rumi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rumi
Entrez Gene ID	326122
Full Name	CG31152 gene product from transcript CG31152-RB
Synonyms	CG17138,CG31152,Dmel\CG31152,Rumi
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94C4-94C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_142838.4	NP_651095.1	rumi, isoform A
NM_001275920.2	NP_001262849.1	rumi, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	POGLUT1	NP_689518.1
	POGLUT1	XP_001109371.1
Rattus norvegicus (Norway rat)	Poglut1	NP_001094122.1
Oryza sativa (rice)	Os02g0642700	NP_001047557.2
Arabidopsis thaliana (thale cress)	AT5G23850	NP_197774.1
Canis lupus familiaris (dog)	POGLUT1	XP_545115.3
Gallus gallus (chicken)	POGLUT1	NP_001012560.1
Arabidopsis thaliana (thale cress)	AT3G48980	NP_190467.1
Bos taurus (cattle)	POGLUT1	NP_001014903.1
Danio rerio (zebrafish)	poglut1	NP_001032511.1
Anopheles gambiae (African malaria mosquito)	CAP10_ANOGA	XP_001230897.1
Pan troglodytes (chimpanzee)	POGLUT1	XP_516666.2
Mus musculus (house mouse)	Poglut1	NP_759012.1
Drosophila melanogaster (fruit fly)	rumi	NP_651095.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study suggests that loss of Rumi activity may underlie these diseases, and the mechanistic insights may facilitate the development of modulators of Notch signaling.
Title: Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations.

our data suggest that O-glucosylation of Eys by Rumi ensures rhabdomere separation by promoting proper Eys folding and stability in a critical time window during the mid-pupal stage.
Title: The protein O-glucosyltransferase Rumi modifies eyes shut to promote rhabdomere separation in Drosophila.

Rumi functions as both a protein O-glucosyltransferase and a protein O-xylosyltransferase.
Title: Rumi functions as both a protein O-glucosyltransferase and a protein O-xylosyltransferase.

rumi fully suppresses the gain-of-function phenotype of a ligand-independent mutant form of Notch
Title: Multiple O-glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling.

Rumi is a CAP10 domain glycerosyltransferase that modifies Notch and is required for Notch signaling.
Title: Rumi is a CAP10 domain glycosyltransferase that modifies Notch and is required for Notch signaling.

The following rumi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rumi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07210	NM_142838.4 Latest version!	Drosophila melanogaster rumi, transcript variant A (rumi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08591	NM_001275920.2 Latest version!	Drosophila melanogaster rumi, transcript variant B (rumi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rumi ( NM_142838.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651095.1 Drosophila melanogaster rumi, transcript variant A (rumi), mRNA.

Price & Availability↑

CloneID	OFa07210
Clone ID Related Accession (Same CDS sequence)	NM_142838.4
Accession Version	NM_142838.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rumi, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142838.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTAATAA ATCATTTAAT AGTCGTATTA TTAATTAGTT TGGTAGGAAC AGGAGGTGCA 
GAAGATGACG GACTTTGCAG TGCCGATCAA AAATCCTGTG CACAAAGTGA ACCCGACCAA 
ATAAACGAGG ATGAATTTAG CTTCAAGATA CGTCGCCAGA TCGAAAAAGC CAATGCCGAC 
TACAAGCCGT GCAGCAGTGA TCCCCAGGAC AGCGACTGCT CCTGCCACGC AAATGTTCTG 
AAACGGGATC TCGCGCCCTA CAAGTCAACA GGTGTCACCC GGCAGATGAT CGAGAGTTCA 
GCGCGTTACG GCACCAAATA CAAGATCTAC GGACATCGCC TGTACCGGGA TGCCAACTGC 
ATGTTCCCAG CCCGCTGTGA GGGCATTGAA CACTTCCTGC TGCCGCTGGT GGCTACGCTG 
CCCGACATGG ACTTGATCAT CAATACCAGG GACTATCCGC AGCTGAATGC GGCGTGGGGT 
AACGCGGCTG GTGGTCCCGT CTTCTCCTTC TCCAAGACAA AGGAGTACCG CGACATCATG 
TATCCAGCGT GGACATTCTG GGCCGGAGGA CCGGCCACCA AGCTCCATCC TCGTGGCATT 
GGACGCTGGG ATCAGATGCG CGAGAAGCTG GAGAAGCGAG CGGCAGCTAT ACCCTGGTCA 
CAGAAACGCA GTCTGGGCTT CTTTCGCGGT TCACGCACCT CGGACGAACG TGATTCTCTT 
ATTCTGCTCT CGCGCCGCAA TCCGGAGCTA GTCGAGGCTC AATACACCAA AAATCAGGGC 
TGGAAGTCGC CTAAGGATAC ACTGGACGCA CCGGCTGCTG ATGAGGTGTC CTTCGAGGAC 
CACTGCAAGT ACAAGTACTT GTTCAATTTC CGCGGTGTGG CCGCCAGTTT TCGTCTGAAG 
CACTTGTTCC TCTGCAAGTC GCTGGTCTTC CATGTGGGCG ACGAGTGGCA GGAGTTCTTC 
TACGACCAAC TAAAGCCCTG GGTGCACTAT GTGCCGCTCA AGAGCTACCC CAGTCAGCAG 
GAGTACGAGC ACATCTTGTC CTTTTTCAAG AAAAACGATG CGCTGGCCCA GGAGATCGCT 
CAGCGTGGAT ACGACTTCAT CTGGGAGCAT TTGCGCATGA AGGACATCAA GTGCTATTGG 
CGCAAGCTGC TCAAGCGATA TGTGAAGCTG CTGCAATACG AGGTTAAGCC GGAGGATCAA 
CTTATTTACA TAGGGCCTAA AAAGGATGAG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651095.1
CDS	74..1309
Translation	MLINHLIVVLLISLVGTGGAEDDGLCSADQKSCAQSEPDQINEDEFSFKIRRQIEKANADYKPCSSDPQDSDCSCHANVLKRDLAPYKSTGVTRQMIESSARYGTKYKIYGHRLYRDANCMFPARCEGIEHFLLPLVATLPDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTFWAGGPATKLHPRGIGRWDQMREKLEKRAAAIPWSQKRSLGFFRGSRTSDERDSLILLSRRNPELVEAQYTKNQGWKSPKDTLDAPAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLCKSLVFHVGDEWQEFFYDQLKPWVHYVPLKSYPSQQEYEHILSFFKKNDALAQEIAQRGYDFIWEHLRMKDIKCYWRKLLKRYVKLLQYEVKPEDQLIYIGPKKDEL

Target ORF information:

RefSeq Version	NM_142838.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rumi, transcript variant A (rumi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142838.4

ORF Insert Sequence:

ATGCTAATAA ATCATTTAAT AGTCGTATTA TTAATTAGTT TGGTAGGAAC AGGAGGTGCA 
GAAGATGACG GACTTTGCAG TGCCGATCAA AAATCCTGTG CACAAAGTGA ACCCGACCAA 
ATAAACGAGG ATGAATTTAG CTTCAAGATA CGTCGCCAGA TCGAAAAAGC CAATGCCGAC 
TACAAGCCGT GCAGCAGTGA TCCCCAGGAC AGCGACTGCT CCTGCCACGC AAATGTTCTG 
AAACGGGATC TCGCGCCCTA CAAGTCAACA GGTGTCACCC GGCAGATGAT CGAGAGTTCA 
GCGCGTTACG GCACCAAATA CAAGATCTAC GGACATCGCC TGTACCGGGA TGCCAACTGC 
ATGTTCCCAG CCCGCTGTGA GGGCATTGAA CACTTCCTGC TGCCGCTGGT GGCTACGCTG 
CCCGACATGG ACTTGATCAT CAATACCAGG GACTATCCGC AGCTGAATGC GGCGTGGGGT 
AACGCGGCTG GTGGTCCCGT CTTCTCCTTC TCCAAGACAA AGGAGTACCG CGACATCATG 
TATCCAGCGT GGACATTCTG GGCCGGAGGA CCGGCCACCA AGCTCCATCC TCGTGGCATT 
GGACGCTGGG ATCAGATGCG CGAGAAGCTG GAGAAGCGAG CGGCAGCTAT ACCCTGGTCA 
CAGAAACGCA GTCTGGGCTT CTTTCGCGGT TCACGCACCT CGGACGAACG TGATTCTCTT 
ATTCTGCTCT CGCGCCGCAA TCCGGAGCTA GTCGAGGCTC AATACACCAA AAATCAGGGC 
TGGAAGTCGC CTAAGGATAC ACTGGACGCA CCGGCTGCTG ATGAGGTGTC CTTCGAGGAC 
CACTGCAAGT ACAAGTACTT GTTCAATTTC CGCGGTGTGG CCGCCAGTTT TCGTCTGAAG 
CACTTGTTCC TCTGCAAGTC GCTGGTCTTC CATGTGGGCG ACGAGTGGCA GGAGTTCTTC 
TACGACCAAC TAAAGCCCTG GGTGCACTAT GTGCCGCTCA AGAGCTACCC CAGTCAGCAG 
GAGTACGAGC ACATCTTGTC CTTTTTCAAG AAAAACGATG CGCTGGCCCA GGAGATCGCT 
CAGCGTGGAT ACGACTTCAT CTGGGAGCAT TTGCGCATGA AGGACATCAA GTGCTATTGG 
CGCAAGCTGC TCAAGCGATA TGTGAAGCTG CTGCAATACG AGGTTAAGCC GGAGGATCAA 
CTTATTTACA TAGGGCCTAA AAAGGATGAG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rumi ( NM_001275920.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262849.1 Drosophila melanogaster rumi, transcript variant B (rumi), mRNA.

Price & Availability↑

CloneID	OFa08591
Clone ID Related Accession (Same CDS sequence)	NM_001275920.2
Accession Version	NM_001275920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rumi, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275920.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCGAGA GTTCAGCGCG TTACGGCACC AAATACAAGA TCTACGGACA TCGCCTGTAC 
CGGGATGCCA ACTGCATGTT CCCAGCCCGC TGTGAGGGCA TTGAACACTT CCTGCTGCCG 
CTGGTGGCTA CGCTGCCCGA CATGGACTTG ATCATCAATA CCAGGGACTA TCCGCAGCTG 
AATGCGGCGT GGGGTAACGC GGCTGGTGGT CCCGTCTTCT CCTTCTCCAA GACAAAGGAG 
TACCGCGACA TCATGTATCC AGCGTGGACA TTCTGGGCCG GAGGACCGGC CACCAAGCTC 
CATCCTCGTG GCATTGGACG CTGGGATCAG ATGCGCGAGA AGCTGGAGAA GCGAGCGGCA 
GCTATACCCT GGTCACAGAA ACGCAGTCTG GGCTTCTTTC GCGGTTCACG CACCTCGGAC 
GAACGTGATT CTCTTATTCT GCTCTCGCGC CGCAATCCGG AGCTAGTCGA GGCTCAATAC 
ACCAAAAATC AGGGCTGGAA GTCGCCTAAG GATACACTGG ACGCACCGGC TGCTGATGAG 
GTGTCCTTCG AGGACCACTG CAAGTACAAG TACTTGTTCA ATTTCCGCGG TGTGGCCGCC 
AGTTTTCGTC TGAAGCACTT GTTCCTCTGC AAGTCGCTGG TCTTCCATGT GGGCGACGAG 
TGGCAGGAGT TCTTCTACGA CCAACTAAAG CCCTGGGTGC ACTATGTGCC GCTCAAGAGC 
TACCCCAGTC AGCAGGAGTA CGAGCACATC TTGTCCTTTT TCAAGAAAAA CGATGCGCTG 
GCCCAGGAGA TCGCTCAGCG TGGATACGAC TTCATCTGGG AGCATTTGCG CATGAAGGAC 
ATCAAGTGCT ATTGGCGCAA GCTGCTCAAG CGATATGTGA AGCTGCTGCA ATACGAGGTT 
AAGCCGGAGG ATCAACTTAT TTACATAGGG CCTAAAAAGG ATGAGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262849.1
CDS	355..1305
Translation	MIESSARYGTKYKIYGHRLYRDANCMFPARCEGIEHFLLPLVATLPDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTFWAGGPATKLHPRGIGRWDQMREKLEKRAAAIPWSQKRSLGFFRGSRTSDERDSLILLSRRNPELVEAQYTKNQGWKSPKDTLDAPAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLCKSLVFHVGDEWQEFFYDQLKPWVHYVPLKSYPSQQEYEHILSFFKKNDALAQEIAQRGYDFIWEHLRMKDIKCYWRKLLKRYVKLLQYEVKPEDQLIYIGPKKDEL

Target ORF information:

RefSeq Version	NM_001275920.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rumi, transcript variant B (rumi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275920.2

ORF Insert Sequence:

ATGATCGAGA GTTCAGCGCG TTACGGCACC AAATACAAGA TCTACGGACA TCGCCTGTAC 
CGGGATGCCA ACTGCATGTT CCCAGCCCGC TGTGAGGGCA TTGAACACTT CCTGCTGCCG 
CTGGTGGCTA CGCTGCCCGA CATGGACTTG ATCATCAATA CCAGGGACTA TCCGCAGCTG 
AATGCGGCGT GGGGTAACGC GGCTGGTGGT CCCGTCTTCT CCTTCTCCAA GACAAAGGAG 
TACCGCGACA TCATGTATCC AGCGTGGACA TTCTGGGCCG GAGGACCGGC CACCAAGCTC 
CATCCTCGTG GCATTGGACG CTGGGATCAG ATGCGCGAGA AGCTGGAGAA GCGAGCGGCA 
GCTATACCCT GGTCACAGAA ACGCAGTCTG GGCTTCTTTC GCGGTTCACG CACCTCGGAC 
GAACGTGATT CTCTTATTCT GCTCTCGCGC CGCAATCCGG AGCTAGTCGA GGCTCAATAC 
ACCAAAAATC AGGGCTGGAA GTCGCCTAAG GATACACTGG ACGCACCGGC TGCTGATGAG 
GTGTCCTTCG AGGACCACTG CAAGTACAAG TACTTGTTCA ATTTCCGCGG TGTGGCCGCC 
AGTTTTCGTC TGAAGCACTT GTTCCTCTGC AAGTCGCTGG TCTTCCATGT GGGCGACGAG 
TGGCAGGAGT TCTTCTACGA CCAACTAAAG CCCTGGGTGC ACTATGTGCC GCTCAAGAGC 
TACCCCAGTC AGCAGGAGTA CGAGCACATC TTGTCCTTTT TCAAGAAAAA CGATGCGCTG 
GCCCAGGAGA TCGCTCAGCG TGGATACGAC TTCATCTGGG AGCATTTGCG CATGAAGGAC 
ATCAAGTGCT ATTGGCGCAA GCTGCTCAAG CGATATGTGA AGCTGCTGCA ATACGAGGTT 
AAGCCGGAGG ATCAACTTAT TTACATAGGG CCTAAAAAGG ATGAGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rumi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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