCG GAGGGAAATT GTCACTCAGC GCTGAGGAGC AAGCGCGAGA GGAGAGGGAG 
AATCGTGCTC GTCTAGCCGC CGAGCGTCGA GCCCACATTA TGGCCCAGAT GCAGAAAGCC 
CAAAAGTCGT TCATAAGCAG CAATGCAGAA ATGTTTGCGG ACACCGAAAA CGAAACACGC 
AAGGAGTCAG CATCCACGGG TCCAATGGAT TGGGAGGATA TTCCCCCCGA GGAAGAACAG 
GGAGCAGTGG CCCTTGAGTC GAAGGTAGCC TGCTTGGGGC CGGACCGCAA GTTTTATCAC 
GGCACCGATG ATACCTTTAA ATGCATCCTC TGCTTCGAGA ACTGTGCCAT TAGCCGTGGG 
GGCCGGCAAT TGGTTAGCTC TGCCTTTGTC CAAACCTCGC GTGTGATCTT CACTACGCCC 
AATTTGCGTA ATAGTCAGAG CGCACTGCAC ATTAGTTGCT GCGGTCATGT GATGCACTAC 
AGCTGCTGGC TGGAGTACTT CACCAACGAG GAGTTCAAGG AGCTGCGTCG TCCGCACCGC 
AATCGTGCGG CCCTAGCCCA GGCCGCCAAC GTGGAGTTCC AATGTCCGTA TTGCCGAACC 
CTAAGTAACG CTATAATACC GGTAACCGAA ACGCTGCCCG CTTTTTCGGC GCCTCCTTCA 
CCGAACGAAA GCTATTTACC ATTGGACAGT TTCGTGGAGA TCATGAGCAC ACTGGCCATC 
GAACTGGGTA ACGTGAAGGA CCACGAATTG ACAACACTTC CAAGTGTGTC CAACATTTTG 
CGCTTGTCCG GCGTGGTCGG TGGCTTGGCT CAATTCGAGC GGAGCGTGCA GCTCATCAAG 
AATCCTCCTA GACTGCATGC TGACTACATC GAAGGAATTG AATTCCTTAA AAAAGCCCTG 
CTAAATACCA TGAAGATTCA GCAGTCACAT CTAAAGGACC ATCCAGCCAT CGAAAGTATC 
GAAATGGTAC CCATACTGTG GGACAGCTGC AGCTACACGC TGCAGGCACT GGAGATCTAT 
TTGTACGCGG TCGAGAAGCC ATTGAAGGCC GAGCTGTCAA TGCGCCACCA GAGCTGTGCC 
AGGAACTTGG TGAGGGCTTG CTCACGCAGT TCGGCCTTGG AATGGGAAAC TGATCTTCCA 
TTGCTTCCGC CGATGCGTTC CCAGGCGGAG TTTTCCTCTA GACTGCTGGA TACAATCTTC 
AATCAAAATG ACACCAGTGT GCTCGAGTGG GACTGCTTCC GCGTGCTAGT GCCGTTCCAG 
TTTGGAGTGC TCAATCTGAT GGTACCCGAG AAGGGATATA AAACAATCAT ACCCAGCGGC 
AGCATGTTCG ACTTTTACAT CATGCAGACT ATGTTCCTGG CCCAGCTGAC CAAGGCGGTC 
CTCTGCTTTG ACGTGGAGAA GGAGAAGGCC AAACGAGCGG AGAAGGCGCC CAATTCTGAA 
CTTACACAGT TGGATTACAT AGAGCAGCTA CCGTCACGGA TTCGGGACAA CATGATCGAC 
TTTTATCGTC GCTACAATAT CCCAGCGAGA GTGCTGCAGA AGACTAAACA GAAACAGCTG 
GTGGAGGAAG AATCGGAAGA GAATCAGGGT CATGGGCAAA CAGTGGTGAT ACCCTGTGAA 
TCGCATCATT TGGCCCTTCT TCTCGAGTAC GTGCAGCGAC AGATGAGCTC GTTCCTGCGC 
TGCTCCTGTC TCTTCTATCG CTTTCTCACC GATGTGGATT TCCCAGACAC ATTTCCCACA 
GATCAACCGG ATCGCTTTGA TCTGATGTGC CAGTACTTGG GCCTGGACCC CATGCTGGGT 
GTCTACTTTG ACATGGAGAC GGTATACGCC ACCATGATGC ACTCGTTTGC CTCGCATCCT 
CACATTGACC GTGAAGTGGA ACAGCGTTGT CAGCCTGACG CCCGTCGTTC CCTTCAGGTG 
GAACCTTGCC TGCGCCCGCT GCCCCGCTTG AAGGTACTGT GTGATGACTT CAGCGATTTG 
ATCAACAGCG TCTCGGACAT CTTCTGTCCG AACAACGAGC GCGAAGAGAT GAAGACACCA 
ACGATGTGTC TGATATGCGG CTTAATCCTG TGCGGTCAGT CGTACTGCTG CCAGCCAGAG 
CTGGGCAAGG TCTCGGTGGG CGCATGCACT CATCACGCTC ACGCTTGTGG CGCCGAAGTG 
GGCATCTTTC TGCGCATTCG CGACTGCCAG GTGGTGTACC TGGGCCGTGG CAAGGGGTGC 
TTCGTCCCGC CGCCATATTT GGACGAGTAC GGCGAGACGG ATATGGGTCT GAGGCGCGGT 
AATCCGCTGA GACTGTCCCA GGCGGCTTAC AGAAAGATCT ACCTGCAGTG GCTGGGGCAT 
GGGCTGCACG GCGAGATCGC CCGTCTGAAC GACAACGCCA ATGTGGCAGC GGCGGCGCAG 
TGGCATCACA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245723.1
CDS	218..5692
Translation	MDRYDMEDVVVAPPAECSSPLKEWRLKRQAGTLDRSDIIEFLKRESPKYFDYQTSATVKDTNVITLKCMFKESLAKEEIIDVVVEFMLGDNPSSALEKLRLEGNTATVCGKVFKNGEPTYSCRECGVDPTCVLCVNCFKRSAHRFHKYKMSTSGGGGCCDCGDDEAWKKDQYCELHLANRKNPLESKILTDAVLERVEICFGAILAFCVSYLEIEPNASLQCLDGNVEGGQVDGAQYCTVLYNDESHTFDQVIQTLTKIAKCRAKDAMEIVAAIDREGRAVVKCDTFEECNKLKVSIENQMILPTSLVSTARNNQSLRTSVLHIGAVACQQFALQLLGWFQEFLVRHYLFRKTFSELVQRKQETFCIRHILEYDVKLWKTARTCWHRLLISGMLMEYDNKMILAQEFSRRYATIVEDFISDDHDHAFSIVSLSVQLFTVPSIAHHLIAHEGIFDKLLHTFYHVAIEKFIRNKTLHFSKNIASLTFFKRANYILYDLRYLLSLKPDVLSNDLRNGFLEGCRALMRVLNVMQGMESMTRQTGQHMDYEPEWECAFNLHIKLATTISQVIDWASGDVKLLRKLYKMTMRALVSNSFIVGGEKVMQPKKVADHVANCLVYDISVQPVSIHLPLSRFFAGIYLHLGAHDLTYDGLQTETEALSIKLTPREIIEPVLCTQAMIAQVGAGLWRRNGYTLLHQLYFYRNVRCRVEMLDRDIACLQIGASLMESNEFLIHVLNRFNTIPWLQENYWSLLSGNEMNDDIIREASIFDEFLELLIVIIGERWMPGVSMVTEEDRLRKEIIQLLCIKPYSHSELSRALPDGNSGNSDNVFEEVINTVAVFKKPVGADSKGVYELKEHLLKEFNMYFYHYTKEDKSKAEELQRERRKAKKQLVCCPPPMLPKLTPAFTPMANILQCPVFLNICSLIMERALNAYSRSFTESHLQKVLHLLGYAIQEELSEHYPFLSFYERSQEYGILEKLEELARCPRLEAHYDFVLWTIERFKQLQAKQAPSDGRAGPSCSQQGTGGKLSLSAEEQAREERENRARLAAERRAHIMAQMQKAQKSFISSNAEMFADTENETRKESASTGPMDWEDIPPEEEQGAVALESKVACLGPDRKFYHGTDDTFKCILCFENCAISRGGRQLVSSAFVQTSRVIFTTPNLRNSQSALHISCCGHVMHYSCWLEYFTNEEFKELRRPHRNRAALAQAANVEFQCPYCRTLSNAIIPVTETLPAFSAPPSPNESYLPLDSFVEIMSTLAIELGNVKDHELTTLPSVSNILRLSGVVGGLAQFERSVQLIKNPPRLHADYIEGIEFLKKALLNTMKIQQSHLKDHPAIESIEMVPILWDSCSYTLQALEIYLYAVEKPLKAELSMRHQSCARNLVRACSRSSALEWETDLPLLPPMRSQAEFSSRLLDTIFNQNDTSVLEWDCFRVLVPFQFGVLNLMVPEKGYKTIIPSGSMFDFYIMQTMFLAQLTKAVLCFDVEKEKAKRAEKAPNSELTQLDYIEQLPSRIRDNMIDFYRRYNIPARVLQKTKQKQLVEEESEENQGHGQTVVIPCESHHLALLLEYVQRQMSSFLRCSCLFYRFLTDVDFPDTFPTDQPDRFDLMCQYLGLDPMLGVYFDMETVYATMMHSFASHPHIDREVEQRCQPDARRSLQVEPCLRPLPRLKVLCDDFSDLINSVSDIFCPNNEREEMKTPTMCLICGLILCGQSYCCQPELGKVSVGACTHHAHACGAEVGIFLRIRDCQVVYLGRGKGCFVPPPYLDEYGETDMGLRRGNPLRLSQAAYRKIYLQWLGHGLHGEIARLNDNANVAAAAQWHHM

Target ORF information:

RefSeq Version	NM_001258794.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubr1 ubiquitin ligase (Ubr1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258794.2

ORF Insert Sequence:

ATGGATCGCT ACGACATGGA GGACGTCGTT GTGGCGCCCC CTGCCGAGTG CAGTTCGCCG 
CTCAAGGAGT GGCGCCTCAA GCGCCAGGCG GGCACACTAG ACCGCTCCGA TATCATCGAG 
TTCTTAAAGC GCGAGTCTCC CAAGTATTTT GATTACCAGA CTAGCGCTAC AGTTAAAGAT 
ACCAATGTGA TTACTCTAAA GTGCATGTTT AAGGAGTCGC TGGCCAAGGA GGAGATCATC 
GATGTCGTTG TGGAGTTTAT GCTGGGCGAC AATCCTTCCT CCGCACTGGA GAAGCTCCGA 
CTGGAGGGCA ACACGGCCAC CGTCTGTGGC AAGGTCTTTA AGAACGGGGA GCCCACCTAC 
AGCTGCCGCG AGTGCGGCGT CGATCCCACC TGCGTGCTTT GCGTCAACTG TTTCAAGCGC 
TCGGCGCATC GCTTTCACAA GTACAAGATG TCCACTTCCG GCGGCGGCGG CTGCTGTGAC 
TGCGGAGACG ACGAGGCCTG GAAGAAGGAT CAGTACTGCG AGCTGCATCT GGCAAATCGC 
AAGAATCCGC TGGAGAGCAA GATCCTGACC GACGCAGTAC TTGAGCGGGT TGAGATCTGC 
TTTGGGGCAA TCCTCGCCTT CTGCGTGAGT TATCTGGAAA TCGAGCCAAA CGCCAGTCTA 
CAGTGCCTGG ATGGAAACGT GGAAGGCGGC CAAGTGGACG GAGCGCAGTA TTGCACTGTG 
CTCTACAACG ACGAGTCGCA CACGTTCGAC CAAGTCATTC AGACGCTGAC CAAAATTGCC 
AAGTGTCGGG CAAAGGACGC CATGGAAATC GTGGCGGCAA TTGATCGCGA GGGACGAGCG 
GTGGTCAAAT GCGATACATT TGAGGAGTGC AACAAACTAA AGGTATCCAT CGAGAACCAA 
ATGATTCTGC CGACCTCCCT GGTGTCGACG GCACGCAACA ATCAGTCACT CAGGACATCG 
GTGCTGCACA TCGGGGCCGT GGCCTGTCAG CAGTTTGCCC TGCAGCTTCT CGGCTGGTTC 
CAGGAGTTCC TGGTGCGACA CTACCTCTTC CGGAAGACGT TCTCCGAGTT GGTGCAGCGC 
AAGCAGGAGA CATTCTGCAT CCGGCACATC CTTGAGTACG ACGTGAAGCT TTGGAAGACG 
GCGCGCACCT GCTGGCACCG CCTCCTGATC TCTGGTATGC TTATGGAGTA CGACAACAAA 
ATGATCCTAG CCCAGGAGTT TTCGCGACGC TACGCCACTA TTGTGGAGGA CTTTATCAGC 
GATGATCATG ATCACGCCTT TTCGATTGTG TCGCTCAGTG TCCAGCTGTT CACGGTGCCC 
AGCATAGCCC ATCATCTGAT CGCTCATGAG GGCATCTTTG ACAAGCTGCT GCACACGTTC 
TACCACGTTG CCATCGAGAA GTTCATTCGT AACAAGACGC TGCACTTTAG CAAGAACATC 
GCCAGCCTCA CCTTCTTCAA GCGGGCTAAC TACATCCTCT ACGATCTGCG ATACCTGCTC 
AGCCTAAAGC CGGATGTGCT GAGCAATGAT TTACGAAACG GTTTCCTAGA AGGCTGCCGG 
GCTTTGATGC GCGTTCTAAA CGTGATGCAG GGCATGGAGT CGATGACCAG ACAGACGGGC 
CAACATATGG ACTACGAGCC AGAGTGGGAG TGCGCCTTCA ACCTGCACAT CAAGCTGGCC 
ACGACCATCT CGCAGGTGAT TGATTGGGCG TCCGGCGATG TGAAGCTGCT GCGTAAGCTC 
TACAAGATGA CAATGCGGGC GCTGGTGAGC AACAGCTTTA TTGTGGGTGG CGAGAAGGTC 
ATGCAGCCGA AAAAAGTGGC GGACCATGTA GCCAACTGTC TAGTTTACGA CATATCAGTG 
CAGCCGGTAT CCATCCACTT GCCTTTGTCG CGTTTCTTCG CCGGAATCTA TCTGCACTTG 
GGTGCCCACG ATTTAACGTA CGACGGGCTG CAGACGGAAA CAGAAGCGTT GAGCATTAAG 
CTAACGCCCA GGGAGATTAT AGAACCGGTT TTGTGCACCC AAGCAATGAT CGCCCAGGTG 
GGCGCAGGAC TGTGGCGCCG CAATGGTTAC ACCCTGCTGC ATCAGCTCTA CTTCTACCGA 
AATGTACGTT GCCGCGTGGA GATGCTGGAC AGGGACATAG CCTGCCTGCA GATAGGCGCC 
TCCCTAATGG AGAGCAACGA GTTCCTGATC CATGTGTTGA ATAGGTTCAA CACGATCCCC 
TGGTTACAGG AAAACTACTG GTCCCTATTG TCGGGTAATG AAATGAACGA TGATATCATT 
CGAGAGGCGT CAATCTTTGA CGAGTTCCTG GAACTTCTGA TCGTGATCAT TGGAGAACGT 
TGGATGCCTG GCGTTTCGAT GGTGACCGAA GAGGATCGCC TGCGCAAGGA GATCATCCAG 
CTGTTGTGTA TCAAGCCATA CTCACACTCG GAGTTGAGTC GCGCCTTGCC AGATGGCAAT 
AGCGGAAATA GCGACAACGT TTTCGAGGAA GTTATCAACA CGGTTGCCGT GTTCAAGAAA 
CCCGTTGGAG CCGACAGCAA GGGGGTGTAC GAACTAAAGG AACATTTGCT TAAGGAGTTC 
AACATGTACT TCTATCACTA CACCAAAGAG GACAAGTCCA AGGCCGAGGA ACTGCAACGG 
GAGCGCCGAA AGGCCAAGAA GCAGCTTGTC TGCTGCCCGC CACCAATGTT GCCAAAACTA 
ACACCAGCCT TCACACCCAT GGCCAACATC CTGCAGTGCC CTGTGTTCCT CAACATCTGC 
AGCTTGATTA TGGAAAGGGC TTTAAATGCT TACAGTCGCA GCTTTACCGA ATCGCATCTT 
CAAAAGGTCC TTCACCTGCT GGGTTATGCC ATTCAGGAGG AGCTTAGCGA GCACTATCCC 
TTCTTGAGCT TCTACGAGCG TTCCCAGGAG TACGGAATTC TGGAGAAGTT GGAAGAGCTC 
GCTCGCTGCC CGAGGTTGGA GGCTCATTAT GACTTTGTGC TGTGGACCAT CGAGCGCTTC 
AAGCAGCTGC AGGCCAAGCA GGCTCCCAGT GATGGTAGAG CAGGACCCAG TTGCTCTCAG 
CAAGGAACCG GAGGGAAATT GTCACTCAGC GCTGAGGAGC AAGCGCGAGA GGAGAGGGAG 
AATCGTGCTC GTCTAGCCGC CGAGCGTCGA GCCCACATTA TGGCCCAGAT GCAGAAAGCC 
CAAAAGTCGT TCATAAGCAG CAATGCAGAA ATGTTTGCGG ACACCGAAAA CGAAACACGC 
AAGGAGTCAG CATCCACGGG TCCAATGGAT TGGGAGGATA TTCCCCCCGA GGAAGAACAG 
GGAGCAGTGG CCCTTGAGTC GAAGGTAGCC TGCTTGGGGC CGGACCGCAA GTTTTATCAC 
GGCACCGATG ATACCTTTAA ATGCATCCTC TGCTTCGAGA ACTGTGCCAT TAGCCGTGGG 
GGCCGGCAAT TGGTTAGCTC TGCCTTTGTC CAAACCTCGC GTGTGATCTT CACTACGCCC 
AATTTGCGTA ATAGTCAGAG CGCACTGCAC ATTAGTTGCT GCGGTCATGT GATGCACTAC 
AGCTGCTGGC TGGAGTACTT CACCAACGAG GAGTTCAAGG AGCTGCGTCG TCCGCACCGC 
AATCGTGCGG CCCTAGCCCA GGCCGCCAAC GTGGAGTTCC AATGTCCGTA TTGCCGAACC 
CTAAGTAACG CTATAATACC GGTAACCGAA ACGCTGCCCG CTTTTTCGGC GCCTCCTTCA 
CCGAACGAAA GCTATTTACC ATTGGACAGT TTCGTGGAGA TCATGAGCAC ACTGGCCATC 
GAACTGGGTA ACGTGAAGGA CCACGAATTG ACAACACTTC CAAGTGTGTC CAACATTTTG 
CGCTTGTCCG GCGTGGTCGG TGGCTTGGCT CAATTCGAGC GGAGCGTGCA GCTCATCAAG 
AATCCTCCTA GACTGCATGC TGACTACATC GAAGGAATTG AATTCCTTAA AAAAGCCCTG 
CTAAATACCA TGAAGATTCA GCAGTCACAT CTAAAGGACC ATCCAGCCAT CGAAAGTATC 
GAAATGGTAC CCATACTGTG GGACAGCTGC AGCTACACGC TGCAGGCACT GGAGATCTAT 
TTGTACGCGG TCGAGAAGCC ATTGAAGGCC GAGCTGTCAA TGCGCCACCA GAGCTGTGCC 
AGGAACTTGG TGAGGGCTTG CTCACGCAGT TCGGCCTTGG AATGGGAAAC TGATCTTCCA 
TTGCTTCCGC CGATGCGTTC CCAGGCGGAG TTTTCCTCTA GACTGCTGGA TACAATCTTC 
AATCAAAATG ACACCAGTGT GCTCGAGTGG GACTGCTTCC GCGTGCTAGT GCCGTTCCAG 
TTTGGAGTGC TCAATCTGAT GGTACCCGAG AAGGGATATA AAACAATCAT ACCCAGCGGC 
AGCATGTTCG ACTTTTACAT CATGCAGACT ATGTTCCTGG CCCAGCTGAC CAAGGCGGTC 
CTCTGCTTTG ACGTGGAGAA GGAGAAGGCC AAACGAGCGG AGAAGGCGCC CAATTCTGAA 
CTTACACAGT TGGATTACAT AGAGCAGCTA CCGTCACGGA TTCGGGACAA CATGATCGAC 
TTTTATCGTC GCTACAATAT CCCAGCGAGA GTGCTGCAGA AGACTAAACA GAAACAGCTG 
GTGGAGGAAG AATCGGAAGA GAATCAGGGT CATGGGCAAA CAGTGGTGAT ACCCTGTGAA 
TCGCATCATT TGGCCCTTCT TCTCGAGTAC GTGCAGCGAC AGATGAGCTC GTTCCTGCGC 
TGCTCCTGTC TCTTCTATCG CTTTCTCACC GATGTGGATT TCCCAGACAC ATTTCCCACA 
GATCAACCGG ATCGCTTTGA TCTGATGTGC CAGTACTTGG GCCTGGACCC CATGCTGGGT 
GTCTACTTTG ACATGGAGAC GGTATACGCC ACCATGATGC ACTCGTTTGC CTCGCATCCT 
CACATTGACC GTGAAGTGGA ACAGCGTTGT CAGCCTGACG CCCGTCGTTC CCTTCAGGTG 
GAACCTTGCC TGCGCCCGCT GCCCCGCTTG AAGGTACTGT GTGATGACTT CAGCGATTTG 
ATCAACAGCG TCTCGGACAT CTTCTGTCCG AACAACGAGC GCGAAGAGAT GAAGACACCA 
ACGATGTGTC TGATATGCGG CTTAATCCTG TGCGGTCAGT CGTACTGCTG CCAGCCAGAG 
CTGGGCAAGG TCTCGGTGGG CGCATGCACT CATCACGCTC ACGCTTGTGG CGCCGAAGTG 
GGCATCTTTC TGCGCATTCG CGACTGCCAG GTGGTGTACC TGGGCCGTGG CAAGGGGTGC 
TTCGTCCCGC CGCCATATTT GGACGAGTAC GGCGAGACGG ATATGGGTCT GAGGCGCGGT 
AATCCGCTGA GACTGTCCCA GGCGGCTTAC AGAAAGATCT ACCTGCAGTG GCTGGGGCAT 
GGGCTGCACG GCGAGATCGC CCGTCTGAAC GACAACGCCA ATGTGGCAGC GGCGGCGCAG 
TGGCATCACA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ubr1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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