Stim cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stim
Entrez Gene ID	32556
Full Name	Stromal interaction molecule
Synonyms	C-Stim,CG9126,D-STIM,D-Stim,Dmel\CG9126,STIM,STIM1,dSTIM,dStim,dstim,stim1
General protein information	Preferred Names Stromal interaction molecule Names CG9126 gene product from transcript CG9126-RD CG9126-PA CG9126-PD CG9126-PF CG9126-PG Stim-PA Stim-PD Stim-PF Stim-PG drosophila stromal interaction molecule stromal interaction molecule stromal interaction molecule 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14A1-14A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_206747.3	NP_996470.2	stromal interaction molecule, isoform D
NM_001258774.2	NP_001245703.1	stromal interaction molecule, isoform F
NM_078633.4	NP_523357.2	stromal interaction molecule, isoform A
NM_001298380.1	NP_001285309.1	stromal interaction molecule, isoform G

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	stim-1	NP_741073.1
Danio rerio (zebrafish)	stim1a	NP_001038264.1
Xenopus tropicalis (tropical clawed frog)	stim1	NP_991397.1
Pan troglodytes (chimpanzee)	STIM1	XP_003951854.1
Bos taurus (cattle)	STIM1	NP_001030486.1
Mus musculus (house mouse)	Stim1	NP_033313.2
Rattus norvegicus (Norway rat)	Stim1	NP_001101966.2
Drosophila melanogaster (fruit fly)	Stim	NP_523357.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000175	XP_310961.4
Homo sapiens (human)	STIM1	NP_001264890.1
	STIM1	XP_001112949.1
Canis lupus familiaris (dog)	STIM1	XP_005633633.1
Gallus gallus (chicken)	STIM1	NP_001026009.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

after depletion of Ca(2+) stores in Drosophila, translocation of the IP3R to Endoplasmic reticulum-plasma-membrane junctions facilitates the coupling of STIM to Orai that leads to activation of Store-operated Ca(2+) entry.
Title: Mutant IP3 receptors attenuate store-operated Ca2+ entry by destabilizing STIM-Orai interactions in Drosophila neurons.

Data indicate that STIM functions as sensors of Ca2+ stored in the endoplasmic reticulum (ER) lumen and translocate into ER-PM (plasma membrane) junctions to tether and activate Orai channels when ER Ca2+ concentration decreases.
Title: Orai channel pore properties and gating by STIM: implications from the Orai crystal structure.

Induced STIM1 translocation to the plasma membrane promotes intestinal epithelial cell migration after wounding by enhancing TRPC1-mediated Ca2+ signaling and provide new insight into the mechanism of intestinal epithelial restitution.
Title: STIM1 translocation to the plasma membrane enhances intestinal epithelial restitution by inducing TRPC1-mediated Ca2+ signaling after wounding.

Interaction with the C terminus of Stim thus induces Orai dimers to dimerize, forming tetramers that constitute the Ca(2+)-selective pore.
Title: The CRAC channel consists of a tetramer formed by Stim-induced dimerization of Orai dimers.

dSTIM regulates growth and patterning of imaginal discs and indicate potential interactions with the Notch and Wingless signaling pathways.
Title: The Drosophila STIM1 orthologue, dSTIM, has roles in cell fate specification and tissue patterning.

The following Stim gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stim cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01734	NM_206747.3 Latest version!	Drosophila melanogaster stromal interaction molecule, transcript variant D (Stim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01735	NM_078633.4 Latest version!	Drosophila melanogaster stromal interaction molecule, transcript variant A (Stim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa03623	NM_001298380.1 Latest version!	Drosophila melanogaster stromal interaction molecule, transcript variant G (Stim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa03623	NM_001258774.2 Latest version!	Drosophila melanogaster stromal interaction molecule, transcript variant F (Stim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stim ( NM_206747.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996470.2 Drosophila melanogaster stromal interaction molecule, transcript variant D (Stim), mRNA.

Price & Availability↑

CloneID	OFa01734
Clone ID Related Accession (Same CDS sequence)	NM_206747.3
Accession Version	NM_206747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stromal interaction molecule, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206747.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAAAGA ATACCATTTG GAACTACTCT TTAATATTCT TCTGCTGTGT GCTGAAGAGC 
ATAAGTACGC TAGATCATGG CCCGCACACA GTATCAGTCG ATTCGAATCG ACACAACACA 
CAGCATCAGT ATAAGCAAAA TCCCAATGTT GCCTCACAAC GTCACTCATC CCACGAATCT 
GGTCAGAGTT TACACAATTC GCAATCGGAA CATGTCACCC ATATTGCCGC ATCGCACGCC 
GGAAGCGGCG GAGAGCACTC CACTCATTTG GCGCAAAATC TGCACAGGAG CTCATATAAT 
CTTCTGAGCG AGGCCATGTC CCAGGCTGTC AGCAATGAAT TTAGTTCCAT GGGAAGTGGT 
TCAGCGGATG GAGCGTGTGC TGCTGATGAT TTTGATTGCT ACAGTGGAAG TGTTCAGGAT 
CGCTTTGGCA TGGAGGCTAT TGCCAGCTTG CATCGTCAGC TAGATGATGA CGATAATGGA 
AACATCGATC TGAGCGAGTC CGATGACTTT TTGCGGGAGG AATTGAAGTA CGACTCGGGC 
TACGAAAAGC GGCAGAAAGC GTTTCACTTC AATGACGATA TGCATATATC GGTCAAAGAA 
CTTTGGGAGG CCTGGCTCAG ATCGGAGGTG CATAATTGGA CCATCGAGCA GACCACCGAT 
TGGCTGGCTC AGTCCGTTCA GCTGCCGCAA TACGTTGATC TGTTCAAATT ACACAAGGTT 
ACTGGCGCTG CCTTGCCAAG ATTGGCTGTG AATAATCTTC AGTATGTTGG CAATGTACTT 
GGCATCAAAG ACCCTATACA CAAACAAAAA ATCTCATTGA AGGCAATGGA TGTGGTTCTG 
TTTGGGCCAC CGCGAGAAAC TGGTACCCGC TGGAAAGACT ACATATTGGT AACACTGTTG 
CTTAGTGCTA TTATTGGTTG TTGGTACGCC TATCAGCAAA ATAAGAATGC CAAACGGCAT 
CTGCGTCGAA TGGCCCAGGA TATGGAGGGA TTGCAGAGGG CTGAGCAAAG TCTACAGGAG 
ATGCAGAAGG AACTAGAACG GGCCAGAATG GAGCAGGAAA ATGTGGCAAC AGAAAAACTA 
GATTTGGAGC GTCGTCTAAA AGAAGCGCCC ACTCTCAGTT CATCGAACTC GGATTTGGAA 
GTTCAGCAGC TGAAAAAGGA AATCGAGATG TTGCGCAACG AATTGTCCCG CGCCGAATTC 
GAGCTAGTAG ACAACTGCTG GTCACCGCCG CCACAACTGC AATCATGGCT TCAATACACA 
TATGAACTAG AAAGTAAGAA TCATCAGAAG AAGCGCACGT CGGCTGAGAA GCAGCTACAG 
TCGGCCAGAG AGGCTTGTGA GAAATTGCGT AAGAAACGGT CAAGTTTGGT GGGTGCGTTC 
GTTTCCACGC ACGGAAAGAG TATTGATGAT GTGGATCGGT CGATTGTTGA GGCACGGAAT 
GCCCTCGGAG ATGTAACAAA CGAGCTGCAA GAACGACTGC ATCGCTGGAA GCAAATCGAG 
ACGTGCCTTG GCTTAAACAT TGTGAACAAC AATGGTCTGC CCTACTTGGA GAATGTTCTG 
TACGGTCGAA ATGGGGGCTT ACAAAGTTCC ATGGGCATGA GTTCAACCAA GGGTTCTAGA 
GCACGTATTA CCAACAGCAC CGAAGACCTG GACGATGAGT CCATACAAGG TTTTGGAATT 
CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996470.2
CDS	193..1878
Translation	MRKNTIWNYSLIFFCCVLKSISTLDHGPHTVSVDSNRHNTQHQYKQNPNVASQRHSSHESGQSLHNSQSEHVTHIAASHAGSGGEHSTHLAQNLHRSSYNLLSEAMSQAVSNEFSSMGSGSADGACAADDFDCYSGSVQDRFGMEAIASLHRQLDDDDNGNIDLSESDDFLREELKYDSGYEKRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKLHKVTGAALPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKDYILVTLLLSAIIGCWYAYQQNKNAKRHLRRMAQDMEGLQRAEQSLQEMQKELERARMEQENVATEKLDLERRLKEAPTLSSSNSDLEVQQLKKEIEMLRNELSRAEFELVDNCWSPPPQLQSWLQYTYELESKNHQKKRTSAEKQLQSAREACEKLRKKRSSLVGAFVSTHGKSIDDVDRSIVEARNALGDVTNELQERLHRWKQIETCLGLNIVNNNGLPYLENVLYGRNGGLQSSMGMSSTKGSRARITNSTEDLDDESIQGFGIP

Target ORF information:

RefSeq Version	NM_206747.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stromal interaction molecule, transcript variant D (Stim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206747.3

ORF Insert Sequence:

ATGCGAAAGA ATACCATTTG GAACTACTCT TTAATATTCT TCTGCTGTGT GCTGAAGAGC 
ATAAGTACGC TAGATCATGG CCCGCACACA GTATCAGTCG ATTCGAATCG ACACAACACA 
CAGCATCAGT ATAAGCAAAA TCCCAATGTT GCCTCACAAC GTCACTCATC CCACGAATCT 
GGTCAGAGTT TACACAATTC GCAATCGGAA CATGTCACCC ATATTGCCGC ATCGCACGCC 
GGAAGCGGCG GAGAGCACTC CACTCATTTG GCGCAAAATC TGCACAGGAG CTCATATAAT 
CTTCTGAGCG AGGCCATGTC CCAGGCTGTC AGCAATGAAT TTAGTTCCAT GGGAAGTGGT 
TCAGCGGATG GAGCGTGTGC TGCTGATGAT TTTGATTGCT ACAGTGGAAG TGTTCAGGAT 
CGCTTTGGCA TGGAGGCTAT TGCCAGCTTG CATCGTCAGC TAGATGATGA CGATAATGGA 
AACATCGATC TGAGCGAGTC CGATGACTTT TTGCGGGAGG AATTGAAGTA CGACTCGGGC 
TACGAAAAGC GGCAGAAAGC GTTTCACTTC AATGACGATA TGCATATATC GGTCAAAGAA 
CTTTGGGAGG CCTGGCTCAG ATCGGAGGTG CATAATTGGA CCATCGAGCA GACCACCGAT 
TGGCTGGCTC AGTCCGTTCA GCTGCCGCAA TACGTTGATC TGTTCAAATT ACACAAGGTT 
ACTGGCGCTG CCTTGCCAAG ATTGGCTGTG AATAATCTTC AGTATGTTGG CAATGTACTT 
GGCATCAAAG ACCCTATACA CAAACAAAAA ATCTCATTGA AGGCAATGGA TGTGGTTCTG 
TTTGGGCCAC CGCGAGAAAC TGGTACCCGC TGGAAAGACT ACATATTGGT AACACTGTTG 
CTTAGTGCTA TTATTGGTTG TTGGTACGCC TATCAGCAAA ATAAGAATGC CAAACGGCAT 
CTGCGTCGAA TGGCCCAGGA TATGGAGGGA TTGCAGAGGG CTGAGCAAAG TCTACAGGAG 
ATGCAGAAGG AACTAGAACG GGCCAGAATG GAGCAGGAAA ATGTGGCAAC AGAAAAACTA 
GATTTGGAGC GTCGTCTAAA AGAAGCGCCC ACTCTCAGTT CATCGAACTC GGATTTGGAA 
GTTCAGCAGC TGAAAAAGGA AATCGAGATG TTGCGCAACG AATTGTCCCG CGCCGAATTC 
GAGCTAGTAG ACAACTGCTG GTCACCGCCG CCACAACTGC AATCATGGCT TCAATACACA 
TATGAACTAG AAAGTAAGAA TCATCAGAAG AAGCGCACGT CGGCTGAGAA GCAGCTACAG 
TCGGCCAGAG AGGCTTGTGA GAAATTGCGT AAGAAACGGT CAAGTTTGGT GGGTGCGTTC 
GTTTCCACGC ACGGAAAGAG TATTGATGAT GTGGATCGGT CGATTGTTGA GGCACGGAAT 
GCCCTCGGAG ATGTAACAAA CGAGCTGCAA GAACGACTGC ATCGCTGGAA GCAAATCGAG 
ACGTGCCTTG GCTTAAACAT TGTGAACAAC AATGGTCTGC CCTACTTGGA GAATGTTCTG 
TACGGTCGAA ATGGGGGCTT ACAAAGTTCC ATGGGCATGA GTTCAACCAA GGGTTCTAGA 
GCACGTATTA CCAACAGCAC CGAAGACCTG GACGATGAGT CCATACAAGG TTTTGGAATT 
CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stim ( NM_078633.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523357.2 Drosophila melanogaster stromal interaction molecule, transcript variant A (Stim), mRNA.

Price & Availability↑

CloneID	OFa01735
Clone ID Related Accession (Same CDS sequence)	NM_078633.4
Accession Version	NM_078633.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stromal interaction molecule, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078633.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAAAGA ATACCATTTG GAACTACTCT TTAATATTCT TCTGCTGTGT GCTGAAGAGC 
ATAAGTACGC TAGATCATGG CCCGCACACA GTATCAGTCG ATTCGAATCG ACACAACACA 
CAGCATCAGT ATAAGCAAAA TCCCAATGTT GCCTCACAAC GTCACTCATC CCACGAATCT 
GGTCAGAGTT TACACAATTC GCAATCGGAA CATGTCACCC ATATTGCCGC ATCGCACGCC 
GGAAGCGGCG GAGAGCACTC CACTCATTTG GCGCAAAATC TGCACAGGAG CTCATATAAT 
CTTCTGAGCG AGGCCATGTC CCAGGCTGTC AGCAATGAAT TTAGTTCCAT GGGAAGTGGT 
TCAGCGGATG GAGCGTGTGC TGCTGATGAT TTTGATTGCT ACAGTGGAAG TGTTCAGGAT 
CGCTTTGGCA TGGAGGCTAT TGCCAGCTTG CATCGTCAGC TAGATGATGA CGATAATGGA 
AACATCGATC TGAGCGAGTC CGATGACTTT TTGCGGGAGG AATTGAAGTA CGACTCGGGC 
TACGAAAAGC GGCAGAAAGC GTTTCACTTC AATGACGATA TGCATATATC GGTCAAAGAA 
CTTTGGGAGG CCTGGCTCAG ATCGGAGGTG CATAATTGGA CCATCGAGCA GACCACCGAT 
TGGCTGGCTC AGTCCGTTCA GCTGCCGCAA TACGTTGATC TGTTCAAATT ACACAAGGTT 
ACTGGCGCTG CCTTGCCAAG ATTGGCTGTG AATAATCTTC AGTATGTTGG CAATGTACTT 
GGCATCAAAG ACCCTATACA CAAACAAAAA ATCTCATTGA AGGCAATGGA TGTGGTTCTG 
TTTGGGCCAC CGCGAGAAAC TGGTACCCGC TGGAAAGACT ACATATTGGT AACACTGTTG 
CTTAGTGCTA TTATTGGTTG TTGGTACGCC TATCAGCAAA ATAAGAATGC CAAACGGCAT 
CTGCGTCGAA TGGCCCAGGA TATGGAGGGA TTGCAGAGGG CTGAGCAAAG TCTACAGGAG 
ATGCAGAAGG AACTAGAACG GGCCAGAATG GAGCAGGAAA ATGTGGCAAC AGAAAAACTA 
GATTTGGAGC GTCGTCTAAA AGAAGCGCCC ACTCTCAGTT CATCGAACTC GGATTTGGAA 
GTTCAGCAGC TGAAAAAGGA AATCGAGATG TTGCGCAACG AATTGTCCCG CGCCGAATTC 
GAGCTAGTAG ACAACTGCTG GTCACCGCCG CCACAACTGC AATCATGGCT TCAATACACA 
TATGAACTAG AAAGTAAGAA TCATCAGAAG AAGCGCACGT CGGCTGAGAA GCAGCTACAG 
TCGGCCAGAG AGGCTTGTGA GAAATTGCGT AAGAAACGGT CAAGTTTGGT GGGTGCGTTC 
GTTTCCACGC ACGGAAAGAG TATTGATGAT GTGGATCGGT CGATTGTTGA GGCACGGAAT 
GCCCTCGGAG ATGTAACAAA CGAGCTGCAA GAACGACTGC ATCGCTGGAA GCAAATCGAG 
ACGTGCCTTG GCTTAAACAT TGTGAACAAC AATGGTCTGC CCTACTTGGA GAATGTTCTG 
TACGGTCGAA ATGGGGGCTT ACAAAGTTCC ATGGGCATGA GTTCAACCAA GGGTTCTAGA 
GCACGTATTA CCAACAGCAC CGAAGACCTG GACGATGAGT CCATACAAGG TAAGCTGAAT 
TTTGAGAACT TTTCGCTGCT TGCCACGGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523357.2
CDS	193..1905
Translation	MRKNTIWNYSLIFFCCVLKSISTLDHGPHTVSVDSNRHNTQHQYKQNPNVASQRHSSHESGQSLHNSQSEHVTHIAASHAGSGGEHSTHLAQNLHRSSYNLLSEAMSQAVSNEFSSMGSGSADGACAADDFDCYSGSVQDRFGMEAIASLHRQLDDDDNGNIDLSESDDFLREELKYDSGYEKRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKLHKVTGAALPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKDYILVTLLLSAIIGCWYAYQQNKNAKRHLRRMAQDMEGLQRAEQSLQEMQKELERARMEQENVATEKLDLERRLKEAPTLSSSNSDLEVQQLKKEIEMLRNELSRAEFELVDNCWSPPPQLQSWLQYTYELESKNHQKKRTSAEKQLQSAREACEKLRKKRSSLVGAFVSTHGKSIDDVDRSIVEARNALGDVTNELQERLHRWKQIETCLGLNIVNNNGLPYLENVLYGRNGGLQSSMGMSSTKGSRARITNSTEDLDDESIQGKLNFENFSLLATE

Target ORF information:

RefSeq Version	NM_078633.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stromal interaction molecule, transcript variant A (Stim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078633.4

ORF Insert Sequence:

ATGCGAAAGA ATACCATTTG GAACTACTCT TTAATATTCT TCTGCTGTGT GCTGAAGAGC 
ATAAGTACGC TAGATCATGG CCCGCACACA GTATCAGTCG ATTCGAATCG ACACAACACA 
CAGCATCAGT ATAAGCAAAA TCCCAATGTT GCCTCACAAC GTCACTCATC CCACGAATCT 
GGTCAGAGTT TACACAATTC GCAATCGGAA CATGTCACCC ATATTGCCGC ATCGCACGCC 
GGAAGCGGCG GAGAGCACTC CACTCATTTG GCGCAAAATC TGCACAGGAG CTCATATAAT 
CTTCTGAGCG AGGCCATGTC CCAGGCTGTC AGCAATGAAT TTAGTTCCAT GGGAAGTGGT 
TCAGCGGATG GAGCGTGTGC TGCTGATGAT TTTGATTGCT ACAGTGGAAG TGTTCAGGAT 
CGCTTTGGCA TGGAGGCTAT TGCCAGCTTG CATCGTCAGC TAGATGATGA CGATAATGGA 
AACATCGATC TGAGCGAGTC CGATGACTTT TTGCGGGAGG AATTGAAGTA CGACTCGGGC 
TACGAAAAGC GGCAGAAAGC GTTTCACTTC AATGACGATA TGCATATATC GGTCAAAGAA 
CTTTGGGAGG CCTGGCTCAG ATCGGAGGTG CATAATTGGA CCATCGAGCA GACCACCGAT 
TGGCTGGCTC AGTCCGTTCA GCTGCCGCAA TACGTTGATC TGTTCAAATT ACACAAGGTT 
ACTGGCGCTG CCTTGCCAAG ATTGGCTGTG AATAATCTTC AGTATGTTGG CAATGTACTT 
GGCATCAAAG ACCCTATACA CAAACAAAAA ATCTCATTGA AGGCAATGGA TGTGGTTCTG 
TTTGGGCCAC CGCGAGAAAC TGGTACCCGC TGGAAAGACT ACATATTGGT AACACTGTTG 
CTTAGTGCTA TTATTGGTTG TTGGTACGCC TATCAGCAAA ATAAGAATGC CAAACGGCAT 
CTGCGTCGAA TGGCCCAGGA TATGGAGGGA TTGCAGAGGG CTGAGCAAAG TCTACAGGAG 
ATGCAGAAGG AACTAGAACG GGCCAGAATG GAGCAGGAAA ATGTGGCAAC AGAAAAACTA 
GATTTGGAGC GTCGTCTAAA AGAAGCGCCC ACTCTCAGTT CATCGAACTC GGATTTGGAA 
GTTCAGCAGC TGAAAAAGGA AATCGAGATG TTGCGCAACG AATTGTCCCG CGCCGAATTC 
GAGCTAGTAG ACAACTGCTG GTCACCGCCG CCACAACTGC AATCATGGCT TCAATACACA 
TATGAACTAG AAAGTAAGAA TCATCAGAAG AAGCGCACGT CGGCTGAGAA GCAGCTACAG 
TCGGCCAGAG AGGCTTGTGA GAAATTGCGT AAGAAACGGT CAAGTTTGGT GGGTGCGTTC 
GTTTCCACGC ACGGAAAGAG TATTGATGAT GTGGATCGGT CGATTGTTGA GGCACGGAAT 
GCCCTCGGAG ATGTAACAAA CGAGCTGCAA GAACGACTGC ATCGCTGGAA GCAAATCGAG 
ACGTGCCTTG GCTTAAACAT TGTGAACAAC AATGGTCTGC CCTACTTGGA GAATGTTCTG 
TACGGTCGAA ATGGGGGCTT ACAAAGTTCC ATGGGCATGA GTTCAACCAA GGGTTCTAGA 
GCACGTATTA CCAACAGCAC CGAAGACCTG GACGATGAGT CCATACAAGG TAAGCTGAAT 
TTTGAGAACT TTTCGCTGCT TGCCACGGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stim ( NM_001258774.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245703.1 Drosophila melanogaster stromal interaction molecule, transcript variant F (Stim), mRNA.

Price & Availability↑

CloneID	OFa03623
Clone ID Related Accession (Same CDS sequence)	NM_001258774.2 , NM_001298380.1
Accession Version	NM_001258774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stromal interaction molecule, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258774.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAAAGA ATACCATTTG GAACTACTCT TTAATATTCT TCTGCTGTGT GCTGAAGAGC 
ATAAGTACGC TAGATCATGG CCCGCACACA GTATCAGTCG ATTCGAATCG ACACAACACA 
CAGCATCAGT ATAAGCAAAA TCCCAATGTT GCCTCACAAC GTCACTCATC CCACGAATCT 
GGTCAGAGTT TACACAATTC GCAATCGGAA CATGTCACCC ATATTGCCGC ATCGCACGCC 
GGAAGCGGCG GAGAGCACTC CACTCATTTG GCGCAAAATC TGCACAGGAG CTCATATAAT 
CTTCTGAGCG AGGCCATGTC CCAGGCTGTC AGCAATGAAT TTAGTAGTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245703.1
CDS	153..506
Translation	MRKNTIWNYSLIFFCCVLKSISTLDHGPHTVSVDSNRHNTQHQYKQNPNVASQRHSSHESGQSLHNSQSEHVTHIAASHAGSGGEHSTHLAQNLHRSSYNLLSEAMSQAVSNEFSSI

Target ORF information:

RefSeq Version	NM_001258774.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stromal interaction molecule, transcript variant F (Stim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258774.2

ORF Insert Sequence:

ATGCGAAAGA ATACCATTTG GAACTACTCT TTAATATTCT TCTGCTGTGT GCTGAAGAGC 
ATAAGTACGC TAGATCATGG CCCGCACACA GTATCAGTCG ATTCGAATCG ACACAACACA 
CAGCATCAGT ATAAGCAAAA TCCCAATGTT GCCTCACAAC GTCACTCATC CCACGAATCT 
GGTCAGAGTT TACACAATTC GCAATCGGAA CATGTCACCC ATATTGCCGC ATCGCACGCC 
GGAAGCGGCG GAGAGCACTC CACTCATTTG GCGCAAAATC TGCACAGGAG CTCATATAAT 
CTTCTGAGCG AGGCCATGTC CCAGGCTGTC AGCAATGAAT TTAGTAGTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stim ( NM_001298380.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285309.1 Drosophila melanogaster stromal interaction molecule, transcript variant G (Stim), mRNA.

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CloneID	OFa03623
Clone ID Related Accession (Same CDS sequence)	NM_001258774.2 , NM_001298380.1
Accession Version	NM_001298380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stromal interaction molecule, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAAAGA ATACCATTTG GAACTACTCT TTAATATTCT TCTGCTGTGT GCTGAAGAGC 
ATAAGTACGC TAGATCATGG CCCGCACACA GTATCAGTCG ATTCGAATCG ACACAACACA 
CAGCATCAGT ATAAGCAAAA TCCCAATGTT GCCTCACAAC GTCACTCATC CCACGAATCT 
GGTCAGAGTT TACACAATTC GCAATCGGAA CATGTCACCC ATATTGCCGC ATCGCACGCC 
GGAAGCGGCG GAGAGCACTC CACTCATTTG GCGCAAAATC TGCACAGGAG CTCATATAAT 
CTTCTGAGCG AGGCCATGTC CCAGGCTGTC AGCAATGAAT TTAGTAGTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285309.1
CDS	193..546
Translation	MRKNTIWNYSLIFFCCVLKSISTLDHGPHTVSVDSNRHNTQHQYKQNPNVASQRHSSHESGQSLHNSQSEHVTHIAASHAGSGGEHSTHLAQNLHRSSYNLLSEAMSQAVSNEFSSI

Target ORF information:

RefSeq Version	NM_001298380.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stromal interaction molecule, transcript variant G (Stim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298380.1

ORF Insert Sequence:

ATGCGAAAGA ATACCATTTG GAACTACTCT TTAATATTCT TCTGCTGTGT GCTGAAGAGC 
ATAAGTACGC TAGATCATGG CCCGCACACA GTATCAGTCG ATTCGAATCG ACACAACACA 
CAGCATCAGT ATAAGCAAAA TCCCAATGTT GCCTCACAAC GTCACTCATC CCACGAATCT 
GGTCAGAGTT TACACAATTC GCAATCGGAA CATGTCACCC ATATTGCCGC ATCGCACGCC 
GGAAGCGGCG GAGAGCACTC CACTCATTTG GCGCAAAATC TGCACAGGAG CTCATATAAT 
CTTCTGAGCG AGGCCATGTC CCAGGCTGTC AGCAATGAAT TTAGTAGTAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Stim cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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