eIF4EHP cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF4EHP
Entrez Gene ID	326255
Full Name	eIF4E-Homologous Protein
Synonyms	4E-HP,CG10716,CG33100,CT30033,Dmel\CG33100,d4EHP,eIF4E-8
General protein information	Preferred Names eIF4E-Homologous Protein Names CG33100 gene product from transcript CG33100-RA 4EHP-PA 4EHP-PB 4EHP-PC 4EHP-PD CG33100-PA CG33100-PB CG33100-PC CG33100-PD eIF4E-Homologous protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95D11-95E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001202346.2	NP_001189275.1	eukaryotic translation initiation factor 4E homologous protein, isoform D
NM_176552.3	NP_788729.1	eukaryotic translation initiation factor 4E homologous protein, isoform A
NM_001202345.2	NP_001189274.1	eukaryotic translation initiation factor 4E homologous protein, isoform C
NM_001170239.3	NP_001163710.1	eukaryotic translation initiation factor 4E homologous protein, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	eif4e2rs1	NP_957053.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002948	XP_311951.4
Oryza sativa (rice)	Os03g0262400	NP_001049628.1
Xenopus tropicalis (tropical clawed frog)	eif4e2	NP_001016076.1
Drosophila melanogaster (fruit fly)	4EHP	NP_001189275.1
Arabidopsis thaliana (thale cress)	NCBP	NP_197312.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Consistent with this, 4EHP is highly expressed in the prothoracic gland and it is required for wild type expression levels of steroidogenic enzymes
Title: Drosophila 4EHP is essential for the larval-pupal transition and required in the prothoracic gland for ecdysone biosynthesis.

findings show that the belle RNA is translationally repressed by the d4EHP protein in the Drosophila ovary.
Title: Patterning of the Drosophila oocyte by a sequential translation repression program involving the d4EHP and Belle translational repressors.

By regulating Cad and Hb expression, d4EHP plays a key role in establishing anterior-posterior axis polarity in the Drosophila embryo.
Title: Cap-dependent translational inhibition establishes two opposing morphogen gradients in Drosophila embryos.

The following eIF4EHP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF4EHP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07246	NM_176552.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08027	NM_001202346.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08028	NM_001170239.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08029	NM_001202345.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF4EHP ( NM_176552.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788729.1 Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa07246
Clone ID Related Accession (Same CDS sequence)	NM_176552.3
Accession Version	NM_176552.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4E homologous protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_176552.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATGG AGAAAGTAGC CAACAAGCAG TACGAGACGA AAAACTGGCC AGATATTGTC 
GACAGCGACG ACAGCGATGT GGATAATCAG ATAGATGTGG ACAACCTGCC ACCACTGGAG 
GTGGGTCCCG GCGAGAACCG GCTGCAGCAC ACATACTGCC TCTGGTTCTC TCGCAAGGAG 
ACGCAGCGCG CGGCCGCCGA CTACAGCAAG TCGCTGCACA TGGTCGGCCG GTGCGCCAGC 
GTGCAGCAGT GGTGGTCGCT CTACTCGCAC CTCATCCGGC CCACCGCCCT GAAGCCCTAC 
CGGGAGCTCC TCCTCTTCAA GCAGGGCATC ATACCGATGT GGGAGGACCC GGCGAACAGC 
AAGGGCGGCC AGTGGTTGAT ACGACTACGC AAGAACAAGG TCGACCGGGC CTGGGAGAAC 
GTTTGTATGG CGATGCTCGG GGAGCAGTTC CTCGTCGGCG ACGAGATATG CGGAGTCGTG 
CTACAGACGA AATATCCGGA GGATAGCTTA TCAGTATGGC ACCGGACTGC CACTGATATG 
ACCAGTACAA CACGTATCCG TGATACGTTA CGCAGGATAT TAAATATACC TCTAACAACG 
GCATTGGAGT ATAAGATACA CTGTGATAGC CTTAAATATG TTTCAATTAA TAAAGGCCCA 
TTGAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788729.1
CDS	454..1125
Translation	MSMEKVANKQYETKNWPDIVDSDDSDVDNQIDVDNLPPLEVGPGENRLQHTYCLWFSRKETQRAAADYSKSLHMVGRCASVQQWWSLYSHLIRPTALKPYRELLLFKQGIIPMWEDPANSKGGQWLIRLRKNKVDRAWENVCMAMLGEQFLVGDEICGVVLQTKYPEDSLSVWHRTATDMTSTTRIRDTLRRILNIPLTTALEYKIHCDSLKYVSINKGPLKS

Target ORF information:

RefSeq Version	NM_176552.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176552.3

ORF Insert Sequence:

ATGAGCATGG AGAAAGTAGC CAACAAGCAG TACGAGACGA AAAACTGGCC AGATATTGTC 
GACAGCGACG ACAGCGATGT GGATAATCAG ATAGATGTGG ACAACCTGCC ACCACTGGAG 
GTGGGTCCCG GCGAGAACCG GCTGCAGCAC ACATACTGCC TCTGGTTCTC TCGCAAGGAG 
ACGCAGCGCG CGGCCGCCGA CTACAGCAAG TCGCTGCACA TGGTCGGCCG GTGCGCCAGC 
GTGCAGCAGT GGTGGTCGCT CTACTCGCAC CTCATCCGGC CCACCGCCCT GAAGCCCTAC 
CGGGAGCTCC TCCTCTTCAA GCAGGGCATC ATACCGATGT GGGAGGACCC GGCGAACAGC 
AAGGGCGGCC AGTGGTTGAT ACGACTACGC AAGAACAAGG TCGACCGGGC CTGGGAGAAC 
GTTTGTATGG CGATGCTCGG GGAGCAGTTC CTCGTCGGCG ACGAGATATG CGGAGTCGTG 
CTACAGACGA AATATCCGGA GGATAGCTTA TCAGTATGGC ACCGGACTGC CACTGATATG 
ACCAGTACAA CACGTATCCG TGATACGTTA CGCAGGATAT TAAATATACC TCTAACAACG 
GCATTGGAGT ATAAGATACA CTGTGATAGC CTTAAATATG TTTCAATTAA TAAAGGCCCA 
TTGAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4EHP ( NM_001202346.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189275.1 Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa08027
Clone ID Related Accession (Same CDS sequence)	NM_001202346.2
Accession Version	NM_001202346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4E homologous protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001202346.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATGG AGAAAGTAGC CAACAAGCAG TACGAGACGA AAAACTGGCC AGATATTGTC 
GACAGCGACG ACAGCGATGT GGATAATCAG ATAGATGTGG ACAACCTGCC ACCACTGGAG 
GTGGGTCCCG GCGAGAACCG GCTGCAGCAC ACATACTGCC TCTGGTTCTC TCGCAAGGAG 
ACGCAGCGCG CGGCCGCCGA CTACAGCAAG TCGCTGCACA TGGTCGGCCG GTGCGCCAGC 
GTGCAGCAGT GGTGGTCGCT CTACTCGCAC CTCATCCGGC CCACCGCCCT GAAGCCCTAC 
CGGGAGCTCC TCCTCTTCAA GCAGGGCATC ATACCGATGT GGGAGGACCC GGCGAACAGC 
AAGGGCGGCC AGTGGTTGAT ACGACTACGC AAGAACAAGG TCGACCGGGC CTGGGAGAAC 
GTTTGTATGG CGATGCTCGG GGAGCAGTTC CTCGTCGGCG ACGAGATATG CGGAGTCGTG 
CTACAGACGA AATATCCGGA GGATAGCTTA TCAGTATGGC ACCGGACTGC CACTGATATG 
ACCAGTACAA CACGTATCCG TGATACGTTA CGCAGGATAT TAAATATACC TCTAACAACG 
GCATTGGAGT ATAAGATACA CTGTGATAGC CTTAAATATG TTTCAATGCC GAGACGTCAA 
AATCATAAGC TGGGAAACCT TTTTTATCGA AATCGATACG GCTTTAATTC AAGATACGGA 
AAATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189275.1
CDS	454..1182
Translation	MSMEKVANKQYETKNWPDIVDSDDSDVDNQIDVDNLPPLEVGPGENRLQHTYCLWFSRKETQRAAADYSKSLHMVGRCASVQQWWSLYSHLIRPTALKPYRELLLFKQGIIPMWEDPANSKGGQWLIRLRKNKVDRAWENVCMAMLGEQFLVGDEICGVVLQTKYPEDSLSVWHRTATDMTSTTRIRDTLRRILNIPLTTALEYKIHCDSLKYVSMPRRQNHKLGNLFYRNRYGFNSRYGKS

Target ORF information:

RefSeq Version	NM_001202346.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202346.2

ORF Insert Sequence:

ATGAGCATGG AGAAAGTAGC CAACAAGCAG TACGAGACGA AAAACTGGCC AGATATTGTC 
GACAGCGACG ACAGCGATGT GGATAATCAG ATAGATGTGG ACAACCTGCC ACCACTGGAG 
GTGGGTCCCG GCGAGAACCG GCTGCAGCAC ACATACTGCC TCTGGTTCTC TCGCAAGGAG 
ACGCAGCGCG CGGCCGCCGA CTACAGCAAG TCGCTGCACA TGGTCGGCCG GTGCGCCAGC 
GTGCAGCAGT GGTGGTCGCT CTACTCGCAC CTCATCCGGC CCACCGCCCT GAAGCCCTAC 
CGGGAGCTCC TCCTCTTCAA GCAGGGCATC ATACCGATGT GGGAGGACCC GGCGAACAGC 
AAGGGCGGCC AGTGGTTGAT ACGACTACGC AAGAACAAGG TCGACCGGGC CTGGGAGAAC 
GTTTGTATGG CGATGCTCGG GGAGCAGTTC CTCGTCGGCG ACGAGATATG CGGAGTCGTG 
CTACAGACGA AATATCCGGA GGATAGCTTA TCAGTATGGC ACCGGACTGC CACTGATATG 
ACCAGTACAA CACGTATCCG TGATACGTTA CGCAGGATAT TAAATATACC TCTAACAACG 
GCATTGGAGT ATAAGATACA CTGTGATAGC CTTAAATATG TTTCAATGCC GAGACGTCAA 
AATCATAAGC TGGGAAACCT TTTTTATCGA AATCGATACG GCTTTAATTC AAGATACGGA 
AAATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4EHP ( NM_001170239.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163710.1 Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08028
Clone ID Related Accession (Same CDS sequence)	NM_001170239.3
Accession Version	NM_001170239.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4E homologous protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170239.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATGG AGAAAGTAGC CAACAAGCAG TACGAGACGA AAAACTGGCC AGATATTGTC 
GACAGCGACG ACAGCGATGT GGATAATCAG ATAGATGTGG ACAACCTGCC ACCACTGGAG 
GTGGGTCCCG GCGAGAACCG GCTGCAGCAC ACATACTGCC TCTGGTTCTC TCGCAAGGAG 
ACGCAGCGCG CGGCCGCCGA CTACAGCAAG TCGCTGCACA TGGTCGGCCG GTGCGCCAGC 
GTGCAGCAGT GGTGGTCGCT CTACTCGCAC CTCATCCGGC CCACCGCCCT GAAGCCCTAC 
CGGGAGCTCC TCCTCTTCAA GCAGGGCATC ATACCGATGT GGGAGGACCC GGCGAACAGC 
AAGGGCGGCC AGTGGTTGAT ACGACTACGC AAGAACAAGG TCGACCGGGC CTGGGAGAAC 
GTTTGTATGG CGATGCTCGG GGAGCAGTTC CTCGTCGGCG ACGAGATATG CGGAGTCGTG 
CTACAGACGA AATATCCGAA TCCAAGCATC CAAGTAGCAT GCTCATCAAG GCCTATAATC 
TGCATTATCT ACACACGTTT TGTAGTACAA TTCGGAAAAT GCAAAATCAT TATAGTTACT 
AATAACAATA AACGCAATGT GTATGAGTTT TTGACAATTA TTGTGATTAA GATACACGAA 
GAAATGCTTT CGCTTCTAAG TAGGAAATGC AAATTTGTTA ATGCACCAAA CGAACTGGTC 
ACGCGAATTG GTCGATTTCT GTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163710.1
CDS	454..1200
Translation	MSMEKVANKQYETKNWPDIVDSDDSDVDNQIDVDNLPPLEVGPGENRLQHTYCLWFSRKETQRAAADYSKSLHMVGRCASVQQWWSLYSHLIRPTALKPYRELLLFKQGIIPMWEDPANSKGGQWLIRLRKNKVDRAWENVCMAMLGEQFLVGDEICGVVLQTKYPNPSIQVACSSRPIICIIYTRFVVQFGKCKIIIVTNNNKRNVYEFLTIIVIKIHEEMLSLLSRKCKFVNAPNELVTRIGRFLF

Target ORF information:

RefSeq Version	NM_001170239.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170239.3

ORF Insert Sequence:

ATGAGCATGG AGAAAGTAGC CAACAAGCAG TACGAGACGA AAAACTGGCC AGATATTGTC 
GACAGCGACG ACAGCGATGT GGATAATCAG ATAGATGTGG ACAACCTGCC ACCACTGGAG 
GTGGGTCCCG GCGAGAACCG GCTGCAGCAC ACATACTGCC TCTGGTTCTC TCGCAAGGAG 
ACGCAGCGCG CGGCCGCCGA CTACAGCAAG TCGCTGCACA TGGTCGGCCG GTGCGCCAGC 
GTGCAGCAGT GGTGGTCGCT CTACTCGCAC CTCATCCGGC CCACCGCCCT GAAGCCCTAC 
CGGGAGCTCC TCCTCTTCAA GCAGGGCATC ATACCGATGT GGGAGGACCC GGCGAACAGC 
AAGGGCGGCC AGTGGTTGAT ACGACTACGC AAGAACAAGG TCGACCGGGC CTGGGAGAAC 
GTTTGTATGG CGATGCTCGG GGAGCAGTTC CTCGTCGGCG ACGAGATATG CGGAGTCGTG 
CTACAGACGA AATATCCGAA TCCAAGCATC CAAGTAGCAT GCTCATCAAG GCCTATAATC 
TGCATTATCT ACACACGTTT TGTAGTACAA TTCGGAAAAT GCAAAATCAT TATAGTTACT 
AATAACAATA AACGCAATGT GTATGAGTTT TTGACAATTA TTGTGATTAA GATACACGAA 
GAAATGCTTT CGCTTCTAAG TAGGAAATGC AAATTTGTTA ATGCACCAAA CGAACTGGTC 
ACGCGAATTG GTCGATTTCT GTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4EHP ( NM_001202345.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189274.1 Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08029
Clone ID Related Accession (Same CDS sequence)	NM_001202345.2
Accession Version	NM_001202345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4E homologous protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001202345.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATGG AGAAAGTAGC CAACAAGCAG TACGAGACGA AAAACTGGCC AGATATTGTC 
GACAGCGACG ACAGCGATGT GGATAATCAG ATAGATGTGG ACAACCTGCC ACCACTGGAG 
GTGGGTCCCG GCGAGAACCG GCTGCAGCAC ACATACTGCC TCTGGTTCTC TCGCAAGGAG 
ACGCAGCGCG CGGCCGCCGA CTACAGCAAG TCGCTGCACA TGGTCGGCCG GTGCGCCAGC 
GTGCAGCAGT GGTGGTCGCT CTACTCGCAC CTCATCCGGC CCACCGCCCT GAAGCCCTAC 
CGGGAGCTCC TCCTCTTCAA GCAGGGCATC ATACCGATGT GGGAGGACCC GGCGAACAGC 
AAGGGCGGCC AGTGGTTGAT ACGACTACGC AAGAACAAGG TCGACCGGGC CTGGGAGAAC 
GTTTGTATGG CGATGCTCGG GGAGCAGTTC CTCGTCGGCG ACGAGATATG CGGAGTCGTG 
CTACAGACGA AATATCCGAT AAAAACCGAA CACAGCCGGC CAATGCATAC AAATGAGCGA 
AGGAGCGATG GAGTCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189274.1
CDS	454..1014
Translation	MSMEKVANKQYETKNWPDIVDSDDSDVDNQIDVDNLPPLEVGPGENRLQHTYCLWFSRKETQRAAADYSKSLHMVGRCASVQQWWSLYSHLIRPTALKPYRELLLFKQGIIPMWEDPANSKGGQWLIRLRKNKVDRAWENVCMAMLGEQFLVGDEICGVVLQTKYPIKTEHSRPMHTNERRSDGVT

Target ORF information:

RefSeq Version	NM_001202345.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4E homologous protein (eIF4EHP), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202345.2

ORF Insert Sequence:

ATGAGCATGG AGAAAGTAGC CAACAAGCAG TACGAGACGA AAAACTGGCC AGATATTGTC 
GACAGCGACG ACAGCGATGT GGATAATCAG ATAGATGTGG ACAACCTGCC ACCACTGGAG 
GTGGGTCCCG GCGAGAACCG GCTGCAGCAC ACATACTGCC TCTGGTTCTC TCGCAAGGAG 
ACGCAGCGCG CGGCCGCCGA CTACAGCAAG TCGCTGCACA TGGTCGGCCG GTGCGCCAGC 
GTGCAGCAGT GGTGGTCGCT CTACTCGCAC CTCATCCGGC CCACCGCCCT GAAGCCCTAC 
CGGGAGCTCC TCCTCTTCAA GCAGGGCATC ATACCGATGT GGGAGGACCC GGCGAACAGC 
AAGGGCGGCC AGTGGTTGAT ACGACTACGC AAGAACAAGG TCGACCGGGC CTGGGAGAAC 
GTTTGTATGG CGATGCTCGG GGAGCAGTTC CTCGTCGGCG ACGAGATATG CGGAGTCGTG 
CTACAGACGA AATATCCGAT AAAAACCGAA CACAGCCGGC CAATGCATAC AAATGAGCGA 
AGGAGCGATG GAGTCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eIF4EHP cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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